Rroxscaffold_2G00139320

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000171
Physical Location & Seq
Reverse (-)
77751588 .. 77753037
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139320.1

Sequence Viewer

Length: 882 bp
ATGCAGGCTTACGAAAGACTGGTGAAAATGGAGAATATAAGAGTGAAGCATTTTGTGCCTTTTCTTTTGCTTCTTTGCTTGGCAAGAATCTGTACTAATATTGTTGAAGCTGACCAAATCTCATCTGGGAAGAAGCATTTTGTGCTGGTTCATGGAGCTGGTGGCGGAGCTTGGTGTTGGTATAAGCTGGCAACTCTATTGAACTCTACAGGTCACAATGTTACAACTATAGACCTTGCAGCATCCGGTATCAACCCGATCCAGGTACAGCAAGTTCATTCGTTCTCAGACTACGTTGAGCCATTGATCAAATTTCTGGGGTCTCTGTTACCAAAAGAGAGGGTTATCCTGGTTGGCCACAGCATGGGTGGGGCCGTGATATCAATTGCCATGGAGAGGTTCCCTGAGAAAATTGCTGCAGCTGTTTATGCCACTGCTTTCATGCCTGGTCCTGCTCTGAGTTACTTAACTATTTTTGAGAAGATTCAGGAAAGATCGGTGTTGACTGACACTCAGTATAGATATGATCAGGGGGCCAACAATCCTCCAACCTCGCTACTCTTTGGACCAAAATATTTGTCTTCGAGAGTGTACCAGCTATCGCCACCAGAGGATCTAGAACTAGCATTGTCATTGGAGAGATTTTCCCCTCTATTTATTGAAGAAATAAAACTCACTGAGGAGAAGTACGGATTGGTTCCAAGAGTTTTCATCGTGTGTGACCAAGACCTTTCAATAGAGGAGGATCTGCAAACGTGGATGATCAGGAAAAATCCCCCAAATGAAGTGAAGGTGATAAATGGTTCCGATCACATGGTCATGTTCTCTAGACCATTGGAGCTGTTCTCCAACCTCCTGAAGGTTGCTGAGAAATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

293

Amino Acids

32.97

Weight (kDa)

