RchiOBHm_Chr5g0035471

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000099
Physical Location & Seq
Reverse (-)
29376631 .. 29383624
6994 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31433

Sequence Viewer

Length: 2475 bp
ATGATCGAATCGTTTATCCAGATATTCCTTCTCTTCCTTCTTCTCCCCTCTAGCATTTGCCAGCTTCCCCTTGACGCCCTTACGCCAGACCGTCCCATCAGAGACGGCGACGTTTTAGTCTCTAACAAGCAAATCTTTGCACTAGGATTCTTCAGCCCCGGAAATTCTCAGCACCGCTACGCTGGAGTTTGGTATAACAACATTCCAGAGAAAGCCATTGTCTGGGTTGCGAACAGAGACAACCCAGTCAATGATTCCTACGGAGTGCTATCCATCAACGGTGATGGAGGCCTTGTCATACATGGGAAGGACCCAAGTACCCCTCTTTGGTCCGCAAACGTTACTCTCTCTTCACCAAACAATTTCACAGCCAAGCTTTTGGATACAGGAAATCTTGTTTTACTTGAGAATAGCAGCCAAAGAGTTGTGTGGGAAGGCTTTGATTACCCCTCAGATACACTGCTTCCCTTCATGAAGATTGGGCTGAACCAGCGGTCCGGGTTGGAGAAGCACCTAACATCTTGGAAATCAAAAGATGACCCGGGAACCGGGAGTTGTACATATGGGATAGACCCGATGGGGGTTCCGCAGTTGTTTGTGCGCAAGGGTAGAGACGCATTGTTTCGGGTCGGCCCTTGGACTGGGGACAGATTGAGCGGTACCGTTGGAAACTACAATTCAAGTTTTGTCAACAATGAAGACGAGATATCCTTCGTGTATTTTCCTGCTCTGAGGCCTCAGATCGCAAGGGTGGTGATAGATGAATCAGGAATACTTCAAACGTTCGCGTGGAATGATCAATGGATCAAATCCTACTCCTACCCGACTAGGTGGTGTGATTCCTACGGACAGTGCGGTCCAAATACTAACTGTGACCCAGACAAGGACTATAAGTTGGCTTGCACGTGCCTACCTGGGTTCGAATCCAAATCACCTAGTTTGGGTCTTGGTGAGGGTGCATGCATTAGGAAAGCGGAAGTGTCCACATGTCAAAATGGAGAAGGGTTCGTGAAGGTGGCGCGTGTAAACATACCGGACTCGTCTACGGCACGTGTGAACCTGAGTATGAGTTTGGAACAGTGCAAACAAAAGTGCTTGATGGATTGCTCTTGCACAGCTTACACGAGTGCGGATGACCGGGGAGGTGGGATTGGGTGTGTGACATGGCAGGGGGACTTGATAGACACGAAGACTTTCTCTCATGTTGGTCAAGATTTATACGTTCGAGTCGATGCAACTTTTTTAGCTCAATATGCAAAGTCAAGTGGTTCTCTTAGCAAGAAGGCAAGGGTGGCAATTTCACTTGGATCTGTTACAGTTTTTATTCTCTTACTTACCCTTTATTGGTTGGTAAGGATGAAGATGAAGGGTAAGCGTAGGCAAAACAAATGTTCATTCGAACTTACTGCTGGGTCAACCTACTTCGAAGAAGCTACTAGTGGATTAATTCTTGATGATAGTAGAATAAACTCGGAGTTACTATTATTTCATCTAAACACCGTAGCCACTGCCACAAACAATTTCTCCATTGAAAACAAGCTTGGAGAAGGAGGGTTTGGCTCCGTTTATAAGGGGATGCTTTACGATGGAAAGGAAATAGCTGTAAAAAGACTATCCAAGTTTTCTAGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTGATTGCAAAACTCCAACACAGAAACCTTGTTAAGATTTTAGGTTGCTGTTTTGAAGATGAAGAGAAGATTTTAATATATGAATACTTGCCAAACAAAAGTTTGGACTCTTTCATCTTTAATGAACCAAAGAGAGCACTTTTAAGTTGGACAAGACGCTTCGAGATTATCTTGGGGATTGCTAGAGGGCTATTATATCTTCATGAAGATTCAAGACTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTTCTATTGGATAATTCTTTGAACCCAAAGATTGCAGATTTTGGTATGGCTAGAATATTTAGAGGAGAGCAAACTGAAGCAAACACAAATCATGTGGTTGGAACATATGGTTATATGTCACCAGAATATGCAATGAAAGGGCTGTTTTCAATAAAGTCTGATGTGTATAGTTTTGGTATTTTATTGCTAGAAATCATTACTGGCAAAAAGAATGCTGGTTCCTTTGAGAAGGATCCATATTCAAATTTGATTGGACATGTTTGGGAGTTATGGAAAGAAGGCAGAGCCGTGGAAATCATTGATTCATCTATAGGTGAATCTTACCTTGTTAGTGAAATTATAAGGTGCATTCAAATCGCACTCCTGTGTGTGCAAGAATTTGCGATTGACCGGCCAACCATGTCGGTAGTTGTTTCAATGTTAAGTAATGATGTAGCTTTTACTTCACCAAAACGACCTGCATTTTTACTAAAGAGCATGAGTCCTAGTGGAGAACCATCCAGCAACAAAAGAGTTAGTTCAATAAATGATGTCACATGTACAATTGTAGAAGCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

91.54

Weight (kDa)

6.59

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 176 4e-33 D-mannose binding lectin
S_locus_glycop PF00954 208 - 310 3.7e-17 S-locus glycoprotein domain
PAN_2 PF08276 330 - 396 1.4e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 509 - 713 2.8e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 509 - 711 2.2e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1569, 2255
AasI GACNNNNNNGTC 3 cut(s) 116, 245, 855
Acc16I TGCGCA 1 cut(s) 602
Acc36I ACCTGC 1 cut(s) 2380
Acc65I GGTACC 1 cut(s) 659
AccB1I GGYRCC 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 222
AccBSI CCGCTC 1 cut(s) 657
AccI GTMKAC 1 cut(s) 1043
AccII CGCG 2 cut(s) 788, 1021
AciI CCGC 8 cut(s) 175, 333, 493, 587, 657, 855, 974, 1130
AclI AACGTT 2 cut(s) 339, 782
AclWI GGATC 4 cut(s) 812, 1315, 2141, 2154
AcoI YGGCCR 1 cut(s) 2306
AcsI RAATTY 3 cut(s) 163, 2158, 2291
AcuI CTGAAG 2 cut(s) 136, 2010
AcvI CACGTG 2 cut(s) 906, 1052
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 4 cut(s) 319, 559, 661, 2455
AfiI CCNNNNNNNGG 8 cut(s) 222, 327, 548, 580, 641, 883, 941, 1344
AflIII ACRYGT 4 cut(s) 986, 1051, 2170, 2450
AhlI ACTAGT 1 cut(s) 1436
AjnI CCWGG 1 cut(s) 913
AjuI GAANNNNNNNTTGG 4 cut(s) 1524, 1539, 1556, 1571
AleI CACNNNNGTG 1 cut(s) 2278
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 9 cut(s) 64, 376, 1118, 1247, 1433, 1540, 1601, 2351, 2468
AluI AGCT 9 cut(s) 64, 376, 1118, 1247, 1433, 1540, 1601, 2351, 2468
Alw21I GWGCWC 1 cut(s) 1801
Alw26I GTCTC 4 cut(s) 96, 124, 231, 606
AlwI GGATC 4 cut(s) 812, 1315, 2141, 2154
Ama87I CYCGRG 1 cut(s) 541
AoxI GGCC 5 cut(s) 289, 631, 734, 1905, 2306
ApeKI GCWGC 1 cut(s) 414
ApoI RAATTY 3 cut(s) 163, 2158, 2291
AseI ATTAAT 1 cut(s) 1445
Asp700I GAANNNNTTC 2 cut(s) 1193, 1659
Asp718I GGTACC 1 cut(s) 659
AspLEI GCGC 2 cut(s) 603, 1021
AspS9I GGNCC 5 cut(s) 310, 330, 495, 632, 857
AsuC2I CCSGG 6 cut(s) 159, 499, 542, 543, 550, 1139
AsuHPI GGTGA 8 cut(s) 293, 345, 766, 924, 962, 2025, 2240, 2352
AsuII TTCGAA 3 cut(s) 921, 1398, 1425
AvaI CYCGRG 1 cut(s) 541
