RchiOBHm_Chr5g0005041

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000099
Physical Location & Seq
Reverse (-)
3302396 .. 3305741
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28627

Sequence Viewer

Length: 2400 bp
ATGCATTTTGCAAAAGACTTTCTGAATACATTGCTTCAGCTTCTTCTTCTTCTTCAGCTTTGCCATTCTTTGGACACAACTATCACTTCAAGCAAATCCATCAAAGATGGTGACTTCCTTGTCTCTGAAAACCAGACTTTTGTGCTTGGATTCTTTAGTCCTGGTACATCAAGCAACCGCTATGTTGGTATTTGGTACGAGTTCTCAGAAGACATGGTTGTGTGGGTAGCTAACAGAGACAATCCTGTCAATGATACCTCAGGTATCCTCACAATTAGTGCTGATGGAAATCTTGCACTACTTCATAACAGCAGTCGAGGCCCTCCTCTGTGGTCAACAAATGTTTCTGTCTCGCCAGTAAGTAACAATAGTACTATGGTGCAGCTCCTGGATTCAGGAAATCTTGTCTTGGTTAATCAGCTGGTCAACAACCAGAGTGGTGTCTTATGGCAGAGCTTTGATCATCCTACACATGTTCTTCTTGCAAACATGAAAATTGGGTTGGACCATGGTAAGAACATATTCCTCACATCCTGGAACTCAAAAAATGACCCAGGAACTGGGGTTTGCTCATTAAAGACAGAACCAAACGGGTCCCCACAAATAATCTTGTACAAGAGTAGGACAAAGTGGTGGCGGTCAGGTCAATGGAATGGGCTTCACTGGAATGGATTACCAAACATAAACAGACAAAGCAACAATCATGCATTTGACATAGCTTTTGTGAACAACAAAGATGAGGTTACTACAAAATGGCTTGTTCTTGACCCATCAGTAATCTCGGTAATAGCAATTGACGGATCTGGATCAATCCAACAACTAGCATGGCAGGGACAGCGGCAAGGGTGGGTCACAATCTGGTCAGCACCAACAGATGTGTGTGACAAGTATGGTAGATGTGGTCCATTTGGTATTTGTAATACATACACTGCTAACTGGGAAAAATGTGCATGCTTTCCTGGATACGAACCCAACTCCCCAGAGGATTGGAAATTAAGAGAAGGGTCAGGTGGGTGCAAGAGGCAGAAGGGAGCTCCATCCATGTGTAAGAATGGAGAGGAATTTGTAAAGATACCAAACGTGAAGGTTCCAGATCCTTCCACAATCAAATTGGACATCAATTTGACTATGGCAGCATGTGAGCAGAAGTGTTTGAGTAACTGCTCATGCTTGGCATATGCCAGTGCAGATGTGAGGAACGGAGGCAGCGGATGCATGACATGGTATGGAGATTTAATGGACACTACGCAATTTACTCAGGGACAAGGACAAGATCTGTACATACGAGCTGATGCAATAGTCACAGTACAGAAAGGAGTCAAAGAGGTTGCTTGCCAAAAAGGGGATGCTGTTGATTTTGACCCTTATAGGGACACGACGCAAGATGGAAGTAAAACTCACAAAGACCTGGAATATTTTGACCTAAAGAGCATACTAGTTGCCACTGACAATTTCTCTGCTTCTAACAAGCTAGGCGAAGGAGGATTTGGCTCAGTATATAAGGGTATACTAGCTGATGGGGAGGAGATTGCTGTCAAAAGATTATCAAAGCACTCTGGACAAGGTTTAGAACAATTCAAAACTGAGGTTAAGCTTATATCAAAACTCCAGCACAGAAACCTGGTGAGGCTTCTTGGTTGTTGCATCAATTCAGAAGAGAAGATGTTGATATATGAATACTTACCAAACAAAAGCTTGGACTTTTTCCTATTTGATAGAAGTAGAAGCTCGTTGTTAGACTGGAAAGAACGTTTTGACATTATTATTGGAATTGCTCGAGGGGTCCTATATCTCCATCAAGACTCAAGATTAAAAATTATCCACAGGGACATAAAAGCTAGCAACATCTTATTGGATTCCACAATGAACCCAAAGATCTCAGATTTTGGACTAGCTAAAATCATTGGGGAAGACCAAATCCAAACAAATACAAACAGAGTGATTGGAACGTATGGATATATGTCACCTGAATATGCAATGGAAGGGCGATATTCAACAAAATCTGATGTTTTTAGCTATGGTGTCTTACTGCTAGAGATCATCAGTGGCAAGAGGAACAGTGACTACGATATTGAACGCCGCACCCCCAATTTGATAGGACAAATTTGGGAAATGTGGAGAGAGAGACAAACATCAGGAATAGTTGATCCTTCTTTGAGTCCGTCATATCCTGTTCATGAAGTTTCCAGATGCATCCAGATTGGGCTACTTTGCGTGCAAGAATATGCAACAGATCGGCCAACAATGTCAGAAGTTGTTTGCATGTTGGGGAAAGACACACCTCTTACTTCTCCTAAGAAACCTGCATTCATATTACAGTCCAGCAATCCAAACTCAGAAGCACCAAGAAGAGTACCTTCTATAAACAATGTAACAATAACCATGCTTGAAGCCCGCTAA

