Rroxscaffold_4G00293110

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000099
Physical Location & Seq
Reverse (-)
13385233 .. 13389229
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00293110.1

Sequence Viewer

Length: 1773 bp
ATGGCCAAGCTTTTGGATACAGGAAATCTTGTTTTACAGCTTTTGCTAGAGAATGGGAGCCAAAGGGTTCTGTGGCAAAGCTTTGATCATCTCTCGCATACATTACTTCCATCTATGAAACTTGGGGTGGACCGGCGGTCAGGGTTTAACTGGTCCCTAACATCTTGGAAGTCTCAAGATGACCCTGAAACCGGGAACTGTACATATGGGATTAATCCTAGTGGTTTCCCGCAGTTTACTTTGTACAAGGGTACGACTCTCGTGAATCGGTATGGAATCGGATCCTGGGGTGTCCAGTATAGCAAAGTGACAACTTATGTGAACAATCAAGATGAGGTAACAGTTACGGATGTTCAAACCGAGTTAATTACTAGGACGGTGCTGGATGATTCGGGAATACTTCTCCGGAGCACATGGAATGAGCGAGCATATGAATGGACTACATTTCAGTCTGTTCCATATGAGCCCTGTGATGAGTATGGAACATGTGGTCCGAATGGCAACTGCAACCCGTACAGCATCACGAGTAAGATCGATTGCACATGCCTACCCGGGTTTGAACCCAAGTCACCAACTGACTGGAACAAGACAATTTGGTCAGGTGGGTGTGTGAAGAAAAATGGAACATCAATGTGTCGAAAAGGAGAAGGGTTTGTAAAGTTGGAAATGATCAAATTGCCAGAGTCGTCCAGGGCACAGGTAAATTTGAATGTGACCTTGAAAGAGTGTGAGCATGAGTGTTTGAGAAATTGCTCATGCACGGCATACTGCAATGTCGTCGATGGGGAAACGAGTAGATGCGTGACATGGTATGGGGACTTAATGGACGCAAAGATGCTTCCGGCTAATGCTCAAGATTTATATGTACGAGTTGATGCTACTGTCTTGGCTCAATATGCAAAGAAGTCGAGGGATTCACTCAGCAAGAAGGGAAGACTGGCAATTTCATCAATAGTACCTGTTCTAGCATTATTTCTACTGCTTTCACTAGTCTATTGGTTGCTAAGGAGGAACAGAAAAGGTAAGCAAAGTGAAAGTAAATATTTATGGTCAACGTCATACTTCGGTGAATCCAACATTAGAGATGTCGATCAAAGTAGAGGAAACTCAGATTTACCATTCTCTGATCTAACAACCATAGCAGCAGCCACAAACAATTTCTCTGATGAGAACAAGCTTGGGACAGGCGGCTTTGGCTCGGTCTATAAGATCTTAGAGCATATAAGCACATTAGGTTTGCATAAGGGAGTTTGTCTTGATTTTGGTCTGGCTAGAATATTCGAGGGGGATCAAAGTGAAGCAAATACAAATCGCGTGGTTGGAACATATGGTTATATGTCACCAGAATATGCAATGCGAGGACTCTTCTCAGTAAAGTCAGATGTATACAGCTTTGGGGTAATAGTATTAGAAATCATTACTGGTCAAAAGAATACTAGTCACCATCCCAGCTCAAGTTTGGTTGAACATGTTTGGAACTCATGGAGAGAAGTTACTGCCTTAGAACTCGTTGATTCATCTCTCAGCGGATCATGCCCTGTCGATGAAGTTCTAAGATGTATCCACATCGCCTTCTTGTGTTTACAAGAGCACGCCACTGACCGGCCAAACATGTCAGAAGTTCTTGTCATGCTGGGTAATGATGCAGCTCTTCCTGAACCAATGCGACCTGCATTTTTAATGGAGAGAAGTAATAACAGTGAAGACTCGTCAAACCGTAAGAGAACTTATTCTGTAAATGAAGTGACACACACCACACTAGAAGCTCGCTAA

Protein Analysis

590

Amino Acids

65.87

Weight (kDa)

5.86

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 1 - 57 3.6e-16 D-mannose binding lectin
S_locus_glycop PF00954 105 - 190 2.3e-17 S-locus glycoprotein domain
PAN_2 PF08276 212 - 276 3.4e-18 PAN-like domain
Pkinase PF00069 419 - 542 3.5e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 420 - 543 1.1e-12 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 547 - 590 4.2e-10 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 595
