FvH4_3g03230

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000099
Physical Location & Seq
Reverse (-)
1717851 .. 1721952
4102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03230.t1

Sequence Viewer

Length: 2532 bp
ATGAATTCTAGTCAATGGTCTATGATCGCTGCATTGCTCATCATCTTCCTTCTTCATCTCCCCTCTTGCATTTCCACTGATTCCTTTACTCCAAACCAAACCATCAAAGACGGAGACGTTTTAGTCTCGAGCAGGAAGCTCTTTGCCCTCGGGTTCTTCAGCCCTGGTAATTCCGGCAAACGTTACGTTGGAGTTTGGTATAACAAAGTTCCAGAGCAGACCATTGTTTGGGTTGCAAATAGAAACAACCCCCTCGATGATACCTCTGGACTTTTAGTTATCGATGGAGATCATGGAGGCCTTGTCATATATGGAAAGGATAGAGAAGTCCCTCTTTGGTCTGCTAATGTTACTCTCTCTTCTCCACAGAATTCCATGGCCAAGCTCTTGGATACAGGAAACTTTGTTTTGTTTGATAATGGGAGCCAAAGGGTTTTGTGGGAAGGGTTTGATTATCCCTCTAATACACTGCTTCCATTTATGAAACTTGGGCTGAACCGGCGGTCCGGGTTGAACAGGTTCCTAACTTCTTGGAAGTCCCAGGATGACCCGGGAACTGGGAATTGTTCGTATAGGATTGATCCAGGTGGATTTCCGCAGCTGTTTTTGTACAAGGGTCGAGCTCCATTGTGGCGATCCGGGTCTTGGATCGGGCACAGATGGAGTGGTGTGCCTGAAATGACTCCGAATTTCCTCTTCAACGTTAGCTTTGTGAACAATCAAGACGAGTTATCTGTTGTGTATGGTATTACCAACGACTCAATCTTCTCAATGATGATGCTTGATGAATCAGGAACCGTCAAACGGTCAACGTGGCATGATCAGGTACACCAATGGATCAAGTTCTGGTCTGCTCCGGTAGAGGTATGTGACAACTATGGAAAGTGCGGTCCAAATAGCAACTGTGATCCTTACCATGCTGGTAAGTTCGAGTGTACATGCCTACCTGGGTTTGAACCTAAGTTGCACAATGAATGGTACTTGAGAGATGGCTCGGGTGGGTGTGTGAGGAGAAAAGGAGTATCCCTGTGCCAAAACGGAGAAGGGTTCGTGAAGGTTCCACTAGTGAAGGCACCAGACTCGTCTGCTGCGACTTTAAACTTGAACTTGGGTCTGAAAGAATGTGAAGATGAATGCTTAAGGAATTGTTCTTGCATGGCATACTCGAATGCAGATGACCTGCAAGGAGGACATGGGTGCGTGACATGGTATGGGGACTTGATGGATACAAGGACTTATACAAATGTCGGTCAAGATTTATATGTTCGAGTTGATTCAATTGAATTAGCTAAATACGCAAAGAAGTCGAATGGTTTTCCTAGCAAGAAGGGAAAGCTGGCAATTGTGATAACATCAATTTTGGTGTTCTTTCTTGTCATCTCTATCGTGTATTGGTACGTAAAGATGAAGAGAAAAGGTAAGGAAAGACGGGATTTATTTTCATATGGTGCCACACCTTCATCAATCTACTTCGAAAATTCTCCTTGTAGAACAGATCCTGATGATAGCAGATTAAAGCCAGATCTGCCTTTCTTTGATCTAAGCACCATATCCGCAGCCACGAAAAAATTCTCTGATTCGAACAAGCTTGGAGAAGGAGGTTTTGGCCCTGTTTATAAGGGTGTTCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTATGCAAAAATTCTGGCCAAGGAAACGAAGAGTTCAAGAATGAAGTTGTGCTAATTGCTAAACTTCAACACAGGAACCTTGTGAGAATCTTGGGTTATTGCGTTCAAGACGATGAGAAGATGCTAGTCTACGAATACCTGCCGAACAAAAGCCTGGACTCTTTCATTTTCAATGAAACGAAAAGGGCATTTCTGGATTGGCCAAGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGCATATTGTATCTTCATCAAGATTCAAGATTAAGAATCATCCATAGAGACCTAAAGGCCAGCAATGTTCTATTGGACTATGATATGAACCCAAAAATTGCAGATTTTGGTATGGCGAGAATATTCGGAGCAAACCAAATTGAAGCAAACACAAATCGCGTGGTCGGAACGTACGGTTACATGTCACCGGAATATACAATGGAAGGACTATTTTCAGTGAAGTCTGATGTATATAGTTTCGGTGTATTGCTGCTAGAAATCATTACTGGTAGAAAAAACACTGGTTACTACCACGATATTAATCCCGACTCAAATTTGGTTGGACATGTTTGGGACTTGTGGAAAGAAGGCCGAGCATTGGAAATCATCGACGCAACTTTGGGTGAATCCTACCCTGAAAGTGAAGTTCTCCGGTGCATTCATATCGCGCTCCTCTGCGTGCAAGAGTTTGCAAATGACCGGCCGACCATGTCAACAGTTGTGTCGGTGTTAGGCAACGATGCAGCTCTTCCTTCACCAAGAAAACCAGGATTTCTGCTGAAGAGAAGTCCCTATACCAGTGGAGACCCCAGCACAAGTACTGGAGATGTTAATTCCATAAACTATGTTACATGCACTACTGTTGAAGCTCGCTAA

