MD05G1333700.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000099
Physical Location & Seq
Forward (+)
45677251 .. 45682850
5600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1333700.v1.1.491

Sequence Viewer

Length: 2559 bp
ATGAGAAAGCTAACAATGAATTCTACTGAATACTTTTTTGTCAGTACTGTATTGCTCCTCTTCCTTGTGATTCTTCCCTCTTCCATTTCGGTTACCCTAGACGCCATTACACCAAACCAACCCCTAAGAGAGGACGACGTTCTTCTCTCCACCACTAAATTCTTTGCACTAGGGTTTTTTAAGCCAGGCAATTCTCGTAACCGTTACATCGGAGTTTGGTACAACAAAATTCCAATCCAAACCATTGTTTGGATTGCAAACAGAGAAAACCCCATAGTTCCTACTGCTGGAACTGGACTTCTAGCCATTCATGCAGATCACGGTGGCCTTGTTTTGTATGGGGAGGACCAAAATACCCCTATCTGGTCCACTAATCTCACCGTCTCTTCTCCAAACAATTCCGTGATAGCCAAGCTTTTGGATACGGGAAATCTTGTTGTAGTTGAAATTAATGGCGGTAGTAGTGAAAAGGTGTTGTGGCAAGGTTTTGATTATCCCACAAATACGATGCTTCCTGTTATGAAACTTGGGCTGGACAGGCGATCCGGGTTCAACTGGTTCCTCACATCTTGGAAATCCCAGGATGACCCGGGAATGGGCAACTGTTCGTACCGGATAGAGCCAAGTGAGAGTCCACAGGGGTTCTTATACATGGGTCAGACTCCATTGTGGCGGACTGGACCTTGGATCGGTGAAAGATGGTCCGGGGTGCCTCAAATGCAAAATAAGATCTTCTTCAACGTTACTTTTAAGAACAATCAAGATGAGGTATCCATCATGTATAGTGTTGTTTATGAATCAATCTTCGTAAAGATGGTGATCGATGAATCAGGAAACATTGAACGGTCCGCATGGCAAGATAATAAATGGGTCCAGTATTGGTCCGCGCCGGATGGGCAGTGTGATTTTTACGGGAAGTGTGGTCCGAATAGCATTTGTGACCCATACCCAAAATTTGCCGATAAATTTGAGTGCAAATGCCTACCTGGATTCGAACCCAAGTTGCGACATGAGTGGTATTTGAGAGATTGGTCAGGCGGGTGCGTGAGGCAAAAAGGATCATCCGTTTGCCAAAACGGGGAAGGGTTCGTGAAGTTGGAACGTGTGAAGGTGCCGGACACTTCTACGGCACGTGTGAACATGAGTAGGAGTCGGGAAGCATGTAAAGAAGAATGCCTGAGAAATTGTTCTTGCACGGCATACGCAAATGCAGATGAAAGGCAGGGAGGGAGTGGCTGTATAACATGGCATGGGGACTTGATGGACACAAGGACTTATTTGGATACGGGTCAAGATTTATATGTGCGAGTTCATGCAAATGTTTCAGCTCAAAATGCAAAGAAGTCAAACAGTTCTTTTGGCAAGAAGAGGAAGCTGGAAGTTTCAGTAGCATGTGGTCTATTGTTCTTCCTCTTGTTTTCCCTTGCATGTTGGTTGGTAAAGAGGAAGAGAAAAGGTCAGAGAAGTCAGGATAAATTTTTCAATGTGACCGTAGCATCAACCGCCTGGGAAGATTCTTCTGCTAGAACAAATATTGATGAAAGTGGAATAAACTCTGAATTACCATTCTTTGAACTAAGTACCATAGTTAAGGCCACAAATAATTTCGCTTCCAACAACAAGCTTGGAACAGGTGGTTTTGGCTCCGTTTATAAGGGTGTGCTTGATAATGGAAAGGACATAGCAGTGAAAAGACTAGCCAAGAATTCTGGCCAAGGAATTGGAGAGTTTAAGAATGAAGTTGTGCTGCTTTCAAAGCTCCAACACAGGAACCTTGTAAGGATCATGGGTTGCTGCGTTCAAGATGAAGAGAAGATGCTGATCTATGAATACTTGCCAAACAAAAGTCTTGACTTTTTCATTTTCGCAAAAGAGGCGTTCTTGGATTGGACAAGATGCTTTGAGATAATCTGTGGGATTGCTAGAGGGATCTTATATCTTCATCAGGATTCGAGATTAAGAATCATCCATAAAGATCTAAAGGCCAGCAATGTTCTGTTGGATTCTGCTATGAACCCCAAGATTTCAGATTTTGGTATGGCTAGGATATTTGGAGCTGAACAAATTGAAGCAAATACAAACCGTGTGGTTGGGACATATGGTTATATGTCACCAGAGTACGCAATGGAAGGATTTTTTTCAGTAAAGTCTGACGTATATAGCTTCGGCATTTTACTACTAGAAATTGTTACTGGCAGAAAGAACACCGGCTACTACCAAGATAGTCCTTACTCAAATATAATTGGACATGTTTGGGACTTGTGGAAGGAAAATAAAGCCTTGGAAATCATTGATTCATCTCTAGGAGAATCATACCCTGTCAATGAAGTTCTAAGATGTATTCACATTGCCCTCTTGTGCGTGCAAGAGCAAGCGAAGGATCGTCCACTCATGTCAGCAGTGGTTTCCATGTTGGGTAATGATGCAGCAATTCCTTCACCAAAGCAACCTGGATTTTTGTTGAAGAGAGGTTATCATACTAGTGGAGACCCATCAACCAATACTGATGGAGCTTGCTCTGTAAATGACATGACCTATACAGAAGCAGAAGGTCGCTAA

