Rw5G022450

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000099
Physical Location & Seq
Reverse (-)
30994482 .. 30999516
5035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022450.1

Sequence Viewer

Length: 2484 bp
ATGAGTTCCAGTATCGAAGCATTTATCCAGATATTGCTTCTCTTCCTTCTTCTCCCCTCTAGCATTTGCCAGCTTCCCCTTGACGCTCTTACGCCAAACCGTGCCATCAGAGACGGCGACGTTTTAGTCTCTAGCTCGCAAATCTTTGCACTAGGATTCTTCAGCCCCGGAAATTCTCACCACCGCTACGCTGGAGTTTGGTATAACAAGATTCCAGAGAAAGCCATTGTCTGGGTTGCGAACAGAGACAACCCAGTCAATGATTCCTACGGAGTGCTATCCATCAACGGTGATGGAGGCCTTGTCATACATGGAAAGGACCCAAGTACCCCTCTTTGGTCCGCAAACGTTACTCTTTCTTCACCAAACAATTTCACAGCCAAGCTTTTGGATACAGGAAATCTTGTTTTACTTGAGAATAGTAGCCAAAGAGTTGTGTGGGAAGGCTTTGATTACCCCTCAGATACACTGCTTCCCTTCATGAAGATTGGGCTGAACCGGCGGTCCGGGTTGGAGAAGCACCTAACATCTTGGAAATCAAAAGATGACCCGGGAACCGGGAGTTGTACATATGGGATAGACCCGATGGGGGTTCCGCAGTTGTTTGTGCGCAAGGGTAGAGACGCATTGTTTCGGGTCGGCCCTTGGACTGGGGACAGATTGAGCGGTACCGTTGGAAACTACAATTCAAGTTTTGTCAACAATCAAGACGAGATATCCTTCGTGTATTTTGCTGCTCTGAGGCCTCAGATCGCAAGGGTGGTGATAGATGAATCAGGAATACTTCAAACGTTCGCGTGGAATGATCAATGGATCAAATTCTACTCCTACCCGACTAGTTGGTGTGATTCCTACGGACAGTGCGGTCCAAATACCAACTGTGACCCAGACAAGGACTATAAGTTGGCTTGCACGTGCCTACCTGGGTTCGAATCCAAATCACCTGGTTTGGGTCTTGGTGAGGGTGCATGCATTAGGAAAGCGGAAGTGTCCACATGTCAAAATGGAGAAGGGTTCGTGAAGGTGGCGCGTGTAAACATACCGGACTCGTCTACGGCACGTGTGAACTTGAGTATGAGTTTGGAACAGTGCAAACAAAAGTGCTTGATGGATTGTTCTTGCACAGCTTACACGAGTGCGGATGACCGGGGAGGTGGGATTGGGTGTGTGACATGGCAGGGGGACTTGATAGACACGAAGACTTTCTCTCATGTTGGTCAAGATTTATATGTTCGAGTGGATGCAACTTTTTTAGCTCAATATGCAAAGTCAGGTGGTTCTCTTAGCAAGAAGGCAAGGGTGGCAATTTCACTTGGATCTGTTACAGTTTTTATTCTCTTACTTATCCTTTATTGGTTAGTAAGGATGAAGATGAAGGGTAAGCGTAGGCAAACTAAATGTTCATTCGAACTTACTGCTGGGTCAACCTACTTCGAAGAAGCTACTGGTGGATTAACTCTTGATGATAGTAGAATAAACTCGGAGTTACTATTATTTCATCTAAACACCGTAGCCACCGCCACAAACAATTTCTCCATTGAAAACAAGCTTGGAGAAGGAGGGTTTGGCTCCGTTTATAAGGGGATACTTTACGATGGAAAGGAAATAGCTGTAAAAAGACTATCCAAGTTTTCTAGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTGATTGCAAAACTCCAACACAGAAACCTTGTTAAGATTTTAGGTTGTTGTTTTGAAGATGAAGAGAAGATTCTAATATATGAATACTTGCCAAACAAAAGTTTGGACTCTTTCATCTTTAATGAACCAAGGAGAGCACTTTTAAGTTGGACAAGACGCTTCGAGATTATCTTGGGGATTGCTAGAGGGCTATTATATCTTCATGAAGATTCAAGACTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTTCTATTGGATAATTCTTTGAACCCAAAGATTGCAGATTTTGGTATGGCTAGAATATTTAGAAGGGAGCAAACTGAAGCAAACACAAATCATGTGGTTGGAACATATGGTTATATGTCACCAGAATATGCAATGAAAGGGCTGTTTTCAATAAAGTCTGATGTGTATAGTTTTGGTGTTTTATTGCTAGAAATCATTACTGGCAAAAAGAATGCTGGTTCCTATGAGAAGGATCCATATTCAAATTTGATTGGACATGTTTGGGAGTTATGGAAAGAAGACAGAGCCGTGGAAATCATTGATTCATCTATAGGTGAATCCTACCTTGTTAGTGAAATTATAAGGTGCATTCAAATCGCACTCCTGTGTGTGCAAGAATTTGCGATTGACCGGCCAACCATGTCGGTAGTTGTTTCAATGTTAAGTAATGATGTAGCTTTTACTTCACCAAAACGACCTGCATTTTTACTAAAGAGCATGAGTCCTAGTGGAGAACCATCCAACAACAAAAGAGTTAGTTCAGTAAATGATGTCACATGTACAATTGTAGAAGCTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

827

Amino Acids

91.75

Weight (kDa)

7.1

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 179 4e-33 D-mannose binding lectin
S_locus_glycop PF00954 211 - 313 9.9e-18 S-locus glycoprotein domain
PAN_2 PF08276 333 - 399 1.4e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 512 - 729 5.7e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 512 - 719 7e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1578, 2264
AasI GACNNNNNNGTC 3 cut(s) 125, 254, 864
Acc16I TGCGCA 1 cut(s) 611
Acc36I ACCTGC 1 cut(s) 2389
Acc65I GGTACC 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 668
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 666
AccI GTMKAC 1 cut(s) 1052
AccII CGCG 2 cut(s) 797, 1030
AciI CCGC 9 cut(s) 184, 342, 502, 596, 666, 864, 983, 1139, 1518
AclI AACGTT 2 cut(s) 348, 791
AclWI GGATC 4 cut(s) 821, 1324, 2150, 2163
AcoI YGGCCR 1 cut(s) 2315
AcsI RAATTY 4 cut(s) 172, 818, 2167, 2300
AcuI CTGAAG 2 cut(s) 145, 2019
AcvI CACGTG 2 cut(s) 915, 1061
AfaI GTAC 4 cut(s) 328, 568, 670, 2464
AfiI CCNNNNNNNGG 7 cut(s) 231, 336, 557, 589, 650, 892, 950
AflIII ACRYGT 4 cut(s) 995, 1060, 2179, 2459
AhlI ACTAGT 1 cut(s) 836
AjnI CCWGG 2 cut(s) 922, 943
AjuI GAANNNNNNNTTGG 4 cut(s) 1533, 1548, 1565, 1580
AleI CACNNNNGTG 1 cut(s) 2287
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
Alw21I GWGCWC 1 cut(s) 1810
Alw26I GTCTC 4 cut(s) 105, 133, 240, 615
AlwI GGATC 4 cut(s) 821, 1324, 2150, 2163
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 5 cut(s) 298, 640, 743, 1914, 2315
ApeKI GCWGC 1 cut(s) 734
ApoI RAATTY 4 cut(s) 172, 818, 2167, 2300
Asp700I GAANNNNTTC 2 cut(s) 1202, 1668
Asp718I GGTACC 1 cut(s) 668
AspLEI GCGC 2 cut(s) 612, 1030
AspS9I GGNCC 5 cut(s) 319, 339, 504, 641, 866
AsuC2I CCSGG 6 cut(s) 168, 508, 551, 552, 559, 1148
AsuHPI GGTGA 9 cut(s) 170, 302, 354, 775, 933, 971, 2034, 2249, 2361
AsuII TTCGAA 3 cut(s) 930, 1407, 1434
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 4 cut(s) 319, 339, 504, 866
BaeI ACNNNNGTAYC 2 cut(s) 310, 343
BamHI GGATCC 1 cut(s) 2155