Protein Analysis

844

Amino Acids

94.39

Weight (kDa)

6.91

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 179 7.3e-31 D-mannose binding lectin
S_locus_glycop PF00954 211 - 321 1.3e-29 S-locus glycoprotein domain
PAN_2 PF08276 344 - 410 1.5e-19 PAN-like domain
Pkinase PF00069 525 - 725 3.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 527 - 795 9.9e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1617
AasI GACNNNNNNGTC 1 cut(s) 122
Acc36I ACCTGC 2 cut(s) 1188, 1806
AccB1I GGYRCC 2 cut(s) 1072, 1448
AccB7I CCANNNNNTGG 1 cut(s) 228
AccI GTMKAC 1 cut(s) 1788
AccII CGCG 2 cut(s) 2055, 2324
AciI CCGC 4 cut(s) 502, 596, 888, 1554
AclI AACGTT 2 cut(s) 181, 702
AclWI GGATC 6 cut(s) 575, 630, 656, 845, 902, 1490
AcoI YGGCCR 4 cut(s) 378, 1675, 1859, 2357
AcsI RAATTY 7 cut(s) 4, 370, 688, 1477, 1568, 1669, 2209
AcuI CTGAAG 2 cut(s) 142, 2456
AfaI GTAC 7 cut(s) 611, 828, 937, 980, 1397, 2069, 2476
AfiI CCNNNNNNNGG 5 cut(s) 228, 557, 645, 804, 2254
AflII CTTAAG 1 cut(s) 1138
AflIII ACRYGT 2 cut(s) 2076, 2221
AhlI ACTAGT 1 cut(s) 1063
AjnI CCWGG 6 cut(s) 163, 540, 583, 946, 1812, 2422
AjuI GAANNNNNNNTTGG 2 cut(s) 1587, 1619
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 4 cut(s) 108, 130, 1938, 2454
AlwI GGATC 6 cut(s) 575, 630, 656, 845, 902, 1490
AlwNI CAGNNNCTG 1 cut(s) 1499
Ama87I CYCGRG 4 cut(s) 127, 149, 550, 994
AoxI GGCC 8 cut(s) 298, 378, 1606, 1675, 1859, 1953, 2245, 2357
ApeKI GCWGC 6 cut(s) 29, 598, 1088, 1556, 2146, 2399
ApoI RAATTY 7 cut(s) 4, 370, 688, 1477, 1568, 1669, 2209
AseI ATTAAT 1 cut(s) 2196
Asp700I GAANNNNTTC 2 cut(s) 518, 1568
AspLEI GCGC 1 cut(s) 2326
AspS9I GGNCC 3 cut(s) 504, 890, 1607
AsuC2I CCSGG 4 cut(s) 508, 551, 552, 640
AsuHPI GGTGA 3 cut(s) 2073, 2291, 2403
AsuII TTCGAA 2 cut(s) 1473, 1580
AvaI CYCGRG 4 cut(s) 127, 149, 550, 994
AvaII GGWCC 2 cut(s) 504, 890
BaeGI GKGCMC 1 cut(s) 657
BalI TGGCCA 3 cut(s) 380, 1677, 1861
BanI GGYRCC 2 cut(s) 1072, 1448
BanII GRGCYC 1 cut(s) 625
BarI GAAGNNNNNNTAC 2 cut(s) 2434, 2466
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 6 cut(s) 16, 610, 1075, 1568, 2133, 2411
BccI CCATC 5 cut(s) 110, 278, 654, 983, 1216
BceAI ACGGC 1 cut(s) 1634
