RLG00000031275

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000099
Physical Location & Seq
Reverse (-)
3628458 .. 3636214
7757 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031275

Sequence Viewer

Length: 4230 bp
ATGATTCCTCTGGAGTGCTATCGATCAGTGGTGATGGAGGCCTTGTCATACACGGAAAGGACCAAAAAGTCAAAAGATGACCCGGGAACCGGGAGCTGTACATATGGGATAGACCCGAGGGGGTCTCCGCAGTTGTTTCTGAAAAAGGGGGGAGGCCCTTTGTGGCGGAGCGGCCCTTGGATCGGGGACAGATGGAGCGGTGAACCCCAACTAACCAGTACCGTTGGACTCTTCAATTTAAGTTTTGTCAACAATGAAGACGAGATATCCTACAAGCGTGTTGTTGCAAATGACTCATTGTTCACAAGGGGGGTGCTAGATGAATCAGGAATATATCAACGGCTCATGTGGAATGATAAGGAAAACAAATGGATTGAATTGGTCTCCGACCCGAATGAGTGGTGTGATAACTATGGACTCTGTGGTCCAAATAGTAACTGTGACCCATACAATGACTATAAGTTTGCTTGCACTTGCCTACCCGGCTTCGAACCCAAATCGCCTCATGACTGGTATTTGAGAATTGGCGTGGGTGGTTGCATTAGGAAAGCAGGAGTGTCTGTGTGTCGGAATGGAGAAGGGTTCGTGCCGGTGGAGCGTGTAAAGGTACCGGACTCATCTACGGCAGTACGTGTGAATATGAGTTTGAGTTTGGAAGCTTGTAAGCTAGAGTGCTTGATGGATTGTTCTTGCACGGCTTACACGAGTGCAGATGAGCGGGGAGGTGGAAATGGGTGCTTGACATGGCACGGGGACTTGATGGACACGAGGACTTACCCCAAAGTTGGTCAAGATTTATATGTTCGAGTTGATGCAACTTTTCTGACTCAATATGCGAAATCAAATGGTTCGCTTAACAAGAAGGCGACGCCGGCAATTTCAAATGGTTCTCTTAGCAAGAAGGCGACGCTAGCAATTTCACTAGGATCTGTTACAGTTTTCCTTCTCTTACTTACCCTTTATTGGTTGGGCATACTTTACAATGGAAAGGAAATAGCAGTAAAAAGACTATCCAAGTTTTCTGGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTGATTGCAAAACTCCAACACAGAAACCTTGTCAGGATTTTAGGTTGTTGTGTTGAAGATGAAGAGAAGATTCTACTCTATGAATACTTGCCAAACATAAGTTTGGACTCTTTCATCTTTAATGAAACAAAGAGAGCACTTTTAAATTGGACAAAACGTTTCGAGATTATCTTTGGGATTGCTAGAGGGGTATTATATCTTCATGAAGATTCGAGATTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTCCTATTGGATAATTCTTTGAACCCAAAAATTGCAGATTTTGGTATGGCTAGAATATTTAAAGGGGAGCAAAGTGAAGCGAATACCAATCGTGTGGTTGGAACATATGGTTATATGTCCCCAGAATATGCAATGGAAGGACTGTTTTCAATAAAGTCTGATGTATATAGTTTTGGTGTTTTACTGCTAGAAATCATCACTGGCAAAAAGAATGTTGGCTATTACCATGAGGAGTATCCTCATTCAAATTTGGTTGGACATGTTTGGGACTTGTGGAAAGAAGGCAGAGCTGTGGAAATCTTTGATTCATCTATAGATGAATACCTTGTTAGTGAAGTTATAAGGTGCATTCAAATTGCGCTCTTGTGTGTTCAAGAATTTGCAACAGACCGGCCAACTATGTCAGCAGTTGTTTCCATGTTAGGTAATGATGCAGCTCTTCCTTCACCAAGACGACCCGCATTTTTACTAAAGAGAATGACTCCTAGTGGAGAACCATCCAGCGGTGAAGGAGCTAATTCAGAGCAGAATTGTAAGCCTGAATGTCATATTTTCGTTCGTCAAGAGAAGCCAACTTTAATTTCCTTTGAAATTCCTGTTCACAATGCACATTTGTCAGGTGTAAAAAGATGCACCTTGCATGAGGATCAAATCATTTCGGCTTTCACAAGGCCTTTTTTCTTCACATGCAAAATGAGCCCAGGAAATTCTGGTAAGCGTTATGTTGGAGTATGGTATAACCAAGTTCCTAACAAAACCATCGTTTGGGTTGCAAACAGGGACAATCCAGTCAATGATACCTCCGGATTCCTAGCAATTCAAGGAAATGGAGGCCTTGTCATCTACGGAAATGAACGAAATATCCCACTTTGGTCAGCTAATGTCAGTCTCTCTTCGCCAAACAGTTCCATTGCTAAGCTTCGGGATACAGGAAATCTTGTGTTGCTTGAGAATGGGGATAGTCAAAGGGTGGTGTGGCAAGGCTTTGATTATCCCACAGATACAATGCTTCCATCTATGAAACTTGGGGTGGACCGACGGTCTGGGTTGAACCGGTCCCTCACATCTTGGAAGTCCCAAACTGACCCGGGAAAAGGGAACTGCTCATATTGGGTTGATCCAGGTGGGTTACAGATGATTCTTTACAAGGATGGAGCTCCAAGGTGGCGGACCGAAATTGGATCTTGGACTGGCCAGAGATGGGGTGCTGTAACAACTTTTGTGAGCAATGAAGATGAGGTCTCGGTGGTGACTATTCCTACCACCAATCAGTCAACATTCCCCAGGACAGTCCTAGATGAATCGGGAATTCTTGGACGGTTCATGTGGGACAATAGGCTACATCAATGGACTGACTATTGGTCTGCCCCAAAAGAGCGTTGTGATTTCTATGGACAATGTGGTCCAAATGGTAACTGTGATCCATACACTGTTGACACCACACTGATTACAATCGGTTGCAGCTGCTTGCCCGGGTATGAACCCAAGTCTCCGGAAGATTGGTATATGAGAGATGGGTCAGGTGGCTGTGTAAAGGAAACAGGAATATCCATGTGCAGGAGTGGGGAAGGGTTCGTGAAGGTGAAAAATGTAAAACTACCGGATTCGTCTCTGGCAAGTGTAGACATGAGTTTAAGTTTGAAAGAGTGTGAGCAAAAGTGCTTGAAAAATTGTTCTTGCACTGCATACTCAAGTGCCAATGAGAGCAGGAGAGAGGTTGGATGTGTGACATGGTATGGGGACTTGATGGACACATCAACTAATCCAGACGTCGGGCAAGATTTATATGTCCGTGTTGATGCAGCGGTCTTAGCTGAATATGCTAAGAAGTCAAATGCTCTAAGCAAGAAGGGAAAGCTAGCAATTTCATTAGCCTCATCAGCTGTTCTCGCTTTACTTTTACTAGTTTCCCTTTTGTATTGGTTCATAAGGAGGAAGAGAAATGCTAAGCAAACACAAGAGAAATATATATTCCGAGTTAGCATGGAAGACTCCACTGGAGATCTTAATGATGAAAATACGATGAACTCAGACGGCATCCTATGTAATGGAACGGAAATAGCAGTTAAAAGGCTGTCGAAGCATTCTGGTCAAGGTGTCAAAGAGTTTAAGAATGAAGTTGTGCTGATTGCAAAACTCCAACACAGGAACCTTGTAAGGATTCTAGGTTATTGTGTTCAAGATGAAGAAAAGATGCTCATCTATGAATACGTGCCAAACAGAAGTTTGGACTTTTTCATTTTTGATGAAACAAAAAGAGCACTTTTAGATTGGACAAAACGCTTTGGGATCATTTGTGGAATTGCTAGAGGGATCTTATACCTTCATCAAGATTCAAGATTAAGAATCATCCATAGAGATCTAAAGGCGAGCAATATTCTACTTGATGGTTCAATGAATCCCAAAATTGCAGATTTTGGAATGGCTAGAATATTTAGTGGAGATCAAAGTGAAGCAAGTACAAGTCGTGTTGTCGGAACATATGGTTATATGTCACCGGAATATGCAATGGAAGGACTGTTTTCAGTAAAATCTGATGTGTATAGTTTTGGCATTTTACTACTCGAAATCATTGCAGGCAAGAGGAATGCTGGTTATTACCATGAGAAGTATCCAGATTCAAATTTGGTTGGCCATGTTTGGGAGTTGTGGAAAGAAGGTAAAGCCTTGGAAATAGTCGATTCCTGTCTAGGCGAAGCATATCCTGTCAATGGGGTTCTCAGATGCATCCAAATTGCGCTCTTGTGCGTGCAAGAACTTGCAACGGACCGACCAACCATGCCAGCTGTTGTTTTCATGTTAGGTAATGATGCTGCTCTTCCTTCAGCCGGACGACCTGCATTTTTAGTGAAGAGAATCTATTCCAATGGAGAGCTATCTGTCAATGATATCACATGTACAATGGTAGAAGCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

1410

Amino Acids

157.31

Weight (kDa)

6.5

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 55 - 167 1.7e-24 S-locus glycoprotein domain
PAN_2 PF08276 189 - 256 2.6e-19 PAN-like domain
