Rh2CG173200

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000171
Physical Location & Seq
Forward (+)
15472355 .. 15509141
36787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG173200.1

Sequence Viewer

Length: 939 bp
ATGGCAGCATCCGGAATAAACCCGATGCAGGTGCAAGAAGTCCATTCGTTCTCTGATTATGCTGAGCCGTTGATCAAATTCATGGCTTCTCTACCACCACTACATAGGGTTATCCTAGTTGGCCACAGCATGGGTGGTGCCATCATATCTATTGCCATGGAGATGTTCCCTCAGAGGATTTCAGCTGCTGTATTCGCCACCGCTGCCATGCCTGGGCCTGCTCTACATTACTCCACTATAGTTGCAACGATCAACAGTGGTGCGAGTACTCTTACGGACTCATATACAGTAAATGATGGGCCAAACAACCCTCCGACCTCTTTACTATTTGGGTCTAACTTCATGTCCTCATCGCTGTATCAGCTCTCACCACCAGAGGACTTAACATTAGCGTCCTCATTGGTGCGACCTTCCCCTCTCTTTAATGAAGAAATAAAACTCACAAAGCTGAAATATGGAATGGTTCGTAGACTACTCATCGCATGTGACCAAGACCGTGGATATACCGAGCAAGTGGTAAGGTTGATGATCAGTCAAAATCGGCCGAATGAAGTGAAAGTGATCAATGGTTCAGATCACATAGTTATGGTCTCTAGACCAGTAGAGCTGTTCTCCCATCTCCAGAAGATTTCGGTGAAAAAGCTCAATCACGAGTCACAGACGACGAACAGAATACAAGATTGGATGATTGCGCGTCGGCTTTTTGGAATGTGGGCGGCTGAGGTATCTTTTCTCGGTTTTGGGATTTGGCCAACGCAAAACTTCCACACGAAAACTGAAAGTCTGAAACAAACGAAGCCTACACAAACCCGAAAGCCATTTCCGACGACCCTAATAAGGAGAAGTACTTTTAGCCCAAGCAAACGTCTCATCCTTCGCCTTCAAGCTACGGAAATGATTGACAAGGACCTGACAGTTGAAAGCCTCATACAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

312

Amino Acids

34.83

Weight (kDa)