Acc36I ACCTGC 1 cut(s) 1678
AccI GTMKAC 1 cut(s) 1386
AccII CGCG 1 cut(s) 1314
AccIII TCCGGA 1 cut(s) 405
AciI CCGC 4 cut(s) 136, 230, 1188, 1527
AclWI GGATC 4 cut(s) 276, 289, 1296, 1537
AcoI YGGCCR 2 cut(s) 3, 1604
AcsI RAATTY 1 cut(s) 703
AfaI GTAC 6 cut(s) 202, 245, 253, 515, 867, 957
AfiI CCNNNNNNNGG 2 cut(s) 191, 1602
AflIII ACRYGT 3 cut(s) 485, 1468, 1611
AgsI TTSAA 5 cut(s) 356, 560, 709, 721, 1466
AhdI GACNNNNNGTC 1 cut(s) 136
AhlI ACTAGT 2 cut(s) 988, 1436
AjnI CCWGG 2 cut(s) 284, 689
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
AluBI AGCT 8 cut(s) 10, 40, 81, 1177, 1392, 1452, 1649, 1766
AluI AGCT 8 cut(s) 10, 40, 81, 1177, 1392, 1452, 1649, 1766
Alw21I GWGCWC 2 cut(s) 413, 1593
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 4 cut(s) 276, 289, 1296, 1537
Ama87I CYCGRG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 405
AoxI GGCC 2 cut(s) 3, 1604
ApeKI GCWGC 3 cut(s) 1142, 1145, 1646
ApoI RAATTY 1 cut(s) 703
AseI ATTAAT 1 cut(s) 213
Asp700I GAANNNNTTC 1 cut(s) 1729
AspS9I GGNCC 3 cut(s) 130, 153, 491
AsuC2I CCSGG 3 cut(s) 193, 552, 553
AsuHPI GGTGA 4 cut(s) 561, 1079, 1332, 1433
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 3 cut(s) 130, 153, 491
BaeGI GKGCMC 1 cut(s) 697
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 468
BauI CACGAG 2 cut(s) 260, 523
BbsI GAAGAC 2 cut(s) 940, 1710
Bbv12I GWGCWC 2 cut(s) 413, 1593
BbvI GCAGC 3 cut(s) 1154, 1157, 1658
BccI CCATC 3 cut(s) 118, 776, 1452
BceAI ACGGC 1 cut(s) 777
BcgI CGANNNNNNTGC 4 cut(s) 760, 794, 888, 922
BciT130I CCWGG 2 cut(s) 286, 691
BciVI GTATCC 2 cut(s) 10, 1571
BclI TGATCA 2 cut(s) 85, 669
BcnI CCSGG 3 cut(s) 193, 552, 553
BcoDI GTCTC 1 cut(s) 177
BcuI ACTAGT 2 cut(s) 988, 1436
BfaI CTAG 8 cut(s) 47, 219, 372, 965, 989, 1272, 1437, 1760
BfuAI ACCTGC 1 cut(s) 1678
BfuI GTATCC 2 cut(s) 10, 1571
BglII AGATCT 1 cut(s) 1209
BisI GCNGC 4 cut(s) 1143, 1146, 1189, 1647
BlsI GCNGC 4 cut(s) 1144, 1147, 1190, 1648
Bme1390I CCNGG 5 cut(s) 193, 286, 552, 553, 691
Bme18I GGWCC 3 cut(s) 130, 153, 491
BmeRI GACNNNNNGTC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 3 cut(s) 130, 153, 491
BmiI GGNNCC 3 cut(s) 59, 155, 283
BmrFI CCNGG 5 cut(s) 193, 286, 552, 553, 691
BmsI GCATC 5 cut(s) 528, 788, 825, 865, 1633
BpiI GAAGAC 2 cut(s) 940, 1710
Bpu10I CCTNAGC 1 cut(s) 1004
BpuEI CTTGAG 3 cut(s) 159, 837, 1438