AvaII GGWCC 4 cut(s) 310, 330, 495, 857
BaeI ACNNNNGTAYC 2 cut(s) 301, 334
BamHI GGATCC 1 cut(s) 2146
BanI GGYRCC 1 cut(s) 659
BauI CACGAG 1 cut(s) 1123
BbrPI CACGTG 2 cut(s) 906, 1052
BbsI GAAGAC 2 cut(s) 705, 1196
Bbv12I GWGCWC 1 cut(s) 1801
BbvI GCAGC 1 cut(s) 426
BccI CCATC 7 cut(s) 104, 278, 281, 571, 1093, 1580, 2419
BceAI ACGGC 3 cut(s) 121, 1062, 2186
BcgI CGANNNNNNTGC 4 cut(s) 1388, 1422, 2251, 2285
BciT130I CCWGG 1 cut(s) 915
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 6 cut(s) 159, 499, 542, 543, 550, 1139
BcoDI GTCTC 4 cut(s) 96, 124, 231, 606
BcuI ACTAGT 1 cut(s) 1436
BfmI CTRYAG 1 cut(s) 2223
BfuAI ACCTGC 1 cut(s) 2380
BfuI GTATCC 1 cut(s) 376
BglII AGATCT 1 cut(s) 1897
BisI GCNGC 1 cut(s) 415
BlsI GCNGC 1 cut(s) 416
Bme1390I CCNGG 7 cut(s) 159, 499, 542, 543, 550, 915, 1139
Bme18I GGWCC 4 cut(s) 310, 330, 495, 857
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 5 cut(s) 310, 330, 495, 632, 857
BmiI GGNNCC 7 cut(s) 312, 547, 585, 661, 1561, 2134, 2148
BmrFI CCNGG 7 cut(s) 159, 499, 542, 543, 550, 915, 1139
BmrI ACTGGG 2 cut(s) 239, 651
BmsI GCATC 2 cut(s) 1222, 1566
BmuI ACTGGG 2 cut(s) 239, 651
BpiI GAAGAC 2 cut(s) 705, 1196
BpmI CTGGAG 1 cut(s) 204
Bpu14I TTCGAA 3 cut(s) 921, 1398, 1425
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 6 cut(s) 159, 499, 542, 543, 550, 1139
BsaAI YACGTR 2 cut(s) 906, 1052
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 7 cut(s) 157, 541, 635, 914, 1138, 1630, 2202
BsaWI WCCGGW 1 cut(s) 1033
BsaXI ACNNNNNCTCC 4 cut(s) 279, 309, 1508, 1538
Bsc4I CCNNNNNNNGG 8 cut(s) 222, 327, 548, 580, 641, 883, 941, 1344
Bse118I RCCGGY 1 cut(s) 2304
Bse1I ACTGG 3 cut(s) 245, 646, 2119
Bse3DI GCAATG 2 cut(s) 1918, 2052
BseBI CCWGG 1 cut(s) 915
BseDI CCNNGG 7 cut(s) 157, 541, 635, 914, 1138, 1630, 2202
BseGI GGATG 4 cut(s) 1138, 1362, 1581, 2411
BseLI CCNNNNNNNGG 8 cut(s) 222, 327, 548, 580, 641, 883, 941, 1344
BseMI GCAATG 2 cut(s) 1918, 2052
BseMII CTCAG 5 cut(s) 182, 465, 722, 752, 1052
BseNI ACTGG 3 cut(s) 245, 646, 2119
BseRI GAGGAG 1 cut(s) 1992
BseXI GCAGC 1 cut(s) 426
BseYI CCCAGC 1 cut(s) 1409
Bsh1236I CGCG 2 cut(s) 788, 1021
BshFI GGCC 5 cut(s) 291, 633, 736, 1907, 2308
BshNI GGYRCC 1 cut(s) 659
BsiHKAI GWGCWC 1 cut(s) 1801
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 7 cut(s) 159, 498, 542, 549, 1034, 1138, 2305
BslFI GGGAC 3 cut(s) 78, 659, 1187
BslI CCNNNNNNNGG 8 cut(s) 222, 327, 548, 580, 641, 883, 941, 1344