Pkinase PF00069 328 - 515 4.7e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 329 - 577 1.8e-40 Protein tyrosine and serine/threonine kinase
B_lectin PF01453 690 - 795 4.5e-35 D-mannose binding lectin
S_locus_glycop PF00954 841 - 934 7.2e-17 S-locus glycoprotein domain
PAN_2 PF08276 956 - 1022 3.1e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 1121 - 1368 3.4e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1121 - 1299 5.1e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1652
AasI GACNNNNNNGTC 4 cut(s) 67, 423, 2099, 2712
AatII GACGTC 1 cut(s) 3084
Acc36I ACCTGC 1 cut(s) 4156
Acc65I GGTACC 1 cut(s) 607
AccB1I GGYRCC 1 cut(s) 607
AccB7I CCANNNNNTGG 1 cut(s) 2076
AccBSI CCGCTC 3 cut(s) 171, 198, 718
AccI GTMKAC 1 cut(s) 2934
AccIII TCCGGA 2 cut(s) 2114, 2803
AciI CCGC 9 cut(s) 128, 166, 171, 198, 718, 1770, 1815, 2479, 3116
AclI AACGTT 1 cut(s) 1216
AclWI GGATC 8 cut(s) 188, 934, 1965, 2423, 2500, 2726, 3608, 3632
AcoI YGGCCR 4 cut(s) 1024, 1703, 2503, 3943
AcsI RAATTY 6 cut(s) 1558, 1688, 1902, 2017, 2619, 3934
AcuI CTGAAG 1 cut(s) 4119
AcyI GRCGYC 2 cut(s) 869, 3081
AfaI GTAC 6 cut(s) 100, 220, 609, 630, 3772, 4210
AflIII ACRYGT 3 cut(s) 631, 1570, 4205
AgeI ACCGGT 1 cut(s) 2364
AhdI GACNNNNNGTC 2 cut(s) 2350, 2671
AhlI ACTAGT 1 cut(s) 3214
AjnI CCWGG 3 cut(s) 2011, 2431, 2594
AjuI GAANNNNNNNTTGG 2 cut(s) 1876, 1908
AloI GAACNNNNNNTCC 2 cut(s) 2157, 2189
Alw21I GWGCWC 3 cut(s) 1198, 2470, 3574
Alw26I GTCTC 6 cut(s) 129, 388, 2204, 2557, 2805, 2925
AlwI GGATC 8 cut(s) 188, 934, 1965, 2423, 2500, 2726, 3608, 3632
Ama87I CYCGRG 4 cut(s) 82, 115, 2398, 2783
Aor13HI TCCGGA 2 cut(s) 2114, 2803
ApeKI GCWGC 5 cut(s) 1745, 2772, 2775, 3113, 4124
ApoI RAATTY 6 cut(s) 1558, 1688, 1902, 2017, 2619, 3934
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AsiGI ACCGGT 1 cut(s) 2364
Asp700I GAANNNNTTC 2 cut(s) 1056, 4171
Asp718I GGTACC 1 cut(s) 607
AspLEI GCGC 2 cut(s) 1672, 4051
AspS9I GGNCC 9 cut(s) 60, 155, 173, 425, 2344, 2367, 2481, 2714, 4078
AsuC2I CCSGG 8 cut(s) 83, 84, 91, 483, 2399, 2400, 2784, 2785
AsuHPI GGTGA 7 cut(s) 43, 212, 1749, 1829, 2572, 2905, 3798
AsuII TTCGAA 1 cut(s) 489
AsuNHI GCTAGC 2 cut(s) 910, 3169
AvaI CYCGRG 4 cut(s) 82, 115, 2398, 2783
AvaII GGWCC 7 cut(s) 60, 425, 2344, 2367, 2481, 2714, 4078
BalI TGGCCA 3 cut(s) 1026, 2505, 3945
BanI GGYRCC 1 cut(s) 607
BanII GRGCYC 2 cut(s) 2012, 2470
BarI GAAGNNNNNNTAC 2 cut(s) 1242, 1274
BauI CACGAG 2 cut(s) 703, 766
BbsI GAAGAC 2 cut(s) 264, 3306
