Rw2G013050

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000171
Physical Location & Seq
Forward (+)
13592346 .. 13593480
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013050.1

Sequence Viewer

Length: 846 bp
ATGAGAGTGAAGCATTTTGTGCCTTTTCTTTTGCTTCTTTGCTTGGCAAGAATCTGTACTAGTATTGTTGCAGCTGACCAAATCTCATCTGGGAAGAAGCATTTTGTGCTGGTTCATGGAGCTGGTCACGGAGCGTGGTGTTGGTATAAGCTGGCAACTCTATTGAGCTCTGCAGGTCACAATGTTACAACTCTAGACCTTGCAGCATCTGGTATCAACCCGATCCAGGTACAGCAAGTTCATTCGTTCTCAGACTATGTTGAGCCATTGATCAAATTTCTAGGGTCTCTGTTACCAAAGGAGAAGGTTATCCTGGTTGGCCACAGCATGGGTGGGGCGGTGATATCAATTGCCATGGAGAGGTTCCCTGAGAAAATTGCTGCAGCTGTTTATGCCACTGCTTTTATGCCTGGTCCTGCTCTGAGTTACTTGACTATTTTTGAGAAGATTCAGGAAAGATCGGTGTTGACTGACACTCAGTATAGATATGATCAGGGGGCCAACAATCCTCCTACCTCGCTACTCTTTGGACCAAAACAATTGTCTTCGAGACTGTACCAGCTATCACCACCAGAGGATCTAGAACTAGCATTGTCATTGGTGAGATTTTCCCCTCTATTTATTGAAGAAATAAAACTCACTGAGGAGAAGTACGGATTGGTTCCAAGAGTTTTCATCGTGTGTGACCAAGACCTTTCAATAGAGGAGGATATGCAAACGTGGATGATCAGGAAGAATCCCCCAAATGAAGTGAAGGTGATAAATGGTTCCGATCACATGGTCATGTTCTCTAGATCATTGGAGCTGTTCTCCAACCTCCTGAACGTTGCTGAGAAATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

281

Amino Acids

31.34

Weight (kDa)

