FvH4_1g29150

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000298
Physical Location & Seq
Reverse (-)
21804200 .. 21807706
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29150.t2

Sequence Viewer

Length: 1275 bp
ATGCCAAGAAAAAAGAATCCCAGGAAAAAAGGAAAATGGAAACCCAAATGTGCTTTCGATATGGAGCAACTTCCTCAAGCTCTAGTAATGGACATTCTTTCAAGGCTTCCAATCAAGAGCCTCTGCAATTGCAAATACGTTTGCAAAGAATGGCTTCACATGATTCATGGCCCTGAATTTGCTCGGCTACACCTTCAAAGATCACCCATCAGCATCTTGATCAAGACCTCGCCTCCTAGAAGAGAACAAAGCAAACTTGATTTAATCCATGTAGAAAGTGATGGGTCTGATATGCGAGTGGACAGAATGAGATTTGCTCCCCTGAATAGCATACCTTGCTATCTTTTTCAAGTGGATTTGATGAATTCATGCAATGGTCTCCTTTGTTTGACTGAGTCTAAGCGAGATGGTTCCTTGTATGTGTGCAATCCTATATTGGGTCAGTACATTAGCATTCCTTGTCCTGATAAGAATAGATATTACTCTTGCAATTTTACGGCAATTGGTTTTAGCGCCAAGACTAAGGAATACAAGGTGGTGCAAACAAGTTTCAGGGAATACAACCGGAGTGAGCCTGTGGCTCACATATACACCATTGGCACAGGAGTTTGGAGAAGTCTTGGAAAGCCCCCTAGAGGTGGCCGAATTCCATTTGATTCTTTTCTGCATGGAACTCTTCATTGGATTCCCTATGCAAGCTATAACTATACTCCTCAATCTATACAATCTTTTGACTTTGAAAGAGAAGAGTTTCGACCACTACCATTGCCCCCAGTAAGCCTGAAGAAATCCGGCCAATATGATGGTTTCGGGTTGGGAGTCTTGAAAGGTTGTATTTTATCATGTGTGTTTGAAAGTAATCAATGTAAGGGTAGGAGTCAAGGGACAGTTGACATGTGGATTATGAAGGATTATGGTGTTCAAGAGTCTTGGACCAAAATTCTTGTCATCGAAAACTTGTATCCAGACAGTTCATATAAACCAATTATGTTCTTGAATGATGTGGAAATCCTAATGAGGTACAGACGGTCTGTGGTATACTATGATCAAGAAACAAAAAGTCTCAAACGAACTCGCATTGTTCAGACTCAGTCTGAGTTTGAGGCATTTGGTTTTTGTCCTTGTTTTGTTTCACTCCATGATGTTTCAAAAGGAGAGGAGGTGAAAAGGATAAGAGACAAGCATCAAGCTTACAATATCCTGCTGGGTAGAGGGAGCAGTGATAGTGCTTGTTCCAGCGTGCCACCTAATCACAAGTCCAAAAAGCTTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

425

Amino Acids

48.8

Weight (kDa)

