Prupe.4G031600_v2.0.a1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000099
Physical Location & Seq
Reverse (-)
1467905 .. 1471535
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G031600.1

Sequence Viewer

Length: 2463 bp
ATGTTCATGAATTTTACGAAAGGTTTAATCGTTACAGCACTTGTTCTATTCGTTCTTCTTATCCGATGCTGTAATTCGATAGATAGCATAACAACTGATCAAGCCATTAGAGATGGTGAAGTTCTAGTCTCTAATGGAGAAGTGTTTGAGCTTGGTTTCTTCAGCCCTGGAAAATCCACCAACCGCTATGTTGGAATCTGGTACAAGAGAGATAAAGAAAAAACAGTTGTATGGGTTGCAAACAGAGACGATCCTATCAATGAAACCTCAGGGGTCCTGTCAATTGGTGCACATGACCTCATCCTCTACGCCAGGAACCAAAGCAACATTACTTTCTGGTCCACCAAATCCAATGTTTCAATCTCATCATCATCCACACCTGATCCTAAATACAAAGCTCAGCTCTTAGATACTGGAAACCTTGTTTTGGTTGAACGAGAGAGCCAAAAGGTAACTTGGCAAAGCTTTGATTATCTCACTCATACAGTGCTTCCATTCATGAAACTTGGAATAAACAAGCGGACAGGATTCAACTGGTTCCTAACTTCCTGGAAATCCGAAGATGACCCGGGAACCGGGAGTTACACCTATAAGATTGATCCAAATGGAGCTCCTCAGATGGTCTTGTACAAAGATAATGTTCGATACTGGAGGACTGGTAGGTGGTGGTGGAGTGACATACCGGTGGTGCTAACTACTTCTTTCTCCAATTCTAGCTTTTTGAACAATCAAGATGAGGTTACTGTTACTTGGGGTCTTCTCAACTCATCCATCTCCACAAGAATTGTGGTAGACTATTCTGGAACGATCCAACAATTTAACTGGTATGAACAAGACCTTGGATGGAATCAAATCTGGTCTGGCCCCATGGACAGATGTGATTATTATGGCAGGTGTGGAGCGTTTGGTTACTGTGACTCGGACAGTATCAGCGTGTTTGAATGCAAATGCCTACCAGGGTTCCAACCCAAGTTGCCAGACGAATGGAACATGAGAAATGCATCTAGTGGCTGCGTGAGGAGGCTCGAGTCGTGCAAGAACGGGGAAGGTTTCATCAAGTTGGTAAAAGTCAAGGTCCCTGATACATCGTGGGCACGGGTGGATATGAATTTGAGTTCGAAAGCATGTGAGCAGCAGTGCTTGAGGGATTGCTCTTGTTTGGCATACACCATCAGCAGCAATGCACCTATAATAGGGTGCATGACATGGTATGGGAAGTTAGTAGATACAAAGAATTTTGCAGATGGGGATCAAGACTTATACCTACGTGTAGATGCACTTGAGTTAGCTGAGTACACAAAGAAGTCCAAGGGTTTTCTTGCCAAAAAAGGGAGGCTGCCAATTTTGGTAGTGTCTATTGCTATGATATCCTTCTTCATTTGCTTTGGATGTTGGTGTGCAAAGAGGAAGAGAAAAAAATATCCCTGGGAAAGGAAGAATGATGATGACAGTAGAACACATCAAGACCTGACACTTTTTAACCTAAAAAGCATAGTTGCTGCTACAAACAATTTCTCTGCTGCTAACAAGCTGGGCGAAGGTGGATTTGGCCCAGTCTATAAGGGTCTATTAGCTAATGGACAAGAGGTGGCTGTGAAAAGACTATCAAAGAATTCGGGGCAAGGTCTAGAACAATTTAAGAATGAGGTTATGTTGATAGCAAAACTTCAGCACAGGAACCTGGTGAGGCTTTTTGGTTGCTGCATTCATACAAAAGAGAAGATGTTGATTTATGAATACTTGACAAACAAAAGCTTGGACTTCTTCATATTTGATAAAAAGAGAAGCTCGTTGTTAGACTGGAAAACACGTTTTGAAATTATATTTGGAATCGCTCGAGGTGTCTTATATCTTCATCAAGACTCGAGACTAAAAATTATCCACAGGGATTTAAAAGCTAGCAATGTCCTCTTGGATTCTACTATGAACCCAAAGATCTCAGATTTTGGAATGGCTAAAATGTTTGGCGAAGACCAAATGCAAGCAAAAACCAACCGAGTGGTTGGAACATATGGATACATGTCACCAGAATATGCAATGGAAGGGCGATATTCAGAAAAATCAGATGTTTTTAGCTTTGGTGTGTTACTGCTAGAGATCATTAGTGGCAAAAGGAACACTAGTTATGATAACCAAACTCCCTCCCCAAATTTGATTGGACAAATTTGGGATATGTGGACAGAGGAGCAGGCATTAGGTATGGTTGATCCATCCTTGGGGGAGTCATATCCTGCTCATGAGGTTTCAAGATGCATCCAGATTGGGCTACTTTGCGTGCAAGAAAGTGCATCAGATAGGCCAACCATGTCGGAAGTTATTTTTATGTTGGGTAATGAAACAACTCTTCCCTCTCCCAAGAAACCTGCATTTATATTACAATCCAGCAATCCAAACTCAATAGCATCAAAAGGATCAACTCCTTCTCTAAATGACGTAACGATAACTATGCTCGAAGCACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

821

Amino Acids

92.79

Weight (kDa)

8.46

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 882
Acc36I ACCTGC 2 cut(s) 882, 2371
AccI GTMKAC 1 cut(s) 792
AciI CCGC 2 cut(s) 184, 520
AclWI GGATC 7 cut(s) 245, 377, 593, 802, 1257, 2201, 2419
AcsI RAATTY 6 cut(s) 10, 1108, 1234, 1612, 2149, 2163
AcuI CTGAAG 2 cut(s) 145, 1652
AfaI GTAC 3 cut(s) 203, 629, 1295
AfiI CCNNNNNNNGG 6 cut(s) 427, 575, 1193, 1329, 1431, 2216
AflIII ACRYGT 3 cut(s) 1267, 1808, 2019
AgeI ACCGGT 1 cut(s) 682
AgsI TTSAA 7 cut(s) 360, 434, 532, 724, 941, 1817, 2247
AhlI ACTAGT 1 cut(s) 2120
AjnI CCWGG 6 cut(s) 166, 311, 548, 955, 1424, 1680
AjuI GAANNNNNNNTTGG 6 cut(s) 822, 854, 1530, 1562, 1809, 1841
Alw21I GWGCWC 2 cut(s) 292, 613
Alw26I GTCTC 3 cut(s) 133, 240, 1861
Alw44I GTGCAC 1 cut(s) 288
AlwI GGATC 7 cut(s) 245, 377, 593, 802, 1257, 2201, 2419
Ama87I CYCGRG 4 cut(s) 568, 1025, 1836, 1864
AoxI GGCC 3 cut(s) 862, 1549, 2297
ApaLI GTGCAC 1 cut(s) 288
ApeKI GCWGC 7 cut(s) 1011, 1132, 1176, 1336, 1499, 1520, 1701
ApoI RAATTY 6 cut(s) 10, 1108, 1234, 1612, 2149, 2163
AsiGI ACCGGT 1 cut(s) 682
AspS9I GGNCC 5 cut(s) 274, 339, 863, 1075, 1550
AsuC2I CCSGG 3 cut(s) 569, 570, 577
AsuHPI GGTGA 3 cut(s) 128, 1696, 2016
AsuII TTCGAA 1 cut(s) 1118
AsuNHI GCTAGC 1 cut(s) 1898
AvaI CYCGRG 4 cut(s) 568, 1025, 1836, 1864
AvaII GGWCC 3 cut(s) 274, 339, 1075
AxyI CCTNAGG 1 cut(s) 268
BaeGI GKGCMC 2 cut(s) 292, 1096
BanII GRGCYC 1 cut(s) 613
BbsI GAAGAC 2 cut(s) 749, 1977
Bbv12I GWGCWC 2 cut(s) 292, 613
BbvI GCAGC 7 cut(s) 998, 1144, 1188, 1323, 1486, 1507, 1688
BccI CCATC 7 cut(s) 107, 613, 779, 837, 1178, 1238, 2218
BcgI CGANNNNNNTGC 2 cut(s) 2428, 2462
BciT130I CCWGG 6 cut(s) 168, 313, 550, 957, 1426, 1682