6.45

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 32 - 139 1.3e-13 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 34 - 264 1.8e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 35 - 266 7.1e-23 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 328
AciI CCGC 1 cut(s) 338
AclI AACGTT 1 cut(s) 825
AclWI GGATC 2 cut(s) 217, 585
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 275
AfaI GTAC 4 cut(s) 58, 231, 557, 653
AfiI CCNNNNNNNGG 3 cut(s) 226, 328, 360
AgsI TTSAA 2 cut(s) 626, 699
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 3 cut(s) 225, 312, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 7 cut(s) 74, 122, 151, 168, 386, 562, 805
AluI AGCT 7 cut(s) 74, 122, 151, 168, 386, 562, 805
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 291, 544
AlwI GGATC 2 cut(s) 217, 585
AlwNI CAGNNNCTG 1 cut(s) 209
AoxI GGCC 2 cut(s) 319, 498
ApeKI GCWGC 4 cut(s) 71, 203, 380, 383
ApoI RAATTY 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AspS9I GGNCC 3 cut(s) 413, 498, 530
AsuHPI GGTGA 4 cut(s) 352, 558, 613, 769
AvaII GGWCC 2 cut(s) 413, 530
BalI TGGCCA 1 cut(s) 321
BanII GRGCYC 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 4 cut(s) 83, 215, 367, 395
BciT130I CCWGG 3 cut(s) 227, 314, 411
BclI TGATCA 3 cut(s) 270, 490, 726
BcoDI GTCTC 2 cut(s) 291, 544
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 6 cut(s) 60, 194, 281, 581, 587, 792
BfmI CTRYAG 2 cut(s) 171, 381
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 4 cut(s) 72, 204, 381, 384
BlsI GCNGC 4 cut(s) 73, 205, 382, 385
Bme1390I CCNGG 3 cut(s) 227, 314, 411
Bme18I GGWCC 2 cut(s) 413, 530
BmgT120I GGNCC 3 cut(s) 413, 498, 530
BmiI GGNNCC 4 cut(s) 365, 499, 663, 769
BmrFI CCNGG 3 cut(s) 227, 314, 411
BmsI GCATC 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 537
BplI GAGNNNNNCTC 2 cut(s) 794, 826
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 328, 360
BseBI CCWGG 3 cut(s) 227, 314, 411
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 729
BseLI CCNNNNNNNGG 3 cut(s) 226, 328, 360
BseMII CTCAG 6 cut(s) 264, 360, 413, 491, 633, 822
BseRI GAGGAG 2 cut(s) 659, 719
BseXI GCAGC 4 cut(s) 83, 215, 367, 395
BshFI GGCC 2 cut(s) 321, 500
BsiHKAI GWGCWC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 226, 328, 360
BsmAI GTCTC 2 cut(s) 291, 544
BsnI GGCC 2 cut(s) 321, 500
Bso31I GGTCTC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 1 cut(s) 338
BspANI GGCC 2 cut(s) 321, 500
BspCNI CTCAG 6 cut(s) 263, 361, 414, 490, 634, 823
BspLI GGNNCC 4 cut(s) 365, 499, 663, 769
BspMAI CTGCAG 2 cut(s) 175, 385
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 2 cut(s) 217, 585
BspTNI GGTCTC 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 3 cut(s) 227, 314, 411
Bst4CI ACNGT 1 cut(s) 555
BstAPI GCANNNNNTGC 2 cut(s) 19, 106
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 6 cut(s) 250, 369, 422, 477, 642, 831
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 729
BstKTI GATC 8 cut(s) 225, 273, 461, 493, 580, 729, 775, 797
BstMAI GTCTC 2 cut(s) 291, 544
BstMBI GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
BstMWI GCNNNNNNNGC 3 cut(s) 19, 106, 392
BstNI CCWGG 3 cut(s) 227, 314, 411
BstSCI CCNGG 3 cut(s) 225, 312, 409
BstSFI CTRYAG 2 cut(s) 171, 381
BstV1I GCAGC 4 cut(s) 83, 215, 367, 395
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuRI GGCC 2 cut(s) 321, 500
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 729
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 396, 639
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 1 cut(s) 209
Cfr13I GGNCC 3 cut(s) 413, 498, 530
Csp6I GTAC 4 cut(s) 57, 230, 556, 652
CviAII CATG 5 cut(s) 116, 328, 355, 778, 784
CviQI GTAC 4 cut(s) 57, 230, 556, 652
DdeI CTNAG 6 cut(s) 250, 369, 422, 477, 642, 831
DpnI GATC 8 cut(s) 224, 272, 460, 492, 579, 728, 774, 796
DpnII GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
EaeI YGGCCR 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 291
Eco32I GATATC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 413, 530
Eco53kI GAGCTC 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 168
EcoRII CCWGG 3 cut(s) 225, 312, 409
EcoRV GATATC 1 cut(s) 345
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 5 cut(s) 119, 331, 358, 781, 787