9.28

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 60 2.8e-08 F-box domain
F-box-like PF12937 22 - 58 9.1e-08 F-box-like
FBA_1 PF07734 115 - 364 4.6e-30 F-box associated beta propeller domain
FBA_3 PF08268 117 - 367 9.9e-21 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 120 - 263 6.7e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29150 FvH4_1g29150 FvH4_2g35201 FvH4_3g36371 FvH4_4g12590 FvH4_4g12590 FvH4_4g13903 FvH4_4g16630 FvH4_4g16630 FvH4_4g16640 FvH4_4g16640 FvH4_4g16640
malus_domestica MD03G1174100.v1.1 MD13G1177900.v1.1 MD13G1178000.v1.1 MD13G1178100.v1.1
prunus_persica Prupe.1G146800_v2.0.a1 Prupe.1G146900_v2.0.a1 Prupe.1G147000_v2.0.a1 Prupe.1G147100_v2.0.a1 Prupe.1G147200_v2.0.a1 Prupe.1G185600_v2.0.a1 Prupe.1G529300_v2.0.a1 Prupe.6G154200_v2.0.a1 Prupe.6G217100_v2.0.a1
pyrus_communis pycom03g13170 pycom03g13180 pycom05g29500 pycom13g15350 pycom13g15380 pycom13g15390 pycom13g15400
rosa_chinensis RchiOBHm_Chr4g0395601 RchiOBHm_Chr4g0395611 RchiOBHm_Chr4g0395621 RchiOBHm_Chr4g0413281 RchiOBHm_Chr4g0419621 RchiOBHm_Chr4g0419631 RchiOBHm_Chr4g0419901 RchiOBHm_Chr4g0419911 RchiOBHm_Chr4g0419981 RchiOBHm_Chr4g0420081 RchiOBHm_Chr4g0420091 RchiOBHm_Chr6g0306731
rosa_laevigata RLG00000006686 RLG00000007789 RLG00000007793 RLG00000007794 RLG00000007808 RLG00000007809 RLG00000011318
rosa_multiflora Rmu_co8471007.1_g000001 Rmu_sc0000717.1_g000001 Rmu_sc0003141.1_g000001 Rmu_sc0006133.1_g000013 Rmu_sc0013209.1_g000001 Rmu_sc0013209.1_g000002 Rmu_sc0017785.1_g000004 Rmu_sc0017786.1_g000003 Rmu_sc0023895.1_g000004 Rmu_sc0032897.1_g000004
rosa_roxburghii Rroxscaffold_5G00339000 Rroxscaffold_5G00357390 Rroxscaffold_5G00362460 Rroxscaffold_5G00362470 Rroxscaffold_5G00362730 Rroxscaffold_5G00362740 Rroxscaffold_5G00362800
rosa_rugosa Rorug04G0158700 Rorug04G0158800 Rorug04G0158800 Rorug04G0158900 Rorug04G0159000 Rorug04G0159100 Rorug04G0159200 Rorug04G0159300 Rorug04G0161200 Rorug04G0161300 Rorug04G0161300 Rorug04G0161400 Rorug04G0162000 Rorug04G0162100
rosa_samantha Rh1BG049900 Rh4AG179100 Rh4AG179200 Rh4AG220700 Rh4AG222300 Rh4AG222400 Rh4AG222700 Rh4AG222900 Rh4BG222400 Rh4BG222600 Rh4BG224700 Rh4BG224800 Rh4BG225300 Rh4BG225400 Rh4BG338700 Rh4BG338800 Rh4CG235500 Rh4DG174300 Rh4DG219400 Rh4DG219600 Rh4DG221300 Rh4DG221400 Rh4DG221700 Rh5CG465500 Rh5DG455700 Rh6BG417700 Rh6BG436500 Rh6BG436600 Rh6CG478500 Rh6CG478600 Rh6DG465600 Rh6DG465700 Rh6DG492000
rosa_wichuraiana Rw4G018980 Rw4G019160 Rw4G019170 Rw4G019190 Rw4G028680 Rw5G040160 Rw6G040460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1038
AcoI YGGCCR 2 cut(s) 640, 793
AcsI RAATTY 4 cut(s) 176, 364, 645, 939
AcuI CTGAAG 1 cut(s) 803
AfaI GTAC 2 cut(s) 446, 1022
AfiI CCNNNNNNNGG 3 cut(s) 437, 635, 638
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 9 cut(s) 102, 197, 350, 740, 826, 854, 923, 997, 1149
AjnI CCWGG 1 cut(s) 20
AjuI GAANNNNNNNTTGG 4 cut(s) 38, 70, 664, 696
AluBI AGCT 4 cut(s) 80, 699, 1190, 1267
AluI AGCT 4 cut(s) 80, 699, 1190, 1267
Alw26I GTCTC 3 cut(s) 383, 1067, 1170
AoxI GGCC 3 cut(s) 169, 640, 793
ApoI RAATTY 4 cut(s) 176, 364, 645, 939
ArsI GACNNNNNNTTYG 2 cut(s) 930, 962
Asp700I GAANNNNTTC 2 cut(s) 153, 750
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 2 cut(s) 170, 933
AsuHPI GGTGA 2 cut(s) 195, 1174
AvaII GGWCC 1 cut(s) 933
BccI CCATC 4 cut(s) 215, 275, 401, 797
