Rh1BG081700

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000171
Physical Location & Seq
Forward (+)
12903956 .. 12904531
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG081700.1

Sequence Viewer

Length: 576 bp
ATGGTAAACGCATCAAGCCAAGAAATGAAACCGGAGAAAATGAGAGCAAAGCATTTTGTGCCCGTTCTTTTGTTGTTTTTTTATATTAGCTTGGCAACAATCTGTGCCTCAAGAACACCTCAGAAACCGAACCAAAACCCAAATAAGATGAAGCATTTCGTGCTTGTTCACGGTGCTGGTCACGGAGCATGGTGCTGGTACAAGCTAACAACCCTACCGACCACCGCTGGTCACAACGTCACAACCCTAGACCTTCCTGGATCAGGCATAGACCCAATGCAGGTACAACAAATCCATTCCATCGCAGACTATTCTGAGCCCTTGATCAAGCTCATGGAGTCTCTGCCACCGCATGATAGGGTTATCCTAGCAGGGCACAGCTTGGGTGGGCTCCCGATATCTCTTCTCATGGAGAGGTTCCCTAGAAAAATTGCTGCTGCAGTTTTTGCCACCGCTAGCATGCCTGGTCTAAATATCAGTTTGGCCACTATATCTGAAGAGATAAATAGTTCTACACATGCATATGTTTTTGGAGTACAGACACTACAGGGTGATATTAAGAAAAAGTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

191

Amino Acids

21.02

Weight (kDa)

