RchiOBHm_Chr2g0120071

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000099
Physical Location & Seq
Forward (+)
32771309 .. 32774696
3388 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49272

Sequence Viewer

Length: 2385 bp
ATGAATTCTTTTCAATGGTCTATGATCACTACATTGCTCATCTTCCTTCTTTATCTCCCCTCTTGCATTTCCATTGATTCCATTACTCCAAACCAAACCATCAAAGATGGCGACGTTTTAGTCTCAAGCAGGAAGCTCTTTGCGCTAGGGTTCTTCAGCCCTGGAAATTCTGGCAAACGTTACATTGGAGTTTGGTTTAACAAAGTTCCAGAGCAGACCATTGTTTGGGTAGCAAATAGAGACAACCCTATCAATGATTCCTCTGGACTTCTAGTGATCGATGCAGATGGAGGCCTTGTCATATATGGAAAGGACAGAAAACTCCCTCTTTGGTCTGCTAATGTTACTCTCTCTTCTCCAAAAAACTCCATGGCCAAGCTGTTGGATACAGGTAACTTCGTTTTGTTTGAGAATGGGCGGAGCTTGTGGGAAGGGTTTGATTATCCCTCAAATACAATGCTTCCCTTTATGAAAATTGGGCTGAAACGGCGATCCGGGCTTAACCGGTTCCTAACTTCTTGGAAGTCCCAAGATGACCCGGGAACTGGGAATTTCTCTGCTAGGATTGATCCGGATGGGTTTCCGCAGCTGTTTTTGTACAACGGTCAAGCTCCCCGATGGCGGGCCGGATCTTGGATAGGACACAGATGGAGTGGTATCCCTGAAATGACTCGTAATTTCATCTTCAACACTGGTTTTGTGACCAATCAAGATGAGCCATCCGTCATGACTGGCATTAAGGACGACTCAATCTTCTCAAGGATGGTGCTTGATGAATCGGGAATCATCGAACGGTCCATGTGGCAAGATCAGGATCACCGATGGAACAAGTACTGGCATGCCCCGGCAGAACTATGTGACGACTATGGAAAGTGTGGTCCAAATAGCAACTGTGACCCTTACAATCCTGATGAGTTCGAGTGTACATGCCTACCTGGGTTTGAACCTAAGTTGCCCCATGCATGGTACTTGAGAGATGGCTCGGATGGGTGTGTGAGGAAAAAAGGAGCATCCGTGTGTAAAAACGGAGAAGGGTTCGTGAAGGTATCACCAGTAAAGGCACCAGACTCATCTACTACGCATGTAAACTTGACCATGGGTCCGAAAGAATGTGAAGAGGAATGCATAAGGAATTGTTCTTGCATGGCATACTCGGGTGCAGGTGACCCGCAAGAAAGGAATGGATGTGTGACATGGTACGGGGACTTGATGGATACAAGGACTTACACGATTGTCACTCAAGAATTATATGTTCGAGTTGATTCAATTGAATTAGGTAAGGAAAGACAAAATCTATTTCCATTTGGTGTGATCACACCTTCAATCTACTTGGAAGATTCTCCTAGTAGAACAGATGCTGATGATAGCAGATTAAATCCGGATCTTCCTTTCTTTGATCTAAGCACCATATCTGCAGCCACAAAAAATTTCTCTGATTCGAACAGGCTTGGAGAAGGCGGATTCGGCCCCGTTTATAAGGGAGTGCTTTCTACTGGAACAGAAATAGCTGTGAAAAGACTTAGCAAAAATTCTGGCCAGGGAAATGAAGAGTTCAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTGAGAATCTTAGGCTATTGCGTTCAAGATGAAGAGAAGATGCTAATCTATGAATACCTGCCCAACAAAAGCCTGGACTCGTTCATATTCAATGAAATGAAAAGGACATCTCTGGATTGGAAAAGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGGATCTTGTATCTTCATCAAGATTCGAGATTAAGAATTATCCACAGAGACCTAAAGGCCAGCAATATTCTACTGGATTATGATATGAACCCCAAACTTGCAGATTTTGGTATGGCTAGAATATTCAGAGCAAACCAAATTGAAGCAAATACAAATCGCGTGGTTGGAACATATGGTTACATGTCACCGGAGTATGCAATGGAAGGACTATTTTCAGTGAAGTCTGATGTCTACAGTTTCGGTGTTTTGCTGCTAGAAATCGTTACTGGTAGAAAGAACACCGGTTACTACTATGATAATCCTGACTCAAATTTGGTTGGACATGTTTGGGACTTGTGGAAAGAAGGCCGAGCCTTGGAAATCGTCGACCCATCTTTGGGAGAATCCTACCCCATAAGTGAAGTTCTACGGTGCATTCAAATCGCACTCCTGTGCGTGCAAGAGTACGCAAATGACCGGCCAACCATGTCAAAAGTTGTGTCTGTGTTAGGCAACGATGCAGCTCTTCCTTCACCAAGAAAACCTGGATTTCTCTTCAGAAGTAACTATACCAATGGAGACCCCAGTACTAGTACTGCAGAGGCTAATTCCATAAATTATGTTACATGCAGTATAGTTGAAGCTCGCTAA