BceAI ACGGC 1 cut(s) 513
BciT130I CCWGG 1 cut(s) 22
BciVI GTATCC 1 cut(s) 972
BclI TGATCA 2 cut(s) 219, 1045
BcoDI GTCTC 3 cut(s) 383, 1067, 1170
BfaI CTAG 3 cut(s) 83, 237, 633
BfoI RGCGCY 1 cut(s) 516
BfuI GTATCC 1 cut(s) 972
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 1 cut(s) 933
BmgT120I GGNCC 2 cut(s) 170, 933
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 1 cut(s) 22
BmrI ACTGGG 1 cut(s) 767
BmsI GCATC 2 cut(s) 222, 1192
BmuI ACTGGG 1 cut(s) 767
BplI GAGNNNNNCTC 2 cut(s) 301, 333
BpuEI CTTGAG 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 20
BsaWI WCCGGW 1 cut(s) 564
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 1146, 1176
Bsc4I CCNNNNNNNGG 3 cut(s) 437, 635, 638
Bse1I ACTGG 1 cut(s) 773
Bse3DI GCAATG 2 cut(s) 379, 764
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 20
BseLI CCNNNNNNNGG 3 cut(s) 437, 635, 638
BseMI GCAATG 2 cut(s) 379, 764
BseMII CTCAG 3 cut(s) 384, 1086, 1103
BseNI ACTGG 1 cut(s) 773
BseRI GAGGAG 2 cut(s) 702, 1172
BseYI CCCAGC 1 cut(s) 1204
BshFI GGCC 3 cut(s) 171, 642, 795
BsiSI CCGG 2 cut(s) 565, 792
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 3 cut(s) 437, 635, 638
BsmAI GTCTC 3 cut(s) 383, 1067, 1170
BsmFI GGGAC 1 cut(s) 898
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 3 cut(s) 171, 642, 795
Bso31I GGTCTC 1 cut(s) 383
Bsp143I GATC 3 cut(s) 200, 219, 1045
BspANI GGCC 3 cut(s) 171, 642, 795
BspCNI CTCAG 3 cut(s) 385, 1087, 1102
BspLI GGNNCC 1 cut(s) 412
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 379, 764
BsrI ACTGG 1 cut(s) 773
BssECI CCNNGG 1 cut(s) 20
BssMI GATC 3 cut(s) 200, 219, 1045
BssNAI GTATAC 1 cut(s) 1039
Bst1107I GTATAC 1 cut(s) 1039
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 889, 971, 1029
Bst6I CTCTTC 3 cut(s) 235, 681, 741
BstAPI GCANNNNNTGC 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 697, 1241
BstDEI CTNAG 5 cut(s) 393, 399, 522, 1089, 1095
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 3 cut(s) 203, 222, 1048
BstMAI GTCTC 3 cut(s) 383, 1067, 1170
BstMBI GATC 3 cut(s) 200, 219, 1045
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNI CCWGG 1 cut(s) 22
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 1 cut(s) 20
BstXI CCANNNNNNTGG 1 cut(s) 803
BstZ17I GTATAC 1 cut(s) 1039
BsuI GTATCC 1 cut(s) 972
BsuRI GGCC 3 cut(s) 171, 642, 795
BtsI GCAGTG 1 cut(s) 1225
BtsIMutI CAGTG 1 cut(s) 1225
Cac8I GCNNGC 2 cut(s) 697, 1241
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 2 cut(s) 170, 933
Csp6I GTAC 2 cut(s) 445, 1021
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 8 cut(s) 160, 167, 269, 369, 668, 843, 895, 1139
CviQI GTAC 2 cut(s) 445, 1021
DdeI CTNAG 5 cut(s) 393, 399, 522, 1089, 1095
DpnI GATC 3 cut(s) 202, 221, 1047
DpnII GATC 3 cut(s) 200, 219, 1045
EaeI YGGCCR 2 cut(s) 640, 793
Eam1104I CTCTTC 3 cut(s) 235, 681, 741
EarI CTCTTC 3 cut(s) 235, 681, 741
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 933
Eco57I CTGAAG 1 cut(s) 803
EcoRI GAATTC 2 cut(s) 364, 645
EcoRII CCWGG 1 cut(s) 20
FaeI CATG 8 cut(s) 163, 170, 272, 372, 671, 846, 898, 1142
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 1 cut(s) 898
FatI CATG 8 cut(s) 159, 166, 268, 368, 667, 842, 894, 1138
FbaI TGATCA 2 cut(s) 219, 1045
FblI GTMKAC 1 cut(s) 1038
FspBI CTAG 3 cut(s) 83, 237, 633
GlaI GCGC 1 cut(s) 514
GsaI CCCAGC 1 cut(s) 1208
