FvH4_1g14870

salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000171
Physical Location & Seq
Forward (+)
8294055 .. 8295542
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14870.t4

Sequence Viewer

Length: 849 bp
ATGCATGGAACAGAAAGAGATCGATCAGTTTATATGAAAATGGAGAATTCAAAGCATTTTGTTCTGGTTCATGGTTCTGGTCATGGAGCGTGGTGTTGGTATAAGCTGGCAACTCTATTGAGCTCCACAGGTCACAATGTTACAACTCTAGACCTTCCGGCATCCGGGATCAACCAAACACAGCAACAACAACTTCATTCGTTTTCCGACTACGCTGAGCCATTGTTCGAATTTCTGGGGTCTCTGCCACCAGAAGAGAAGGTTATCCTTGTCGGACACAGCATGGGCGGCCCCGTCATATCGATCGCCATGGAGATGTTCCCTGAGAAAATTGCTGCTGCTGTATTTGCTACTGCTTTCATGCCTGGTCCTGACTTCAGTTACTTGACCATTTATCAAAAGTTGGTAGAGAGAACGGTGGTTTCAGACTCCCAGTTTATAAATGATCAAGGTGTCGACAATCCTCCATCCGCAATGATCTTTGGGCCAAAGCGGTTGGCGACGAAGCTGTACCAGCTCTCACCACCAGAGGATTTAACACTGGCATTGTCTTTGGTGAGAATTTTCCCATTGTTTAAAGAAGACATAAAACTCAGCAAGGAGAAATATGGATCGGTTCCAAGAGTGTTCATCGTTTCTGACCAAGACCTTTCAATAGAGGAGGATGCGCAAATGTGGATGATAGAGAATAATCCACCAAATGAAGTGAAAGTTATAAATGGTGCGGATCACATGGTCATGTTGCTGGGAAATATGCTTAATTACCTGGTACTTGTTTACCTGTACACATGCATTGTTGATGAAAAGTATGATCGAATCACTTTTCATAAAATAAAGTCACCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

283

Amino Acids

31.81

Weight (kDa)

