RchiOBHm_Chr5g0035421

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000099
Physical Location & Seq
Reverse (-)
29302448 .. 29306717
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31430

Sequence Viewer

Length: 2337 bp
ATGAGTTCCAGTATCGAAGCGTTTATCCAGATATTGCTTCTCTTCCTTCTTCTCCCCTCTAGCATTTGCCAGCTTCCCCTTGACGCCCTAACGCCAGACCGTCCCATCAGAGACGGCGACGTTTTAGTCTCTAACAAGCAAATCTTTGCACTAGGGTTCTTCAGCCTCGGAAATTCTCAGCACCGCTACGCTGGAGTTTGGTATAACAAGATTCCAGAGAAAGCCATTGTCTGGGTTGCGAACAGAGACAACCCAGTCAATGATTCCTATGGAATGCTATCGATCAATGGTGATGGAGGCCTTGTCATACATGGAAAGGACCGAAGTACGCCTCTTTGGTCCGCAAACGTTACTCTCTCTTCACCACACAATTTTACAGCCAAACTTTTGGATACAGGAAATCTTGTTTTACTTGAGAATGGTAGCCAAAGAGTTGTGTGGGAAGGCTTTGATTACCCATCAGATACACTGCTTCCCTCTATGAAGATTGGGCTGAACCGGCGGTCCGGGTTGGAGAAGCACATAACATCTTGGAAATCAAAAGATGACCCGGGAACCGGGAGTTGTACATATGGGATAGACCCGATGGGGGTTCCGCAGTCGTTTCTGCGCAAGGGTGGAGAACCATTGTGGCGGGGCGGCCCTTGGACTGGGCACAGATTGAGCGGTACCGTTGGAGACTATAATTCAAGTATTGTCAACAATGAAGACGAGATATCCTTCGTGTATTTGGACTCAGAGATCACAAGGGCGGTGATAGATGAATCAGGAATACTTCAAGGGTTCACGTGGAAGGATCAATGGATCAAATTCTACTCCTACCCGACTGGGTGGTGTGATTCCTACGGACGGTGCGGTCCAAATACTAACTGTGACCCAGGCAAGGACTATAAGTTGGCTTGCACGTGCCTACCTGCGTTCAAATTGAGTCTTGGTGCGGGTGGGTGCATTAGGAAAGCGGAAGCCTCCACATGTCAAAATGGAGAAGGGTTCGTGAAGGTGGCGAGTGTAAAAATACCGGACTCGTCTAAGGCACGTGTGAACATGAGCATGAGTTTGGAAATGTGCAGCCAAAAGTGCTTGATGGATTGTTCTTGCACAGCATTCACGAGTGCGGACGACCGGGGCGGTGGGATCGGGTGTATGACATGGCACGGGGACTTGATGGACACGAGGATTTTCTCCGATGTTGGTCAAGATTTATATGTTCGAGTCGATGCAACTTCTTTAGGTATGCGTAGGCAAAATAAATATTCATTTGAACTTACTGCTGGGTCAACCTACTTCGAAGAAGCTACTGGTGGATTAGTTCTTGATGATAGTAGAATAAACTCGGAGTTACTATTATTTCATCTAAACACCGTAGCCACCGCCACAAACAATTTCTCCATTGAAAACAAGCTTGGAGAAGGAGGGTTTGGCTCTGTTTATAAGGGGATACTTTACGATGGAAAAGAAATAGCCGTAAAAAGACTATCCAAGTTTTCTGGCCAAGGAATTGAAGAGTTTAAGAATGAAGTTCTTCTCATTGCAAAACTCCAACACAGAAACCTTGTTAAGATTTTAGGTTGTTGTTTTGAAGGTGAAGAGAAGATTCTAATCTATGAATACTTACCAAACAAAAGTTTGGACTGTTTCATCTTTAATGAAACAAGGAGAGCACTTTTAAGTTGGACAAGACGCTCCGAGATTATCTTGGGGATTGCTAGAGGGTTATTATATCTTCATGAAGATTCAAGACTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTTCTATTGGATAATTCTTTGAACCCAAAGATTGCAGATTTTGGTATAGCTAGATTATTTAGAGGGGAGCAAACTGAAGCAAATACAAATCGTGTGGTTGGAACATATGGTTATATGTCACCGGAATATGCAATGGAAGGACTGTTTTCAATAAAGTCTGATGTGTATAGTTTTGGTGTTTTATTGCTAGAAATCATTACTGGCAAAAAGAATGCTGGTTCCTATGAGAAGTATCCATATTCAAATTTGATTGGACATGTTTGGGAGTTGTGGAAAGAAGGCAGAGCCGTGGAAATCATTGATTCATCTATAGGTGAATCTTACCTTGTTAGTGAAATAATAAGGTACATTCAAATCGCACTCCTGTGTGTGCAAGAATTTGCGACTGACCGGCCAACCATGTCGGTAGTCGTTTCAATGTTAAGTAATGATGCAGCTCTTCCTTCACCGAGACGACCCGCATTTTTTCTGAAGACAATGAGTCCTAGTGGAGACCCATCCAGCAGCAGAGAAGTTTGTTCAGTAAACGGTGTCACATGTACAATTGTAGAAGCTCGCTAA

