MD17G1273200.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000099
Physical Location & Seq
Forward (+)
33526361 .. 33530129
3769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1273200.v1.1.491

Sequence Viewer

Length: 2487 bp
ATGAATTCTACTAAATTCTTTTTTGTCATAATTATATTGCTTATCTTCCTTGTGATTCTTCCCTCTTCCCTTTCGATTGCCCTAGATGCCATTACACCAAACCAACCCCTAAGAGATGGCGACGTTCTTCTATCTACCACTAAAATCTTTGCACTAGGCTTTTTTAGCCCAGGCAATTCTCATAATCGTTATCTTGGAGTTTGGTACAACAAAATTCCAATCCAAACCATCGTCTGGACTGCAAACAGAGACAACCCCGTAATTCCTACTGCTGGAGCTGGGCTCTTAGCTATTCATGGAAATCATGGAGGCCTTGTTATTTATTGGAAGGACCAAAATACCCCTCTTTGGTCTGCTAATGTCACGGTCTCTTCGCCAAACAATTCCGTAATAGCCAAACTTTGGGATACAGGAAATCTTGTATTGCTTGAGAACAATGGTAGTAACGCAAGGGTGCTGTGGCAAGGCTTTGATTACGCCACAGATACAGTGCTTCCATCGATGAAGATTGGGCTGAACCGGCGGTCAGGGCTGAAGTGGCTCCTAACATCTTGGAAGTCCCAAGATGATCCCGGATCGGGGAATTTTTCGTATGAGATTGACCCAGCTGAGTTTCCGCAGTTCTTTTTTTACAAGGGTCGTCGAACCGAATGGTTTCGAGTCGGAACTTGGACCGGGCAGAGATTGAGTGGGGTCCCCGGATACGTACCTAATAGTTTCCGAAGCTTTGTGAACGATAAAGATGAGATATATACAATGCTTCATGTCACAGATAGCTCAGTATTCGTAAGAGCGGTGCTAGATGAATCAGGAATTCTTAAACGGTTCGTATGGCGAGATCATGAAAACAAATGGATCGAAGTAAGCTGTAGCCCTGGTGAGTGGTGTGACTACTATGGACAGTGTGGTCCAAATGGCATCTGTGACCCATACAGAAATTATAACTTTTCTTGCCTTTGTCTGCCCGGATTTGAACCCAAGTCCCCCCAGGATTGGTATCTGAAAGATGGATTGGGTGGATGCCTGAGAAAATCGGGAGCGTCTACATGCCAAAAAGGGGAAGGGTTTGTTAAGGTGGCACGAGTGAAGGTACCCGACTCATCTGGGGCACGTGTGAATATGAGTCTGAGTCTGCAAGGGTGTGAGCAAGAGTGCATGAAGAATTGTTCTTGCATGGCATACGCGAGTGCAGATGAAAGGGGAGGAGGGGAAGGGTTGCGTGACGTGGTACGGGGACTTGATAGACACAAGAACTTATTCAAATGTTGGGCAGGACTTGTATCTCAATTTGCAAATGGTTCTGTAATTAGCAAGAAAGCAAGGCTGGCAATTTCATTATTATCCGCTCTTGTGTTCCTTGGCCTAGTATTCCTTTTGTGTTGGTTGGTAAGGAGGAAGAGCAAAGCATGGAGGCAGAGAAAATATATGTTTTTCATGGAAGATAGAACAGATCTCGATGATCAAAGTAAAATAAACTCAGAATTACAATTCTTTGATCTAACAACCGTCGCAGCCGCCACGGACAATTTCTCTGTAGCGAACAAGCTTGGGAAAGGAGGTTTTGGCACAGTCTATAAGGGTGTACTTCATAATGGAAAGGAAGTAGCGGTGAAACGACTATCGAAGAATTCTGGTCAAGGAATTGAAGAGTTTAAGAATGAAGTTATGATAATTGCAAAACTCCAACACAGGAACCTTGTCAAGATTATAGGTTGCTGCATTGAAGATGAAGAGAAGATGCTAATCTATGAATACGCACCAAACAAAAGTTTGGACTCTTTTATTTTTGATGAAACAAAAAGAAATCTTTTAGATTGGACAAAACGCTTTGAGATTATCTGTGGGATTGCTAGAGGGATTTTATATCTTCATCAAGATTCGAGATTAAGGATTATCCATAGAGATCTAAAGGCCAGTAATATTCTATTGGATGCGTCTATGAGCCCCAAAATAGCAGATTTTGGTATGGCAAGAATATTTCTTGGGGACCAATGTGAAGCAAATACATATCGTGTGGTCGGAACATATGGTTATATGTCACCAGAGTATGCAATGGAAGGAATTTTTTCAGTAAAATCTGATGTGTACAGTTTTGGCGTATTACTGCTGGAAATCATAACTGGCAGAAGGAATTCCGGTCATCACCACAAAAAATATCCCCACTCAAATTTGGTTGGATATGTTTGGGACTTGTGGAGAGAAGGCAATGCCTTGGAAATCGTTGATCCATCCATGGGCGAATCGTACCATGTCAACGAAGTTGTGAGATGCATCCAAATTGCCCTCTTGTGTGTGCAAGAGTTTGCTGCTGACCGGCCAACCATGTCGGCGGTTGTTTTCATGCTAGGTAACGAGGCAGCAGTTCCTTCCCCAACACAGCCTGCATTTTTGCTGGGGAGGAATCGTACTAGAGTAGATCCATCAACGAGTACTGAAGGAGCTAGTTCTGTAAATGATGTGACATGTACAGAAATAGAAGTTCGCTAA

Protein Analysis

829

Amino Acids

92.82

Weight (kDa)

8.41

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 186 2.2e-28 D-mannose binding lectin
S_locus_glycop PF00954 219 - 328 1.4e-21 S-locus glycoprotein domain
PAN_2 PF08276 350 - 403 5.3e-14 PAN-like domain
Pkinase PF00069 511 - 721 2.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 512 - 779 3.5e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 942
AasI GACNNNNNNGTC 1 cut(s) 906
Acc65I GGTACC 1 cut(s) 1090
AccB1I GGYRCC 1 cut(s) 1090
AccB7I CCANNNNNTGG 2 cut(s) 234, 402
AccBSI CCGCTC 2 cut(s) 794, 1346
AccI GTMKAC 1 cut(s) 1043
AccII CGCG 1 cut(s) 1184
AciI CCGC 7 cut(s) 523, 617, 794, 1344, 1515, 1607, 2330
AclWI GGATC 5 cut(s) 563, 583, 863, 2219, 2411
AcoI YGGCCR 1 cut(s) 2315
AcsI RAATTY 9 cut(s) 4, 14, 213, 583, 813, 1627, 2061, 2131, 2167
AcuI CTGAAG 2 cut(s) 554, 2454
AcvI CACGTG 1 cut(s) 1112
AfiI CCNNNNNNNGG 9 cut(s) 234, 272, 348, 402, 578, 579, 993, 1057, 2234
AflIII ACRYGT 2 cut(s) 1111, 2462
AgsI TTSAA 4 cut(s) 974, 1261, 1646, 1724
AjiI CACGTC 1 cut(s) 1225
AjnI CCWGG 3 cut(s) 169, 874, 987
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
AluBI AGCT 8 cut(s) 278, 290, 608, 726, 777, 867, 1546, 2441
AluI AGCT 8 cut(s) 278, 290, 608, 726, 777, 867, 1546, 2441
Alw26I GTCTC 2 cut(s) 243, 373
AlwI GGATC 5 cut(s) 563, 583, 863, 2219, 2411
AoxI GGCC 4 cut(s) 310, 1360, 1911, 2315
ApeKI GCWGC 4 cut(s) 1511, 1716, 2306, 2357
ApoI RAATTY 9 cut(s) 4, 14, 213, 583, 813, 1627, 2061, 2131, 2167
Asp700I GAANNNNTTC 4 cut(s) 654, 1256, 2065, 2131
Asp718I GGTACC 1 cut(s) 1090
AspS9I GGNCC 5 cut(s) 331, 672, 694, 908, 1987
AsuC2I CCSGG 4 cut(s) 573, 676, 699, 966
AsuHPI GGTGA 4 cut(s) 890, 1621, 2031, 2135
AvaII GGWCC 5 cut(s) 331, 672, 694, 908, 1987
BaeGI GKGCMC 1 cut(s) 1111
BanI GGYRCC 1 cut(s) 1090
BanII GRGCYC 2 cut(s) 285, 1946
BauI CACGAG 1 cut(s) 1080
BbrPI CACGTG 1 cut(s) 1112
BbvI GCAGC 4 cut(s) 1523, 1703, 2293, 2369
BccI CCATC 6 cut(s) 110, 236, 505, 1001, 2236, 2428
BciT130I CCWGG 3 cut(s) 171, 876, 989
BciVI GTATCC 2 cut(s) 400, 695
BclI TGATCA 1 cut(s) 1459
BcnI CCSGG 4 cut(s) 573, 676, 699, 966
BcoDI GTCTC 2 cut(s) 243, 373
BfaI CTAG 8 cut(s) 83, 155, 800, 1364, 1851, 2345, 2409, 2442
BfmI CTRYAG 2 cut(s) 868, 1533
BfuI GTATCC 2 cut(s) 400, 695
BglII AGATCT 2 cut(s) 1450, 1903
BisI GCNGC 5 cut(s) 1512, 1515, 1717, 2307, 2358
BlsI GCNGC 5 cut(s) 1513, 1516, 1718, 2308, 2359
BmcAI AGTACT 1 cut(s) 2431
Bme1390I CCNGG 7 cut(s) 171, 573, 676, 699, 876, 966, 989
Bme18I GGWCC 5 cut(s) 331, 672, 694, 908, 1987
BmgBI CACGTC 1 cut(s) 1225
BmgT120I GGNCC 5 cut(s) 331, 672, 694, 908, 1987
BmiI GGNNCC 6 cut(s) 542, 695, 696, 1092, 1694, 1988
BmrFI CCNGG 7 cut(s) 171, 573, 676, 699, 876, 966, 989
BmsI GCATC 7 cut(s) 76, 927, 1010, 1728, 1921, 2258, 2280
BplI GAGNNNNNCTC 2 cut(s) 267, 299
BpmI CTGGAG 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 4 cut(s) 573, 676, 699, 966
Bsa29I ATCGAT 1 cut(s) 500
BsaAI YACGTR 2 cut(s) 706, 1112
BsaBI GATNNNNATC 2 cut(s) 996, 1742
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 8 cut(s) 169, 697, 874, 987, 1357, 1518, 2211, 2232
BsaWI WCCGGW 1 cut(s) 2135
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 2389, 2419
Bsc4I CCNNNNNNNGG 9 cut(s) 234, 272, 348, 402, 578, 579, 993, 1057, 2234
Bse118I RCCGGY 2 cut(s) 519, 2313
Bse1I ACTGG 2 cut(s) 1914, 2125
Bse3DI GCAATG 2 cut(s) 2058, 2212
Bse8I GATNNNNATC 2 cut(s) 996, 1742
BseBI CCWGG 3 cut(s) 171, 876, 989
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 8 cut(s) 169, 697, 874, 987, 1357, 1518, 2211, 2232
BseGI GGATG 4 cut(s) 1025, 1936, 2228, 2271
BseJI GATNNNNATC 2 cut(s) 996, 1742
BseLI CCNNNNNNNGG 9 cut(s) 234, 272, 348, 402, 578, 579, 993, 1057, 2234
BseMI GCAATG 2 cut(s) 2058, 2212
BseMII CTCAG 5 cut(s) 600, 792, 1016, 1118, 1491
BseNI ACTGG 2 cut(s) 1914, 2125
BseRI GAGGAG 1 cut(s) 1218
BseSI GKGCMC 1 cut(s) 1111
BseXI GCAGC 4 cut(s) 1523, 1703, 2293, 2369
BseYI CCCAGC 3 cut(s) 278, 604, 2392
BsgI GTGCAG 1 cut(s) 1209
