Rroxscaffold_2G00139330

Methylketone synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000171
Physical Location & Seq
Reverse (-)
77778474 .. 77779613
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139330.1

Sequence Viewer

Length: 894 bp
ATGGAGAATATAAGAGTGAAGCATTTCGTGCCTTTTCTTTTGCTTCTTTGCTTGGCAAGAATCTGTACTAATATTGTTGAAGCTGACCAAATCTCATCTGGGAAGAAGCATTTTGTGCTGGTTCATGGAGCTGGTCACGGAGCTTGGTGTTGGTATAAGCTGGCAACTCTATTGAACTCTACAGCATCCGGTATCAACCCAATCCAGGTACAGCAAGTTCATTCGTTCTCAGACTACGTTGAGCCATTGATCAAATTTCTGGGGTCTCTGTTACCAAAAGAGAGGGTTATCCTGGTTGGCCACAGCATGGGTGGGGCCGTGATATCAATTGCCATGGAGAGGTTCCCTGAGAAAATTGCTGCAGCTGTTTATGCCACTGCTTTGATGCCTGGTCCTGCTCTGAGTTACTTGACTATTTTTGAGAAGATTCAGGAAAGATCGGTGTTGACTGACACTCAGTATGGATATGATCAGGGGGCCAACAATCCTCCAACCTCGGTACTCTTTGGACCAAAACAATTGTCTTCGAGACTGTACCAGCTATCGCCACCAGAGGTTCAATTTTCAGTTGGTGATCTAATACCGTCCCTGTTTTCTTCTTTGTTATTGATGCATCAATGGACAGGATCTAGAACTGGCATTGTCATTGGTGAGATTTTACCCCTATTTATTGAAGAAATAAAACTCACTGAGGAGAAGTACGGATTGGTTCCAAGAGTTTTCATCGTGTGTGACCAGGACCTTTCATTAGAGGAGGATCTGCAAACGTGGATGATCAGGAAAAATCCCCCAAATGAAGTGAAGGTGATAAATGGTTCCGATCACATGGTCATGTTCTCTAGGCCATTGGAGCTGTTCTCCAACCTCCTTAACGTTGCTGAGAAATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

297

Amino Acids

33.15

Weight (kDa)

