RchiOBHm_Chr2g0119981

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000099
Physical Location & Seq
Forward (+)
32689231 .. 32692644
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49263

Sequence Viewer

Length: 2535 bp
ATGAATTCTATTCAATGGTCTATGATCACTGCATTGCTCATCTTCCTGCTTCCTTATCTACCCTCTTGCATTTCCATTGATTCCATTACTCCAAACCAAACCATCAAAGACGGCGACGTTTTAGTCTCAAGCAAGAAGCTCTTTGCGCTAGGGTTCTTCAGCCCTGGAAATTCTGAAAAACGTTATGTTGGAGTTTGGTTTGACAAAGTTCCAGAGCAAACCATTGTTTGGGTGGCAAATAGGGACAACCCTATCAATGATTCCTCTGGATTTCTAGTGATCGATGCAGATGGAGGTCTTGTCATATATGGAAAGGACAGAAAACTCCCTCTTTGGTCAGCTAATGTTACTCTCTCTTATCCAAAAAACTCTATGGCCAAGCTGCTGGATACAGGAAACTTTGTTTTGTTTGAGAATGGACGGAGCCTGTGGGAAGGGTTTGATTATCCCTCAAATACAATGCTTCCCTTTATGAAAATTGGGCTGAACCGGCGATCTGGGCTTAACCGGTTCCTAACTTCTTGGAAGTCCCAAGATGACCCGGGAACTGGGAATTTCTCAGCTAGGATTGATCCAGAAGGGTTTCCACAGATGTTTCTGTACAAAGGTCAAGCTCCCCGGTGGCGGACCGGATCTTGGATCGGGCACAGATGGAGTGGTGTCCCCATAATGGATGGCCTTAAGTTCGACTCAATCTTCGACGTTAGTTTTGTGAACAATCAAGATGAGCTATACGTCATGGATAGAATTAAGGACGACTCAATCTTCTCAAGGATGGTGCTTGTTGAATCGGGAATCCTCGAACGGTCCATGTGGCAAGATCAGCAACACCAATGGAGCAAGTATTGGTCTGGCCCGGTAGAGCTATGTGACAACTATGGAGAGTGTGGTCCAAATAGCAACTGTGACCCTTACGTATACAATGCTGGTATGTTCGAGTGTGCATGCTTACCAGGGTTTGAACCTAAGTTGAACCATTCATGGTACTTGAAAGATGGCTCGGGTGGGTGTGTGAGGAAAAAAGGAGCATCTGTGTGTAAAAACGGAGAAGGGTTCGTGATGGTGTCACCAGTAAAGGCACCAGACTCATCTACTGCGCATGTAAACTTGACCTTGGGTCCGAAAGAATGTAAAGAGGAATGCTTAAGGAATTGTTCTTGCATGGCATACTCGAGTGCAGGTGACCCGCAAGAATGGAATGGATGTGTGACATGGTACGGGGACTTGATGGATACAAGGACTTACACGAGTGTCACTCAAGATTTATATGTTCGAGTTGATTCAATTGAATTAGCTAAATACGCAAAGAAGTCGAATGGTTTTCTTAGCAAGAAGGGAAAGCTGGCAATTACGATAGCATCTATTTTAGTGTTCTTTGTTGTGATCTCTATCGCATATTGGTCCACAAAGATGAAGAGAAAAGGTACGGAAAGACAAAATCTATTTTCATTTGGTGTGATCACACCTTCAGTCTACTTGGAAGATTTTCCTAGTAGAACAGATCCTGATGATAGCAGATTAAAGTCGGATATACCTTTCTTTGATCTAAGCACCATATCTGCAGCCACAAACAATTTCTCTGATTCGAACAGGCTTGGAGAAGGCGGATTCGGGCCCGTTTATAAGGGAGTGCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTAAGTAAAAATTCTGGCCAGGGAAATGAAGAGTTCAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTCAGAATCTTAGGCTATTGCATTCAAGATGAAGAGAAGATGCTAATCTATGAATACCTGCCTAACAAAAGCCTGGACTCGTTCATATTCAATGAAATGAAAAGGACATCTCTGGATTGGACAAGACGCTTAGAGATTATCTTTGGCATTGCTAGAGGGATCTTGTATCTTCATCAAGATTCGAGATTAAGAATCATCCACAGAGACCTAAAGGCCAGCAATATTCTACTGGATTATGATATGAATCCAAAAATTGCAGATTTTGGTGTGGCTAGAATATTCAGAGCAAACCAAATTGCAGCAAGTACAAATCGGGTGGTTGGAACATATGGTTACATGTCACCGGAGTATGCGATGGAAGGACTGTTTTCAGTGAAGTCTAATGTATACAGTTTCGGTGTTCTACTGCTAGAAATCGTTACTGGCAGAAAGAACACTGGTTACTACTATGATAATCCTGACTCAAATTTGGTTGGACATGTTTGGGACTTGTGGAAAGAAGGTCGAGCCTTGGAAATCGTCGACACATCTTTGGGTGAATCCTACCCCATAAGTGAAGTTCTACGGTGCATTCAAATTGCGCTCTTGTGCGTGCAAGAGTATGCAAATGACCGGCCAACCATGTCAAAAGTTGTGTCTGTGTTAGGTAACGATGCAGCTCTTCCTTCACCAAGAAAACCTGGATTTTTGTTGAAGAGAAGTAACTATACCAGTGGAGACCCCAGTACTAGTATTGGAGATGCTAATTCCATAAATTATGTTACAAGCACTATAGTTGAAGCTCGATAA

Protein Analysis

844

Amino Acids

94.78

Weight (kDa)

6.74

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 176 4.9e-31 D-mannose binding lectin
S_locus_glycop PF00954 208 - 323 3.7e-24 S-locus glycoprotein domain
PAN_2 PF08276 346 - 412 3.6e-17 PAN-like domain
Pkinase PF00069 528 - 794 7.3e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 530 - 797 1.5e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1623
AarI CACCTGC 1 cut(s) 1169
AasI GACNNNNNNGTC 1 cut(s) 122
Acc16I TGCGCA 1 cut(s) 1098
Acc36I ACCTGC 2 cut(s) 1169, 1812
AccB1I GGYRCC 1 cut(s) 1078
AccB7I CCANNNNNTGG 1 cut(s) 228
AccI GTMKAC 4 cut(s) 918, 1473, 2133, 2268
AciI CCGC 3 cut(s) 625, 1187, 1605
AclI AACGTT 1 cut(s) 181
AclWI GGATC 5 cut(s) 566, 640, 647, 1496, 1913
AcoI YGGCCR 3 cut(s) 375, 1681, 2360
AcsI RAATTY 5 cut(s) 4, 169, 553, 1675, 2212
AcuI CTGAAG 2 cut(s) 142, 1452
AfaI GTAC 6 cut(s) 602, 986, 1217, 1426, 2053, 2473
AfiI CCNNNNNNNGG 5 cut(s) 228, 548, 624, 636, 670
AflII CTTAAG 2 cut(s) 680, 1144
AflIII ACRYGT 2 cut(s) 2082, 2224
AgeI ACCGGT 1 cut(s) 507
AhdI GACNNNNNGTC 1 cut(s) 1116
AhlI ACTAGT 1 cut(s) 2474
AjnI CCWGG 5 cut(s) 163, 952, 1683, 1818, 2425
Alw26I GTCTC 3 cut(s) 130, 1944, 2457
AlwI GGATC 5 cut(s) 566, 640, 647, 1496, 1913
AlwNI CAGNNNCTG 1 cut(s) 1505
Ama87I CYCGRG 3 cut(s) 541, 1000, 1171
AoxI GGCC 7 cut(s) 375, 676, 853, 1613, 1681, 1959, 2360
ApaI GGGCCC 1 cut(s) 1617
ApeKI GCWGC 4 cut(s) 382, 1562, 2045, 2402
ApoI RAATTY 5 cut(s) 4, 169, 553, 1675, 2212
ArsI GACNNNNNNTTYG 6 cut(s) 680, 692, 712, 724, 2366, 2398
AsiGI ACCGGT 1 cut(s) 507
Asp700I GAANNNNTTC 2 cut(s) 582, 1485
AspLEI GCGC 3 cut(s) 148, 1099, 2329
AspS9I GGNCC 8 cut(s) 627, 807, 854, 890, 1118, 1401, 1613, 1614