BpuMI CCSGG 3 cut(s) 193, 552, 553
Bsa29I ATCGAT 1 cut(s) 534
BsaBI GATNNNNATC 1 cut(s) 1089
BsaJI CCNNGG 3 cut(s) 285, 551, 690
BsaWI WCCGGW 1 cut(s) 405
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 1602
Bse118I RCCGGY 2 cut(s) 132, 1602
Bse1I ACTGG 5 cut(s) 155, 295, 584, 942, 1426
Bse3DI GCAATG 2 cut(s) 778, 1359
Bse8I GATNNNNATC 1 cut(s) 1089
BseAI TCCGGA 1 cut(s) 405
BseBI CCWGG 2 cut(s) 286, 691
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 3 cut(s) 285, 551, 690
BseGI GGATG 3 cut(s) 355, 391, 1444
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 2 cut(s) 191, 1602
BseMI GCAATG 2 cut(s) 778, 1359
BseMII CTCAG 4 cut(s) 934, 1122, 1383, 1537
BseNI ACTGG 5 cut(s) 155, 295, 584, 942, 1426
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 3 cut(s) 1154, 1157, 1658
BseYI CCCAGC 2 cut(s) 1448, 1633
Bsh1236I CGCG 1 cut(s) 1314
BshFI GGCC 2 cut(s) 5, 1606
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 2 cut(s) 413, 1593
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 6 cut(s) 133, 192, 406, 552, 842, 1603
BslFI GGGAC 3 cut(s) 139, 830, 1195
BslI CCNNNNNNNGG 2 cut(s) 191, 1602
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 3 cut(s) 139, 830, 1195
BsnI GGCC 2 cut(s) 5, 1606
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 4 cut(s) 413, 468, 697, 1593
Bsp13I TCCGGA 1 cut(s) 405
Bsp1407I TGTACA 2 cut(s) 200, 243
Bsp143I GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
BspACI CCGC 4 cut(s) 136, 230, 1188, 1527
BspANI GGCC 2 cut(s) 5, 1606
BspCNI CTCAG 4 cut(s) 933, 1121, 1382, 1536
BspDI ATCGAT 1 cut(s) 534
BspEI TCCGGA 1 cut(s) 405
BspFNI CGCG 1 cut(s) 1314
BspLI GGNNCC 3 cut(s) 59, 155, 283
BspMI ACCTGC 1 cut(s) 1678
BspPI GGATC 4 cut(s) 276, 289, 1296, 1537
BspQI GCTCTTC 1 cut(s) 1656
BsrDI GCAATG 2 cut(s) 778, 1359
BsrFI RCCGGY 2 cut(s) 132, 1602
BsrGI TGTACA 2 cut(s) 200, 243
BsrI ACTGG 5 cut(s) 155, 295, 584, 942, 1426
BssAI RCCGGY 2 cut(s) 132, 1602
BssECI CCNNGG 3 cut(s) 285, 551, 690
BssMI GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
BssNAI GTATAC 1 cut(s) 1387
BssSI CACGAG 2 cut(s) 260, 523
Bst1107I GTATAC 1 cut(s) 1387
Bst2BI CACGAG 2 cut(s) 260, 523
Bst2UI CCWGG 2 cut(s) 286, 691
Bst4CI ACNGT 6 cut(s) 200, 343, 379, 883, 1700, 1718
Bst6I CTCTTC 2 cut(s) 1370, 1656
BstAUI TGTACA 2 cut(s) 200, 243
BstC8I GCNNGC 3 cut(s) 426, 1593, 1768
BstDEI CTNAG 8 cut(s) 920, 1004, 1108, 1213, 1369, 1501, 1523, 1553
BstF5I GGATG 3 cut(s) 355, 391, 1444