BsmAI GTCTC 4 cut(s) 96, 124, 231, 606
BsmBI CGTCTC 2 cut(s) 96, 606
BsmFI GGGAC 3 cut(s) 78, 659, 1187
BsmI GAATGC 2 cut(s) 2131, 2262
BsnI GGCC 5 cut(s) 291, 633, 736, 1907, 2308
BsoBI CYCGRG 1 cut(s) 541
Bsp119I TTCGAA 3 cut(s) 921, 1398, 1425
Bsp1286I GDGCHC 1 cut(s) 1801
Bsp1407I TGTACA 2 cut(s) 557, 2453
Bsp143I GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
BspACI CCGC 8 cut(s) 175, 333, 493, 587, 657, 855, 974, 1130
BspANI GGCC 5 cut(s) 291, 633, 736, 1907, 2308
BspCNI CTCAG 5 cut(s) 181, 464, 723, 751, 1053
BspFNI CGCG 2 cut(s) 788, 1021
BspHI TCATGA 2 cut(s) 471, 1864
BspLI GGNNCC 7 cut(s) 312, 547, 585, 661, 1561, 2134, 2148
BspMI ACCTGC 1 cut(s) 2380
BspPI GGATC 4 cut(s) 812, 1315, 2141, 2154
BspT104I TTCGAA 3 cut(s) 921, 1398, 1425
BspT107I GGYRCC 1 cut(s) 659
BsrBI CCGCTC 1 cut(s) 657
BsrDI GCAATG 2 cut(s) 1918, 2052
BsrFI RCCGGY 1 cut(s) 2304
BsrGI TGTACA 2 cut(s) 557, 2453
BsrI ACTGG 3 cut(s) 245, 646, 2119
BssAI RCCGGY 1 cut(s) 2304
BssECI CCNNGG 7 cut(s) 157, 541, 635, 914, 1138, 1630, 2202
BssMI GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 1123
BssT1I CCWWGG 2 cut(s) 635, 1630
Bst2BI CACGAG 1 cut(s) 1123
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 8 cut(s) 92, 281, 664, 852, 872, 1080, 1318, 1501
Bst6I CTCTTC 4 cut(s) 38, 355, 1635, 1719
BstACI GRCGYC 1 cut(s) 75
BstAUI TGTACA 2 cut(s) 557, 2453
BstBAI YACGTR 2 cut(s) 906, 1052
BstBI TTCGAA 3 cut(s) 921, 1398, 1425
BstC8I GCNNGC 5 cut(s) 62, 901, 961, 1909, 2470
BstDEI CTNAG 7 cut(s) 168, 451, 731, 738, 1061, 1274, 1880
BstDSI CCRYGG 1 cut(s) 2202
BstF5I GGATG 4 cut(s) 1138, 1362, 1581, 2411
BstFNI CGCG 2 cut(s) 788, 1021
BstHHI GCGC 2 cut(s) 603, 1021
BstKTI GATC 7 cut(s) 6, 744, 799, 807, 1310, 1900, 2149
BstMAI GTCTC 4 cut(s) 96, 124, 231, 606
BstMBI GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
BstMWI GCNNNNNNNGC 3 cut(s) 490, 1253, 1292
BstNI CCWGG 1 cut(s) 915
BstNSI RCATGY 4 cut(s) 963, 990, 2174, 2454
BstSCI CCNGG 7 cut(s) 157, 497, 540, 541, 548, 913, 1137
BstSFI CTRYAG 1 cut(s) 2223
BstUI CGCG 2 cut(s) 788, 1021
BstV1I GCAGC 1 cut(s) 426
BstV2I GAAGAC 2 cut(s) 705, 1196
BstX2I RGATCY 3 cut(s) 1307, 1897, 2146
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 3 cut(s) 1307, 1897, 2146
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 5 cut(s) 291, 633, 736, 1907, 2308
BtgI CCRYGG 1 cut(s) 2202
BtsCI GGATG 4 cut(s) 1138, 1362, 1581, 2411
BtsI GCAGTG 2 cut(s) 458, 1506
