MD02G1161800.v1.1

Salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000171
Physical Location & Seq
Forward (+)
13607749 .. 13617277
9529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1161800.v1.1.491

Sequence Viewer

Length: 861 bp
ATGGAAAACATGACAGAGAAGCTCCTTTTGTTTTACCTTTTATTTATGTGCGTAGCAAAAGCTAGTACCTCAACCCCGTTCCCAATTCACAACCAAACTCAAAGTCCAAAACATTTTGTGCTTATACATGGTGCTTGTTATGGAGCATGGAGTTGGTATAAGGTGGCGACTCTCCTGAAGAACTCAGGTCACAATGTCACAGCTCTAGACTTGGGAGCATCCGGGATCAACCCGATCCAGGTAGAGCAACTCCCTTCGTTATTGGAATACGTCGACCCTTTGACTAAGGTCATGGTGTCTCTACCACCAAATGAAGAGGTTATCCTTGTGGCTCATAGCTTGGGTGGAGCCGTCATATCTATTTTCATGGAGAGGTTCCCTCAGAAAATTGCTGCTGCGGTGTATGTTACGGCTGTCATGTCTGGTCCTACTCTCAATTACTCAACTATATTTGCAAAGGTTACCAAAAGATTTGATTTTATGGACTCTCAATTTAGATATGATAACGGGACCAACAACCCTGCAACCTCCCTTCTCTTTGGGCCTAAGGTATTGGCGACAAGTTTTTTGCAGCTCTCACCACAACAGGATTTAACTCTAGCGTTATCGTTGGTGAGATTTTCTCCTTTATATAATTATGATCTAATAAAACTCACGGAGGAGAATTATGGATCAGTTCGTAGAGTATTCATTGTGTCCGCCCAAGACCGTGCGATAGTGTTGGATGTGCAAAATTACATGATCAATAACAATCCCCCAAATGAAGTGAAGGTGATAATAGGTTCTGATCACTTGGTCATGCTCTCTAAACCTGTGGAGTTGTTCTTTCATCTCCAAAATATTGCTGAGAAATATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

287

Amino Acids

31.94

Weight (kDa)