Bsh1236I CGCG 1 cut(s) 1184
BshFI GGCC 4 cut(s) 312, 1362, 1913, 2317
BshNI GGYRCC 1 cut(s) 1090
BshVI ATCGAT 1 cut(s) 500
BsiSI CCGG 7 cut(s) 520, 573, 675, 699, 966, 2136, 2314
BslFI GGGAC 6 cut(s) 544, 680, 967, 1248, 2000, 2201
BslI CCNNNNNNNGG 9 cut(s) 234, 272, 348, 402, 578, 579, 993, 1057, 2234
BsmAI GTCTC 2 cut(s) 243, 373
BsmFI GGGAC 6 cut(s) 544, 680, 967, 1248, 2000, 2201
BsnI GGCC 4 cut(s) 312, 1362, 1913, 2317
Bso31I GGTCTC 1 cut(s) 373
Bsp1286I GDGCHC 3 cut(s) 285, 1111, 1946
Bsp1407I TGTACA 2 cut(s) 2085, 2465
Bsp19I CCATGG 1 cut(s) 2232
BspACI CCGC 7 cut(s) 523, 617, 794, 1344, 1515, 1607, 2330
BspANI GGCC 4 cut(s) 312, 1362, 1913, 2317
BspCNI CTCAG 5 cut(s) 601, 791, 1017, 1119, 1490
BspDI ATCGAT 1 cut(s) 500
BspFNI CGCG 1 cut(s) 1184
BspHI TCATGA 1 cut(s) 841
BspLI GGNNCC 6 cut(s) 542, 695, 696, 1092, 1694, 1988
BspPI GGATC 5 cut(s) 563, 583, 863, 2219, 2411
BspQI GCTCTTC 1 cut(s) 1391
BspT107I GGYRCC 1 cut(s) 1090
BspTNI GGTCTC 1 cut(s) 373
BsrBI CCGCTC 2 cut(s) 794, 1346
BsrDI GCAATG 2 cut(s) 2058, 2212
BsrFI RCCGGY 2 cut(s) 519, 2313
BsrGI TGTACA 2 cut(s) 2085, 2465
BsrI ACTGG 2 cut(s) 1914, 2125
BssAI RCCGGY 2 cut(s) 519, 2313
BssECI CCNNGG 8 cut(s) 169, 697, 874, 987, 1357, 1518, 2211, 2232
BssSI CACGAG 1 cut(s) 1080
BssT1I CCWWGG 3 cut(s) 1357, 2211, 2232
Bst2BI CACGAG 1 cut(s) 1080
Bst2UI CCWGG 3 cut(s) 171, 876, 989
Bst4CI ACNGT 7 cut(s) 367, 490, 825, 903, 1507, 1570, 2090
Bst6I CTCTTC 5 cut(s) 70, 376, 1391, 1641, 1725
BstAUI TGTACA 2 cut(s) 2085, 2465
BstBAI YACGTR 2 cut(s) 706, 1112
BstC8I GCNNGC 2 cut(s) 1326, 2382
BstDEI CTNAG 7 cut(s) 110, 286, 609, 778, 1025, 1127, 1477
BstDSI CCRYGG 2 cut(s) 1518, 2232
BstF5I GGATG 4 cut(s) 1025, 1936, 2228, 2271
BstFNI CGCG 1 cut(s) 1184
BstMAI GTCTC 2 cut(s) 243, 373
BstMWI GCNNNNNNNGC 6 cut(s) 86, 165, 520, 529, 538, 1325
BstNI CCWGG 3 cut(s) 171, 876, 989
BstNSI RCATGY 2 cut(s) 1050, 2466
BstSCI CCNGG 7 cut(s) 169, 571, 674, 697, 874, 964, 987
BstSFI CTRYAG 2 cut(s) 868, 1533
BstSLI GKGCMC 1 cut(s) 1111
BstSNI TACGTA 1 cut(s) 706
BstUI CGCG 1 cut(s) 1184
BstV1I GCAGC 4 cut(s) 1523, 1703, 2293, 2369
BstX2I RGATCY 3 cut(s) 1450, 1903, 2416
BstYI RGATCY 3 cut(s) 1450, 1903, 2416
Bsu15I ATCGAT 1 cut(s) 500
BsuI GTATCC 2 cut(s) 400, 695
