Rw2G022660

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000099
Physical Location & Seq
Forward (+)
32502058 .. 32505445
3388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022660.1

Sequence Viewer

Length: 2514 bp
ATGAATTCTTTTCAATGGTCTATGATCACTGCATTGCTCATCTTCCTTCTTTATCTCCCCTCTTGCATTTCCATTGATTCCATTACTCCAAACCAAACCATCAAAGATGGCGACGTTTTAGTCTCAAGCAGGAAGCTCTTTGCTCTAGGGTTCTTCAGCCCTGGAAATTCTGGCAAACGTTACATTGGAGTTTGGTTTAACAAAGTTCCAGAGCAGACCATTGTTTGGGTAGCAAATAGAGACAACCCTGTCAATGATTCCTCTGGACTTCTAGTGGTCGATGCAGATGGAGGCCTTGTCATATATGGAAAGGACAGAAAACTCCCTCTTTGGTCGGCTAATGTTACTCTCTCTTCTCCAAAAAACTCCATGGCCAAGCTGTTGGATACAGGTAACTTCATTTTGTTTGAGAATGGGCGGAGCCTGTGGGAAGGGTTTGATTATCCCTCAAATACAATGCTTCCCTTTATGAAAATTGGACTGAACCGGCGATCCGAGCTTAACCGGTTCCTAACTTCTTGGAAGTCCAAAGATGACCCGGGAACTGGGAATTTCTCAGCTAGGATTGATCCGGATGGGTTTCCGCAGCTGTTTTTGTACAACGGTCAAGTTCCCCGATGGCGGGCCGGATCTTGGATCGGACACAGATGGAGTGGTATCCCCGAAATGACTCGTAATTTCATCTTCAATACTGGTTTTGTGACCAATCAAGATGAGCCATCCGTCATGACTGGCATTAAGGACGACTCAATCTTCTCAAGGATGGTGCTTGATGAATCGGGAATCATCGAACGGTCCATGTGGCAAGATCAGGATCACCGATGGAACAAGTACTGGCATGCCCCGGCAGAACTATGTGACGACTATGGAAAGTGTGGTCCAAATAGCAACTGTGACCCTTACAATGCGGATGAGTTCGAGTGTACATGCCTACCTGGGTTTGAACCTAAGTTGCCTCGTGCATGGTACTTGAGAGATGGCTCGGATGGGTGTGTGAGGAAAAAAGGAGCATCCGTGTGTAAAAACGGAGAAGGGTTCGTGAAGGTGTCACCAGTAAAGGCACCAGACTCATCTACTGCGCATGTAAACTTGACCATGGGTCCGAAAGAATGTGAAGAGGAATGCATAAGGAATTGTTCTTGCATGGCATACTCGGGTGCAGGTGACCCGCAAGAAAGGAATGGATGTGTGACATGGTACGGGGACTTGATGGATACAAGGACTTACACGATTGTCACTCAAGAATTATATGTTCGAGTTGATTCAATTGAATTAGCTAAACACGCAAGGAAGTCGAATGGTTCTTTTAGAAAGAAGGGAAAGCTGGCAATTACTATAACATCTATTTTAGTGTCCTTTCTTGTGATCTCTATAGCATATTGGTCCGTAAAGAAGAAGAGAAAAGGTAAGGAAAGACAAAATATATTTCCATTTGGTGTGATCACACCTTCAATCTACTTGGAAGATTCTCCTAGTAGAACAGATGCTGATGATAGCAGATTAAAGCCGGATCTACCTTTCTTTGATCTAAGCACCATATCTGCAGCCACAAAAAATTTCTCTGATTCGAACAGGCTTGGAGAAGGCGGATTCGGCCCCGTTTATAAGGGAGTGCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTTAGCAAAAATTCTGGCCAGGGAAATGAAGAGTTCAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTGAGAATCTTAGGCTATTGCGTTCAAGATGAAGAGAAGATGCTAATCTATGAATACCTGCCCAACAAAAGCTTGGACTCGTTCATATTCAATGAAATGAAAAGGACATCTCTGGATTGGACACGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGGATCTTGTATCTTCATCAAGATTCGAGATTAAGAATTATCCACAGAGACCTAAAGGCCAGCAATATTCTACTGGATTATGATATGAACCCCAAACTTGCAGATTTTGGTATGGCTAGAATATTCAGAGCAAACCAAATTGAAGCAAATACAAATCGCGTGGTTGGAACATATGGTTACATGTCACCGGAGTATGCAATGGAAGGACTATTTTCAATGAAGTCTGATGTCTACAGTTTCGGTGTTTTGCTGCTAGAAATCGTTACTGGTAGAAAGAACACCGGTTACTACTATGATAATCCTGACTCAAATTTAGTTGGACATGTTTGGGACTTGTGGAAAGAAGGCCGAGCCTTGGAAATCGTCGACCCATCTTTGGGAGAATCCTACCCCATAAGTGAAGTTCTACGGTGCATTCAAATCGCACTCCTGTGCGTGCAAGAGTACGCAAATGACCGGCCAACCATGTCAAAAGTTGTGTCTGTGTTAGGCAACGATGCAGCTCTTCCTTCACCAAGAAAACCTGGATTTCTCTTCAGAAGTAACTATACCAATGGAGACCCCAGTACTAGTACTGCAGATGCTAATTCCATAAATTATGTTACATGCAGTATAGTTGAAGCTCGCTAA

