Rroxscaffold_2G00139230

salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000171
Physical Location & Seq
Reverse (-)
77596083 .. 77598750
2668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139230.1

Sequence Viewer

Length: 882 bp
ATGGAAATCAAAGAAAAAATGGAGCGGATAAGATCTGTGAAGGAATTAGTTGTGTATTTTCTTATATTTCTTAGCTTGGTAGCAAGTTGCAGTACTAGTAGTGCAGCTTCTTCTTCCCAGATCCCAGTTGGGAAGCACTTTGTGTTAATTCATGGAGCCGGACACGGAGCGTGGTCTTGGTACAAGGTGGCGACTTTACTGAAATACTCGGGTCATAAGGTAACGGCTCTGGACTTGGGAGCATCTGGAATCAACCCGATCCAGGTTCAGCAACTCCCTTCGCTCTCCGACTACGTTGAACCGCTGACGAAGCTCATGGTGTCTCTGCCGCCAAAGGAGAGGGTTATTCTGGTGTCTCACAGCATGACCGGAGCTACAATAGCAGTTTTCATGGAGAGGTTCCCTGAGAAAATTGCGGCAGCCGTGTATGTCTCTGCCTTTATGTCGGGTCCTACTCTCAATTACTCCACCATTTTTACAGAGATAAACAAACGATTGGATTATATGGATTCTGAATATAGATATGATAACGGGACCAACAATTCTGCAACCTCCTTTCGCTTTGGGCCTAGGGTCTTGGCAGCAAGTTTGTACCAGCTATCTCCACAGGATTTAACACTAGGGTTGTCGTTGGTGAGATTTTCTCCTCTATACAACTACGATCTTATAAAACTCACTGAGGAGAAATATGGATCGGTTCCGAGAATATTCATCGTGGCAGATCAAGACCATGCAATAGTTTTAGACGTGCAAAATTTCATGATCAAGAATAATCCACCAAATGAAGTGAAAGTGATCAATGGTGCTGATCACATGGTCATGCTCTCTAGACCCGTGGAGCTCTTCTCCCACCTCCAGAACATTGCTGAGAAATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

293

Amino Acids

32.71

Weight (kDa)

