Rw5G012310

salicylic acid-binding protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000171
Physical Location & Seq
Reverse (-)
14434677 .. 14435890
1214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012310.1

Sequence Viewer

Length: 813 bp
ATGATTGTGATGAAAATGGAAAATATGAGAGTGAAGCATTTTGCTTTGCCTTTTCTTGTGCTTCTTTGCTTGGCAAGAATCTGTAGTAATAGTCTTACAGCTGATCACTATCCAGACCAAATCCTCTTTGGGAAGAAGCATTTTGTGCTGGTTCATGGAGCCGGTCACGGAGCTTGGTGTTGGTATAAGTTGGCAACTCTAGTGACCTCCACAGGTCACAATGTTACAACACTAGACCTTGCAGCATCTGGGATCAACCAGATCCAGGTAGAGCAAGTTCATTTGTTCTCCGAGTACGTTGAGCCATTGATCAGTTTTCTGGGGTCTCTTACGCCAAAGGAGAGGGTTATCCTGGTTGGCCACAGCTTGGGTGGAGCCGTGATATCAATTGCCATGGAGAGGTTCCCTGAGAAAATTGCTGCTGCTGTATATGCTACTGCTGTGATGCCAGGTCCTTCTCTTAGTTACTTGACTATACATGATAAGCTCAAGGAAAGATGGGATGCTAAGCAAATGTCATCGTACCAGCTATCGCCACCAGAGGATCTAACACTAGCATTGTCATTGCTGAGACCTTCCCCAGCATTTAATGAAGATATAAAACTCACCGACGAGAAATACGGATTGGTTCCAAGAGTGTTCATCATGTGCGACCAAGACCTTACAATAGAGGAGGAAATACAAATGTGGATGATCAGGGAGAACCCACCAAATGAAGTGAAAGTGATAAATGGTTCCGATCACATGGTCATGTTCTCTAGACCACAAGAGCTGTTCTCCAACCTCCTCAAGATTGCTGAGAAATATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

270

Amino Acids

30.4

Weight (kDa)