Protein Analysis

794

Amino Acids

89.35

Weight (kDa)

5.57

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 175 6.4e-31 D-mannose binding lectin
S_locus_glycop PF00954 207 - 317 4.6e-28 S-locus glycoprotein domain
PAN_2 PF08276 340 - 406 2.1e-17 PAN-like domain
Pkinase PF00069 479 - 745 1.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 481 - 747 4.8e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1476
AarI CACCTGC 1 cut(s) 1151
AasI GACNNNNNNGTC 1 cut(s) 119
Acc36I ACCTGC 2 cut(s) 1151, 1665
AccB1I GGYRCC 1 cut(s) 1060
AccB7I CCANNNNNTGG 1 cut(s) 225
AccI GTMKAC 2 cut(s) 1986, 2121
AccII CGCG 1 cut(s) 1914
AccIII TCCGGA 2 cut(s) 571, 1378
AciI CCGC 5 cut(s) 418, 584, 622, 1169, 1458
AclI AACGTT 1 cut(s) 178
AclWI GGATC 6 cut(s) 486, 563, 637, 822, 1389, 1766
AcoI YGGCCR 3 cut(s) 372, 1534, 2213
AcsI RAATTY 6 cut(s) 4, 166, 550, 1426, 1528, 2065
AcuI CTGAAG 2 cut(s) 139, 2275
AfaI GTAC 8 cut(s) 599, 833, 925, 968, 1199, 2201, 2323, 2329
AfiI CCNNNNNNNGG 9 cut(s) 225, 545, 621, 622, 633, 963, 2110, 2131, 2132
AflIII ACRYGT 2 cut(s) 1935, 2077
AgeI ACCGGT 2 cut(s) 504, 2036
AhdI GACNNNNNGTC 1 cut(s) 1098
AhlI ACTAGT 1 cut(s) 2324
AjnI CCWGG 5 cut(s) 160, 934, 1536, 1671, 2278
AjuI GAANNNNNNNTTGG 2 cut(s) 1712, 1744
AleI CACNNNNGTG 1 cut(s) 2185
AluBI AGCT 8 cut(s) 136, 379, 423, 589, 611, 1508, 2258, 2378
AluI AGCT 8 cut(s) 136, 379, 423, 589, 611, 1508, 2258, 2378
Alw26I GTCTC 4 cut(s) 127, 234, 1797, 2307
AlwI GGATC 6 cut(s) 486, 563, 637, 822, 1389, 1766
AlwNI CAGNNNCTG 1 cut(s) 1358
Ama87I CYCGRG 2 cut(s) 538, 1153
Aor13HI TCCGGA 2 cut(s) 571, 1378
AoxI GGCC 8 cut(s) 292, 372, 624, 1465, 1534, 1812, 2101, 2213
ApeKI GCWGC 4 cut(s) 586, 1415, 2005, 2255
ApoI RAATTY 6 cut(s) 4, 166, 550, 1426, 1528, 2065
ArsI GACNNNNNNTTYG 2 cut(s) 2219, 2251
AsiGI ACCGGT 2 cut(s) 504, 2036
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 5 cut(s) 624, 795, 878, 1100, 1466
AsuC2I CCSGG 4 cut(s) 496, 539, 540, 845
AsuHPI GGTGA 5 cut(s) 809, 1041, 1175, 1932, 2259
AsuII TTCGAA 1 cut(s) 1439
AvaI CYCGRG 2 cut(s) 538, 1153
AvaII GGWCC 3 cut(s) 795, 878, 1100
BalI TGGCCA 2 cut(s) 374, 1536
BanI GGYRCC 1 cut(s) 1060
BarI GAAGNNNNNNTAC 2 cut(s) 2287, 2319
BbvI GCAGC 4 cut(s) 598, 1427, 1992, 2267