Protein Analysis

837

Amino Acids

94.19

Weight (kDa)

7.42

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 175 3e-30 D-mannose binding lectin
S_locus_glycop PF00954 207 - 317 5.8e-28 S-locus glycoprotein domain
PAN_2 PF08276 340 - 406 1.9e-17 PAN-like domain
Pkinase PF00069 522 - 788 7.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 524 - 790 1.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1605
AarI CACCTGC 1 cut(s) 1151
AasI GACNNNNNNGTC 2 cut(s) 119, 248
Acc16I TGCGCA 1 cut(s) 1080
Acc36I ACCTGC 2 cut(s) 1151, 1794
AccB1I GGYRCC 1 cut(s) 1060
AccB7I CCANNNNNTGG 1 cut(s) 225
AccI GTMKAC 2 cut(s) 2115, 2250
AccII CGCG 1 cut(s) 2043
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 6 cut(s) 418, 584, 622, 908, 1169, 1587
AclI AACGTT 1 cut(s) 178
AclWI GGATC 7 cut(s) 486, 563, 637, 644, 822, 1518, 1895
AcoI YGGCCR 3 cut(s) 372, 1663, 2342
AcsI RAATTY 6 cut(s) 4, 166, 550, 1555, 1657, 2194
AcuI CTGAAG 2 cut(s) 139, 2404
AfaI GTAC 8 cut(s) 599, 833, 925, 968, 1199, 2330, 2452, 2458
AfiI CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2239, 2260, 2261
AflIII ACRYGT 2 cut(s) 2064, 2206
AgeI ACCGGT 2 cut(s) 504, 2165
AhdI GACNNNNNGTC 1 cut(s) 1098
AhlI ACTAGT 1 cut(s) 2453
AjnI CCWGG 4 cut(s) 160, 934, 1665, 2407
AleI CACNNNNGTG 1 cut(s) 2314
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
Alw26I GTCTC 4 cut(s) 127, 234, 1926, 2436
AlwI GGATC 7 cut(s) 486, 563, 637, 644, 822, 1518, 1895
AlwNI CAGNNNCTG 1 cut(s) 1487
Ama87I CYCGRG 2 cut(s) 538, 1153
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 8 cut(s) 292, 372, 624, 1594, 1663, 1941, 2230, 2342
ApeKI GCWGC 4 cut(s) 586, 1544, 2134, 2384
ApoI RAATTY 6 cut(s) 4, 166, 550, 1555, 1657, 2194
ArsI GACNNNNNNTTYG 2 cut(s) 2348, 2380
AsiGI ACCGGT 2 cut(s) 504, 2165
AspLEI GCGC 1 cut(s) 1081
AspS9I GGNCC 6 cut(s) 624, 795, 878, 1100, 1383, 1595
AsuC2I CCSGG 3 cut(s) 539, 540, 845
AsuHPI GGTGA 5 cut(s) 809, 1041, 1175, 2061, 2388
AsuII TTCGAA 1 cut(s) 1568
AvaI CYCGRG 2 cut(s) 538, 1153
AvaII GGWCC 4 cut(s) 795, 878, 1100, 1383
BalI TGGCCA 2 cut(s) 374, 1665
BanI GGYRCC 1 cut(s) 1060
BarI GAAGNNNNNNTAC 2 cut(s) 2416, 2448