AsuC2I CCSGG 4 cut(s) 542, 543, 619, 857
AsuHPI GGTGA 5 cut(s) 1059, 1193, 2079, 2294, 2406
AsuII TTCGAA 1 cut(s) 1586
AvaI CYCGRG 3 cut(s) 541, 1000, 1171
AvaII GGWCC 5 cut(s) 627, 807, 890, 1118, 1401
BaeGI GKGCMC 2 cut(s) 648, 1617
BalI TGGCCA 2 cut(s) 377, 1683
BanI GGYRCC 1 cut(s) 1078
BanII GRGCYC 1 cut(s) 1617
BarI GAAGNNNNNNTAC 2 cut(s) 2437, 2469
BauI CACGAG 1 cut(s) 1246
BbvI GCAGC 4 cut(s) 369, 1574, 2057, 2414
BccI CCATC 9 cut(s) 110, 284, 645, 668, 769, 989, 1054, 1222, 2095
BceAI ACGGC 2 cut(s) 127, 1640
BcgI CGANNNNNNTGC 2 cut(s) 1293, 1327
BciT130I CCWGG 5 cut(s) 165, 954, 1685, 1820, 2427
BciVI GTATCC 2 cut(s) 382, 1225
BclI TGATCA 2 cut(s) 24, 1458
BcnI CCSGG 4 cut(s) 542, 543, 619, 857
BcoDI GTCTC 3 cut(s) 130, 1944, 2457
BcuI ACTAGT 1 cut(s) 2474
BfaI CTAG 9 cut(s) 149, 275, 564, 1491, 1638, 1899, 2019, 2156, 2475
BfmI CTRYAG 2 cut(s) 1560, 2517
BfrI CTTAAG 2 cut(s) 680, 1144
BfuAI ACCTGC 2 cut(s) 1169, 1812
BfuI GTATCC 2 cut(s) 382, 1225
BisI GCNGC 4 cut(s) 383, 1563, 2046, 2403
BlsI GCNGC 4 cut(s) 384, 1564, 2047, 2404
BmcAI AGTACT 1 cut(s) 2473
Bme1390I CCNGG 9 cut(s) 165, 542, 543, 619, 857, 954, 1685, 1820, 2427
Bme18I GGWCC 5 cut(s) 627, 807, 890, 1118, 1401
BmeRI GACNNNNNGTC 1 cut(s) 1116
BmeT110I CYCGRG 3 cut(s) 541, 1000, 1171
BmgT120I GGNCC 8 cut(s) 627, 807, 854, 890, 1118, 1401, 1613, 1614
BmiI GGNNCC 6 cut(s) 425, 512, 1080, 1119, 1615, 1742
BmrFI CCNGG 9 cut(s) 165, 542, 543, 619, 857, 954, 1685, 1820, 2427
BmrI ACTGGG 2 cut(s) 558, 2463
BmsI GCATC 6 cut(s) 274, 1037, 1367, 1776, 2389, 2476
BmuI ACTGGG 2 cut(s) 558, 2463
BplI GAGNNNNNCTC 2 cut(s) 1240, 1272
Bpu14I TTCGAA 1 cut(s) 1586
BpuEI CTTGAG 3 cut(s) 112, 754, 1242
BpuMI CCSGG 4 cut(s) 542, 543, 619, 857
Bsa29I ATCGAT 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 916
BsaBI GATNNNNATC 3 cut(s) 1388, 1790, 1989
BsaI GGTCTC 2 cut(s) 1944, 2457
BsaJI CCNNGG 7 cut(s) 163, 541, 617, 953, 1113, 1684, 2256
BsaWI WCCGGW 3 cut(s) 507, 629, 2089
BsaXI ACNNNNNCTCC 4 cut(s) 285, 315, 1017, 1047
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 548, 624, 636, 670
Bse118I RCCGGY 3 cut(s) 489, 507, 2358
Bse1I ACTGG 7 cut(s) 553, 1070, 1980, 2173, 2188, 2457, 2469
Bse3DI GCAATG 2 cut(s) 32, 1893
Bse8I GATNNNNATC 3 cut(s) 1388, 1790, 1989