Protein Analysis

799

Amino Acids

89.07

Weight (kDa)

6.31

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 179 2.8e-30 D-mannose binding lectin
S_locus_glycop PF00954 212 - 325 2.1e-26 S-locus glycoprotein domain
PAN_2 PF08276 349 - 415 5.7e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 487 - 755 2.6e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 487 - 753 8.6e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 119, 245
Acc36I ACCTGC 1 cut(s) 2311
AccB7I CCANNNNNTGG 2 cut(s) 70, 560
AccI GTMKAC 1 cut(s) 1507
AciI CCGC 6 cut(s) 178, 637, 838, 1209, 2080, 2395
AclI AACGTT 1 cut(s) 1750
AclWI GGATC 4 cut(s) 808, 814, 1088, 2141
AcoI YGGCCR 1 cut(s) 2237
AcsI RAATTY 2 cut(s) 1061, 2103
AcuI CTGAAG 2 cut(s) 20, 38
AfaI GTAC 7 cut(s) 166, 197, 373, 614, 1280, 1308, 2355
AfiI CCNNNNNNNGG 4 cut(s) 70, 560, 1342, 1369
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 5 cut(s) 90, 1579, 1995, 2075, 2390
AhlI ACTAGT 1 cut(s) 1435
AjnI CCWGG 7 cut(s) 160, 387, 533, 553, 958, 1407, 1620
AjuI GAANNNNNNNTTGG 4 cut(s) 485, 517, 1470, 1502
Alw21I GWGCWC 1 cut(s) 1036
Alw26I GTCTC 4 cut(s) 127, 231, 355, 2119
AlwI GGATC 4 cut(s) 808, 814, 1088, 2141
AlwNI CAGNNNCTG 2 cut(s) 388, 560
Ama87I CYCGRG 1 cut(s) 1776
AoxI GGCC 2 cut(s) 319, 2237
ApeKI GCWGC 3 cut(s) 382, 1133, 1206
ApoI RAATTY 2 cut(s) 1061, 2103
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 5 cut(s) 320, 505, 594, 902, 1783
AsuHPI GGTGA 3 cut(s) 122, 1636, 1956
AsuNHI GCTAGC 1 cut(s) 1838
AvaI CYCGRG 1 cut(s) 1776
AvaII GGWCC 4 cut(s) 505, 594, 902, 1783
AxyI CCTNAGG 1 cut(s) 259
BanII GRGCYC 1 cut(s) 1036
BbsI GAAGAC 2 cut(s) 216, 1917
Bbv12I GWGCWC 1 cut(s) 1036
BbvI GCAGC 3 cut(s) 394, 1145, 1218
BccI CCATC 8 cut(s) 101, 107, 278, 778, 1045, 1379, 1511, 1803
BciT130I CCWGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
BciVI GTATCC 2 cut(s) 275, 956
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 4 cut(s) 127, 231, 355, 2119
BcuI ACTAGT 1 cut(s) 1435
BfaI CTAG 7 cut(s) 821, 1436, 1472, 1511, 1839, 1892, 2033
BfuAI ACCTGC 1 cut(s) 2311
BfuI GTATCC 2 cut(s) 275, 956
BglII AGATCT 2 cut(s) 1273, 1875
BisI GCNGC 5 cut(s) 383, 839, 1134, 1207, 2080
BlsI GCNGC 5 cut(s) 384, 840, 1135, 1208, 2081
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
Bme18I GGWCC 4 cut(s) 505, 594, 902, 1783
BmeT110I CYCGRG 1 cut(s) 1776
BmgT120I GGNCC 5 cut(s) 320, 505, 594, 902, 1783
BmiI GGNNCC 4 cut(s) 595, 596, 1089, 1784
BmrFI CCNGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
BmrI ACTGGG 2 cut(s) 570, 946
BmsI GCATC 6 cut(s) 1202, 1282, 1336, 1653, 2180, 2202