6.65

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 38 - 271 3.2e-17 alpha/beta hydrolase fold
Hydrolase_4 PF12146 38 - 151 2.6e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 39 - 275 2.1e-18 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 273
AciI CCGC 2 cut(s) 398, 699
AclWI GGATC 3 cut(s) 229, 233, 679
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 238
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 5 cut(s) 22, 62, 203, 339, 574
AluI AGCT 5 cut(s) 22, 62, 203, 339, 574
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 3 cut(s) 229, 233, 679
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 3 cut(s) 392, 395, 571
ArsI GACNNNNNNTTYG 4 cut(s) 88, 120, 550, 582
AspS9I GGNCC 3 cut(s) 425, 510, 542
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 570, 625, 784
AvaII GGWCC 2 cut(s) 425, 510
AxyI CCTNAGG 1 cut(s) 546
BbvI GCAGC 3 cut(s) 379, 382, 583
BceAI ACGGC 2 cut(s) 335, 426
BciT130I CCWGG 1 cut(s) 239
BclI TGATCA 2 cut(s) 741, 787
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 3 cut(s) 63, 206, 599
BisI GCNGC 3 cut(s) 393, 396, 572
BlsI GCNGC 3 cut(s) 394, 397, 573
Bme1390I CCNGG 2 cut(s) 223, 239
Bme18I GGWCC 2 cut(s) 425, 510
BmgT120I GGNCC 3 cut(s) 425, 510, 542
BmiI GGNNCC 3 cut(s) 349, 377, 511
BmrFI CCNGG 2 cut(s) 223, 239
BmsI GCATC 1 cut(s) 227
BoxI GACNNNNGTC 1 cut(s) 287
BplI GAGNNNNNCTC 4 cut(s) 364, 396, 607, 639
BpuMI CCSGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse21I CCTNAGG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 239
BseGI GGATG 2 cut(s) 218, 730
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 3 cut(s) 198, 395, 837
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 3 cut(s) 379, 382, 583
BshFI GGCC 1 cut(s) 544
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 1 cut(s) 544
Bsp143I GATC 6 cut(s) 225, 234, 640, 671, 741, 787
BspACI CCGC 2 cut(s) 398, 699
BspANI GGCC 1 cut(s) 544
BspCNI CTCAG 3 cut(s) 197, 394, 838
BspLI GGNNCC 3 cut(s) 349, 377, 511
BspPI GGATC 3 cut(s) 229, 233, 679
BssMI GATC 6 cut(s) 225, 234, 640, 671, 741, 787
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 1 cut(s) 710
Bst6I CTCTTC 1 cut(s) 309
BstDEI CTNAG 5 cut(s) 184, 285, 381, 546, 846
BstEII GGTNACC 1 cut(s) 460
BstF5I GGATG 2 cut(s) 218, 730
BstKTI GATC 6 cut(s) 228, 237, 643, 674, 744, 790
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 6 cut(s) 225, 234, 640, 671, 741, 787
BstNI CCWGG 1 cut(s) 239
BstPAI GACNNNNGTC 1 cut(s) 287
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 2 cut(s) 221, 237
BstV1I GCAGC 3 cut(s) 379, 382, 583
Bsu36I CCTNAGG 1 cut(s) 546
BsuRI GGCC 1 cut(s) 544
BtsCI GGATG 2 cut(s) 218, 730
Cfr13I GGNCC 3 cut(s) 425, 510, 542
Csp6I GTAC 1 cut(s) 66
CviAII CATG 8 cut(s) 10, 128, 147, 292, 367, 418, 739, 799
CviJI RGCY 9 cut(s) 22, 62, 203, 332, 339, 350, 413, 544, 574
CviKI_1 RGCY 9 cut(s) 22, 62, 203, 332, 339, 350, 413, 544, 574
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 5 cut(s) 184, 285, 381, 546, 846
DpnI GATC 6 cut(s) 227, 236, 642, 673, 743, 789
DpnII GATC 6 cut(s) 225, 234, 640, 671, 741, 787
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 425, 510
Eco57I CTGAAG 1 cut(s) 197
Eco81I CCTNAGG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 460
EcoRII CCWGG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 859
FaeI CATG 8 cut(s) 13, 131, 150, 295, 370, 421, 742, 802
FaqI GGGAC 1 cut(s) 523
FatI CATG 8 cut(s) 9, 127, 146, 291, 366, 417, 738, 798
FbaI TGATCA 2 cut(s) 741, 787
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 393, 396, 572
FokI GGATG 2 cut(s) 205, 737
Fsp4HI GCNGC 3 cut(s) 393, 396, 572
FspBI CTAG 3 cut(s) 63, 206, 599
GluI GCNGC 3 cut(s) 393, 396, 572
HaeIII GGCC 1 cut(s) 544
HapII CCGG 1 cut(s) 222
Hin1II CATG 8 cut(s) 13, 131, 150, 295, 370, 421, 742, 802
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 2 cut(s) 169, 485
HpaII CCGG 1 cut(s) 222
HphI GGTGA 3 cut(s) 570, 625, 784
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 2 cut(s) 384, 787
Hpy188III TCNNGA 2 cut(s) 175, 206
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 264, 542, 763
HpyCH4III ACNGT 1 cut(s) 710
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 5 cut(s) 455, 524, 571, 730, 857
HpyF3I CTNAG 5 cut(s) 184, 285, 381, 546, 846
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 8 cut(s) 13, 131, 150, 295, 370, 421, 742, 802
Ksp22I TGATCA 2 cut(s) 741, 787
Kzo9I GATC 6 cut(s) 225, 234, 640, 671, 741, 787
LmnI GCTCC 4 cut(s) 27, 143, 215, 347
LpnPI CCDG 9 cut(s) 171, 188, 224, 235, 251, 408, 534, 572, 825
Lsp1109I GCAGC 3 cut(s) 379, 382, 583
LweI GCATC 1 cut(s) 227
MaeI CTAG 3 cut(s) 63, 206, 599
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 4 cut(s) 188, 196, 406, 460
MalI GATC 6 cut(s) 227, 236, 642, 673, 743, 789
MboI GATC 6 cut(s) 225, 234, 640, 671, 741, 787
MboII GAAGA 2 cut(s) 190, 326
MluCI AATT 7 cut(s) 84, 387, 436, 491, 634, 664, 733
MlyI GAGTC 2 cut(s) 163, 479
MmeI TCCRAC 1 cut(s) 702
MnlI CCTC 6 cut(s) 79, 310, 366, 390, 538, 652
Mph1103I ATGCAT 1 cut(s) 859
MseI TTAA 1 cut(s) 593
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 223, 239
MvaI CCWGG 1 cut(s) 239
NciI CCSGG 1 cut(s) 223
NdeII GATC 6 cut(s) 225, 234, 640, 671, 741, 787
NlaIII CATG 8 cut(s) 13, 131, 150, 295, 370, 421, 742, 802
NlaIV GGNNCC 3 cut(s) 349, 377, 511
NmuCI GTSAC 2 cut(s) 188, 196
NsiI ATGCAT 1 cut(s) 859
PfoI TCCNGGA 1 cut(s) 221
PkrI GCNGC 3 cut(s) 394, 397, 573
PleI GAGTC 2 cut(s) 163, 479
PpsI GAGTC 2 cut(s) 163, 479
PshAI GACNNNNGTC 1 cut(s) 287
Psp6I CCWGG 1 cut(s) 237
PspEI GGTNACC 1 cut(s) 460
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 349, 377, 511
PspPI GGNCC 3 cut(s) 425, 510, 542
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SalI GTCGAC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 593
SatI GCNGC 3 cut(s) 393, 396, 572
Sau3AI GATC 6 cut(s) 225, 234, 640, 671, 741, 787
Sau96I GGNCC 3 cut(s) 425, 510, 542
SchI GAGTC 2 cut(s) 163, 479
ScrFI CCNGG 2 cut(s) 223, 239
SfaNI GCATC 1 cut(s) 227
SinI GGWCC 2 cut(s) 425, 510
Sse9I AATT 7 cut(s) 84, 387, 436, 491, 634, 664, 733
SsiI CCGC 2 cut(s) 398, 699
SspI AATATT 1 cut(s) 841
SspMI CTAG 3 cut(s) 63, 206, 599
StyD4I CCNGG 2 cut(s) 221, 237
TaaI ACNGT 1 cut(s) 710
TaiI ACGT 1 cut(s) 273
TaqI TCGA 1 cut(s) 273
TasI AATT 7 cut(s) 84, 387, 436, 491, 634, 664, 733
Tru1I TTAA 1 cut(s) 593
Tru9I TTAA 1 cut(s) 593
TseFI GTSAC 2 cut(s) 188, 196
TseI GCWGC 3 cut(s) 392, 395, 571
Tsp45I GTSAC 2 cut(s) 188, 196
TspDTI ATGAA 5 cut(s) 327, 355, 679, 777, 818
TspGWI ACGGA 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 425, 510
XbaI TCTAGA 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 3 cut(s) 63, 206, 599
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.