BauI CACGAG 1 cut(s) 957
BbvI GCAGC 4 cut(s) 598, 1556, 2121, 2396
BceAI ACGGC 1 cut(s) 1622
BcgI CGANNNNNNTGC 4 cut(s) 1275, 1309, 2287, 2321
BciT130I CCWGG 4 cut(s) 162, 936, 1667, 2409
BciVI GTATCC 3 cut(s) 379, 668, 1207
BclI TGATCA 2 cut(s) 24, 1440
BcnI CCSGG 3 cut(s) 539, 540, 845
BcoDI GTCTC 4 cut(s) 127, 234, 1926, 2436
BcuI ACTAGT 1 cut(s) 2453
BfaI CTAG 9 cut(s) 146, 272, 561, 1473, 1620, 1881, 2001, 2138, 2454
BfmI CTRYAG 4 cut(s) 1371, 1542, 2116, 2460
BfuAI ACCTGC 2 cut(s) 1151, 1794
BfuI GTATCC 3 cut(s) 379, 668, 1207
BisI GCNGC 4 cut(s) 587, 1545, 2135, 2385
BlsI GCNGC 4 cut(s) 588, 1546, 2136, 2386
BmcAI AGTACT 3 cut(s) 833, 2452, 2458
Bme1390I CCNGG 7 cut(s) 162, 539, 540, 845, 936, 1667, 2409
Bme18I GGWCC 4 cut(s) 795, 878, 1100, 1383
BmeRI GACNNNNNGTC 1 cut(s) 1098
BmeT110I CYCGRG 2 cut(s) 538, 1153
BmgT120I GGNCC 6 cut(s) 624, 795, 878, 1100, 1383, 1595
BmiI GGNNCC 6 cut(s) 422, 509, 1062, 1101, 1597, 1724
BmrFI CCNGG 7 cut(s) 162, 539, 540, 845, 936, 1667, 2409
BmrI ACTGGG 2 cut(s) 555, 2442
BmsI GCATC 6 cut(s) 271, 1019, 1474, 1758, 2371, 2455
BmuI ACTGGG 2 cut(s) 555, 2442
Bpu14I TTCGAA 1 cut(s) 1568
BpuEI CTTGAG 4 cut(s) 109, 742, 991, 1224
BpuMI CCSGG 3 cut(s) 539, 540, 845
BsaBI GATNNNNATC 2 cut(s) 813, 1772
BsaI GGTCTC 2 cut(s) 1926, 2436
BsaJI CCNNGG 8 cut(s) 160, 369, 538, 843, 935, 1095, 1666, 2238
BsaWI WCCGGW 4 cut(s) 504, 571, 2071, 2165
BsaXI ACNNNNNCTCC 4 cut(s) 282, 312, 999, 1029
Bsc4I CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2239, 2260, 2261
Bse118I RCCGGY 4 cut(s) 486, 504, 2165, 2340
Bse1I ACTGG 8 cut(s) 550, 697, 736, 839, 1052, 1962, 2155, 2448
Bse3DI GCAATG 3 cut(s) 32, 1875, 2088
Bse8I GATNNNNATC 2 cut(s) 813, 1772
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 4 cut(s) 162, 936, 1667, 2409
BseDI CCNNGG 8 cut(s) 160, 369, 538, 843, 935, 1095, 1666, 2238
BseGI GGATG 7 cut(s) 580, 719, 768, 916, 991, 1010, 1190
BseJI GATNNNNATC 2 cut(s) 813, 1772
BseLI CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2239, 2260, 2261
BseMI GCAATG 3 cut(s) 32, 1875, 2088
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 8 cut(s) 550, 697, 736, 839, 1052, 1962, 2155, 2448
BseXI GCAGC 4 cut(s) 598, 1556, 2121, 2396
BsgI GTGCAG 1 cut(s) 1179
Bsh1236I CGCG 1 cut(s) 2043
BshFI GGCC 8 cut(s) 294, 374, 626, 1596, 1665, 1943, 2232, 2344
BshNI GGYRCC 1 cut(s) 1060
BshTI ACCGGT 2 cut(s) 504, 2165
BsiHKCI CYCGRG 2 cut(s) 538, 1153
BslFI GGGAC 2 cut(s) 1217, 2228