7.81

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 46 - 277 4.9e-15 alpha/beta hydrolase fold
Hydrolase_4 PF12146 46 - 163 2.8e-09 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 47 - 281 7.5e-19 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 668
AccBSI CCGCTC 1 cut(s) 25
AciI CCGC 4 cut(s) 25, 302, 329, 416
AclWI GGATC 3 cut(s) 115, 253, 700
AcsI RAATTY 1 cut(s) 754
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 3 cut(s) 94, 182, 593
AfiI CCNNNNNNNGG 1 cut(s) 262
AgsI TTSAA 1 cut(s) 299
AhlI ACTAGT 1 cut(s) 95
AjiI CACGTC 1 cut(s) 748
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 6 cut(s) 75, 107, 313, 374, 598, 841
AluI AGCT 6 cut(s) 75, 107, 313, 374, 598, 841
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 3 cut(s) 327, 360, 436
AlwI GGATC 3 cut(s) 115, 253, 700
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 1 cut(s) 566
ApeKI GCWGC 3 cut(s) 104, 419, 581
ApoI RAATTY 1 cut(s) 754
AspA2I CCTAGG 1 cut(s) 569
AspS9I GGNCC 3 cut(s) 449, 534, 566
AsuHPI GGTGA 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 2 cut(s) 449, 534
AvrII CCTAGG 1 cut(s) 569
BanII GRGCYC 1 cut(s) 843
BarI GAAGNNNNNNTAC 2 cut(s) 91, 123
Bbv12I GWGCWC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 116, 431, 593
BceAI ACGGC 2 cut(s) 240, 407
BciT130I CCWGG 1 cut(s) 263
BclI TGATCA 3 cut(s) 762, 795, 808
BcoDI GTCTC 3 cut(s) 327, 360, 436
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 4 cut(s) 96, 570, 620, 828
BglII AGATCT 1 cut(s) 32
BisI GCNGC 5 cut(s) 105, 329, 417, 420, 582
BlnI CCTAGG 1 cut(s) 569
BlsI GCNGC 5 cut(s) 106, 330, 418, 421, 583
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 449, 534
BmeT110I CYCGRG 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 748
BmgT120I GGNCC 3 cut(s) 449, 534, 566
BmiI GGNNCC 5 cut(s) 157, 401, 450, 535, 699
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 119
BplI GAGNNNNNCTC 4 cut(s) 628, 660, 830, 862
BpmI CTGGAG 1 cut(s) 839
BsaJI CCNNGG 2 cut(s) 569, 834
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 861
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 569, 834
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 3 cut(s) 396, 669, 858
BseNI ACTGG 1 cut(s) 125
BseRI GAGGAG 2 cut(s) 636, 695
BseXI GCAGC 3 cut(s) 116, 431, 593
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 2 cut(s) 159, 369
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 3 cut(s) 327, 360, 436
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 843
Bsp143I GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
BspACI CCGC 4 cut(s) 25, 302, 329, 416
BspANI GGCC 1 cut(s) 568
BspCNI CTCAG 3 cut(s) 397, 670, 859
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 5 cut(s) 157, 401, 450, 535, 699
BspPI GGATC 3 cut(s) 115, 253, 700
BspQI GCTCTTC 1 cut(s) 848
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 569, 834
BssMI GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
BssT1I CCWWGG 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 263
Bst6I CTCTTC 1 cut(s) 848
BstDEI CTNAG 4 cut(s) 71, 405, 678, 867
BstDSI CCRYGG 1 cut(s) 834
BstKTI GATC 9 cut(s) 35, 123, 261, 664, 695, 724, 765, 798, 811
BstMAI GTCTC 3 cut(s) 327, 360, 436
BstMBI GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
BstMWI GCNNNNNNNGC 2 cut(s) 310, 380
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 116, 431, 593
BstX2I RGATCY 2 cut(s) 32, 120
BstYI RGATCY 2 cut(s) 32, 120
BsuRI GGCC 1 cut(s) 568
BtgI CCRYGG 1 cut(s) 834
BtrI CACGTC 1 cut(s) 748
BtsIMutI CAGTG 1 cut(s) 675
CciI TCATGA 1 cut(s) 759
Cfr13I GGNCC 3 cut(s) 449, 534, 566
Csp6I GTAC 3 cut(s) 93, 181, 592
CviAII CATG 8 cut(s) 152, 316, 364, 391, 731, 760, 814, 820
CviQI GTAC 3 cut(s) 93, 181, 592
DdeI CTNAG 4 cut(s) 71, 405, 678, 867
DpnI GATC 9 cut(s) 34, 122, 260, 663, 694, 723, 764, 797, 810
DpnII GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
DraIII CACNNNGTG 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 848
EarI CTCTTC 1 cut(s) 848
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 843