Protein Analysis

778

Amino Acids

86.11

Weight (kDa)

5.9

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 179 8.6e-33 D-mannose binding lectin
S_locus_glycop PF00954 211 - 308 2.4e-14 S-locus glycoprotein domain
PAN_2 PF08276 325 - 391 1.7e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 463 - 731 2.9e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 463 - 665 1.4e-42 Protein kinase domain
DUF3403 PF11883 734 - 778 5.5e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1431
AasI GACNNNNNNGTC 3 cut(s) 125, 254, 855
Acc16I TGCGCA 1 cut(s) 611
Acc36I ACCTGC 1 cut(s) 922
Acc65I GGTACC 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 668
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 666
AclI AACGTT 1 cut(s) 348
AclWI GGATC 3 cut(s) 804, 812, 1142
AcoI YGGCCR 2 cut(s) 1489, 2168
AcsI RAATTY 4 cut(s) 172, 809, 2020, 2153
AcuI CTGAAG 3 cut(s) 145, 1872, 2267
AcvI CACGTG 3 cut(s) 789, 906, 1037
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 5 cut(s) 328, 568, 670, 2123, 2317
AfiI CCNNNNNNNGG 6 cut(s) 231, 557, 589, 650, 849, 883
AflIII ACRYGT 4 cut(s) 971, 1036, 2032, 2312
AhdI GACNNNNNGTC 1 cut(s) 2256
AjnI CCWGG 1 cut(s) 877
AjuI GAANNNNNNNTTGG 4 cut(s) 1386, 1401, 1418, 1433
AleI CACNNNNGTG 1 cut(s) 2140
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
AluBI AGCT 6 cut(s) 73, 1295, 1402, 1826, 2213, 2330
AluI AGCT 6 cut(s) 73, 1295, 1402, 1826, 2213, 2330
Alw21I GWGCWC 1 cut(s) 1663
Alw26I GTCTC 6 cut(s) 105, 133, 240, 672, 2221, 2262
AlwI GGATC 3 cut(s) 804, 812, 1142
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 5 cut(s) 298, 640, 1489, 1767, 2168
ApeKI GCWGC 3 cut(s) 1068, 2210, 2280
ApoI RAATTY 4 cut(s) 172, 809, 2020, 2153
Asp700I GAANNNNTTC 1 cut(s) 1521
Asp718I GGTACC 1 cut(s) 668
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 5 cut(s) 319, 339, 504, 641, 857
AsuC2I CCSGG 5 cut(s) 508, 551, 552, 559, 1124
AsuHPI GGTGA 7 cut(s) 302, 354, 766, 1595, 1887, 2102, 2214
AsuII TTCGAA 1 cut(s) 1287
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 4 cut(s) 319, 339, 504, 857
BaeGI GKGCMC 1 cut(s) 657
BalI TGGCCA 1 cut(s) 1491
BanI GGYRCC 1 cut(s) 668
BauI CACGAG 2 cut(s) 1108, 1171
BbrPI CACGTG 3 cut(s) 789, 906, 1037
BbsI GAAGAC 2 cut(s) 714, 2255
Bbv12I GWGCWC 1 cut(s) 1663
BbvI GCAGC 3 cut(s) 1080, 2222, 2292
BccI CCATC 8 cut(s) 113, 287, 466, 580, 1078, 1159, 1442, 2281
BceAI ACGGC 3 cut(s) 130, 1448, 2048