6.06

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 46 - 170 6.9e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 47 - 155 7.2e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 48 - 258 2.7e-21 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000171)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g04550 FvH4_1g14860 FvH4_1g14860 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14870 FvH4_1g14880 FvH4_1g14881 FvH4_1g14881 FvH4_1g14891 FvH4_1g14891 FvH4_1g14891 FvH4_1g14900 FvH4_1g14900 FvH4_1g14920 FvH4_1g14930 FvH4_1g14930 FvH4_1g14930 FvH4_1g14940 FvH4_1g15260 FvH4_1g15260
malus_domestica MD00G1067600.v1.1 MD02G1091500.v1.1 MD02G1161800.v1.1 MD02G1161900.v1.1 MD02G1162000.v1.1 MD02G1162200.v1.1 MD02G1162300.v1.1 MD02G1162800.v1.1 MD02G1162900.v1.1 MD02G1163000.v1.1 MD03G1273700.v1.1
prunus_persica Prupe.7G141000_v2.0.a1 Prupe.7G141100_v2.0.a1 Prupe.7G141200_v2.0.a1 Prupe.7G141300_v2.0.a1 Prupe.7G141400_v2.0.a1 Prupe.7G141500_v2.0.a1 Prupe.7G141700_v2.0.a1
pyrus_communis pycom02g12780 pycom02g12790 pycom02g12830 pycom02g12840 pycom02g12870 pycom02g12910 pycom02g12920
rosa_chinensis RchiOBHm_Chr1g0329911 RchiOBHm_Chr1g0329921 RchiOBHm_Chr1g0329951 RchiOBHm_Chr1g0329961 RchiOBHm_Chr2g0103821 RchiOBHm_Chr2g0103831 RchiOBHm_Chr2g0103841 RchiOBHm_Chr2g0103851 RchiOBHm_Chr2g0103861 RchiOBHm_Chr2g0103871 RchiOBHm_Chr2g0103881 RchiOBHm_Chr2g0103951 RchiOBHm_Chr2g0104521
rosa_laevigata RLG00000017266 RLG00000017268 RLG00000017273 RLG00000017333 RLG00000029851 RLG00000029853
rosa_multiflora Rmu_sc0000112.1_g000007 Rmu_sc0000112.1_g000008 Rmu_sc0000112.1_g000021 Rmu_sc0001085.1_g000004 Rmu_sc0001085.1_g000007 Rmu_sc0001085.1_g000008 Rmu_sc0002081.1_g000009 Rmu_sc0002081.1_g000013 Rmu_sc0002316.1_g000050 Rmu_sc0006774.1_g000012 Rmu_sc0009241.1_g000003 Rmu_sc0013732.1_g000001 Rmu_sc0014704.1_g000001 Rmu_sc0030697.1_g000001 Rmu_ssc0000417.1_g000001 Rmu_ssc0000417.1_g000003 Rmu_ssc0000417.1_g000011 Rmu_ssc0000417.1_g000012
rosa_roxburghii Rroxscaffold_2G00138950 Rroxscaffold_2G00139230 Rroxscaffold_2G00139240 Rroxscaffold_2G00139270 Rroxscaffold_2G00139280 Rroxscaffold_2G00139320 Rroxscaffold_2G00139330 Rroxscaffold_4G00320470 Rroxscaffold_4G00320480 Rroxscaffold_4G00320500 Rroxscaffold_4G00320510
rosa_rugosa Rorug01G0084300 Rorug01G0084400.1 Rorug01G0084500 Rorug01G0084600 Rorug01G0084900 Rorug02G0117500 Rorug02G0117800 Rorug02G0117900 Rorug02G0118000 Rorug02G0118100 Rorug02G0118200 Rorug02G0118300
rosa_samantha Rh1AG102200 Rh1AG102300 Rh1AG103000 Rh1AG103300 Rh1BG081400 Rh1BG081500 Rh1BG081600 Rh1BG081700 Rh1CG097200 Rh1CG097300 Rh1CG097700 Rh1CG097800 Rh1CG098100 Rh1CG098400 Rh1DG104800 Rh1DG104900 Rh1DG105600 Rh2AG166600 Rh2AG166700 Rh2AG166800 Rh2AG167100 Rh2AG167300 Rh2AG167400 Rh2AG167500 Rh2AG167600 Rh2BG173700 Rh2BG173800 Rh2BG173900 Rh2BG174000 Rh2BG174300 Rh2BG174400 Rh2BG174500 Rh2BG174700 Rh2CG172500 Rh2CG172600 Rh2CG172800 Rh2CG172900 Rh2CG173000 Rh2CG173100 Rh2CG173200 Rh2CG173300 Rh2CG176000 Rh2DG171900 Rh2DG172000 Rh2DG172100 Rh2DG172300 Rh2DG172700 Rh2DG172800 Rh2DG172900 Rh2DG173000
rosa_wichuraiana Rw0G002040 Rw0G006890 Rw1G008090 Rw1G008100 Rw1G008140 Rw1G008150 Rw1G008200 Rw1G008300 Rw2G013050 Rw2G013060 Rw2G013080 Rw2G013090 Rw2G013100 Rw2G013390 Rw5G012310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 367
AclWI GGATC 3 cut(s) 256, 260, 552
AcoI YGGCCR 1 cut(s) 358
AfaI GTAC 2 cut(s) 296, 524
AfiI CCNNNNNNNGG 3 cut(s) 265, 367, 399
AjnI CCWGG 3 cut(s) 264, 351, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 6 cut(s) 101, 173, 366, 487, 529, 772