BciVI GTATCC 1 cut(s) 2009
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 3 cut(s) 569, 570, 577
BcoDI GTCTC 3 cut(s) 133, 240, 1861
BcuI ACTAGT 1 cut(s) 2120
BfaI CTAG 7 cut(s) 125, 714, 1005, 1628, 1899, 2093, 2121
BfuAI ACCTGC 2 cut(s) 882, 2371
BfuI GTATCC 1 cut(s) 2009
BglII AGATCT 1 cut(s) 1935
BisI GCNGC 7 cut(s) 1012, 1133, 1177, 1337, 1500, 1521, 1702
BlpI GCTNAGC 1 cut(s) 399
BlsI GCNGC 7 cut(s) 1013, 1134, 1178, 1338, 1501, 1522, 1703
Bme1390I CCNGG 9 cut(s) 168, 313, 550, 569, 570, 577, 957, 1426, 1682
Bme18I GGWCC 3 cut(s) 274, 339, 1075
BmeT110I CYCGRG 4 cut(s) 568, 1025, 1836, 1864
BmgT120I GGNCC 5 cut(s) 274, 339, 863, 1075, 1550
BmiI GGNNCC 8 cut(s) 275, 317, 539, 574, 865, 962, 1077, 1679
BmrFI CCNGG 9 cut(s) 168, 313, 550, 569, 570, 577, 957, 1426, 1682
BmrI ACTGGG 1 cut(s) 1547
BmsI GCATC 7 cut(s) 56, 1010, 1264, 2240, 2262, 2297, 2411
BmtI GCTAGC 1 cut(s) 1902
BmuI ACTGGG 1 cut(s) 1547
BpiI GAAGAC 2 cut(s) 749, 1977
BpmI CTGGAG 1 cut(s) 670
Bpu1102I GCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 1118
BpuEI CTTGAG 2 cut(s) 1162, 1301
BpuMI CCSGG 3 cut(s) 569, 570, 577
BsaAI YACGTR 1 cut(s) 1268
BsaBI GATNNNNATC 1 cut(s) 1248
BsaJI CCNNGG 9 cut(s) 166, 568, 838, 867, 956, 1308, 1424, 1425, 2214
BsaWI WCCGGW 1 cut(s) 682
BsaXI ACNNNNNCTCC 2 cut(s) 643, 673
Bsc4I CCNNNNNNNGG 6 cut(s) 427, 575, 1193, 1329, 1431, 2216
Bse118I RCCGGY 1 cut(s) 682
Bse1I ACTGG 7 cut(s) 418, 539, 653, 661, 827, 1553, 1805
Bse21I CCTNAGG 1 cut(s) 268
Bse3DI GCAATG 3 cut(s) 1186, 1909, 2043
Bse8I GATNNNNATC 1 cut(s) 1248
BseBI CCWGG 6 cut(s) 168, 313, 550, 957, 1426, 1682
BseDI CCNNGG 9 cut(s) 166, 568, 838, 867, 956, 1308, 1424, 1425, 2214
BseGI GGATG 7 cut(s) 300, 371, 767, 848, 1394, 2210, 2253
BseJI GATNNNNATC 1 cut(s) 1248
BseLI CCNNNNNNNGG 6 cut(s) 427, 575, 1193, 1329, 1431, 2216
BseMI GCAATG 3 cut(s) 1186, 1909, 2043
BseMII CTCAG 5 cut(s) 282, 413, 629, 1281, 1953
BseNI ACTGG 7 cut(s) 418, 539, 653, 661, 827, 1553, 1805
BseRI GAGGAG 3 cut(s) 603, 1033, 2198
BseSI GKGCMC 2 cut(s) 292, 1096
BseXI GCAGC 7 cut(s) 998, 1144, 1188, 1323, 1486, 1507, 1688
BseYI CCCAGC 1 cut(s) 1531
BshFI GGCC 3 cut(s) 864, 1551, 2299
BshTI ACCGGT 1 cut(s) 682