Protein Analysis

853

Amino Acids

95.61

Weight (kDa)

7.41

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 81 - 192 7.9e-31 D-mannose binding lectin
S_locus_glycop PF00954 224 - 334 6.1e-27 S-locus glycoprotein domain
PAN_2 PF08276 357 - 423 7.1e-23 PAN-like domain
Pkinase PF00069 537 - 737 4.3e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 539 - 805 6.2e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 807 - 852 2.1e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1653
AccB1I GGYRCC 2 cut(s) 709, 1111
AccB7I CCANNNNNTGG 1 cut(s) 249
AccII CGCG 1 cut(s) 887
AciI CCGC 6 cut(s) 456, 673, 849, 885, 1038, 1503
AclI AACGTT 1 cut(s) 741
AclWI GGATC 6 cut(s) 537, 695, 1066, 1790, 1937, 2388
AcoI YGGCCR 1 cut(s) 1711
AcsI RAATTY 7 cut(s) 19, 158, 228, 953, 965, 1475, 1705
AcvI CACGTG 1 cut(s) 1133
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 5 cut(s) 46, 221, 611, 1582, 2120
AfiI CCNNNNNNNGG 8 cut(s) 130, 249, 287, 363, 595, 596, 689, 1078
AflIII ACRYGT 3 cut(s) 1102, 1132, 2248
AhlI ACTAGT 1 cut(s) 2480
AjnI CCWGG 5 cut(s) 184, 579, 985, 1505, 2449
AluBI AGCT 9 cut(s) 10, 415, 1328, 1375, 1624, 1759, 2057, 2163, 2513
AluI AGCT 9 cut(s) 10, 415, 1328, 1375, 1624, 1759, 2057, 2163, 2513
Alw26I GTCTC 2 cut(s) 388, 2481
AlwI GGATC 6 cut(s) 537, 695, 1066, 1790, 1937, 2388
Ama87I CYCGRG 1 cut(s) 589
AoxI GGCC 4 cut(s) 325, 1593, 1711, 1983
ApeKI GCWGC 3 cut(s) 1747, 1794, 2426
ApoI RAATTY 7 cut(s) 19, 158, 228, 953, 965, 1475, 1705
AseI ATTAAT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 1186
AspLEI GCGC 1 cut(s) 889
AspS9I GGNCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
AsuC2I CCSGG 4 cut(s) 547, 590, 591, 706
AsuHPI GGTGA 5 cut(s) 370, 704, 829, 2103, 2430
AsuII TTCGAA 1 cut(s) 993
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
BalI TGGCCA 1 cut(s) 1713
BanI GGYRCC 2 cut(s) 709, 1111
BbrPI CACGTG 1 cut(s) 1133
BbvI GCAGC 3 cut(s) 1734, 1781, 2438
BccI CCATC 7 cut(s) 693, 782, 808, 887, 1255, 2500, 2501
BceAI ACGGC 2 cut(s) 1143, 1212
BciT130I CCWGG 5 cut(s) 186, 581, 987, 1507, 2451
BciVI GTATCC 3 cut(s) 415, 781, 1276
BcnI CCSGG 4 cut(s) 547, 590, 591, 706
BcoDI GTCTC 2 cut(s) 388, 2481
BcuI ACTAGT 1 cut(s) 2480
BfuI GTATCC 3 cut(s) 415, 781, 1276
BglI GCCNNNNNGGC 1 cut(s) 895