AluI AGCT 6 cut(s) 101, 173, 366, 487, 529, 772
Alw26I GTCTC 2 cut(s) 330, 565
AlwI GGATC 3 cut(s) 256, 260, 552
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 1 cut(s) 358
ApeKI GCWGC 3 cut(s) 242, 419, 422
AspS9I GGNCC 1 cut(s) 452
AsuHPI GGTGA 1 cut(s) 598
AvaII GGWCC 1 cut(s) 452
BalI TGGCCA 1 cut(s) 360
BbvI GCAGC 3 cut(s) 254, 406, 409
BccI CCATC 1 cut(s) 492
BceAI ACGGC 1 cut(s) 362
BciT130I CCWGG 3 cut(s) 266, 353, 450
BclI TGATCA 3 cut(s) 103, 309, 693
BcoDI GTCTC 2 cut(s) 330, 565
BfaI CTAG 4 cut(s) 200, 233, 554, 759
BfmI CTRYAG 1 cut(s) 82
BisI GCNGC 3 cut(s) 243, 420, 423
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 3 cut(s) 244, 421, 424
Bme1390I CCNGG 3 cut(s) 266, 353, 450
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 5 cut(s) 160, 376, 404, 630, 736
BmrFI CCNGG 3 cut(s) 266, 353, 450
BmsI GCATC 3 cut(s) 254, 435, 493
BplI GAGNNNNNCTC 2 cut(s) 761, 793
Bpu1102I GCTNAGC 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 473, 773
BsaBI GATNNNNATC 1 cut(s) 108
BsaI GGTCTC 2 cut(s) 330, 565
BsaJI CCNNGG 1 cut(s) 393
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 367, 399
Bse118I RCCGGY 1 cut(s) 161
Bse3DI GCAATG 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 3 cut(s) 266, 353, 450
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 2 cut(s) 508, 696
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 3 cut(s) 265, 367, 399
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 3 cut(s) 399, 560, 789
BseRI GAGGAG 2 cut(s) 686, 776
BseXI GCAGC 3 cut(s) 254, 406, 409
BseYI CCCAGC 1 cut(s) 580
BshFI GGCC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 265, 367, 399
BsmAI GTCTC 2 cut(s) 330, 565
BsnI GGCC 1 cut(s) 360
Bso31I GGTCTC 2 cut(s) 330, 565
Bsp143I GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp19I CCATGG 1 cut(s) 393
BspANI GGCC 1 cut(s) 360
BspCNI CTCAG 3 cut(s) 400, 561, 790
BspLI GGNNCC 5 cut(s) 160, 376, 404, 630, 736
BspPI GGATC 3 cut(s) 256, 260, 552
BspTNI GGTCTC 2 cut(s) 330, 565
BsrDI GCAATG 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 3 cut(s) 266, 353, 450
BstAPI GCANNNNNTGC 1 cut(s) 145
BstDEI CTNAG 5 cut(s) 408, 461, 507, 569, 798
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 2 cut(s) 508, 696
BstKTI GATC 7 cut(s) 106, 255, 264, 312, 547, 696, 742
BstMAI GTCTC 2 cut(s) 330, 565
BstMBI GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
BstMWI GCNNNNNNNGC 2 cut(s) 145, 431
BstNI CCWGG 3 cut(s) 266, 353, 450
BstSCI CCNGG 3 cut(s) 264, 351, 448
BstSFI CTRYAG 1 cut(s) 82
BstV1I GCAGC 3 cut(s) 254, 406, 409
BstX2I RGATCY 2 cut(s) 261, 544
BstYI RGATCY 2 cut(s) 261, 544
BsuRI GGCC 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 2 cut(s) 508, 696
CaiI CAGNNNCTG 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 295, 523
CviAII CATG 6 cut(s) 155, 394, 479, 646, 745, 751
CviQI GTAC 2 cut(s) 295, 523
DdeI CTNAG 5 cut(s) 408, 461, 507, 569, 798
DpnI GATC 7 cut(s) 105, 254, 263, 311, 546, 695, 741
DpnII GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
EaeI YGGCCR 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 393
Eco31I GGTCTC 2 cut(s) 330, 565
Eco32I GATATC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 452
EcoRII CCWGG 3 cut(s) 264, 351, 448
EcoRV GATATC 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 6 cut(s) 158, 397, 482, 649, 748, 754
FatI CATG 6 cut(s) 154, 393, 478, 645, 744, 750
FbaI TGATCA 3 cut(s) 103, 309, 693
Fnu4HI GCNGC 3 cut(s) 243, 420, 423
FokI GGATG 2 cut(s) 515, 703
Fsp4HI GCNGC 3 cut(s) 243, 420, 423
