F-box-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 804, 1050 |
| Acc36I | ACCTGC | 1 cut(s) | 506 |
| Acc65I | GGTACC | 1 cut(s) | 652 |
| AccB1I | GGYRCC | 1 cut(s) | 652 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 956 |
| AcoI | YGGCCR | 2 cut(s) | 574, 958 |
| AcsI | RAATTY | 4 cut(s) | 385, 872, 924, 1193 |
| AcuI | CTGAAG | 3 cut(s) | 390, 833, 1292 |
| AcyI | GRCGYC | 1 cut(s) | 153 |
| AfaI | GTAC | 4 cut(s) | 204, 548, 601, 654 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 455, 776, 956, 1039 |
| AflIII | ACRYGT | 1 cut(s) | 879 |
| AjnI | CCWGG | 2 cut(s) | 35, 533 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 53, 85 |
| AluBI | AGCT | 3 cut(s) | 680, 1221, 1250 |
| AluI | AGCT | 3 cut(s) | 680, 1221, 1250 |
| Alw21I | GWGCWC | 2 cut(s) | 682, 862 |
| Alw26I | GTCTC | 5 cut(s) | 262, 842, 1075, 1104, 1176 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1283 |
| AoxI | GGCC | 3 cut(s) | 132, 574, 958 |
| ApoI | RAATTY | 4 cut(s) | 385, 872, 924, 1193 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 176, 208 |
| AseI | ATTAAT | 1 cut(s) | 278 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 750 |
| Asp718I | GGTACC | 1 cut(s) | 652 |
| AspS9I | GGNCC | 4 cut(s) | 133, 240, 537, 1030 |
| AsuC2I | CCSGG | 1 cut(s) | 1034 |
| AsuHPI | GGTGA | 4 cut(s) | 210, 470, 1189, 1313 |
| AvaII | GGWCC | 3 cut(s) | 240, 537, 1030 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 591, 624 |
| BalI | TGGCCA | 2 cut(s) | 576, 960 |
| BanI | GGYRCC | 1 cut(s) | 652 |
| BanII | GRGCYC | 1 cut(s) | 682 |
| Bbv12I | GWGCWC | 2 cut(s) | 682, 862 |
| BccI | CCATC | 5 cut(s) | 134, 230, 422, 998, 1298 |
| BciT130I | CCWGG | 2 cut(s) | 37, 535 |
| BciVI | GTATCC | 1 cut(s) | 957 |
| BclI | TGATCA | 2 cut(s) | 234, 1252 |
| BcnI | CCSGG | 1 cut(s) | 1034 |
| BcoDI | GTCTC | 5 cut(s) | 262, 842, 1075, 1104, 1176 |
| BfaI | CTAG | 3 cut(s) | 6, 117, 633 |
| BfuAI | ACCTGC | 1 cut(s) | 506 |
| BfuI | GTATCC | 1 cut(s) | 957 |
| Bme1390I | CCNGG | 3 cut(s) | 37, 535, 1034 |
| Bme18I | GGWCC | 3 cut(s) | 240, 537, 1030 |
| BmgT120I | GGNCC | 4 cut(s) | 133, 240, 537, 1030 |
| BmiI | GGNNCC | 5 cut(s) | 630, 654, 693, 1237, 1309 |
| BmrFI | CCNGG | 3 cut(s) | 37, 535, 1034 |
| BmrI | ACTGGG | 1 cut(s) | 1089 |
| BmsI | GCATC | 1 cut(s) | 352 |
| BmuI | ACTGGG | 1 cut(s) | 1089 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 319, 351 |
| Bpu10I | CCTNAGC | 1 cut(s) | 414 |
| BpuMI | CCSGG | 1 cut(s) | 1034 |
| BsaHI | GRCGYC | 1 cut(s) | 153 |
| BsaJI | CCNNGG | 3 cut(s) | 35, 648, 950 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1152, 1182 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 455, 776, 956, 1039 |
| Bse1I | ACTGG | 5 cut(s) | 56, 170, 353, 577, 1084 |
| Bse3DI | GCAATG | 1 cut(s) | 400 |
| BseBI | CCWGG | 2 cut(s) | 37, 535 |
