MD02G1162200.v1.1

Salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000171
Physical Location & Seq
Forward (+)
13685248 .. 13691212
5965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1162200.v1.1.491

Sequence Viewer

Length: 888 bp
ATGATACAGAAATTGGAATTGAAGAACATGGTAGAGAAGCTCCTCTTGTTTTTCCTTTTATTTATTTGCTTGGCAAAAGCGAGTACCTCAACCCCATCCCTGCCCTCCAACATTCACAACAAAACTAAAAGTCCAAAACATTTTGTGTTGATACATGGAGCCTGTCATGGGGCATGGAGTTGGTATAAGGTGGCGACTCTTCTGAGGAACTCAGGTCACAATGTAACAGCTCTAGACTTGGGAGCATCCGGGATCAACCCGATTCAGGTACAACAACTCCCTTCGTTATCGGAATTCGTCGAGCCTTTGACAAAGGTCATGGTGTCTCTACTACCAAATGAAAAGGTTATCCTCGTGGGTCACAGCTTGGGTGGAGCTGTCATATCTATTTTCATGGAGAGGTTTCCTCATAAAGTTGCTGCTGCGGTATTTGTCACAGCTATCATGTATGGTCCTACTCTCAATTTCTCAACTGTACATGCAGAGGTTACCAAAGGAAGGGATTTAATGGACACTCAATACAGATATGACAACGGGACCAACAACCCTGCAACCTCCCAAGTCTTTGGACCTAAGTTTATGGCGACAAGCTTGTACCAGCTCTCCCCACCACAGGATTTAAATCTAGCGTTATCGTTGGTGAGACTTGTTCCTTTATATAATTATGATGTAATAAAACTCACGAAGGAGAAGTATGGATCGGTTCGTAGAGTATTCATCGTGGCCGACCAAGACCATGCGATTGCGTTGGATGCGCAAAATTATATGATCAAGAACAATCCCCCAAATGAAGTGAAAGTGATAAACGGTTCTGATCACATGGTCATGTTCTCTAAACCAGTGGAGCTGTTCTGCCACCTCCAAAGTATTGCTGAGAACCATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

296

Amino Acids

32.77

Weight (kDa)

9.0

Isoelectric Point (pI)