FspBI CTAG 4 cut(s) 200, 233, 554, 759
GluI GCNGC 3 cut(s) 243, 420, 423
GsaI CCCAGC 1 cut(s) 584
HaeIII GGCC 1 cut(s) 360
HapII CCGG 1 cut(s) 162
Hin1II CATG 6 cut(s) 158, 397, 482, 649, 748, 754
HinfI GANTC 1 cut(s) 78
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 598
Hpy188I TCNGA 2 cut(s) 292, 739
Hpy188III TCNNGA 3 cut(s) 113, 759, 790
Hpy99I CGWCG 1 cut(s) 614
HpyAV CCTTC 2 cut(s) 465, 585
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 1 cut(s) 242
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 431
HpyF3I CTNAG 5 cut(s) 408, 461, 507, 569, 798
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 6 cut(s) 158, 397, 482, 649, 748, 754
Ksp22I TGATCA 3 cut(s) 103, 309, 693
Kzo9I GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
LmnI GCTCC 3 cut(s) 158, 170, 374
Lsp1109I GCAGC 3 cut(s) 254, 406, 409
LweI GCATC 3 cut(s) 254, 435, 493
MaeI CTAG 4 cut(s) 200, 233, 554, 759
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 5 cut(s) 164, 202, 215, 223, 464
MalI GATC 7 cut(s) 105, 254, 263, 311, 546, 695, 741
MboI GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
MboII GAAGA 2 cut(s) 145, 605
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 2 cut(s) 261, 544
MlsI TGGCCA 1 cut(s) 360
MluCI AATT 2 cut(s) 387, 414
MluNI TGGCCA 1 cut(s) 360
MmeI TCCRAC 1 cut(s) 804
MnlI CCTC 9 cut(s) 134, 217, 336, 393, 535, 664, 667, 794, 797
Mox20I TGGCCA 1 cut(s) 360
MscI TGGCCA 1 cut(s) 360
MseI TTAA 1 cut(s) 588
MslI CAYNNNNRTG 1 cut(s) 749
Msp20I TGGCCA 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 3 cut(s) 266, 353, 450
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 3 cut(s) 266, 353, 450
MwoI GCNNNNNNNGC 2 cut(s) 145, 431
NcoI CCATGG 1 cut(s) 393
NdeII GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
NlaIII CATG 6 cut(s) 158, 397, 482, 649, 748, 754
NlaIV GGNNCC 5 cut(s) 160, 376, 404, 630, 736
NmuCI GTSAC 3 cut(s) 164, 202, 215
PfeI GAWTC 1 cut(s) 78
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 3 cut(s) 244, 421, 424
PpuMI RGGWCCY 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 3 cut(s) 264, 351, 448
PspFI CCCAGC 1 cut(s) 580
PspGI CCWGG 3 cut(s) 264, 351, 448
PspN4I GGNNCC 5 cut(s) 160, 376, 404, 630, 736
PspPI GGNCC 1 cut(s) 452
PspPPI RGGWCCY 1 cut(s) 452
PsrI GAACNNNNNNTAC 2 cut(s) 261, 293
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 2 cut(s) 261, 544
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 2 cut(s) 296, 524
RsaNI GTAC 2 cut(s) 295, 523
RseI CAYNNNNRTG 1 cut(s) 749
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 3 cut(s) 243, 420, 423
Sau3AI GATC 7 cut(s) 103, 252, 261, 309, 544, 693, 739
Sau96I GGNCC 1 cut(s) 452
ScrFI CCNGG 3 cut(s) 266, 353, 450
SfaNI GCATC 3 cut(s) 254, 435, 493
SfcI CTRYAG 1 cut(s) 82
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 2 cut(s) 488, 788
SmoI CTYRAG 2 cut(s) 488, 788
Sse9I AATT 2 cut(s) 387, 414
SspI AATATT 1 cut(s) 806
SspMI CTAG 4 cut(s) 200, 233, 554, 759
StyD4I CCNGG 3 cut(s) 264, 351, 448
StyI CCWWGG 1 cut(s) 393
TaiI ACGT 1 cut(s) 300
TasI AATT 2 cut(s) 387, 414
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TseFI GTSAC 3 cut(s) 164, 202, 215
TseI GCWGC 3 cut(s) 242, 419, 422
Tsp45I GTSAC 3 cut(s) 164, 202, 215
TspDTI ATGAA 7 cut(s) 26, 143, 269, 606, 631, 729, 798
TspGWI ACGGA 2 cut(s) 183, 636
Van91I CCANNNNNTGG 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 452
XbaI TCTAGA 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 2 cut(s) 125, 368
XspI CTAG 4 cut(s) 200, 233, 554, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.