BmtI GCTAGC 1 cut(s) 1842
BmuI ACTGGG 2 cut(s) 570, 946
BpiI GAAGAC 2 cut(s) 216, 1917
BpmI CTGGAG 1 cut(s) 1592
BpuEI CTTGAG 1 cut(s) 1789
BsaBI GATNNNNATC 2 cut(s) 288, 805
BsaJI CCNNGG 2 cut(s) 508, 553
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 560, 1342, 1369
Bse1I ACTGG 6 cut(s) 356, 565, 668, 941, 1182, 1745
Bse21I CCTNAGG 1 cut(s) 259
Bse3DI GCAATG 2 cut(s) 29, 1983
Bse8I GATNNNNATC 2 cut(s) 288, 805
BseBI CCWGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
BseDI CCNNGG 2 cut(s) 508, 553
BseGI GGATG 6 cut(s) 463, 530, 1037, 1217, 1351, 2193
BseJI GATNNNNATC 2 cut(s) 288, 805
BseLI CCNNNNNNNGG 4 cut(s) 70, 560, 1342, 1369
BseMI GCAATG 2 cut(s) 29, 1983
BseMII CTCAG 7 cut(s) 219, 273, 1271, 1506, 1575, 1893, 2349
BseNI ACTGG 6 cut(s) 356, 565, 668, 941, 1182, 1745
BseRI GAGGAG 2 cut(s) 315, 1538
BseXI GCAGC 3 cut(s) 394, 1145, 1218
BsgI GTGCAG 2 cut(s) 401, 1206
BshFI GGCC 2 cut(s) 321, 2239
BsiHKAI GWGCWC 1 cut(s) 1036
BsiHKCI CYCGRG 1 cut(s) 1776
BslFI GGGAC 5 cut(s) 580, 846, 1275, 1385, 1841
BslI CCNNNNNNNGG 4 cut(s) 70, 560, 1342, 1369
BsmAI GTCTC 4 cut(s) 127, 231, 355, 2119
BsmFI GGGAC 5 cut(s) 580, 846, 1275, 1385, 1841
BsmI GAATGC 1 cut(s) 2306
BsnI GGCC 2 cut(s) 321, 2239
BsoBI CYCGRG 1 cut(s) 1776
Bsp1286I GDGCHC 1 cut(s) 1036
Bsp1407I TGTACA 2 cut(s) 612, 1278
Bsp143I GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 6 cut(s) 178, 637, 838, 1209, 2080, 2395
BspANI GGCC 2 cut(s) 321, 2239
BspCNI CTCAG 7 cut(s) 218, 272, 1270, 1505, 1576, 1892, 2348
BspHI TCATGA 1 cut(s) 2176
BspLI GGNNCC 4 cut(s) 595, 596, 1089, 1784
BspMI ACCTGC 1 cut(s) 2311
BspOI GCTAGC 1 cut(s) 1842
BspPI GGATC 4 cut(s) 808, 814, 1088, 2141
BsrDI GCAATG 2 cut(s) 29, 1983
BsrGI TGTACA 2 cut(s) 612, 1278
BsrI ACTGG 6 cut(s) 356, 565, 668, 941, 1182, 1745
BssECI CCNNGG 2 cut(s) 508, 553
BssMI GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
BssNAI GTATAC 1 cut(s) 1508
BssT1I CCWWGG 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 1508
Bst2UI CCWGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
Bst4CI ACNGT 3 cut(s) 1306, 2060, 2319
Bst6I CTCTTC 2 cut(s) 1650, 2344
BstAPI GCANNNNNTGC 1 cut(s) 1212
BstAUI TGTACA 2 cut(s) 612, 1278
BstC8I GCNNGC 5 cut(s) 952, 1333, 1840, 2216, 2395
BstDEI CTNAG 8 cut(s) 205, 259, 1257, 1492, 1584, 1879, 2295, 2335
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 6 cut(s) 463, 530, 1037, 1217, 1351, 2193
BstKTI GATC 9 cut(s) 463, 803, 809, 1096, 1276, 1878, 2040, 2149, 2236
BstMAI GTCTC 4 cut(s) 127, 231, 355, 2119
BstMBI GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
