RchiOBHm_Chr5g0035661

alpha-1,4-glucosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000099
Physical Location & Seq
Reverse (-)
29673114 .. 29673790
677 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31450

Sequence Viewer

Length: 669 bp
ATGTATGTTTTGTCAACAGGGATCATAAGGGTGGTGATAGATGAATCAGGATCATTTCAGGTGTTCGGGTGGAATGATCAATGGGTCAAATTCTACTCCTACCCGACTGGGTGGTGTGATCCCTATGGACGGTGCGGTCCAAATACTAACTGTGACCCAGCTGCCAAGGACTTTAAGTCGGCTTGCACGTGCCTACCTGGGTTCGAATCCAAATCACCTAGTTTGAGTGTGGGTGAGGGTGAGGGTGAGGGTCGGTGCATTAGGAAAGTGGGAGCGTCCACATGTCAAAATGGAGAAGGGTTCGTGAAGGTGGCGCGTGTAAAATTACCAGACTCGTCTACAGCACGTGTGAACTTGAATATGAGTTTGGAAGAGTGCAAACAAAAGTGCTTGATGGCTTGTTCTTGCACAGCTTACACGAGTGCGGATGAGCGGGGAGGTGGGATTGGGTGTGTGACATGGCACGGGGACTTGATGGACACGAGCACTTTCTCCGATGTTGGTCAAAATTTATATGTTCGAGTCGATGCAACTTCTTTAGGTACGTTCTTTATGTTTATAATAGCTAGAGCTAAATGTGGTCGTCTAAACAAATATGGAAAAGTAGTGAATTCAGAGTATTTAACATCTAGGCTTGCTCACATAAGTGTTAATTTTAAGGGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_31

Protein Analysis

222

Amino Acids

24.19

Weight (kDa)