BglII AGATCT 2 cut(s) 729, 1975
BisI GCNGC 3 cut(s) 1748, 1795, 2427
BlsI GCNGC 3 cut(s) 1749, 1796, 2428
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 9 cut(s) 186, 547, 581, 590, 591, 706, 987, 1507, 2451
Bme18I GGWCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
BmeT110I CYCGRG 1 cut(s) 589
BmgT120I GGNCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
BmiI GGNNCC 6 cut(s) 560, 711, 872, 1113, 1645, 1772
BmrFI CCNGG 9 cut(s) 186, 547, 581, 590, 591, 706, 987, 1507, 2451
BmsI GCATC 5 cut(s) 498, 1505, 1806, 1886, 2413
Bpu14I TTCGAA 1 cut(s) 993
BpuMI CCSGG 4 cut(s) 547, 590, 591, 706
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 1 cut(s) 1133
BsaBI GATNNNNATC 2 cut(s) 818, 1820
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 2481
BsaJI CCNNGG 7 cut(s) 579, 589, 683, 705, 1506, 1714, 2280
BsaWI WCCGGW 1 cut(s) 612
BsaXI ACNNNNNCTCC 2 cut(s) 1222, 1252
Bsc4I CCNNNNNNNGG 8 cut(s) 130, 249, 287, 363, 595, 596, 689, 1078
Bse118I RCCGGY 1 cut(s) 2207
Bse1I ACTGG 5 cut(s) 298, 560, 682, 874, 2197
Bse3DI GCAATG 3 cut(s) 1996, 2130, 2346
Bse8I GATNNNNATC 2 cut(s) 818, 1820
BseBI CCWGG 5 cut(s) 186, 581, 987, 1507, 2451
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 7 cut(s) 579, 589, 683, 705, 1506, 1714, 2280
BseGI GGATG 4 cut(s) 589, 898, 1061, 1965
BseJI GATNNNNATC 2 cut(s) 818, 1820
BseLI CCNNNNNNNGG 8 cut(s) 130, 249, 287, 363, 595, 596, 689, 1078
BseMI GCAATG 3 cut(s) 1996, 2130, 2346
BseMII CTCAG 1 cut(s) 1169
BseNI ACTGG 5 cut(s) 298, 560, 682, 874, 2197
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 1734, 1781, 2438
Bsh1236I CGCG 1 cut(s) 887
BshFI GGCC 4 cut(s) 327, 1595, 1713, 1985
BshNI GGYRCC 2 cut(s) 709, 1111
BshVI ATCGAT 1 cut(s) 822
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 7 cut(s) 546, 590, 613, 705, 890, 1115, 2208
BslFI GGGAC 3 cut(s) 1268, 2107, 2270
BslI CCNNNNNNNGG 8 cut(s) 130, 249, 287, 363, 595, 596, 689, 1078
BsmAI GTCTC 2 cut(s) 388, 2481
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 3 cut(s) 1268, 2107, 2270
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 4 cut(s) 327, 1595, 1713, 1985
Bso31I GGTCTC 1 cut(s) 2481
BsoBI CYCGRG 1 cut(s) 589
Bsp119I TTCGAA 1 cut(s) 993
BspACI CCGC 6 cut(s) 456, 673, 849, 885, 1038, 1503
BspANI GGCC 4 cut(s) 327, 1595, 1713, 1985
BspCNI CTCAG 1 cut(s) 1170
BspDI ATCGAT 1 cut(s) 822
BspFNI CGCG 1 cut(s) 887
BspLI GGNNCC 6 cut(s) 560, 711, 872, 1113, 1645, 1772
BspPI GGATC 6 cut(s) 537, 695, 1066, 1790, 1937, 2388
BspT104I TTCGAA 1 cut(s) 993
BspT107I GGYRCC 2 cut(s) 709, 1111
