Rroxscaffold_1G00070710

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000099
Physical Location & Seq
Forward (+)
91633919 .. 91637323
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070710.1

Sequence Viewer

Length: 2385 bp
ATGTGGTTTAAGAAAATATTGCTCATCTCGTTTGTTCTTCTTCCCTCTTGCATTTGCCTTGATACCTTTACTCTAAATCAAGCCATCAAAGACGGTGATGTGCTGGTCTCCGGCAGGGAAATCTTTGCACTTGGATTTTTCAGTGCAGGCAAGTCTAGCAACCGTTACGTTGGAGTATGGTATAACCAAATTTCTAACCAAACAGTTGTGTGGGTTGCAAACAGAGACAACCCAGTCCCTGATACCTCTGGATTGCTATCCATAAATGGAGATGGAGGCCTAGTCATCTATGGAAATAACCGAAGTTTCCCTCTTTGGTCTGCTAATGTCACTCTCTCTTCTCCAAACAATTCAATTGCCAAGCTTCTGGATACAGGAAATCTTGTCTTGCTCGAGAATGAGGATAGTCAAAAGGTTCTATGGCAAGGGTTTGATTATCCCACTGATACAATGCTTCCACTTATGAAAATTGGTTTGGACAGGCGATCTGGGCTGAACAGGTTCTTAACATCTCGGAAGTCGAAAGATGACCCAGGAATCGGGAGCTGTTCATTTAGGCTTGAACCTAGTGGATTTCCACAGATGATTTTGTACAAGGATGGAGCTCCATGGTTCCGGCCTGACCCTGCATCTTTGGTTGACCAGTCATGTTGCACTGCAACAACTTTAGTGAACAATGCAGATCAGGTATCCATTATGTCATCAGCTAAGAACAATCAATCAAATTTCTCAAGGACGGTGTTAGATGAATCAGGAACATTAGGACGGTTCACGTGGAATGATCGAGCGCAAAAATGGATAGAACTTAGGTCTGATCCAATAGAGCGTTGCGATATTTATGGACAATGTGGTCCAAATGGTATTTGCGATCCCTATGCTACTGATACTATAGATTGCACCTGCCTTCCGGGGTTTGAACCCAAGTCGCCAACGGATTGGTACTCGAGAGATGGGACAGATGGGTGTGTAAAGAAAAAAGGAGTATCTACATGTCGAAATGGGGAAAAATTTGTGCCTCTGGAAAGTAAGAAAATACCAGACTTGTCTAGGGCACTTGTTAATATGAATGTGAGTATGAAAGCGTGCGAGCAAGAATGCTTGACAAATTGTTCTTGCGTTGCATACAGTAGCAGCGACAAGACTGGATGTGTGACATGGTATGGGGAATTGATGGACACAAGGGCGCTGCCAAATACTGGTCAAACTTTATATGTACGAGTTGATGCAATTGTCTTTGCTCAATATGCAAAGAAGTCAAATAATTCTCTTAGCAAGAAGGAAAAGCTGGCAATTTCACTACTATCTGTTTTAGCGCTATTTCTACTTGTTTTCCTTGTCTATTGGTTACTGAGGATGAAGAGAAAAGGTAAAAGAAGACGAGATGAATATTCATTTAGCTCCTTCAAGAAAGCTACTGGTGGAACGGAACCTGATGAAAGTAGCATAAGCTCAGATTTGCCATTCTTCGACCTAACAACCATAGCAGCAGCCACAGACAACTTCTCTCTTGCAAACAAGCTTGGAACAGGCGGTTTTGGCCCCGTCTATAAGGGTGTACTTTTTAATGGAAAGGAAATAGCAGTGAAAAGATTATCGAAGACCTCGGGTCAAGGAATTGAAGAGTTTAAAAATGAGGTTTTGTTGATAGCGAAACTCCAACATCGGAACCTTGTGAGGATATTAGATGAAACCAACAGAGCACTTTTAGATTGGACAAGACGCTTTGAGATTATCCACGGAATTACTAGAGGAATTATATATCTTCATCAAGATTCAAGATTAAGAATTATCCACCGAGACCTAAAGGCCAGCAATGTTCTACTAGATGCTTCTATGAACCCCAAAATTGCAGATTTTGGTATGGCTAGAATTTTTAAGGGAGATCAAAATGAAGCAAATACAAAGCGTGTGGTTGGAACATATGGCTATATGTCACCAGAGTATGCAATGCGAGGAAGGTTTTCGGTGAAGTCAGATGTATATAGCTTTGGCGTTTTACTAATAGAAATCATTACCGGGAGAAAGAATAATGGCTTTTACCATGAGGAACATCCCGACTCAAATTTAGTTGGCCATGTTTGGAACTTGTGGCGAGAAGGTAAAGTCTTGCAAATAGTTGATTCGTCCATCGGTGAATCATACCCCGTCAATGAAGTTCTGAAATGTATTCAAATTGCCTTCTTGTGCTTGCAAGAGTATCCAGCTGACCGGCCAACCATGTCAGAAGTTCTTTTCATGCTAGGTAACAATGCGGCTCTTCCTTCACCACGAAAACCTGCATTCTTATTGGAGAGAAGTAGCAGTTCTATGGGTGGCAAGTTGTCAACCCGTGAAGGATCTAATTCTCTAAATGATTTCACATGTAGTGTGGTGGAAGCTCGGTAG