6.19

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 44 - 149 4.1e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 46 - 278 4.5e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 47 - 282 3.6e-23 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 364
AciI CCGC 1 cut(s) 165
AclWI GGATC 3 cut(s) 253, 621, 753
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 878
AfaI GTAC 4 cut(s) 94, 267, 593, 689
AfiI CCNNNNNNNGG 4 cut(s) 262, 364, 396, 859
AgsI TTSAA 4 cut(s) 107, 202, 662, 735
AjnI CCWGG 3 cut(s) 261, 348, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AluBI AGCT 7 cut(s) 110, 158, 170, 187, 422, 598, 841
AluI AGCT 7 cut(s) 110, 158, 170, 187, 422, 598, 841
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 3 cut(s) 253, 621, 753
AoxI GGCC 3 cut(s) 355, 372, 534
ApeKI GCWGC 3 cut(s) 239, 416, 419
ApoI RAATTY 1 cut(s) 311
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
AspS9I GGNCC 4 cut(s) 372, 449, 534, 566
AsuHPI GGTGA 2 cut(s) 34, 805
AvaII GGWCC 2 cut(s) 449, 566
BalI TGGCCA 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 573
BbvI GCAGC 3 cut(s) 251, 403, 431
BceAI ACGGC 1 cut(s) 359
BciT130I CCWGG 3 cut(s) 263, 350, 447
BclI TGATCA 3 cut(s) 306, 526, 762
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 3 cut(s) 617, 623, 828
BfmI CTRYAG 3 cut(s) 207, 228, 417
BisI GCNGC 3 cut(s) 240, 417, 420
BlsI GCNGC 3 cut(s) 241, 418, 421
Bme1390I CCNGG 3 cut(s) 263, 350, 447
Bme18I GGWCC 2 cut(s) 449, 566
BmgT120I GGNCC 4 cut(s) 372, 449, 534, 566
BmiI GGNNCC 5 cut(s) 373, 401, 535, 699, 805
BmrFI CCNGG 3 cut(s) 263, 350, 447
BmsI GCATC 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 573
BplI GAGNNNNNCTC 2 cut(s) 830, 862
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 390
BsaWI WCCGGW 1 cut(s) 245
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 364, 396, 859
Bse1I ACTGG 1 cut(s) 24
BseBI CCWGG 3 cut(s) 263, 350, 447
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 2 cut(s) 242, 765
BseLI CCNNNNNNNGG 4 cut(s) 262, 364, 396, 859
BseMII CTCAG 6 cut(s) 300, 396, 449, 527, 669, 858
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 2 cut(s) 695, 755
BseXI GCAGC 3 cut(s) 251, 403, 431
BshFI GGCC 3 cut(s) 357, 374, 536
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 4 cut(s) 262, 364, 396, 859
BsmAI GTCTC 1 cut(s) 327
BsnI GGCC 3 cut(s) 357, 374, 536
Bso31I GGTCTC 1 cut(s) 327
Bsp143I GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 1 cut(s) 165
BspANI GGCC 3 cut(s) 357, 374, 536
BspCNI CTCAG 6 cut(s) 299, 397, 450, 526, 670, 859
BspLI GGNNCC 5 cut(s) 373, 401, 535, 699, 805
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 3 cut(s) 253, 621, 753
BspTNI GGTCTC 1 cut(s) 327
BsrI ACTGG 1 cut(s) 24
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 3 cut(s) 263, 350, 447
BstAPI GCANNNNNTGC 2 cut(s) 55, 142
BstC8I GCNNGC 2 cut(s) 6, 189
BstDEI CTNAG 6 cut(s) 286, 405, 458, 513, 678, 867
BstDSI CCRYGG 1 cut(s) 390
BstENI CCTNNNNNAGG 1 cut(s) 857
BstF5I GGATG 2 cut(s) 242, 765
BstKTI GATC 8 cut(s) 261, 309, 497, 529, 616, 748, 765, 811
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
BstMWI GCNNNNNNNGC 3 cut(s) 55, 142, 428
BstNI CCWGG 3 cut(s) 263, 350, 447
BstSCI CCNGG 3 cut(s) 261, 348, 445
BstSFI CTRYAG 3 cut(s) 207, 228, 417
BstV1I GCAGC 3 cut(s) 251, 403, 431
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 2 cut(s) 613, 745
BstYI RGATCY 2 cut(s) 613, 745
BsuRI GGCC 3 cut(s) 357, 374, 536
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 2 cut(s) 242, 765
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 432, 675
Cac8I GCNNGC 2 cut(s) 6, 189
Cfr13I GGNCC 4 cut(s) 372, 449, 534, 566
Csp6I GTAC 4 cut(s) 93, 266, 592, 688
CviAII CATG 6 cut(s) 152, 364, 391, 442, 814, 820
CviQI GTAC 4 cut(s) 93, 266, 592, 688
DdeI CTNAG 6 cut(s) 286, 405, 458, 513, 678, 867
DpnI GATC 8 cut(s) 260, 308, 496, 528, 615, 747, 764, 810
DpnII GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
EaeI YGGCCR 1 cut(s) 355
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 381
Eco47I GGWCC 2 cut(s) 449, 566