BcgI CGANNNNNNTGC 6 cut(s) 1062, 1096, 1287, 1321, 2302, 2336
BciT130I CCWGG 6 cut(s) 165, 542, 585, 948, 1814, 2424
BciVI GTATCC 3 cut(s) 385, 1033, 1219
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 4 cut(s) 508, 551, 552, 640
BcoDI GTCTC 4 cut(s) 108, 130, 1938, 2454
BcuI ACTAGT 1 cut(s) 1063
BfaI CTAG 7 cut(s) 9, 1064, 1320, 1632, 1784, 1893, 2150
BfrI CTTAAG 1 cut(s) 1138
BfuAI ACCTGC 2 cut(s) 1188, 1806
BfuI GTATCC 3 cut(s) 385, 1033, 1219
BglII AGATCT 1 cut(s) 1522
BisI GCNGC 6 cut(s) 30, 599, 1089, 1557, 2147, 2400
BlsI GCNGC 6 cut(s) 31, 600, 1090, 1558, 2148, 2401
BmcAI AGTACT 1 cut(s) 2476
Bme18I GGWCC 2 cut(s) 504, 890
BmeT110I CYCGRG 4 cut(s) 127, 149, 550, 994
BmgT120I GGNCC 3 cut(s) 504, 890, 1607
BmiI GGNNCC 7 cut(s) 425, 521, 796, 1059, 1074, 1450, 1736
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 3 cut(s) 768, 1770, 2386
BmuI ACTGGG 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 2499
Bpu14I TTCGAA 2 cut(s) 1473, 1580
BpuEI CTTGAG 1 cut(s) 1003
BpuMI CCSGG 4 cut(s) 508, 551, 552, 640
Bsa29I ATCGAT 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 1399
BsaBI GATNNNNATC 2 cut(s) 288, 2196
BsaI GGTCTC 2 cut(s) 1938, 2454
BsaJI CCNNGG 7 cut(s) 148, 163, 375, 540, 550, 947, 1678
BsaWI WCCGGW 3 cut(s) 856, 2083, 2307
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 557, 645, 804, 2254
Bse118I RCCGGY 2 cut(s) 498, 2355
Bse1I ACTGG 5 cut(s) 562, 2167, 2182, 2454, 2482
Bse3DI GCAATG 3 cut(s) 32, 1887, 1966
Bse8I GATNNNNATC 2 cut(s) 288, 2196
BseBI CCWGG 6 cut(s) 165, 542, 585, 948, 1814, 2424
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 7 cut(s) 148, 163, 375, 540, 550, 947, 1678
BseGI GGATG 2 cut(s) 550, 1935
BseJI GATNNNNATC 2 cut(s) 288, 2196
BseLI CCNNNNNNNGG 5 cut(s) 228, 557, 645, 804, 2254
BseMI GCAATG 3 cut(s) 32, 1887, 1966
BseNI ACTGG 5 cut(s) 562, 2167, 2182, 2454, 2482
BseRI GAGGAG 2 cut(s) 1024, 2318
BseSI GKGCMC 1 cut(s) 657
BseX3I CGGCCG 1 cut(s) 2357
BseXI GCAGC 6 cut(s) 16, 610, 1075, 1568, 2133, 2411
BseYI CCCAGC 1 cut(s) 2465
Bsh1236I CGCG 2 cut(s) 2055, 2324
Bsh1285I CGRYCG 1 cut(s) 2360
BshFI GGCC 8 cut(s) 300, 380, 1608, 1677, 1861, 1955, 2247, 2359
BshNI GGYRCC 2 cut(s) 1072, 1448
BshVI ATCGAT 1 cut(s) 282
BsiEI CGRYCG 1 cut(s) 2360
BsiHKAI GWGCWC 1 cut(s) 625
BsiHKCI CYCGRG 4 cut(s) 127, 149, 550, 994
BsiSI CCGG 9 cut(s) 174, 499, 507, 551, 639, 857, 2084, 2308, 2356
BsiWI CGTACG 1 cut(s) 2067
BslFI GGGAC 5 cut(s) 314, 523, 1229, 2243, 2430
BslI CCNNNNNNNGG 5 cut(s) 228, 557, 645, 804, 2254