BslI CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2239, 2260, 2261
BsmAI GTCTC 4 cut(s) 127, 234, 1926, 2436
BsmFI GGGAC 2 cut(s) 1217, 2228
BsmI GAATGC 2 cut(s) 1127, 2298
BsnI GGCC 8 cut(s) 294, 374, 626, 1596, 1665, 1943, 2232, 2344
Bso31I GGTCTC 2 cut(s) 1926, 2436
BsoBI CYCGRG 2 cut(s) 538, 1153
Bsp119I TTCGAA 1 cut(s) 1568
Bsp13I TCCGGA 1 cut(s) 571
Bsp1407I TGTACA 2 cut(s) 597, 923
Bsp19I CCATGG 2 cut(s) 369, 1095
BspACI CCGC 6 cut(s) 418, 584, 622, 908, 1169, 1587
BspANI GGCC 8 cut(s) 294, 374, 626, 1596, 1665, 1943, 2232, 2344
BspCNI CTCAG 1 cut(s) 569
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 1 cut(s) 2043
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 6 cut(s) 422, 509, 1062, 1101, 1597, 1724
BspMAI CTGCAG 2 cut(s) 1546, 2464
BspMI ACCTGC 2 cut(s) 1151, 1794
BspPI GGATC 7 cut(s) 486, 563, 637, 644, 822, 1518, 1895
BspQI GCTCTTC 1 cut(s) 2394
BspT104I TTCGAA 1 cut(s) 1568
BspT107I GGYRCC 1 cut(s) 1060
BspTNI GGTCTC 2 cut(s) 1926, 2436
BsrDI GCAATG 3 cut(s) 32, 1875, 2088
BsrFI RCCGGY 4 cut(s) 486, 504, 2165, 2340
BsrGI TGTACA 2 cut(s) 597, 923
BsrI ACTGG 8 cut(s) 550, 697, 736, 839, 1052, 1962, 2155, 2448
BssAI RCCGGY 4 cut(s) 486, 504, 2165, 2340
BssECI CCNNGG 8 cut(s) 160, 369, 538, 843, 935, 1095, 1666, 2238
BssSI CACGAG 1 cut(s) 957
BssT1I CCWWGG 3 cut(s) 369, 1095, 2238
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 4 cut(s) 162, 936, 1667, 2409
Bst4CI ACNGT 5 cut(s) 605, 795, 893, 2120, 2295
Bst6I CTCTTC 7 cut(s) 358, 1110, 1391, 1671, 1755, 2394, 2423
BstAUI TGTACA 2 cut(s) 597, 923
BstBI TTCGAA 1 cut(s) 1568
BstC8I GCNNGC 6 cut(s) 624, 840, 1326, 1945, 2321, 2509
BstDEI CTNAG 5 cut(s) 556, 948, 1529, 1649, 1738
BstDSI CCRYGG 2 cut(s) 369, 1095
BstEII GGTNACC 1 cut(s) 1163
BstF5I GGATG 7 cut(s) 580, 719, 768, 916, 991, 1010, 1190
BstFNI CGCG 1 cut(s) 2043
BstHHI GCGC 1 cut(s) 1081
BstMAI GTCTC 4 cut(s) 127, 234, 1926, 2436
BstMWI GCNNNNNNNGC 3 cut(s) 496, 1283, 1593
BstNI CCWGG 4 cut(s) 162, 936, 1667, 2409
BstNSI RCATGY 6 cut(s) 842, 930, 1085, 2068, 2210, 2493
BstPI GGTNACC 1 cut(s) 1163
BstSCI CCNGG 7 cut(s) 160, 537, 538, 843, 934, 1665, 2407
BstSFI CTRYAG 4 cut(s) 1371, 1542, 2116, 2460
BstUI CGCG 1 cut(s) 2043
BstV1I GCAGC 4 cut(s) 598, 1556, 2121, 2396
BstX2I RGATCY 3 cut(s) 629, 1510, 1887
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 3 cut(s) 629, 1510, 1887
BsuI GTATCC 3 cut(s) 379, 668, 1207
BsuRI GGCC 8 cut(s) 294, 374, 626, 1596, 1665, 1943, 2232, 2344