BciT130I CCWGG 1 cut(s) 879
BciVI GTATCC 3 cut(s) 385, 1431, 2019
BcnI CCSGG 5 cut(s) 508, 551, 552, 559, 1124
BcoDI GTCTC 6 cut(s) 105, 133, 240, 672, 2221, 2262
BfaI CTAG 6 cut(s) 60, 152, 1707, 1827, 1964, 2262
BfmI CTRYAG 1 cut(s) 2085
BfuAI ACCTGC 1 cut(s) 922
BfuI GTATCC 3 cut(s) 385, 1431, 2019
BglII AGATCT 1 cut(s) 1759
BisI GCNGC 4 cut(s) 640, 1069, 2211, 2281
BlsI GCNGC 4 cut(s) 641, 1070, 2212, 2282
Bme1390I CCNGG 6 cut(s) 508, 551, 552, 559, 879, 1124
Bme18I GGWCC 4 cut(s) 319, 339, 504, 857
BmeRI GACNNNNNGTC 1 cut(s) 2256
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 5 cut(s) 319, 339, 504, 641, 857
BmiI GGNNCC 4 cut(s) 556, 594, 670, 1996
BmrFI CCNGG 6 cut(s) 508, 551, 552, 559, 879, 1124
BmrI ACTGGG 3 cut(s) 248, 660, 837
BmsI GCATC 2 cut(s) 1207, 2197
BmuI ACTGGG 3 cut(s) 248, 660, 837
BpiI GAAGAC 2 cut(s) 714, 2255
BpmI CTGGAG 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 1287
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 5 cut(s) 508, 551, 552, 559, 1124
Bsa29I ATCGAT 1 cut(s) 281
BsaAI YACGTR 3 cut(s) 789, 906, 1037
BsaBI GATNNNNATC 1 cut(s) 1598
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 2262
BsaJI CCNNGG 7 cut(s) 166, 550, 644, 877, 1123, 1492, 2064
BsaWI WCCGGW 2 cut(s) 1018, 1897
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 1370, 1400
Bsc4I CCNNNNNNNGG 6 cut(s) 231, 557, 589, 650, 849, 883
Bse118I RCCGGY 2 cut(s) 498, 2166
Bse1I ACTGG 6 cut(s) 9, 254, 655, 832, 1303, 1981
Bse3DI GCAATG 3 cut(s) 1527, 1780, 1914
Bse8I GATNNNNATC 1 cut(s) 1598
BseBI CCWGG 1 cut(s) 879
BseCI ATCGAT 1 cut(s) 281
BseDI CCNNGG 7 cut(s) 166, 550, 644, 877, 1123, 1492, 2064
BseGI GGATG 1 cut(s) 2273
BseJI GATNNNNATC 1 cut(s) 1598
BseLI CCNNNNNNNGG 6 cut(s) 231, 557, 589, 650, 849, 883
BseMI GCAATG 3 cut(s) 1527, 1780, 1914
BseMII CTCAG 2 cut(s) 191, 750
BseNI ACTGG 6 cut(s) 9, 254, 655, 832, 1303, 1981
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 3 cut(s) 1080, 2222, 2292
BseYI CCCAGC 1 cut(s) 1271
BsgI GTGCAG 1 cut(s) 1087
Bsh1285I CGRYCG 1 cut(s) 1123
BshFI GGCC 5 cut(s) 300, 642, 1491, 1769, 2170
BshNI GGYRCC 1 cut(s) 668
BshVI ATCGAT 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 1123
BsiHKAI GWGCWC 1 cut(s) 1663
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 8 cut(s) 499, 507, 551, 558, 1019, 1123, 1898, 2167
BslFI GGGAC 2 cut(s) 87, 1172
BslI CCNNNNNNNGG 6 cut(s) 231, 557, 589, 650, 849, 883
BsmAI GTCTC 6 cut(s) 105, 133, 240, 672, 2221, 2262
BsmBI CGTCTC 2 cut(s) 105, 2221
BsmFI GGGAC 2 cut(s) 87, 1172
