Rroxscaffold_5G00362460

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000298
Physical Location & Seq
Reverse (-)
43465894 .. 43468766
2873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362460.1

Sequence Viewer

Length: 1302 bp
ATGCCTATCAATCCCAGGAAAAGAGGAAAATGGAAACACAAAGGTGCTTTCAATATACAGGAACTTCCACAAGCTTTAGTAATGGACATTCTATCTAGGCTTTCCATCAAGGCCCTTTACAATTGTAGGTGCGTCTGCAAAGACTGGCTTCACATAATTTGTGACCCTCTATTTGCTAATGTACACCGTCAAAGATCACCCATTGGCATATTGATCAGGACCAATCCCCGTGCAGAAAAACAAAGGAAACTTAGATTAGTCCAGATAGAAAAATGTGCCGGCTCTCGTACGCGAATCGAAAGAATGAGGTTTGCTCCCCAAAATAGCCTACCAGTGTCCGCTGCTTCTGAAGTTATGCTGATAAATTCATGCAATGGATTAGTTTGTTTGGCTAAGGGGCCTGTTGTAGATGGTGCCTTGTTTGTATGCAATCCAATTTTGGGCGAGTACATTAGCATTCCTTCTCCTGATAAGAGTATTATGGCCTACTGGTTCAAATTTGTTGGACTTGGTTTTAATGCCAGGACCAATGAGTACAAGGTCTTGCAAACATGCTGCAATGGCGAGTCTCAGATATACACCATTGGTACAAGAGTTTGGAGGAGCATTGGAAGGGCTCCTAGTGACAGTTTTATGTTACCGTTCAATTCGTTTCTGCATGGAGCTCTTCATTGGGTTCCCTACTCTTTTGATGGTTCTCAGTTTATTCAGTCTTTCAACTTCGAGACAGAACAATTTCGACCACTTCCCCCACCATTCAAAGGTCATTTGAAGTTATCCCAGCATTATAATTATCTAAAGTTGGGAGTGTTAAAAGGTTGTCTCTTCTTATGTGTGTTTGGTGCGTATAGTAGTGAATTTGACATGTGGGTTATGAAGGATTATGGTGTCCAAGAGTCTTGGACAAGAATTCTTGTCATTGAAAACTTGTATCCATGGATTCACTATGGCCTGCATAATGTATATGAACCCATTGTGTTCTTCAATGATGGGGAAATCCTAATGACATACAAGGATCGGGTGGTGGTTCGTTATAATCAAGAAACCAAGACTGTCTCACGAACTGGGATAATTCAAACTCAGTCTCAATTTCGTGCAATTGCATACTGCCCTTGCTTTGTTTCACTCCATGATGTTTCAAATGGAGAGGAGGTAATAAGGGTGAGAGACAATAACAAATTTGAAGAGATGGTTGCTAAAGGGAGCTATAATTGTGCTGGTTCCAGCAAGTCAGCTGATCATAAGTCGAAAAAACTGAAGTCAGGCTCTGGCCAACCTGCTCTGGGTGATGGTTCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

433

Amino Acids

49.27

Weight (kDa)