BspTNI GGTCTC 1 cut(s) 2481
BsrDI GCAATG 3 cut(s) 1996, 2130, 2346
BsrFI RCCGGY 1 cut(s) 2207
BsrI ACTGG 5 cut(s) 298, 560, 682, 874, 2197
BssAI RCCGGY 1 cut(s) 2207
BssECI CCNNGG 7 cut(s) 579, 589, 683, 705, 1506, 1714, 2280
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 3 cut(s) 683, 1714, 2280
Bst2UI CCWGG 5 cut(s) 186, 581, 987, 1507, 2451
Bst4CI ACNGT 9 cut(s) 49, 203, 323, 382, 605, 846, 1352, 1492, 2084
Bst6I CTCTTC 7 cut(s) 65, 85, 391, 1361, 1442, 1803, 2459
BstACI GRCGYC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 993
BstC8I GCNNGC 4 cut(s) 1987, 2363, 2373, 2515
BstDEI CTNAG 4 cut(s) 125, 1178, 1577, 2333
BstEII GGTNACC 1 cut(s) 91
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 4 cut(s) 589, 898, 1061, 1965
BstFNI CGCG 1 cut(s) 887
BstHHI GCGC 1 cut(s) 889
BstMAI GTCTC 2 cut(s) 388, 2481
BstMWI GCNNNNNNNGC 8 cut(s) 311, 538, 718, 895, 1334, 1502, 1756, 1874
BstNI CCWGG 5 cut(s) 186, 581, 987, 1507, 2451
BstNSI RCATGY 4 cut(s) 1164, 1395, 1431, 2252
BstPI GGTNACC 1 cut(s) 91
BstSCI CCNGG 9 cut(s) 184, 545, 579, 588, 589, 704, 985, 1505, 2449
BstUI CGCG 1 cut(s) 887
BstV1I GCAGC 3 cut(s) 1734, 1781, 2438
BstX2I RGATCY 3 cut(s) 729, 1929, 1975
BstXI CCANNNNNNTGG 2 cut(s) 418, 1721
BstYI RGATCY 3 cut(s) 729, 1929, 1975
Bsu15I ATCGAT 1 cut(s) 822
BsuI GTATCC 3 cut(s) 415, 781, 1276
BsuRI GGCC 4 cut(s) 327, 1595, 1713, 1985
BsuTUI ATCGAT 1 cut(s) 822
BtsCI GGATG 4 cut(s) 589, 898, 1061, 1965
BtsI GCAGTG 3 cut(s) 905, 1692, 2406
BtsIMutI CAGTG 3 cut(s) 905, 1692, 2406
Cac8I GCNNGC 4 cut(s) 1987, 2363, 2373, 2515
CfoI GCGC 1 cut(s) 889
Cfr10I RCCGGY 1 cut(s) 2207
Cfr13I GGNCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
Cfr9I CCCGGG 1 cut(s) 589
ClaI ATCGAT 1 cut(s) 822
CpoI CGGWCCG 1 cut(s) 846
CseI GACGC 1 cut(s) 110
Csp6I GTAC 5 cut(s) 45, 220, 610, 1581, 2119
CspCI CAANNNNNGTGG 2 cut(s) 2067, 2102
CspI CGGWCCG 1 cut(s) 846
CviQI GTAC 5 cut(s) 45, 220, 610, 1581, 2119
DdeI CTNAG 4 cut(s) 125, 1178, 1577, 2333
EaeI YGGCCR 1 cut(s) 1711
Eam1104I CTCTTC 7 cut(s) 65, 85, 391, 1361, 1442, 1803, 2459
EarI CTCTTC 7 cut(s) 65, 85, 391, 1361, 1442, 1803, 2459
EciI GGCGGA 1 cut(s) 688
Eco130I CCWWGG 3 cut(s) 683, 1714, 2280
Eco31I GGTCTC 1 cut(s) 2481
Eco47I GGWCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
Eco72I CACGTG 1 cut(s) 1133
Eco88I CYCGRG 1 cut(s) 589
Eco91I GGTNACC 1 cut(s) 91
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO65I GGTNACC 1 cut(s) 91
EcoRI GAATTC 2 cut(s) 19, 1705
EcoRII CCWGG 5 cut(s) 184, 579, 985, 1505, 2449