BsuRI GGCC 4 cut(s) 312, 1362, 1913, 2317
BsuTUI ATCGAT 1 cut(s) 500
BtgI CCRYGG 2 cut(s) 1518, 2232
BtrI CACGTC 1 cut(s) 1225
BtsCI GGATG 4 cut(s) 1025, 1936, 2228, 2271
BtsIMutI CAGTG 2 cut(s) 495, 908
Cac8I GCNNGC 2 cut(s) 1326, 2382
CciI TCATGA 1 cut(s) 841
Cfr10I RCCGGY 2 cut(s) 519, 2313
Cfr13I GGNCC 5 cut(s) 331, 672, 694, 908, 1987
ClaI ATCGAT 1 cut(s) 500
CseI GACGC 2 cut(s) 1029, 1923
DdeI CTNAG 7 cut(s) 110, 286, 609, 778, 1025, 1127, 1477
DrdI GACNNNNNNGTC 1 cut(s) 906
DseDI GACNNNNNNGTC 1 cut(s) 906
EaeI YGGCCR 1 cut(s) 2315
Eam1104I CTCTTC 5 cut(s) 70, 376, 1391, 1641, 1725
EarI CTCTTC 5 cut(s) 70, 376, 1391, 1641, 1725
Eco105I TACGTA 1 cut(s) 706
Eco130I CCWWGG 3 cut(s) 1357, 2211, 2232
Eco147I AGGCCT 1 cut(s) 312
Eco24I GRGCYC 2 cut(s) 285, 1946
Eco31I GGTCTC 1 cut(s) 373
Eco47I GGWCC 5 cut(s) 331, 672, 694, 908, 1987
Eco57I CTGAAG 2 cut(s) 554, 2454
Eco72I CACGTG 1 cut(s) 1112
EcoO109I RGGNCCY 1 cut(s) 694
EcoRI GAATTC 4 cut(s) 4, 813, 1627, 2131
EcoRII CCWGG 3 cut(s) 169, 874, 987
EcoT14I CCWWGG 3 cut(s) 1357, 2211, 2232
EcoT22I ATGCAT 1 cut(s) 2273
EcoT38I GRGCYC 2 cut(s) 285, 1946
ErhI CCWWGG 3 cut(s) 1357, 2211, 2232
FaqI GGGAC 6 cut(s) 544, 680, 967, 1248, 2000, 2201
FauNDI CATATG 1 cut(s) 2026
FbaI TGATCA 1 cut(s) 1459
FblI GTMKAC 1 cut(s) 1043
Fnu4HI GCNGC 5 cut(s) 1512, 1515, 1717, 2307, 2358
FokI GGATG 4 cut(s) 1032, 1943, 2215, 2258
FriOI GRGCYC 2 cut(s) 285, 1946
Fsp4HI GCNGC 5 cut(s) 1512, 1515, 1717, 2307, 2358
FspBI CTAG 8 cut(s) 83, 155, 800, 1364, 1851, 2345, 2409, 2442
GluI GCNGC 5 cut(s) 1512, 1515, 1717, 2307, 2358
GsaI CCCAGC 3 cut(s) 282, 608, 2396
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 4 cut(s) 312, 1362, 1913, 2317
HapII CCGG 7 cut(s) 520, 573, 675, 699, 966, 2136, 2314
HgaI GACGC 2 cut(s) 1029, 1923
HincII GTYRAC 1 cut(s) 2254
HindII GTYRAC 1 cut(s) 2254
HindIII AAGCTT 2 cut(s) 724, 1544
HpaII CCGG 7 cut(s) 520, 573, 675, 699, 966, 2136, 2314
HphI GGTGA 4 cut(s) 890, 1621, 2031, 2135
Hpy166II GTNNAC 5 cut(s) 733, 1044, 1583, 2086, 2254
Hpy188I TCNGA 7 cut(s) 665, 722, 1002, 1128, 1480, 2021, 2080
Hpy188III TCNNGA 8 cut(s) 235, 810, 842, 1035, 1454, 1702, 1874, 1881
Hpy8I GTNNAC 5 cut(s) 733, 1044, 1583, 2086, 2254