9.32

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 19 - 62 8.3e-09 F-box-like
F-box PF00646 19 - 58 5.3e-08 F-box domain
FBA_1 PF07734 115 - 364 1.8e-24 F-box associated beta propeller domain
FBA_3 PF08268 117 - 363 9.2e-18 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29150 FvH4_1g29150 FvH4_2g35201 FvH4_3g36371 FvH4_4g12590 FvH4_4g12590 FvH4_4g13903 FvH4_4g16630 FvH4_4g16630 FvH4_4g16640 FvH4_4g16640 FvH4_4g16640
malus_domestica MD03G1174100.v1.1 MD13G1177900.v1.1 MD13G1178000.v1.1 MD13G1178100.v1.1
prunus_persica Prupe.1G146800_v2.0.a1 Prupe.1G146900_v2.0.a1 Prupe.1G147000_v2.0.a1 Prupe.1G147100_v2.0.a1 Prupe.1G147200_v2.0.a1 Prupe.1G185600_v2.0.a1 Prupe.1G529300_v2.0.a1 Prupe.6G154200_v2.0.a1 Prupe.6G217100_v2.0.a1
pyrus_communis pycom03g13170 pycom03g13180 pycom05g29500 pycom13g15350 pycom13g15380 pycom13g15390 pycom13g15400
rosa_chinensis RchiOBHm_Chr4g0395601 RchiOBHm_Chr4g0395611 RchiOBHm_Chr4g0395621 RchiOBHm_Chr4g0413281 RchiOBHm_Chr4g0419621 RchiOBHm_Chr4g0419631 RchiOBHm_Chr4g0419901 RchiOBHm_Chr4g0419911 RchiOBHm_Chr4g0419981 RchiOBHm_Chr4g0420081 RchiOBHm_Chr4g0420091 RchiOBHm_Chr6g0306731
rosa_laevigata RLG00000006686 RLG00000007789 RLG00000007793 RLG00000007794 RLG00000007808 RLG00000007809 RLG00000011318
rosa_multiflora Rmu_co8471007.1_g000001 Rmu_sc0000717.1_g000001 Rmu_sc0003141.1_g000001 Rmu_sc0006133.1_g000013 Rmu_sc0013209.1_g000001 Rmu_sc0013209.1_g000002 Rmu_sc0017785.1_g000004 Rmu_sc0017786.1_g000003 Rmu_sc0023895.1_g000004 Rmu_sc0032897.1_g000004
rosa_roxburghii Rroxscaffold_5G00339000 Rroxscaffold_5G00357390 Rroxscaffold_5G00362460 Rroxscaffold_5G00362470 Rroxscaffold_5G00362730 Rroxscaffold_5G00362740 Rroxscaffold_5G00362800
rosa_rugosa Rorug04G0158700 Rorug04G0158800 Rorug04G0158800 Rorug04G0158900 Rorug04G0159000 Rorug04G0159100 Rorug04G0159200 Rorug04G0159300 Rorug04G0161200 Rorug04G0161300 Rorug04G0161300 Rorug04G0161400 Rorug04G0162000 Rorug04G0162100
rosa_samantha Rh1BG049900 Rh4AG179100 Rh4AG179200 Rh4AG220700 Rh4AG222300 Rh4AG222400 Rh4AG222700 Rh4AG222900 Rh4BG222400 Rh4BG222600 Rh4BG224700 Rh4BG224800 Rh4BG225300 Rh4BG225400 Rh4BG338700 Rh4BG338800 Rh4CG235500 Rh4DG174300 Rh4DG219400 Rh4DG219600 Rh4DG221300 Rh4DG221400 Rh4DG221700 Rh5CG465500 Rh5DG455700 Rh6BG417700 Rh6BG436500 Rh6BG436600 Rh6CG478500 Rh6CG478600 Rh6DG465600 Rh6DG465700 Rh6DG492000
rosa_wichuraiana Rw4G018980 Rw4G019160 Rw4G019170 Rw4G019190 Rw4G028680 Rw5G040160 Rw6G040460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 789, 1035
Acc36I ACCTGC 1 cut(s) 1285
AccB1I GGYRCC 1 cut(s) 413
AccII CGCG 1 cut(s) 292
AciI CCGC 1 cut(s) 339
AclWI GGATC 1 cut(s) 1023
AcoI YGGCCR 1 cut(s) 1270
AcsI RAATTY 5 cut(s) 364, 497, 857, 909, 1178
AcuI CTGAAG 2 cut(s) 369, 1277
AfaI GTAC 5 cut(s) 183, 289, 449, 536, 589
AfiI CCNNNNNNNGG 3 cut(s) 440, 761, 1283
AflIII ACRYGT 1 cut(s) 864
AjnI CCWGG 2 cut(s) 14, 521
AleI CACNNNNGTG 1 cut(s) 42
AluBI AGCT 4 cut(s) 74, 665, 1206, 1235
AluI AGCT 4 cut(s) 74, 665, 1206, 1235