BsmAI GTCTC 4 cut(s) 108, 130, 1938, 2454
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 5 cut(s) 314, 523, 1229, 2243, 2430
BsmI GAATGC 3 cut(s) 1139, 1174, 2313
BsnI GGCC 8 cut(s) 300, 380, 1608, 1677, 1861, 1955, 2247, 2359
Bso31I GGTCTC 2 cut(s) 1938, 2454
BsoBI CYCGRG 4 cut(s) 127, 149, 550, 994
Bsp119I TTCGAA 2 cut(s) 1473, 1580
Bsp1286I GDGCHC 2 cut(s) 625, 657
Bsp1407I TGTACA 2 cut(s) 609, 935
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 502, 596, 888, 1554
BspANI GGCC 8 cut(s) 300, 380, 1608, 1677, 1861, 1955, 2247, 2359
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 2 cut(s) 2055, 2324
BspLI GGNNCC 7 cut(s) 425, 521, 796, 1059, 1074, 1450, 1736
BspMI ACCTGC 2 cut(s) 1188, 1806
BspPI GGATC 6 cut(s) 575, 630, 656, 845, 902, 1490
BspQI GCTCTTC 1 cut(s) 2409
BspT104I TTCGAA 2 cut(s) 1473, 1580
BspT107I GGYRCC 2 cut(s) 1072, 1448
BspTI CTTAAG 1 cut(s) 1138
BspTNI GGTCTC 2 cut(s) 1938, 2454
BsrDI GCAATG 3 cut(s) 32, 1887, 1966
BsrFI RCCGGY 2 cut(s) 498, 2355
BsrGI TGTACA 2 cut(s) 609, 935
BsrI ACTGG 5 cut(s) 562, 2167, 2182, 2454, 2482
BssAI RCCGGY 2 cut(s) 498, 2355
BssECI CCNNGG 7 cut(s) 148, 163, 375, 540, 550, 947, 1678
BssT1I CCWWGG 2 cut(s) 375, 1678
Bst2UI CCWGG 6 cut(s) 165, 542, 585, 948, 1814, 2424
Bst4CI ACNGT 6 cut(s) 799, 807, 905, 2072, 2374, 2518
Bst6I CTCTTC 6 cut(s) 364, 701, 1403, 1683, 2409, 2432
BstAFI CTTAAG 1 cut(s) 1138
BstAUI TGTACA 2 cut(s) 609, 935
BstBAI YACGTR 1 cut(s) 1399
BstBI TTCGAA 2 cut(s) 1473, 1580
BstC8I GCNNGC 4 cut(s) 1338, 1957, 2336, 2527
BstDEI CTNAG 2 cut(s) 960, 1541
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 550, 1935
BstFNI CGCG 2 cut(s) 2055, 2324
BstHHI GCGC 1 cut(s) 2326
BstMAI GTCTC 4 cut(s) 108, 130, 1938, 2454
BstMCI CGRYCG 1 cut(s) 2360
BstMWI GCNNNNNNNGC 3 cut(s) 499, 1295, 1525
BstNI CCWGG 6 cut(s) 165, 542, 585, 948, 1814, 2424
BstNSI RCATGY 4 cut(s) 942, 2080, 2225, 2511
BstSLI GKGCMC 1 cut(s) 657
BstSNI TACGTA 1 cut(s) 1399
BstUI CGCG 2 cut(s) 2055, 2324
BstV1I GCAGC 6 cut(s) 16, 610, 1075, 1568, 2133, 2411
BstX2I RGATCY 2 cut(s) 1495, 1522
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 2 cut(s) 1495, 1522
BstZI CGGCCG 1 cut(s) 2357
Bsu15I ATCGAT 1 cut(s) 282
BsuI GTATCC 3 cut(s) 385, 1033, 1219
BsuRI GGCC 8 cut(s) 300, 380, 1608, 1677, 1861, 1955, 2247, 2359
BsuTUI ATCGAT 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 550, 1935
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 5 cut(s) 75, 467, 2118, 2175, 2461
BveI ACCTGC 2 cut(s) 1188, 1806
