Rh4AG222900

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000298
Physical Location & Seq
Forward (+)
52246300 .. 52251678
5379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG222900.1

Sequence Viewer

Length: 795 bp
ATGATATACACCATTGGTAGAGGAGTTTGGAGAAGTATTGGAAAGCATCCTAGTGGCTGTAGAGGAGGCCCAATACCATTCGATTCTTTTCTGCATGGAGCTCTTCATTGGATTCCCAGTTATTTCTATCCTCCTTCATCTATACAAGCCTTTGACTTTGAAAGAGAAGAGTTTCGACCACTACTACTTCCCCCACAAGTTTGCAAATATATTGGTTTGAGGTTAGGAGTCTTGAAACGTTGTCTCTTTCTATGTGTGTTTGGTGGTGATGATAGGAAATCTGACATGTGGCTTATGAAGAATTATGGTGTTCAAGAGTCTTGGACGAAAGTTCTTGTCATCGAACACTTGTATCCATGGATATACAGTGCCCCGAGTAATGTGTATGAGCCAATTATGTTCTTGAGTGATGTGGAAATCCTAATGACGTATGATAGATTTGTGGTCCATTATAATCTAGAAACAAAATGTCTACGAAAAACACGCATTGTTCAGACTCGGTCTCAGTTTCAAGCATTTGGTTACTCTCCTGGCTTTGTTTCACTCCATGATGTTGCAAATGGAGAGGAGCAAATGGAGTCTAGCAGAGCCCAGTTGACTGCCAGGCTGACTATGCCAGGAAACATGCACCTCAGGCCCAAGCCGCTAGAGCCCAAGCCGTTAACTATGCCCTCAGGCAACCTAGCCAAGCCAAAAGCTCAGGCCCAAGCCGCCACACCCCCAGAAGAGCACCAGCGGCCACGTCAGCAACAGTGGGGTCCAATTGTCTGGAAGGCCCGGATACTCCTTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

264

Amino Acids

30.39

Weight (kDa)