6.07

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 37 - 281 1e-10 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 38 - 286 6.8e-18 Alpha/beta hydrolase family
Hydrolase_4 PF12146 72 - 129 7.5e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 307
AclI AACGTT 1 cut(s) 873
AclWI GGATC 2 cut(s) 634, 765
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 1 cut(s) 254
AfaI GTAC 5 cut(s) 67, 210, 501, 536, 701
AfiI CCNNNNNNNGG 3 cut(s) 205, 307, 339
AgsI TTSAA 4 cut(s) 80, 175, 560, 674
AjnI CCWGG 4 cut(s) 204, 291, 388, 735
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AluBI AGCT 7 cut(s) 83, 131, 143, 160, 365, 541, 853
AluI AGCT 7 cut(s) 83, 131, 143, 160, 365, 541, 853
Alw26I GTCTC 2 cut(s) 270, 523
AlwI GGATC 2 cut(s) 634, 765
AoxI GGCC 4 cut(s) 298, 315, 477, 842
ApeKI GCWGC 2 cut(s) 359, 362
ApoI RAATTY 1 cut(s) 254
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
Asp700I GAANNNNTTC 1 cut(s) 23
AspS9I GGNCC 5 cut(s) 315, 392, 477, 509, 739
AsuHPI GGTGA 3 cut(s) 584, 662, 817
AvaII GGWCC 3 cut(s) 392, 509, 739
BalI TGGCCA 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 346, 374
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 4 cut(s) 206, 293, 390, 737
BclI TGATCA 3 cut(s) 249, 469, 774
BcoDI GTCTC 2 cut(s) 270, 523
BfaI CTAG 2 cut(s) 630, 840
BfmI CTRYAG 2 cut(s) 180, 360
BisI GCNGC 2 cut(s) 360, 363
BlsI GCNGC 2 cut(s) 361, 364
Bme1390I CCNGG 4 cut(s) 206, 293, 390, 737
Bme18I GGWCC 3 cut(s) 392, 509, 739
BmgT120I GGNCC 5 cut(s) 315, 392, 477, 509, 739
BmiI GGNNCC 5 cut(s) 316, 344, 478, 711, 817
BmrFI CCNGG 4 cut(s) 206, 293, 390, 737
BmsI GCATC 4 cut(s) 194, 375, 600, 622
BpiI GAAGAC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 842, 874
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 333, 495
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 307, 339
Bse1I ACTGG 1 cut(s) 640
BseBI CCWGG 4 cut(s) 206, 293, 390, 737
BseDI CCNNGG 2 cut(s) 333, 495
BseGI GGATG 2 cut(s) 185, 777
BseLI CCNNNNNNNGG 3 cut(s) 205, 307, 339
BseMII CTCAG 6 cut(s) 243, 339, 392, 470, 681, 870
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 2 cut(s) 707, 767
BseXI GCAGC 2 cut(s) 346, 374
BshFI GGCC 4 cut(s) 300, 317, 479, 844
BsiSI CCGG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 3 cut(s) 205, 307, 339
BsmAI GTCTC 2 cut(s) 270, 523
BsmFI GGGAC 1 cut(s) 571
BsnI GGCC 4 cut(s) 300, 317, 479, 844
Bso31I GGTCTC 1 cut(s) 270
Bsp143I GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
Bsp19I CCATGG 1 cut(s) 333
BspANI GGCC 4 cut(s) 300, 317, 479, 844
BspCNI CTCAG 6 cut(s) 242, 340, 393, 469, 682, 871
BspLI GGNNCC 5 cut(s) 316, 344, 478, 711, 817
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 2 cut(s) 634, 765
BspTNI GGTCTC 1 cut(s) 270
BsrI ACTGG 1 cut(s) 640
BssECI CCNNGG 2 cut(s) 333, 495
BssMI GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 4 cut(s) 206, 293, 390, 737
Bst4CI ACNGT 2 cut(s) 534, 585
BstAPI GCANNNNNTGC 2 cut(s) 28, 115
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 6 cut(s) 229, 348, 401, 456, 690, 879
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 185, 777
BstKTI GATC 8 cut(s) 252, 440, 472, 577, 629, 760, 777, 823
BstMAI GTCTC 2 cut(s) 270, 523
BstMBI GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
BstMWI GCNNNNNNNGC 4 cut(s) 28, 115, 371, 850
BstNI CCWGG 4 cut(s) 206, 293, 390, 737
BstSCI CCNGG 4 cut(s) 204, 291, 388, 735
BstSFI CTRYAG 2 cut(s) 180, 360
BstV1I GCAGC 2 cut(s) 346, 374
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 2 cut(s) 626, 757
BstYI RGATCY 2 cut(s) 626, 757
BsuRI GGCC 4 cut(s) 300, 317, 479, 844
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 2 cut(s) 185, 777
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 2 cut(s) 375, 687
Cac8I GCNNGC 1 cut(s) 162
Cfr13I GGNCC 5 cut(s) 315, 392, 477, 509, 739
Csp6I GTAC 5 cut(s) 66, 209, 500, 535, 700
CviAII CATG 5 cut(s) 125, 307, 334, 826, 832
CviQI GTAC 5 cut(s) 66, 209, 500, 535, 700
DdeI CTNAG 6 cut(s) 229, 348, 401, 456, 690, 879
DpnI GATC 8 cut(s) 251, 439, 471, 576, 628, 759, 776, 822
DpnII GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
EaeI YGGCCR 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 324
Eco47I GGWCC 3 cut(s) 392, 509, 739
EcoO109I RGGNCCY 1 cut(s) 739
EcoRII CCWGG 4 cut(s) 204, 291, 388, 735