BstMWI GCNNNNNNNGC 3 cut(s) 318, 835, 1212
BstNI CCWGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
BstNSI RCATGY 4 cut(s) 476, 954, 1140, 2266
BstSCI CCNGG 7 cut(s) 160, 387, 533, 553, 958, 1407, 1620
BstV1I GCAGC 3 cut(s) 394, 1145, 1218
BstV2I GAAGAC 2 cut(s) 216, 1917
BstX2I RGATCY 4 cut(s) 800, 1093, 1273, 1875
BstXI CCANNNNNNTGG 1 cut(s) 987
BstYI RGATCY 4 cut(s) 800, 1093, 1273, 1875
BstZ17I GTATAC 1 cut(s) 1508
Bsu36I CCTNAGG 1 cut(s) 259
BsuI GTATCC 2 cut(s) 275, 956
BsuRI GGCC 2 cut(s) 321, 2239
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 6 cut(s) 463, 530, 1037, 1217, 1351, 2193
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 6 cut(s) 661, 927, 1189, 1443, 2050, 2065
BveI ACCTGC 1 cut(s) 2311
Cac8I GCNNGC 5 cut(s) 952, 1333, 1840, 2216, 2395
CaiI CAGNNNCTG 2 cut(s) 388, 560
CciI TCATGA 1 cut(s) 2176
Cfr13I GGNCC 5 cut(s) 320, 505, 594, 902, 1783
CseI GACGC 1 cut(s) 1387
CsiI ACCWGGT 1 cut(s) 1620
Csp6I GTAC 7 cut(s) 165, 196, 372, 613, 1279, 1307, 2354
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviQI GTAC 7 cut(s) 165, 196, 372, 613, 1279, 1307, 2354
DdeI CTNAG 8 cut(s) 205, 259, 1257, 1492, 1584, 1879, 2295, 2335
DpnI GATC 9 cut(s) 462, 802, 808, 1095, 1275, 1877, 2039, 2148, 2235
DpnII GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
DrdI GACNNNNNNGTC 2 cut(s) 119, 245
DseDI GACNNNNNNGTC 2 cut(s) 119, 245
EaeI YGGCCR 1 cut(s) 2237
Eam1104I CTCTTC 2 cut(s) 1650, 2344
EarI CTCTTC 2 cut(s) 1650, 2344
Ecl136II GAGCTC 1 cut(s) 1034
Eco130I CCWWGG 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 1036
Eco47I GGWCC 4 cut(s) 505, 594, 902, 1783
Eco53kI GAGCTC 1 cut(s) 1034
Eco57I CTGAAG 2 cut(s) 20, 38
Eco81I CCTNAGG 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 1776
EcoICRI GAGCTC 1 cut(s) 1034
EcoO109I RGGNCCY 3 cut(s) 320, 594, 1783
EcoRII CCWGG 7 cut(s) 160, 387, 533, 553, 958, 1407, 1620
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 4 cut(s) 6, 709, 1217, 2195
EcoT38I GRGCYC 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 508
FalI AAGNNNNNCTT 2 cut(s) 2341, 2373
FaqI GGGAC 5 cut(s) 580, 846, 1275, 1385, 1841
FauNDI CATATG 1 cut(s) 1177
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 1507
Fnu4HI GCNGC 5 cut(s) 383, 839, 1134, 1207, 2080
FokI GGATG 6 cut(s) 450, 517, 1024, 1224, 1358, 2180
FriOI GRGCYC 1 cut(s) 1036
Fsp4HI GCNGC 5 cut(s) 383, 839, 1134, 1207, 2080
FspBI CTAG 7 cut(s) 821, 1436, 1472, 1511, 1839, 1892, 2033
GluI GCNGC 5 cut(s) 383, 839, 1134, 1207, 2080
GsuI CTGGAG 1 cut(s) 1592
HaeIII GGCC 2 cut(s) 321, 2239
HgaI GACGC 1 cut(s) 1387
HincII GTYRAC 2 cut(s) 336, 427
HindII GTYRAC 2 cut(s) 336, 427
