Rw1G002050

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000099
Physical Location & Seq
Forward (+)
3711487 .. 3715195
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002050.1

Sequence Viewer

Length: 2523 bp
ATGAGTTTTAGTAGCGAAGCGTTTATCCAGATATCGCTTATCTTCTTTCTTCTCCCCTATAGCATTTGCCAGCTTCCTCTTGACGCCCTTACGCCAAACCAACCCATCAGAGACGGCGACGTTTTAGTCTCTAGTAGGAAAATCTTTGCACTAGGGTTCTTCAGCCCCGGAAATTCTGTGCACCGCTACGTTGGAGTTTGGTTCAACAATGTTCCAACCCAAACCATCGTCTGGGTTGCAAACAGAGACAACCCGGTCGATGATTCTTCTGGAGTGCTATCGATCAGTGGTGATGGAGGCCTTGTCATACACGGAAAGGACCGAAGTATCCCTCTTTGGTCAGCAAACGTTACTCTCTCTTCTCCAAACAATTCCATAGCCAAGCTTTTGGATACAGGAAATCTTGTTTTGCTTGAGAGAGAGAGTGGCCAAAGGGTTTTGTGGCAAGGCTTTGATTATCCCTCAGACACAGTACTTTCCTCTATGAAGCTGGGCTTGAACCGGCGGTCCGGGTTGGACCGGTTCCTAACATCATGGAAGTCAAAAGATGACCCGGGAACCGGGAGCTGTACATTTGAGATAGACCCAAGGGGGATTCCGCAGTTGTATACGTACAAAGGTGGACGCCCATTCTGGCGGGGTGGCCCTTGGATGGGGGAGAGATGGAGTGGTCTACCCGACCTTACAACTACCGTTGGAGTCTACAATATAAGTTTTGTCAAAAATGAAGACGAGATATCCTTCGTGTATGCTGTTGCCAATGACTCATGGATCACAAGGGGGGTGGTAGATGAATCAGGAATATTTCAACGGTTCCAGTGGAACGATCTGGAAAACCAATGGATCAACTTGATCTCCTTCCCGAATGAGTGGTGTGATAACTACGGACCGTGCGGTCCAAATAGTAACTGTGACCCATACAAGGACTATAAGTTCGCTTGCACATGCCTACCCGGGTTTGAACCCAAATCGCCTGGTGACTGGTACCTGAGAATGGGGGCGGGAGGGTGCATTAGAAAAGCGGGAGCATCAGCGTGTCGGAATGGAGAAGGGTTCGTGAAGGTGGTTCGTGTAAAGGTGCCAGACACATCTACGGCACGTGTGAATATGAGTTTGAGTATGAAAGCTTGTGAGCTAGAGTGCTTGATGGATTGTTCTTGCACGGCTTACACGAGTGCAGATGAGCGGGGAGGAGGAATAGGGTGCATGACATGGCATGGGGACTTGATGGACACGATGACTTACTCCAATGTTGGTCAAGATTTATATGTTCGAATTGATGCAACTTCTTTAGCTCAATATGCAAATTCAAGTGGTTCTCTTAGCAAGAAGGCAACGGTGGCAATTTCAGTAGGATCTGTTGCGGTTTTTCTTCTCTTACTTACCCTTTGTTGGTTGGTAAGGATGAAGATGGAAGGTGAGCCTAGACGAAATAAATATTCATTTATACTTACCACCGGGTCAACCTACTTCGAAGAATCTTCTAGCGGAACAGCTCTTGATGATAATAGAATACACCCAGAGTTATCATTCTTTCATCTAAAAACCATAGCAACTGCCACAAACAATTTCTCCATTGAAAACAAGCTTGGAGAAGGAGGTTTTGGCTCTGTTTATAAGGGCATACTTTACGATGGAAAGGAAATAGCAGCAAAAAGACTATCCAAGTTTTCTGGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTAATTGCAAAACTCCAACACAAAAATCTTGTCAAAATTTTAGGTTGTTGTTTTGAAGATGAAGAGAAGATCCTAATCTATGAATACTTGCCGAACAAAAGTTTGGACTCTTTCATCTTTAATGAAACAAAGAGCGCACTTCTAAATTGGACAAAACGCTTCGAGATTATCTTAGGGATTGCTAGAGGGGTATTATATCTTCACGAAGATTCGAGATTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTTCTATTGGATAATTCTTTGAACCCAAAAATTGCAGATTTTGGTATGGCTAGAATATTTAGTGGGGAGCAAAGTAAAGCAAAAACCAATCGTGTGGTTGGAACATATGGTTACATGTCGCCGGAATATGCAATGCAAGGACTATTTTCAATAAAGTCTGATGTTTATAGTTTTGGTGTTTTACAACTAGAAATAATTACTGGCAAAAAGAATGTTGGTTACCATGAGGAGTATCCTGATTCAAATTTGGTTGGACATGTTTGGGAGTTGTGGAATGAAGGCAGAGCCGTGGAAATCATTGATTCATCTATAGGCGAATCTTACCTTGTTAGTGAAGTTATGAGGTGCATTCAAATAGCGCTCTTGTGTGTGCAAGAATTTGCAACTGACCGACCAACCATGTCGGCAGTTGTTTCCATGTTAGGTAATGACGTAGCTCTTCCTTCACCAAGACAACCGCCATTTTTACTGAAGAGAATGAGTCCTAGTGTGAGCCCATCCAGCTTCAAAGGAGCTAATTCAATAAATGATGTCACATGTACAATTGTAGAAGCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

840

Amino Acids

93.24

Weight (kDa)

5.86

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 180 8e-35 D-mannose binding lectin
S_locus_glycop PF00954 212 - 324 1.2e-25 S-locus glycoprotein domain
PAN_2 PF08276 346 - 412 1.4e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 525 - 795 2.4e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 525 - 791 3.6e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1617
AasI GACNNNNNNGTC 3 cut(s) 125, 254, 894
Acc65I GGTACC 1 cut(s) 984
AccB1I GGYRCC 2 cut(s) 984, 1078
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 1186
AccI GTMKAC 3 cut(s) 608, 673, 702
AclI AACGTT 1 cut(s) 348
AclWI GGATC 4 cut(s) 779, 851, 1363, 1774
AcoI YGGCCR 2 cut(s) 427, 1675
AcsI RAATTY 5 cut(s) 172, 1306, 1746, 2206, 2339
AcuI CTGAAG 2 cut(s) 145, 2453
AcvI CACGTG 1 cut(s) 1100
AcyI GRCGYC 2 cut(s) 84, 625
AfaI GTAC 5 cut(s) 474, 571, 614, 986, 2503
AfeI AGCGCT 1 cut(s) 2322
AfiI CCNNNNNNNGG 7 cut(s) 231, 560, 652, 653, 922, 994, 1392
AflIII ACRYGT 4 cut(s) 1099, 2076, 2218, 2498
AgeI ACCGGT 1 cut(s) 519
AjnI CCWGG 1 cut(s) 973
AjuI GAANNNNNNNTTGG 6 cut(s) 1572, 1587, 1604, 1619, 1796, 1828
AloI GAACNNNNNNTCC 4 cut(s) 491, 523, 917, 949
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 3 cut(s) 105, 133, 240
Alw44I GTGCAC 1 cut(s) 179
AlwI GGATC 4 cut(s) 779, 851, 1363, 1774
Ama87I CYCGRG 2 cut(s) 553, 953
Aor51HI AGCGCT 1 cut(s) 2322
AoxI GGCC 5 cut(s) 298, 427, 643, 1675, 1953
ApaLI GTGCAC 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 1649
ApoI RAATTY 5 cut(s) 172, 1306, 1746, 2206, 2339
ArsI GACNNNNNNTTYG 4 cut(s) 917, 949, 1725, 1757
AsiGI ACCGGT 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 1707
Asp718I GGTACC 1 cut(s) 984
AspLEI GCGC 2 cut(s) 1847, 2323
AspS9I GGNCC 6 cut(s) 319, 507, 517, 644, 887, 896
AsuC2I CCSGG 9 cut(s) 168, 254, 511, 554, 555, 562, 954, 955, 1459
AsuHPI GGTGA 4 cut(s) 302, 989, 1430, 2400
AsuII TTCGAA 2 cut(s) 1273, 1473
AvaI CYCGRG 2 cut(s) 553, 953
AvaII GGWCC 5 cut(s) 319, 507, 517, 887, 896