BsmI GAATGC 3 cut(s) 279, 1103, 1993
BsnI GGCC 5 cut(s) 300, 642, 1491, 1769, 2170
Bso31I GGTCTC 1 cut(s) 2262
BsoBI CYCGRG 1 cut(s) 550
Bsp119I TTCGAA 1 cut(s) 1287
Bsp1286I GDGCHC 2 cut(s) 657, 1663
Bsp1407I TGTACA 2 cut(s) 566, 2315
Bsp143I GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
BspANI GGCC 5 cut(s) 300, 642, 1491, 1769, 2170
BspCNI CTCAG 2 cut(s) 190, 749
BspDI ATCGAT 1 cut(s) 281
BspHI TCATGA 1 cut(s) 1726
BspLI GGNNCC 4 cut(s) 556, 594, 670, 1996
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 3 cut(s) 804, 812, 1142
BspQI GCTCTTC 1 cut(s) 2220
BspT104I TTCGAA 1 cut(s) 1287
BspT107I GGYRCC 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 2262
BsrBI CCGCTC 1 cut(s) 666
BsrDI GCAATG 3 cut(s) 1527, 1780, 1914
BsrFI RCCGGY 2 cut(s) 498, 2166
BsrGI TGTACA 2 cut(s) 566, 2315
BsrI ACTGG 6 cut(s) 9, 254, 655, 832, 1303, 1981
BssAI RCCGGY 2 cut(s) 498, 2166
BssECI CCNNGG 7 cut(s) 166, 550, 644, 877, 1123, 1492, 2064
BssMI GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 2 cut(s) 1108, 1171
BssT1I CCWWGG 2 cut(s) 644, 1492
Bst2BI CACGAG 2 cut(s) 1108, 1171
Bst2UI CCWGG 1 cut(s) 879
Bst4CI ACNGT 8 cut(s) 101, 673, 852, 872, 1363, 1634, 1920, 2306
Bst6I CTCTTC 5 cut(s) 47, 364, 1497, 1581, 2220
BstACI GRCGYC 1 cut(s) 84
BstAUI TGTACA 2 cut(s) 566, 2315
BstBAI YACGTR 3 cut(s) 789, 906, 1037
BstBI TTCGAA 1 cut(s) 1287
BstC8I GCNNGC 4 cut(s) 71, 901, 1771, 2332
BstDEI CTNAG 4 cut(s) 177, 736, 1029, 1742
BstDSI CCRYGG 1 cut(s) 2064
BstF5I GGATG 1 cut(s) 2273
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 6 cut(s) 285, 744, 799, 807, 1137, 1762
BstMAI GTCTC 6 cut(s) 105, 133, 240, 672, 2221, 2262
BstMBI GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
BstMCI CGRYCG 1 cut(s) 1123
BstMWI GCNNNNNNNGC 2 cut(s) 499, 1077
BstNI CCWGG 1 cut(s) 879
BstNSI RCATGY 3 cut(s) 975, 2036, 2316
BstSCI CCNGG 6 cut(s) 506, 549, 550, 557, 877, 1122
BstSFI CTRYAG 1 cut(s) 2085
BstSLI GKGCMC 1 cut(s) 657
BstV1I GCAGC 3 cut(s) 1080, 2222, 2292
BstV2I GAAGAC 2 cut(s) 714, 2255
BstX2I RGATCY 1 cut(s) 1759
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 1 cut(s) 1759
Bsu15I ATCGAT 1 cut(s) 281
BsuI GTATCC 3 cut(s) 385, 1431, 2019
BsuRI GGCC 5 cut(s) 300, 642, 1491, 1769, 2170
BsuTUI ATCGAT 1 cut(s) 281
BtgI CCRYGG 1 cut(s) 2064
BtsCI GGATG 1 cut(s) 2273
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 1 cut(s) 467
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 4 cut(s) 71, 901, 1771, 2332
CciI TCATGA 1 cut(s) 1726