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 6 cut(s) 573, 719, 827, 1060, 1089, 1161
AlwI GGATC 1 cut(s) 1023
AlwNI CAGNNNCTG 1 cut(s) 1268
AoxI GGCC 5 cut(s) 111, 398, 483, 949, 1270
ApeKI GCWGC 2 cut(s) 341, 555
ApoI RAATTY 5 cut(s) 364, 497, 857, 909, 1178
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
Asp700I GAANNNNTTC 1 cut(s) 735
AspS9I GGNCC 4 cut(s) 112, 219, 398, 525
AsuHPI GGTGA 3 cut(s) 189, 1174, 1298
AvaII GGWCC 2 cut(s) 219, 525
BaeI ACNNNNGTAYC 2 cut(s) 579, 612
BalI TGGCCA 1 cut(s) 1272
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 2 cut(s) 619, 667
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 328, 542
BccI CCATC 6 cut(s) 113, 404, 686, 983, 1183, 1283
BciT130I CCWGG 2 cut(s) 16, 523
BciVI GTATCC 1 cut(s) 942
BclI TGATCA 2 cut(s) 213, 1237
BcoDI GTCTC 6 cut(s) 573, 719, 827, 1060, 1089, 1161
BfaI CTAG 2 cut(s) 96, 621
BfuAI ACCTGC 1 cut(s) 1285
BfuI GTATCC 1 cut(s) 942
BisI GCNGC 2 cut(s) 342, 556
BlsI GCNGC 2 cut(s) 343, 557
Bme1390I CCNGG 2 cut(s) 16, 523
Bme18I GGWCC 2 cut(s) 219, 525
BmgT120I GGNCC 4 cut(s) 112, 219, 398, 525
BmiI GGNNCC 6 cut(s) 399, 415, 618, 678, 1222, 1294
BmrFI CCNGG 2 cut(s) 16, 523
BmrI ACTGGG 1 cut(s) 1074
BmuI ACTGGG 1 cut(s) 1074
BplI GAGNNNNNCTC 2 cut(s) 298, 330
Bpu10I CCTNAGC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 14, 935
BsaXI ACNNNNNCTCC 2 cut(s) 1137, 1167
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 761, 1283
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 4 cut(s) 149, 332, 494, 1069
Bse3DI GCAATG 2 cut(s) 379, 565
BseBI CCWGG 2 cut(s) 16, 523
BseDI CCNNGG 2 cut(s) 14, 935
BseLI CCNNNNNNNGG 3 cut(s) 440, 761, 1283
BseMI GCAATG 2 cut(s) 379, 565
BseMII CTCAG 3 cut(s) 584, 713, 1094
BseNI ACTGG 4 cut(s) 149, 332, 494, 1069
BseRI GAGGAG 2 cut(s) 616, 1163
BseXI GCAGC 2 cut(s) 328, 542
BseYI CCCAGC 1 cut(s) 780
BsgI GTGCAG 1 cut(s) 252
Bsh1236I CGCG 1 cut(s) 292
BshFI GGCC 5 cut(s) 113, 400, 485, 951, 1272
BshNI GGYRCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 1 cut(s) 279
BsiWI CGTACG 1 cut(s) 287
BslI CCNNNNNNNGG 3 cut(s) 440, 761, 1283
BsmAI GTCTC 6 cut(s) 573, 719, 827, 1060, 1089, 1161
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 5 cut(s) 113, 400, 485, 951, 1272
Bsp1286I GDGCHC 2 cut(s) 619, 667
Bsp1407I TGTACA 1 cut(s) 181
Bsp143I GATC 4 cut(s) 194, 213, 1015, 1237
Bsp19I CCATGG 1 cut(s) 935
BspACI CCGC 1 cut(s) 339
BspANI GGCC 5 cut(s) 113, 400, 485, 951, 1272
BspCNI CTCAG 3 cut(s) 583, 712, 1093
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 6 cut(s) 399, 415, 618, 678, 1222, 1294
BspMI ACCTGC 1 cut(s) 1285
BspPI GGATC 1 cut(s) 1023
BspQI GCTCTTC 1 cut(s) 672
BspT107I GGYRCC 1 cut(s) 413
BsrDI GCAATG 2 cut(s) 379, 565
BsrFI RCCGGY 1 cut(s) 278
BsrGI TGTACA 1 cut(s) 181
BsrI ACTGG 4 cut(s) 149, 332, 494, 1069
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 14, 935
BssMI GATC 4 cut(s) 194, 213, 1015, 1237
BssT1I CCWWGG 1 cut(s) 935
Bst2UI CCWGG 2 cut(s) 16, 523