BsiHKAI GWGCWC 2 cut(s) 292, 613
BsiHKCI CYCGRG 4 cut(s) 568, 1025, 1836, 1864
BsiSI CCGG 3 cut(s) 569, 576, 683
BslFI GGGAC 1 cut(s) 1061
BslI CCNNNNNNNGG 6 cut(s) 427, 575, 1193, 1329, 1431, 2216
BsmAI GTCTC 3 cut(s) 133, 240, 1861
BsmBI CGTCTC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 1061
BsmI GAATGC 2 cut(s) 947, 1704
BsnI GGCC 3 cut(s) 864, 1551, 2299
BsoBI CYCGRG 4 cut(s) 568, 1025, 1836, 1864
Bsp119I TTCGAA 1 cut(s) 1118
Bsp1286I GDGCHC 3 cut(s) 292, 613, 1096
Bsp1407I TGTACA 1 cut(s) 627
Bsp1720I GCTNAGC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 2 cut(s) 184, 520
BspANI GGCC 3 cut(s) 864, 1551, 2299
BspCNI CTCAG 5 cut(s) 281, 412, 628, 1282, 1952
BspHI TCATGA 3 cut(s) 6, 498, 2236
BspLI GGNNCC 8 cut(s) 275, 317, 539, 574, 865, 962, 1077, 1679
BspMI ACCTGC 2 cut(s) 882, 2371
BspOI GCTAGC 1 cut(s) 1902
BspPI GGATC 7 cut(s) 245, 377, 593, 802, 1257, 2201, 2419
BspT104I TTCGAA 1 cut(s) 1118
BsrDI GCAATG 3 cut(s) 1186, 1909, 2043
BsrFI RCCGGY 1 cut(s) 682
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 7 cut(s) 418, 539, 653, 661, 827, 1553, 1805
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 9 cut(s) 166, 568, 838, 867, 956, 1308, 1424, 1425, 2214
BssT1I CCWWGG 4 cut(s) 838, 867, 1308, 2214
Bst2UI CCWGG 6 cut(s) 168, 313, 550, 957, 1426, 1682
Bst4CI ACNGT 6 cut(s) 226, 487, 745, 914, 926, 1451
Bst6I CTCTTC 2 cut(s) 1403, 2349
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 1268
BstBI TTCGAA 1 cut(s) 1118
BstC8I GCNNGC 5 cut(s) 1900, 1983, 2190, 2276, 2458
BstDEI CTNAG 6 cut(s) 268, 399, 406, 615, 1290, 1939
BstDSI CCRYGG 1 cut(s) 867
BstENI CCTNNNNNAGG 2 cut(s) 1191, 1429
BstF5I GGATG 7 cut(s) 300, 371, 767, 848, 1394, 2210, 2253
BstMAI GTCTC 3 cut(s) 133, 240, 1861
BstNI CCWGG 6 cut(s) 168, 313, 550, 957, 1426, 1682
BstNSI RCATGY 2 cut(s) 1128, 2023
BstSCI CCNGG 9 cut(s) 166, 311, 548, 567, 568, 575, 955, 1424, 1680
BstSLI GKGCMC 2 cut(s) 292, 1096
BstV1I GCAGC 7 cut(s) 998, 1144, 1188, 1323, 1486, 1507, 1688
BstV2I GAAGAC 2 cut(s) 749, 1977
BstX2I RGATCY 1 cut(s) 1935
BstXI CCANNNNNNTGG 2 cut(s) 984, 1999
BstYI RGATCY 1 cut(s) 1935
Bsu36I CCTNAGG 1 cut(s) 268
BsuI GTATCC 1 cut(s) 2009
BsuRI GGCC 3 cut(s) 864, 1551, 2299
