FvH4_3g03301

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000099
Physical Location & Seq
Reverse (-)
1775603 .. 1779237
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03301.t1

Sequence Viewer

Length: 2466 bp
ATGCATTTTGCAAAAGACTTTCTGAATACATTGATTCAGCTTCTTCTTCTTCTTCGGCTGTGCAAATCTTTGGAAACAACTATCATTTCAAGCAAATCCGTCAAAGATGGTGACTTCCTTGTCTCTGAAAACCAGACGTTTGTGCTTGGATTCTTCAGTCCCGGCACATCAAGCAACCGCTATGTTGGTATTTGGTATAAGTTCTCAAAAGAAATGGTTGTGTGGGTAGCTAACAGAGACAATCCAGTCAATGATACCTCAGGTATCCTCACAATTAGTACTGATGGAAATCTTGTACTACTTCATAACAGCACTCAAGGCCTTCCTCTATGGTCAACGAATGTTTCTGTCACACCAGCAAGTAGAAATAGTAATATGGTGCAGCTCCTGGATTCAGGAAATCTTGTTTTGGTTAATGAGCTAGTCAACAACAAGAGTGGAGTCTTATGGCAAAGCTTTGATCATCCTACACATGTTCTTCTTGCAAACATGAAGATTGGGCTGGACCATGGTAAGAACATATTCCTCACATCTTGGAACTCGAAAAATGACCCTGGAACTGGGATTTGCTCTTTAAGGACAGAACCAAATGGGTCCCCTCAGATATTCTTGTATAAGAATAGGACTAAGCTGTGGCGGGCAGGGCCATGGAATGGGCTTCACTGGAGTGGATTACCTAATATGAATAGGCAAAGCAACACTAATGAGTTTGACATAGTGTTTGTGAACAACAAAGATGAGGTTACTACAAAGTGGGTTGTTCTTGACCCATCAGTTTTCTCAGTTATAGTAATTGATGGATCTGGATCACTTCAACAGCTAGCATGGCAGGGACAGAAAGGGTGGGTCACAATTGTGTCCACACCAACGGATGTTTGTGACAAGTATGGAACGTGTGGTCCGTTTGGTGTTTGTAATGCATACACGGTTAACTGGGAGAAATGTGTGTGCTTTCCTGGATATGAACCCAGCTCCCCAGAGAATTGGAAATTAAGAGAATGGTCTAGTGGGTGCAAGAGGCAGCAGGGAGCGCCATCCATGTGTAGGAATGGAGAGGGCTTTGTAAAGATACAAAATGTGAAGGTTCCAGATATTTCCACAATCAAATTGGATATCAATTGGACTTTGGAAGCATGTGAGCAGAAGTGTTTGAATAACTGCTCATGCTTGGCGTATGCCAGTGCAGATGTGAGAAATGGGGGCAGCGGATGCATTACATGGTATGGAGATTTAATGGATACTACTCAGTTTGCTCAAGGACAAGGACAAGATGTGTACATTCGCGCTGATGCTATAGTGTCAGCCAAGTACACAAAGAAGTCAAAGAGGTTCCTTGCCAAAAAGGGGATGTGGTTGATTTTGGTATTCCCACCCATTTCTATGGTATTCTTGATCCTTTATGGAGTTTGGTGCTTAAGGAGGAAGACAAAAGGCTCTCTAACGAAGCAAGATGGAAGTAGAACTCACAAAGACTTGGAATATTTTGATTTAAAGAGCATACTTGTTGCCACTGACAATTTCTCCACTGCTAACAAGCTAGGGGAAGGAGGATTTGGCCCAGTTTATAAGGGCCTACTAGCTAATGGAGAGGAGATTGCTGTGAAAAGACTATCGAAGAACTCCGGACAAGGTTTAGAACAGTTTAAAACTGAGCTTAAGCTGATAGCGAAACTTCAGCACAGGAACCTGGTTAGGCTTCTTGGTTGTTGCATCAATACAGAAGAGAAGATGTTGATTTATGAATACTTGCCAAACAAGAGCTTAGACTTTTTCATATTTGATAGAAGTAGAAGCTTGTCTATAGACTGGAAAGACCGTTTTGAAATTATAAATGGAATTGCTCGTGGGGTCCTATATCTCCATCAAGACTCCAGATTAAAGATTGTCCACAGGGACATAAAAGCTAGCAATGTCTTACTGGATTCAACAATGAATCCAAAGATCTCAGATTTTGGACTTGCTAAAATGCTTGGGGAAGACCAGCTCCAAGCAAATACAAACAGAGTGATTGGAACGTATGGCTACATGTCACCTGAATATGCAATGGAAGGGCGATATTCAACAAAATCTGATGTTTTTAGCTTTGGTGTCTTACTGCTAGAGATCATCAGTGGCAAGAGGAACACTGACCACAATCTTGACAGCCCCTCCCGCAATTTGATTGGAAAAATCTGGGATATGTGGAGAGAGGGACAAGCATTGGGAATAGTTGATCCATCTTTGAGTTTGTATCCTGCTCATGAGGTTTCAAGATGCATCCACATTGGGCTACTATGCATGCAAGAGTCTACAACAGATCGGCCAACAATGTCGGAAGTAGTTTACATGCTGGGCAAAGAAACACCTCTCACTTCTCCTAAGAAACCTGCATTCATATTACAATCCAGCAATCCAAACTCAGAAGAAGCACCAGGAAGAGGACTTTCTCTAAATAATGTAACAATAACCATGCTTGATGCCCGCTAA

Protein Analysis

822

Amino Acids

91.82

Weight (kDa)

8.7

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 179 3.4e-30 D-mannose binding lectin
S_locus_glycop PF00954 212 - 324 8.8e-25 S-locus glycoprotein domain
PAN_2 PF08276 348 - 414 4.6e-21 PAN-like domain
Pkinase PF00069 508 - 774 1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 509 - 775 1.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1566, 1829
AasI GACNNNNNNGTC 2 cut(s) 119, 245
Acc36I ACCTGC 1 cut(s) 2374
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 2288
AccII CGCG 1 cut(s) 1284
AccIII TCCGGA 1 cut(s) 1622
AciI CCGC 5 cut(s) 178, 637, 1206, 2152, 2461
AclWI GGATC 4 cut(s) 808, 814, 1387, 2207
AcoI YGGCCR 1 cut(s) 2300
AcuI CTGAAG 2 cut(s) 139, 1658
AfaI GTAC 4 cut(s) 280, 297, 1277, 1310
AfiI CCNNNNNNNGG 5 cut(s) 560, 653, 1044, 1344, 2417
AflII CTTAAG 2 cut(s) 1414, 1655
AflIII ACRYGT 3 cut(s) 472, 893, 2025
AgsI TTSAA 7 cut(s) 90, 815, 1153, 1823, 1926, 2061, 2250
AjnI CCWGG 5 cut(s) 387, 553, 955, 1686, 2410
AjuI GAANNNNNNNTTGG 2 cut(s) 1536, 1568
AleI CACNNNNGTG 2 cut(s) 666, 854
AloI GAACNNNNNNTCC 2 cut(s) 883, 915
Alw26I GTCTC 2 cut(s) 127, 231
AlwI GGATC 4 cut(s) 808, 814, 1387, 2207
AlwNI CAGNNNCTG 1 cut(s) 388
Aor13HI TCCGGA 1 cut(s) 1622
AoxI GGCC 5 cut(s) 319, 644, 1555, 1570, 2300
ApeKI GCWGC 3 cut(s) 382, 1021, 1203
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 2 cut(s) 1033, 1286
AspS9I GGNCC 7 cut(s) 505, 594, 644, 899, 1556, 1570, 1849
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 122, 2022
AsuNHI GCTAGC 2 cut(s) 820, 1904
AvaII GGWCC 4 cut(s) 505, 594, 899, 1849
AxyI CCTNAGG 1 cut(s) 259
BauI CACGAG 1 cut(s) 1842
BbsI GAAGAC 2 cut(s) 1430, 1983
BbvI GCAGC 3 cut(s) 394, 1033, 1215
BccI CCATC 8 cut(s) 101, 278, 778, 791, 1042, 1445, 1869, 2224
BciT130I CCWGG 5 cut(s) 389, 555, 957, 1688, 2412