| BseDI | CCNNGG | 3 cut(s) | 35, 648, 950 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 455, 776, 956, 1039 |
| BseMI | GCAATG | 1 cut(s) | 400 |
| BseMII | CTCAG | 3 cut(s) | 573, 728, 1109 |
| BseNI | ACTGG | 5 cut(s) | 56, 170, 353, 577, 1084 |
| BseRI | GAGGAG | 1 cut(s) | 1178 |
| BshFI | GGCC | 3 cut(s) | 134, 576, 960 |
| BshNI | GGYRCC | 1 cut(s) | 652 |
| BsiHKAI | GWGCWC | 2 cut(s) | 682, 862 |
| BsiSI | CCGG | 1 cut(s) | 1033 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 455, 776, 956, 1039 |
| BsmAI | GTCTC | 5 cut(s) | 262, 842, 1075, 1104, 1176 |
| BsmI | GAATGC | 1 cut(s) | 471 |
| BsnI | GGCC | 3 cut(s) | 134, 576, 960 |
| Bsp1286I | GDGCHC | 2 cut(s) | 682, 862 |
| Bsp1407I | TGTACA | 1 cut(s) | 202 |
| Bsp143I | GATC | 3 cut(s) | 215, 234, 1252 |
| Bsp19I | CCATGG | 2 cut(s) | 648, 950 |
| BspANI | GGCC | 3 cut(s) | 134, 576, 960 |
| BspCNI | CTCAG | 3 cut(s) | 574, 727, 1108 |
| BspLI | GGNNCC | 5 cut(s) | 630, 654, 693, 1237, 1309 |
| BspMI | ACCTGC | 1 cut(s) | 506 |
| BspQI | GCTCTTC | 1 cut(s) | 687 |
| BspT107I | GGYRCC | 1 cut(s) | 652 |
| BsrDI | GCAATG | 1 cut(s) | 400 |
| BsrGI | TGTACA | 1 cut(s) | 202 |
| BsrI | ACTGG | 5 cut(s) | 56, 170, 353, 577, 1084 |
| BssECI | CCNNGG | 3 cut(s) | 35, 648, 950 |
| BssMI | GATC | 3 cut(s) | 215, 234, 1252 |
| BssNI | GRCGYC | 1 cut(s) | 153 |
| BssT1I | CCWWGG | 2 cut(s) | 648, 950 |
| Bst2UI | CCWGG | 2 cut(s) | 37, 535 |
| Bst4CI | ACNGT | 5 cut(s) | 209, 657, 710, 726, 1069 |
| Bst6I | CTCTTC | 3 cut(s) | 687, 845, 1194 |
| BstACI | GRCGYC | 1 cut(s) | 153 |
| BstAUI | TGTACA | 1 cut(s) | 202 |
| BstC8I | GCNNGC | 2 cut(s) | 301, 1279 |
| BstDEI | CTNAG | 5 cut(s) | 212, 414, 582, 714, 1095 |
| BstDSI | CCRYGG | 2 cut(s) | 648, 950 |
| BstKTI | GATC | 3 cut(s) | 218, 237, 1255 |
| BstMAI | GTCTC | 5 cut(s) | 262, 842, 1075, 1104, 1176 |
| BstMBI | GATC | 3 cut(s) | 215, 234, 1252 |
| BstNI | CCWGG | 2 cut(s) | 37, 535 |
| BstNSI | RCATGY | 1 cut(s) | 883 |
| BstSCI | CCNGG | 3 cut(s) | 35, 533, 1032 |
| BsuI | GTATCC | 1 cut(s) | 957 |
| BsuRI | GGCC | 3 cut(s) | 134, 576, 960 |
| BtgI | CCRYGG | 2 cut(s) | 648, 950 |
| BtsIMutI | CAGTG | 1 cut(s) | 205 |
| BveI | ACCTGC | 1 cut(s) | 506 |
| Cac8I | GCNNGC | 2 cut(s) | 301, 1279 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1283 |
| Cfr13I | GGNCC | 4 cut(s) | 133, 240, 537, 1030 |
| CseI | GACGC | 1 cut(s) | 142 |
| Csp6I | GTAC | 4 cut(s) | 203, 547, 600, 653 |
| CviAII | CATG | 7 cut(s) | 390, 649, 674, 880, 951, 962, 1145 |
| CviQI | GTAC | 4 cut(s) | 203, 547, 600, 653 |
| DdeI | CTNAG | 5 cut(s) | 212, 414, 582, 714, 1095 |
| DpnI | GATC | 3 cut(s) | 217, 236, 1254 |
| DpnII | GATC | 3 cut(s) | 215, 234, 1252 |
| EaeI | YGGCCR | 2 cut(s) | 574, 958 |
| Eam1104I | CTCTTC | 3 cut(s) | 687, 845, 1194 |
| EarI | CTCTTC | 3 cut(s) | 687, 845, 1194 |
| Ecl136II | GAGCTC | 1 cut(s) | 680 |