Cac8I GCNNGC 4 cut(s) 1338, 1957, 2336, 2527
CaiI CAGNNNCTG 1 cut(s) 1499
CfoI GCGC 1 cut(s) 2326
Cfr10I RCCGGY 2 cut(s) 498, 2355
Cfr13I GGNCC 3 cut(s) 504, 890, 1607
Cfr9I CCCGGG 1 cut(s) 550
ClaI ATCGAT 1 cut(s) 282
CpoI CGGWCCG 1 cut(s) 504
CseI GACGC 2 cut(s) 1875, 2276
Csp6I GTAC 7 cut(s) 610, 827, 936, 979, 1396, 2068, 2475
CspCI CAANNNNNGTGG 2 cut(s) 2037, 2072
CspI CGGWCCG 1 cut(s) 504
CviQI GTAC 7 cut(s) 610, 827, 936, 979, 1396, 2068, 2475
DdeI CTNAG 2 cut(s) 960, 1541
DraI TTTAAA 1 cut(s) 1098
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 4 cut(s) 378, 1675, 1859, 2357
EagI CGGCCG 1 cut(s) 2357
Eam1104I CTCTTC 6 cut(s) 364, 701, 1403, 1683, 2409, 2432
EarI CTCTTC 6 cut(s) 364, 701, 1403, 1683, 2409, 2432
Ecl136II GAGCTC 1 cut(s) 623
EclXI CGGCCG 1 cut(s) 2357
Eco105I TACGTA 1 cut(s) 1399
Eco130I CCWWGG 2 cut(s) 375, 1678
Eco147I AGGCCT 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 2 cut(s) 1938, 2454
Eco47I GGWCC 2 cut(s) 504, 890
Eco52I CGGCCG 1 cut(s) 2357
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 2 cut(s) 142, 2456
Eco88I CYCGRG 4 cut(s) 127, 149, 550, 994
EcoICRI GAGCTC 1 cut(s) 623
EcoRI GAATTC 2 cut(s) 4, 370
EcoRII CCWGG 6 cut(s) 163, 540, 583, 946, 1812, 2422
EcoT14I CCWWGG 2 cut(s) 375, 1678
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 2 cut(s) 375, 1678
Esp3I CGTCTC 1 cut(s) 108
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 5 cut(s) 314, 523, 1229, 2243, 2430
FauNDI CATATG 1 cut(s) 1444
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 1 cut(s) 1788
Fnu4HI GCNGC 6 cut(s) 30, 599, 1089, 1557, 2147, 2400
FokI GGATG 2 cut(s) 557, 1922
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 6 cut(s) 30, 599, 1089, 1557, 2147, 2400
FspBI CTAG 7 cut(s) 9, 1064, 1320, 1632, 1784, 1893, 2150
GlaI GCGC 1 cut(s) 2325
GluI GCNGC 6 cut(s) 30, 599, 1089, 1557, 2147, 2400
GsaI CCCAGC 1 cut(s) 2469
GsuI CTGGAG 1 cut(s) 2499
HaeIII GGCC 8 cut(s) 300, 380, 1608, 1677, 1861, 1955, 2247, 2359
HapII CCGG 9 cut(s) 174, 499, 507, 551, 639, 857, 2084, 2308, 2356
HgaI GACGC 2 cut(s) 1875, 2276
HhaI GCGC 1 cut(s) 2326
Hin6I GCGC 1 cut(s) 2324
HinP1I GCGC 1 cut(s) 2324
HincII GTYRAC 2 cut(s) 810, 2370
HindII GTYRAC 2 cut(s) 810, 2370
HindIII AAGCTT 1 cut(s) 1586
HpaII CCGG 9 cut(s) 174, 499, 507, 551, 639, 857, 2084, 2308, 2356
HphI GGTGA 3 cut(s) 2073, 2291, 2403
Hpy166II GTNNAC 6 cut(s) 715, 810, 829, 936, 1789, 2370
Hpy188I TCNGA 6 cut(s) 687, 1116, 1576, 2024, 2063, 2122
Hpy8I GTNNAC 6 cut(s) 715, 810, 829, 936, 1789, 2370