HindIII AAGCTT 2 cut(s) 1592, 1693
HinfI GANTC 6 cut(s) 150, 392, 1317, 1802, 1856, 2158
HphI GGTGA 3 cut(s) 122, 1636, 1956
Hpy166II GTNNAC 4 cut(s) 336, 427, 727, 1508
Hpy188I TCNGA 8 cut(s) 24, 127, 208, 1654, 1882, 2005, 2251, 2338
Hpy8I GTNNAC 4 cut(s) 336, 427, 727, 1508
Hpy99I CGWCG 1 cut(s) 1381
HpyAV CCTTC 8 cut(s) 995, 1021, 1078, 1107, 1472, 1976, 2160, 2367
HpyCH4III ACNGT 3 cut(s) 1306, 2060, 2319
HpyCH4IV ACGT 3 cut(s) 1080, 1750, 1949
HpyF10VI GCNNNNNNNGC 3 cut(s) 318, 835, 1212
HpyF3I CTNAG 8 cut(s) 205, 259, 1257, 1492, 1584, 1879, 2295, 2335
HpySE526I ACGT 3 cut(s) 1080, 1750, 1949
KflI GGGWCCC 1 cut(s) 594
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
LmnI GCTCC 3 cut(s) 390, 1031, 1039
Lsp1109I GCAGC 3 cut(s) 394, 1145, 1218
LweI GCATC 6 cut(s) 1202, 1282, 1336, 1653, 2180, 2202
MabI ACCWGGT 1 cut(s) 1620
MaeI CTAG 7 cut(s) 821, 1436, 1472, 1511, 1839, 1892, 2033
MaeII ACGT 3 cut(s) 1080, 1750, 1949
MalI GATC 9 cut(s) 462, 802, 808, 1095, 1275, 1877, 2039, 2148, 2235
MboI GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 4 cut(s) 800, 1093, 1273, 1875
MhlI GDGCHC 1 cut(s) 1036
MlyI GAGTC 3 cut(s) 1326, 1796, 2167
MmeI TCCRAC 2 cut(s) 483, 838
Mph1103I ATGCAT 4 cut(s) 6, 709, 1217, 2195
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 6 cut(s) 414, 575, 995, 1235, 1590, 1811
MslI CAYNNNNRTG 3 cut(s) 218, 666, 1042
MspA1I CMGCKG 3 cut(s) 421, 838, 1209
MspR9I CCNGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
MunI CAATTG 1 cut(s) 792
Mva1269I GAATGC 1 cut(s) 2306
MvaI CCWGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
MwoI GCNNNNNNNGC 3 cut(s) 318, 835, 1212
NcoI CCATGG 1 cut(s) 508
NdeI CATATG 1 cut(s) 1177
NdeII GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
NheI GCTAGC 1 cut(s) 1838
NlaIV GGNNCC 4 cut(s) 595, 596, 1089, 1784
NmuCI GTSAC 6 cut(s) 110, 850, 881, 1300, 1962, 2060
NsiI ATGCAT 4 cut(s) 6, 709, 1217, 2195
NspI RCATGY 4 cut(s) 476, 954, 1140, 2266
PaeI GCATGC 1 cut(s) 954
PaeR7I CTCGAG 1 cut(s) 1776
PagI TCATGA 1 cut(s) 2176
PciI ACATGT 1 cut(s) 472
PctI GAATGC 1 cut(s) 2306
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 150, 392, 1856
PflMI CCANNNNNTGG 2 cut(s) 70, 560
PfoI TCCNGGA 3 cut(s) 387, 533, 958
PkrI GCNGC 5 cut(s) 384, 840, 1135, 1208, 2081
PleI GAGTC 3 cut(s) 1325, 1796, 2166
PpsI GAGTC 3 cut(s) 1325, 1796, 2166
PpuMI RGGWCCY 2 cut(s) 594, 1783
PscI ACATGT 1 cut(s) 472
Psp124BI GAGCTC 1 cut(s) 1036
Psp1406I AACGTT 1 cut(s) 1750
Psp5II RGGWCCY 2 cut(s) 594, 1783
Psp6I CCWGG 7 cut(s) 160, 387, 533, 553, 958, 1407, 1620
PspGI CCWGG 7 cut(s) 160, 387, 533, 553, 958, 1407, 1620