FatI CATG 5 cut(s) 115, 327, 354, 777, 783
FbaI TGATCA 3 cut(s) 270, 490, 726
Fnu4HI GCNGC 4 cut(s) 72, 204, 381, 384
FokI GGATG 1 cut(s) 736
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 72, 204, 381, 384
FspBI CTAG 6 cut(s) 60, 194, 281, 581, 587, 792
GluI GCNGC 4 cut(s) 72, 204, 381, 384
HaeIII GGCC 2 cut(s) 321, 500
Hin1II CATG 5 cut(s) 119, 331, 358, 781, 787
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 3 cut(s) 51, 448, 736
HphI GGTGA 4 cut(s) 352, 558, 613, 769
Hpy166II GTNNAC 1 cut(s) 468
Hpy188I TCNGA 3 cut(s) 253, 423, 772
Hpy188III TCNNGA 7 cut(s) 194, 452, 549, 581, 730, 792, 820
Hpy8I GTNNAC 1 cut(s) 468
HpyAV CCTTC 2 cut(s) 298, 748
HpyCH4III ACNGT 1 cut(s) 555
HpyCH4IV ACGT 2 cut(s) 719, 825
HpyCH4V TGCA 5 cut(s) 71, 173, 203, 383, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 106, 392
HpyF3I CTNAG 6 cut(s) 250, 369, 422, 477, 642, 831
HpySE526I ACGT 2 cut(s) 719, 825
Hsp92II CATG 5 cut(s) 119, 331, 358, 781, 787
Ksp22I TGATCA 3 cut(s) 270, 490, 726
Kzo9I GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
LmnI GCTCC 3 cut(s) 119, 131, 802
Lsp1109I GCAGC 4 cut(s) 83, 215, 367, 395
LweI GCATC 1 cut(s) 215
MaeI CTAG 6 cut(s) 60, 194, 281, 581, 587, 792
MaeII ACGT 2 cut(s) 719, 825
MaeIII GTNAC 6 cut(s) 125, 176, 184, 291, 425, 683
MalI GATC 8 cut(s) 224, 272, 460, 492, 579, 728, 774, 796
MboI GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
MboII GAAGA 5 cut(s) 106, 457, 537, 638, 745
MfeI CAATTG 2 cut(s) 348, 539
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 170
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 4 cut(s) 275, 348, 375, 539
MluNI TGGCCA 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 837
MnlI CCTC 9 cut(s) 354, 519, 526, 568, 624, 637, 697, 700, 827
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MseI TTAA 1 cut(s) 844
MslI CAYNNNNRTG 1 cut(s) 782
Msp20I TGGCCA 1 cut(s) 321
MspA1I CMGCKG 2 cut(s) 74, 386
MspR9I CCNGG 3 cut(s) 227, 314, 411
MunI CAATTG 2 cut(s) 348, 539
MvaI CCWGG 3 cut(s) 227, 314, 411
MwoI GCNNNNNNNGC 3 cut(s) 19, 106, 392
NcoI CCATGG 1 cut(s) 354
NdeII GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
NlaIII CATG 5 cut(s) 119, 331, 358, 781, 787
NlaIV GGNNCC 4 cut(s) 365, 499, 663, 769
NmuCI GTSAC 3 cut(s) 125, 176, 683
PfeI GAWTC 3 cut(s) 51, 448, 736
PflMI CCANNNNNTGG 1 cut(s) 328
PkrI GCNGC 4 cut(s) 73, 205, 382, 385
Psp124BI GAGCTC 1 cut(s) 170
Psp1406I AACGTT 1 cut(s) 825
Psp6I CCWGG 3 cut(s) 225, 312, 409
PspGI CCWGG 3 cut(s) 225, 312, 409
PspN4I GGNNCC 4 cut(s) 365, 499, 663, 769
PspPI GGNCC 3 cut(s) 413, 498, 530
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstI CTGCAG 2 cut(s) 175, 385
PstNI CAGNNNCTG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 2 cut(s) 74, 386
RsaI GTAC 4 cut(s) 58, 231, 557, 653
RsaNI GTAC 4 cut(s) 57, 230, 556, 652
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 170
SaqAI TTAA 1 cut(s) 844
SatI GCNGC 4 cut(s) 72, 204, 381, 384
Sau3AI GATC 8 cut(s) 222, 270, 458, 490, 577, 726, 772, 794
Sau96I GGNCC 3 cut(s) 413, 498, 530
ScrFI CCNGG 3 cut(s) 227, 314, 411
SduI GDGCHC 1 cut(s) 170
SfaNI GCATC 1 cut(s) 215
SfcI CTRYAG 2 cut(s) 171, 381
SinI GGWCC 2 cut(s) 413, 530
SmiMI CAYNNNNRTG 1 cut(s) 782
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 4 cut(s) 275, 348, 375, 539
SsiI CCGC 1 cut(s) 338
SspI AATATT 1 cut(s) 839
SspMI CTAG 6 cut(s) 60, 194, 281, 581, 587, 792
SstI GAGCTC 1 cut(s) 170
StyD4I CCNGG 3 cut(s) 225, 312, 409
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 1 cut(s) 555
TaiI ACGT 2 cut(s) 722, 828
TaqI TCGA 1 cut(s) 548
TasI AATT 4 cut(s) 275, 348, 375, 539
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 3 cut(s) 51, 448, 736
Tru1I TTAA 1 cut(s) 844
Tru9I TTAA 1 cut(s) 844
TscAI CASTG 2 cut(s) 403, 646
TseFI GTSAC 3 cut(s) 125, 176, 683
TseI GCWGC 4 cut(s) 71, 203, 380, 383
Tsp45I GTSAC 3 cut(s) 125, 176, 683
TspDTI ATGAA 4 cut(s) 104, 230, 664, 762
TspGWI ACGGA 2 cut(s) 144, 669
TspRI CASTG 2 cut(s) 403, 646
Van91I CCANNNNNTGG 1 cut(s) 328
VpaK11BI GGWCC 2 cut(s) 413, 530
XapI RAATTY 1 cut(s) 275
XbaI TCTAGA 3 cut(s) 193, 580, 791
XcmI CCANNNNNNNNNTGG 2 cut(s) 86, 329
XspI CTAG 6 cut(s) 60, 194, 281, 581, 587, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.