BciVI GTATCC 3 cut(s) 275, 1231, 2241
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 2 cut(s) 127, 231
BfaI CTAG 7 cut(s) 422, 821, 1005, 1538, 1577, 1905, 2099
BfmI CTRYAG 2 cut(s) 1293, 1800
BfoI RGCGCY 1 cut(s) 1034
BfrI CTTAAG 2 cut(s) 1414, 1655
BfuAI ACCTGC 1 cut(s) 2374
BfuI GTATCC 3 cut(s) 275, 1231, 2241
BglII AGATCT 1 cut(s) 1941
BisI GCNGC 3 cut(s) 383, 1022, 1204
BlsI GCNGC 3 cut(s) 384, 1023, 1205
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 6 cut(s) 162, 389, 555, 957, 1688, 2412
Bme18I GGWCC 4 cut(s) 505, 594, 899, 1849
BmgT120I GGNCC 7 cut(s) 505, 594, 644, 899, 1556, 1570, 1849
BmiI GGNNCC 6 cut(s) 595, 596, 1086, 1331, 1685, 1850
BmrFI CCNGG 6 cut(s) 162, 389, 555, 957, 1688, 2412
BmrI ACTGGG 3 cut(s) 570, 943, 1553
BmsI GCATC 6 cut(s) 1199, 1279, 1719, 2243, 2265, 2446
BmtI GCTAGC 2 cut(s) 824, 1908
BmuI ACTGGG 3 cut(s) 570, 943, 1553
BpiI GAAGAC 2 cut(s) 1430, 1983
BpmI CTGGAG 2 cut(s) 685, 1855
BpuEI CTTGAG 2 cut(s) 300, 1239
BpuMI CCSGG 1 cut(s) 162
BsaBI GATNNNNATC 2 cut(s) 288, 805
BsaJI CCNNGG 3 cut(s) 508, 553, 647
BsaWI WCCGGW 1 cut(s) 1622
BsaXI ACNNNNNCTCC 4 cut(s) 1505, 1535, 2132, 2162
Bsc4I CCNNNNNNNGG 5 cut(s) 560, 653, 1044, 1344, 2417
Bse1I ACTGG 8 cut(s) 245, 565, 668, 938, 1179, 1559, 1811, 1923
Bse21I CCTNAGG 1 cut(s) 259
Bse3DI GCAATG 2 cut(s) 1915, 2049
Bse8I GATNNNNATC 2 cut(s) 288, 805
BseAI TCCGGA 1 cut(s) 1622
BseBI CCWGG 5 cut(s) 389, 555, 957, 1688, 2412
BseDI CCNNGG 3 cut(s) 508, 553, 647
BseGI GGATG 6 cut(s) 463, 877, 1034, 1214, 1353, 2256
BseJI GATNNNNATC 2 cut(s) 288, 805
BseLI CCNNNNNNNGG 5 cut(s) 560, 653, 1044, 1344, 2417
BseMI GCAATG 2 cut(s) 1915, 2049
BseMII CTCAG 7 cut(s) 273, 614, 795, 1259, 1641, 1959, 2412
BseNI ACTGG 8 cut(s) 245, 565, 668, 938, 1179, 1559, 1811, 1923
BseRI GAGGAG 1 cut(s) 1604
BseXI GCAGC 3 cut(s) 394, 1033, 1215
BseYI CCCAGC 2 cut(s) 968, 2329
BsgI GTGCAG 2 cut(s) 401, 1203
Bsh1236I CGCG 1 cut(s) 1284
BshFI GGCC 5 cut(s) 321, 646, 1557, 1572, 2302
BsiSI CCGG 2 cut(s) 162, 1623
BslFI GGGAC 5 cut(s) 144, 580, 846, 1907, 2205
BslI CCNNNNNNNGG 5 cut(s) 560, 653, 1044, 1344, 2417
BsmAI GTCTC 2 cut(s) 127, 231
BsmFI GGGAC 5 cut(s) 144, 580, 846, 1907, 2205
BsmI GAATGC 1 cut(s) 2369
BsnI GGCC 5 cut(s) 321, 646, 1557, 1572, 2302