EcoT14I CCWWGG 3 cut(s) 683, 1714, 2280
ErhI CCWWGG 3 cut(s) 683, 1714, 2280
Esp3I CGTCTC 1 cut(s) 388
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 3 cut(s) 1268, 2107, 2270
FauI CCCGC 1 cut(s) 1031
FauNDI CATATG 1 cut(s) 2098
Fnu4HI GCNGC 3 cut(s) 1748, 1795, 2427
FokI GGATG 4 cut(s) 596, 905, 1048, 1952
Fsp4HI GCNGC 3 cut(s) 1748, 1795, 2427
GlaI GCGC 1 cut(s) 888
GluI GCNGC 3 cut(s) 1748, 1795, 2427
HaeIII GGCC 4 cut(s) 327, 1595, 1713, 1985
HapII CCGG 7 cut(s) 546, 590, 613, 705, 890, 1115, 2208
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 889
Hin1I GRCGYC 1 cut(s) 102
Hin6I GCGC 1 cut(s) 887
HinP1I GCGC 1 cut(s) 887
HindIII AAGCTT 2 cut(s) 413, 1622
HpaII CCGG 7 cut(s) 546, 590, 613, 705, 890, 1115, 2208
HphI GGTGA 5 cut(s) 370, 704, 829, 2103, 2430
Hpy166II GTNNAC 4 cut(s) 369, 635, 1138, 2387
Hpy188I TCNGA 7 cut(s) 212, 660, 927, 1461, 1558, 2029, 2152
Hpy8I GTNNAC 4 cut(s) 369, 635, 1138, 2387
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 7 cut(s) 1076, 1102, 2123, 2260, 2371, 2445, 2543
HpyCH4III ACNGT 9 cut(s) 49, 203, 323, 382, 605, 846, 1352, 1492, 2084
HpyCH4IV ACGT 5 cut(s) 138, 741, 1102, 1132, 2154
HpyF10VI GCNNNNNNNGC 8 cut(s) 311, 538, 718, 895, 1334, 1502, 1756, 1874
HpyF3I CTNAG 4 cut(s) 125, 1178, 1577, 2333
HpySE526I ACGT 5 cut(s) 138, 741, 1102, 1132, 2154
Hsp92I GRCGYC 1 cut(s) 102
HspAI GCGC 1 cut(s) 887
LmnI GCTCC 5 cut(s) 60, 1649, 1764, 2054, 2510
Lsp1109I GCAGC 3 cut(s) 1734, 1781, 2438
LweI GCATC 5 cut(s) 498, 1505, 1806, 1886, 2413
MaeII ACGT 5 cut(s) 138, 741, 1102, 1132, 2154
MaeIII GTNAC 8 cut(s) 91, 197, 203, 742, 938, 1486, 2109, 2188
MflI RGATCY 3 cut(s) 729, 1929, 1975
MlsI TGGCCA 1 cut(s) 1713
MluNI TGGCCA 1 cut(s) 1713
MlyI GAGTC 3 cut(s) 640, 655, 1159
MmeI TCCRAC 4 cut(s) 1077, 1638, 1786, 1980
Mox20I TGGCCA 1 cut(s) 1713
MroXI GAANNNNTTC 1 cut(s) 1186
MscI TGGCCA 1 cut(s) 1713
MseI TTAA 6 cut(s) 180, 450, 750, 1590, 1731, 1958
MslI CAYNNNNRTG 3 cut(s) 1317, 1685, 2481
Msp20I TGGCCA 1 cut(s) 1713
MspI CCGG 7 cut(s) 546, 590, 613, 705, 890, 1115, 2208
MspR9I CCNGG 9 cut(s) 186, 547, 581, 590, 591, 706, 987, 1507, 2451
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 5 cut(s) 186, 581, 987, 1507, 2451
MvnI CGCG 1 cut(s) 887
MwoI GCNNNNNNNGC 8 cut(s) 311, 538, 718, 895, 1334, 1502, 1756, 1874
NciI CCSGG 4 cut(s) 547, 590, 591, 706
NdeI CATATG 1 cut(s) 2098
NlaIV GGNNCC 6 cut(s) 560, 711, 872, 1113, 1645, 1772
NmuCI GTSAC 3 cut(s) 938, 1486, 2109
NspI RCATGY 4 cut(s) 1164, 1395, 1431, 2252
NspV TTCGAA 1 cut(s) 993