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 7 cut(s) 300, 371, 767, 848, 1394, 2210, 2253
BtsI GCAGTG 1 cut(s) 1142
BtsIMutI CAGTG 2 cut(s) 492, 1142
BveI ACCTGC 2 cut(s) 882, 2371
Cac8I GCNNGC 5 cut(s) 1900, 1983, 2190, 2276, 2458
CciI TCATGA 3 cut(s) 6, 498, 2236
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 5 cut(s) 274, 339, 863, 1075, 1550
Cfr9I CCCGGG 1 cut(s) 568
CsiI ACCWGGT 1 cut(s) 1680
Csp6I GTAC 3 cut(s) 202, 628, 1294
CspAI ACCGGT 1 cut(s) 682
CspCI CAANNNNNGTGG 4 cut(s) 766, 768, 801, 803
CviQI GTAC 3 cut(s) 202, 628, 1294
DdeI CTNAG 6 cut(s) 268, 399, 406, 615, 1290, 1939
DraI TTTAAA 1 cut(s) 1893
Eam1104I CTCTTC 2 cut(s) 1403, 2349
EarI CTCTTC 2 cut(s) 1403, 2349
Ecl136II GAGCTC 1 cut(s) 611
Eco130I CCWWGG 4 cut(s) 838, 867, 1308, 2214
Eco24I GRGCYC 1 cut(s) 613
Eco32I GATATC 1 cut(s) 1368
Eco47I GGWCC 3 cut(s) 274, 339, 1075
Eco53kI GAGCTC 1 cut(s) 611
Eco57I CTGAAG 2 cut(s) 145, 1652
Eco81I CCTNAGG 1 cut(s) 268
Eco88I CYCGRG 4 cut(s) 568, 1025, 1836, 1864
EcoICRI GAGCTC 1 cut(s) 611
EcoNI CCTNNNNNAGG 2 cut(s) 1191, 1429
EcoO109I RGGNCCY 2 cut(s) 274, 1075
EcoRI GAATTC 1 cut(s) 1612
EcoRII CCWGG 6 cut(s) 166, 311, 548, 955, 1424, 1680
EcoRV GATATC 1 cut(s) 1368
EcoT14I CCWWGG 4 cut(s) 838, 867, 1308, 2214
EcoT22I ATGCAT 2 cut(s) 1003, 2255
EcoT38I GRGCYC 1 cut(s) 613
ErhI CCWWGG 4 cut(s) 838, 867, 1308, 2214
Esp3I CGTCTC 1 cut(s) 240
FaqI GGGAC 1 cut(s) 1061
FauNDI CATATG 1 cut(s) 2011
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 7 cut(s) 1012, 1133, 1177, 1337, 1500, 1521, 1702
FokI GGATG 7 cut(s) 287, 358, 754, 855, 1401, 2197, 2240
FriOI GRGCYC 1 cut(s) 613
Fsp4HI GCNGC 7 cut(s) 1012, 1133, 1177, 1337, 1500, 1521, 1702
FspBI CTAG 7 cut(s) 125, 714, 1005, 1628, 1899, 2093, 2121
GluI GCNGC 7 cut(s) 1012, 1133, 1177, 1337, 1500, 1521, 1702
GsaI CCCAGC 1 cut(s) 1535
GsuI CTGGAG 1 cut(s) 670
HaeIII GGCC 3 cut(s) 864, 1551, 2299
HapII CCGG 3 cut(s) 569, 576, 683
HindIII AAGCTT 2 cut(s) 463, 1753
HinfI GANTC 9 cut(s) 195, 528, 847, 917, 1028, 1830, 1862, 1916, 2222
HpaII CCGG 3 cut(s) 569, 576, 683
HphI GGTGA 3 cut(s) 128, 1696, 2016
Hpy166II GTNNAC 5 cut(s) 290, 342, 793, 1296, 2178
Hpy188I TCNGA 9 cut(s) 65, 559, 618, 922, 1942, 2056, 2065, 2293, 2311