Bsp13I TCCGGA 1 cut(s) 1622
Bsp1407I TGTACA 1 cut(s) 1275
Bsp143I GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
Bsp19I CCATGG 2 cut(s) 508, 647
BspACI CCGC 5 cut(s) 178, 637, 1206, 2152, 2461
BspANI GGCC 5 cut(s) 321, 646, 1557, 1572, 2302
BspCNI CTCAG 7 cut(s) 272, 613, 794, 1258, 1642, 1958, 2411
BspEI TCCGGA 1 cut(s) 1622
BspFNI CGCG 1 cut(s) 1284
BspHI TCATGA 1 cut(s) 2239
BspLI GGNNCC 6 cut(s) 595, 596, 1086, 1331, 1685, 1850
BspMI ACCTGC 1 cut(s) 2374
BspOI GCTAGC 2 cut(s) 824, 1908
BspPI GGATC 4 cut(s) 808, 814, 1387, 2207
BspTI CTTAAG 2 cut(s) 1414, 1655
BsrDI GCAATG 2 cut(s) 1915, 2049
BsrGI TGTACA 1 cut(s) 1275
BsrI ACTGG 8 cut(s) 245, 565, 668, 938, 1179, 1559, 1811, 1923
BssECI CCNNGG 3 cut(s) 508, 553, 647
BssMI GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
BssSI CACGAG 1 cut(s) 1842
BssT1I CCWWGG 2 cut(s) 508, 647
Bst2BI CACGAG 1 cut(s) 1842
Bst2UI CCWGG 5 cut(s) 389, 555, 957, 1688, 2412
Bst4CI ACNGT 3 cut(s) 928, 1641, 1817
Bst6I CTCTTC 2 cut(s) 1716, 2410
BstAFI CTTAAG 2 cut(s) 1414, 1655
BstAPI GCANNNNNTGC 1 cut(s) 1209
BstAUI TGTACA 1 cut(s) 1275
BstC8I GCNNGC 5 cut(s) 639, 822, 1906, 2279, 2461
BstDSI CCRYGG 2 cut(s) 508, 647
BstF5I GGATG 6 cut(s) 463, 877, 1034, 1214, 1353, 2256
BstFNI CGCG 1 cut(s) 1284
BstH2I RGCGCY 1 cut(s) 1034
BstHHI GCGC 2 cut(s) 1033, 1286
BstKTI GATC 8 cut(s) 463, 803, 809, 1395, 1944, 2106, 2215, 2299
BstMAI GTCTC 2 cut(s) 127, 231
BstMBI GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
BstMWI GCNNNNNNNGC 7 cut(s) 171, 318, 643, 826, 1030, 1209, 2151
BstNI CCWGG 5 cut(s) 389, 555, 957, 1688, 2412
BstNSI RCATGY 5 cut(s) 476, 1137, 2029, 2281, 2329
BstSCI CCNGG 6 cut(s) 160, 387, 553, 955, 1686, 2410
BstSFI CTRYAG 2 cut(s) 1293, 1800
BstUI CGCG 1 cut(s) 1284
BstV1I GCAGC 3 cut(s) 394, 1033, 1215
BstV2I GAAGAC 2 cut(s) 1430, 1983
BstX2I RGATCY 2 cut(s) 800, 1941
BstXI CCANNNNNNTGG 2 cut(s) 984, 1381
BstYI RGATCY 2 cut(s) 800, 1941
Bsu36I CCTNAGG 1 cut(s) 259
BsuI GTATCC 3 cut(s) 275, 1231, 2241
BsuRI GGCC 5 cut(s) 321, 646, 1557, 1572, 2302
BtgI CCRYGG 2 cut(s) 508, 647
BtsCI GGATG 6 cut(s) 463, 877, 1034, 1214, 1353, 2256
BtsI GCAGTG 1 cut(s) 1524
BtsIMutI CAGTG 6 cut(s) 661, 1186, 1509, 1524, 2116, 2124
BveI ACCTGC 1 cut(s) 2374
Cac8I GCNNGC 5 cut(s) 639, 822, 1906, 2279, 2461
