Rw4G028680

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000298
Physical Location & Seq
Reverse (-)
55309729 .. 55310795
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028680.1

Sequence Viewer

Length: 981 bp
ATGAGACGCAGGAACAGAAAGATCAACAGCGTTGAGCAACTAGAAGAAGAAGAAGAATGGCAACCCAAAGCTCCTTGCAGGTTTCATGAGCATCCGCAGGGTTTAGTCATGGACATTCTCTCCAGGCTCTCTCTTAAAACCCTCTTCAAATGCAGGTGTGTTTGCAAGTCCTGGCTTTTCATCACAGCGGACCCTCATTTTTCTCATCTCTGCCTTTCAAGAGTACCCATTGGTATCTTGATCAAGACCATTCCCATCAATAGGGATTCAAGGATTATTGATTTGACCCAAATTGAACAATCTACTGGATCTCACTTCCAGCTTGAGAAAGTGAGATTTAGTCCCAAATATAGCCTTCCCCCCCCATTTTCTGATTTTGATCTGATCAACTCCTGCAATGGCTTACTTCTTTTGGTCGGCATTGGAATAGATGTTTGCAATCCAGTTTCGGGTGAGTACATCACTATTCCGCTTCCTAAAGAAGCTTGCCTCAAAAATAGCTTTTTAGGACTTGGTTATAGCGCCTCAACCAATGAGTACAAAGTGTTGCAAAGTAGCTCATGCATAAACAAAGCTAAGGTCCTTGACGCTGAGTGTGAGGCTCTGATATACAGAATTGGTACCGGAGGAGGAGCTTGGAGAAGCATCGGAAAAACTCCTCCGGACGAAACCCGGGACCCTCCGTTTAGTTCTTTTCTACATGGAGCTCTTCACTGGATTTCCGCTGGTTCTCAAAATTTTTTTCGCACGCTACCCCCACCTGACTACTTTAAACCAATTCAGCAGAAATCTAAACAGTGTCTTAAATTGGAAGTGTTAGAAGGTTCTCTCTTTTTATGTGATTATGGTGTGGAAGAGTCTTGGACAAAAACTCTTGTTTTTGAAGGCTTGTATCCACCACCAGAACTCAACAATGATGCTTGTGAACCGCTGGTGTTTTTGAGGAATGGGGACATCCTATTGTCCTACAATGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

326

Amino Acids

36.83

Weight (kDa)