| Eco130I | CCWWGG | 2 cut(s) | 648, 950 |
| Eco24I | GRGCYC | 1 cut(s) | 682 |
| Eco47I | GGWCC | 3 cut(s) | 240, 537, 1030 |
| Eco53kI | GAGCTC | 1 cut(s) | 680 |
| Eco57I | CTGAAG | 3 cut(s) | 390, 833, 1292 |
| EcoICRI | GAGCTC | 1 cut(s) | 680 |
| EcoO109I | RGGNCCY | 1 cut(s) | 133 |
| EcoRII | CCWGG | 2 cut(s) | 35, 533 |
| EcoT14I | CCWWGG | 2 cut(s) | 648, 950 |
| EcoT22I | ATGCAT | 1 cut(s) | 979 |
| EcoT38I | GRGCYC | 1 cut(s) | 682 |
| ErhI | CCWWGG | 2 cut(s) | 648, 950 |
| FaeI | CATG | 7 cut(s) | 393, 652, 677, 883, 954, 965, 1148 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 153, 185 |
| FatI | CATG | 7 cut(s) | 389, 648, 673, 879, 950, 961, 1144 |
| FauNDI | CATATG | 1 cut(s) | 979 |
| FbaI | TGATCA | 2 cut(s) | 234, 1252 |
| FriOI | GRGCYC | 1 cut(s) | 682 |
| FspBI | CTAG | 3 cut(s) | 6, 117, 633 |
| HaeIII | GGCC | 3 cut(s) | 134, 576, 960 |
| HapII | CCGG | 1 cut(s) | 1033 |
| HgaI | GACGC | 1 cut(s) | 142 |
| Hin1I | GRCGYC | 1 cut(s) | 153 |
| Hin1II | CATG | 7 cut(s) | 393, 652, 677, 883, 954, 965, 1148 |
| HincII | GTYRAC | 1 cut(s) | 1289 |
| HindII | GTYRAC | 1 cut(s) | 1289 |
| HinfI | GANTC | 2 cut(s) | 31, 911 |
| HpaII | CCGG | 1 cut(s) | 1033 |
| HphI | GGTGA | 4 cut(s) | 210, 470, 1189, 1313 |
| Hpy166II | GTNNAC | 2 cut(s) | 205, 1289 |
| Hpy188I | TCNGA | 4 cut(s) | 361, 370, 583, 813 |
| Hpy188III | TCNNGA | 6 cut(s) | 238, 283, 482, 1055, 1074, 1101 |
| Hpy8I | GTNNAC | 2 cut(s) | 205, 1289 |
| HpyAV | CCTTC | 3 cut(s) | 486, 886, 1267 |
| HpyCH4III | ACNGT | 5 cut(s) | 209, 657, 710, 726, 1069 |
| HpyF3I | CTNAG | 5 cut(s) | 212, 414, 582, 714, 1095 |
| Hsp92I | GRCGYC | 1 cut(s) | 153 |
| Hsp92II | CATG | 7 cut(s) | 393, 652, 677, 883, 954, 965, 1148 |
| KpnI | GGTACC | 1 cut(s) | 656 |
| Ksp22I | TGATCA | 2 cut(s) | 234, 1252 |
| Kzo9I | GATC | 3 cut(s) | 215, 234, 1252 |
| LguI | GCTCTTC | 1 cut(s) | 687 |
| LmnI | GCTCC | 4 cut(s) | 340, 634, 677, 1218 |
| LweI | GCATC | 1 cut(s) | 352 |
| MaeI | CTAG | 3 cut(s) | 6, 117, 633 |
| MaeIII | GTNAC | 1 cut(s) | 182 |
| MalI | GATC | 3 cut(s) | 217, 236, 1254 |
| MboI | GATC | 3 cut(s) | 215, 234, 1252 |
| MboII | GAAGA | 4 cut(s) | 674, 832, 988, 1211 |
| MfeI | CAATTG | 2 cut(s) | 142, 1113 |
| MhlI | GDGCHC | 2 cut(s) | 682, 862 |
| MlsI | TGGCCA | 2 cut(s) | 576, 960 |
| MluNI | TGGCCA | 2 cut(s) | 576, 960 |
| MlyI | GAGTC | 1 cut(s) | 920 |
| MmeI | TCCRAC | 1 cut(s) | 496 |
| MnlI | CCTC | 6 cut(s) | 38, 198, 321, 733, 1156, 1159 |
| Mox20I | TGGCCA | 2 cut(s) | 576, 960 |
| Mph1103I | ATGCAT | 1 cut(s) | 979 |
| MroXI | GAANNNNTTC | 1 cut(s) | 750 |
| MscI | TGGCCA | 2 cut(s) | 576, 960 |
| MseI | TTAA | 3 cut(s) | 278, 528, 827 |
| MslI | CAYNNNNRTG | 1 cut(s) | 867 |
| Msp20I | TGGCCA | 2 cut(s) | 576, 960 |
| MspA1I | CMGCKG | 1 cut(s) | 1250 |
| MspI | CCGG | 1 cut(s) | 1033 |