9.54

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 13 - 97 7.5e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 17 - 203 2.4e-11 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 18 - 194 2.5e-08 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 1 cut(s) 469
AccII CGCG 1 cut(s) 694
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 2 cut(s) 201, 716
AcoI YGGCCR 3 cut(s) 121, 542, 749
AcsI RAATTY 1 cut(s) 77
AfaI GTAC 2 cut(s) 268, 847
AfiI CCNNNNNNNGG 4 cut(s) 28, 130, 213, 837
AgsI TTSAA 2 cut(s) 884, 920
AjnI CCWGG 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 664, 696
AluBI AGCT 6 cut(s) 185, 364, 448, 607, 643, 887
AluI AGCT 6 cut(s) 185, 364, 448, 607, 643, 887
Alw26I GTCTC 2 cut(s) 595, 872
AlwNI CAGNNNCTG 1 cut(s) 188
Aor13HI TCCGGA 1 cut(s) 11
AoxI GGCC 5 cut(s) 121, 215, 299, 542, 749
ApeKI GCWGC 3 cut(s) 5, 185, 203
ApoI RAATTY 1 cut(s) 77
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 3 cut(s) 215, 299, 907
AsuHPI GGTGA 2 cut(s) 360, 646
AvaII GGWCC 1 cut(s) 907
BalI TGGCCA 2 cut(s) 123, 751
BanI GGYRCC 1 cut(s) 137
BauI CACGAG 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 720
BbvI GCAGC 3 cut(s) 17, 172, 190
BccI CCATC 3 cut(s) 149, 290, 624
BceAI ACGGC 1 cut(s) 52
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 1 cut(s) 213
BclI TGATCA 3 cut(s) 72, 528, 561
BcoDI GTCTC 2 cut(s) 595, 872
BfaI CTAG 2 cut(s) 116, 594
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 4 cut(s) 6, 186, 204, 717
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 4 cut(s) 7, 187, 205, 718
BmcAI AGTACT 2 cut(s) 268, 847
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 907
BmgT120I GGNCC 3 cut(s) 215, 299, 907
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 2 cut(s) 15, 17
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 720
Bpu1102I GCTNAGC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 595
BsaJI CCNNGG 3 cut(s) 156, 212, 496
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 130, 213, 837
Bse1I ACTGG 1 cut(s) 599
BseAI TCCGGA 1 cut(s) 11
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 156, 212, 496
BseGI GGATG 3 cut(s) 8, 690, 870
BseLI CCNNNNNNNGG 4 cut(s) 28, 130, 213, 837
BseMII CTCAG 3 cut(s) 54, 185, 711
BseNI ACTGG 1 cut(s) 599
BseX3I CGGCCG 1 cut(s) 542
BseXI GCAGC 3 cut(s) 17, 172, 190
Bsh1236I CGCG 1 cut(s) 694
Bsh1285I CGRYCG 1 cut(s) 545
BshFI GGCC 5 cut(s) 123, 217, 301, 544, 751
BshNI GGYRCC 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 545
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 4 cut(s) 28, 130, 213, 837
BsmAI GTCTC 2 cut(s) 595, 872
BsmBI CGTCTC 1 cut(s) 872
BsnI GGCC 5 cut(s) 123, 217, 301, 544, 751
Bso31I GGTCTC 1 cut(s) 595
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 5 cut(s) 72, 249, 528, 561, 574
Bsp1720I GCTNAGC 1 cut(s) 63
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 2 cut(s) 201, 716
BspANI GGCC 5 cut(s) 123, 217, 301, 544, 751
BspCNI CTCAG 3 cut(s) 55, 184, 712
BspEI TCCGGA 1 cut(s) 11
BspFNI CGCG 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 139
BspMI ACCTGC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 595
BsrI ACTGG 1 cut(s) 599
BssECI CCNNGG 3 cut(s) 156, 212, 496
BssMI GATC 5 cut(s) 72, 249, 528, 561, 574
BssSI CACGAG 1 cut(s) 650
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 4 cut(s) 257, 289, 497, 916
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 3 cut(s) 63, 171, 720
BstDSI CCRYGG 2 cut(s) 156, 496
BstF5I GGATG 3 cut(s) 8, 690, 870
BstFNI CGCG 1 cut(s) 694
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 5 cut(s) 75, 252, 531, 564, 577
BstMAI GTCTC 2 cut(s) 595, 872
BstMBI GATC 5 cut(s) 72, 249, 528, 561, 574
BstMCI CGRYCG 1 cut(s) 545
BstMWI GCNNNNNNNGC 3 cut(s) 194, 203, 361
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 237
BstUI CGCG 1 cut(s) 694
BstV1I GCAGC 3 cut(s) 17, 172, 190
BstXI CCANNNNNNTGG 1 cut(s) 497
BstZI CGGCCG 1 cut(s) 542
BsuRI GGCC 5 cut(s) 123, 217, 301, 544, 751
BtgI CCRYGG 2 cut(s) 156, 496
BtgZI GCGATG 2 cut(s) 336, 463
BtsCI GGATG 3 cut(s) 8, 690, 870
BtsIMutI CAGTG 1 cut(s) 262
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 219
CaiI CAGNNNCTG 1 cut(s) 188
CfoI GCGC 1 cut(s) 694
Cfr13I GGNCC 3 cut(s) 215, 299, 907
CseI GACGC 2 cut(s) 381, 683
Csp6I GTAC 2 cut(s) 267, 846
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 6 cut(s) 82, 130, 157, 208, 343, 483