BtsIMutI CAGTG 4 cut(s) 458, 857, 1085, 1506
BveI ACCTGC 1 cut(s) 2380
Cac8I GCNNGC 5 cut(s) 62, 901, 961, 1909, 2470
CciI TCATGA 2 cut(s) 471, 1864
CfoI GCGC 2 cut(s) 603, 1021
Cfr10I RCCGGY 1 cut(s) 2304
Cfr13I GGNCC 5 cut(s) 310, 330, 495, 632, 857
Cfr9I CCCGGG 1 cut(s) 541
CpoI CGGWCCG 1 cut(s) 495
CseI GACGC 3 cut(s) 83, 623, 1827
Csp6I GTAC 4 cut(s) 318, 558, 660, 2454
CspCI CAANNNNNGTGG 2 cut(s) 1989, 2024
CspI CGGWCCG 1 cut(s) 495
CviQI GTAC 4 cut(s) 318, 558, 660, 2454
DdeI CTNAG 7 cut(s) 168, 451, 731, 738, 1061, 1274, 1880
DpnI GATC 7 cut(s) 5, 743, 798, 806, 1309, 1899, 2148
DpnII GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
DrdI GACNNNNNNGTC 3 cut(s) 116, 245, 855
DseDI GACNNNNNNGTC 3 cut(s) 116, 245, 855
EaeI YGGCCR 1 cut(s) 2306
Eam1104I CTCTTC 4 cut(s) 38, 355, 1635, 1719
EarI CTCTTC 4 cut(s) 38, 355, 1635, 1719
Eco130I CCWWGG 2 cut(s) 635, 1630
Eco147I AGGCCT 2 cut(s) 291, 736
Eco32I GATATC 1 cut(s) 708
Eco47I GGWCC 4 cut(s) 310, 330, 495, 857
Eco57I CTGAAG 2 cut(s) 136, 2010
Eco72I CACGTG 2 cut(s) 906, 1052
Eco88I CYCGRG 1 cut(s) 541
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 913
EcoRV GATATC 1 cut(s) 708
EcoT14I CCWWGG 2 cut(s) 635, 1630
EcoT22I ATGCAT 1 cut(s) 965
ErhI CCWWGG 2 cut(s) 635, 1630
Esp3I CGTCTC 2 cut(s) 96, 606
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FaqI GGGAC 3 cut(s) 78, 659, 1187
FauNDI CATATG 2 cut(s) 562, 2020
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 1 cut(s) 1043
Fnu4HI GCNGC 1 cut(s) 415
FokI GGATG 4 cut(s) 1145, 1369, 1588, 2398
Fsp4HI GCNGC 1 cut(s) 415
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 2 cut(s) 602, 1020
GluI GCNGC 1 cut(s) 415
GsaI CCCAGC 1 cut(s) 1413
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 5 cut(s) 291, 633, 736, 1907, 2308
HapII CCGG 7 cut(s) 159, 498, 542, 549, 1034, 1138, 2305
HgaI GACGC 3 cut(s) 83, 623, 1827
HhaI GCGC 2 cut(s) 603, 1021
Hin1I GRCGYC 1 cut(s) 75
Hin6I GCGC 2 cut(s) 601, 1019
HinP1I GCGC 2 cut(s) 601, 1019
HincII GTYRAC 2 cut(s) 691, 1416
HindII GTYRAC 2 cut(s) 691, 1416
HindIII AAGCTT 2 cut(s) 374, 1538
HpaII CCGG 7 cut(s) 159, 498, 542, 549, 1034, 1138, 2305
HphI GGTGA 8 cut(s) 293, 345, 766, 924, 962, 2025, 2240, 2352
Hpy166II GTNNAC 6 cut(s) 691, 984, 1027, 1044, 1057, 1416
Hpy188I TCNGA 7 cut(s) 101, 454, 732, 741, 1474, 1665, 2074
Hpy8I GTNNAC 6 cut(s) 691, 984, 1027, 1044, 1057, 1416
Hpy99I CGWCG 1 cut(s) 113