32.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 47 - 280 2.1e-21 alpha/beta hydrolase fold
Hydrolase_4 PF12146 47 - 152 2.7e-10 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 48 - 284 7.1e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 756
AciI CCGC 1 cut(s) 425
AclWI GGATC 2 cut(s) 260, 706
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 1 cut(s) 293
AfaI GTAC 4 cut(s) 85, 270, 477, 596
AfiI CCNNNNNNNGG 4 cut(s) 168, 265, 498, 613
AgsI TTSAA 1 cut(s) 22
AluBI AGCT 8 cut(s) 40, 230, 366, 377, 440, 591, 601, 847
AluI AGCT 8 cut(s) 40, 230, 366, 377, 440, 591, 601, 847
Alw26I GTCTC 2 cut(s) 330, 637
AlwI GGATC 2 cut(s) 260, 706
AoxI GGCC 1 cut(s) 723
ApeKI GCWGC 2 cut(s) 419, 422
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 3 cut(s) 452, 537, 569
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 652
AvaII GGWCC 3 cut(s) 452, 537, 569
BauI CACGAG 1 cut(s) 353
BbvI GCAGC 2 cut(s) 406, 409
BccI CCATC 1 cut(s) 103
BclI TGATCA 2 cut(s) 768, 814
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 330, 637
BfaI CTAG 2 cut(s) 233, 626
BisI GCNGC 2 cut(s) 420, 423
BlsI GCNGC 2 cut(s) 421, 424
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 3 cut(s) 452, 537, 569
BmgT120I GGNCC 3 cut(s) 452, 537, 569
BmiI GGNNCC 2 cut(s) 160, 538
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 254, 742
BoxI GACNNNNGTC 1 cut(s) 314
BplI GAGNNNNNCTC 2 cut(s) 391, 423
BpuMI CCSGG 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 621
BsaXI ACNNNNNCTCC 4 cut(s) 261, 291, 587, 617
Bsc4I CCNNNNNNNGG 4 cut(s) 168, 265, 498, 613
Bse1I ACTGG 1 cut(s) 839
Bse8I GATNNNNATC 1 cut(s) 621
BseGI GGATG 3 cut(s) 95, 245, 757
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 4 cut(s) 168, 265, 498, 613
BseMII CTCAG 3 cut(s) 194, 225, 864
BseNI ACTGG 1 cut(s) 839
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 406, 409
BshFI GGCC 1 cut(s) 725
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 4 cut(s) 168, 265, 498, 613
BsmAI GTCTC 2 cut(s) 330, 637
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 1 cut(s) 725
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 4 cut(s) 252, 698, 768, 814
BspACI CCGC 1 cut(s) 425
BspANI GGCC 1 cut(s) 725
BspCNI CTCAG 3 cut(s) 195, 224, 865
BspLI GGNNCC 2 cut(s) 160, 538
BspPI GGATC 2 cut(s) 260, 706
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 1 cut(s) 839
BssMI GATC 4 cut(s) 252, 698, 768, 814
BssSI CACGAG 1 cut(s) 353
Bst2BI CACGAG 1 cut(s) 353
Bst4CI ACNGT 2 cut(s) 475, 809
Bst6I CTCTTC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 475
BstDEI CTNAG 4 cut(s) 203, 211, 573, 873
BstEII GGTNACC 1 cut(s) 487
BstF5I GGATG 3 cut(s) 95, 245, 757
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 4 cut(s) 255, 701, 771, 817
BstMAI GTCTC 2 cut(s) 330, 637
BstMBI GATC 4 cut(s) 252, 698, 768, 814
BstMWI GCNNNNNNNGC 1 cut(s) 752
BstNSI RCATGY 1 cut(s) 482
BstPAI GACNNNNGTC 1 cut(s) 314
BstPI GGTNACC 1 cut(s) 487
BstSCI CCNGG 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 406, 409
BstXI CCANNNNNNTGG 1 cut(s) 566
BsuRI GGCC 1 cut(s) 725
BtsCI GGATG 3 cut(s) 95, 245, 757
BtsIMutI CAGTG 1 cut(s) 846
CfoI GCGC 1 cut(s) 757
Cfr13I GGNCC 3 cut(s) 452, 537, 569
Csp6I GTAC 4 cut(s) 84, 269, 476, 595
CviQI GTAC 4 cut(s) 84, 269, 476, 595
DdeI CTNAG 4 cut(s) 203, 211, 573, 873
DpnI GATC 4 cut(s) 254, 700, 770, 816
DpnII GATC 4 cut(s) 252, 698, 768, 814