Protein Analysis

794

Amino Acids

88.46

Weight (kDa)

8.64

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 68 - 173 2.4e-34 D-mannose binding lectin
S_locus_glycop PF00954 220 - 309 1e-19 S-locus glycoprotein domain
PAN_2 PF08276 331 - 393 1.4e-16 PAN-like domain
Pkinase PF00069 503 - 562 2e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 503 - 561 7.4e-07 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 567 - 744 1.4e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 576 - 744 2.8e-27 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 908
AasI GACNNNNNNGTC 2 cut(s) 233, 849
Acc36I ACCTGC 2 cut(s) 908, 2284
AccB7I CCANNNNNTGG 1 cut(s) 1196
AciI CCGC 2 cut(s) 1530, 2252
AclWI GGATC 3 cut(s) 809, 863, 2344
AcoI YGGCCR 2 cut(s) 2071, 2210
AcsI RAATTY 5 cut(s) 189, 724, 1007, 1869, 2062
AcvI CACGTG 1 cut(s) 774
AfaI GTAC 4 cut(s) 593, 941, 1215, 1557
AfeI AGCGCT 1 cut(s) 1314
AfiI CCNNNNNNNGG 2 cut(s) 539, 1196
AflIII ACRYGT 2 cut(s) 989, 2360
AgsI TTSAA 7 cut(s) 354, 563, 917, 1405, 1619, 1776, 2171
AhdI GACNNNNNGTC 1 cut(s) 1605
AjnI CCWGG 1 cut(s) 532
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
Alw21I GWGCWC 2 cut(s) 607, 1702
Alw26I GTCTC 3 cut(s) 112, 219, 1791
AlwI GGATC 3 cut(s) 809, 863, 2344
AlwNI CAGNNNCTG 1 cut(s) 239
Ama87I CYCGRG 3 cut(s) 392, 943, 1603
Aor51HI AGCGCT 1 cut(s) 1314
AoxI GGCC 6 cut(s) 277, 617, 1537, 1806, 2071, 2210
ApeKI GCWGC 4 cut(s) 1131, 1186, 1484, 1487
ApoI RAATTY 5 cut(s) 189, 724, 1007, 1869, 2062
Asp700I GAANNNNTTC 2 cut(s) 500, 1960
AspLEI GCGC 3 cut(s) 790, 1186, 1315
AspS9I GGNCC 2 cut(s) 851, 1538
AsuC2I CCSGG 2 cut(s) 909, 2017
AsuHPI GGTGA 5 cut(s) 107, 1926, 1978, 2144, 2256
AvaI CYCGRG 3 cut(s) 392, 943, 1603
AvaII GGWCC 1 cut(s) 851
BaeGI GKGCMC 1 cut(s) 1054
BalI TGGCCA 1 cut(s) 2073
BanII GRGCYC 1 cut(s) 607
BbrPI CACGTG 1 cut(s) 774
BbsI GAAGAC 2 cut(s) 1381, 1604
Bbv12I GWGCWC 2 cut(s) 607, 1702
BbvI GCAGC 4 cut(s) 1143, 1173, 1496, 1499
BccI CCATC 7 cut(s) 92, 266, 593, 944, 953, 1165, 2135
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 1 cut(s) 534
BciVI GTATCC 3 cut(s) 364, 700, 2208
BcnI CCSGG 2 cut(s) 909, 2017
BcoDI GTCTC 3 cut(s) 112, 219, 1791
BfaI CTAG 8 cut(s) 156, 281, 567, 1047, 1746, 1823, 1866, 2240
BfmI CTRYAG 1 cut(s) 888
BfoI RGCGCY 2 cut(s) 1187, 1316
BfuAI ACCTGC 2 cut(s) 908, 2284
BfuI GTATCC 3 cut(s) 364, 700, 2208
BisI GCNGC 5 cut(s) 1132, 1187, 1485, 1488, 2253
BlsI GCNGC 5 cut(s) 1133, 1188, 1486, 1489, 2254
Bme1390I CCNGG 3 cut(s) 534, 909, 2017
Bme18I GGWCC 1 cut(s) 851
BmeRI GACNNNNNGTC 1 cut(s) 1605
BmeT110I CYCGRG 3 cut(s) 392, 943, 1603
BmgT120I GGNCC 2 cut(s) 851, 1538