Bbv12I GWGCWC 3 cut(s) 1198, 2470, 3574
BbvI GCAGC 5 cut(s) 1757, 2762, 2784, 3125, 4111
BceAI ACGGC 4 cut(s) 356, 639, 711, 3361
BcgI CGANNNNNNTGC 2 cut(s) 3866, 3900
BciT130I CCWGG 3 cut(s) 2013, 2433, 2596
BciVI GTATCC 3 cut(s) 1557, 2230, 3933
BcnI CCSGG 8 cut(s) 83, 84, 91, 483, 2399, 2400, 2784, 2785
BcoDI GTCTC 6 cut(s) 129, 388, 2204, 2557, 2805, 2925
BcuI ACTAGT 1 cut(s) 3214
BfmI CTRYAG 1 cut(s) 1623
BfuAI ACCTGC 1 cut(s) 4156
BfuI GTATCC 3 cut(s) 1557, 2230, 3933
BglI GCCNNNNNGGC 1 cut(s) 483
BglII AGATCT 3 cut(s) 1294, 3313, 3670
BisI GCNGC 6 cut(s) 172, 1746, 2773, 2776, 3114, 4125
BlpI GCTNAGC 2 cut(s) 2226, 3258
BlsI GCNGC 6 cut(s) 173, 1747, 2774, 2777, 3115, 4126
Bme18I GGWCC 7 cut(s) 60, 425, 2344, 2367, 2481, 2714, 4078
BmeRI GACNNNNNGTC 2 cut(s) 2350, 2671
BmeT110I CYCGRG 4 cut(s) 82, 115, 2398, 2783
BmgT120I GGNCC 9 cut(s) 60, 155, 173, 425, 2344, 2367, 2481, 2714, 4078
BmiI GGNNCC 4 cut(s) 88, 609, 2369, 3461
BmsI GCATC 9 cut(s) 802, 1732, 1931, 3100, 3357, 3495, 4025, 4047, 4111
BmtI GCTAGC 2 cut(s) 914, 3173
BpiI GAAGAC 2 cut(s) 264, 3306
BplI GAGNNNNNCTC 4 cut(s) 109, 141, 1777, 1809
BpmI CTGGAG 2 cut(s) 32, 3330
Bpu1102I GCTNAGC 2 cut(s) 2226, 3258
Bpu14I TTCGAA 1 cut(s) 489
BpuEI CTTGAG 2 cut(s) 2279, 2986
BpuMI CCSGG 8 cut(s) 83, 84, 91, 483, 2399, 2400, 2784, 2785
Bsa29I ATCGAT 1 cut(s) 22
BsaAI YACGTR 2 cut(s) 632, 3523
BsaBI GATNNNNATC 2 cut(s) 2762, 3509
BsaHI GRCGYC 2 cut(s) 869, 3081
BsaI GGTCTC 3 cut(s) 129, 388, 2557
BsaWI WCCGGW 6 cut(s) 610, 2114, 2364, 2803, 2911, 3808
BsaXI ACNNNNNCTCC 6 cut(s) 29, 59, 546, 576, 2133, 2163
Bse118I RCCGGY 4 cut(s) 589, 871, 1701, 2364
Bse1I ACTGG 6 cut(s) 216, 515, 1516, 2099, 2506, 3313
Bse3DI GCAATG 6 cut(s) 1315, 1449, 2220, 2545, 3825, 3882
Bse8I GATNNNNATC 2 cut(s) 2762, 3509
BseAI TCCGGA 2 cut(s) 2114, 2803
BseBI CCWGG 3 cut(s) 2013, 2433, 2596
BseCI ATCGAT 1 cut(s) 22
BseGI GGATG 6 cut(s) 1808, 2467, 3038, 3348, 3660, 4038
BseJI GATNNNNATC 2 cut(s) 2762, 3509
BseMI GCAATG 6 cut(s) 1315, 1449, 2220, 2545, 3825, 3882
BseMII CTCAG 2 cut(s) 3354, 4045
BseNI ACTGG 6 cut(s) 216, 515, 1516, 2099, 2506, 3313
BseRI GAGGAG 1 cut(s) 1556
BseXI GCAGC 5 cut(s) 1757, 2762, 2784, 3125, 4111
BsgI GTGCAG 2 cut(s) 729, 2887
BshNI GGYRCC 1 cut(s) 607
BshTI ACCGGT 1 cut(s) 2364
BshVI ATCGAT 1 cut(s) 22
BsiHKAI GWGCWC 3 cut(s) 1198, 2470, 3574
BsiHKCI CYCGRG 4 cut(s) 82, 115, 2398, 2783