BaeGI GKGCMC 1 cut(s) 183
BaeI ACNNNNGTAYC 2 cut(s) 310, 343
BalI TGGCCA 2 cut(s) 429, 1677
BanI GGYRCC 2 cut(s) 984, 1078
BanII GRGCYC 1 cut(s) 2459
BarI GAAGNNNNNNTAC 2 cut(s) 1893, 1925
BauI CACGAG 1 cut(s) 1171
BbrPI CACGTG 1 cut(s) 1100
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 1 cut(s) 1661
BceAI ACGGC 4 cut(s) 130, 1110, 1179, 2234
BciT130I CCWGG 1 cut(s) 975
BciVI GTATCC 3 cut(s) 338, 385, 2205
BcnI CCSGG 9 cut(s) 168, 254, 511, 554, 555, 562, 954, 955, 1459
BcoDI GTCTC 3 cut(s) 105, 133, 240
BfaI CTAG 9 cut(s) 132, 152, 1136, 1425, 1485, 1893, 2013, 2150, 2448
BfmI CTRYAG 2 cut(s) 58, 2271
BfoI RGCGCY 1 cut(s) 2324
BfuI GTATCC 3 cut(s) 338, 385, 2205
BglII AGATCT 1 cut(s) 1945
BisI GCNGC 1 cut(s) 1650
BlsI GCNGC 1 cut(s) 1651
BmcAI AGTACT 1 cut(s) 474
Bme18I GGWCC 5 cut(s) 319, 507, 517, 887, 896
BmeT110I CYCGRG 2 cut(s) 553, 953
BmgT120I GGNCC 6 cut(s) 319, 507, 517, 644, 887, 896
BmiI GGNNCC 5 cut(s) 524, 559, 815, 986, 1080
BmsI GCATC 2 cut(s) 1037, 1270
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 291
Bpu14I TTCGAA 2 cut(s) 1273, 1473
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 9 cut(s) 168, 254, 511, 554, 555, 562, 954, 955, 1459
Bsa29I ATCGAT 1 cut(s) 281
BsaAI YACGTR 2 cut(s) 612, 1100
BsaBI GATNNNNATC 1 cut(s) 1784
BsaHI GRCGYC 2 cut(s) 84, 625
BsaJI CCNNGG 7 cut(s) 166, 553, 587, 647, 953, 1678, 2250
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 6 cut(s) 288, 318, 839, 869, 1556, 1586
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 560, 652, 653, 922, 994, 1392
Bse118I RCCGGY 2 cut(s) 501, 519
Bse1I ACTGG 3 cut(s) 817, 986, 2167
Bse3DI GCAATG 2 cut(s) 1966, 2100
Bse8I GATNNNNATC 1 cut(s) 1784
BseBI CCWGG 1 cut(s) 975
BseCI ATCGAT 1 cut(s) 281
BseDI CCNNGG 7 cut(s) 166, 553, 587, 647, 953, 1678, 2250
BseGI GGATG 3 cut(s) 657, 1410, 2459
BseJI GATNNNNATC 1 cut(s) 1784
BseLI CCNNNNNNNGG 7 cut(s) 231, 560, 652, 653, 922, 994, 1392
BseMI GCAATG 2 cut(s) 1966, 2100
BseMII CTCAG 2 cut(s) 477, 980
BseNI ACTGG 3 cut(s) 817, 986, 2167
BseRI GAGGAG 2 cut(s) 1206, 2204
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 1 cut(s) 1661
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 1197
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 5 cut(s) 300, 429, 645, 1677, 1955
BshNI GGYRCC 2 cut(s) 984, 1078
BshTI ACCGGT 1 cut(s) 519
BshVI ATCGAT 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 258
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 2 cut(s) 553, 953