Hpy99I CGWCG 3 cut(s) 125, 645, 1511
HpyAV CCTTC 9 cut(s) 322, 1055, 1081, 1205, 2051, 2121, 2195, 2376, 2429
HpyCH4III ACNGT 7 cut(s) 367, 490, 825, 903, 1507, 1570, 2090
HpyCH4IV ACGT 4 cut(s) 123, 705, 1111, 1224
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 165, 520, 529, 538, 1325
HpyF3I CTNAG 7 cut(s) 110, 286, 609, 778, 1025, 1127, 1477
HpySE526I ACGT 4 cut(s) 123, 705, 1111, 1224
KflI GGGWCCC 1 cut(s) 694
KpnI GGTACC 1 cut(s) 1094
Ksp22I TGATCA 1 cut(s) 1459
LguI GCTCTTC 1 cut(s) 1391
LmnI GCTCC 4 cut(s) 275, 546, 1037, 2438
Lsp1109I GCAGC 4 cut(s) 1523, 1703, 2293, 2369
LweI GCATC 7 cut(s) 76, 927, 1010, 1728, 1921, 2258, 2280
MaeI CTAG 8 cut(s) 83, 155, 800, 1364, 1851, 2345, 2409, 2442
MaeII ACGT 4 cut(s) 123, 705, 1111, 1224
MaeIII GTNAC 9 cut(s) 361, 443, 766, 887, 923, 1220, 2037, 2348, 2458
MbiI CCGCTC 2 cut(s) 794, 1346
MflI RGATCY 3 cut(s) 1450, 1903, 2416
MhlI GDGCHC 3 cut(s) 285, 1111, 1946
MlyI GAGTC 5 cut(s) 669, 1091, 1132, 1138, 1769
MmeI TCCRAC 4 cut(s) 643, 1708, 1999, 2155
Mph1103I ATGCAT 1 cut(s) 2273
MroXI GAANNNNTTC 4 cut(s) 654, 1256, 2065, 2131
MseI TTAA 4 cut(s) 819, 1071, 1653, 1886
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 7 cut(s) 520, 573, 675, 699, 966, 2136, 2314
MspR9I CCNGG 7 cut(s) 171, 573, 676, 699, 876, 966, 989
MvaI CCWGG 3 cut(s) 171, 876, 989
MvnI CGCG 1 cut(s) 1184
MwoI GCNNNNNNNGC 6 cut(s) 86, 165, 520, 529, 538, 1325
NciI CCSGG 4 cut(s) 573, 676, 699, 966
NcoI CCATGG 1 cut(s) 2232
NdeI CATATG 1 cut(s) 2026
NlaIV GGNNCC 6 cut(s) 542, 695, 696, 1092, 1694, 1988
NmuCI GTSAC 7 cut(s) 361, 766, 887, 923, 1220, 2037, 2458
NsiI ATGCAT 1 cut(s) 2273
NspI RCATGY 2 cut(s) 1050, 2466
PagI TCATGA 1 cut(s) 841
PceI AGGCCT 1 cut(s) 312
PciI ACATGT 1 cut(s) 2462
PciSI GCTCTTC 1 cut(s) 1391
PdmI GAANNNNTTC 4 cut(s) 654, 1256, 2065, 2131
PfeI GAWTC 5 cut(s) 55, 806, 1877, 2240, 2401
PflMI CCANNNNNTGG 2 cut(s) 234, 402
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 5 cut(s) 1513, 1516, 1718, 2308, 2359
PleI GAGTC 5 cut(s) 668, 1091, 1131, 1137, 1769
PmaCI CACGTG 1 cut(s) 1112
PmlI CACGTG 1 cut(s) 1112
PpsI GAGTC 5 cut(s) 668, 1091, 1131, 1137, 1769
Ppu21I YACGTR 2 cut(s) 706, 1112
PpuMI RGGWCCY 1 cut(s) 694
PscI ACATGT 1 cut(s) 2462