BceAI ACGGC 1 cut(s) 503
BcgI CGANNNNNNTGC 2 cut(s) 2158, 2192
BciT130I CCWGG 5 cut(s) 162, 936, 1538, 1673, 2280
BciVI GTATCC 3 cut(s) 379, 668, 1207
BclI TGATCA 2 cut(s) 24, 1311
BcnI CCSGG 4 cut(s) 496, 539, 540, 845
BcoDI GTCTC 4 cut(s) 127, 234, 1797, 2307
BcuI ACTAGT 1 cut(s) 2324
BfaI CTAG 8 cut(s) 146, 272, 561, 1344, 1752, 1872, 2009, 2325
BfmI CTRYAG 3 cut(s) 1413, 1987, 2331
BfuAI ACCTGC 2 cut(s) 1151, 1665
BfuI GTATCC 3 cut(s) 379, 668, 1207
BisI GCNGC 4 cut(s) 587, 1416, 2006, 2256
BlsI GCNGC 4 cut(s) 588, 1417, 2007, 2257
BmcAI AGTACT 3 cut(s) 833, 2323, 2329
Bme1390I CCNGG 9 cut(s) 162, 496, 539, 540, 845, 936, 1538, 1673, 2280
Bme18I GGWCC 3 cut(s) 795, 878, 1100
BmeRI GACNNNNNGTC 1 cut(s) 1098
BmeT110I CYCGRG 2 cut(s) 538, 1153
BmgT120I GGNCC 5 cut(s) 624, 795, 878, 1100, 1466
BmiI GGNNCC 5 cut(s) 509, 1062, 1101, 1468, 1595
BmrFI CCNGG 9 cut(s) 162, 496, 539, 540, 845, 936, 1538, 1673, 2280
BmrI ACTGGG 2 cut(s) 555, 2313
BmsI GCATC 5 cut(s) 271, 1019, 1345, 1629, 2242
BmuI ACTGGG 2 cut(s) 555, 2313
Bpu14I TTCGAA 1 cut(s) 1439
BpuEI CTTGAG 4 cut(s) 109, 742, 991, 1224
BpuMI CCSGG 4 cut(s) 496, 539, 540, 845
Bsa29I ATCGAT 1 cut(s) 279
BsaBI GATNNNNATC 2 cut(s) 813, 1643
BsaI GGTCTC 2 cut(s) 1797, 2307
BsaJI CCNNGG 8 cut(s) 160, 369, 538, 843, 935, 1095, 1537, 2109
BsaWI WCCGGW 5 cut(s) 504, 571, 1378, 1942, 2036
BsaXI ACNNNNNCTCC 4 cut(s) 282, 312, 999, 1029
Bsc4I CCNNNNNNNGG 9 cut(s) 225, 545, 621, 622, 633, 963, 2110, 2131, 2132
Bse118I RCCGGY 3 cut(s) 504, 2036, 2211
Bse1I ACTGG 9 cut(s) 550, 697, 736, 839, 1052, 1498, 1833, 2026, 2319
Bse3DI GCAATG 3 cut(s) 32, 1746, 1959
Bse8I GATNNNNATC 2 cut(s) 813, 1643
BseAI TCCGGA 2 cut(s) 571, 1378
BseBI CCWGG 5 cut(s) 162, 936, 1538, 1673, 2280
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 8 cut(s) 160, 369, 538, 843, 935, 1095, 1537, 2109
BseGI GGATG 6 cut(s) 580, 719, 768, 991, 1010, 1190
BseJI GATNNNNATC 2 cut(s) 813, 1643
BseLI CCNNNNNNNGG 9 cut(s) 225, 545, 621, 622, 633, 963, 2110, 2131, 2132
BseMI GCAATG 3 cut(s) 32, 1746, 1959
BseNI ACTGG 9 cut(s) 550, 697, 736, 839, 1052, 1498, 1833, 2026, 2319
BseXI GCAGC 4 cut(s) 598, 1427, 1992, 2267
BsgI GTGCAG 1 cut(s) 1179
Bsh1236I CGCG 1 cut(s) 1914
BshFI GGCC 8 cut(s) 294, 374, 626, 1467, 1536, 1814, 2103, 2215
BshNI GGYRCC 1 cut(s) 1060
BshTI ACCGGT 2 cut(s) 504, 2036
BshVI ATCGAT 1 cut(s) 279
BsiHKCI CYCGRG 2 cut(s) 538, 1153
BslFI GGGAC 3 cut(s) 511, 1217, 2099