5.46

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 18 - 131 4.1e-12 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 19 - 128 1.4e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 20 - 129 2.1e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 440, 716
Acc16I TGCGCA 1 cut(s) 669
AccI GTMKAC 1 cut(s) 456
AciI CCGC 4 cut(s) 288, 471, 493, 725
AclWI GGATC 3 cut(s) 176, 619, 735
AcsI RAATTY 3 cut(s) 46, 230, 561
AcuI CTGAAG 1 cut(s) 361
AfaI GTAC 3 cut(s) 512, 771, 785
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 2 cut(s) 51, 654
AjnI CCWGG 2 cut(s) 364, 765
AluBI AGCT 4 cut(s) 106, 123, 508, 517
AluI AGCT 4 cut(s) 106, 123, 508, 517
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 3 cut(s) 176, 619, 735
AoxI GGCC 2 cut(s) 289, 485
ApeKI GCWGC 2 cut(s) 335, 338
ApoI RAATTY 3 cut(s) 46, 230, 561
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 3 cut(s) 290, 368, 485
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 513, 568, 831
AsuII TTCGAA 1 cut(s) 228
AvaII GGWCC 1 cut(s) 368
BanII GRGCYC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 2 cut(s) 322, 325
BccI CCATC 1 cut(s) 475
BciT130I CCWGG 2 cut(s) 366, 767
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 149
BisI GCNGC 3 cut(s) 289, 336, 339
BlpI GCTNAGC 1 cut(s) 216
BlsI GCNGC 3 cut(s) 290, 337, 340
Bme1390I CCNGG 3 cut(s) 166, 366, 767
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 290, 368, 485
BmiI GGNNCC 2 cut(s) 292, 618
BmrFI CCNGG 3 cut(s) 166, 366, 767
BmrI ACTGGG 1 cut(s) 427
BmsI GCATC 2 cut(s) 170, 655
BmuI ACTGGG 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 588
Bpu1102I GCTNAGC 1 cut(s) 216
Bpu14I TTCGAA 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 166
Bsa29I ATCGAT 2 cut(s) 22, 302
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 433, 546
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 2 cut(s) 366, 767
BseCI ATCGAT 2 cut(s) 22, 302
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 4 cut(s) 161, 467, 670, 684
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 3 cut(s) 207, 315, 607
BseNI ACTGG 2 cut(s) 433, 546
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 2 cut(s) 322, 325
BseYI CCCAGC 1 cut(s) 745
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 2 cut(s) 291, 487
BshVI ATCGAT 2 cut(s) 22, 302
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 2 cut(s) 158, 165
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 246
BsnI GGCC 2 cut(s) 291, 487
Bso31I GGTCTC 1 cut(s) 246
Bsp119I TTCGAA 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 125
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
Bsp1720I GCTNAGC 1 cut(s) 216
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 4 cut(s) 288, 471, 493, 725
BspANI GGCC 2 cut(s) 291, 487
BspCNI CTCAG 3 cut(s) 208, 316, 606
BspDI ATCGAT 2 cut(s) 22, 302
BspLI GGNNCC 2 cut(s) 292, 618
BspPI GGATC 3 cut(s) 176, 619, 735
BspT104I TTCGAA 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 480
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 2 cut(s) 433, 546
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 366, 767
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 1 cut(s) 249
BstAUI TGTACA 1 cut(s) 783
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 3 cut(s) 216, 324, 593
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 4 cut(s) 161, 467, 670, 684
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 9 cut(s) 22, 26, 171, 306, 448, 480, 614, 730, 814
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 3 cut(s) 288, 347, 514
BstNI CCWGG 2 cut(s) 366, 767
BstNSI RCATGY 1 cut(s) 792
BstSCI CCNGG 3 cut(s) 164, 364, 765
BstV1I GCAGC 2 cut(s) 322, 325
BstV2I GAAGAC 1 cut(s) 588
Bsu15I ATCGAT 2 cut(s) 22, 302
BsuRI GGCC 2 cut(s) 291, 487
BsuTUI ATCGAT 2 cut(s) 22, 302
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 4 cut(s) 161, 467, 670, 684
BtsIMutI CAGTG 1 cut(s) 539
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 3 cut(s) 290, 368, 485
ClaI ATCGAT 2 cut(s) 22, 302
CsiI ACCWGGT 1 cut(s) 765
Csp6I GTAC 3 cut(s) 511, 770, 784
CviAII CATG 9 cut(s) 5, 71, 83, 283, 310, 361, 733, 739, 789
CviJI RGCY 7 cut(s) 106, 123, 220, 291, 487, 508, 517
CviKI_1 RGCY 7 cut(s) 106, 123, 220, 291, 487, 508, 517
CviQI GTAC 3 cut(s) 511, 770, 784
DdeI CTNAG 3 cut(s) 216, 324, 593
DpnI GATC 9 cut(s) 21, 25, 170, 305, 447, 479, 613, 729, 813
DpnII GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 364, 765