Eco47I GGWCC 2 cut(s) 449, 534
Eco53kI GAGCTC 1 cut(s) 841
Eco88I CYCGRG 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 841
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 843
ErhI CCWWGG 1 cut(s) 569
FaeI CATG 8 cut(s) 155, 319, 367, 394, 734, 763, 817, 823
FaqI GGGAC 1 cut(s) 547
FatI CATG 8 cut(s) 151, 315, 363, 390, 730, 759, 813, 819
FbaI TGATCA 3 cut(s) 762, 795, 808
Fnu4HI GCNGC 5 cut(s) 105, 329, 417, 420, 582
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 5 cut(s) 105, 329, 417, 420, 582
FspBI CTAG 4 cut(s) 96, 570, 620, 828
GluI GCNGC 5 cut(s) 105, 329, 417, 420, 582
GsuI CTGGAG 1 cut(s) 839
HaeIII GGCC 1 cut(s) 568
HapII CCGG 2 cut(s) 159, 369
Hin1II CATG 8 cut(s) 155, 319, 367, 394, 734, 763, 817, 823
HinfI GANTC 2 cut(s) 249, 509
HpaII CCGG 2 cut(s) 159, 369
HphI GGTGA 1 cut(s) 646
Hpy188I TCNGA 3 cut(s) 289, 514, 702
Hpy188III TCNNGA 7 cut(s) 230, 246, 725, 760, 766, 828, 856
HpyAV CCTTC 2 cut(s) 34, 288
HpyCH4IV ACGT 2 cut(s) 294, 747
HpyCH4V TGCA 5 cut(s) 90, 104, 548, 734, 751
HpyF10VI GCNNNNNNNGC 2 cut(s) 310, 380
HpyF3I CTNAG 4 cut(s) 71, 405, 678, 867
HpySE526I ACGT 2 cut(s) 294, 747
Hsp92II CATG 8 cut(s) 155, 319, 367, 394, 734, 763, 817, 823
Ksp22I TGATCA 3 cut(s) 762, 795, 808
Kzo9I GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
LguI GCTCTTC 1 cut(s) 848
LmnI GCTCC 6 cut(s) 22, 155, 167, 239, 371, 838
Lsp1109I GCAGC 3 cut(s) 116, 431, 593
LweI GCATC 1 cut(s) 251
MaeI CTAG 4 cut(s) 96, 570, 620, 828
MaeII ACGT 2 cut(s) 294, 747
MaeIII GTNAC 1 cut(s) 220
MalI GATC 9 cut(s) 34, 122, 260, 663, 694, 723, 764, 797, 810
MbiI CCGCTC 1 cut(s) 25
MboI GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
MboII GAAGA 3 cut(s) 102, 105, 835
MflI RGATCY 2 cut(s) 32, 120
MhlI GDGCHC 1 cut(s) 843
MluCI AATT 6 cut(s) 44, 147, 411, 460, 541, 754
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 6 cut(s) 333, 390, 562, 657, 673, 863
MseI TTAA 3 cut(s) 146, 614, 880
MslI CAYNNNNRTG 1 cut(s) 818
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 2 cut(s) 159, 369
MspR9I CCNGG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 310, 380
NdeII GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
NlaIII CATG 8 cut(s) 155, 319, 367, 394, 734, 763, 817, 823
NlaIV GGNNCC 5 cut(s) 157, 401, 450, 535, 699
PagI TCATGA 1 cut(s) 759
PciSI GCTCTTC 1 cut(s) 848
PfeI GAWTC 2 cut(s) 249, 509
PkrI GCNGC 5 cut(s) 106, 330, 418, 421, 583
PpuMI RGGWCCY 1 cut(s) 449
PsiI TTATAA 1 cut(s) 668
Psp124BI GAGCTC 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 5 cut(s) 157, 401, 450, 535, 699
PspPI GGNCC 3 cut(s) 449, 534, 566
PspPPI RGGWCCY 1 cut(s) 449
PsuI RGATCY 2 cut(s) 32, 120
RsaI GTAC 3 cut(s) 94, 182, 593
RsaNI GTAC 3 cut(s) 93, 181, 592
RseI CAYNNNNRTG 1 cut(s) 818
SacI GAGCTC 1 cut(s) 843
SapI GCTCTTC 1 cut(s) 848
SaqAI TTAA 3 cut(s) 146, 614, 880
SatI GCNGC 5 cut(s) 105, 329, 417, 420, 582
Sau3AI GATC 9 cut(s) 32, 120, 258, 661, 692, 721, 762, 795, 808
Sau96I GGNCC 3 cut(s) 449, 534, 566
ScaI AGTACT 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 1 cut(s) 843
SfaNI GCATC 1 cut(s) 251
SinI GGWCC 2 cut(s) 449, 534
SmiMI CAYNNNNRTG 1 cut(s) 818
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 6 cut(s) 44, 147, 411, 460, 541, 754
SsiI CCGC 4 cut(s) 25, 302, 329, 416
SspI AATATT 2 cut(s) 708, 875
SspMI CTAG 4 cut(s) 96, 570, 620, 828
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 569
TaiI ACGT 2 cut(s) 297, 750
TasI AATT 6 cut(s) 44, 147, 411, 460, 541, 754
TatI WGTACW 1 cut(s) 92
TauI GCSGC 2 cut(s) 331, 419
TfiI GAWTC 2 cut(s) 249, 509
Tru1I TTAA 3 cut(s) 146, 614, 880
Tru9I TTAA 3 cut(s) 146, 614, 880
TscAI CASTG 1 cut(s) 682
TseI GCWGC 3 cut(s) 104, 419, 581
TspDTI ATGAA 5 cut(s) 140, 379, 700, 748, 798
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 1 cut(s) 682
VpaK11BI GGWCC 2 cut(s) 449, 534
XapI RAATTY 1 cut(s) 754
XbaI TCTAGA 1 cut(s) 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmaJI CCTAGG 1 cut(s) 569
XspI CTAG 4 cut(s) 96, 570, 620, 828
ZrmI AGTACT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.