EcoRV GATATC 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 5 cut(s) 128, 310, 337, 829, 835
FaqI GGGAC 1 cut(s) 571
FatI CATG 5 cut(s) 124, 306, 333, 825, 831
FbaI TGATCA 3 cut(s) 249, 469, 774
Fnu4HI GCNGC 2 cut(s) 360, 363
FokI GGATG 2 cut(s) 172, 784
Fsp4HI GCNGC 2 cut(s) 360, 363
FspBI CTAG 2 cut(s) 630, 840
GluI GCNGC 2 cut(s) 360, 363
HaeIII GGCC 4 cut(s) 300, 317, 479, 844
HapII CCGG 1 cut(s) 189
Hin1II CATG 5 cut(s) 128, 310, 337, 829, 835
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 2 cut(s) 60, 427
HpaII CCGG 1 cut(s) 189
HphI GGTGA 3 cut(s) 584, 662, 817
Hpy166II GTNNAC 1 cut(s) 447
Hpy188I TCNGA 3 cut(s) 232, 402, 820
Hpy188III TCNNGA 4 cut(s) 431, 528, 630, 778
Hpy8I GTNNAC 1 cut(s) 447
HpyAV CCTTC 1 cut(s) 796
HpyCH4III ACNGT 2 cut(s) 534, 585
HpyCH4IV ACGT 3 cut(s) 237, 767, 873
HpyCH4V TGCA 3 cut(s) 362, 613, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 115, 371, 850
HpyF3I CTNAG 6 cut(s) 229, 348, 401, 456, 690, 879
HpySE526I ACGT 3 cut(s) 237, 767, 873
Hsp92II CATG 5 cut(s) 128, 310, 337, 829, 835
Ksp22I TGATCA 3 cut(s) 249, 469, 774
Kzo9I GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
LmnI GCTCC 3 cut(s) 128, 140, 850
Lsp1109I GCAGC 2 cut(s) 346, 374
LweI GCATC 4 cut(s) 194, 375, 600, 622
MaeI CTAG 2 cut(s) 630, 840
MaeII ACGT 3 cut(s) 237, 767, 873
MaeIII GTNAC 4 cut(s) 134, 270, 404, 731
MalI GATC 8 cut(s) 251, 439, 471, 576, 628, 759, 776, 822
MboI GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
MboII GAAGA 5 cut(s) 115, 436, 516, 588, 686
MfeI CAATTG 2 cut(s) 327, 518
MflI RGATCY 2 cut(s) 626, 757
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 5 cut(s) 254, 327, 354, 518, 560
MluNI TGGCCA 1 cut(s) 300
MmeI TCCRAC 2 cut(s) 515, 885
MnlI CCTC 9 cut(s) 276, 333, 498, 505, 547, 685, 745, 748, 875
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 615
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 300
MseI TTAA 2 cut(s) 870, 892
MslI CAYNNNNRTG 1 cut(s) 830
Msp20I TGGCCA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 189
MspR9I CCNGG 4 cut(s) 206, 293, 390, 737
MunI CAATTG 2 cut(s) 327, 518
MvaI CCWGG 4 cut(s) 206, 293, 390, 737
MwoI GCNNNNNNNGC 4 cut(s) 28, 115, 371, 850
NcoI CCATGG 1 cut(s) 333
NdeII GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
NlaIII CATG 5 cut(s) 128, 310, 337, 829, 835
NlaIV GGNNCC 5 cut(s) 316, 344, 478, 711, 817
NmuCI GTSAC 2 cut(s) 134, 731
NsiI ATGCAT 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 60, 427
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 2 cut(s) 361, 364
PpuMI RGGWCCY 1 cut(s) 739
Psp1406I AACGTT 1 cut(s) 873
Psp5II RGGWCCY 1 cut(s) 739
Psp6I CCWGG 4 cut(s) 204, 291, 388, 735
PspGI CCWGG 4 cut(s) 204, 291, 388, 735
PspN4I GGNNCC 5 cut(s) 316, 344, 478, 711, 817
PspPI GGNCC 5 cut(s) 315, 392, 477, 509, 739
PspPPI RGGWCCY 1 cut(s) 739
PsrI GAACNNNNNNTAC 2 cut(s) 201, 233
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 2 cut(s) 626, 757
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 5 cut(s) 67, 210, 501, 536, 701
RsaNI GTAC 5 cut(s) 66, 209, 500, 535, 700
RseI CAYNNNNRTG 1 cut(s) 830
SaqAI TTAA 2 cut(s) 870, 892
SatI GCNGC 2 cut(s) 360, 363
Sau3AI GATC 8 cut(s) 249, 437, 469, 574, 626, 757, 774, 820
Sau96I GGNCC 5 cut(s) 315, 392, 477, 509, 739
ScrFI CCNGG 4 cut(s) 206, 293, 390, 737
SfaNI GCATC 4 cut(s) 194, 375, 600, 622
SfcI CTRYAG 2 cut(s) 180, 360
SinI GGWCC 3 cut(s) 392, 509, 739
SmiMI CAYNNNNRTG 1 cut(s) 830
Sse9I AATT 5 cut(s) 254, 327, 354, 518, 560
SspI AATATT 2 cut(s) 73, 887
SspMI CTAG 2 cut(s) 630, 840
StyD4I CCNGG 4 cut(s) 204, 291, 388, 735
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 534, 585
TaiI ACGT 3 cut(s) 240, 770, 876
TaqI TCGA 1 cut(s) 527
TasI AATT 5 cut(s) 254, 327, 354, 518, 560
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 2 cut(s) 60, 427
Tru1I TTAA 2 cut(s) 870, 892
Tru9I TTAA 2 cut(s) 870, 892
TscAI CASTG 2 cut(s) 382, 694
TseFI GTSAC 2 cut(s) 134, 731
TseI GCWGC 2 cut(s) 359, 362
Tsp45I GTSAC 2 cut(s) 134, 731
TspDTI ATGAA 5 cut(s) 113, 209, 712, 735, 810
TspGWI ACGGA 2 cut(s) 153, 717
TspRI CASTG 2 cut(s) 382, 694
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 392, 509, 739
XapI RAATTY 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 2 cut(s) 95, 308
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 630, 840
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.