CaiI CAGNNNCTG 1 cut(s) 388
CciI TCATGA 1 cut(s) 2239
CfoI GCGC 2 cut(s) 1033, 1286
Cfr13I GGNCC 7 cut(s) 505, 594, 644, 899, 1556, 1570, 1849
CsiI ACCWGGT 1 cut(s) 1686
Csp6I GTAC 4 cut(s) 279, 296, 1276, 1309
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviQI GTAC 4 cut(s) 279, 296, 1276, 1309
DpnI GATC 8 cut(s) 462, 802, 808, 1394, 1943, 2105, 2214, 2298
DpnII GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
DraI TTTAAA 2 cut(s) 1491, 1645
DrdI GACNNNNNNGTC 2 cut(s) 119, 245
DseDI GACNNNNNNGTC 2 cut(s) 119, 245
EaeI YGGCCR 1 cut(s) 2300
Eam1104I CTCTTC 2 cut(s) 1716, 2410
EarI CTCTTC 2 cut(s) 1716, 2410
Eco130I CCWWGG 2 cut(s) 508, 647
Eco147I AGGCCT 1 cut(s) 321
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 4 cut(s) 505, 594, 899, 1849
Eco57I CTGAAG 2 cut(s) 139, 1658
Eco81I CCTNAGG 1 cut(s) 259
EcoO109I RGGNCCY 3 cut(s) 594, 1570, 1849
EcoRII CCWGG 5 cut(s) 387, 553, 955, 1686, 2410
EcoRV GATATC 1 cut(s) 1114
EcoT14I CCWWGG 2 cut(s) 508, 647
EcoT22I ATGCAT 5 cut(s) 6, 922, 1214, 2258, 2279
ErhI CCWWGG 2 cut(s) 508, 647
FaqI GGGAC 5 cut(s) 144, 580, 846, 1907, 2205
FauI CCCGC 2 cut(s) 630, 2159
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 2288
Fnu4HI GCNGC 3 cut(s) 383, 1022, 1204
FokI GGATG 6 cut(s) 450, 884, 1021, 1221, 1360, 2243
Fsp4HI GCNGC 3 cut(s) 383, 1022, 1204
FspBI CTAG 7 cut(s) 422, 821, 1005, 1538, 1577, 1905, 2099
GlaI GCGC 2 cut(s) 1032, 1285
GluI GCNGC 3 cut(s) 383, 1022, 1204
GsaI CCCAGC 2 cut(s) 972, 2333
GsuI CTGGAG 2 cut(s) 685, 1855
HaeII RGCGCY 1 cut(s) 1034
HaeIII GGCC 5 cut(s) 321, 646, 1557, 1572, 2302
HapII CCGG 2 cut(s) 162, 1623
HhaI GCGC 2 cut(s) 1033, 1286
Hin6I GCGC 2 cut(s) 1031, 1284
HinP1I GCGC 2 cut(s) 1031, 1284
HincII GTYRAC 3 cut(s) 336, 427, 931
HindII GTYRAC 3 cut(s) 336, 427, 931
HindIII AAGCTT 2 cut(s) 454, 1792
HinfI GANTC 8 cut(s) 34, 150, 392, 441, 1868, 1922, 1933, 2285
HpaI GTTAAC 1 cut(s) 931
HpaII CCGG 2 cut(s) 162, 1623
HphI GGTGA 2 cut(s) 122, 2022
Hpy188I TCNGA 7 cut(s) 24, 127, 603, 1948, 2071, 2314, 2401
HpyAV CCTTC 4 cut(s) 332, 1075, 1538, 2042
HpyCH4III ACNGT 3 cut(s) 928, 1641, 1817
HpyCH4IV ACGT 3 cut(s) 137, 893, 2015
HpyF10VI GCNNNNNNNGC 7 cut(s) 171, 318, 643, 826, 1030, 1209, 2151
HpySE526I ACGT 3 cut(s) 137, 893, 2015