BanI GGYRCC 1 cut(s) 668
BauI CACGAG 1 cut(s) 1132
BbrPI CACGTG 2 cut(s) 915, 1061
BbsI GAAGAC 2 cut(s) 1205, 2208
Bbv12I GWGCWC 1 cut(s) 1810
BbvI GCAGC 1 cut(s) 721
BccI CCATC 7 cut(s) 113, 287, 290, 580, 1102, 1589, 2428
BceAI ACGGC 3 cut(s) 130, 1071, 2195
BcgI CGANNNNNNTGC 4 cut(s) 1397, 1431, 2260, 2294
BciT130I CCWGG 2 cut(s) 924, 945
BciVI GTATCC 2 cut(s) 385, 1578
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 6 cut(s) 168, 508, 551, 552, 559, 1148
BcoDI GTCTC 4 cut(s) 105, 133, 240, 615
BcuI ACTAGT 1 cut(s) 836
BfaI CTAG 9 cut(s) 60, 132, 152, 837, 1635, 1854, 1974, 2111, 2409
BfmI CTRYAG 1 cut(s) 2232
BfuAI ACCTGC 1 cut(s) 2389
BfuI GTATCC 2 cut(s) 385, 1578
BglII AGATCT 1 cut(s) 1906
BisI GCNGC 1 cut(s) 735
BlsI GCNGC 1 cut(s) 736
Bme1390I CCNGG 8 cut(s) 168, 508, 551, 552, 559, 924, 945, 1148
Bme18I GGWCC 4 cut(s) 319, 339, 504, 866
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 5 cut(s) 319, 339, 504, 641, 866
BmiI GGNNCC 7 cut(s) 321, 556, 594, 670, 1570, 2143, 2157
BmrFI CCNGG 8 cut(s) 168, 508, 551, 552, 559, 924, 945, 1148
BmrI ACTGGG 2 cut(s) 248, 660
BmsI GCATC 1 cut(s) 1231
BmuI ACTGGG 2 cut(s) 248, 660
BpiI GAAGAC 2 cut(s) 1205, 2208
BpmI CTGGAG 1 cut(s) 213
Bpu14I TTCGAA 3 cut(s) 930, 1407, 1434
BpuEI CTTGAG 2 cut(s) 434, 1090
BpuMI CCSGG 6 cut(s) 168, 508, 551, 552, 559, 1148
BsaAI YACGTR 2 cut(s) 915, 1061
BsaJI CCNNGG 8 cut(s) 166, 550, 644, 923, 1147, 1639, 1799, 2211
BsaWI WCCGGW 1 cut(s) 1042
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 1517, 1547
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 336, 557, 589, 650, 892, 950
Bse118I RCCGGY 2 cut(s) 498, 2313
Bse1I ACTGG 5 cut(s) 9, 254, 655, 1450, 2128
Bse3DI GCAATG 2 cut(s) 1927, 2061
BseBI CCWGG 2 cut(s) 924, 945
BseDI CCNNGG 8 cut(s) 166, 550, 644, 923, 1147, 1639, 1799, 2211
BseGI GGATG 4 cut(s) 1147, 1246, 1371, 2420
BseLI CCNNNNNNNGG 7 cut(s) 231, 336, 557, 589, 650, 892, 950
BseMI GCAATG 2 cut(s) 1927, 2061
BseMII CTCAG 3 cut(s) 474, 731, 761
BseNI ACTGG 5 cut(s) 9, 254, 655, 1450, 2128
BseXI GCAGC 1 cut(s) 721
BseYI CCCAGC 1 cut(s) 1418
Bsh1236I CGCG 2 cut(s) 797, 1030
BshFI GGCC 5 cut(s) 300, 642, 745, 1916, 2317
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 1810
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 8 cut(s) 168, 499, 507, 551, 558, 1043, 1147, 2314
BslFI GGGAC 2 cut(s) 668, 1196
BslI CCNNNNNNNGG 7 cut(s) 231, 336, 557, 589, 650, 892, 950