CviQI GTAC 2 cut(s) 267, 846
DdeI CTNAG 3 cut(s) 63, 171, 720
DpnI GATC 5 cut(s) 74, 251, 530, 563, 576
DpnII GATC 5 cut(s) 72, 249, 528, 561, 574
EaeI YGGCCR 3 cut(s) 121, 542, 749
EagI CGGCCG 1 cut(s) 542
EclXI CGGCCG 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 907
Eco52I CGGCCG 1 cut(s) 542
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 872
FaeI CATG 6 cut(s) 85, 133, 160, 211, 346, 486
FatI CATG 6 cut(s) 81, 129, 156, 207, 342, 482
FbaI TGATCA 3 cut(s) 72, 528, 561
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 4 cut(s) 6, 186, 204, 717
FokI GGATG 2 cut(s) 697, 857
Fsp4HI GCNGC 4 cut(s) 6, 186, 204, 717
FspBI CTAG 2 cut(s) 116, 594
GlaI GCGC 1 cut(s) 693
GluI GCNGC 4 cut(s) 6, 186, 204, 717
GsuI CTGGAG 1 cut(s) 605
HaeIII GGCC 5 cut(s) 123, 217, 301, 544, 751
HapII CCGG 1 cut(s) 12
HgaI GACGC 2 cut(s) 381, 683
HhaI GCGC 1 cut(s) 694
Hin1II CATG 6 cut(s) 85, 133, 160, 211, 346, 486
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HinfI GANTC 2 cut(s) 278, 653
HpaII CCGG 1 cut(s) 12
HphI GGTGA 2 cut(s) 360, 646
Hpy166II GTNNAC 1 cut(s) 470
Hpy188I TCNGA 6 cut(s) 55, 174, 315, 574, 786, 825
Hpy188III TCNNGA 4 cut(s) 12, 594, 622, 650
Hpy8I GTNNAC 1 cut(s) 470
Hpy99I CGWCG 3 cut(s) 667, 699, 829
HpyAV CCTTC 3 cut(s) 420, 884, 890
HpyCH4III ACNGT 4 cut(s) 257, 289, 497, 916
HpyCH4IV ACGT 1 cut(s) 865
HpyCH4V TGCA 3 cut(s) 28, 34, 245
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 203, 361
HpyF3I CTNAG 3 cut(s) 63, 171, 720
HpySE526I ACGT 1 cut(s) 865
Hsp92II CATG 6 cut(s) 85, 133, 160, 211, 346, 486
HspAI GCGC 1 cut(s) 692
Kpn2I TCCGGA 1 cut(s) 11
Ksp22I TGATCA 3 cut(s) 72, 528, 561
Kzo9I GATC 5 cut(s) 72, 249, 528, 561, 574
LpnPI CCDG 9 cut(s) 14, 25, 198, 225, 231, 387, 612, 635, 923
Lsp1109I GCAGC 3 cut(s) 17, 172, 190
LweI GCATC 2 cut(s) 15, 17
MaeI CTAG 2 cut(s) 116, 594
MaeII ACGT 1 cut(s) 865
MaeIII GTNAC 2 cut(s) 485, 654
MalI GATC 5 cut(s) 74, 251, 530, 563, 576
MboI GATC 5 cut(s) 72, 249, 528, 561, 574
MboII GAAGA 2 cut(s) 440, 637
MlsI TGGCCA 2 cut(s) 123, 751
MluCI AATT 2 cut(s) 77, 934
MluNI TGGCCA 2 cut(s) 123, 751
MlyI GAGTC 2 cut(s) 272, 662
MmeI TCCRAC 2 cut(s) 338, 848
Mox20I TGGCCA 2 cut(s) 123, 751
MroI TCCGGA 1 cut(s) 11
MscI TGGCCA 2 cut(s) 123, 751
MseI TTAA 2 cut(s) 383, 423
MslI CAYNNNNRTG 2 cut(s) 161, 584
Msp20I TGGCCA 2 cut(s) 123, 751
MspA1I CMGCKG 2 cut(s) 185, 203
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 694
MwoI GCNNNNNNNGC 3 cut(s) 194, 203, 361
NcoI CCATGG 1 cut(s) 156
NdeII GATC 5 cut(s) 72, 249, 528, 561, 574
NlaIII CATG 6 cut(s) 85, 133, 160, 211, 346, 486
NlaIV GGNNCC 1 cut(s) 139
NmuCI GTSAC 2 cut(s) 485, 654
NspI RCATGY 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 130
PkrI GCNGC 4 cut(s) 7, 187, 205, 718
PleI GAGTC 2 cut(s) 272, 661
PpsI GAGTC 2 cut(s) 272, 661
PpuMI RGGWCCY 1 cut(s) 907
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 3 cut(s) 215, 299, 907
PspPPI RGGWCCY 1 cut(s) 907
PstNI CAGNNNCTG 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 2 cut(s) 268, 847
RsaNI GTAC 2 cut(s) 267, 846
RseI CAYNNNNRTG 2 cut(s) 161, 584
SaqAI TTAA 2 cut(s) 383, 423
SatI GCNGC 4 cut(s) 6, 186, 204, 717
Sau3AI GATC 5 cut(s) 72, 249, 528, 561, 574
Sau96I GGNCC 3 cut(s) 215, 299, 907
ScaI AGTACT 2 cut(s) 268, 847
SchI GAGTC 2 cut(s) 272, 662
ScrFI CCNGG 1 cut(s) 213
SfaNI GCATC 2 cut(s) 15, 17
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 907
SmiMI CAYNNNNRTG 2 cut(s) 161, 584
Sse9I AATT 2 cut(s) 77, 934
SsiI CCGC 2 cut(s) 201, 716
SspMI CTAG 2 cut(s) 116, 594
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 4 cut(s) 257, 289, 497, 916
TaiI ACGT 1 cut(s) 868
TasI AATT 2 cut(s) 77, 934
TatI WGTACW 2 cut(s) 266, 845
TauI GCSGC 1 cut(s) 719
Tru1I TTAA 2 cut(s) 383, 423
Tru9I TTAA 2 cut(s) 383, 423
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 485, 654
TseI GCWGC 3 cut(s) 5, 185, 203
Tsp45I GTSAC 2 cut(s) 485, 654
TspDTI ATGAA 4 cut(s) 70, 331, 441, 564
TspGWI ACGGA 2 cut(s) 290, 905
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 907
XapI RAATTY 1 cut(s) 77
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 1 cut(s) 486
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 469
XspI CTAG 2 cut(s) 116, 594
ZrmI AGTACT 2 cut(s) 268, 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.