HspAI GCGC 2 cut(s) 1031, 1284
KflI GGGWCCC 1 cut(s) 594
Kpn2I TCCGGA 1 cut(s) 1622
Ksp22I TGATCA 1 cut(s) 460
KspAI GTTAAC 1 cut(s) 931
Kzo9I GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
LmnI GCTCC 4 cut(s) 390, 977, 1028, 1989
Lsp1109I GCAGC 3 cut(s) 394, 1033, 1215
LweI GCATC 6 cut(s) 1199, 1279, 1719, 2243, 2265, 2446
MabI ACCWGGT 1 cut(s) 1686
MaeI CTAG 7 cut(s) 422, 821, 1005, 1538, 1577, 1905, 2099
MaeII ACGT 3 cut(s) 137, 893, 2015
MaeIII GTNAC 7 cut(s) 110, 349, 742, 847, 878, 2028, 2437
MalI GATC 8 cut(s) 462, 802, 808, 1394, 1943, 2105, 2214, 2298
MboI GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
MfeI CAATTG 2 cut(s) 852, 1117
MflI RGATCY 2 cut(s) 800, 1941
MlyI GAGTC 3 cut(s) 450, 1862, 2294
MmeI TCCRAC 1 cut(s) 2292
Mph1103I ATGCAT 5 cut(s) 6, 922, 1214, 2258, 2279
MroI TCCGGA 1 cut(s) 1622
MroXI GAANNNNTTC 1 cut(s) 521
MslI CAYNNNNRTG 4 cut(s) 666, 854, 1039, 1379
MspA1I CMGCKG 1 cut(s) 1206
MspCI CTTAAG 2 cut(s) 1414, 1655
MspI CCGG 2 cut(s) 162, 1623
MspR9I CCNGG 6 cut(s) 162, 389, 555, 957, 1688, 2412
MunI CAATTG 2 cut(s) 852, 1117
Mva1269I GAATGC 1 cut(s) 2369
MvaI CCWGG 5 cut(s) 389, 555, 957, 1688, 2412
MvnI CGCG 1 cut(s) 1284
MwoI GCNNNNNNNGC 7 cut(s) 171, 318, 643, 826, 1030, 1209, 2151
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 2 cut(s) 508, 647
NdeII GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
NheI GCTAGC 2 cut(s) 820, 1904
NlaIV GGNNCC 6 cut(s) 595, 596, 1086, 1331, 1685, 1850
NmuCI GTSAC 5 cut(s) 110, 349, 847, 878, 2028
NsiI ATGCAT 5 cut(s) 6, 922, 1214, 2258, 2279
NspI RCATGY 5 cut(s) 476, 1137, 2029, 2281, 2329
OliI CACNNNNGTG 2 cut(s) 666, 854
PaeI GCATGC 1 cut(s) 2281
PagI TCATGA 1 cut(s) 2239
PceI AGGCCT 1 cut(s) 321
PciI ACATGT 2 cut(s) 472, 2025
PcsI WCGNNNNNNNCGW 1 cut(s) 899
PctI GAATGC 1 cut(s) 2369
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 5 cut(s) 34, 150, 392, 1922, 1933
PflMI CCANNNNNTGG 1 cut(s) 653
PfoI TCCNGGA 2 cut(s) 387, 955
PkrI GCNGC 3 cut(s) 384, 1023, 1205
PleI GAGTC 3 cut(s) 449, 1862, 2293
PpsI GAGTC 3 cut(s) 449, 1862, 2293
PpuMI RGGWCCY 2 cut(s) 594, 1849
PscI ACATGT 2 cut(s) 472, 2025
PsiI TTATAA 2 cut(s) 1566, 1829
Psp5II RGGWCCY 2 cut(s) 594, 1849
Psp6I CCWGG 5 cut(s) 387, 553, 955, 1686, 2410