PciI ACATGT 1 cut(s) 2248
PctI GAATGC 1 cut(s) 1178
PdmI GAANNNNTTC 1 cut(s) 1186
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 3 cut(s) 1749, 1796, 2428
PleI GAGTC 3 cut(s) 639, 655, 1158
PmaCI CACGTG 1 cut(s) 1133
PmlI CACGTG 1 cut(s) 1133
PpsI GAGTC 3 cut(s) 639, 655, 1158
Ppu21I YACGTR 1 cut(s) 1133
PscI ACATGT 1 cut(s) 2248
PshBI ATTAAT 1 cut(s) 450
PsiI TTATAA 1 cut(s) 1653
Psp1406I AACGTT 1 cut(s) 741
Psp6I CCWGG 5 cut(s) 184, 579, 985, 1505, 2449
PspCI CACGTG 1 cut(s) 1133
PspEI GGTNACC 1 cut(s) 91
PspGI CCWGG 5 cut(s) 184, 579, 985, 1505, 2449
PspN4I GGNNCC 6 cut(s) 560, 711, 872, 1113, 1645, 1772
PspPI GGNCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
PsrI GAACNNNNNNTAC 2 cut(s) 2195, 2227
PsuI RGATCY 3 cut(s) 729, 1929, 1975
RsaI GTAC 5 cut(s) 46, 221, 611, 1582, 2120
RsaNI GTAC 5 cut(s) 45, 220, 610, 1581, 2119
RseI CAYNNNNRTG 3 cut(s) 1317, 1685, 2481
Rsr2I CGGWCCG 1 cut(s) 846
RsrII CGGWCCG 1 cut(s) 846
SaqAI TTAA 6 cut(s) 180, 450, 750, 1590, 1731, 1958
SatI GCNGC 3 cut(s) 1748, 1795, 2427
Sau96I GGNCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
ScaI AGTACT 1 cut(s) 46
SchI GAGTC 3 cut(s) 640, 655, 1159
ScrFI CCNGG 9 cut(s) 186, 547, 581, 590, 591, 706, 987, 1507, 2451
SfaNI GCATC 5 cut(s) 498, 1505, 1806, 1886, 2413
SfuI TTCGAA 1 cut(s) 993
SinI GGWCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
SmaI CCCGGG 1 cut(s) 591
SmiMI CAYNNNNRTG 3 cut(s) 1317, 1685, 2481
SpeI ACTAGT 1 cut(s) 2480
SsiI CCGC 6 cut(s) 456, 673, 849, 885, 1038, 1503
SspI AATATT 1 cut(s) 1534
StyD4I CCNGG 9 cut(s) 184, 545, 579, 588, 589, 704, 985, 1505, 2449
StyI CCWWGG 3 cut(s) 683, 1714, 2280
TaaI ACNGT 9 cut(s) 49, 203, 323, 382, 605, 846, 1352, 1492, 2084
TaiI ACGT 5 cut(s) 141, 744, 1105, 1135, 2157
TaqI TCGA 3 cut(s) 822, 993, 1952
TatI WGTACW 1 cut(s) 44
Tru1I TTAA 6 cut(s) 180, 450, 750, 1590, 1731, 1958
Tru9I TTAA 6 cut(s) 180, 450, 750, 1590, 1731, 1958
TscAI CASTG 3 cut(s) 905, 1692, 2406
TseFI GTSAC 3 cut(s) 938, 1486, 2109
TseI GCWGC 3 cut(s) 1747, 1794, 2426
Tsp45I GTSAC 3 cut(s) 938, 1486, 2109
TspGWI ACGGA 3 cut(s) 391, 1054, 1636
TspMI CCCGGG 1 cut(s) 589
TspRI CASTG 3 cut(s) 905, 1692, 2406
Van91I CCANNNNNTGG 1 cut(s) 249
VpaK11BI GGWCC 8 cut(s) 346, 366, 680, 702, 846, 871, 882, 923
VspI ATTAAT 1 cut(s) 450
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 7 cut(s) 19, 158, 228, 953, 965, 1475, 1705
XceI RCATGY 4 cut(s) 1164, 1395, 1431, 2252
XmaI CCCGGG 1 cut(s) 589
XmnI GAANNNNTTC 1 cut(s) 1186
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.