CfoI GCGC 1 cut(s) 612
Cfr10I RCCGGY 2 cut(s) 498, 2166
Cfr13I GGNCC 5 cut(s) 319, 339, 504, 641, 857
Cfr9I CCCGGG 1 cut(s) 550
ClaI ATCGAT 1 cut(s) 281
CpoI CGGWCCG 1 cut(s) 504
CseI GACGC 2 cut(s) 92, 1689
Csp6I GTAC 5 cut(s) 327, 567, 669, 2122, 2316
CspCI CAANNNNNGTGG 2 cut(s) 1851, 1886
CspI CGGWCCG 1 cut(s) 504
CviAII CATG 9 cut(s) 311, 972, 1045, 1051, 1149, 1727, 2033, 2176, 2313
CviQI GTAC 5 cut(s) 327, 567, 669, 2122, 2316
DdeI CTNAG 4 cut(s) 177, 736, 1029, 1742
DpnI GATC 6 cut(s) 284, 743, 798, 806, 1136, 1761
DpnII GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
DrdI GACNNNNNNGTC 3 cut(s) 125, 254, 855
DriI GACNNNNNGTC 1 cut(s) 2256
DseDI GACNNNNNNGTC 3 cut(s) 125, 254, 855
EaeI YGGCCR 2 cut(s) 1489, 2168
Eam1104I CTCTTC 5 cut(s) 47, 364, 1497, 1581, 2220
Eam1105I GACNNNNNGTC 1 cut(s) 2256
EarI CTCTTC 5 cut(s) 47, 364, 1497, 1581, 2220
Eco130I CCWWGG 2 cut(s) 644, 1492
Eco147I AGGCCT 1 cut(s) 300
Eco31I GGTCTC 1 cut(s) 2262
Eco32I GATATC 1 cut(s) 717
Eco47I GGWCC 4 cut(s) 319, 339, 504, 857
Eco57I CTGAAG 3 cut(s) 145, 1872, 2267
Eco72I CACGTG 3 cut(s) 789, 906, 1037
Eco88I CYCGRG 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 877
EcoRV GATATC 1 cut(s) 717
EcoT14I CCWWGG 2 cut(s) 644, 1492
ErhI CCWWGG 2 cut(s) 644, 1492
Esp3I CGTCTC 2 cut(s) 105, 2221
FaeI CATG 9 cut(s) 314, 975, 1048, 1054, 1152, 1730, 2036, 2179, 2316
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 2 cut(s) 87, 1172
FatI CATG 9 cut(s) 310, 971, 1044, 1050, 1148, 1726, 2032, 2175, 2312
FauI CCCGC 3 cut(s) 627, 931, 2242
FauNDI CATATG 2 cut(s) 571, 1882
Fnu4HI GCNGC 4 cut(s) 640, 1069, 2211, 2281
FokI GGATG 1 cut(s) 2260
Fsp4HI GCNGC 4 cut(s) 640, 1069, 2211, 2281
FspBI CTAG 6 cut(s) 60, 152, 1707, 1827, 1964, 2262
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 1 cut(s) 611
GluI GCNGC 4 cut(s) 640, 1069, 2211, 2281
GsaI CCCAGC 1 cut(s) 1275
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 5 cut(s) 300, 642, 1491, 1769, 2170
HapII CCGG 8 cut(s) 499, 507, 551, 558, 1019, 1123, 1898, 2167
HgaI GACGC 2 cut(s) 92, 1689
HhaI GCGC 1 cut(s) 612
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 9 cut(s) 314, 975, 1048, 1054, 1152, 1730, 2036, 2179, 2316
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HincII GTYRAC 2 cut(s) 700, 1278
HindII GTYRAC 2 cut(s) 700, 1278
HindIII AAGCTT 1 cut(s) 1400
HpaII CCGG 8 cut(s) 499, 507, 551, 558, 1019, 1123, 1898, 2167
HphI GGTGA 7 cut(s) 302, 354, 766, 1595, 1887, 2102, 2214
Hpy166II GTNNAC 5 cut(s) 700, 786, 1042, 1278, 2302