Hpy99I CGWCG 1 cut(s) 2270
HpyCH4III ACNGT 6 cut(s) 799, 807, 905, 2072, 2374, 2518
HpyCH4IV ACGT 7 cut(s) 117, 181, 186, 702, 812, 1398, 2066
HpyF10VI GCNNNNNNNGC 3 cut(s) 499, 1295, 1525
HpyF3I CTNAG 2 cut(s) 960, 1541
HpySE526I ACGT 7 cut(s) 117, 181, 186, 702, 812, 1398, 2066
HspAI GCGC 1 cut(s) 2324
Ksp22I TGATCA 1 cut(s) 820
LguI GCTCTTC 1 cut(s) 2409
LmnI GCTCC 5 cut(s) 423, 628, 859, 2024, 2331
Lsp1109I GCAGC 6 cut(s) 16, 610, 1075, 1568, 2133, 2411
LweI GCATC 3 cut(s) 768, 1770, 2386
MaeI CTAG 7 cut(s) 9, 1064, 1320, 1632, 1784, 1893, 2150
MaeII ACGT 7 cut(s) 117, 181, 186, 702, 812, 1398, 2066
MaeIII GTNAC 8 cut(s) 182, 349, 869, 1201, 2072, 2079, 2180, 2503
MfeI CAATTG 2 cut(s) 1278, 1341
MflI RGATCY 2 cut(s) 1495, 1522
MhlI GDGCHC 2 cut(s) 625, 657
MlsI TGGCCA 3 cut(s) 380, 1677, 1861
MluNI TGGCCA 3 cut(s) 380, 1677, 1861
MlyI GAGTC 5 cut(s) 676, 752, 1073, 1811, 2198
MmeI TCCRAC 3 cut(s) 169, 2041, 2197
Mox20I TGGCCA 3 cut(s) 380, 1677, 1861
MroXI GAANNNNTTC 2 cut(s) 518, 1568
MscI TGGCCA 3 cut(s) 380, 1677, 1861
MseI TTAA 6 cut(s) 1097, 1139, 1514, 1928, 2196, 2487
Msp20I TGGCCA 3 cut(s) 380, 1677, 1861
MspA1I CMGCKG 1 cut(s) 601
MspCI CTTAAG 1 cut(s) 1138
MspI CCGG 9 cut(s) 174, 499, 507, 551, 639, 857, 2084, 2308, 2356
MunI CAATTG 2 cut(s) 1278, 1341
Mva1269I GAATGC 3 cut(s) 1139, 1174, 2313
MvaI CCWGG 6 cut(s) 165, 542, 585, 948, 1814, 2424
MvnI CGCG 2 cut(s) 2055, 2324
MwoI GCNNNNNNNGC 3 cut(s) 499, 1295, 1525
NciI CCSGG 4 cut(s) 508, 551, 552, 640
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 1444
NlaIV GGNNCC 7 cut(s) 425, 521, 796, 1059, 1074, 1450, 1736
NmeAIII GCCGAG 1 cut(s) 2273
NmuCI GTSAC 3 cut(s) 869, 1201, 2079
NspI RCATGY 4 cut(s) 942, 2080, 2225, 2511
NspV TTCGAA 2 cut(s) 1473, 1580
PaeR7I CTCGAG 1 cut(s) 127
PceI AGGCCT 1 cut(s) 300
PciI ACATGT 2 cut(s) 2076, 2221
PciSI GCTCTTC 1 cut(s) 2409
PcsI WCGNNNNNNNCGW 1 cut(s) 1088
PctI GAATGC 3 cut(s) 1139, 1174, 2313
PdmI GAANNNNTTC 2 cut(s) 518, 1568
PfeI GAWTC 8 cut(s) 80, 788, 1274, 1577, 1746, 1919, 1932, 2282
Pfl23II CGTACG 1 cut(s) 2067
PflFI GACNNNGTC 1 cut(s) 2365
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 6 cut(s) 31, 600, 1090, 1558, 2148, 2401
PleI GAGTC 5 cut(s) 676, 752, 1073, 1811, 2198
PpsI GAGTC 5 cut(s) 676, 752, 1073, 1811, 2198
Ppu21I YACGTR 1 cut(s) 1399
PscI ACATGT 2 cut(s) 2076, 2221
PshBI ATTAAT 1 cut(s) 2196
PsiI TTATAA 1 cut(s) 1617
Psp124BI GAGCTC 1 cut(s) 625
Psp1406I AACGTT 2 cut(s) 181, 702
Psp6I CCWGG 6 cut(s) 163, 540, 583, 946, 1812, 2422