BslFI GGGAC 1 cut(s) 1235
BslI CCNNNNNNNGG 7 cut(s) 231, 560, 652, 653, 922, 994, 1392
BsmAI GTCTC 3 cut(s) 105, 133, 240
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 1235
BsmI GAATGC 1 cut(s) 2310
BsnI GGCC 5 cut(s) 300, 429, 645, 1677, 1955
BsoBI CYCGRG 2 cut(s) 553, 953
Bsp119I TTCGAA 2 cut(s) 1273, 1473
Bsp1286I GDGCHC 2 cut(s) 183, 2459
Bsp1407I TGTACA 2 cut(s) 569, 2501
Bsp143I GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
BspANI GGCC 5 cut(s) 300, 429, 645, 1677, 1955
BspCNI CTCAG 2 cut(s) 476, 981
BspDI ATCGAT 1 cut(s) 281
BspLI GGNNCC 5 cut(s) 524, 559, 815, 986, 1080
BspPI GGATC 4 cut(s) 779, 851, 1363, 1774
BspQI GCTCTTC 1 cut(s) 2406
BspT104I TTCGAA 2 cut(s) 1273, 1473
BspT107I GGYRCC 2 cut(s) 984, 1078
BsrBI CCGCTC 1 cut(s) 1186
BsrDI GCAATG 2 cut(s) 1966, 2100
BsrFI RCCGGY 2 cut(s) 501, 519
BsrGI TGTACA 2 cut(s) 569, 2501
BsrI ACTGG 3 cut(s) 817, 986, 2167
BssAI RCCGGY 2 cut(s) 501, 519
BssECI CCNNGG 7 cut(s) 166, 553, 587, 647, 953, 1678, 2250
BssMI GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
BssNAI GTATAC 1 cut(s) 609
BssNI GRCGYC 2 cut(s) 84, 625
BssSI CACGAG 1 cut(s) 1171
BssT1I CCWWGG 3 cut(s) 587, 647, 1678
Bst1107I GTATAC 1 cut(s) 609
Bst2BI CACGAG 1 cut(s) 1171
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 6 cut(s) 472, 694, 813, 891, 911, 1339
Bst6I CTCTTC 5 cut(s) 364, 1683, 1767, 2406, 2429
BstACI GRCGYC 2 cut(s) 84, 625
BstAUI TGTACA 2 cut(s) 569, 2501
BstBAI YACGTR 2 cut(s) 612, 1100
BstBI TTCGAA 2 cut(s) 1273, 1473
BstC8I GCNNGC 4 cut(s) 71, 940, 1957, 2518
BstDEI CTNAG 4 cut(s) 463, 989, 1322, 1882
BstDSI CCRYGG 1 cut(s) 2250
BstEII GGTNACC 1 cut(s) 2180
BstF5I GGATG 3 cut(s) 657, 1410, 2459
BstH2I RGCGCY 1 cut(s) 2324
BstHHI GCGC 2 cut(s) 1847, 2323
BstKTI GATC 8 cut(s) 285, 774, 829, 846, 855, 1358, 1782, 1948
BstMAI GTCTC 3 cut(s) 105, 133, 240
BstMBI GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 3 cut(s) 1301, 1340, 2463
BstNI CCWGG 1 cut(s) 975
BstNSI RCATGY 4 cut(s) 948, 2080, 2222, 2502
BstPI GGTNACC 1 cut(s) 2180
BstSFI CTRYAG 2 cut(s) 58, 2271
BstSLI GKGCMC 1 cut(s) 183
BstSNI TACGTA 1 cut(s) 612
BstV1I GCAGC 1 cut(s) 1661
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 3 cut(s) 1355, 1779, 1945
BstXI CCANNNNNNTGG 2 cut(s) 388, 2056
BstYI RGATCY 3 cut(s) 1355, 1779, 1945
BstZ17I GTATAC 1 cut(s) 609
Bsu15I ATCGAT 1 cut(s) 281
BsuI GTATCC 3 cut(s) 338, 385, 2205