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 2 cut(s) 6, 794
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 9 cut(s) 8, 74, 86, 286, 313, 364, 736, 742, 792
FatI CATG 9 cut(s) 4, 70, 82, 282, 309, 360, 732, 738, 788
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 3 cut(s) 289, 336, 339
FokI GGATG 4 cut(s) 148, 454, 677, 691
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 3 cut(s) 289, 336, 339
FspBI CTAG 1 cut(s) 149
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 1 cut(s) 669
GluI GCNGC 3 cut(s) 289, 336, 339
GsaI CCCAGC 1 cut(s) 749
HaeIII GGCC 2 cut(s) 291, 487
HapII CCGG 2 cut(s) 158, 165
HhaI GCGC 1 cut(s) 670
Hin1II CATG 9 cut(s) 8, 74, 86, 286, 313, 364, 736, 742, 792
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 2 cut(s) 428, 816
HpaII CCGG 2 cut(s) 158, 165
HphI GGTGA 3 cut(s) 513, 568, 831
Hpy166II GTNNAC 3 cut(s) 457, 778, 786
Hpy188I TCNGA 4 cut(s) 208, 275, 427, 640
Hpy188III TCNNGA 2 cut(s) 149, 371
Hpy8I GTNNAC 3 cut(s) 457, 778, 786
Hpy99I CGWCG 1 cut(s) 505
HpyAV CCTTC 2 cut(s) 164, 253
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4V TGCA 2 cut(s) 4, 792
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 347, 514
HpyF3I CTNAG 3 cut(s) 216, 324, 593
Hsp92II CATG 9 cut(s) 8, 74, 86, 286, 313, 364, 736, 742, 792
HspAI GCGC 1 cut(s) 668
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
LmnI GCTCC 2 cut(s) 86, 128
Lsp1109I GCAGC 2 cut(s) 322, 325
LweI GCATC 2 cut(s) 170, 655
MabI ACCWGGT 1 cut(s) 765
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 4 cut(s) 131, 139, 380, 837
MalI GATC 9 cut(s) 21, 25, 170, 305, 447, 479, 613, 729, 813
MboI GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
MboII GAAGA 2 cut(s) 266, 593
MhlI GDGCHC 1 cut(s) 125
MluCI AATT 5 cut(s) 46, 230, 330, 561, 760
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 2 cut(s) 231, 253
MnlI CCTC 4 cut(s) 474, 523, 652, 655
Mph1103I ATGCAT 2 cut(s) 6, 794
MseI TTAA 3 cut(s) 536, 576, 759
MslI CAYNNNNRTG 2 cut(s) 314, 737
MspI CCGG 2 cut(s) 158, 165
MspR9I CCNGG 3 cut(s) 166, 366, 767
MvaI CCWGG 2 cut(s) 366, 767
MwoI GCNNNNNNNGC 3 cut(s) 288, 347, 514
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 309
NdeII GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
NlaIII CATG 9 cut(s) 8, 74, 86, 286, 313, 364, 736, 742, 792
NlaIV GGNNCC 2 cut(s) 292, 618
NmuCI GTSAC 2 cut(s) 131, 837
NsbI TGCGCA 1 cut(s) 669
NsiI ATGCAT 2 cut(s) 6, 794
NspI RCATGY 1 cut(s) 792
NspV TTCGAA 1 cut(s) 228
PfeI GAWTC 1 cut(s) 816
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 3 cut(s) 290, 337, 340
Ple19I CGATCG 1 cut(s) 306
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
PsiI TTATAA 2 cut(s) 440, 716
Psp124BI GAGCTC 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 364, 765
PspFI CCCAGC 1 cut(s) 745
PspGI CCWGG 2 cut(s) 364, 765
PspN4I GGNNCC 2 cut(s) 292, 618
PspPI GGNCC 3 cut(s) 290, 368, 485
PvuI CGATCG 1 cut(s) 306
RsaI GTAC 3 cut(s) 512, 771, 785
RsaNI GTAC 3 cut(s) 511, 770, 784
RseI CAYNNNNRTG 2 cut(s) 314, 737
SacI GAGCTC 1 cut(s) 125
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 3 cut(s) 536, 576, 759
SatI GCNGC 3 cut(s) 289, 336, 339
Sau3AI GATC 9 cut(s) 19, 23, 168, 303, 445, 477, 611, 727, 811
Sau96I GGNCC 3 cut(s) 290, 368, 485
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 3 cut(s) 166, 366, 767
SduI GDGCHC 1 cut(s) 125
SexAI ACCWGGT 1 cut(s) 765
SfaNI GCATC 2 cut(s) 170, 655
SfuI TTCGAA 1 cut(s) 228
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 2 cut(s) 314, 737
Sse9I AATT 5 cut(s) 46, 230, 330, 561, 760
SsiI CCGC 4 cut(s) 288, 471, 493, 725
SspMI CTAG 1 cut(s) 149
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 164, 364, 765
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 418
TaqI TCGA 5 cut(s) 22, 228, 302, 456, 814
TasI AATT 5 cut(s) 46, 230, 330, 561, 760
TatI WGTACW 1 cut(s) 783
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 1 cut(s) 816
Tru1I TTAA 3 cut(s) 536, 576, 759
Tru9I TTAA 3 cut(s) 536, 576, 759
TscAI CASTG 1 cut(s) 546
TseFI GTSAC 2 cut(s) 131, 837
TseI GCWGC 2 cut(s) 335, 338
Tsp45I GTSAC 2 cut(s) 131, 837
TspDTI ATGAA 8 cut(s) 50, 59, 185, 349, 619, 717, 815, 816
TspRI CASTG 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 3 cut(s) 46, 230, 561
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 1 cut(s) 792
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 1 cut(s) 149
Zsp2I ATGCAT 2 cut(s) 6, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.