Rroxscaffold_2G00139280

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000171
Physical Location & Seq
Reverse (-)
77676033 .. 77677341
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139280.1

Sequence Viewer

Length: 864 bp
ATGAAAATGGAGAATATGAGAGTGAAGCATTTTGTGCCTTTTCTTTTGCTTCTTTGCTTGGCAAGAATCTGTACCAATATTGTTACAGCTGACCAATACTCATCGGGGAAGAAGCATTTTGTGCTGGTTCATGGAGCTAGTCATGGAGCGTGGTGTTGGTATAAGCTGGCAACTCTATTGAACTCCACAGGTCACAACGTTACAACTCTAGACCTTGCAGCATCTGGGATCAACCCGATCCAGGTACAGCAAGTTCATTCGTTCTCAGACTACGCTGAGCCATTGATCAAACTTCTGGGGTCTCTGTTACCAAAGGAGAGGGTTATCCTGGTCGGCCACAGCATGGGTGGGGCCGTGATATCAATTGCCATGGAGAGTTTCCCTGAAAAAATTGCTGCTGCTGTTTATGCCACTGCTGTTATGCCTGGCCCTGCTCTCGGCTACTTGACTATTTTTGAGAAGATTCGGGAAACAATATTGGCGTCGACTGACGTTCAGTATAGATATGATCAGGGGGCCAACAATCCTCCAACCTCGCTACTTTTTGAACCAAAACTATTGTCTTCGAGAGTGTACCAGCTATCGCCACCAGAGGATCTAAAACTAGCATTGTCATTGGTGAGATTTTCCCCTATATTTAATAAAGAAATAAAACTCACCGAGGAGAAGTACGGATTGGTTCCAAGAGTTTTCATCGTGTGTGACCAAGACCTTACAATAGAGGAGGATCTGCAAACGTGGATGATCAGGAAAAATCCCCCAAATGAAGTGAAGGTGATAAATGGTTCCGATCACATGGTCATGTTCTCTAGACCACTGGAGCTGTTCTCCAACCTCCTGAACGTTGCTGAGAAATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

287

Amino Acids

32.09

Weight (kDa)