Hpy188I TCNGA 9 cut(s) 110, 170, 463, 739, 1186, 1336, 1687, 1936, 2247
Hpy188III TCNNGA 9 cut(s) 28, 215, 768, 994, 1108, 1196, 1313, 1727, 1737
Hpy8I GTNNAC 5 cut(s) 700, 786, 1042, 1278, 2302
Hpy99I CGWCG 1 cut(s) 122
HpyCH4III ACNGT 8 cut(s) 101, 673, 852, 872, 1363, 1634, 1920, 2306
HpyCH4IV ACGT 5 cut(s) 120, 348, 788, 905, 1036
HpyF10VI GCNNNNNNNGC 2 cut(s) 499, 1077
HpyF3I CTNAG 4 cut(s) 177, 736, 1029, 1742
HpySE526I ACGT 5 cut(s) 120, 348, 788, 905, 1036
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 9 cut(s) 314, 975, 1048, 1054, 1152, 1730, 2036, 2179, 2316
HspAI GCGC 1 cut(s) 610
KpnI GGTACC 1 cut(s) 672
Kzo9I GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
LguI GCTCTTC 1 cut(s) 2220
LmnI GCTCC 2 cut(s) 1688, 1843
Lsp1109I GCAGC 3 cut(s) 1080, 2222, 2292
LweI GCATC 2 cut(s) 1207, 2197
MaeI CTAG 6 cut(s) 60, 152, 1707, 1827, 1964, 2262
MaeII ACGT 5 cut(s) 120, 348, 788, 905, 1036
MaeIII GTNAC 5 cut(s) 349, 872, 1338, 1893, 2308
MalI GATC 6 cut(s) 284, 743, 798, 806, 1136, 1761
MbiI CCGCTC 1 cut(s) 666
MboI GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
MfeI CAATTG 1 cut(s) 2319
MflI RGATCY 1 cut(s) 1759
MhlI GDGCHC 2 cut(s) 657, 1663
MlsI TGGCCA 1 cut(s) 1491
MluNI TGGCCA 1 cut(s) 1491
MlyI GAGTC 5 cut(s) 728, 937, 1016, 1221, 2266
MmeI TCCRAC 5 cut(s) 492, 655, 1564, 1652, 1855
Mox20I TGGCCA 1 cut(s) 1491
MroXI GAANNNNTTC 1 cut(s) 1521
MscI TGGCCA 1 cut(s) 1491
MseI TTAA 5 cut(s) 1509, 1557, 1644, 1667, 2198
MslI CAYNNNNRTG 2 cut(s) 1049, 2140
Msp20I TGGCCA 1 cut(s) 1491
MspI CCGG 8 cut(s) 499, 507, 551, 558, 1019, 1123, 1898, 2167
MspR9I CCNGG 6 cut(s) 508, 551, 552, 559, 879, 1124
MunI CAATTG 1 cut(s) 2319
Mva1269I GAATGC 3 cut(s) 279, 1103, 1993
MvaI CCWGG 1 cut(s) 879
MwoI GCNNNNNNNGC 2 cut(s) 499, 1077
NciI CCSGG 5 cut(s) 508, 551, 552, 559, 1124
NdeI CATATG 2 cut(s) 571, 1882
NdeII GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
NlaIII CATG 9 cut(s) 314, 975, 1048, 1054, 1152, 1730, 2036, 2179, 2316
NlaIV GGNNCC 4 cut(s) 556, 594, 670, 1996
NmuCI GTSAC 3 cut(s) 872, 1893, 2308
NsbI TGCGCA 1 cut(s) 611
NspI RCATGY 3 cut(s) 975, 2036, 2316
NspV TTCGAA 1 cut(s) 1287
OliI CACNNNNGTG 1 cut(s) 2140
PagI TCATGA 1 cut(s) 1726
PceI AGGCCT 1 cut(s) 300
PciI ACATGT 3 cut(s) 971, 2032, 2312
PciSI GCTCTTC 1 cut(s) 2220
PctI GAATGC 3 cut(s) 279, 1103, 1993
PdmI GAANNNNTTC 1 cut(s) 1521
PfeI GAWTC 8 cut(s) 211, 263, 764, 839, 1594, 1733, 2078, 2093
PflMI CCANNNNNTGG 1 cut(s) 231
PkrI GCNGC 4 cut(s) 641, 1070, 2212, 2282