6.2

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 32 - 67 2.6e-07 F-box domain
F-box-like PF12937 32 - 72 7.8e-07 F-box-like
FBA_1 PF07734 125 - 242 1.6e-10 F-box associated beta propeller domain
FBA_3 PF08268 125 - 240 1.8e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000298)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29150 FvH4_1g29150 FvH4_2g35201 FvH4_3g36371 FvH4_4g12590 FvH4_4g12590 FvH4_4g13903 FvH4_4g16630 FvH4_4g16630 FvH4_4g16640 FvH4_4g16640 FvH4_4g16640
malus_domestica MD03G1174100.v1.1 MD13G1177900.v1.1 MD13G1178000.v1.1 MD13G1178100.v1.1
prunus_persica Prupe.1G146800_v2.0.a1 Prupe.1G146900_v2.0.a1 Prupe.1G147000_v2.0.a1 Prupe.1G147100_v2.0.a1 Prupe.1G147200_v2.0.a1 Prupe.1G185600_v2.0.a1 Prupe.1G529300_v2.0.a1 Prupe.6G154200_v2.0.a1 Prupe.6G217100_v2.0.a1
pyrus_communis pycom03g13170 pycom03g13180 pycom05g29500 pycom13g15350 pycom13g15380 pycom13g15390 pycom13g15400
rosa_chinensis RchiOBHm_Chr4g0395601 RchiOBHm_Chr4g0395611 RchiOBHm_Chr4g0395621 RchiOBHm_Chr4g0413281 RchiOBHm_Chr4g0419621 RchiOBHm_Chr4g0419631 RchiOBHm_Chr4g0419901 RchiOBHm_Chr4g0419911 RchiOBHm_Chr4g0419981 RchiOBHm_Chr4g0420081 RchiOBHm_Chr4g0420091 RchiOBHm_Chr6g0306731
rosa_laevigata RLG00000006686 RLG00000007789 RLG00000007793 RLG00000007794 RLG00000007808 RLG00000007809 RLG00000011318
rosa_multiflora Rmu_co8471007.1_g000001 Rmu_sc0000717.1_g000001 Rmu_sc0003141.1_g000001 Rmu_sc0006133.1_g000013 Rmu_sc0013209.1_g000001 Rmu_sc0013209.1_g000002 Rmu_sc0017785.1_g000004 Rmu_sc0017786.1_g000003 Rmu_sc0023895.1_g000004 Rmu_sc0032897.1_g000004
rosa_roxburghii Rroxscaffold_5G00339000 Rroxscaffold_5G00357390 Rroxscaffold_5G00362460 Rroxscaffold_5G00362470 Rroxscaffold_5G00362730 Rroxscaffold_5G00362740 Rroxscaffold_5G00362800
rosa_rugosa Rorug04G0158700 Rorug04G0158800 Rorug04G0158800 Rorug04G0158900 Rorug04G0159000 Rorug04G0159100 Rorug04G0159200 Rorug04G0159300 Rorug04G0161200 Rorug04G0161300 Rorug04G0161300 Rorug04G0161400 Rorug04G0162000 Rorug04G0162100
rosa_samantha Rh1BG049900 Rh4AG179100 Rh4AG179200 Rh4AG220700 Rh4AG222300 Rh4AG222400 Rh4AG222700 Rh4AG222900 Rh4BG222400 Rh4BG222600 Rh4BG224700 Rh4BG224800 Rh4BG225300 Rh4BG225400 Rh4BG338700 Rh4BG338800 Rh4CG235500 Rh4DG174300 Rh4DG219400 Rh4DG219600 Rh4DG221300 Rh4DG221400 Rh4DG221700 Rh5CG465500 Rh5DG455700 Rh6BG417700 Rh6BG436500 Rh6BG436600 Rh6CG478500 Rh6CG478600 Rh6DG465600 Rh6DG465700 Rh6DG492000
rosa_wichuraiana Rw4G018980 Rw4G019160 Rw4G019170 Rw4G019190 Rw4G028680 Rw5G040160 Rw6G040460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 144
Acc36I ACCTGC 2 cut(s) 69, 144
Acc65I GGTACC 1 cut(s) 620
AccB1I GGYRCC 1 cut(s) 620
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 5 cut(s) 95, 188, 470, 723, 929
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 1 cut(s) 736
AfaI GTAC 4 cut(s) 225, 458, 539, 622
AfiI CCNNNNNNNGG 2 cut(s) 261, 449
AgsI TTSAA 5 cut(s) 148, 219, 270, 296, 884
AjnI CCWGG 2 cut(s) 122, 170
AluBI AGCT 8 cut(s) 71, 322, 485, 501, 558, 575, 635, 707
AluI AGCT 8 cut(s) 71, 322, 485, 501, 558, 575, 635, 707
Alw21I GWGCWC 1 cut(s) 709
AlwI GGATC 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 672
Aor13HI TCCGGA 1 cut(s) 661
ApoI RAATTY 1 cut(s) 736
ArsI GACNNNNNNTTYG 2 cut(s) 564, 596
Asp718I GGTACC 1 cut(s) 620
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 3 cut(s) 190, 580, 676
AsuC2I CCSGG 2 cut(s) 673, 674
AsuHPI GGTGA 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 672
AvaII GGWCC 3 cut(s) 190, 580, 676