7.09

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 37 - 160 2.5e-11 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 39 - 272 1.4e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 40 - 276 2.8e-23 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 343
AccI GTMKAC 1 cut(s) 485
AclI AACGTT 2 cut(s) 198, 843
AclWI GGATC 4 cut(s) 232, 236, 603, 735
AcoI YGGCCR 1 cut(s) 334
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 4 cut(s) 73, 246, 575, 671
AfiI CCNNNNNNNGG 3 cut(s) 241, 343, 437
AgsI TTSAA 2 cut(s) 181, 548
AjnI CCWGG 3 cut(s) 240, 327, 424
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AluBI AGCT 5 cut(s) 89, 137, 166, 580, 823
AluI AGCT 5 cut(s) 89, 137, 166, 580, 823
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 4 cut(s) 232, 236, 603, 735
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 4 cut(s) 334, 351, 427, 516
ApeKI GCWGC 3 cut(s) 218, 395, 398
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AspS9I GGNCC 3 cut(s) 351, 428, 516
AsuHPI GGTGA 3 cut(s) 631, 649, 787
BbsI GAAGAC 1 cut(s) 555
BbvI GCAGC 3 cut(s) 230, 382, 385
BceAI ACGGC 1 cut(s) 338
BciT130I CCWGG 3 cut(s) 242, 329, 426
BclI TGATCA 3 cut(s) 285, 508, 744
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 4 cut(s) 138, 209, 605, 810
BisI GCNGC 3 cut(s) 219, 396, 399
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 3 cut(s) 220, 397, 400
Bme1390I CCNGG 3 cut(s) 242, 329, 426
BmgT120I GGNCC 3 cut(s) 351, 428, 516
BmiI GGNNCC 4 cut(s) 352, 517, 681, 787
BmrFI CCNGG 3 cut(s) 242, 329, 426
BmsI GCATC 1 cut(s) 230
BpiI GAAGAC 1 cut(s) 555
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 1 cut(s) 839
Bpu1102I GCTNAGC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 482
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 369, 660
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 343, 437
Bse1I ACTGG 1 cut(s) 822
BseBI CCWGG 3 cut(s) 242, 329, 426
BseDI CCNNGG 2 cut(s) 369, 660
BseGI GGATG 1 cut(s) 747
BseLI CCNNNNNNNGG 3 cut(s) 241, 343, 437
BseMII CTCAG 3 cut(s) 267, 279, 840
BseNI ACTGG 1 cut(s) 822
BseRI GAGGAG 2 cut(s) 677, 737
BseXI GCAGC 3 cut(s) 230, 382, 385
BshFI GGCC 4 cut(s) 336, 353, 429, 518
BslI CCNNNNNNNGG 3 cut(s) 241, 343, 437
BsmAI GTCTC 1 cut(s) 306
BsnI GGCC 4 cut(s) 336, 353, 429, 518
Bso31I GGTCTC 1 cut(s) 306
Bsp143I GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
Bsp1720I GCTNAGC 1 cut(s) 276
Bsp19I CCATGG 1 cut(s) 369
BspANI GGCC 4 cut(s) 336, 353, 429, 518
BspCNI CTCAG 3 cut(s) 268, 278, 841
BspLI GGNNCC 4 cut(s) 352, 517, 681, 787
BspPI GGATC 4 cut(s) 232, 236, 603, 735
BspTNI GGTCTC 1 cut(s) 306
BsrI ACTGG 1 cut(s) 822
BssECI CCNNGG 2 cut(s) 369, 660
BssMI GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
BssNI GRCGYC 1 cut(s) 482
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 3 cut(s) 242, 329, 426
BstACI GRCGYC 1 cut(s) 482
BstAPI GCANNNNNTGC 2 cut(s) 34, 121
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 3 cut(s) 265, 276, 849
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 747
BstKTI GATC 8 cut(s) 231, 240, 288, 511, 598, 730, 747, 793
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
BstMWI GCNNNNNNNGC 3 cut(s) 34, 121, 407
BstNI CCWGG 3 cut(s) 242, 329, 426
BstSCI CCNGG 3 cut(s) 240, 327, 424
BstV1I GCAGC 3 cut(s) 230, 382, 385
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 2 cut(s) 595, 727
BstYI RGATCY 2 cut(s) 595, 727
BsuRI GGCC 4 cut(s) 336, 353, 429, 518
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 747
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 411, 815
Cac8I GCNNGC 1 cut(s) 168
CaiI CAGNNNCTG 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 351, 428, 516
CseI GACGC 1 cut(s) 471
Csp6I GTAC 4 cut(s) 72, 245, 574, 670
CviAII CATG 6 cut(s) 131, 143, 343, 370, 796, 802
CviQI GTAC 4 cut(s) 72, 245, 574, 670
DdeI CTNAG 3 cut(s) 265, 276, 849
DpnI GATC 8 cut(s) 230, 239, 287, 510, 597, 729, 746, 792
DpnII GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