BsuRI GGCC 5 cut(s) 300, 429, 645, 1677, 1955
BsuTUI ATCGAT 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 2250
BtsCI GGATG 3 cut(s) 657, 1410, 2459
BtsIMutI CAGTG 2 cut(s) 292, 824
Cac8I GCNNGC 4 cut(s) 71, 940, 1957, 2518
CfoI GCGC 2 cut(s) 1847, 2323
Cfr10I RCCGGY 2 cut(s) 501, 519
Cfr13I GGNCC 6 cut(s) 319, 507, 517, 644, 887, 896
Cfr9I CCCGGG 2 cut(s) 553, 953
ClaI ATCGAT 1 cut(s) 281
CpoI CGGWCCG 2 cut(s) 507, 887
CseI GACGC 2 cut(s) 92, 633
Csp6I GTAC 5 cut(s) 473, 570, 613, 985, 2502
CspAI ACCGGT 1 cut(s) 519
CspCI CAANNNNNGTGG 2 cut(s) 765, 800
CspI CGGWCCG 2 cut(s) 507, 887
CviQI GTAC 5 cut(s) 473, 570, 613, 985, 2502
DdeI CTNAG 4 cut(s) 463, 989, 1322, 1882
DpnI GATC 8 cut(s) 284, 773, 828, 845, 854, 1357, 1781, 1947
DpnII GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
DrdI GACNNNNNNGTC 3 cut(s) 125, 254, 894
DseDI GACNNNNNNGTC 3 cut(s) 125, 254, 894
EaeI YGGCCR 2 cut(s) 427, 1675
Eam1104I CTCTTC 5 cut(s) 364, 1683, 1767, 2406, 2429
EarI CTCTTC 5 cut(s) 364, 1683, 1767, 2406, 2429
Eco105I TACGTA 1 cut(s) 612
Eco130I CCWWGG 3 cut(s) 587, 647, 1678
Eco147I AGGCCT 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 2459
Eco32I GATATC 2 cut(s) 33, 738
Eco47I GGWCC 5 cut(s) 319, 507, 517, 887, 896
Eco47III AGCGCT 1 cut(s) 2322
Eco57I CTGAAG 2 cut(s) 145, 2453
Eco72I CACGTG 1 cut(s) 1100
Eco88I CYCGRG 2 cut(s) 553, 953
Eco91I GGTNACC 1 cut(s) 2180
EcoO65I GGTNACC 1 cut(s) 2180
EcoRII CCWGG 1 cut(s) 973
EcoRV GATATC 2 cut(s) 33, 738
EcoT14I CCWWGG 3 cut(s) 587, 647, 1678
EcoT38I GRGCYC 1 cut(s) 2459
ErhI CCWWGG 3 cut(s) 587, 647, 1678
Esp3I CGTCTC 1 cut(s) 105
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 1 cut(s) 1235
FauI CCCGC 4 cut(s) 630, 994, 1015, 1179
FauNDI CATATG 1 cut(s) 2068
FblI GTMKAC 3 cut(s) 608, 673, 702
Fnu4HI GCNGC 1 cut(s) 1650
FokI GGATG 3 cut(s) 664, 1417, 2446
FriOI GRGCYC 1 cut(s) 2459
Fsp4HI GCNGC 1 cut(s) 1650
FspBI CTAG 9 cut(s) 132, 152, 1136, 1425, 1485, 1893, 2013, 2150, 2448
GlaI GCGC 2 cut(s) 1846, 2322
GluI GCNGC 1 cut(s) 1650
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 2324
HaeIII GGCC 5 cut(s) 300, 429, 645, 1677, 1955
HgaI GACGC 2 cut(s) 92, 633
HhaI GCGC 2 cut(s) 1847, 2323
Hin1I GRCGYC 2 cut(s) 84, 625
Hin6I GCGC 2 cut(s) 1845, 2321
HinP1I GCGC 2 cut(s) 1845, 2321
HincII GTYRAC 1 cut(s) 1464
HindII GTYRAC 1 cut(s) 1464
HindIII AAGCTT 3 cut(s) 383, 1125, 1586
HphI GGTGA 4 cut(s) 302, 989, 1430, 2400