BstFNI CGCG 1 cut(s) 1314
BstKTI GATC 9 cut(s) 88, 284, 534, 672, 1093, 1129, 1212, 1291, 1532
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
BstMWI GCNNNNNNNGC 3 cut(s) 896, 1194, 1533
BstNI CCWGG 2 cut(s) 286, 691
BstNSI RCATGY 4 cut(s) 489, 546, 1472, 1615
BstSCI CCNGG 5 cut(s) 191, 284, 550, 551, 689
BstSLI GKGCMC 1 cut(s) 697
BstUI CGCG 1 cut(s) 1314
BstV1I GCAGC 3 cut(s) 1154, 1157, 1658
BstV2I GAAGAC 2 cut(s) 940, 1710
BstX2I RGATCY 2 cut(s) 281, 1209
BstXI CCANNNNNNTGG 2 cut(s) 13, 579
BstYI RGATCY 2 cut(s) 281, 1209
BstZ17I GTATAC 1 cut(s) 1387
Bsu15I ATCGAT 1 cut(s) 534
BsuI GTATCC 2 cut(s) 10, 1571
BsuRI GGCC 2 cut(s) 5, 1606
BsuTUI ATCGAT 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 1552
BtsCI GGATG 3 cut(s) 355, 391, 1444
BtsIMutI CAGTG 2 cut(s) 1596, 1705
BveI ACCTGC 1 cut(s) 1678
Cac8I GCNNGC 3 cut(s) 426, 1593, 1768
Cfr10I RCCGGY 2 cut(s) 132, 1602
Cfr13I GGNCC 3 cut(s) 130, 153, 491
Cfr9I CCCGGG 1 cut(s) 551
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 1 cut(s) 836
Csp6I GTAC 6 cut(s) 201, 244, 252, 514, 866, 956
CspCI CAANNNNNGTGG 2 cut(s) 1296, 1331
CviQI GTAC 6 cut(s) 201, 244, 252, 514, 866, 956
DdeI CTNAG 8 cut(s) 920, 1004, 1108, 1213, 1369, 1501, 1523, 1553
DpnI GATC 9 cut(s) 87, 283, 533, 671, 1092, 1128, 1211, 1290, 1531
DpnII GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
DrdI GACNNNNNNGTC 1 cut(s) 595
DriI GACNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 2 cut(s) 3, 1604
Eam1104I CTCTTC 2 cut(s) 1370, 1656
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 2 cut(s) 1370, 1656
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 3 cut(s) 130, 153, 491
Eco88I CYCGRG 1 cut(s) 551
EcoRII CCWGG 2 cut(s) 284, 689
EcoT38I GRGCYC 1 cut(s) 468
FaqI GGGAC 3 cut(s) 139, 830, 1195
FauI CCCGC 1 cut(s) 237
FauNDI CATATG 4 cut(s) 205, 430, 460, 1327
FbaI TGATCA 2 cut(s) 85, 669
FblI GTMKAC 1 cut(s) 1386
Fnu4HI GCNGC 4 cut(s) 1143, 1146, 1189, 1647
FokI GGATG 3 cut(s) 362, 398, 1431
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 4 cut(s) 1143, 1146, 1189, 1647
FspBI CTAG 8 cut(s) 47, 219, 372, 965, 989, 1272, 1437, 1760
GluI GCNGC 4 cut(s) 1143, 1146, 1189, 1647
GsaI CCCAGC 2 cut(s) 1452, 1637
HaeIII GGCC 2 cut(s) 5, 1606
HapII CCGG 6 cut(s) 133, 192, 406, 552, 842, 1603
HgaI GACGC 1 cut(s) 836
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 3 cut(s) 8, 79, 1175
HpaII CCGG 6 cut(s) 133, 192, 406, 552, 842, 1603