BsmAI GTCTC 4 cut(s) 105, 133, 240, 615
BsmBI CGTCTC 2 cut(s) 105, 615
BsmFI GGGAC 2 cut(s) 668, 1196
BsmI GAATGC 2 cut(s) 2140, 2271
BsnI GGCC 5 cut(s) 300, 642, 745, 1916, 2317
BsoBI CYCGRG 1 cut(s) 550
Bsp119I TTCGAA 3 cut(s) 930, 1407, 1434
Bsp1286I GDGCHC 1 cut(s) 1810
Bsp1407I TGTACA 2 cut(s) 566, 2462
Bsp143I GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
BspACI CCGC 9 cut(s) 184, 342, 502, 596, 666, 864, 983, 1139, 1518
BspANI GGCC 5 cut(s) 300, 642, 745, 1916, 2317
BspCNI CTCAG 3 cut(s) 473, 732, 760
BspFNI CGCG 2 cut(s) 797, 1030
BspHI TCATGA 2 cut(s) 480, 1873
BspLI GGNNCC 7 cut(s) 321, 556, 594, 670, 1570, 2143, 2157
BspMI ACCTGC 1 cut(s) 2389
BspPI GGATC 4 cut(s) 821, 1324, 2150, 2163
BspT104I TTCGAA 3 cut(s) 930, 1407, 1434
BspT107I GGYRCC 1 cut(s) 668
BsrBI CCGCTC 1 cut(s) 666
BsrDI GCAATG 2 cut(s) 1927, 2061
BsrFI RCCGGY 2 cut(s) 498, 2313
BsrGI TGTACA 2 cut(s) 566, 2462
BsrI ACTGG 5 cut(s) 9, 254, 655, 1450, 2128
BssAI RCCGGY 2 cut(s) 498, 2313
BssECI CCNNGG 8 cut(s) 166, 550, 644, 923, 1147, 1639, 1799, 2211
BssMI GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
BssSI CACGAG 1 cut(s) 1132
BssT1I CCWWGG 3 cut(s) 644, 1639, 1799
Bst2BI CACGAG 1 cut(s) 1132
Bst2UI CCWGG 2 cut(s) 924, 945
Bst4CI ACNGT 8 cut(s) 101, 290, 673, 861, 881, 1089, 1327, 1510
Bst6I CTCTTC 3 cut(s) 47, 1644, 1728
BstAUI TGTACA 2 cut(s) 566, 2462
BstBAI YACGTR 2 cut(s) 915, 1061
BstBI TTCGAA 3 cut(s) 930, 1407, 1434
BstC8I GCNNGC 5 cut(s) 71, 137, 910, 970, 1918
BstDEI CTNAG 5 cut(s) 460, 740, 747, 1283, 1889
BstDSI CCRYGG 1 cut(s) 2211
BstF5I GGATG 4 cut(s) 1147, 1246, 1371, 2420
BstFNI CGCG 2 cut(s) 797, 1030
BstHHI GCGC 2 cut(s) 612, 1030
BstKTI GATC 6 cut(s) 753, 808, 816, 1319, 1909, 2158
BstMAI GTCTC 4 cut(s) 105, 133, 240, 615
BstMBI GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
BstMWI GCNNNNNNNGC 3 cut(s) 499, 1262, 1301
BstNI CCWGG 2 cut(s) 924, 945
BstNSI RCATGY 4 cut(s) 972, 999, 2183, 2463
BstSCI CCNGG 8 cut(s) 166, 506, 549, 550, 557, 922, 943, 1146
BstSFI CTRYAG 1 cut(s) 2232
BstUI CGCG 2 cut(s) 797, 1030
BstV1I GCAGC 1 cut(s) 721
BstV2I GAAGAC 2 cut(s) 1205, 2208
BstX2I RGATCY 3 cut(s) 1316, 1906, 2155
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 3 cut(s) 1316, 1906, 2155
BsuI GTATCC 2 cut(s) 385, 1578
BsuRI GGCC 5 cut(s) 300, 642, 745, 1916, 2317
BtgI CCRYGG 1 cut(s) 2211
BtsCI GGATG 4 cut(s) 1147, 1246, 1371, 2420