BanI GGYRCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 709
Bbv12I GWGCWC 1 cut(s) 709
BccI CCATC 1 cut(s) 263
BciT130I CCWGG 2 cut(s) 124, 172
BciVI GTATCC 1 cut(s) 903
BclI TGATCA 2 cut(s) 240, 384
BcnI CCSGG 2 cut(s) 673, 674
BfaI CTAG 1 cut(s) 41
BfoI RGCGCY 1 cut(s) 525
BfuAI ACCTGC 2 cut(s) 69, 144
BfuI GTATCC 1 cut(s) 903
Bme1390I CCNGG 4 cut(s) 124, 172, 673, 674
Bme18I GGWCC 3 cut(s) 190, 580, 676
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 3 cut(s) 190, 580, 676
BmiI GGNNCC 4 cut(s) 192, 622, 677, 678
BmrFI CCNGG 4 cut(s) 124, 172, 673, 674
BmsI GCATC 3 cut(s) 100, 654, 907
BpmI CTGGAG 1 cut(s) 106
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 2 cut(s) 673, 674
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 672
BsaWI WCCGGW 2 cut(s) 623, 661
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 261, 449
Bse1I ACTGG 3 cut(s) 310, 443, 719
Bse3DI GCAATG 1 cut(s) 403
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 1 cut(s) 661
BseBI CCWGG 2 cut(s) 124, 172
BseDI CCNNGG 1 cut(s) 672
BseGI GGATG 2 cut(s) 91, 954
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 2 cut(s) 261, 449
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 1 cut(s) 582
BseNI ACTGG 3 cut(s) 310, 443, 719
BseRI GAGGAG 3 cut(s) 642, 645, 648
BshNI GGYRCC 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 709
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 3 cut(s) 624, 662, 673
BslFI GGGAC 3 cut(s) 327, 689, 965
BslI CCNNNNNNNGG 2 cut(s) 261, 449
BsmFI GGGAC 3 cut(s) 327, 689, 965
BsoBI CYCGRG 1 cut(s) 672
Bsp1286I GDGCHC 1 cut(s) 709
Bsp13I TCCGGA 1 cut(s) 661
Bsp143I GATC 5 cut(s) 21, 240, 308, 379, 384
BspACI CCGC 5 cut(s) 95, 188, 470, 723, 929
BspCNI CTCAG 1 cut(s) 583
BspEI TCCGGA 1 cut(s) 661
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 4 cut(s) 192, 622, 677, 678
BspMI ACCTGC 2 cut(s) 69, 144
BspPI GGATC 1 cut(s) 316
BspQI GCTCTTC 1 cut(s) 714
BspT107I GGYRCC 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 403
BsrI ACTGG 3 cut(s) 310, 443, 719
BssECI CCNNGG 1 cut(s) 672
BssMI GATC 5 cut(s) 21, 240, 308, 379, 384
Bst2UI CCWGG 2 cut(s) 124, 172
Bst4CI ACNGT 1 cut(s) 798
Bst6I CTCTTC 3 cut(s) 149, 714, 849
BstC8I GCNNGC 2 cut(s) 487, 749
BstDEI CTNAG 2 cut(s) 576, 591
BstF5I GGATG 2 cut(s) 91, 954
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 5 cut(s) 24, 243, 311, 382, 387
BstMBI GATC 5 cut(s) 21, 240, 308, 379, 384
BstNI CCWGG 2 cut(s) 124, 172
BstSCI CCNGG 4 cut(s) 122, 170, 671, 672
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
BsuI GTATCC 1 cut(s) 903
BtsCI GGATG 2 cut(s) 91, 954
BtsIMutI CAGTG 2 cut(s) 712, 803
BveI ACCTGC 2 cut(s) 69, 144
Cac8I GCNNGC 2 cut(s) 487, 749
CciI TCATGA 1 cut(s) 85
CfoI GCGC 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 190, 580, 676
Cfr9I CCCGGG 1 cut(s) 672
CseI GACGC 2 cut(s) 15, 596
Csp6I GTAC 4 cut(s) 224, 457, 538, 621
CviAII CATG 4 cut(s) 86, 109, 561, 701
CviQI GTAC 4 cut(s) 224, 457, 538, 621
DdeI CTNAG 2 cut(s) 576, 591
DpnI GATC 5 cut(s) 23, 242, 310, 381, 386
DpnII GATC 5 cut(s) 21, 240, 308, 379, 384
DraI TTTAAA 1 cut(s) 772
Eam1104I CTCTTC 3 cut(s) 149, 714, 849
EarI CTCTTC 3 cut(s) 149, 714, 849
Ecl136II GAGCTC 1 cut(s) 707
Eco24I GRGCYC 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 190, 580, 676
Eco53kI GAGCTC 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 672
EcoICRI GAGCTC 1 cut(s) 707
EcoO109I RGGNCCY 2 cut(s) 580, 676
EcoRII CCWGG 2 cut(s) 122, 170
EcoT22I ATGCAT 1 cut(s) 566
EcoT38I GRGCYC 1 cut(s) 709