BslFI GGGAC 9 cut(s) 200, 767, 1414, 1592, 2105, 2353, 2371, 2654, 3065
BsmAI GTCTC 6 cut(s) 129, 388, 2204, 2557, 2805, 2925
BsmBI CGTCTC 1 cut(s) 2925
BsmFI GGGAC 9 cut(s) 200, 767, 1414, 1592, 2105, 2353, 2371, 2654, 3065
BsmI GAATGC 3 cut(s) 1659, 3394, 3904
Bso31I GGTCTC 3 cut(s) 129, 388, 2557
BsoBI CYCGRG 4 cut(s) 82, 115, 2398, 2783
Bsp119I TTCGAA 1 cut(s) 489
Bsp1286I GDGCHC 4 cut(s) 1198, 2012, 2470, 3574
Bsp13I TCCGGA 2 cut(s) 2114, 2803
Bsp1407I TGTACA 2 cut(s) 98, 4208
Bsp1720I GCTNAGC 2 cut(s) 2226, 3258
BspACI CCGC 9 cut(s) 128, 166, 171, 198, 718, 1770, 1815, 2479, 3116
BspCNI CTCAG 2 cut(s) 3353, 4044
BspDI ATCGAT 1 cut(s) 22
BspEI TCCGGA 2 cut(s) 2114, 2803
BspHI TCATGA 2 cut(s) 505, 1261
BspLI GGNNCC 4 cut(s) 88, 609, 2369, 3461
BspMI ACCTGC 1 cut(s) 4156
BspOI GCTAGC 2 cut(s) 914, 3173
BspPI GGATC 8 cut(s) 188, 934, 1965, 2423, 2500, 2726, 3608, 3632
BspQI GCTCTTC 2 cut(s) 1755, 4134
BspT104I TTCGAA 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 607
BspTNI GGTCTC 3 cut(s) 129, 388, 2557
BsrBI CCGCTC 3 cut(s) 171, 198, 718
BsrDI GCAATG 6 cut(s) 1315, 1449, 2220, 2545, 3825, 3882
BsrFI RCCGGY 4 cut(s) 589, 871, 1701, 2364
BsrGI TGTACA 2 cut(s) 98, 4208
BsrI ACTGG 6 cut(s) 216, 515, 1516, 2099, 2506, 3313
BssAI RCCGGY 4 cut(s) 589, 871, 1701, 2364
BssNI GRCGYC 2 cut(s) 869, 3081
BssSI CACGAG 2 cut(s) 703, 766
BssT1I CCWWGG 4 cut(s) 176, 1027, 2471, 3978
Bst2BI CACGAG 2 cut(s) 703, 766
Bst2UI CCWGG 3 cut(s) 2013, 2433, 2596
Bst6I CTCTTC 8 cut(s) 236, 1032, 1116, 1755, 2209, 3242, 4134, 4157
BstACI GRCGYC 2 cut(s) 869, 3081
BstAUI TGTACA 2 cut(s) 98, 4208
BstBAI YACGTR 2 cut(s) 632, 3523
BstBI TTCGAA 1 cut(s) 489
BstDEI CTNAG 8 cut(s) 893, 2226, 3121, 3135, 3152, 3258, 3340, 4031
BstF5I GGATG 6 cut(s) 1808, 2467, 3038, 3348, 3660, 4038
BstHHI GCGC 2 cut(s) 1672, 4051
BstMAI GTCTC 6 cut(s) 129, 388, 2204, 2557, 2805, 2925
BstNI CCWGG 3 cut(s) 2013, 2433, 2596
BstNSI RCATGY 3 cut(s) 1574, 2001, 4209
BstSFI CTRYAG 1 cut(s) 1623
BstV1I GCAGC 5 cut(s) 1757, 2762, 2784, 3125, 4111
BstV2I GAAGAC 2 cut(s) 264, 3306
BstX2I RGATCY 6 cut(s) 926, 1294, 2492, 3313, 3624, 3670
BstXI CCANNNNNNTGG 1 cut(s) 1405
BstYI RGATCY 6 cut(s) 926, 1294, 2492, 3313, 3624, 3670
Bsu15I ATCGAT 1 cut(s) 22
BsuI GTATCC 3 cut(s) 1557, 2230, 3933
BsuTUI ATCGAT 1 cut(s) 22
BtsCI GGATG 6 cut(s) 1808, 2467, 3038, 3348, 3660, 4038
BtsI GCAGTG 1 cut(s) 2991
BtsIMutI CAGTG 6 cut(s) 33, 1509, 2739, 2753, 2991, 3306