BseBI CCWGG 5 cut(s) 165, 954, 1685, 1820, 2427
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 7 cut(s) 163, 541, 617, 953, 1113, 1684, 2256
BseGI GGATG 4 cut(s) 679, 780, 1208, 1941
BseJI GATNNNNATC 3 cut(s) 1388, 1790, 1989
BseLI CCNNNNNNNGG 5 cut(s) 228, 548, 624, 636, 670
BseMI GCAATG 2 cut(s) 32, 1893
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 7 cut(s) 553, 1070, 1980, 2173, 2188, 2457, 2469
BseSI GKGCMC 2 cut(s) 648, 1617
BseXI GCAGC 4 cut(s) 369, 1574, 2057, 2414
BsgI GTGCAG 1 cut(s) 1197
BshFI GGCC 7 cut(s) 377, 678, 855, 1615, 1683, 1961, 2362
BshNI GGYRCC 1 cut(s) 1078
BshTI ACCGGT 1 cut(s) 507
BshVI ATCGAT 1 cut(s) 282
BsiHKCI CYCGRG 3 cut(s) 541, 1000, 1171
BsiSI CCGG 8 cut(s) 490, 508, 542, 619, 630, 857, 2090, 2359
BslFI GGGAC 5 cut(s) 257, 514, 647, 1235, 2246
BslI CCNNNNNNNGG 5 cut(s) 228, 548, 624, 636, 670
BsmAI GTCTC 3 cut(s) 130, 1944, 2457
BsmFI GGGAC 5 cut(s) 257, 514, 647, 1235, 2246
BsmI GAATGC 3 cut(s) 1145, 1767, 2316
BsnI GGCC 7 cut(s) 377, 678, 855, 1615, 1683, 1961, 2362
Bso31I GGTCTC 2 cut(s) 1944, 2457
BsoBI CYCGRG 3 cut(s) 541, 1000, 1171
Bsp119I TTCGAA 1 cut(s) 1586
Bsp120I GGGCCC 1 cut(s) 1613
Bsp1286I GDGCHC 2 cut(s) 648, 1617
Bsp1407I TGTACA 1 cut(s) 600
BspACI CCGC 3 cut(s) 625, 1187, 1605
BspANI GGCC 7 cut(s) 377, 678, 855, 1615, 1683, 1961, 2362
BspCNI CTCAG 1 cut(s) 572
BspDI ATCGAT 1 cut(s) 282
BspLI GGNNCC 6 cut(s) 425, 512, 1080, 1119, 1615, 1742
BspMAI CTGCAG 1 cut(s) 1564
BspMI ACCTGC 2 cut(s) 1169, 1812
BspPI GGATC 5 cut(s) 566, 640, 647, 1496, 1913
BspQI GCTCTTC 1 cut(s) 2412
BspT104I TTCGAA 1 cut(s) 1586
BspT107I GGYRCC 1 cut(s) 1078
BspTI CTTAAG 2 cut(s) 680, 1144
BspTNI GGTCTC 2 cut(s) 1944, 2457
BsrDI GCAATG 2 cut(s) 32, 1893
BsrFI RCCGGY 3 cut(s) 489, 507, 2358
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 7 cut(s) 553, 1070, 1980, 2173, 2188, 2457, 2469
BssAI RCCGGY 3 cut(s) 489, 507, 2358
BssECI CCNNGG 7 cut(s) 163, 541, 617, 953, 1113, 1684, 2256
BssNAI GTATAC 2 cut(s) 919, 2134
BssSI CACGAG 1 cut(s) 1246
BssT1I CCWWGG 2 cut(s) 1113, 2256
Bst1107I GTATAC 2 cut(s) 919, 2134
Bst2BI CACGAG 1 cut(s) 1246
Bst2UI CCWGG 5 cut(s) 165, 954, 1685, 1820, 2427
Bst4CI ACNGT 5 cut(s) 807, 905, 2112, 2138, 2313
Bst6I CTCTTC 5 cut(s) 1409, 1689, 1773, 2412, 2435