BslI CCNNNNNNNGG 9 cut(s) 225, 545, 621, 622, 633, 963, 2110, 2131, 2132
BsmAI GTCTC 4 cut(s) 127, 234, 1797, 2307
BsmFI GGGAC 3 cut(s) 511, 1217, 2099
BsmI GAATGC 2 cut(s) 1127, 2169
BsnI GGCC 8 cut(s) 294, 374, 626, 1467, 1536, 1814, 2103, 2215
Bso31I GGTCTC 2 cut(s) 1797, 2307
BsoBI CYCGRG 2 cut(s) 538, 1153
Bsp119I TTCGAA 1 cut(s) 1439
Bsp13I TCCGGA 2 cut(s) 571, 1378
Bsp1407I TGTACA 2 cut(s) 597, 923
Bsp19I CCATGG 2 cut(s) 369, 1095
BspACI CCGC 5 cut(s) 418, 584, 622, 1169, 1458
BspANI GGCC 8 cut(s) 294, 374, 626, 1467, 1536, 1814, 2103, 2215
BspDI ATCGAT 1 cut(s) 279
BspEI TCCGGA 2 cut(s) 571, 1378
BspFNI CGCG 1 cut(s) 1914
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 5 cut(s) 509, 1062, 1101, 1468, 1595
BspMAI CTGCAG 2 cut(s) 1417, 2335
BspMI ACCTGC 2 cut(s) 1151, 1665
BspPI GGATC 6 cut(s) 486, 563, 637, 822, 1389, 1766
BspQI GCTCTTC 1 cut(s) 2265
BspT104I TTCGAA 1 cut(s) 1439
BspT107I GGYRCC 1 cut(s) 1060
BspTNI GGTCTC 2 cut(s) 1797, 2307
BsrDI GCAATG 3 cut(s) 32, 1746, 1959
BsrFI RCCGGY 3 cut(s) 504, 2036, 2211
BsrGI TGTACA 2 cut(s) 597, 923
BsrI ACTGG 9 cut(s) 550, 697, 736, 839, 1052, 1498, 1833, 2026, 2319
BssAI RCCGGY 3 cut(s) 504, 2036, 2211
BssECI CCNNGG 8 cut(s) 160, 369, 538, 843, 935, 1095, 1537, 2109
BssT1I CCWWGG 3 cut(s) 369, 1095, 2109
Bst2UI CCWGG 5 cut(s) 162, 936, 1538, 1673, 2280
Bst4CI ACNGT 5 cut(s) 605, 795, 893, 1991, 2166
Bst6I CTCTTC 6 cut(s) 358, 1110, 1542, 1626, 2265, 2294
BstAUI TGTACA 2 cut(s) 597, 923
BstBI TTCGAA 1 cut(s) 1439
BstC8I GCNNGC 5 cut(s) 624, 840, 1816, 2192, 2380
BstDEI CTNAG 4 cut(s) 948, 1400, 1520, 1609
BstDSI CCRYGG 2 cut(s) 369, 1095
BstEII GGTNACC 1 cut(s) 1163
BstF5I GGATG 6 cut(s) 580, 719, 768, 991, 1010, 1190
BstFNI CGCG 1 cut(s) 1914
BstHHI GCGC 1 cut(s) 145
BstMAI GTCTC 4 cut(s) 127, 234, 1797, 2307
BstMWI GCNNNNNNNGC 4 cut(s) 142, 487, 496, 1464
BstNI CCWGG 5 cut(s) 162, 936, 1538, 1673, 2280
BstNSI RCATGY 6 cut(s) 842, 930, 1085, 1939, 2081, 2364
BstPI GGTNACC 1 cut(s) 1163
BstSCI CCNGG 9 cut(s) 160, 494, 537, 538, 843, 934, 1536, 1671, 2278
BstSFI CTRYAG 3 cut(s) 1413, 1987, 2331
BstUI CGCG 1 cut(s) 1914
BstV1I GCAGC 4 cut(s) 598, 1427, 1992, 2267
BstX2I RGATCY 3 cut(s) 629, 1381, 1758
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 3 cut(s) 629, 1381, 1758
Bsu15I ATCGAT 1 cut(s) 279
BsuI GTATCC 3 cut(s) 379, 668, 1207
BsuRI GGCC 8 cut(s) 294, 374, 626, 1467, 1536, 1814, 2103, 2215
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 2 cut(s) 369, 1095