BveI ACCTGC 1 cut(s) 4156
CciI TCATGA 2 cut(s) 505, 1261
CfoI GCGC 2 cut(s) 1672, 4051
Cfr10I RCCGGY 4 cut(s) 589, 871, 1701, 2364
Cfr13I GGNCC 9 cut(s) 60, 155, 173, 425, 2344, 2367, 2481, 2714, 4078
Cfr9I CCCGGG 3 cut(s) 82, 2398, 2783
ClaI ATCGAT 1 cut(s) 22
CpoI CGGWCCG 2 cut(s) 2481, 4078
CseI GACGC 2 cut(s) 877, 916
Csp6I GTAC 6 cut(s) 99, 219, 608, 629, 3771, 4209
CspAI ACCGGT 1 cut(s) 2364
CspI CGGWCCG 2 cut(s) 2481, 4078
CviQI GTAC 6 cut(s) 99, 219, 608, 629, 3771, 4209
DdeI CTNAG 8 cut(s) 893, 2226, 3121, 3135, 3152, 3258, 3340, 4031
DraI TTTAAA 2 cut(s) 1203, 1372
DrdI GACNNNNNNGTC 4 cut(s) 67, 423, 2099, 2712
DriI GACNNNNNGTC 2 cut(s) 2350, 2671
DseDI GACNNNNNNGTC 4 cut(s) 67, 423, 2099, 2712
EaeI YGGCCR 4 cut(s) 1024, 1703, 2503, 3943
Eam1104I CTCTTC 8 cut(s) 236, 1032, 1116, 1755, 2209, 3242, 4134, 4157
Eam1105I GACNNNNNGTC 2 cut(s) 2350, 2671
EarI CTCTTC 8 cut(s) 236, 1032, 1116, 1755, 2209, 3242, 4134, 4157
EciI GGCGGA 2 cut(s) 181, 2494
Ecl136II GAGCTC 1 cut(s) 2468
Eco130I CCWWGG 4 cut(s) 176, 1027, 2471, 3978
Eco147I AGGCCT 3 cut(s) 41, 1984, 2145
Eco24I GRGCYC 2 cut(s) 2012, 2470
Eco31I GGTCTC 3 cut(s) 129, 388, 2557
Eco32I GATATC 2 cut(s) 267, 4201
Eco47I GGWCC 7 cut(s) 60, 425, 2344, 2367, 2481, 2714, 4078
Eco53kI GAGCTC 1 cut(s) 2468
Eco57I CTGAAG 1 cut(s) 4119
Eco88I CYCGRG 4 cut(s) 82, 115, 2398, 2783
EcoICRI GAGCTC 1 cut(s) 2468
EcoO109I RGGNCCY 1 cut(s) 155
EcoRI GAATTC 1 cut(s) 2619
EcoRII CCWGG 3 cut(s) 2011, 2431, 2594
EcoRV GATATC 2 cut(s) 267, 4201
EcoT14I CCWWGG 4 cut(s) 176, 1027, 2471, 3978
EcoT22I ATGCAT 1 cut(s) 4040
EcoT38I GRGCYC 2 cut(s) 2012, 2470
ErhI CCWWGG 4 cut(s) 176, 1027, 2471, 3978
Esp3I CGTCTC 1 cut(s) 2925
FaqI GGGAC 9 cut(s) 200, 767, 1414, 1592, 2105, 2353, 2371, 2654, 3065
FauI CCCGC 2 cut(s) 711, 1777
FauNDI CATATG 3 cut(s) 103, 1417, 3793
FblI GTMKAC 1 cut(s) 2934
Fnu4HI GCNGC 6 cut(s) 172, 1746, 2773, 2776, 3114, 4125
FokI GGATG 6 cut(s) 1795, 2474, 3045, 3335, 3647, 4025
FriOI GRGCYC 2 cut(s) 2012, 2470
Fsp4HI GCNGC 6 cut(s) 172, 1746, 2773, 2776, 3114, 4125
GlaI GCGC 2 cut(s) 1671, 4050
GluI GCNGC 6 cut(s) 172, 1746, 2773, 2776, 3114, 4125
GsuI CTGGAG 2 cut(s) 32, 3330
HgaI GACGC 2 cut(s) 877, 916
HhaI GCGC 2 cut(s) 1672, 4051
Hin1I GRCGYC 2 cut(s) 869, 3081
Hin6I GCGC 2 cut(s) 1670, 4049
HinP1I GCGC 2 cut(s) 1670, 4049
HincII GTYRAC 3 cut(s) 250, 2586, 2746
HindII GTYRAC 3 cut(s) 250, 2586, 2746