PspFI CCCAGC 1 cut(s) 2465
PspGI CCWGG 6 cut(s) 163, 540, 583, 946, 1812, 2422
PspLI CGTACG 1 cut(s) 2067
PspN4I GGNNCC 7 cut(s) 425, 521, 796, 1059, 1074, 1450, 1736
PspPI GGNCC 3 cut(s) 504, 890, 1607
PstNI CAGNNNCTG 1 cut(s) 1499
PsuI RGATCY 2 cut(s) 1495, 1522
PsyI GACNNNGTC 1 cut(s) 2365
PvuII CAGCTG 1 cut(s) 601
RsaI GTAC 7 cut(s) 611, 828, 937, 980, 1397, 2069, 2476
RsaNI GTAC 7 cut(s) 610, 827, 936, 979, 1396, 2068, 2475
Rsr2I CGGWCCG 1 cut(s) 504
RsrII CGGWCCG 1 cut(s) 504
SacI GAGCTC 1 cut(s) 625
SapI GCTCTTC 1 cut(s) 2409
SaqAI TTAA 6 cut(s) 1097, 1139, 1514, 1928, 2196, 2487
SatI GCNGC 6 cut(s) 30, 599, 1089, 1557, 2147, 2400
Sau96I GGNCC 3 cut(s) 504, 890, 1607
ScaI AGTACT 1 cut(s) 2476
SchI GAGTC 5 cut(s) 676, 752, 1073, 1811, 2198
SduI GDGCHC 2 cut(s) 625, 657
SfaNI GCATC 3 cut(s) 768, 1770, 2386
Sfr274I CTCGAG 1 cut(s) 127
SfuI TTCGAA 2 cut(s) 1473, 1580
SinI GGWCC 2 cut(s) 504, 890
SlaI CTCGAG 1 cut(s) 127
SmaI CCCGGG 1 cut(s) 552
SmlI CTYRAG 3 cut(s) 127, 982, 1138
SmoI CTYRAG 3 cut(s) 127, 982, 1138
SnaBI TACGTA 1 cut(s) 1399
SpeI ACTAGT 1 cut(s) 1063
SseBI AGGCCT 1 cut(s) 300
SsiI CCGC 4 cut(s) 502, 596, 888, 1554
SspI AATATT 1 cut(s) 2019
SspMI CTAG 7 cut(s) 9, 1064, 1320, 1632, 1784, 1893, 2150
SstI GAGCTC 1 cut(s) 625
StuI AGGCCT 1 cut(s) 300
StyI CCWWGG 2 cut(s) 375, 1678
TaaI ACNGT 6 cut(s) 799, 807, 905, 2072, 2374, 2518
TaiI ACGT 7 cut(s) 120, 184, 189, 705, 815, 1401, 2069
TaqII GACCGA 1 cut(s) 1238
TatI WGTACW 3 cut(s) 609, 935, 2474
TfiI GAWTC 8 cut(s) 80, 788, 1274, 1577, 1746, 1919, 1932, 2282
Tru1I TTAA 6 cut(s) 1097, 1139, 1514, 1928, 2196, 2487
Tru9I TTAA 6 cut(s) 1097, 1139, 1514, 1928, 2196, 2487
TscAI CASTG 5 cut(s) 82, 474, 2118, 2182, 2461
TseFI GTSAC 3 cut(s) 869, 1201, 2079
TseI GCWGC 6 cut(s) 29, 598, 1088, 1556, 2146, 2399
Tsp45I GTSAC 3 cut(s) 869, 1201, 2079
TspGWI ACGGA 2 cut(s) 126, 1053
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 5 cut(s) 82, 474, 2118, 2182, 2461
Tth111I GACNNNGTC 1 cut(s) 2365
Van91I CCANNNNNTGG 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 1138
VpaK11BI GGWCC 2 cut(s) 504, 890
VspI ATTAAT 1 cut(s) 2196
XapI RAATTY 7 cut(s) 4, 370, 688, 1477, 1568, 1669, 2209
XceI RCATGY 4 cut(s) 942, 2080, 2225, 2511
XhoI CTCGAG 1 cut(s) 127
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 1 cut(s) 1788
XmnI GAANNNNTTC 2 cut(s) 518, 1568
XspI CTAG 7 cut(s) 9, 1064, 1320, 1632, 1784, 1893, 2150
ZrmI AGTACT 1 cut(s) 2476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.