9.36

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 2 - 125 6.4e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29150 FvH4_1g29150 FvH4_2g35201 FvH4_3g36371 FvH4_4g12590 FvH4_4g12590 FvH4_4g13903 FvH4_4g16630 FvH4_4g16630 FvH4_4g16640 FvH4_4g16640 FvH4_4g16640
malus_domestica MD03G1174100.v1.1 MD13G1177900.v1.1 MD13G1178000.v1.1 MD13G1178100.v1.1
prunus_persica Prupe.1G146800_v2.0.a1 Prupe.1G146900_v2.0.a1 Prupe.1G147000_v2.0.a1 Prupe.1G147100_v2.0.a1 Prupe.1G147200_v2.0.a1 Prupe.1G185600_v2.0.a1 Prupe.1G529300_v2.0.a1 Prupe.6G154200_v2.0.a1 Prupe.6G217100_v2.0.a1
pyrus_communis pycom03g13170 pycom03g13180 pycom05g29500 pycom13g15350 pycom13g15380 pycom13g15390 pycom13g15400
rosa_chinensis RchiOBHm_Chr4g0395601 RchiOBHm_Chr4g0395611 RchiOBHm_Chr4g0395621 RchiOBHm_Chr4g0413281 RchiOBHm_Chr4g0419621 RchiOBHm_Chr4g0419631 RchiOBHm_Chr4g0419901 RchiOBHm_Chr4g0419911 RchiOBHm_Chr4g0419981 RchiOBHm_Chr4g0420081 RchiOBHm_Chr4g0420091 RchiOBHm_Chr6g0306731
rosa_laevigata RLG00000006686 RLG00000007789 RLG00000007793 RLG00000007794 RLG00000007808 RLG00000007809 RLG00000011318
rosa_multiflora Rmu_co8471007.1_g000001 Rmu_sc0000717.1_g000001 Rmu_sc0003141.1_g000001 Rmu_sc0006133.1_g000013 Rmu_sc0013209.1_g000001 Rmu_sc0013209.1_g000002 Rmu_sc0017785.1_g000004 Rmu_sc0017786.1_g000003 Rmu_sc0023895.1_g000004 Rmu_sc0032897.1_g000004
rosa_roxburghii Rroxscaffold_5G00339000 Rroxscaffold_5G00357390 Rroxscaffold_5G00362460 Rroxscaffold_5G00362470 Rroxscaffold_5G00362730 Rroxscaffold_5G00362740 Rroxscaffold_5G00362800
rosa_rugosa Rorug04G0158700 Rorug04G0158800 Rorug04G0158800 Rorug04G0158900 Rorug04G0159000 Rorug04G0159100 Rorug04G0159200 Rorug04G0159300 Rorug04G0161200 Rorug04G0161300 Rorug04G0161300 Rorug04G0161400 Rorug04G0162000 Rorug04G0162100
rosa_samantha Rh1BG049900 Rh4AG179100 Rh4AG179200 Rh4AG220700 Rh4AG222300 Rh4AG222400 Rh4AG222700 Rh4AG222900 Rh4BG222400 Rh4BG222600 Rh4BG224700 Rh4BG224800 Rh4BG225300 Rh4BG225400 Rh4BG338700 Rh4BG338800 Rh4CG235500 Rh4DG174300 Rh4DG219400 Rh4DG219600 Rh4DG221300 Rh4DG221400 Rh4DG221700 Rh5CG465500 Rh5DG455700 Rh6BG417700 Rh6BG436500 Rh6BG436600 Rh6CG478500 Rh6CG478600 Rh6DG465600 Rh6DG465700 Rh6DG492000
rosa_wichuraiana Rw4G018980 Rw4G019160 Rw4G019170 Rw4G019190 Rw4G028680 Rw5G040160 Rw6G040460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AccI GTMKAC 1 cut(s) 472
AciI CCGC 3 cut(s) 644, 711, 736
AclI AACGTT 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 737
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 4 cut(s) 161, 235, 314, 512
AjiI CACGTC 1 cut(s) 743
AjnI CCWGG 3 cut(s) 529, 602, 616
AluBI AGCT 2 cut(s) 101, 698
AluI AGCT 2 cut(s) 101, 698
Alw21I GWGCWC 2 cut(s) 103, 732
Alw26I GTCTC 2 cut(s) 248, 507
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 5 cut(s) 67, 635, 702, 737, 774
Asp700I GAANNNNTTC 1 cut(s) 171
AspS9I GGNCC 6 cut(s) 68, 445, 636, 703, 758, 775
AsuC2I CCSGG 1 cut(s) 778
AsuHPI GGTGA 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 2 cut(s) 445, 758
AxyI CCTNAGG 2 cut(s) 632, 673
BaeGI GKGCMC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 335, 368
BanII GRGCYC 3 cut(s) 103, 592, 654
Bbv12I GWGCWC 2 cut(s) 103, 732
BceAI ACGGC 1 cut(s) 643
BciT130I CCWGG 3 cut(s) 531, 604, 618
BciVI GTATCC 2 cut(s) 363, 774
BcnI CCSGG 1 cut(s) 778
BcoDI GTCTC 2 cut(s) 248, 507
BfaI CTAG 5 cut(s) 51, 458, 582, 647, 683
BfmI CTRYAG 1 cut(s) 58
BfuI GTATCC 2 cut(s) 363, 774
BisI GCNGC 3 cut(s) 644, 711, 737