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 3 cut(s) 467, 866, 1094
BveI ACCTGC 1 cut(s) 2389
Cac8I GCNNGC 5 cut(s) 71, 137, 910, 970, 1918
CciI TCATGA 2 cut(s) 480, 1873
CfoI GCGC 2 cut(s) 612, 1030
Cfr10I RCCGGY 2 cut(s) 498, 2313
Cfr13I GGNCC 5 cut(s) 319, 339, 504, 641, 866
Cfr9I CCCGGG 1 cut(s) 550
CpoI CGGWCCG 1 cut(s) 504
CseI GACGC 3 cut(s) 92, 632, 1836
CsiI ACCWGGT 1 cut(s) 943
Csp6I GTAC 4 cut(s) 327, 567, 669, 2463
CspCI CAANNNNNGTGG 2 cut(s) 1998, 2033
CspI CGGWCCG 1 cut(s) 504
CviQI GTAC 4 cut(s) 327, 567, 669, 2463
DdeI CTNAG 5 cut(s) 460, 740, 747, 1283, 1889
DpnI GATC 6 cut(s) 752, 807, 815, 1318, 1908, 2157
DpnII GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
DrdI GACNNNNNNGTC 3 cut(s) 125, 254, 864
DseDI GACNNNNNNGTC 3 cut(s) 125, 254, 864
EaeI YGGCCR 1 cut(s) 2315
Eam1104I CTCTTC 3 cut(s) 47, 1644, 1728
EarI CTCTTC 3 cut(s) 47, 1644, 1728
Eco130I CCWWGG 3 cut(s) 644, 1639, 1799
Eco147I AGGCCT 2 cut(s) 300, 745
Eco32I GATATC 1 cut(s) 717
Eco47I GGWCC 4 cut(s) 319, 339, 504, 866
Eco57I CTGAAG 2 cut(s) 145, 2019
Eco72I CACGTG 2 cut(s) 915, 1061
Eco88I CYCGRG 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 2 cut(s) 922, 943
EcoRV GATATC 1 cut(s) 717
EcoT14I CCWWGG 3 cut(s) 644, 1639, 1799
EcoT22I ATGCAT 1 cut(s) 974
ErhI CCWWGG 3 cut(s) 644, 1639, 1799
Esp3I CGTCTC 2 cut(s) 105, 615
FaqI GGGAC 2 cut(s) 668, 1196
FauNDI CATATG 2 cut(s) 571, 2029
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 1052
Fnu4HI GCNGC 1 cut(s) 735
FokI GGATG 4 cut(s) 1154, 1253, 1378, 2407
Fsp4HI GCNGC 1 cut(s) 735
FspBI CTAG 9 cut(s) 60, 132, 152, 837, 1635, 1854, 1974, 2111, 2409
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 2 cut(s) 611, 1029
GluI GCNGC 1 cut(s) 735
GsaI CCCAGC 1 cut(s) 1422
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 5 cut(s) 300, 642, 745, 1916, 2317
HapII CCGG 8 cut(s) 168, 499, 507, 551, 558, 1043, 1147, 2314
HgaI GACGC 3 cut(s) 92, 632, 1836
HhaI GCGC 2 cut(s) 612, 1030
Hin6I GCGC 2 cut(s) 610, 1028
HinP1I GCGC 2 cut(s) 610, 1028
HincII GTYRAC 2 cut(s) 700, 1425
HindII GTYRAC 2 cut(s) 700, 1425
HindIII AAGCTT 2 cut(s) 383, 1547
HpaII CCGG 8 cut(s) 168, 499, 507, 551, 558, 1043, 1147, 2314
HphI GGTGA 9 cut(s) 170, 302, 354, 775, 933, 971, 2034, 2249, 2361
Hpy166II GTNNAC 6 cut(s) 700, 993, 1036, 1053, 1066, 1425
Hpy188I TCNGA 7 cut(s) 110, 463, 741, 750, 1483, 1674, 2083
Hpy8I GTNNAC 6 cut(s) 700, 993, 1036, 1053, 1066, 1425