PspFI CCCAGC 2 cut(s) 968, 2329
PspGI CCWGG 5 cut(s) 387, 553, 955, 1686, 2410
PspN4I GGNNCC 6 cut(s) 595, 596, 1086, 1331, 1685, 1850
PspPI GGNCC 7 cut(s) 505, 594, 644, 899, 1556, 1570, 1849
PspPPI RGGWCCY 2 cut(s) 594, 1849
PstNI CAGNNNCTG 1 cut(s) 388
PsuI RGATCY 2 cut(s) 800, 1941
RsaI GTAC 4 cut(s) 280, 297, 1277, 1310
RsaNI GTAC 4 cut(s) 279, 296, 1276, 1309
RseI CAYNNNNRTG 4 cut(s) 666, 854, 1039, 1379
SatI GCNGC 3 cut(s) 383, 1022, 1204
Sau3AI GATC 8 cut(s) 460, 800, 806, 1392, 1941, 2103, 2212, 2296
Sau96I GGNCC 7 cut(s) 505, 594, 644, 899, 1556, 1570, 1849
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 3 cut(s) 450, 1862, 2294
ScrFI CCNGG 6 cut(s) 162, 389, 555, 957, 1688, 2412
SexAI ACCWGGT 1 cut(s) 1686
SfaNI GCATC 6 cut(s) 1199, 1279, 1719, 2243, 2265, 2446
SfcI CTRYAG 2 cut(s) 1293, 1800
SinI GGWCC 4 cut(s) 505, 594, 899, 1849
SmiMI CAYNNNNRTG 4 cut(s) 666, 854, 1039, 1379
SmlI CTYRAG 4 cut(s) 315, 1254, 1414, 1655
SmoI CTYRAG 4 cut(s) 315, 1254, 1414, 1655
SphI GCATGC 1 cut(s) 2281
SseBI AGGCCT 1 cut(s) 321
SsiI CCGC 5 cut(s) 178, 637, 1206, 2152, 2461
SspI AATATT 1 cut(s) 1481
SspMI CTAG 7 cut(s) 422, 821, 1005, 1538, 1577, 1905, 2099
StuI AGGCCT 1 cut(s) 321
StyD4I CCNGG 6 cut(s) 160, 387, 553, 955, 1686, 2410
StyI CCWWGG 2 cut(s) 508, 647
TaaI ACNGT 3 cut(s) 928, 1641, 1817
TaiI ACGT 3 cut(s) 140, 896, 2018
TaqI TCGA 2 cut(s) 542, 1613
TatI WGTACW 4 cut(s) 278, 295, 1275, 1308
TfiI GAWTC 5 cut(s) 34, 150, 392, 1922, 1933
TscAI CASTG 6 cut(s) 668, 1186, 1516, 1531, 2116, 2131
TseFI GTSAC 5 cut(s) 110, 349, 847, 878, 2028
TseI GCWGC 3 cut(s) 382, 1021, 1203
Tsp45I GTSAC 5 cut(s) 110, 349, 847, 878, 2028
TspDTI ATGAA 8 cut(s) 293, 506, 698, 978, 1755, 1762, 1946, 2362
TspGWI ACGGA 3 cut(s) 88, 884, 891
TspRI CASTG 6 cut(s) 668, 1186, 1516, 1531, 2116, 2131
Van91I CCANNNNNTGG 1 cut(s) 653
Vha464I CTTAAG 2 cut(s) 1414, 1655
VpaK11BI GGWCC 4 cut(s) 505, 594, 899, 1849
XceI RCATGY 5 cut(s) 476, 1137, 2029, 2281, 2329
XcmI CCANNNNNNNNNTGG 1 cut(s) 1105
XmiI GTMKAC 1 cut(s) 2288
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 7 cut(s) 422, 821, 1005, 1538, 1577, 1905, 2099
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 5 cut(s) 6, 922, 1214, 2258, 2279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.