Bst4CI ACNGT 4 cut(s) 188, 629, 642, 1054
Bst6I CTCTTC 3 cut(s) 672, 830, 1179
BstAUI TGTACA 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 280, 953
BstDEI CTNAG 5 cut(s) 251, 393, 570, 699, 1080
BstDSI CCRYGG 1 cut(s) 935
BstFNI CGCG 1 cut(s) 292
BstKTI GATC 4 cut(s) 197, 216, 1018, 1240
BstMAI GTCTC 6 cut(s) 573, 719, 827, 1060, 1089, 1161
BstMBI GATC 4 cut(s) 194, 213, 1015, 1237
BstMWI GCNNNNNNNGC 1 cut(s) 561
BstNI CCWGG 2 cut(s) 16, 523
BstNSI RCATGY 2 cut(s) 555, 868
BstSCI CCNGG 2 cut(s) 14, 521
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 2 cut(s) 328, 542
BsuI GTATCC 1 cut(s) 942
BsuRI GGCC 5 cut(s) 113, 400, 485, 951, 1272
BtgI CCRYGG 1 cut(s) 935
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 1 cut(s) 1285
Cac8I GCNNGC 2 cut(s) 280, 953
CaiI CAGNNNCTG 1 cut(s) 1268
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 4 cut(s) 112, 219, 398, 525
CseI GACGC 1 cut(s) 121
Csp6I GTAC 5 cut(s) 182, 288, 448, 535, 588
CviAII CATG 6 cut(s) 369, 552, 659, 865, 936, 1130
CviQI GTAC 5 cut(s) 182, 288, 448, 535, 588
DdeI CTNAG 5 cut(s) 251, 393, 570, 699, 1080
DpnI GATC 4 cut(s) 196, 215, 1017, 1239
DpnII GATC 4 cut(s) 194, 213, 1015, 1237
EaeI YGGCCR 1 cut(s) 1270
Eam1104I CTCTTC 3 cut(s) 672, 830, 1179
EarI CTCTTC 3 cut(s) 672, 830, 1179
Ecl136II GAGCTC 1 cut(s) 665
Eco130I CCWWGG 1 cut(s) 935
Eco24I GRGCYC 2 cut(s) 619, 667
Eco47I GGWCC 2 cut(s) 219, 525
Eco53kI GAGCTC 1 cut(s) 665
Eco57I CTGAAG 2 cut(s) 369, 1277
EcoICRI GAGCTC 1 cut(s) 665
EcoO109I RGGNCCY 2 cut(s) 112, 398
EcoRI GAATTC 1 cut(s) 909
EcoRII CCWGG 2 cut(s) 14, 521
EcoT14I CCWWGG 1 cut(s) 935
EcoT38I GRGCYC 2 cut(s) 619, 667
ErhI CCWWGG 1 cut(s) 935
FaeI CATG 6 cut(s) 372, 555, 662, 868, 939, 1133
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FatI CATG 6 cut(s) 368, 551, 658, 864, 935, 1129
FbaI TGATCA 2 cut(s) 213, 1237
Fnu4HI GCNGC 2 cut(s) 342, 556
FriOI GRGCYC 2 cut(s) 619, 667
Fsp4HI GCNGC 2 cut(s) 342, 556
FspBI CTAG 2 cut(s) 96, 621
GluI GCNGC 2 cut(s) 342, 556
GsaI CCCAGC 1 cut(s) 784
HaeIII GGCC 5 cut(s) 113, 400, 485, 951, 1272
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 121
Hin1II CATG 6 cut(s) 372, 555, 662, 868, 939, 1133
HindIII AAGCTT 1 cut(s) 72
HinfI GANTC 4 cut(s) 294, 566, 896, 940
HpaII CCGG 1 cut(s) 279
HphI GGTGA 3 cut(s) 189, 1174, 1298
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 2 cut(s) 349, 573
Hpy188III TCNNGA 6 cut(s) 217, 262, 467, 724, 1040, 1059
Hpy8I GTNNAC 1 cut(s) 184
HpyAV CCTTC 3 cut(s) 471, 606, 871
HpyCH4III ACNGT 4 cut(s) 188, 629, 642, 1054
HpyF10VI GCNNNNNNNGC 1 cut(s) 561
HpyF3I CTNAG 5 cut(s) 251, 393, 570, 699, 1080
Hsp92II CATG 6 cut(s) 372, 555, 662, 868, 939, 1133
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 2 cut(s) 213, 1237
Kzo9I GATC 4 cut(s) 194, 213, 1015, 1237
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 5 cut(s) 319, 603, 622, 662, 1203
Lsp1109I GCAGC 2 cut(s) 328, 542
MaeI CTAG 2 cut(s) 96, 621
MaeIII GTNAC 3 cut(s) 161, 623, 636