PsiI TTATAA 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 3 cut(s) 169, 874, 987
PspCI CACGTG 1 cut(s) 1112
PspFI CCCAGC 3 cut(s) 278, 604, 2392
PspGI CCWGG 3 cut(s) 169, 874, 987
PspN4I GGNNCC 6 cut(s) 542, 695, 696, 1092, 1694, 1988
PspPI GGNCC 5 cut(s) 331, 672, 694, 908, 1987
PspPPI RGGWCCY 1 cut(s) 694
PsuI RGATCY 3 cut(s) 1450, 1903, 2416
PvuII CAGCTG 1 cut(s) 608
SapI GCTCTTC 1 cut(s) 1391
SaqAI TTAA 4 cut(s) 819, 1071, 1653, 1886
SatI GCNGC 5 cut(s) 1512, 1515, 1717, 2307, 2358
Sau96I GGNCC 5 cut(s) 331, 672, 694, 908, 1987
ScaI AGTACT 1 cut(s) 2431
SchI GAGTC 5 cut(s) 669, 1091, 1132, 1138, 1769
ScrFI CCNGG 7 cut(s) 171, 573, 676, 699, 876, 966, 989
SduI GDGCHC 3 cut(s) 285, 1111, 1946
SfaNI GCATC 7 cut(s) 76, 927, 1010, 1728, 1921, 2258, 2280
SfcI CTRYAG 2 cut(s) 868, 1533
SinI GGWCC 5 cut(s) 331, 672, 694, 908, 1987
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SnaBI TACGTA 1 cut(s) 706
SseBI AGGCCT 1 cut(s) 312
SsiI CCGC 7 cut(s) 523, 617, 794, 1344, 1515, 1607, 2330
SspI AATATT 2 cut(s) 1921, 1977
SspMI CTAG 8 cut(s) 83, 155, 800, 1364, 1851, 2345, 2409, 2442
StuI AGGCCT 1 cut(s) 312
StyD4I CCNGG 7 cut(s) 169, 571, 674, 697, 874, 964, 987
StyI CCWWGG 3 cut(s) 1357, 2211, 2232
TaaI ACNGT 7 cut(s) 367, 490, 825, 903, 1507, 1570, 2090
TaiI ACGT 4 cut(s) 126, 708, 1114, 1227
TaqI TCGA 8 cut(s) 74, 500, 643, 658, 858, 1455, 1622, 1880
TatI WGTACW 4 cut(s) 1582, 2085, 2429, 2465
TauI GCSGC 1 cut(s) 1517
TfiI GAWTC 5 cut(s) 55, 806, 1877, 2240, 2401
Tru1I TTAA 4 cut(s) 819, 1071, 1653, 1886
Tru9I TTAA 4 cut(s) 819, 1071, 1653, 1886
TscAI CASTG 2 cut(s) 495, 908
TseFI GTSAC 7 cut(s) 361, 766, 887, 923, 1220, 2037, 2458
TseI GCWGC 4 cut(s) 1511, 1716, 2306, 2357
Tsp45I GTSAC 7 cut(s) 361, 766, 887, 923, 1220, 2037, 2458
TspGWI ACGGA 2 cut(s) 376, 1535
TspRI CASTG 2 cut(s) 495, 908
Van91I CCANNNNNTGG 2 cut(s) 234, 402
VpaK11BI GGWCC 5 cut(s) 331, 672, 694, 908, 1987
XapI RAATTY 9 cut(s) 4, 14, 213, 583, 813, 1627, 2061, 2131, 2167
XceI RCATGY 2 cut(s) 1050, 2466
XmiI GTMKAC 1 cut(s) 1043
XmnI GAANNNNTTC 4 cut(s) 654, 1256, 2065, 2131
XspI CTAG 8 cut(s) 83, 155, 800, 1364, 1851, 2345, 2409, 2442
ZrmI AGTACT 1 cut(s) 2431
Zsp2I ATGCAT 1 cut(s) 2273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.