| MspR9I | CCNGG | 3 cut(s) | 37, 535, 1034 |
| MunI | CAATTG | 2 cut(s) | 142, 1113 |
| Mva1269I | GAATGC | 1 cut(s) | 471 |
| MvaI | CCWGG | 2 cut(s) | 37, 535 |
| NciI | CCSGG | 1 cut(s) | 1034 |
| NcoI | CCATGG | 2 cut(s) | 648, 950 |
| NdeI | CATATG | 1 cut(s) | 979 |
| NdeII | GATC | 3 cut(s) | 215, 234, 1252 |
| NlaIII | CATG | 7 cut(s) | 393, 652, 677, 883, 954, 965, 1148 |
| NlaIV | GGNNCC | 5 cut(s) | 630, 654, 693, 1237, 1309 |
| NmuCI | GTSAC | 1 cut(s) | 182 |
| NsiI | ATGCAT | 1 cut(s) | 979 |
| NspI | RCATGY | 1 cut(s) | 883 |
| PciI | ACATGT | 1 cut(s) | 879 |
| PciSI | GCTCTTC | 1 cut(s) | 687 |
| PctI | GAATGC | 1 cut(s) | 471 |
| PdmI | GAANNNNTTC | 1 cut(s) | 750 |
| PfeI | GAWTC | 1 cut(s) | 31 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 956 |
| PleI | GAGTC | 1 cut(s) | 919 |
| PpsI | GAGTC | 1 cut(s) | 919 |
| PscI | ACATGT | 1 cut(s) | 879 |
| PshBI | ATTAAT | 1 cut(s) | 278 |
| PsiI | TTATAA | 2 cut(s) | 804, 1050 |
| Psp124BI | GAGCTC | 1 cut(s) | 682 |
| Psp6I | CCWGG | 2 cut(s) | 35, 533 |
| PspGI | CCWGG | 2 cut(s) | 35, 533 |
| PspN4I | GGNNCC | 5 cut(s) | 630, 654, 693, 1237, 1309 |
| PspPI | GGNCC | 4 cut(s) | 133, 240, 537, 1030 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1283 |
| PvuII | CAGCTG | 1 cut(s) | 1250 |
| RsaI | GTAC | 4 cut(s) | 204, 548, 601, 654 |
| RsaNI | GTAC | 4 cut(s) | 203, 547, 600, 653 |
| RseI | CAYNNNNRTG | 1 cut(s) | 867 |
| SacI | GAGCTC | 1 cut(s) | 682 |
| SapI | GCTCTTC | 1 cut(s) | 687 |
| SaqAI | TTAA | 3 cut(s) | 278, 528, 827 |
| Sau3AI | GATC | 3 cut(s) | 215, 234, 1252 |
| Sau96I | GGNCC | 4 cut(s) | 133, 240, 537, 1030 |
| SchI | GAGTC | 1 cut(s) | 920 |
| ScrFI | CCNGG | 3 cut(s) | 37, 535, 1034 |
| SduI | GDGCHC | 2 cut(s) | 682, 862 |
| SfaNI | GCATC | 1 cut(s) | 352 |
| SinI | GGWCC | 3 cut(s) | 240, 537, 1030 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 867 |
| SspMI | CTAG | 3 cut(s) | 6, 117, 633 |
| SstI | GAGCTC | 1 cut(s) | 682 |
| StyD4I | CCNGG | 3 cut(s) | 35, 533, 1032 |
| StyI | CCWWGG | 2 cut(s) | 648, 950 |
| TaaI | ACNGT | 5 cut(s) | 209, 657, 710, 726, 1069 |
| TaqI | TCGA | 2 cut(s) | 754, 1102 |
| TatI | WGTACW | 2 cut(s) | 202, 546 |
| TfiI | GAWTC | 1 cut(s) | 31 |
| Tru1I | TTAA | 3 cut(s) | 278, 528, 827 |
| Tru9I | TTAA | 3 cut(s) | 278, 528, 827 |
| TscAI | CASTG | 1 cut(s) | 212 |
| TseFI | GTSAC | 1 cut(s) | 182 |
| Tsp45I | GTSAC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 6 cut(s) | 44, 378, 496, 674, 905, 996 |
| TspRI | CASTG | 1 cut(s) | 212 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 956 |
| VpaK11BI | GGWCC | 3 cut(s) | 240, 537, 1030 |
| VspI | ATTAAT | 1 cut(s) | 278 |
| XapI | RAATTY | 4 cut(s) | 385, 872, 924, 1193 |
| XceI | RCATGY | 1 cut(s) | 883 |
| XmnI | GAANNNNTTC | 1 cut(s) | 750 |
| XspI | CTAG | 3 cut(s) | 6, 117, 633 |
| Zsp2I | ATGCAT | 1 cut(s) | 979 |