HindIII AAGCTT 2 cut(s) 657, 2228
HphI GGTGA 7 cut(s) 43, 212, 1749, 1829, 2572, 2905, 3798
Hpy166II GTNNAC 8 cut(s) 203, 250, 303, 1912, 2344, 2586, 2746, 2935
Hpy8I GTNNAC 8 cut(s) 203, 250, 303, 1912, 2344, 2586, 2746, 2935
Hpy99I CGWCG 4 cut(s) 871, 910, 2352, 3086
HpyCH4IV ACGT 4 cut(s) 631, 1216, 3081, 3522
HpyF3I CTNAG 8 cut(s) 893, 2226, 3121, 3135, 3152, 3258, 3340, 4031
HpySE526I ACGT 4 cut(s) 631, 1216, 3081, 3522
Hsp92I GRCGYC 2 cut(s) 869, 3081
HspAI GCGC 2 cut(s) 1670, 4049
Kpn2I TCCGGA 2 cut(s) 2114, 2803
KpnI GGTACC 1 cut(s) 611
KroI GCCGGC 1 cut(s) 871
KroNI GCCGGC 1 cut(s) 873
LguI GCTCTTC 2 cut(s) 1755, 4134
LmnI GCTCC 8 cut(s) 93, 168, 195, 595, 1378, 1823, 2465, 2473
Lsp1109I GCAGC 5 cut(s) 1757, 2762, 2784, 3125, 4111
LweI GCATC 9 cut(s) 802, 1732, 1931, 3100, 3357, 3495, 4025, 4047, 4111
MaeII ACGT 4 cut(s) 631, 1216, 3081, 3522
MaeIII GTNAC 9 cut(s) 434, 440, 931, 2439, 2521, 2560, 2723, 3037, 3804
MbiI CCGCTC 3 cut(s) 171, 198, 718
MflI RGATCY 6 cut(s) 926, 1294, 2492, 3313, 3624, 3670
MhlI GDGCHC 4 cut(s) 1198, 2012, 2470, 3574
MlsI TGGCCA 3 cut(s) 1026, 2505, 3945
MluNI TGGCCA 3 cut(s) 1026, 2505, 3945
MlyI GAGTC 8 cut(s) 222, 287, 411, 608, 820, 1160, 1786, 3296
Mox20I TGGCCA 3 cut(s) 1026, 2505, 3945
Mph1103I ATGCAT 1 cut(s) 4040
MroI TCCGGA 2 cut(s) 2114, 2803
MroNI GCCGGC 1 cut(s) 871
MroXI GAANNNNTTC 2 cut(s) 1056, 4171
MscI TGGCCA 3 cut(s) 1026, 2505, 3945
Msp20I TGGCCA 3 cut(s) 1026, 2505, 3945
MspA1I CMGCKG 5 cut(s) 1815, 2775, 3116, 3194, 4097
Mva1269I GAATGC 3 cut(s) 1659, 3394, 3904
MvaI CCWGG 3 cut(s) 2013, 2433, 2596
NaeI GCCGGC 1 cut(s) 873
NciI CCSGG 8 cut(s) 83, 84, 91, 483, 2399, 2400, 2784, 2785
NdeI CATATG 3 cut(s) 103, 1417, 3793
NgoMIV GCCGGC 1 cut(s) 871
NheI GCTAGC 2 cut(s) 910, 3169
NlaIV GGNNCC 4 cut(s) 88, 609, 2369, 3461
NmuCI GTSAC 4 cut(s) 440, 2560, 3037, 3804
NsiI ATGCAT 1 cut(s) 4040
NspI RCATGY 3 cut(s) 1574, 2001, 4209
NspV TTCGAA 1 cut(s) 489
PagI TCATGA 2 cut(s) 505, 1261
PceI AGGCCT 3 cut(s) 41, 1984, 2145
PciI ACATGT 2 cut(s) 1570, 4205
PciSI GCTCTTC 2 cut(s) 1755, 4134
PcsI WCGNNNNNNNCGW 1 cut(s) 701
PctI GAATGC 3 cut(s) 1659, 3394, 3904
PdiI GCCGGC 1 cut(s) 873
PdmI GAANNNNTTC 2 cut(s) 1056, 4171
PflMI CCANNNNNTGG 1 cut(s) 2076
PinAI ACCGGT 1 cut(s) 2364
PkrI GCNGC 6 cut(s) 173, 1747, 2774, 2777, 3115, 4126
PleI GAGTC 8 cut(s) 222, 287, 411, 608, 820, 1160, 1786, 3296