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 3 cut(s) 171, 642, 795
HapII CCGG 2 cut(s) 565, 792
HhaI GCGC 1 cut(s) 515
Hin1II CATG 8 cut(s) 163, 170, 272, 372, 671, 846, 898, 1142
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 2 cut(s) 1188, 1265
HinfI GANTC 9 cut(s) 16, 163, 395, 656, 685, 819, 877, 926, 1087
HpaII CCGG 2 cut(s) 565, 792
HphI GGTGA 2 cut(s) 195, 1174
Hpy166II GTNNAC 3 cut(s) 301, 892, 1039
Hpy188I TCNGA 3 cut(s) 289, 1086, 1096
Hpy188III TCNNGA 9 cut(s) 115, 217, 223, 464, 823, 923, 965, 994, 1049
Hpy8I GTNNAC 3 cut(s) 301, 892, 1039
HpyAV CCTTC 2 cut(s) 203, 901
HpyCH4III ACNGT 3 cut(s) 889, 971, 1029
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 9 cut(s) 126, 132, 144, 372, 426, 489, 541, 667, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 5 cut(s) 393, 399, 522, 1089, 1095
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 8 cut(s) 163, 170, 272, 372, 671, 846, 898, 1142
HspAI GCGC 1 cut(s) 513
Ksp22I TGATCA 2 cut(s) 219, 1045
Kzo9I GATC 3 cut(s) 200, 219, 1045
LmnI GCTCC 3 cut(s) 64, 322, 1215
LweI GCATC 2 cut(s) 222, 1192
MaeI CTAG 3 cut(s) 83, 237, 633
MaeII ACGT 1 cut(s) 138
MalI GATC 3 cut(s) 202, 221, 1047
MboI GATC 3 cut(s) 200, 219, 1045
MboII GAAGA 4 cut(s) 252, 668, 758, 796
MfeI CAATTG 2 cut(s) 127, 501
MluCI AATT 8 cut(s) 127, 176, 364, 490, 501, 645, 939, 984
MlyI GAGTC 5 cut(s) 404, 828, 886, 935, 1081
MroXI GAANNNNTTC 2 cut(s) 153, 750
MseI TTAA 2 cut(s) 263, 1269
MspI CCGG 2 cut(s) 565, 792
MspR9I CCNGG 1 cut(s) 22
MunI CAATTG 2 cut(s) 127, 501
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 1 cut(s) 22
MwoI GCNNNNNNNGC 1 cut(s) 336
NdeII GATC 3 cut(s) 200, 219, 1045
NlaIII CATG 8 cut(s) 163, 170, 272, 372, 671, 846, 898, 1142
NlaIV GGNNCC 1 cut(s) 412
NmeAIII GCCGAG 1 cut(s) 163
NspI RCATGY 1 cut(s) 898
PciI ACATGT 1 cut(s) 894
PctI GAATGC 1 cut(s) 453
PdmI GAANNNNTTC 2 cut(s) 153, 750
PfeI GAWTC 4 cut(s) 16, 163, 656, 685
PflFI GACNNNGTC 2 cut(s) 394, 1090
PleI GAGTC 5 cut(s) 403, 827, 885, 934, 1081
PpsI GAGTC 5 cut(s) 403, 827, 885, 934, 1081
PscI ACATGT 1 cut(s) 894
Psp6I CCWGG 1 cut(s) 20
PspFI CCCAGC 1 cut(s) 1204
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 412
PspPI GGNCC 2 cut(s) 170, 933
PsyI GACNNNGTC 2 cut(s) 394, 1090
RsaI GTAC 2 cut(s) 446, 1022
RsaNI GTAC 2 cut(s) 445, 1021
SaqAI TTAA 2 cut(s) 263, 1269
Sau3AI GATC 3 cut(s) 200, 219, 1045
Sau96I GGNCC 2 cut(s) 170, 933
SchI GAGTC 5 cut(s) 404, 828, 886, 935, 1081
ScrFI CCNGG 1 cut(s) 22
SfaNI GCATC 2 cut(s) 222, 1192
SinI GGWCC 1 cut(s) 933
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 8 cut(s) 127, 176, 364, 490, 501, 645, 939, 984
SspMI CTAG 3 cut(s) 83, 237, 633
StyD4I CCNGG 1 cut(s) 20
TaaI ACNGT 3 cut(s) 889, 971, 1029
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 57, 754, 951
TasI AATT 8 cut(s) 127, 176, 364, 490, 501, 645, 939, 984
TatI WGTACW 1 cut(s) 444
TfiI GAWTC 4 cut(s) 16, 163, 656, 685
Tru1I TTAA 2 cut(s) 263, 1269
Tru9I TTAA 2 cut(s) 263, 1269
TscAI CASTG 1 cut(s) 1225
TspDTI ATGAA 6 cut(s) 155, 357, 377, 668, 920, 963
TspRI CASTG 1 cut(s) 1225
Tth111I GACNNNGTC 2 cut(s) 394, 1090
VpaK11BI GGWCC 1 cut(s) 933
XapI RAATTY 4 cut(s) 176, 364, 645, 939
XceI RCATGY 1 cut(s) 898
XmiI GTMKAC 1 cut(s) 1038
XmnI GAANNNNTTC 2 cut(s) 153, 750
XspI CTAG 3 cut(s) 83, 237, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.