BtsCI GGATG 6 cut(s) 580, 719, 768, 991, 1010, 1190
BtsIMutI CAGTG 2 cut(s) 690, 1977
BveI ACCTGC 2 cut(s) 1151, 1665
Cac8I GCNNGC 5 cut(s) 624, 840, 1816, 2192, 2380
CaiI CAGNNNCTG 1 cut(s) 1358
CciI TCATGA 1 cut(s) 726
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 3 cut(s) 504, 2036, 2211
Cfr13I GGNCC 5 cut(s) 624, 795, 878, 1100, 1466
Cfr9I CCCGGG 1 cut(s) 538
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 1 cut(s) 1734
Csp6I GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2200, 2322, 2328
CspAI ACCGGT 2 cut(s) 504, 2036
CspCI CAANNNNNGTGG 2 cut(s) 1896, 1931
CviQI GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2200, 2322, 2328
DdeI CTNAG 4 cut(s) 948, 1400, 1520, 1609
DrdI GACNNNNNNGTC 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 1098
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 3 cut(s) 372, 1534, 2213
Eam1104I CTCTTC 6 cut(s) 358, 1110, 1542, 1626, 2265, 2294
Eam1105I GACNNNNNGTC 1 cut(s) 1098
EarI CTCTTC 6 cut(s) 358, 1110, 1542, 1626, 2265, 2294
EciI GGCGGA 2 cut(s) 433, 1473
Eco130I CCWWGG 3 cut(s) 369, 1095, 2109
Eco147I AGGCCT 1 cut(s) 294
Eco31I GGTCTC 2 cut(s) 1797, 2307
Eco47I GGWCC 3 cut(s) 795, 878, 1100
Eco57I CTGAAG 2 cut(s) 139, 2275
Eco88I CYCGRG 2 cut(s) 538, 1153
Eco91I GGTNACC 1 cut(s) 1163
EcoO65I GGTNACC 1 cut(s) 1163
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 5 cut(s) 160, 934, 1536, 1671, 2278
EcoT14I CCWWGG 3 cut(s) 369, 1095, 2109
EcoT22I ATGCAT 2 cut(s) 964, 1127
ErhI CCWWGG 3 cut(s) 369, 1095, 2109
FaqI GGGAC 3 cut(s) 511, 1217, 2099
FauI CCCGC 2 cut(s) 615, 1176
FauNDI CATATG 1 cut(s) 1927
FbaI TGATCA 2 cut(s) 24, 1311
FblI GTMKAC 2 cut(s) 1986, 2121
Fnu4HI GCNGC 4 cut(s) 587, 1416, 2006, 2256
FokI GGATG 6 cut(s) 587, 706, 775, 997, 998, 1197
Fsp4HI GCNGC 4 cut(s) 587, 1416, 2006, 2256
FspBI CTAG 8 cut(s) 146, 272, 561, 1344, 1752, 1872, 2009, 2325
GlaI GCGC 1 cut(s) 144
GluI GCNGC 4 cut(s) 587, 1416, 2006, 2256
HaeIII GGCC 8 cut(s) 294, 374, 626, 1467, 1536, 1814, 2103, 2215
HgaI GACGC 1 cut(s) 1734
HhaI GCGC 1 cut(s) 145
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HincII GTYRAC 1 cut(s) 2122
HindII GTYRAC 1 cut(s) 2122
HphI GGTGA 5 cut(s) 809, 1041, 1175, 1932, 2259
Hpy166II GTNNAC 4 cut(s) 924, 1087, 1987, 2122
Hpy188I TCNGA 6 cut(s) 985, 1104, 1435, 1883, 1981, 2294
Hpy8I GTNNAC 4 cut(s) 924, 1087, 1987, 2122
Hpy99I CGWCG 2 cut(s) 116, 2123
HpyAV CCTTC 9 cut(s) 56, 425, 1025, 1036, 1329, 1448, 1952, 2093, 2274