BlsI GCNGC 3 cut(s) 645, 712, 738
Bme1390I CCNGG 4 cut(s) 531, 604, 618, 778
Bme18I GGWCC 2 cut(s) 445, 758
BmeT110I CYCGRG 1 cut(s) 373
BmgBI CACGTC 1 cut(s) 743
BmgT120I GGNCC 6 cut(s) 68, 445, 636, 703, 758, 775
BmiI GGNNCC 1 cut(s) 759
BmrFI CCNGG 4 cut(s) 531, 604, 618, 778
BmrI ACTGGG 2 cut(s) 111, 586
BmsI GCATC 1 cut(s) 55
BmuI ACTGGG 2 cut(s) 111, 586
Bpu10I CCTNAGC 1 cut(s) 699
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 778
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 117, 592
Bse21I CCTNAGG 2 cut(s) 632, 673
BseBI CCWGG 3 cut(s) 531, 604, 618
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 1 cut(s) 46
BseMII CTCAG 4 cut(s) 518, 646, 687, 713
BseNI ACTGG 2 cut(s) 117, 592
BseRI GAGGAG 3 cut(s) 36, 78, 581
BseSI GKGCMC 1 cut(s) 373
BshFI GGCC 5 cut(s) 69, 637, 704, 739, 776
BsiHKAI GWGCWC 2 cut(s) 103, 732
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 1 cut(s) 778
BsmAI GTCTC 2 cut(s) 248, 507
BsnI GGCC 5 cut(s) 69, 637, 704, 739, 776
Bso31I GGTCTC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 5 cut(s) 103, 373, 592, 654, 732
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 3 cut(s) 644, 711, 736
BspANI GGCC 5 cut(s) 69, 637, 704, 739, 776
BspCNI CTCAG 4 cut(s) 517, 645, 686, 712
BspLI GGNNCC 1 cut(s) 759
BspQI GCTCTTC 2 cut(s) 108, 720
BspTNI GGTCTC 1 cut(s) 507
BsrI ACTGG 2 cut(s) 117, 592
BssECI CCNNGG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 3 cut(s) 531, 604, 618
Bst4CI ACNGT 2 cut(s) 368, 753
Bst6I CTCTTC 3 cut(s) 108, 162, 720
BstDEI CTNAG 4 cut(s) 504, 632, 673, 699
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 46
BstMAI GTCTC 2 cut(s) 248, 507
BstMWI GCNNNNNNNGC 7 cut(s) 613, 634, 643, 649, 710, 736, 745
BstNI CCWGG 3 cut(s) 531, 604, 618
BstNSI RCATGY 2 cut(s) 289, 628
BstSCI CCNGG 4 cut(s) 529, 602, 616, 776
BstSFI CTRYAG 1 cut(s) 58
BstSLI GKGCMC 1 cut(s) 373
BstXI CCANNNNNNTGG 1 cut(s) 768
Bsu36I CCTNAGG 2 cut(s) 632, 673
BsuI GTATCC 2 cut(s) 363, 774
BsuRI GGCC 5 cut(s) 69, 637, 704, 739, 776
BtgI CCRYGG 1 cut(s) 356
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 1 cut(s) 46
BtsIMutI CAGTG 2 cut(s) 373, 758
Cfr13I GGNCC 6 cut(s) 68, 445, 636, 703, 758, 775
CviAII CATG 6 cut(s) 95, 286, 357, 548, 625, 792
DdeI CTNAG 4 cut(s) 504, 632, 673, 699
EaeI YGGCCR 1 cut(s) 737
Eam1104I CTCTTC 3 cut(s) 108, 162, 720
EarI CTCTTC 3 cut(s) 108, 162, 720
Ecl136II GAGCTC 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 356
Eco24I GRGCYC 3 cut(s) 103, 592, 654
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 445, 758
Eco53kI GAGCTC 1 cut(s) 101
Eco81I CCTNAGG 2 cut(s) 632, 673
Eco88I CYCGRG 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 101
EcoRII CCWGG 3 cut(s) 529, 602, 616
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 3 cut(s) 103, 592, 654
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 6 cut(s) 98, 289, 360, 551, 628, 795
FatI CATG 6 cut(s) 94, 285, 356, 547, 624, 791
FblI GTMKAC 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 644, 711, 737
FokI GGATG 1 cut(s) 33
FriOI GRGCYC 3 cut(s) 103, 592, 654
Fsp4HI GCNGC 3 cut(s) 644, 711, 737
FspBI CTAG 5 cut(s) 51, 458, 582, 647, 683
GluI GCNGC 3 cut(s) 644, 711, 737
HaeIII GGCC 5 cut(s) 69, 637, 704, 739, 776
HapII CCGG 1 cut(s) 778
Hin1II CATG 6 cut(s) 98, 289, 360, 551, 628, 795
HincII GTYRAC 2 cut(s) 597, 663
HindII GTYRAC 2 cut(s) 597, 663
HinfI GANTC 6 cut(s) 83, 112, 228, 317, 496, 578