MalI GATC 4 cut(s) 196, 215, 1017, 1239
MboI GATC 4 cut(s) 194, 213, 1015, 1237
MboII GAAGA 4 cut(s) 659, 817, 973, 1196
MfeI CAATTG 2 cut(s) 121, 1098
MhlI GDGCHC 2 cut(s) 619, 667
MlsI TGGCCA 1 cut(s) 1272
MluNI TGGCCA 1 cut(s) 1272
MlyI GAGTC 2 cut(s) 575, 905
MmeI TCCRAC 1 cut(s) 484
MnlI CCTC 6 cut(s) 17, 177, 300, 594, 1141, 1144
Mox20I TGGCCA 1 cut(s) 1272
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 735
MscI TGGCCA 1 cut(s) 1272
MseI TTAA 2 cut(s) 516, 812
MslI CAYNNNNRTG 1 cut(s) 42
Msp20I TGGCCA 1 cut(s) 1272
MspA1I CMGCKG 2 cut(s) 341, 1235
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 2 cut(s) 16, 523
MunI CAATTG 2 cut(s) 121, 1098
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 2 cut(s) 16, 523
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 1 cut(s) 561
NaeI GCCGGC 1 cut(s) 280
NcoI CCATGG 1 cut(s) 935
NdeII GATC 4 cut(s) 194, 213, 1015, 1237
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 6 cut(s) 372, 555, 662, 868, 939, 1133
NlaIV GGNNCC 6 cut(s) 399, 415, 618, 678, 1222, 1294
NmuCI GTSAC 2 cut(s) 161, 623
NspI RCATGY 2 cut(s) 555, 868
OliI CACNNNNGTG 1 cut(s) 42
PciI ACATGT 1 cut(s) 864
PciSI GCTCTTC 1 cut(s) 672
PctI GAATGC 1 cut(s) 456
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 735
PfeI GAWTC 2 cut(s) 294, 940
Pfl23II CGTACG 1 cut(s) 287
PkrI GCNGC 2 cut(s) 343, 557
PleI GAGTC 2 cut(s) 574, 904
PpsI GAGTC 2 cut(s) 574, 904
PscI ACATGT 1 cut(s) 864
PsiI TTATAA 2 cut(s) 789, 1035
Psp124BI GAGCTC 1 cut(s) 667
Psp6I CCWGG 2 cut(s) 14, 521
PspFI CCCAGC 1 cut(s) 780
PspGI CCWGG 2 cut(s) 14, 521
PspLI CGTACG 1 cut(s) 287
PspN4I GGNNCC 6 cut(s) 399, 415, 618, 678, 1222, 1294
PspPI GGNCC 4 cut(s) 112, 219, 398, 525
PstNI CAGNNNCTG 1 cut(s) 1268
PvuII CAGCTG 1 cut(s) 1235
RsaI GTAC 5 cut(s) 183, 289, 449, 536, 589
RsaNI GTAC 5 cut(s) 182, 288, 448, 535, 588
RseI CAYNNNNRTG 1 cut(s) 42
SacI GAGCTC 1 cut(s) 667
SapI GCTCTTC 1 cut(s) 672
SaqAI TTAA 2 cut(s) 516, 812
SatI GCNGC 2 cut(s) 342, 556
Sau3AI GATC 4 cut(s) 194, 213, 1015, 1237
Sau96I GGNCC 4 cut(s) 112, 219, 398, 525
SchI GAGTC 2 cut(s) 575, 905
ScrFI CCNGG 2 cut(s) 16, 523
SduI GDGCHC 2 cut(s) 619, 667
SinI GGWCC 2 cut(s) 219, 525
SmiMI CAYNNNNRTG 1 cut(s) 42
SsiI CCGC 1 cut(s) 339
SspMI CTAG 2 cut(s) 96, 621
SstI GAGCTC 1 cut(s) 667
StyD4I CCNGG 2 cut(s) 14, 521
StyI CCWWGG 1 cut(s) 935
TaaI ACNGT 4 cut(s) 188, 629, 642, 1054
TaqI TCGA 4 cut(s) 297, 723, 739, 1247
TatI WGTACW 3 cut(s) 181, 447, 534
TfiI GAWTC 2 cut(s) 294, 940
Tru1I TTAA 2 cut(s) 516, 812
Tru9I TTAA 2 cut(s) 516, 812
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 2 cut(s) 161, 623
TseI GCWGC 2 cut(s) 341, 555
Tsp45I GTSAC 2 cut(s) 161, 623
TspDTI ATGAA 4 cut(s) 357, 659, 890, 981
TspRI CASTG 1 cut(s) 339
VpaK11BI GGWCC 2 cut(s) 219, 525
XapI RAATTY 5 cut(s) 364, 497, 857, 909, 1178
XceI RCATGY 2 cut(s) 555, 868
XmnI GAANNNNTTC 1 cut(s) 735
XspI CTAG 2 cut(s) 96, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.