9.27

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 51 - 152 4.7e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 52 - 154 3.6e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 53 - 155 3.2e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AciI CCGC 3 cut(s) 225, 350, 453
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 483
AcuI CTGAAG 1 cut(s) 516
AfaI GTAC 3 cut(s) 200, 285, 537
AfiI CCNNNNNNNGG 2 cut(s) 263, 280
AjnI CCWGG 2 cut(s) 256, 463
AluBI AGCT 4 cut(s) 90, 205, 331, 381
AluI AGCT 4 cut(s) 90, 205, 331, 381
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 1 cut(s) 268
AoxI GGCC 1 cut(s) 483
ApeKI GCWGC 2 cut(s) 434, 437
Asp700I GAANNNNTTC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 563
AsuNHI GCTAGC 1 cut(s) 455
BaeGI GKGCMC 2 cut(s) 63, 378
BalI TGGCCA 1 cut(s) 485
BanII GRGCYC 2 cut(s) 321, 393
BbvI GCAGC 2 cut(s) 421, 424
BccI CCATC 1 cut(s) 308
BciT130I CCWGG 2 cut(s) 258, 465
BclI TGATCA 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 4 cut(s) 248, 368, 423, 456
BfmI CTRYAG 2 cut(s) 438, 545
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 2 cut(s) 435, 438
BlsI GCNGC 2 cut(s) 436, 439
Bme1390I CCNGG 2 cut(s) 258, 465
BmiI GGNNCC 2 cut(s) 392, 419
BmrFI CCNGG 2 cut(s) 258, 465
BmsI GCATC 1 cut(s) 20
BmtI GCTAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 94
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 280
BseBI CCWGG 2 cut(s) 258, 465
BseLI CCNNNNNNNGG 2 cut(s) 263, 280
BseMII CTCAG 2 cut(s) 134, 306
BseSI GKGCMC 2 cut(s) 63, 378
BseXI GCAGC 2 cut(s) 421, 424
BshFI GGCC 1 cut(s) 485
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 2 cut(s) 263, 280
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 1 cut(s) 485
Bsp1286I GDGCHC 4 cut(s) 63, 321, 378, 393
Bsp143I GATC 2 cut(s) 260, 324
BspACI CCGC 3 cut(s) 225, 350, 453
BspANI GGCC 1 cut(s) 485
BspCNI CTCAG 2 cut(s) 133, 307
BspLI GGNNCC 2 cut(s) 392, 419
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 1 cut(s) 271
BspOI GCTAGC 1 cut(s) 459
BspPI GGATC 1 cut(s) 268
BssMI GATC 2 cut(s) 260, 324
Bst2UI CCWGG 2 cut(s) 258, 465
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 2 cut(s) 408, 492
BstAPI GCANNNNNTGC 3 cut(s) 58, 160, 446
BstC8I GCNNGC 2 cut(s) 457, 461
BstDEI CTNAG 2 cut(s) 120, 315
BstENI CCTNNNNNAGG 1 cut(s) 261
BstKTI GATC 2 cut(s) 263, 327
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 260, 324
BstMWI GCNNNNNNNGC 3 cut(s) 58, 160, 446
BstNI CCWGG 2 cut(s) 258, 465
BstNSI RCATGY 2 cut(s) 463, 521
BstSCI CCNGG 2 cut(s) 256, 463
BstSFI CTRYAG 2 cut(s) 438, 545
BstSLI GKGCMC 2 cut(s) 63, 378
BstV1I GCAGC 2 cut(s) 421, 424
BsuRI GGCC 1 cut(s) 485
BtgZI GCGATG 1 cut(s) 286
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 2 cut(s) 457, 461
Csp6I GTAC 3 cut(s) 199, 284, 536
CviAII CATG 6 cut(s) 189, 334, 353, 409, 460, 518
CviJI RGCY 8 cut(s) 18, 90, 205, 319, 331, 381, 391, 485
CviKI_1 RGCY 8 cut(s) 18, 90, 205, 319, 331, 381, 391, 485
CviQI GTAC 3 cut(s) 199, 284, 536
DdeI CTNAG 2 cut(s) 120, 315
DpnI GATC 2 cut(s) 262, 326
DpnII GATC 2 cut(s) 260, 324
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 408, 492
EarI CTCTTC 2 cut(s) 408, 492
Eco24I GRGCYC 2 cut(s) 321, 393
Eco32I GATATC 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 516
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 256, 463
EcoRV GATATC 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 523
EcoT38I GRGCYC 2 cut(s) 321, 393
FaeI CATG 6 cut(s) 192, 337, 356, 412, 463, 521
FatI CATG 6 cut(s) 188, 333, 352, 408, 459, 517
FauNDI CATATG 1 cut(s) 523
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 2 cut(s) 435, 438
FriOI GRGCYC 2 cut(s) 321, 393
Fsp4HI GCNGC 2 cut(s) 435, 438
FspBI CTAG 4 cut(s) 248, 368, 423, 456
GluI GCNGC 2 cut(s) 435, 438
HaeIII GGCC 1 cut(s) 485
HapII CCGG 1 cut(s) 32
Hin1II CATG 6 cut(s) 192, 337, 356, 412, 463, 521
HinfI GANTC 1 cut(s) 338
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 7, 169
Hpy188I TCNGA 3 cut(s) 123, 316, 496
Hpy188III TCNNGA 2 cut(s) 111, 394
Hpy8I GTNNAC 2 cut(s) 7, 169
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 3 cut(s) 280, 440, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 160, 446
HpyF3I CTNAG 2 cut(s) 120, 315
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 6 cut(s) 192, 337, 356, 412, 463, 521
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 2 cut(s) 260, 324
LmnI GCTCC 2 cut(s) 185, 396
Lsp1109I GCAGC 2 cut(s) 421, 424
LweI GCATC 1 cut(s) 20
MaeI CTAG 4 cut(s) 248, 368, 423, 456
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 3 cut(s) 179, 230, 238
MalI GATC 2 cut(s) 262, 326
MboI GATC 2 cut(s) 260, 324
MboII GAAGA 2 cut(s) 395, 509
MhlI GDGCHC 4 cut(s) 63, 321, 378, 393
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 1 cut(s) 429
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 1 cut(s) 347
MnlI CCTC 3 cut(s) 118, 129, 408
Mox20I TGGCCA 1 cut(s) 485
Mph1103I ATGCAT 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 155
MscI TGGCCA 1 cut(s) 485
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 258, 465
MvaI CCWGG 2 cut(s) 258, 465
MwoI GCNNNNNNNGC 3 cut(s) 58, 160, 446
NdeI CATATG 1 cut(s) 523
NdeII GATC 2 cut(s) 260, 324
NheI GCTAGC 1 cut(s) 455
NlaIII CATG 6 cut(s) 192, 337, 356, 412, 463, 521
NlaIV GGNNCC 2 cut(s) 392, 419
NmuCI GTSAC 3 cut(s) 179, 230, 238
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 2 cut(s) 463, 521
PaeI GCATGC 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 256
PkrI GCNGC 2 cut(s) 436, 439
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 256, 463
PspGI CCWGG 2 cut(s) 256, 463
PspN4I GGNNCC 2 cut(s) 392, 419
PstI CTGCAG 1 cut(s) 442
RsaI GTAC 3 cut(s) 200, 285, 537
RsaNI GTAC 3 cut(s) 199, 284, 536
RseI CAYNNNNRTG 1 cut(s) 522
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 2 cut(s) 435, 438
Sau3AI GATC 2 cut(s) 260, 324
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 2 cut(s) 258, 465
SduI GDGCHC 4 cut(s) 63, 321, 378, 393
SetI ASST 9 cut(s) 92, 121, 207, 240, 255, 285, 333, 383, 419
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 2 cut(s) 438, 545
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
SphI GCATGC 1 cut(s) 463
Sse9I AATT 1 cut(s) 429
SsiI CCGC 3 cut(s) 225, 350, 453
SspMI CTAG 4 cut(s) 248, 368, 423, 456
StyD4I CCNGG 2 cut(s) 256, 463
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 1 cut(s) 240
TasI AATT 1 cut(s) 429
TatI WGTACW 1 cut(s) 535
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseFI GTSAC 3 cut(s) 179, 230, 238
TseI GCWGC 2 cut(s) 434, 437
Tsp45I GTSAC 3 cut(s) 179, 230, 238
TspDTI ATGAA 2 cut(s) 41, 164
TspGWI ACGGA 1 cut(s) 198
XagI CCTNNNNNAGG 1 cut(s) 261
XceI RCATGY 2 cut(s) 463, 521
XmnI GAANNNNTTC 1 cut(s) 155
XspI CTAG 4 cut(s) 248, 368, 423, 456
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.