HpyCH4III ACNGT 5 cut(s) 605, 795, 893, 1991, 2166
HpyCH4IV ACGT 2 cut(s) 114, 178
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 487, 496, 1464
HpyF3I CTNAG 4 cut(s) 948, 1400, 1520, 1609
HpySE526I ACGT 2 cut(s) 114, 178
HspAI GCGC 1 cut(s) 143
Kpn2I TCCGGA 2 cut(s) 571, 1378
Ksp22I TGATCA 2 cut(s) 24, 1311
LguI GCTCTTC 1 cut(s) 2265
LmnI GCTCC 3 cut(s) 420, 616, 1007
Lsp1109I GCAGC 4 cut(s) 598, 1427, 1992, 2267
LweI GCATC 5 cut(s) 271, 1019, 1345, 1629, 2242
MaeI CTAG 8 cut(s) 146, 272, 561, 1344, 1752, 1872, 2009, 2325
MaeII ACGT 2 cut(s) 114, 178
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 3 cut(s) 629, 1381, 1758
MlsI TGGCCA 2 cut(s) 374, 1536
MluNI TGGCCA 2 cut(s) 374, 1536
MlyI GAGTC 5 cut(s) 664, 740, 1061, 1670, 2054
MmeI TCCRAC 3 cut(s) 363, 1900, 2053
MnlI CCTC 9 cut(s) 70, 271, 284, 336, 457, 990, 1111, 1748, 2329
Mox20I TGGCCA 2 cut(s) 374, 1536
Mph1103I ATGCAT 2 cut(s) 964, 1127
MroI TCCGGA 2 cut(s) 571, 1378
MscI TGGCCA 2 cut(s) 374, 1536
MseI TTAA 5 cut(s) 198, 501, 738, 1373, 1787
MslI CAYNNNNRTG 2 cut(s) 1015, 2185
Msp20I TGGCCA 2 cut(s) 374, 1536
MspA1I CMGCKG 1 cut(s) 589
MspR9I CCNGG 9 cut(s) 162, 496, 539, 540, 845, 936, 1538, 1673, 2280
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 2 cut(s) 1127, 2169
MvaI CCWGG 5 cut(s) 162, 936, 1538, 1673, 2280
MvnI CGCG 1 cut(s) 1914
MwoI GCNNNNNNNGC 4 cut(s) 142, 487, 496, 1464
NciI CCSGG 4 cut(s) 496, 539, 540, 845
NcoI CCATGG 2 cut(s) 369, 1095
NdeI CATATG 1 cut(s) 1927
NlaIV GGNNCC 5 cut(s) 509, 1062, 1101, 1468, 1595
NmeAIII GCCGAG 1 cut(s) 2129
NmuCI GTSAC 7 cut(s) 700, 857, 893, 1163, 1189, 1234, 1938
NsiI ATGCAT 2 cut(s) 964, 1127
NspI RCATGY 6 cut(s) 842, 930, 1085, 1939, 2081, 2364
NspV TTCGAA 1 cut(s) 1439
OliI CACNNNNGTG 1 cut(s) 2185
PaeI GCATGC 1 cut(s) 842
PagI TCATGA 1 cut(s) 726
PaqCI CACCTGC 1 cut(s) 1151
PceI AGGCCT 1 cut(s) 294
PciI ACATGT 2 cut(s) 1935, 2077
PciSI GCTCTTC 1 cut(s) 2265
PctI GAATGC 2 cut(s) 1127, 2169
PflMI CCANNNNNTGG 1 cut(s) 225
PinAI ACCGGT 2 cut(s) 504, 2036
PkrI GCNGC 4 cut(s) 588, 1417, 2007, 2257
PleI GAGTC 5 cut(s) 664, 740, 1061, 1670, 2054
PpsI GAGTC 5 cut(s) 664, 740, 1061, 1670, 2054
PscI ACATGT 2 cut(s) 1935, 2077
PsiI TTATAA 1 cut(s) 1476
Psp1406I AACGTT 1 cut(s) 178
Psp6I CCWGG 5 cut(s) 160, 934, 1536, 1671, 2278
PspEI GGTNACC 1 cut(s) 1163
PspGI CCWGG 5 cut(s) 160, 934, 1536, 1671, 2278
PspN4I GGNNCC 5 cut(s) 509, 1062, 1101, 1468, 1595