HpyCH4III ACNGT 8 cut(s) 92, 281, 664, 852, 872, 1080, 1318, 1501
HpyCH4IV ACGT 6 cut(s) 111, 339, 782, 905, 1051, 1221
HpyF10VI GCNNNNNNNGC 3 cut(s) 490, 1253, 1292
HpyF3I CTNAG 7 cut(s) 168, 451, 731, 738, 1061, 1274, 1880
HpySE526I ACGT 6 cut(s) 111, 339, 782, 905, 1051, 1221
Hsp92I GRCGYC 1 cut(s) 75
HspAI GCGC 2 cut(s) 601, 1019
KpnI GGTACC 1 cut(s) 663
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
LmnI GCTCC 1 cut(s) 1565
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 2 cut(s) 1222, 1566
MaeII ACGT 6 cut(s) 111, 339, 782, 905, 1051, 1221
MaeIII GTNAC 7 cut(s) 340, 872, 1159, 1312, 1476, 2031, 2446
MalI GATC 7 cut(s) 5, 743, 798, 806, 1309, 1899, 2148
MbiI CCGCTC 1 cut(s) 657
MboI GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
MfeI CAATTG 1 cut(s) 2457
MflI RGATCY 3 cut(s) 1307, 1897, 2146
MhlI GDGCHC 1 cut(s) 1801
MlyI GAGTC 4 cut(s) 1031, 1236, 1763, 2404
MmeI TCCRAC 5 cut(s) 483, 646, 1702, 1790, 1993
Mph1103I ATGCAT 1 cut(s) 965
MroXI GAANNNNTTC 2 cut(s) 1193, 1659
MseI TTAA 7 cut(s) 1445, 1647, 1695, 1736, 1782, 1805, 2336
MslI CAYNNNNRTG 1 cut(s) 2278
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 7 cut(s) 159, 498, 542, 549, 1034, 1138, 2305
MspR9I CCNGG 7 cut(s) 159, 499, 542, 543, 550, 915, 1139
MunI CAATTG 1 cut(s) 2457
Mva1269I GAATGC 2 cut(s) 2131, 2262
MvaI CCWGG 1 cut(s) 915
MvnI CGCG 2 cut(s) 788, 1021
MwoI GCNNNNNNNGC 3 cut(s) 490, 1253, 1292
NciI CCSGG 6 cut(s) 159, 499, 542, 543, 550, 1139
NdeI CATATG 2 cut(s) 562, 2020
NdeII GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
NlaIV GGNNCC 7 cut(s) 312, 547, 585, 661, 1561, 2134, 2148
NmuCI GTSAC 4 cut(s) 872, 1159, 2031, 2446
NsbI TGCGCA 1 cut(s) 602
NsiI ATGCAT 1 cut(s) 965
NspI RCATGY 4 cut(s) 963, 990, 2174, 2454
NspV TTCGAA 3 cut(s) 921, 1398, 1425
OliI CACNNNNGTG 1 cut(s) 2278
PaeI GCATGC 1 cut(s) 963
PagI TCATGA 2 cut(s) 471, 1864
PceI AGGCCT 2 cut(s) 291, 736
PciI ACATGT 3 cut(s) 986, 2170, 2450
PcsI WCGNNNNNNNCGW 1 cut(s) 1227
PctI GAATGC 2 cut(s) 2131, 2262
PdmI GAANNNNTTC 2 cut(s) 1193, 1659
PfeI GAWTC 9 cut(s) 8, 147, 254, 764, 839, 923, 1871, 2216, 2231
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 1 cut(s) 416
PleI GAGTC 4 cut(s) 1031, 1235, 1763, 2403
PmaCI CACGTG 2 cut(s) 906, 1052
PmlI CACGTG 2 cut(s) 906, 1052
PpsI GAGTC 4 cut(s) 1031, 1235, 1763, 2403
Ppu21I YACGTR 2 cut(s) 906, 1052
PpuMI RGGWCCY 1 cut(s) 310
PscI ACATGT 3 cut(s) 986, 2170, 2450