BstAFI CTTAAG 2 cut(s) 680, 1144
BstAUI TGTACA 1 cut(s) 600
BstBAI YACGTR 1 cut(s) 916
BstBI TTCGAA 1 cut(s) 1586
BstC8I GCNNGC 4 cut(s) 946, 1344, 1963, 2339
BstDEI CTNAG 7 cut(s) 559, 966, 1325, 1547, 1667, 1756, 1876
BstEII GGTNACC 1 cut(s) 1181
BstF5I GGATG 4 cut(s) 679, 780, 1208, 1941
BstHHI GCGC 3 cut(s) 148, 1099, 2329
BstMAI GTCTC 3 cut(s) 130, 1944, 2457
BstMWI GCNNNNNNNGC 5 cut(s) 145, 490, 499, 823, 1301
BstNI CCWGG 5 cut(s) 165, 954, 1685, 1820, 2427
BstNSI RCATGY 4 cut(s) 948, 1103, 2086, 2228
BstPI GGTNACC 1 cut(s) 1181
BstSCI CCNGG 9 cut(s) 163, 540, 541, 617, 855, 952, 1683, 1818, 2425
BstSFI CTRYAG 2 cut(s) 1560, 2517
BstSLI GKGCMC 2 cut(s) 648, 1617
BstSNI TACGTA 1 cut(s) 916
BstV1I GCAGC 4 cut(s) 369, 1574, 2057, 2414
BstX2I RGATCY 3 cut(s) 632, 1501, 1905
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 3 cut(s) 632, 1501, 1905
BstZ17I GTATAC 2 cut(s) 919, 2134
Bsu15I ATCGAT 1 cut(s) 282
BsuI GTATCC 2 cut(s) 382, 1225
BsuRI GGCC 7 cut(s) 377, 678, 855, 1615, 1683, 1961, 2362
BsuTUI ATCGAT 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 2114
BtsCI GGATG 4 cut(s) 679, 780, 1208, 1941
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 4 cut(s) 27, 2124, 2181, 2464
BveI ACCTGC 2 cut(s) 1169, 1812
Cac8I GCNNGC 4 cut(s) 946, 1344, 1963, 2339
CaiI CAGNNNCTG 1 cut(s) 1505
CfoI GCGC 3 cut(s) 148, 1099, 2329
Cfr10I RCCGGY 3 cut(s) 489, 507, 2358
Cfr13I GGNCC 8 cut(s) 627, 807, 854, 890, 1118, 1401, 1613, 1614
Cfr9I CCCGGG 1 cut(s) 541
ClaI ATCGAT 1 cut(s) 282
CpoI CGGWCCG 1 cut(s) 627
CseI GACGC 1 cut(s) 1881
Csp6I GTAC 6 cut(s) 601, 985, 1216, 1425, 2052, 2472
CspAI ACCGGT 1 cut(s) 507
CspCI CAANNNNNGTGG 2 cut(s) 2043, 2078
CspI CGGWCCG 1 cut(s) 627
CviQI GTAC 6 cut(s) 601, 985, 1216, 1425, 2052, 2472
DdeI CTNAG 7 cut(s) 559, 966, 1325, 1547, 1667, 1756, 1876
DrdI GACNNNNNNGTC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 1116
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 3 cut(s) 375, 1681, 2360
Eam1104I CTCTTC 5 cut(s) 1409, 1689, 1773, 2412, 2435
Eam1105I GACNNNNNGTC 1 cut(s) 1116
EarI CTCTTC 5 cut(s) 1409, 1689, 1773, 2412, 2435
EciI GGCGGA 2 cut(s) 640, 1620
Eco105I TACGTA 1 cut(s) 916
Eco130I CCWWGG 2 cut(s) 1113, 2256
Eco24I GRGCYC 1 cut(s) 1617