EaeI YGGCCR 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 360
EcoRII CCWGG 3 cut(s) 240, 327, 424
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 6 cut(s) 134, 146, 346, 373, 799, 805
FatI CATG 6 cut(s) 130, 142, 342, 369, 795, 801
FbaI TGATCA 3 cut(s) 285, 508, 744
FblI GTMKAC 1 cut(s) 485
Fnu4HI GCNGC 3 cut(s) 219, 396, 399
FokI GGATG 1 cut(s) 754
Fsp4HI GCNGC 3 cut(s) 219, 396, 399
FspBI CTAG 4 cut(s) 138, 209, 605, 810
GluI GCNGC 3 cut(s) 219, 396, 399
GsuI CTGGAG 1 cut(s) 839
HaeIII GGCC 4 cut(s) 336, 353, 429, 518
HgaI GACGC 1 cut(s) 471
Hin1I GRCGYC 1 cut(s) 482
Hin1II CATG 6 cut(s) 134, 146, 346, 373, 799, 805
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 2 cut(s) 66, 463
HphI GGTGA 3 cut(s) 631, 649, 787
Hpy166II GTNNAC 2 cut(s) 486, 574
Hpy188I TCNGA 2 cut(s) 268, 790
Hpy188III TCNNGA 6 cut(s) 209, 467, 567, 748, 810, 838
Hpy8I GTNNAC 2 cut(s) 486, 574
Hpy99I CGWCG 1 cut(s) 487
HpyAV CCTTC 1 cut(s) 766
HpyCH4IV ACGT 4 cut(s) 198, 492, 737, 843
HpyCH4V TGCA 2 cut(s) 218, 733
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 121, 407
HpyF3I CTNAG 3 cut(s) 265, 276, 849
HpySE526I ACGT 4 cut(s) 198, 492, 737, 843
Hsp92I GRCGYC 1 cut(s) 482
Hsp92II CATG 6 cut(s) 134, 146, 346, 373, 799, 805
Ksp22I TGATCA 3 cut(s) 285, 508, 744
Kzo9I GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
LmnI GCTCC 3 cut(s) 134, 146, 820
Lsp1109I GCAGC 3 cut(s) 230, 382, 385
LweI GCATC 1 cut(s) 230
MaeI CTAG 4 cut(s) 138, 209, 605, 810
MaeII ACGT 4 cut(s) 198, 492, 737, 843
MaeIII GTNAC 5 cut(s) 82, 191, 199, 306, 701
MalI GATC 8 cut(s) 230, 239, 287, 510, 597, 729, 746, 792
MboI GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
MboII GAAGA 3 cut(s) 121, 472, 555
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 2 cut(s) 595, 727
MluCI AATT 2 cut(s) 363, 390
MmeI TCCRAC 2 cut(s) 554, 855
MnlI CCTC 8 cut(s) 312, 537, 544, 586, 655, 715, 718, 845
MseI TTAA 2 cut(s) 639, 862
MslI CAYNNNNRTG 1 cut(s) 800
MspA1I CMGCKG 1 cut(s) 89
MspR9I CCNGG 3 cut(s) 242, 329, 426
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 3 cut(s) 242, 329, 426
MwoI GCNNNNNNNGC 3 cut(s) 34, 121, 407
NcoI CCATGG 1 cut(s) 369
NdeII GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
NlaIII CATG 6 cut(s) 134, 146, 346, 373, 799, 805
NlaIV GGNNCC 4 cut(s) 352, 517, 681, 787
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 2 cut(s) 191, 701
PfeI GAWTC 2 cut(s) 66, 463
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 3 cut(s) 220, 397, 400
Psp1406I AACGTT 2 cut(s) 198, 843
Psp6I CCWGG 3 cut(s) 240, 327, 424
PspGI CCWGG 3 cut(s) 240, 327, 424
PspN4I GGNNCC 4 cut(s) 352, 517, 681, 787
PspPI GGNCC 3 cut(s) 351, 428, 516
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 2 cut(s) 595, 727
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 4 cut(s) 73, 246, 575, 671
RsaNI GTAC 4 cut(s) 72, 245, 574, 670
RseI CAYNNNNRTG 1 cut(s) 800
SalI GTCGAC 1 cut(s) 484
SaqAI TTAA 2 cut(s) 639, 862
SatI GCNGC 3 cut(s) 219, 396, 399
Sau3AI GATC 8 cut(s) 228, 237, 285, 508, 595, 727, 744, 790
Sau96I GGNCC 3 cut(s) 351, 428, 516
ScrFI CCNGG 3 cut(s) 242, 329, 426
SfaNI GCATC 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 800
Sse9I AATT 2 cut(s) 363, 390
SspI AATATT 3 cut(s) 79, 477, 857
SspMI CTAG 4 cut(s) 138, 209, 605, 810
StyD4I CCNGG 3 cut(s) 240, 327, 424
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 4 cut(s) 201, 495, 740, 846
TaqI TCGA 2 cut(s) 485, 566
TasI AATT 2 cut(s) 363, 390
TfiI GAWTC 2 cut(s) 66, 463
Tru1I TTAA 2 cut(s) 639, 862
Tru9I TTAA 2 cut(s) 639, 862
TscAI CASTG 2 cut(s) 418, 822
TseFI GTSAC 2 cut(s) 191, 701
TseI GCWGC 3 cut(s) 218, 395, 398
Tsp45I GTSAC 2 cut(s) 191, 701
TspDTI ATGAA 5 cut(s) 17, 119, 245, 682, 780
TspGWI ACGGA 1 cut(s) 687
TspRI CASTG 2 cut(s) 418, 822
Van91I CCANNNNNTGG 1 cut(s) 343
XbaI TCTAGA 2 cut(s) 208, 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 485
XspI CTAG 4 cut(s) 138, 209, 605, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.