DraI TTTAAA 1 cut(s) 621
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco47I GGWCC 3 cut(s) 452, 537, 569
Eco91I GGTNACC 1 cut(s) 487
EcoO65I GGTNACC 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 293
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 550
FbaI TGATCA 2 cut(s) 768, 814
Fnu4HI GCNGC 2 cut(s) 420, 423
FokI GGATG 3 cut(s) 82, 232, 764
Fsp4HI GCNGC 2 cut(s) 420, 423
FspBI CTAG 2 cut(s) 233, 626
FspI TGCGCA 1 cut(s) 756
GlaI GCGC 1 cut(s) 756
GluI GCNGC 2 cut(s) 420, 423
HaeIII GGCC 1 cut(s) 725
HapII CCGG 1 cut(s) 249
HhaI GCGC 1 cut(s) 757
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HindIII AAGCTT 1 cut(s) 589
HinfI GANTC 2 cut(s) 196, 262
HpaII CCGG 1 cut(s) 249
HphI GGTGA 1 cut(s) 652
Hpy188I TCNGA 3 cut(s) 204, 292, 814
Hpy188III TCNNGA 3 cut(s) 233, 682, 772
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 291, 492, 679
HpyCH4III ACNGT 2 cut(s) 475, 809
HpyCH4V TGCA 2 cut(s) 482, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 752
HpyF3I CTNAG 4 cut(s) 203, 211, 573, 873
HspAI GCGC 1 cut(s) 755
Ksp22I TGATCA 2 cut(s) 768, 814
Kzo9I GATC 4 cut(s) 252, 698, 768, 814
LmnI GCTCC 5 cut(s) 45, 158, 242, 374, 844
LpnPI CCDG 9 cut(s) 113, 175, 198, 251, 262, 561, 599, 611, 852
Lsp1109I GCAGC 2 cut(s) 406, 409
LweI GCATC 2 cut(s) 254, 742
MaeI CTAG 2 cut(s) 233, 626
MaeIII GTNAC 5 cut(s) 215, 223, 359, 433, 487
MalI GATC 4 cut(s) 254, 700, 770, 816
MboI GATC 4 cut(s) 252, 698, 768, 814
MboII GAAGA 2 cut(s) 34, 191
MluCI AATT 6 cut(s) 11, 17, 293, 463, 661, 760
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 2 cut(s) 132, 729
MseI TTAA 2 cut(s) 506, 620
MslI CAYNNNNRTG 1 cut(s) 824
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 752
NciI CCSGG 1 cut(s) 250
NdeII GATC 4 cut(s) 252, 698, 768, 814
NlaIV GGNNCC 2 cut(s) 160, 538
NmuCI GTSAC 3 cut(s) 215, 359, 433
NsbI TGCGCA 1 cut(s) 756
NspI RCATGY 1 cut(s) 482
PfeI GAWTC 1 cut(s) 262
PfoI TCCNGGA 1 cut(s) 248
PkrI GCNGC 2 cut(s) 421, 424
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PshAI GACNNNNGTC 1 cut(s) 314
PspEI GGTNACC 1 cut(s) 487
PspN4I GGNNCC 2 cut(s) 160, 538
PspPI GGNCC 3 cut(s) 452, 537, 569
RsaI GTAC 4 cut(s) 85, 270, 477, 596
RsaNI GTAC 4 cut(s) 84, 269, 476, 595
RseI CAYNNNNRTG 1 cut(s) 824
SaqAI TTAA 2 cut(s) 506, 620
SatI GCNGC 2 cut(s) 420, 423
Sau3AI GATC 4 cut(s) 252, 698, 768, 814
Sau96I GGNCC 3 cut(s) 452, 537, 569
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 250
SfaNI GCATC 2 cut(s) 254, 742
SinI GGWCC 3 cut(s) 452, 537, 569
SmiI ATTTAAAT 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 824
Sse9I AATT 6 cut(s) 11, 17, 293, 463, 661, 760
SsiI CCGC 1 cut(s) 425
SspMI CTAG 2 cut(s) 233, 626
StyD4I CCNGG 1 cut(s) 248
SwaI ATTTAAAT 1 cut(s) 621
TaaI ACNGT 2 cut(s) 475, 809
TaqI TCGA 1 cut(s) 300
TasI AATT 6 cut(s) 11, 17, 293, 463, 661, 760
TatI WGTACW 1 cut(s) 475
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 2 cut(s) 506, 620
Tru9I TTAA 2 cut(s) 506, 620
TscAI CASTG 1 cut(s) 846
TseFI GTSAC 3 cut(s) 215, 359, 433
TseI GCWGC 2 cut(s) 419, 422
Tsp45I GTSAC 3 cut(s) 215, 359, 433
TspDTI ATGAA 5 cut(s) 354, 382, 706, 804, 873
TspRI CASTG 1 cut(s) 846
VpaK11BI GGWCC 3 cut(s) 452, 537, 569
XapI RAATTY 1 cut(s) 293
XbaI TCTAGA 1 cut(s) 232
XceI RCATGY 1 cut(s) 482
XspI CTAG 2 cut(s) 233, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.