Eco31I GGTCTC 2 cut(s) 1944, 2457
Eco47I GGWCC 5 cut(s) 627, 807, 890, 1118, 1401
Eco57I CTGAAG 2 cut(s) 142, 1452
Eco88I CYCGRG 3 cut(s) 541, 1000, 1171
Eco91I GGTNACC 1 cut(s) 1181
EcoO65I GGTNACC 1 cut(s) 1181
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 5 cut(s) 163, 952, 1683, 1818, 2425
EcoT14I CCWWGG 2 cut(s) 1113, 2256
EcoT38I GRGCYC 1 cut(s) 1617
ErhI CCWWGG 2 cut(s) 1113, 2256
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FaqI GGGAC 5 cut(s) 257, 514, 647, 1235, 2246
FauI CCCGC 1 cut(s) 1194
FauNDI CATATG 1 cut(s) 2074
FbaI TGATCA 2 cut(s) 24, 1458
FblI GTMKAC 4 cut(s) 918, 1473, 2133, 2268
Fnu4HI GCNGC 4 cut(s) 383, 1563, 2046, 2403
FokI GGATG 4 cut(s) 686, 787, 1215, 1928
FriOI GRGCYC 1 cut(s) 1617
Fsp4HI GCNGC 4 cut(s) 383, 1563, 2046, 2403
FspBI CTAG 9 cut(s) 149, 275, 564, 1491, 1638, 1899, 2019, 2156, 2475
FspI TGCGCA 1 cut(s) 1098
GlaI GCGC 3 cut(s) 147, 1098, 2328
GluI GCNGC 4 cut(s) 383, 1563, 2046, 2403
HaeIII GGCC 7 cut(s) 377, 678, 855, 1615, 1683, 1961, 2362
HapII CCGG 8 cut(s) 490, 508, 542, 619, 630, 857, 2090, 2359
HgaI GACGC 1 cut(s) 1881
HhaI GCGC 3 cut(s) 148, 1099, 2329
Hin6I GCGC 3 cut(s) 146, 1097, 2327
HinP1I GCGC 3 cut(s) 146, 1097, 2327
HincII GTYRAC 1 cut(s) 2269
HindII GTYRAC 1 cut(s) 2269
HpaII CCGG 8 cut(s) 490, 508, 542, 619, 630, 857, 2090, 2359
HphI GGTGA 5 cut(s) 1059, 1193, 2079, 2294, 2406
Hpy166II GTNNAC 7 cut(s) 715, 919, 1105, 1404, 1474, 2134, 2269
Hpy188I TCNGA 6 cut(s) 175, 1122, 1528, 1582, 1751, 2030
Hpy8I GTNNAC 7 cut(s) 715, 919, 1105, 1404, 1474, 2134, 2269
Hpy99I CGWCG 3 cut(s) 119, 704, 2270
HpyAV CCTTC 9 cut(s) 428, 572, 1043, 1327, 1476, 1595, 2099, 2240, 2421
HpyCH4III ACNGT 5 cut(s) 807, 905, 2112, 2138, 2313
HpyCH4IV ACGT 5 cut(s) 117, 181, 702, 735, 915
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 490, 499, 823, 1301
HpyF3I CTNAG 7 cut(s) 559, 966, 1325, 1547, 1667, 1756, 1876
HpySE526I ACGT 5 cut(s) 117, 181, 702, 735, 915
HspAI GCGC 3 cut(s) 146, 1097, 2327
Ksp22I TGATCA 2 cut(s) 24, 1458
LguI GCTCTTC 1 cut(s) 2412
LmnI GCTCC 4 cut(s) 423, 619, 837, 1025
Lsp1109I GCAGC 4 cut(s) 369, 1574, 2057, 2414
LweI GCATC 6 cut(s) 274, 1037, 1367, 1776, 2389, 2476
MaeI CTAG 9 cut(s) 149, 275, 564, 1491, 1638, 1899, 2019, 2156, 2475