FaeI CATG 4 cut(s) 89, 112, 564, 704
FaqI GGGAC 3 cut(s) 327, 689, 965
FatI CATG 4 cut(s) 85, 108, 560, 700
FbaI TGATCA 2 cut(s) 240, 384
FokI GGATG 2 cut(s) 78, 941
FriOI GRGCYC 1 cut(s) 709
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 523
GsuI CTGGAG 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 525
HapII CCGG 3 cut(s) 624, 662, 673
HgaI GACGC 2 cut(s) 15, 596
HhaI GCGC 1 cut(s) 524
Hin1II CATG 4 cut(s) 89, 112, 564, 704
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 2 cut(s) 266, 857
HpaII CCGG 3 cut(s) 624, 662, 673
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 1 cut(s) 926
Hpy188I TCNGA 4 cut(s) 373, 384, 606, 650
Hpy188III TCNNGA 5 cut(s) 86, 219, 238, 244, 662
Hpy8I GTNNAC 1 cut(s) 926
HpyAV CCTTC 3 cut(s) 365, 815, 878
HpyCH4III ACNGT 1 cut(s) 798
HpyCH4V TGCA 7 cut(s) 78, 153, 165, 396, 438, 550, 564
HpyF3I CTNAG 2 cut(s) 576, 591
Hsp92II CATG 4 cut(s) 89, 112, 564, 704
HspAI GCGC 1 cut(s) 522
KflI GGGWCCC 1 cut(s) 676
Kpn2I TCCGGA 1 cut(s) 661
KpnI GGTACC 1 cut(s) 624
Ksp22I TGATCA 2 cut(s) 240, 384
Kzo9I GATC 5 cut(s) 21, 240, 308, 379, 384
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 3 cut(s) 76, 632, 704
LweI GCATC 3 cut(s) 100, 654, 907
MaeI CTAG 1 cut(s) 41
MalI GATC 5 cut(s) 23, 242, 310, 381, 386
MboI GATC 5 cut(s) 21, 240, 308, 379, 384
MboII GAAGA 7 cut(s) 56, 59, 62, 65, 136, 701, 866
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 1 cut(s) 709
MluCI AATT 5 cut(s) 291, 615, 736, 777, 806
MlyI GAGTC 1 cut(s) 866
Mph1103I ATGCAT 1 cut(s) 566
MroI TCCGGA 1 cut(s) 661
MseI TTAA 3 cut(s) 135, 771, 804
MspA1I CMGCKG 3 cut(s) 188, 725, 931
MspI CCGG 3 cut(s) 624, 662, 673
MspR9I CCNGG 4 cut(s) 124, 172, 673, 674
MvaI CCWGG 2 cut(s) 124, 172
NciI CCSGG 2 cut(s) 673, 674
NdeII GATC 5 cut(s) 21, 240, 308, 379, 384
NlaIII CATG 4 cut(s) 89, 112, 564, 704
NlaIV GGNNCC 4 cut(s) 192, 622, 677, 678
NsiI ATGCAT 1 cut(s) 566
PagI TCATGA 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 714
PfeI GAWTC 1 cut(s) 266
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
PpuMI RGGWCCY 2 cut(s) 580, 676
Psp124BI GAGCTC 1 cut(s) 709
Psp5II RGGWCCY 2 cut(s) 580, 676
Psp6I CCWGG 2 cut(s) 122, 170
PspGI CCWGG 2 cut(s) 122, 170
PspN4I GGNNCC 4 cut(s) 192, 622, 677, 678
PspPI GGNCC 3 cut(s) 190, 580, 676
PspPPI RGGWCCY 2 cut(s) 580, 676
PsuI RGATCY 1 cut(s) 308
RsaI GTAC 4 cut(s) 225, 458, 539, 622
RsaNI GTAC 4 cut(s) 224, 457, 538, 621
SacI GAGCTC 1 cut(s) 709
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 3 cut(s) 135, 771, 804
Sau3AI GATC 5 cut(s) 21, 240, 308, 379, 384
Sau96I GGNCC 3 cut(s) 190, 580, 676
SchI GAGTC 1 cut(s) 866
ScrFI CCNGG 4 cut(s) 124, 172, 673, 674
SduI GDGCHC 1 cut(s) 709
SfaNI GCATC 3 cut(s) 100, 654, 907
SinI GGWCC 3 cut(s) 190, 580, 676
SmaI CCCGGG 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 5 cut(s) 291, 615, 736, 777, 806
SsiI CCGC 5 cut(s) 95, 188, 470, 723, 929
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 709
StyD4I CCNGG 4 cut(s) 122, 170, 671, 672
TaaI ACNGT 1 cut(s) 798
TasI AATT 5 cut(s) 291, 615, 736, 777, 806
TatI WGTACW 2 cut(s) 456, 537
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 135, 771, 804
Tru9I TTAA 3 cut(s) 135, 771, 804
TscAI CASTG 2 cut(s) 719, 803
TspDTI ATGAA 2 cut(s) 74, 169
TspGWI ACGGA 1 cut(s) 672
TspMI CCCGGG 1 cut(s) 672
TspRI CASTG 2 cut(s) 719, 803
VpaK11BI GGWCC 3 cut(s) 190, 580, 676
XapI RAATTY 1 cut(s) 736
XmaI CCCGGG 1 cut(s) 672
XspI CTAG 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.