PshBI ATTAAT 1 cut(s) 1445
PsiI TTATAA 2 cut(s) 1569, 2255
Psp1406I AACGTT 2 cut(s) 339, 782
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 913
PspCI CACGTG 2 cut(s) 906, 1052
PspFI CCCAGC 1 cut(s) 1409
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 7 cut(s) 312, 547, 585, 661, 1561, 2134, 2148
PspPI GGNCC 5 cut(s) 310, 330, 495, 632, 857
PspPPI RGGWCCY 1 cut(s) 310
PsuI RGATCY 3 cut(s) 1307, 1897, 2146
RsaI GTAC 4 cut(s) 319, 559, 661, 2455
RsaNI GTAC 4 cut(s) 318, 558, 660, 2454
RseI CAYNNNNRTG 1 cut(s) 2278
Rsr2I CGGWCCG 1 cut(s) 495
RsrII CGGWCCG 1 cut(s) 495
SaqAI TTAA 7 cut(s) 1445, 1647, 1695, 1736, 1782, 1805, 2336
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 7 cut(s) 3, 741, 796, 804, 1307, 1897, 2146
Sau96I GGNCC 5 cut(s) 310, 330, 495, 632, 857
SchI GAGTC 4 cut(s) 1031, 1236, 1763, 2404
ScrFI CCNGG 7 cut(s) 159, 499, 542, 543, 550, 915, 1139
SduI GDGCHC 1 cut(s) 1801
SfaNI GCATC 2 cut(s) 1222, 1566
SfcI CTRYAG 1 cut(s) 2223
SfuI TTCGAA 3 cut(s) 921, 1398, 1425
SinI GGWCC 4 cut(s) 310, 330, 495, 857
SmaI CCCGGG 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 2278
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 1436
SphI GCATGC 1 cut(s) 963
SseBI AGGCCT 2 cut(s) 291, 736
SsiI CCGC 8 cut(s) 175, 333, 493, 587, 657, 855, 974, 1130
SspI AATATT 1 cut(s) 1971
StuI AGGCCT 2 cut(s) 291, 736
StyD4I CCNGG 7 cut(s) 157, 497, 540, 541, 548, 913, 1137
StyI CCWWGG 2 cut(s) 635, 1630
TaaI ACNGT 8 cut(s) 92, 281, 664, 852, 872, 1080, 1318, 1501
TaiI ACGT 6 cut(s) 114, 342, 785, 908, 1054, 1224
TaqI TCGA 7 cut(s) 6, 921, 1225, 1230, 1398, 1425, 1824
TatI WGTACW 2 cut(s) 557, 2453
TfiI GAWTC 9 cut(s) 8, 147, 254, 764, 839, 923, 1871, 2216, 2231
Tru1I TTAA 7 cut(s) 1445, 1647, 1695, 1736, 1782, 1805, 2336
Tru9I TTAA 7 cut(s) 1445, 1647, 1695, 1736, 1782, 1805, 2336
TscAI CASTG 4 cut(s) 465, 857, 1085, 1513
TseFI GTSAC 4 cut(s) 872, 1159, 2031, 2446
TseI GCWGC 1 cut(s) 414
Tsp45I GTSAC 4 cut(s) 872, 1159, 2031, 2446
TspGWI ACGGA 3 cut(s) 276, 861, 1552
TspMI CCCGGG 1 cut(s) 541
TspRI CASTG 4 cut(s) 465, 857, 1085, 1513
Van91I CCANNNNNTGG 1 cut(s) 222
VpaK11BI GGWCC 4 cut(s) 310, 330, 495, 857
VspI ATTAAT 1 cut(s) 1445
XapI RAATTY 3 cut(s) 163, 2158, 2291
XceI RCATGY 4 cut(s) 963, 990, 2174, 2454
XmaI CCCGGG 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 1043
XmnI GAANNNNTTC 2 cut(s) 1193, 1659
Zsp2I ATGCAT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.