MaeII ACGT 5 cut(s) 117, 181, 702, 735, 915
MfeI CAATTG 1 cut(s) 1284
MflI RGATCY 3 cut(s) 632, 1501, 1905
MhlI GDGCHC 2 cut(s) 648, 1617
MlsI TGGCCA 2 cut(s) 377, 1683
MluNI TGGCCA 2 cut(s) 377, 1683
MlyI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
MmeI TCCRAC 4 cut(s) 169, 1506, 2047, 2200
MnlI CCTC 9 cut(s) 73, 274, 287, 339, 460, 809, 1008, 1129, 1895
Mox20I TGGCCA 2 cut(s) 377, 1683
MroXI GAANNNNTTC 2 cut(s) 582, 1485
MscI TGGCCA 2 cut(s) 377, 1683
MseI TTAA 6 cut(s) 504, 681, 750, 1145, 1520, 1934
MslI CAYNNNNRTG 2 cut(s) 1033, 1409
Msp20I TGGCCA 2 cut(s) 377, 1683
MspCI CTTAAG 2 cut(s) 680, 1144
MspI CCGG 8 cut(s) 490, 508, 542, 619, 630, 857, 2090, 2359
MspR9I CCNGG 9 cut(s) 165, 542, 543, 619, 857, 954, 1685, 1820, 2427
MunI CAATTG 1 cut(s) 1284
Mva1269I GAATGC 3 cut(s) 1145, 1767, 2316
MvaI CCWGG 5 cut(s) 165, 954, 1685, 1820, 2427
MwoI GCNNNNNNNGC 5 cut(s) 145, 490, 499, 823, 1301
NciI CCSGG 4 cut(s) 542, 543, 619, 857
NdeI CATATG 1 cut(s) 2074
NlaIV GGNNCC 6 cut(s) 425, 512, 1080, 1119, 1615, 1742
NmuCI GTSAC 7 cut(s) 869, 905, 1065, 1181, 1207, 1252, 2085
NsbI TGCGCA 1 cut(s) 1098
NspI RCATGY 4 cut(s) 948, 1103, 2086, 2228
NspV TTCGAA 1 cut(s) 1586
PaeI GCATGC 1 cut(s) 948
PaeR7I CTCGAG 1 cut(s) 1171
PaqCI CACCTGC 1 cut(s) 1169
PciI ACATGT 2 cut(s) 2082, 2224
PciSI GCTCTTC 1 cut(s) 2412
PctI GAATGC 3 cut(s) 1145, 1767, 2316
PdmI GAANNNNTTC 2 cut(s) 582, 1485
PflMI CCANNNNNTGG 1 cut(s) 228
PinAI ACCGGT 1 cut(s) 507
PkrI GCNGC 4 cut(s) 384, 1564, 2047, 2404
PleI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
PpsI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
Ppu21I YACGTR 1 cut(s) 916
PscI ACATGT 2 cut(s) 2082, 2224
PsiI TTATAA 1 cut(s) 1623
Psp1406I AACGTT 1 cut(s) 181
Psp6I CCWGG 5 cut(s) 163, 952, 1683, 1818, 2425
PspEI GGTNACC 1 cut(s) 1181
PspGI CCWGG 5 cut(s) 163, 952, 1683, 1818, 2425
PspN4I GGNNCC 6 cut(s) 425, 512, 1080, 1119, 1615, 1742
PspOMI GGGCCC 1 cut(s) 1613
PspPI GGNCC 8 cut(s) 627, 807, 854, 890, 1118, 1401, 1613, 1614
PspXI VCTCGAGB 1 cut(s) 1171
PsrI GAACNNNNNNTAC 2 cut(s) 2171, 2203
PstI CTGCAG 1 cut(s) 1564
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 3 cut(s) 632, 1501, 1905
RsaI GTAC 6 cut(s) 602, 986, 1217, 1426, 2053, 2473
RsaNI GTAC 6 cut(s) 601, 985, 1216, 1425, 2052, 2472