BtgI CCRYGG 2 cut(s) 369, 1095
BtsCI GGATG 7 cut(s) 580, 719, 768, 916, 991, 1010, 1190
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
BveI ACCTGC 2 cut(s) 1151, 1794
Cac8I GCNNGC 6 cut(s) 624, 840, 1326, 1945, 2321, 2509
CaiI CAGNNNCTG 1 cut(s) 1487
CciI TCATGA 1 cut(s) 726
CfoI GCGC 1 cut(s) 1081
Cfr10I RCCGGY 4 cut(s) 486, 504, 2165, 2340
Cfr13I GGNCC 6 cut(s) 624, 795, 878, 1100, 1383, 1595
Cfr9I CCCGGG 1 cut(s) 538
CseI GACGC 1 cut(s) 1863
Csp6I GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2329, 2451, 2457
CspAI ACCGGT 2 cut(s) 504, 2165
CspCI CAANNNNNGTGG 2 cut(s) 2025, 2060
CviQI GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2329, 2451, 2457
DdeI CTNAG 5 cut(s) 556, 948, 1529, 1649, 1738
DrdI GACNNNNNNGTC 2 cut(s) 119, 248
DriI GACNNNNNGTC 1 cut(s) 1098
DseDI GACNNNNNNGTC 2 cut(s) 119, 248
EaeI YGGCCR 3 cut(s) 372, 1663, 2342
Eam1104I CTCTTC 7 cut(s) 358, 1110, 1391, 1671, 1755, 2394, 2423
Eam1105I GACNNNNNGTC 1 cut(s) 1098
EarI CTCTTC 7 cut(s) 358, 1110, 1391, 1671, 1755, 2394, 2423
EciI GGCGGA 2 cut(s) 433, 1602
Eco130I CCWWGG 3 cut(s) 369, 1095, 2238
Eco147I AGGCCT 1 cut(s) 294
Eco31I GGTCTC 2 cut(s) 1926, 2436
Eco47I GGWCC 4 cut(s) 795, 878, 1100, 1383
Eco57I CTGAAG 2 cut(s) 139, 2404
Eco88I CYCGRG 2 cut(s) 538, 1153
Eco91I GGTNACC 1 cut(s) 1163
EcoO65I GGTNACC 1 cut(s) 1163
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 4 cut(s) 160, 934, 1665, 2407
EcoT14I CCWWGG 3 cut(s) 369, 1095, 2238
EcoT22I ATGCAT 1 cut(s) 1127
ErhI CCWWGG 3 cut(s) 369, 1095, 2238
FaqI GGGAC 2 cut(s) 1217, 2228
FauI CCCGC 2 cut(s) 615, 1176
FauNDI CATATG 1 cut(s) 2056
FbaI TGATCA 2 cut(s) 24, 1440
FblI GTMKAC 2 cut(s) 2115, 2250
Fnu4HI GCNGC 4 cut(s) 587, 1545, 2135, 2385
FokI GGATG 7 cut(s) 587, 706, 775, 923, 997, 998, 1197
Fsp4HI GCNGC 4 cut(s) 587, 1545, 2135, 2385
FspBI CTAG 9 cut(s) 146, 272, 561, 1473, 1620, 1881, 2001, 2138, 2454
FspI TGCGCA 1 cut(s) 1080
GlaI GCGC 1 cut(s) 1080
GluI GCNGC 4 cut(s) 587, 1545, 2135, 2385
HaeIII GGCC 8 cut(s) 294, 374, 626, 1596, 1665, 1943, 2232, 2344
HgaI GACGC 1 cut(s) 1863
HhaI GCGC 1 cut(s) 1081
Hin6I GCGC 1 cut(s) 1079
HinP1I GCGC 1 cut(s) 1079
HincII GTYRAC 1 cut(s) 2251
HindII GTYRAC 1 cut(s) 2251
HindIII AAGCTT 1 cut(s) 1798
HphI GGTGA 5 cut(s) 809, 1041, 1175, 2061, 2388
Hpy166II GTNNAC 4 cut(s) 924, 1087, 2116, 2251
Hpy188I TCNGA 8 cut(s) 496, 641, 985, 1104, 1564, 2012, 2110, 2423
Hpy8I GTNNAC 4 cut(s) 924, 1087, 2116, 2251