PspN4I GGNNCC 4 cut(s) 595, 596, 1089, 1784
PspPI GGNCC 5 cut(s) 320, 505, 594, 902, 1783
PspPPI RGGWCCY 2 cut(s) 594, 1783
PspXI VCTCGAGB 1 cut(s) 1776
PsrI GAACNNNNNNTAC 2 cut(s) 2048, 2080
PstNI CAGNNNCTG 2 cut(s) 388, 560
PsuI RGATCY 4 cut(s) 800, 1093, 1273, 1875
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 7 cut(s) 166, 197, 373, 614, 1280, 1308, 2355
RsaNI GTAC 7 cut(s) 165, 196, 372, 613, 1279, 1307, 2354
RseI CAYNNNNRTG 3 cut(s) 218, 666, 1042
SacI GAGCTC 1 cut(s) 1036
SaqAI TTAA 6 cut(s) 414, 575, 995, 1235, 1590, 1811
SatI GCNGC 5 cut(s) 383, 839, 1134, 1207, 2080
Sau3AI GATC 9 cut(s) 460, 800, 806, 1093, 1273, 1875, 2037, 2146, 2233
Sau96I GGNCC 5 cut(s) 320, 505, 594, 902, 1783
ScaI AGTACT 1 cut(s) 373
SchI GAGTC 3 cut(s) 1326, 1796, 2167
ScrFI CCNGG 7 cut(s) 162, 389, 535, 555, 960, 1409, 1622
SduI GDGCHC 1 cut(s) 1036
SexAI ACCWGGT 1 cut(s) 1620
SfaNI GCATC 6 cut(s) 1202, 1282, 1336, 1653, 2180, 2202
Sfr274I CTCGAG 1 cut(s) 1776
SinI GGWCC 4 cut(s) 505, 594, 902, 1783
SlaI CTCGAG 1 cut(s) 1776
SmiMI CAYNNNNRTG 3 cut(s) 218, 666, 1042
SmlI CTYRAG 2 cut(s) 1776, 1804
SmoI CTYRAG 2 cut(s) 1776, 1804
SpeI ACTAGT 1 cut(s) 1435
SphI GCATGC 1 cut(s) 954
SsiI CCGC 6 cut(s) 178, 637, 838, 1209, 2080, 2395
SspI AATATT 1 cut(s) 1415
SspMI CTAG 7 cut(s) 821, 1436, 1472, 1511, 1839, 1892, 2033
SstI GAGCTC 1 cut(s) 1036
StyD4I CCNGG 7 cut(s) 160, 387, 533, 553, 958, 1407, 1620
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 3 cut(s) 1306, 2060, 2319
TaiI ACGT 3 cut(s) 1083, 1753, 1952
TaqI TCGA 2 cut(s) 316, 1777
TatI WGTACW 4 cut(s) 371, 612, 1278, 1306
TauI GCSGC 2 cut(s) 841, 2082
TfiI GAWTC 3 cut(s) 150, 392, 1856
Tru1I TTAA 6 cut(s) 414, 575, 995, 1235, 1590, 1811
Tru9I TTAA 6 cut(s) 414, 575, 995, 1235, 1590, 1811
TscAI CASTG 6 cut(s) 668, 934, 1189, 1450, 2050, 2065
TseFI GTSAC 6 cut(s) 110, 850, 881, 1300, 1962, 2060
TseI GCWGC 3 cut(s) 382, 1133, 1206
Tsp45I GTSAC 6 cut(s) 110, 850, 881, 1300, 1962, 2060
TspDTI ATGAA 7 cut(s) 293, 506, 1689, 1880, 2165, 2193, 2299
TspGWI ACGGA 3 cut(s) 813, 1215, 2151
TspRI CASTG 6 cut(s) 668, 934, 1189, 1450, 2050, 2065
Van91I CCANNNNNTGG 2 cut(s) 70, 560
VpaK11BI GGWCC 4 cut(s) 505, 594, 902, 1783
XapI RAATTY 2 cut(s) 1061, 2103
XceI RCATGY 4 cut(s) 476, 954, 1140, 2266
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XhoI CTCGAG 1 cut(s) 1776
XmiI GTMKAC 1 cut(s) 1507
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 7 cut(s) 821, 1436, 1472, 1511, 1839, 1892, 2033
ZrmI AGTACT 1 cut(s) 373
Zsp2I ATGCAT 4 cut(s) 6, 709, 1217, 2195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.