Hpy166II GTNNAC 6 cut(s) 181, 609, 623, 674, 703, 1464
Hpy188I TCNGA 4 cut(s) 110, 466, 1041, 2122
Hpy8I GTNNAC 6 cut(s) 181, 609, 623, 674, 703, 1464
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 9 cut(s) 751, 868, 1043, 1054, 1324, 1409, 1589, 2234, 2415
HpyCH4III ACNGT 6 cut(s) 472, 694, 813, 891, 911, 1339
HpyCH4IV ACGT 6 cut(s) 120, 189, 348, 611, 1099, 2394
HpyF10VI GCNNNNNNNGC 3 cut(s) 1301, 1340, 2463
HpyF3I CTNAG 4 cut(s) 463, 989, 1322, 1882
HpySE526I ACGT 6 cut(s) 120, 189, 348, 611, 1099, 2394
Hsp92I GRCGYC 2 cut(s) 84, 625
HspAI GCGC 2 cut(s) 1845, 2321
KpnI GGTACC 1 cut(s) 988
Kzo9I GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
LguI GCTCTTC 1 cut(s) 2406
LmnI GCTCC 4 cut(s) 564, 1025, 2029, 2474
Lsp1109I GCAGC 1 cut(s) 1661
LweI GCATC 2 cut(s) 1037, 1270
MaeI CTAG 9 cut(s) 132, 152, 1136, 1425, 1485, 1893, 2013, 2150, 2448
MaeII ACGT 6 cut(s) 120, 189, 348, 611, 1099, 2394
MaeIII GTNAC 7 cut(s) 349, 905, 911, 977, 2072, 2180, 2494
MalI GATC 8 cut(s) 284, 773, 828, 845, 854, 1357, 1781, 1947
MbiI CCGCTC 1 cut(s) 1186
MboI GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
MfeI CAATTG 1 cut(s) 2505
MflI RGATCY 3 cut(s) 1355, 1779, 1945
MhlI GDGCHC 2 cut(s) 183, 2459
MlsI TGGCCA 2 cut(s) 429, 1677
MluNI TGGCCA 2 cut(s) 429, 1677
MlyI GAGTC 4 cut(s) 708, 758, 1811, 2452
MmeI TCCRAC 8 cut(s) 172, 239, 495, 676, 1019, 1750, 2041, 2194
Mox20I TGGCCA 2 cut(s) 429, 1677
MroXI GAANNNNTTC 1 cut(s) 1707
MscI TGGCCA 2 cut(s) 429, 1677
MseI TTAA 3 cut(s) 1695, 1830, 1928
MslI CAYNNNNRTG 1 cut(s) 1033
Msp20I TGGCCA 2 cut(s) 429, 1677
MunI CAATTG 1 cut(s) 2505
Mva1269I GAATGC 1 cut(s) 2310
MvaI CCWGG 1 cut(s) 975
MwoI GCNNNNNNNGC 3 cut(s) 1301, 1340, 2463
NciI CCSGG 9 cut(s) 168, 254, 511, 554, 555, 562, 954, 955, 1459
NdeI CATATG 1 cut(s) 2068
NdeII GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
NlaIV GGNNCC 5 cut(s) 524, 559, 815, 986, 1080
NmuCI GTSAC 3 cut(s) 911, 977, 2494
NspI RCATGY 4 cut(s) 948, 2080, 2222, 2502
NspV TTCGAA 2 cut(s) 1273, 1473
PceI AGGCCT 1 cut(s) 300
PciI ACATGT 3 cut(s) 2076, 2218, 2498
PciSI GCTCTTC 1 cut(s) 2406
PcsI WCGNNNNNNNCGW 1 cut(s) 1169
PctI GAATGC 1 cut(s) 2310
PdmI GAANNNNTTC 1 cut(s) 1707
PfeI GAWTC 8 cut(s) 263, 595, 794, 1478, 1919, 2201, 2264, 2279
PflMI CCANNNNNTGG 1 cut(s) 231
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 1 cut(s) 1651
PleI GAGTC 4 cut(s) 707, 758, 1811, 2451
PmaCI CACGTG 1 cut(s) 1100