8.19

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 8 - 71 3.3e-11 S-locus glycoprotein domain
PAN_2 PF08276 95 - 161 5.7e-23 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AasI GACNNNNNNGTC 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 338
AccII CGCG 1 cut(s) 316
AciI CCGC 3 cut(s) 135, 425, 433
AclWI GGATC 3 cut(s) 29, 58, 113
AcsI RAATTY 3 cut(s) 89, 508, 610
AcvI CACGTG 2 cut(s) 189, 347
AfaI GTAC 1 cut(s) 544
AfiI CCNNNNNNNGG 1 cut(s) 129
AflIII ACRYGT 2 cut(s) 281, 346
AgsI TTSAA 1 cut(s) 358
AhdI GACNNNNNGTC 1 cut(s) 175
AjnI CCWGG 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AleI CACNNNNGTG 1 cut(s) 645
AluBI AGCT 4 cut(s) 161, 413, 566, 572
AluI AGCT 4 cut(s) 161, 413, 566, 572
Alw21I GWGCWC 1 cut(s) 488
AlwI GGATC 3 cut(s) 29, 58, 113
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 3 cut(s) 89, 508, 610
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 1 cut(s) 137
AsuHPI GGTGA 5 cut(s) 46, 207, 245, 251, 257
AsuII TTCGAA 1 cut(s) 204
AvaII GGWCC 1 cut(s) 137
BauI CACGAG 2 cut(s) 418, 481
BbrPI CACGTG 2 cut(s) 189, 347
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 388, 469
BciT130I CCWGG 1 cut(s) 198
BclI TGATCA 1 cut(s) 76
BfaI CTAG 3 cut(s) 219, 567, 630
BfmI CTRYAG 1 cut(s) 339
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 198
Bme18I GGWCC 1 cut(s) 137
BmeRI GACNNNNNGTC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 517
BmuI ACTGGG 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 189, 347
BsaJI CCNNGG 2 cut(s) 165, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 165, 197
BseGI GGATG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 148
BseYI CCCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 316
BsiHKAI GWGCWC 1 cut(s) 488
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 482
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 4 cut(s) 21, 50, 76, 118
BspACI CCGC 3 cut(s) 135, 425, 433
BspFNI CGCG 1 cut(s) 316
BspPI GGATC 3 cut(s) 29, 58, 113
BspT104I TTCGAA 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 433
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 165, 197
BssMI GATC 4 cut(s) 21, 50, 76, 118
BssSI CACGAG 2 cut(s) 418, 481
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 2 cut(s) 418, 481
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 2 cut(s) 132, 152
Bst6I CTCTTC 1 cut(s) 366
BstBAI YACGTR 2 cut(s) 189, 347
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 184, 636
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 1 cut(s) 316
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 4 cut(s) 24, 53, 79, 121
BstMBI GATC 4 cut(s) 21, 50, 76, 118
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 339
BstUI CGCG 1 cut(s) 316
BstV1I GCAGC 1 cut(s) 148
BtsCI GGATG 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 184, 636
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 137
CseI GACGC 1 cut(s) 264
Csp6I GTAC 1 cut(s) 543
CviAII CATG 2 cut(s) 282, 459
CviJI RGCY 7 cut(s) 161, 182, 398, 413, 566, 572, 634
CviKI_1 RGCY 7 cut(s) 161, 182, 398, 413, 566, 572, 634
CviQI GTAC 1 cut(s) 543
DpnI GATC 4 cut(s) 23, 52, 78, 120
DpnII GATC 4 cut(s) 21, 50, 76, 118
DrdI GACNNNNNNGTC 1 cut(s) 135
DriI GACNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 366
Eam1105I GACNNNNNGTC 1 cut(s) 175
EarI CTCTTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 137
Eco72I CACGTG 2 cut(s) 189, 347
EcoRI GAATTC 1 cut(s) 610
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 285, 462
FaqI GGGAC 1 cut(s) 482
FatI CATG 2 cut(s) 281, 458
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 1 cut(s) 338
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 3 cut(s) 219, 567, 630
GlaI GCGC 1 cut(s) 315
GluI GCNGC 1 cut(s) 162
GsaI CCCAGC 1 cut(s) 161
HgaI GACGC 1 cut(s) 264
HhaI GCGC 1 cut(s) 316
Hin1II CATG 2 cut(s) 285, 462
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 4 cut(s) 44, 206, 332, 522
HphI GGTGA 5 cut(s) 46, 207, 245, 251, 257
Hpy166II GTNNAC 4 cut(s) 15, 279, 339, 352
Hpy188I TCNGA 2 cut(s) 496, 616
Hpy188III TCNNGA 2 cut(s) 48, 304
Hpy8I GTNNAC 4 cut(s) 15, 279, 339, 352
HpyAV CCTTC 2 cut(s) 290, 301
HpyCH4III ACNGT 2 cut(s) 132, 152
HpyCH4IV ACGT 3 cut(s) 188, 346, 545
HpyCH4V TGCA 5 cut(s) 186, 258, 378, 408, 530
HpySE526I ACGT 3 cut(s) 188, 346, 545
Hsp92II CATG 2 cut(s) 285, 462
HspAI GCGC 1 cut(s) 314
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 21, 50, 76, 118
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 8 cut(s) 3, 33, 44, 93, 171, 183, 210, 342
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 1 cut(s) 517
MaeI CTAG 3 cut(s) 219, 567, 630
MaeII ACGT 3 cut(s) 188, 346, 545
MaeIII GTNAC 2 cut(s) 152, 454
MalI GATC 4 cut(s) 23, 52, 78, 120
MbiI CCGCTC 1 cut(s) 433
MboI GATC 4 cut(s) 21, 50, 76, 118
MboII GAAGA 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 5 cut(s) 89, 323, 508, 610, 652
MlyI GAGTC 2 cut(s) 326, 531
MnlI CCTC 4 cut(s) 229, 235, 241, 431
MseI TTAA 4 cut(s) 174, 623, 651, 657
MslI CAYNNNNRTG 2 cut(s) 29, 645
MspA1I CMGCKG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 316
NdeII GATC 4 cut(s) 21, 50, 76, 118
NlaIII CATG 2 cut(s) 285, 462
NmuCI GTSAC 2 cut(s) 152, 454
NspI RCATGY 1 cut(s) 285
NspV TTCGAA 1 cut(s) 204
OliI CACNNNNGTG 1 cut(s) 645
PciI ACATGT 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PfeI GAWTC 2 cut(s) 44, 206
PkrI GCNGC 1 cut(s) 163
PleI GAGTC 2 cut(s) 326, 530
PmaCI CACGTG 2 cut(s) 189, 347
PmlI CACGTG 2 cut(s) 189, 347
PpsI GAGTC 2 cut(s) 326, 530
Ppu21I YACGTR 2 cut(s) 189, 347
PscI ACATGT 1 cut(s) 281
PsiI TTATAA 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 196
PspCI CACGTG 2 cut(s) 189, 347
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 196
PspPI GGNCC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 544
RsaNI GTAC 1 cut(s) 543
RseI CAYNNNNRTG 2 cut(s) 29, 645
SaqAI TTAA 4 cut(s) 174, 623, 651, 657
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 4 cut(s) 21, 50, 76, 118
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 2 cut(s) 326, 531
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 1 cut(s) 517
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 204
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 2 cut(s) 29, 645
Sse9I AATT 5 cut(s) 89, 323, 508, 610, 652
SsiI CCGC 3 cut(s) 135, 425, 433
SspMI CTAG 3 cut(s) 219, 567, 630
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 132, 152
TaiI ACGT 3 cut(s) 191, 349, 548
TaqI TCGA 3 cut(s) 204, 520, 525
TasI AATT 5 cut(s) 89, 323, 508, 610, 652
TfiI GAWTC 2 cut(s) 44, 206
Tru1I TTAA 4 cut(s) 174, 623, 651, 657
Tru9I TTAA 4 cut(s) 174, 623, 651, 657
TseFI GTSAC 2 cut(s) 152, 454
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 2 cut(s) 152, 454
TspDTI ATGAA 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 137
XapI RAATTY 3 cut(s) 89, 508, 610
XceI RCATGY 1 cut(s) 285
XmiI GTMKAC 1 cut(s) 338
XspI CTAG 3 cut(s) 219, 567, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.