Eco57I CTGAAG 1 cut(s) 878
EcoNI CCTNNNNNAGG 1 cut(s) 857
EcoRII CCWGG 3 cut(s) 261, 348, 445
EcoRV GATATC 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 6 cut(s) 155, 367, 394, 445, 817, 823
FatI CATG 6 cut(s) 151, 363, 390, 441, 813, 819
FbaI TGATCA 3 cut(s) 306, 526, 762
Fnu4HI GCNGC 3 cut(s) 240, 417, 420
FokI GGATG 2 cut(s) 229, 772
Fsp4HI GCNGC 3 cut(s) 240, 417, 420
FspBI CTAG 3 cut(s) 617, 623, 828
GluI GCNGC 3 cut(s) 240, 417, 420
HaeIII GGCC 3 cut(s) 357, 374, 536
HapII CCGG 1 cut(s) 246
Hin1II CATG 6 cut(s) 155, 367, 394, 445, 817, 823
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HinfI GANTC 2 cut(s) 87, 484
HpaII CCGG 1 cut(s) 246
HphI GGTGA 2 cut(s) 34, 805
Hpy166II GTNNAC 2 cut(s) 504, 592
Hpy188I TCNGA 3 cut(s) 289, 459, 808
Hpy188III TCNNGA 6 cut(s) 488, 585, 617, 766, 828, 856
Hpy8I GTNNAC 2 cut(s) 504, 592
HpyAV CCTTC 2 cut(s) 784, 853
HpyCH4IV ACGT 2 cut(s) 294, 755
HpyCH4V TGCA 4 cut(s) 4, 239, 419, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 142, 428
HpyF3I CTNAG 6 cut(s) 286, 405, 458, 513, 678, 867
HpySE526I ACGT 2 cut(s) 294, 755
Hsp92II CATG 6 cut(s) 155, 367, 394, 445, 817, 823
Ksp22I TGATCA 3 cut(s) 306, 526, 762
Kzo9I GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
LmnI GCTCC 3 cut(s) 155, 167, 838
Lsp1109I GCAGC 3 cut(s) 251, 403, 431
LweI GCATC 1 cut(s) 251
MaeI CTAG 3 cut(s) 617, 623, 828
MaeII ACGT 2 cut(s) 294, 755
MaeIII GTNAC 5 cut(s) 212, 220, 327, 461, 719
MalI GATC 8 cut(s) 260, 308, 496, 528, 615, 747, 764, 810
MboI GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
MboII GAAGA 4 cut(s) 142, 493, 573, 674
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 2 cut(s) 613, 745
MlsI TGGCCA 1 cut(s) 357
MluCI AATT 3 cut(s) 311, 384, 411
MluNI TGGCCA 1 cut(s) 357
MmeI TCCRAC 2 cut(s) 572, 873
Mox20I TGGCCA 1 cut(s) 357
MscI TGGCCA 1 cut(s) 357
MseI TTAA 2 cut(s) 467, 880
MslI CAYNNNNRTG 1 cut(s) 818
Msp20I TGGCCA 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 3 cut(s) 263, 350, 447
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 3 cut(s) 263, 350, 447
MwoI GCNNNNNNNGC 3 cut(s) 55, 142, 428
NcoI CCATGG 1 cut(s) 390
NdeII GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
NlaIII CATG 6 cut(s) 155, 367, 394, 445, 817, 823
NlaIV GGNNCC 5 cut(s) 373, 401, 535, 699, 805
NmuCI GTSAC 2 cut(s) 212, 719
PfeI GAWTC 2 cut(s) 87, 484
PflMI CCANNNNNTGG 1 cut(s) 364
PkrI GCNGC 3 cut(s) 241, 418, 421
Psp6I CCWGG 3 cut(s) 261, 348, 445
PspGI CCWGG 3 cut(s) 261, 348, 445
PspN4I GGNNCC 5 cut(s) 373, 401, 535, 699, 805
PspPI GGNCC 4 cut(s) 372, 449, 534, 566
PsrI GAACNNNNNNTAC 2 cut(s) 258, 290
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 2 cut(s) 613, 745
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 4 cut(s) 94, 267, 593, 689
RsaNI GTAC 4 cut(s) 93, 266, 592, 688
RseI CAYNNNNRTG 1 cut(s) 818
SaqAI TTAA 2 cut(s) 467, 880
SatI GCNGC 3 cut(s) 240, 417, 420
Sau3AI GATC 8 cut(s) 258, 306, 494, 526, 613, 745, 762, 808
Sau96I GGNCC 4 cut(s) 372, 449, 534, 566
ScrFI CCNGG 3 cut(s) 263, 350, 447
SfaNI GCATC 1 cut(s) 251
SfcI CTRYAG 3 cut(s) 207, 228, 417
SinI GGWCC 2 cut(s) 449, 566
SmiMI CAYNNNNRTG 1 cut(s) 818
Sse9I AATT 3 cut(s) 311, 384, 411
SsiI CCGC 1 cut(s) 165
SspI AATATT 3 cut(s) 100, 575, 875
SspMI CTAG 3 cut(s) 617, 623, 828
StyD4I CCNGG 3 cut(s) 261, 348, 445
StyI CCWWGG 1 cut(s) 390
TaiI ACGT 2 cut(s) 297, 758
TaqI TCGA 1 cut(s) 584
TasI AATT 3 cut(s) 311, 384, 411
TatI WGTACW 1 cut(s) 92
TfiI GAWTC 2 cut(s) 87, 484
Tru1I TTAA 2 cut(s) 467, 880
Tru9I TTAA 2 cut(s) 467, 880
TscAI CASTG 2 cut(s) 439, 682
TseFI GTSAC 2 cut(s) 212, 719
TseI GCWGC 3 cut(s) 239, 416, 419
Tsp45I GTSAC 2 cut(s) 212, 719
TspDTI ATGAA 5 cut(s) 140, 266, 430, 700, 798
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 2 cut(s) 439, 682
Van91I CCANNNNNTGG 1 cut(s) 364
VpaK11BI GGWCC 2 cut(s) 449, 566
XagI CCTNNNNNAGG 1 cut(s) 857
XapI RAATTY 1 cut(s) 311
XbaI TCTAGA 2 cut(s) 616, 827
XcmI CCANNNNNNNNNTGG 2 cut(s) 122, 365
XspI CTAG 3 cut(s) 617, 623, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.