Hpy99I CGWCG 3 cut(s) 116, 1857, 2252
HpyCH4III ACNGT 5 cut(s) 605, 795, 893, 2120, 2295
HpyCH4IV ACGT 2 cut(s) 114, 178
HpyF10VI GCNNNNNNNGC 3 cut(s) 496, 1283, 1593
HpyF3I CTNAG 5 cut(s) 556, 948, 1529, 1649, 1738
HpySE526I ACGT 2 cut(s) 114, 178
HspAI GCGC 1 cut(s) 1079
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 2 cut(s) 24, 1440
LguI GCTCTTC 1 cut(s) 2394
LmnI GCTCC 2 cut(s) 420, 1007
Lsp1109I GCAGC 4 cut(s) 598, 1556, 2121, 2396
LweI GCATC 6 cut(s) 271, 1019, 1474, 1758, 2371, 2455
MaeI CTAG 9 cut(s) 146, 272, 561, 1473, 1620, 1881, 2001, 2138, 2454
MaeII ACGT 2 cut(s) 114, 178
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 3 cut(s) 629, 1510, 1887
MlsI TGGCCA 2 cut(s) 374, 1665
MluNI TGGCCA 2 cut(s) 374, 1665
MlyI GAGTC 5 cut(s) 664, 740, 1061, 1799, 2183
MmeI TCCRAC 3 cut(s) 363, 2029, 2182
MnlI CCTC 9 cut(s) 70, 271, 284, 336, 457, 966, 990, 1111, 1877
Mox20I TGGCCA 2 cut(s) 374, 1665
Mph1103I ATGCAT 1 cut(s) 1127
MroI TCCGGA 1 cut(s) 571
MscI TGGCCA 2 cut(s) 374, 1665
MseI TTAA 5 cut(s) 198, 501, 738, 1502, 1916
MslI CAYNNNNRTG 2 cut(s) 1015, 2314
Msp20I TGGCCA 2 cut(s) 374, 1665
MspA1I CMGCKG 1 cut(s) 589
MspR9I CCNGG 7 cut(s) 162, 539, 540, 845, 936, 1667, 2409
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 2 cut(s) 1127, 2298
MvaI CCWGG 4 cut(s) 162, 936, 1667, 2409
MvnI CGCG 1 cut(s) 2043
MwoI GCNNNNNNNGC 3 cut(s) 496, 1283, 1593
NciI CCSGG 3 cut(s) 539, 540, 845
NcoI CCATGG 2 cut(s) 369, 1095
NdeI CATATG 1 cut(s) 2056
NlaIV GGNNCC 6 cut(s) 422, 509, 1062, 1101, 1597, 1724
NmeAIII GCCGAG 1 cut(s) 2258
NmuCI GTSAC 8 cut(s) 700, 857, 893, 1047, 1163, 1189, 1234, 2067
NsbI TGCGCA 1 cut(s) 1080
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 6 cut(s) 842, 930, 1085, 2068, 2210, 2493
NspV TTCGAA 1 cut(s) 1568
OliI CACNNNNGTG 1 cut(s) 2314
PaeI GCATGC 1 cut(s) 842
PagI TCATGA 1 cut(s) 726
PaqCI CACCTGC 1 cut(s) 1151
PceI AGGCCT 1 cut(s) 294
PciI ACATGT 2 cut(s) 2064, 2206
PciSI GCTCTTC 1 cut(s) 2394
PctI GAATGC 2 cut(s) 1127, 2298
PflMI CCANNNNNTGG 1 cut(s) 225
PinAI ACCGGT 2 cut(s) 504, 2165
PkrI GCNGC 4 cut(s) 588, 1546, 2136, 2386
PleI GAGTC 5 cut(s) 664, 740, 1061, 1799, 2183
PpsI GAGTC 5 cut(s) 664, 740, 1061, 1799, 2183
PscI ACATGT 2 cut(s) 2064, 2206
PsiI TTATAA 1 cut(s) 1605
Psp1406I AACGTT 1 cut(s) 178
Psp6I CCWGG 4 cut(s) 160, 934, 1665, 2407
PspEI GGTNACC 1 cut(s) 1163
PspGI CCWGG 4 cut(s) 160, 934, 1665, 2407
PspN4I GGNNCC 6 cut(s) 422, 509, 1062, 1101, 1597, 1724