Hpy99I CGWCG 1 cut(s) 122
HpyCH4III ACNGT 8 cut(s) 101, 290, 673, 861, 881, 1089, 1327, 1510
HpyCH4IV ACGT 5 cut(s) 120, 348, 791, 914, 1060
HpyF10VI GCNNNNNNNGC 3 cut(s) 499, 1262, 1301
HpyF3I CTNAG 5 cut(s) 460, 740, 747, 1283, 1889
HpySE526I ACGT 5 cut(s) 120, 348, 791, 914, 1060
HspAI GCGC 2 cut(s) 610, 1028
KpnI GGTACC 1 cut(s) 672
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
LmnI GCTCC 2 cut(s) 1574, 1990
Lsp1109I GCAGC 1 cut(s) 721
LweI GCATC 1 cut(s) 1231
MabI ACCWGGT 1 cut(s) 943
MaeI CTAG 9 cut(s) 60, 132, 152, 837, 1635, 1854, 1974, 2111, 2409
MaeII ACGT 5 cut(s) 120, 348, 791, 914, 1060
MaeIII GTNAC 7 cut(s) 349, 881, 1168, 1321, 1485, 2040, 2455
MalI GATC 6 cut(s) 752, 807, 815, 1318, 1908, 2157
MbiI CCGCTC 1 cut(s) 666
MboI GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
MfeI CAATTG 1 cut(s) 2466
MflI RGATCY 3 cut(s) 1316, 1906, 2155
MhlI GDGCHC 1 cut(s) 1810
MlyI GAGTC 3 cut(s) 1040, 1772, 2413
MmeI TCCRAC 6 cut(s) 492, 655, 1711, 1799, 2002, 2448
Mph1103I ATGCAT 1 cut(s) 974
MroXI GAANNNNTTC 2 cut(s) 1202, 1668
MseI TTAA 6 cut(s) 1454, 1656, 1704, 1791, 1814, 2345
MslI CAYNNNNRTG 1 cut(s) 2287
MspI CCGG 8 cut(s) 168, 499, 507, 551, 558, 1043, 1147, 2314
MspR9I CCNGG 8 cut(s) 168, 508, 551, 552, 559, 924, 945, 1148
MunI CAATTG 1 cut(s) 2466
Mva1269I GAATGC 2 cut(s) 2140, 2271
MvaI CCWGG 2 cut(s) 924, 945
MvnI CGCG 2 cut(s) 797, 1030
MwoI GCNNNNNNNGC 3 cut(s) 499, 1262, 1301
NciI CCSGG 6 cut(s) 168, 508, 551, 552, 559, 1148
NdeI CATATG 2 cut(s) 571, 2029
NdeII GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
NlaIV GGNNCC 7 cut(s) 321, 556, 594, 670, 1570, 2143, 2157
NmuCI GTSAC 4 cut(s) 881, 1168, 2040, 2455
NsbI TGCGCA 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 974
NspI RCATGY 4 cut(s) 972, 999, 2183, 2463
NspV TTCGAA 3 cut(s) 930, 1407, 1434
OliI CACNNNNGTG 1 cut(s) 2287
PaeI GCATGC 1 cut(s) 972
PagI TCATGA 2 cut(s) 480, 1873
PceI AGGCCT 2 cut(s) 300, 745
PciI ACATGT 3 cut(s) 995, 2179, 2459
PctI GAATGC 2 cut(s) 2140, 2271
PdmI GAANNNNTTC 2 cut(s) 1202, 1668
PflMI CCANNNNNTGG 1 cut(s) 231
PkrI GCNGC 1 cut(s) 736
PleI GAGTC 3 cut(s) 1040, 1772, 2412
PmaCI CACGTG 2 cut(s) 915, 1061
PmlI CACGTG 2 cut(s) 915, 1061
PpsI GAGTC 3 cut(s) 1040, 1772, 2412
Ppu21I YACGTR 2 cut(s) 915, 1061
PpuMI RGGWCCY 1 cut(s) 319
PscI ACATGT 3 cut(s) 995, 2179, 2459
PsiI TTATAA 2 cut(s) 1578, 2264
Psp1406I AACGTT 2 cut(s) 348, 791