HphI GGTGA 4 cut(s) 561, 1079, 1332, 1433
Hpy166II GTNNAC 6 cut(s) 130, 237, 322, 1053, 1387, 1583
Hpy188I TCNGA 7 cut(s) 281, 495, 1111, 1126, 1165, 1381, 1618
Hpy188III TCNNGA 9 cut(s) 176, 262, 329, 393, 406, 523, 854, 1256, 1655
Hpy8I GTNNAC 6 cut(s) 130, 237, 322, 1053, 1387, 1583
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 3 cut(s) 641, 922, 1582
HpyCH4III ACNGT 6 cut(s) 200, 343, 379, 883, 1700, 1718
HpyCH4IV ACGT 1 cut(s) 1055
HpyCH4V TGCA 9 cut(s) 507, 540, 759, 771, 899, 1240, 1352, 1646, 1673
HpyF10VI GCNNNNNNNGC 3 cut(s) 896, 1194, 1533
HpyF3I CTNAG 8 cut(s) 920, 1004, 1108, 1213, 1369, 1501, 1523, 1553
HpySE526I ACGT 1 cut(s) 1055
Kpn2I TCCGGA 1 cut(s) 405
Ksp22I TGATCA 2 cut(s) 85, 669
Kzo9I GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
LguI GCTCTTC 1 cut(s) 1656
LmnI GCTCC 2 cut(s) 57, 408
Lsp1109I GCAGC 3 cut(s) 1154, 1157, 1658
LweI GCATC 5 cut(s) 528, 788, 825, 865, 1633
MaeI CTAG 8 cut(s) 47, 219, 372, 965, 989, 1272, 1437, 1760
MaeII ACGT 1 cut(s) 1055
MalI GATC 9 cut(s) 87, 283, 533, 671, 1092, 1128, 1211, 1290, 1531
MboI GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
MboII GAAGA 5 cut(s) 625, 945, 1357, 1643, 1715
MflI RGATCY 2 cut(s) 281, 1209
MhlI GDGCHC 4 cut(s) 413, 468, 697, 1593
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 366, 591, 674, 703, 748, 942, 1156
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 250, 692, 1356, 1700
MmeI TCCRAC 3 cut(s) 642, 1098, 1300
MnlI CCTC 6 cut(s) 328, 903, 1002, 1094, 1276, 1352
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 1729
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 147, 213, 365, 821, 1679
MslI CAYNNNNRTG 2 cut(s) 433, 631
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1527
MspI CCGG 6 cut(s) 133, 192, 406, 552, 842, 1603
MspR9I CCNGG 5 cut(s) 193, 286, 552, 553, 691
MvaI CCWGG 2 cut(s) 286, 691
MvnI CGCG 1 cut(s) 1314
MwoI GCNNNNNNNGC 3 cut(s) 896, 1194, 1533
NciI CCSGG 3 cut(s) 193, 552, 553
NdeI CATATG 4 cut(s) 205, 430, 460, 1327
NdeII GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
NlaIV GGNNCC 3 cut(s) 59, 155, 283
NmuCI GTSAC 7 cut(s) 307, 567, 712, 802, 1338, 1439, 1744
NspI RCATGY 4 cut(s) 489, 546, 1472, 1615
PciI ACATGT 3 cut(s) 485, 1468, 1611
PciSI GCTCTTC 1 cut(s) 1656
PdmI GAANNNNTTC 1 cut(s) 1729
PfeI GAWTC 6 cut(s) 265, 276, 389, 914, 1070, 1514
PkrI GCNGC 4 cut(s) 1144, 1147, 1190, 1648
PleI GAGTC 4 cut(s) 250, 691, 1356, 1700