Hpy8I GTNNAC 5 cut(s) 290, 342, 793, 1296, 2178
HpyAV CCTTC 5 cut(s) 1040, 1381, 1532, 2036, 2430
HpyCH4III ACNGT 6 cut(s) 226, 487, 745, 914, 926, 1451
HpyCH4IV ACGT 3 cut(s) 1267, 1810, 2433
HpyF3I CTNAG 6 cut(s) 268, 399, 406, 615, 1290, 1939
HpySE526I ACGT 3 cut(s) 1267, 1810, 2433
Ksp22I TGATCA 1 cut(s) 97
LmnI GCTCC 4 cut(s) 608, 616, 899, 2185
Lsp1109I GCAGC 7 cut(s) 998, 1144, 1188, 1323, 1486, 1507, 1688
LweI GCATC 7 cut(s) 56, 1010, 1264, 2240, 2262, 2297, 2411
MabI ACCWGGT 1 cut(s) 1680
MaeI CTAG 7 cut(s) 125, 714, 1005, 1628, 1899, 2093, 2121
MaeII ACGT 3 cut(s) 1267, 1810, 2433
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 1935
MhlI GDGCHC 3 cut(s) 292, 613, 1096
MlyI GAGTC 4 cut(s) 911, 1037, 1856, 2231
MmeI TCCRAC 5 cut(s) 172, 835, 988, 1984, 2289
Mph1103I ATGCAT 2 cut(s) 1003, 2255
MseI TTAA 5 cut(s) 26, 819, 1479, 1638, 1892
MslI CAYNNNNRTG 1 cut(s) 683
MspI CCGG 3 cut(s) 569, 576, 683
MspR9I CCNGG 9 cut(s) 168, 313, 550, 569, 570, 577, 957, 1426, 1682
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 2 cut(s) 947, 1704
MvaI CCWGG 6 cut(s) 168, 313, 550, 957, 1426, 1682
NciI CCSGG 3 cut(s) 569, 570, 577
NcoI CCATGG 1 cut(s) 867
NdeI CATATG 1 cut(s) 2011
NheI GCTAGC 1 cut(s) 1898
NlaIV GGNNCC 8 cut(s) 275, 317, 539, 574, 865, 962, 1077, 1679
NmuCI GTSAC 3 cut(s) 674, 914, 2022
NsiI ATGCAT 2 cut(s) 1003, 2255
NspI RCATGY 2 cut(s) 1128, 2023
NspV TTCGAA 1 cut(s) 1118
PaeR7I CTCGAG 3 cut(s) 1025, 1836, 1864
PagI TCATGA 3 cut(s) 6, 498, 2236
PaqCI CACCTGC 1 cut(s) 882
PasI CCCWGGG 1 cut(s) 1425
PciI ACATGT 1 cut(s) 2019
PctI GAATGC 2 cut(s) 947, 1704
PfeI GAWTC 5 cut(s) 195, 528, 847, 1830, 1916
PfoI TCCNGGA 1 cut(s) 548
PinAI ACCGGT 1 cut(s) 682
PkrI GCNGC 7 cut(s) 1013, 1134, 1178, 1338, 1501, 1522, 1703
PleI GAGTC 4 cut(s) 911, 1036, 1856, 2230
PpsI GAGTC 4 cut(s) 911, 1036, 1856, 2230
Ppu21I YACGTR 1 cut(s) 1268
PpuMI RGGWCCY 2 cut(s) 274, 1075
PscI ACATGT 1 cut(s) 2019
Psp124BI GAGCTC 1 cut(s) 613
Psp5II RGGWCCY 2 cut(s) 274, 1075
Psp6I CCWGG 6 cut(s) 166, 311, 548, 955, 1424, 1680
PspFI CCCAGC 1 cut(s) 1531
PspGI CCWGG 6 cut(s) 166, 311, 548, 955, 1424, 1680
PspN4I GGNNCC 8 cut(s) 275, 317, 539, 574, 865, 962, 1077, 1679