PspPI GGNCC 6 cut(s) 624, 795, 878, 1100, 1383, 1595
PsrI GAACNNNNNNTAC 2 cut(s) 2153, 2185
PstI CTGCAG 2 cut(s) 1546, 2464
PstNI CAGNNNCTG 1 cut(s) 1487
PsuI RGATCY 3 cut(s) 629, 1510, 1887
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 8 cut(s) 599, 833, 925, 968, 1199, 2330, 2452, 2458
RsaNI GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2329, 2451, 2457
RseI CAYNNNNRTG 2 cut(s) 1015, 2314
SalI GTCGAC 1 cut(s) 2249
SapI GCTCTTC 1 cut(s) 2394
SaqAI TTAA 5 cut(s) 198, 501, 738, 1502, 1916
SatI GCNGC 4 cut(s) 587, 1545, 2135, 2385
Sau96I GGNCC 6 cut(s) 624, 795, 878, 1100, 1383, 1595
ScaI AGTACT 3 cut(s) 833, 2452, 2458
SchI GAGTC 5 cut(s) 664, 740, 1061, 1799, 2183
ScrFI CCNGG 7 cut(s) 162, 539, 540, 845, 936, 1667, 2409
SfaNI GCATC 6 cut(s) 271, 1019, 1474, 1758, 2371, 2455
SfcI CTRYAG 4 cut(s) 1371, 1542, 2116, 2460
SfuI TTCGAA 1 cut(s) 1568
SinI GGWCC 4 cut(s) 795, 878, 1100, 1383
SmaI CCCGGG 1 cut(s) 540
SmiMI CAYNNNNRTG 2 cut(s) 1015, 2314
SmlI CTYRAG 4 cut(s) 124, 757, 970, 1239
SmoI CTYRAG 4 cut(s) 124, 757, 970, 1239
SpeI ACTAGT 1 cut(s) 2453
SphI GCATGC 1 cut(s) 842
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 6 cut(s) 418, 584, 622, 908, 1169, 1587
SspI AATATT 2 cut(s) 1951, 2007
SspMI CTAG 9 cut(s) 146, 272, 561, 1473, 1620, 1881, 2001, 2138, 2454
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 7 cut(s) 160, 537, 538, 843, 934, 1665, 2407
StyI CCWWGG 3 cut(s) 369, 1095, 2238
TaaI ACNGT 5 cut(s) 605, 795, 893, 2120, 2295
TaiI ACGT 2 cut(s) 117, 181
TaqI TCGA 8 cut(s) 279, 789, 918, 1255, 1295, 1568, 1910, 2250
TatI WGTACW 5 cut(s) 597, 831, 923, 2450, 2456
Tru1I TTAA 5 cut(s) 198, 501, 738, 1502, 1916
Tru9I TTAA 5 cut(s) 198, 501, 738, 1502, 1916
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 8 cut(s) 700, 857, 893, 1047, 1163, 1189, 1234, 2067
TseI GCWGC 4 cut(s) 586, 1544, 2134, 2384
Tsp45I GTSAC 8 cut(s) 700, 857, 893, 1047, 1163, 1189, 1234, 2067
TspGWI ACGGA 4 cut(s) 712, 1003, 1041, 1375
TspMI CCCGGG 1 cut(s) 538
TspRI CASTG 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 225
VpaK11BI GGWCC 4 cut(s) 795, 878, 1100, 1383
XapI RAATTY 6 cut(s) 4, 166, 550, 1555, 1657, 2194
XceI RCATGY 6 cut(s) 842, 930, 1085, 2068, 2210, 2493
XcmI CCANNNNNNNNNTGG 1 cut(s) 1798
XmaI CCCGGG 1 cut(s) 538
XmiI GTMKAC 2 cut(s) 2115, 2250
XspI CTAG 9 cut(s) 146, 272, 561, 1473, 1620, 1881, 2001, 2138, 2454
ZrmI AGTACT 3 cut(s) 833, 2452, 2458
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.