PleI GAGTC 5 cut(s) 728, 936, 1016, 1220, 2265
PmaCI CACGTG 3 cut(s) 789, 906, 1037
PmlI CACGTG 3 cut(s) 789, 906, 1037
PpsI GAGTC 5 cut(s) 728, 936, 1016, 1220, 2265
Ppu21I YACGTR 3 cut(s) 789, 906, 1037
PscI ACATGT 3 cut(s) 971, 2032, 2312
PsiI TTATAA 1 cut(s) 1431
Psp1406I AACGTT 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 877
PspCI CACGTG 3 cut(s) 789, 906, 1037
PspFI CCCAGC 1 cut(s) 1271
PspGI CCWGG 1 cut(s) 877
PspN4I GGNNCC 4 cut(s) 556, 594, 670, 1996
PspPI GGNCC 5 cut(s) 319, 339, 504, 641, 857
PsuI RGATCY 1 cut(s) 1759
RsaI GTAC 5 cut(s) 328, 568, 670, 2123, 2317
RsaNI GTAC 5 cut(s) 327, 567, 669, 2122, 2316
RseI CAYNNNNRTG 2 cut(s) 1049, 2140
Rsr2I CGGWCCG 1 cut(s) 504
RsrII CGGWCCG 1 cut(s) 504
SapI GCTCTTC 1 cut(s) 2220
SaqAI TTAA 5 cut(s) 1509, 1557, 1644, 1667, 2198
SatI GCNGC 4 cut(s) 640, 1069, 2211, 2281
Sau3AI GATC 6 cut(s) 282, 741, 796, 804, 1134, 1759
Sau96I GGNCC 5 cut(s) 319, 339, 504, 641, 857
SchI GAGTC 5 cut(s) 728, 937, 1016, 1221, 2266
ScrFI CCNGG 6 cut(s) 508, 551, 552, 559, 879, 1124
SduI GDGCHC 2 cut(s) 657, 1663
SfaNI GCATC 2 cut(s) 1207, 2197
SfcI CTRYAG 1 cut(s) 2085
SfuI TTCGAA 1 cut(s) 1287
SinI GGWCC 4 cut(s) 319, 339, 504, 857
SmaI CCCGGG 1 cut(s) 552
SmiMI CAYNNNNRTG 2 cut(s) 1049, 2140
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SseBI AGGCCT 1 cut(s) 300
SspI AATATT 1 cut(s) 1253
SspMI CTAG 6 cut(s) 60, 152, 1707, 1827, 1964, 2262
StuI AGGCCT 1 cut(s) 300
StyD4I CCNGG 6 cut(s) 506, 549, 550, 557, 877, 1122
StyI CCWWGG 2 cut(s) 644, 1492
TaaI ACNGT 8 cut(s) 101, 673, 852, 872, 1363, 1634, 1920, 2306
TaiI ACGT 5 cut(s) 123, 351, 791, 908, 1039
TaqI TCGA 5 cut(s) 15, 281, 1210, 1215, 1287
TaqII GACCGA 1 cut(s) 336
TatI WGTACW 2 cut(s) 566, 2315
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 8 cut(s) 211, 263, 764, 839, 1594, 1733, 2078, 2093
Tru1I TTAA 5 cut(s) 1509, 1557, 1644, 1667, 2198
Tru9I TTAA 5 cut(s) 1509, 1557, 1644, 1667, 2198
TscAI CASTG 1 cut(s) 474
TseFI GTSAC 3 cut(s) 872, 1893, 2308
TseI GCWGC 3 cut(s) 1068, 2210, 2280
Tsp45I GTSAC 3 cut(s) 872, 1893, 2308
TspGWI ACGGA 1 cut(s) 861
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 1 cut(s) 474
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 4 cut(s) 319, 339, 504, 857
XapI RAATTY 4 cut(s) 172, 809, 2020, 2153
XceI RCATGY 3 cut(s) 975, 2036, 2316
XmaI CCCGGG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 1521
XspI CTAG 6 cut(s) 60, 152, 1707, 1827, 1964, 2262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.