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 922, 943
PspCI CACGTG 2 cut(s) 915, 1061
PspFI CCCAGC 1 cut(s) 1418
PspGI CCWGG 2 cut(s) 922, 943
PspN4I GGNNCC 7 cut(s) 321, 556, 594, 670, 1570, 2143, 2157
PspPI GGNCC 5 cut(s) 319, 339, 504, 641, 866
PspPPI RGGWCCY 1 cut(s) 319
PsuI RGATCY 3 cut(s) 1316, 1906, 2155
RsaI GTAC 4 cut(s) 328, 568, 670, 2464
RsaNI GTAC 4 cut(s) 327, 567, 669, 2463
RseI CAYNNNNRTG 1 cut(s) 2287
Rsr2I CGGWCCG 1 cut(s) 504
RsrII CGGWCCG 1 cut(s) 504
SaqAI TTAA 6 cut(s) 1454, 1656, 1704, 1791, 1814, 2345
SatI GCNGC 1 cut(s) 735
Sau3AI GATC 6 cut(s) 750, 805, 813, 1316, 1906, 2155
Sau96I GGNCC 5 cut(s) 319, 339, 504, 641, 866
SchI GAGTC 3 cut(s) 1040, 1772, 2413
ScrFI CCNGG 8 cut(s) 168, 508, 551, 552, 559, 924, 945, 1148
SduI GDGCHC 1 cut(s) 1810
SexAI ACCWGGT 1 cut(s) 943
SfaNI GCATC 1 cut(s) 1231
SfcI CTRYAG 1 cut(s) 2232
SfuI TTCGAA 3 cut(s) 930, 1407, 1434
SinI GGWCC 4 cut(s) 319, 339, 504, 866
SmaI CCCGGG 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 2287
SmlI CTYRAG 2 cut(s) 413, 1069
SmoI CTYRAG 2 cut(s) 413, 1069
SpeI ACTAGT 1 cut(s) 836
SphI GCATGC 1 cut(s) 972
SseBI AGGCCT 2 cut(s) 300, 745
SsiI CCGC 9 cut(s) 184, 342, 502, 596, 666, 864, 983, 1139, 1518
SspI AATATT 1 cut(s) 1980
SspMI CTAG 9 cut(s) 60, 132, 152, 837, 1635, 1854, 1974, 2111, 2409
StuI AGGCCT 2 cut(s) 300, 745
StyD4I CCNGG 8 cut(s) 166, 506, 549, 550, 557, 922, 943, 1146
StyI CCWWGG 3 cut(s) 644, 1639, 1799
TaaI ACNGT 8 cut(s) 101, 290, 673, 861, 881, 1089, 1327, 1510
TaiI ACGT 5 cut(s) 123, 351, 794, 917, 1063
TaqI TCGA 6 cut(s) 15, 930, 1234, 1407, 1434, 1833
TatI WGTACW 2 cut(s) 566, 2462
Tru1I TTAA 6 cut(s) 1454, 1656, 1704, 1791, 1814, 2345
Tru9I TTAA 6 cut(s) 1454, 1656, 1704, 1791, 1814, 2345
TscAI CASTG 3 cut(s) 474, 866, 1094
TseFI GTSAC 4 cut(s) 881, 1168, 2040, 2455
TseI GCWGC 1 cut(s) 734
Tsp45I GTSAC 4 cut(s) 881, 1168, 2040, 2455
TspGWI ACGGA 3 cut(s) 285, 870, 1561
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 3 cut(s) 474, 866, 1094
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 4 cut(s) 319, 339, 504, 866
XapI RAATTY 4 cut(s) 172, 818, 2167, 2300
XceI RCATGY 4 cut(s) 972, 999, 2183, 2463
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 1 cut(s) 1052
XmnI GAANNNNTTC 2 cut(s) 1202, 1668
XspI CTAG 9 cut(s) 60, 132, 152, 837, 1635, 1854, 1974, 2111, 2409
Zsp2I ATGCAT 1 cut(s) 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.