PspPI GGNCC 5 cut(s) 624, 795, 878, 1100, 1466
PsrI GAACNNNNNNTAC 2 cut(s) 2024, 2056
PstI CTGCAG 2 cut(s) 1417, 2335
PstNI CAGNNNCTG 1 cut(s) 1358
PsuI RGATCY 3 cut(s) 629, 1381, 1758
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 8 cut(s) 599, 833, 925, 968, 1199, 2201, 2323, 2329
RsaNI GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2200, 2322, 2328
RseI CAYNNNNRTG 2 cut(s) 1015, 2185
SalI GTCGAC 1 cut(s) 2120
SapI GCTCTTC 1 cut(s) 2265
SaqAI TTAA 5 cut(s) 198, 501, 738, 1373, 1787
SatI GCNGC 4 cut(s) 587, 1416, 2006, 2256
Sau96I GGNCC 5 cut(s) 624, 795, 878, 1100, 1466
ScaI AGTACT 3 cut(s) 833, 2323, 2329
SchI GAGTC 5 cut(s) 664, 740, 1061, 1670, 2054
ScrFI CCNGG 9 cut(s) 162, 496, 539, 540, 845, 936, 1538, 1673, 2280
SfaNI GCATC 5 cut(s) 271, 1019, 1345, 1629, 2242
SfcI CTRYAG 3 cut(s) 1413, 1987, 2331
SfuI TTCGAA 1 cut(s) 1439
SinI GGWCC 3 cut(s) 795, 878, 1100
SmaI CCCGGG 1 cut(s) 540
SmiMI CAYNNNNRTG 2 cut(s) 1015, 2185
SmlI CTYRAG 4 cut(s) 124, 757, 970, 1239
SmoI CTYRAG 4 cut(s) 124, 757, 970, 1239
SpeI ACTAGT 1 cut(s) 2324
SphI GCATGC 1 cut(s) 842
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 5 cut(s) 418, 584, 622, 1169, 1458
SspI AATATT 2 cut(s) 1822, 1878
SspMI CTAG 8 cut(s) 146, 272, 561, 1344, 1752, 1872, 2009, 2325
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 9 cut(s) 160, 494, 537, 538, 843, 934, 1536, 1671, 2278
StyI CCWWGG 3 cut(s) 369, 1095, 2109
TaaI ACNGT 5 cut(s) 605, 795, 893, 1991, 2166
TaiI ACGT 2 cut(s) 117, 181
TaqI TCGA 7 cut(s) 279, 789, 918, 1255, 1439, 1781, 2121
TatI WGTACW 5 cut(s) 597, 831, 923, 2321, 2327
Tru1I TTAA 5 cut(s) 198, 501, 738, 1373, 1787
Tru9I TTAA 5 cut(s) 198, 501, 738, 1373, 1787
TscAI CASTG 2 cut(s) 697, 1977
TseFI GTSAC 7 cut(s) 700, 857, 893, 1163, 1189, 1234, 1938
TseI GCWGC 4 cut(s) 586, 1415, 2005, 2255
Tsp45I GTSAC 7 cut(s) 700, 857, 893, 1163, 1189, 1234, 1938
TspGWI ACGGA 3 cut(s) 712, 1003, 1041
TspMI CCCGGG 1 cut(s) 538
TspRI CASTG 2 cut(s) 697, 1977
Van91I CCANNNNNTGG 1 cut(s) 225
VpaK11BI GGWCC 3 cut(s) 795, 878, 1100
XapI RAATTY 6 cut(s) 4, 166, 550, 1426, 1528, 2065
XceI RCATGY 6 cut(s) 842, 930, 1085, 1939, 2081, 2364
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XmaI CCCGGG 1 cut(s) 538
XmiI GTMKAC 2 cut(s) 1986, 2121
XspI CTAG 8 cut(s) 146, 272, 561, 1344, 1752, 1872, 2009, 2325
ZrmI AGTACT 3 cut(s) 833, 2323, 2329
Zsp2I ATGCAT 2 cut(s) 964, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.