PmlI CACGTG 1 cut(s) 1100
PpsI GAGTC 4 cut(s) 707, 758, 1811, 2451
Ppu21I YACGTR 2 cut(s) 612, 1100
PscI ACATGT 3 cut(s) 2076, 2218, 2498
PsiI TTATAA 1 cut(s) 1617
Psp1406I AACGTT 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 973
PspCI CACGTG 1 cut(s) 1100
PspEI GGTNACC 1 cut(s) 2180
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 5 cut(s) 524, 559, 815, 986, 1080
PspPI GGNCC 6 cut(s) 319, 507, 517, 644, 887, 896
PsuI RGATCY 3 cut(s) 1355, 1779, 1945
RsaI GTAC 5 cut(s) 474, 571, 614, 986, 2503
RsaNI GTAC 5 cut(s) 473, 570, 613, 985, 2502
RseI CAYNNNNRTG 1 cut(s) 1033
Rsr2I CGGWCCG 2 cut(s) 507, 887
RsrII CGGWCCG 2 cut(s) 507, 887
SapI GCTCTTC 1 cut(s) 2406
SaqAI TTAA 3 cut(s) 1695, 1830, 1928
SatI GCNGC 1 cut(s) 1650
Sau3AI GATC 8 cut(s) 282, 771, 826, 843, 852, 1355, 1779, 1945
Sau96I GGNCC 6 cut(s) 319, 507, 517, 644, 887, 896
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 4 cut(s) 708, 758, 1811, 2452
SduI GDGCHC 2 cut(s) 183, 2459
SfaNI GCATC 2 cut(s) 1037, 1270
SfcI CTRYAG 2 cut(s) 58, 2271
SfuI TTCGAA 2 cut(s) 1273, 1473
SinI GGWCC 5 cut(s) 319, 507, 517, 887, 896
SmaI CCCGGG 2 cut(s) 555, 955
SmiMI CAYNNNNRTG 1 cut(s) 1033
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SnaBI TACGTA 1 cut(s) 612
SseBI AGGCCT 1 cut(s) 300
SspI AATATT 3 cut(s) 804, 1439, 2019
SspMI CTAG 9 cut(s) 132, 152, 1136, 1425, 1485, 1893, 2013, 2150, 2448
StuI AGGCCT 1 cut(s) 300
StyI CCWWGG 3 cut(s) 587, 647, 1678
TaaI ACNGT 6 cut(s) 472, 694, 813, 891, 911, 1339
TaiI ACGT 6 cut(s) 123, 192, 351, 614, 1102, 2397
TaqI TCGA 6 cut(s) 258, 281, 1273, 1473, 1872, 1922
TaqII GACCGA 2 cut(s) 336, 2367
TatI WGTACW 3 cut(s) 472, 569, 2501
TfiI GAWTC 8 cut(s) 263, 595, 794, 1478, 1919, 2201, 2264, 2279
Tru1I TTAA 3 cut(s) 1695, 1830, 1928
Tru9I TTAA 3 cut(s) 1695, 1830, 1928
TscAI CASTG 2 cut(s) 292, 824
TseFI GTSAC 3 cut(s) 911, 977, 2494
TseI GCWGC 1 cut(s) 1649
Tsp45I GTSAC 3 cut(s) 911, 977, 2494
TspGWI ACGGA 2 cut(s) 327, 900
TspMI CCCGGG 2 cut(s) 553, 953
TspRI CASTG 2 cut(s) 292, 824
Van91I CCANNNNNTGG 1 cut(s) 231
VneI GTGCAC 1 cut(s) 179
VpaK11BI GGWCC 5 cut(s) 319, 507, 517, 887, 896
XapI RAATTY 5 cut(s) 172, 1306, 1746, 2206, 2339
XceI RCATGY 4 cut(s) 948, 2080, 2222, 2502
XmaI CCCGGG 2 cut(s) 553, 953
XmiI GTMKAC 3 cut(s) 608, 673, 702
XmnI GAANNNNTTC 1 cut(s) 1707
XspI CTAG 9 cut(s) 132, 152, 1136, 1425, 1485, 1893, 2013, 2150, 2448
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.