PspPI GGNCC 5 cut(s) 274, 339, 863, 1075, 1550
PspPPI RGGWCCY 2 cut(s) 274, 1075
PspXI VCTCGAGB 2 cut(s) 1025, 1836
PsuI RGATCY 1 cut(s) 1935
RsaI GTAC 3 cut(s) 203, 629, 1295
RsaNI GTAC 3 cut(s) 202, 628, 1294
RseI CAYNNNNRTG 1 cut(s) 683
SacI GAGCTC 1 cut(s) 613
SaqAI TTAA 5 cut(s) 26, 819, 1479, 1638, 1892
SatI GCNGC 7 cut(s) 1012, 1133, 1177, 1337, 1500, 1521, 1702
Sau96I GGNCC 5 cut(s) 274, 339, 863, 1075, 1550
SchI GAGTC 4 cut(s) 911, 1037, 1856, 2231
ScrFI CCNGG 9 cut(s) 168, 313, 550, 569, 570, 577, 957, 1426, 1682
SduI GDGCHC 3 cut(s) 292, 613, 1096
SexAI ACCWGGT 1 cut(s) 1680
SfaNI GCATC 7 cut(s) 56, 1010, 1264, 2240, 2262, 2297, 2411
Sfr274I CTCGAG 3 cut(s) 1025, 1836, 1864
SfuI TTCGAA 1 cut(s) 1118
SinI GGWCC 3 cut(s) 274, 339, 1075
SlaI CTCGAG 3 cut(s) 1025, 1836, 1864
SmaI CCCGGG 1 cut(s) 570
SmiMI CAYNNNNRTG 1 cut(s) 683
SmlI CTYRAG 5 cut(s) 1025, 1141, 1280, 1836, 1864
SmoI CTYRAG 5 cut(s) 1025, 1141, 1280, 1836, 1864
SpeI ACTAGT 1 cut(s) 2120
SsiI CCGC 2 cut(s) 184, 520
SspMI CTAG 7 cut(s) 125, 714, 1005, 1628, 1899, 2093, 2121
SstI GAGCTC 1 cut(s) 613
StyD4I CCNGG 9 cut(s) 166, 311, 548, 567, 568, 575, 955, 1424, 1680
StyI CCWWGG 4 cut(s) 838, 867, 1308, 2214
TaaI ACNGT 6 cut(s) 226, 487, 745, 914, 926, 1451
TaiI ACGT 3 cut(s) 1270, 1813, 2436
TaqI TCGA 7 cut(s) 77, 643, 1026, 1118, 1837, 1865, 2451
TatI WGTACW 2 cut(s) 627, 1293
TfiI GAWTC 5 cut(s) 195, 528, 847, 1830, 1916
Tru1I TTAA 5 cut(s) 26, 819, 1479, 1638, 1892
Tru9I TTAA 5 cut(s) 26, 819, 1479, 1638, 1892
TscAI CASTG 2 cut(s) 492, 1142
TseFI GTSAC 3 cut(s) 674, 914, 2022
TseI GCWGC 7 cut(s) 1011, 1132, 1176, 1336, 1499, 1520, 1701
Tsp45I GTSAC 3 cut(s) 674, 914, 2022
TspMI CCCGGG 1 cut(s) 568
TspRI CASTG 2 cut(s) 492, 1142
VneI GTGCAC 1 cut(s) 288
VpaK11BI GGWCC 3 cut(s) 274, 339, 1075
XagI CCTNNNNNAGG 2 cut(s) 1191, 1429
XapI RAATTY 6 cut(s) 10, 1108, 1234, 1612, 2149, 2163
XbaI TCTAGA 1 cut(s) 1627
XceI RCATGY 2 cut(s) 1128, 2023
XcmI CCANNNNNNNNNTGG 1 cut(s) 784
XhoI CTCGAG 3 cut(s) 1025, 1836, 1864
XmaI CCCGGG 1 cut(s) 568
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 7 cut(s) 125, 714, 1005, 1628, 1899, 2093, 2121
Zsp2I ATGCAT 2 cut(s) 1003, 2255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.