RseI CAYNNNNRTG 2 cut(s) 1033, 1409
Rsr2I CGGWCCG 1 cut(s) 627
RsrII CGGWCCG 1 cut(s) 627
SalI GTCGAC 1 cut(s) 2267
SapI GCTCTTC 1 cut(s) 2412
SaqAI TTAA 6 cut(s) 504, 681, 750, 1145, 1520, 1934
SatI GCNGC 4 cut(s) 383, 1563, 2046, 2403
Sau96I GGNCC 8 cut(s) 627, 807, 854, 890, 1118, 1401, 1613, 1614
ScaI AGTACT 1 cut(s) 2473
SchI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
ScrFI CCNGG 9 cut(s) 165, 542, 543, 619, 857, 954, 1685, 1820, 2427
SduI GDGCHC 2 cut(s) 648, 1617
SfaNI GCATC 6 cut(s) 274, 1037, 1367, 1776, 2389, 2476
SfcI CTRYAG 2 cut(s) 1560, 2517
Sfr274I CTCGAG 1 cut(s) 1171
SfuI TTCGAA 1 cut(s) 1586
SinI GGWCC 5 cut(s) 627, 807, 890, 1118, 1401
SlaI CTCGAG 1 cut(s) 1171
SmaI CCCGGG 1 cut(s) 543
SmiMI CAYNNNNRTG 2 cut(s) 1033, 1409
SmlI CTYRAG 6 cut(s) 127, 680, 769, 1144, 1171, 1257
SmoI CTYRAG 6 cut(s) 127, 680, 769, 1144, 1171, 1257
SnaBI TACGTA 1 cut(s) 916
SpeI ACTAGT 1 cut(s) 2474
SphI GCATGC 1 cut(s) 948
SsiI CCGC 3 cut(s) 625, 1187, 1605
SspI AATATT 2 cut(s) 1969, 2025
SspMI CTAG 9 cut(s) 149, 275, 564, 1491, 1638, 1899, 2019, 2156, 2475
StyD4I CCNGG 9 cut(s) 163, 540, 541, 617, 855, 952, 1683, 1818, 2425
StyI CCWWGG 2 cut(s) 1113, 2256
TaaI ACNGT 5 cut(s) 807, 905, 2112, 2138, 2313
TaiI ACGT 5 cut(s) 120, 184, 705, 738, 918
TatI WGTACW 3 cut(s) 600, 2051, 2471
Tru1I TTAA 6 cut(s) 504, 681, 750, 1145, 1520, 1934
Tru9I TTAA 6 cut(s) 504, 681, 750, 1145, 1520, 1934
TscAI CASTG 4 cut(s) 34, 2124, 2188, 2464
TseFI GTSAC 7 cut(s) 869, 905, 1065, 1181, 1207, 1252, 2085
TseI GCWGC 4 cut(s) 382, 1562, 2045, 2402
Tsp45I GTSAC 7 cut(s) 869, 905, 1065, 1181, 1207, 1252, 2085
TspGWI ACGGA 3 cut(s) 436, 1059, 1442
TspMI CCCGGG 1 cut(s) 541
TspRI CASTG 4 cut(s) 34, 2124, 2188, 2464
Van91I CCANNNNNTGG 1 cut(s) 228
Vha464I CTTAAG 2 cut(s) 680, 1144
VpaK11BI GGWCC 5 cut(s) 627, 807, 890, 1118, 1401
XapI RAATTY 5 cut(s) 4, 169, 553, 1675, 2212
XceI RCATGY 4 cut(s) 948, 1103, 2086, 2228
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 1171
XmaI CCCGGG 1 cut(s) 541
XmiI GTMKAC 4 cut(s) 918, 1473, 2133, 2268
XmnI GAANNNNTTC 2 cut(s) 582, 1485
XspI CTAG 9 cut(s) 149, 275, 564, 1491, 1638, 1899, 2019, 2156, 2475
ZrmI AGTACT 1 cut(s) 2473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.