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 1 cut(s) 778
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 3 cut(s) 473, 597, 663
Hpy188I TCNGA 2 cut(s) 283, 495
Hpy188III TCNNGA 5 cut(s) 232, 314, 403, 458, 769
Hpy8I GTNNAC 3 cut(s) 473, 597, 663
HpyAV CCTTC 2 cut(s) 144, 766
HpyCH4III ACNGT 2 cut(s) 368, 753
HpyCH4IV ACGT 3 cut(s) 238, 428, 742
HpyCH4V TGCA 4 cut(s) 94, 204, 557, 628
HpyF10VI GCNNNNNNNGC 7 cut(s) 613, 634, 643, 649, 710, 736, 745
HpyF3I CTNAG 4 cut(s) 504, 632, 673, 699
HpySE526I ACGT 3 cut(s) 238, 428, 742
Hsp92II CATG 6 cut(s) 98, 289, 360, 551, 628, 795
KspAI GTTAAC 1 cut(s) 663
LguI GCTCTTC 2 cut(s) 108, 720
LmnI GCTCC 2 cut(s) 98, 568
LweI GCATC 1 cut(s) 55
MaeI CTAG 5 cut(s) 51, 458, 582, 647, 683
MaeII ACGT 3 cut(s) 238, 428, 742
MaeIII GTNAC 1 cut(s) 521
MboII GAAGA 4 cut(s) 95, 179, 310, 737
MfeI CAATTG 1 cut(s) 762
MhlI GDGCHC 5 cut(s) 103, 373, 592, 654, 732
MluCI AATT 3 cut(s) 301, 393, 762
MlyI GAGTC 4 cut(s) 237, 326, 490, 587
MnlI CCTC 8 cut(s) 14, 56, 59, 141, 213, 559, 641, 682
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 1 cut(s) 662
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 736
MspI CCGG 1 cut(s) 778
MspR9I CCNGG 4 cut(s) 531, 604, 618, 778
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 3 cut(s) 531, 604, 618
MwoI GCNNNNNNNGC 7 cut(s) 613, 634, 643, 649, 710, 736, 745
NciI CCSGG 1 cut(s) 778
NcoI CCATGG 1 cut(s) 356
NlaIII CATG 6 cut(s) 98, 289, 360, 551, 628, 795
NlaIV GGNNCC 1 cut(s) 759
NspI RCATGY 2 cut(s) 289, 628
PciI ACATGT 1 cut(s) 285
PciSI GCTCTTC 2 cut(s) 108, 720
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 2 cut(s) 83, 112
PflFI GACNNNGTC 1 cut(s) 499
PkrI GCNGC 3 cut(s) 645, 712, 738
PleI GAGTC 4 cut(s) 236, 325, 490, 586
PpsI GAGTC 4 cut(s) 236, 325, 490, 586
PscI ACATGT 1 cut(s) 285
PsiI TTATAA 1 cut(s) 453
Psp124BI GAGCTC 1 cut(s) 103
Psp1406I AACGTT 1 cut(s) 238
Psp6I CCWGG 3 cut(s) 529, 602, 616
PspGI CCWGG 3 cut(s) 529, 602, 616
PspN4I GGNNCC 1 cut(s) 759
PspPI GGNCC 6 cut(s) 68, 445, 636, 703, 758, 775
PsyI GACNNNGTC 1 cut(s) 499
RseI CAYNNNNRTG 1 cut(s) 51
SacI GAGCTC 1 cut(s) 103
SapI GCTCTTC 2 cut(s) 108, 720
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 3 cut(s) 644, 711, 737
Sau96I GGNCC 6 cut(s) 68, 445, 636, 703, 758, 775
SchI GAGTC 4 cut(s) 237, 326, 490, 587
ScrFI CCNGG 4 cut(s) 531, 604, 618, 778
SduI GDGCHC 5 cut(s) 103, 373, 592, 654, 732
SetI ASST 8 cut(s) 103, 224, 241, 431, 633, 684, 700, 745
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 58
SinI GGWCC 2 cut(s) 445, 758
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 3 cut(s) 301, 393, 762
SsiI CCGC 3 cut(s) 644, 711, 736
SspMI CTAG 5 cut(s) 51, 458, 582, 647, 683
SstI GAGCTC 1 cut(s) 103
StyD4I CCNGG 4 cut(s) 529, 602, 616, 776
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 2 cut(s) 368, 753
TaiI ACGT 3 cut(s) 241, 431, 745
TaqI TCGA 3 cut(s) 81, 175, 342
TaqII GACCGA 1 cut(s) 489
TasI AATT 3 cut(s) 301, 393, 762
TauI GCSGC 3 cut(s) 646, 713, 739
TfiI GAWTC 2 cut(s) 83, 112
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 2 cut(s) 373, 758
TspDTI ATGAA 3 cut(s) 95, 126, 311
TspRI CASTG 2 cut(s) 373, 758
Tth111I GACNNNGTC 1 cut(s) 499
VpaK11BI GGWCC 2 cut(s) 445, 758
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 2 cut(s) 289, 628
XmiI GTMKAC 1 cut(s) 472
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 5 cut(s) 51, 458, 582, 647, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.