PpsI GAGTC 4 cut(s) 250, 691, 1356, 1700
PscI ACATGT 3 cut(s) 485, 1468, 1611
PshBI ATTAAT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 284, 689
PspFI CCCAGC 2 cut(s) 1448, 1633
PspGI CCWGG 2 cut(s) 284, 689
PspN4I GGNNCC 3 cut(s) 59, 155, 283
PspPI GGNCC 3 cut(s) 130, 153, 491
PsuI RGATCY 2 cut(s) 281, 1209
RsaI GTAC 6 cut(s) 202, 245, 253, 515, 867, 957
RsaNI GTAC 6 cut(s) 201, 244, 252, 514, 866, 956
RseI CAYNNNNRTG 2 cut(s) 433, 631
SapI GCTCTTC 1 cut(s) 1656
SaqAI TTAA 5 cut(s) 147, 213, 365, 821, 1679
SatI GCNGC 4 cut(s) 1143, 1146, 1189, 1647
Sau3AI GATC 9 cut(s) 85, 281, 531, 669, 1090, 1126, 1209, 1288, 1529
Sau96I GGNCC 3 cut(s) 130, 153, 491
SchI GAGTC 4 cut(s) 250, 692, 1356, 1700
ScrFI CCNGG 5 cut(s) 193, 286, 552, 553, 691
SduI GDGCHC 4 cut(s) 413, 468, 697, 1593
SfaNI GCATC 5 cut(s) 528, 788, 825, 865, 1633
SinI GGWCC 3 cut(s) 130, 153, 491
SmaI CCCGGG 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 433, 631
SmlI CTYRAG 3 cut(s) 174, 852, 1453
SmoI CTYRAG 3 cut(s) 174, 852, 1453
SpeI ACTAGT 2 cut(s) 988, 1436
Sse9I AATT 7 cut(s) 366, 591, 674, 703, 748, 942, 1156
SsiI CCGC 4 cut(s) 136, 230, 1188, 1527
SspI AATATT 2 cut(s) 1043, 1278
SspMI CTAG 8 cut(s) 47, 219, 372, 965, 989, 1272, 1437, 1760
StyD4I CCNGG 5 cut(s) 191, 284, 550, 551, 689
TaaI ACNGT 6 cut(s) 200, 343, 379, 883, 1700, 1718
TaiI ACGT 1 cut(s) 1058
TaqI TCGA 7 cut(s) 534, 637, 780, 908, 1089, 1281, 1542
TaqII GACCGA 1 cut(s) 1189
TasI AATT 7 cut(s) 366, 591, 674, 703, 748, 942, 1156
TatI WGTACW 2 cut(s) 200, 243
TauI GCSGC 1 cut(s) 1191
TfiI GAWTC 6 cut(s) 265, 276, 389, 914, 1070, 1514
Tru1I TTAA 5 cut(s) 147, 213, 365, 821, 1679
Tru9I TTAA 5 cut(s) 147, 213, 365, 821, 1679
TscAI CASTG 2 cut(s) 1603, 1705
TseFI GTSAC 7 cut(s) 307, 567, 712, 802, 1338, 1439, 1744
TseI GCWGC 3 cut(s) 1142, 1145, 1646
Tsp45I GTSAC 7 cut(s) 307, 567, 712, 802, 1338, 1439, 1744
TspDTI ATGAA 6 cut(s) 131, 447, 936, 1506, 1560, 1755
TspGWI ACGGA 1 cut(s) 362
TspMI CCCGGG 1 cut(s) 551
TspRI CASTG 2 cut(s) 1603, 1705
VpaK11BI GGWCC 3 cut(s) 130, 153, 491
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 1 cut(s) 703
XceI RCATGY 4 cut(s) 489, 546, 1472, 1615
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XmaI CCCGGG 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 1386
XmnI GAANNNNTTC 1 cut(s) 1729
XspI CTAG 8 cut(s) 47, 219, 372, 965, 989, 1272, 1437, 1760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.