PpsI GAGTC 8 cut(s) 222, 287, 411, 608, 820, 1160, 1786, 3296
Ppu21I YACGTR 2 cut(s) 632, 3523
PscI ACATGT 2 cut(s) 1570, 4205
PsiI TTATAA 1 cut(s) 1652
Psp124BI GAGCTC 1 cut(s) 2470
Psp1406I AACGTT 1 cut(s) 1216
Psp6I CCWGG 3 cut(s) 2011, 2431, 2594
PspGI CCWGG 3 cut(s) 2011, 2431, 2594
PspN4I GGNNCC 4 cut(s) 88, 609, 2369, 3461
PspPI GGNCC 9 cut(s) 60, 155, 173, 425, 2344, 2367, 2481, 2714, 4078
PsuI RGATCY 6 cut(s) 926, 1294, 2492, 3313, 3624, 3670
PvuII CAGCTG 3 cut(s) 2775, 3194, 4097
RsaI GTAC 6 cut(s) 100, 220, 609, 630, 3772, 4210
RsaNI GTAC 6 cut(s) 99, 219, 608, 629, 3771, 4209
Rsr2I CGGWCCG 2 cut(s) 2481, 4078
RsrII CGGWCCG 2 cut(s) 2481, 4078
SacI GAGCTC 1 cut(s) 2470
SapI GCTCTTC 2 cut(s) 1755, 4134
SatI GCNGC 6 cut(s) 172, 1746, 2773, 2776, 3114, 4125
Sau96I GGNCC 9 cut(s) 60, 155, 173, 425, 2344, 2367, 2481, 2714, 4078
SchI GAGTC 8 cut(s) 222, 287, 411, 608, 820, 1160, 1786, 3296
SduI GDGCHC 4 cut(s) 1198, 2012, 2470, 3574
SfaNI GCATC 9 cut(s) 802, 1732, 1931, 3100, 3357, 3495, 4025, 4047, 4111
SfcI CTRYAG 1 cut(s) 1623
SfuI TTCGAA 1 cut(s) 489
SinI GGWCC 7 cut(s) 60, 425, 2344, 2367, 2481, 2714, 4078
SmaI CCCGGG 3 cut(s) 84, 2400, 2785
SmlI CTYRAG 2 cut(s) 2258, 3001
SmoI CTYRAG 2 cut(s) 2258, 3001
SpeI ACTAGT 1 cut(s) 3214
SseBI AGGCCT 3 cut(s) 41, 1984, 2145
SsiI CCGC 9 cut(s) 128, 166, 171, 198, 718, 1770, 1815, 2479, 3116
SspI AATATT 3 cut(s) 1368, 3688, 3744
SstI GAGCTC 1 cut(s) 2470
StuI AGGCCT 3 cut(s) 41, 1984, 2145
StyI CCWWGG 4 cut(s) 176, 1027, 2471, 3978
TaiI ACGT 4 cut(s) 634, 1219, 3084, 3525
TaqI TCGA 8 cut(s) 22, 489, 805, 1221, 1271, 3389, 3876, 3990
TaqII GACCGA 3 cut(s) 2361, 2498, 4095
TatI WGTACW 3 cut(s) 98, 3770, 4208
TauI GCSGC 1 cut(s) 174
TscAI CASTG 6 cut(s) 33, 1516, 2746, 2760, 2998, 3313
TseFI GTSAC 4 cut(s) 440, 2560, 3037, 3804
TseI GCWGC 5 cut(s) 1745, 2772, 2775, 3113, 4124
Tsp45I GTSAC 4 cut(s) 440, 2560, 3037, 3804
TspGWI ACGGA 5 cut(s) 68, 2172, 3092, 3380, 4091
TspMI CCCGGG 3 cut(s) 82, 2398, 2783
TspRI CASTG 6 cut(s) 33, 1516, 2746, 2760, 2998, 3313
Van91I CCANNNNNTGG 1 cut(s) 2076
VpaK11BI GGWCC 7 cut(s) 60, 425, 2344, 2367, 2481, 2714, 4078
XapI RAATTY 6 cut(s) 1558, 1688, 1902, 2017, 2619, 3934
XceI RCATGY 3 cut(s) 1574, 2001, 4209
XmaI CCCGGG 3 cut(s) 82, 2398, 2783
XmiI GTMKAC 1 cut(s) 2934
XmnI GAANNNNTTC 2 cut(s) 1056, 4171
ZraI GACGTC 1 cut(s) 3082
Zsp2I ATGCAT 1 cut(s) 4040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.