BmiI GGNNCC 4 cut(s) 614, 1428, 1540, 1667
BmrFI CCNGG 3 cut(s) 534, 909, 2017
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 3 cut(s) 638, 1213, 1816
BmuI ACTGGG 1 cut(s) 227
BpiI GAAGAC 2 cut(s) 1381, 1604
BpuEI CTTGAG 1 cut(s) 715
BpuMI CCSGG 2 cut(s) 909, 2017
BsaAI YACGTR 1 cut(s) 774
BsaBI GATNNNNATC 1 cut(s) 256
BsaI GGTCTC 2 cut(s) 112, 1791
BsaJI CCNNGG 5 cut(s) 532, 608, 908, 1602, 1735
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 1196
Bse118I RCCGGY 1 cut(s) 2208
Bse1I ACTGG 5 cut(s) 233, 643, 1147, 1201, 1420
Bse3DI GCAATG 2 cut(s) 1819, 1953
Bse8I GATNNNNATC 1 cut(s) 256
BseBI CCWGG 1 cut(s) 534
BseDI CCNNGG 5 cut(s) 532, 608, 908, 1602, 1735
BseGI GGATG 4 cut(s) 604, 1151, 1359, 2050
BseJI GATNNNNATC 1 cut(s) 256
BseLI CCNNNNNNNGG 2 cut(s) 539, 1196
BseMI GCAATG 2 cut(s) 1819, 1953
BseMII CTCAG 2 cut(s) 1340, 1464
BseNI ACTGG 5 cut(s) 233, 643, 1147, 1201, 1420
BseSI GKGCMC 1 cut(s) 1054
BseXI GCAGC 4 cut(s) 1143, 1173, 1496, 1499
BsgI GTGCAG 1 cut(s) 165
BshFI GGCC 6 cut(s) 279, 619, 1539, 1808, 2073, 2212
BsiHKAI GWGCWC 2 cut(s) 607, 1702
BsiHKCI CYCGRG 3 cut(s) 392, 943, 1603
BsiSI CCGG 5 cut(s) 111, 616, 908, 2016, 2209
BslFI GGGAC 2 cut(s) 221, 967
BslI CCNNNNNNNGG 2 cut(s) 539, 1196
BsmAI GTCTC 3 cut(s) 112, 219, 1791
BsmFI GGGAC 2 cut(s) 221, 967
BsmI GAATGC 2 cut(s) 1100, 2279
BsnI GGCC 6 cut(s) 279, 619, 1539, 1808, 2073, 2212
Bso31I GGTCTC 2 cut(s) 112, 1791
BsoBI CYCGRG 3 cut(s) 392, 943, 1603
Bsp1286I GDGCHC 3 cut(s) 607, 1054, 1702
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 2 cut(s) 1530, 2252
BspANI GGCC 6 cut(s) 279, 619, 1539, 1808, 2073, 2212
BspCNI CTCAG 2 cut(s) 1341, 1463
BspLI GGNNCC 4 cut(s) 614, 1428, 1540, 1667
BspMI ACCTGC 2 cut(s) 908, 2284
BspPI GGATC 3 cut(s) 809, 863, 2344
BspQI GCTCTTC 1 cut(s) 2262
BspTNI GGTCTC 2 cut(s) 112, 1791
BsrDI GCAATG 2 cut(s) 1819, 1953
BsrFI RCCGGY 1 cut(s) 2208
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 5 cut(s) 233, 643, 1147, 1201, 1420
BssAI RCCGGY 1 cut(s) 2208
BssECI CCNNGG 5 cut(s) 532, 608, 908, 1602, 1735
BssMI GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
BssT1I CCWWGG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 6 cut(s) 95, 164, 205, 739, 768, 1127
Bst6I CTCTTC 4 cut(s) 343, 1352, 1614, 2262
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 774
BstC8I GCNNGC 6 cut(s) 148, 1084, 1088, 1287, 1810, 2189
BstDEI CTNAG 5 cut(s) 708, 806, 1268, 1349, 1450
BstDSI CCRYGG 2 cut(s) 608, 1735
BstF5I GGATG 4 cut(s) 604, 1151, 1359, 2050
BstH2I RGCGCY 2 cut(s) 1187, 1316
BstHHI GCGC 3 cut(s) 790, 1186, 1315
BstKTI GATC 7 cut(s) 488, 685, 784, 817, 871, 1885, 2339
BstMAI GTCTC 3 cut(s) 112, 219, 1791
BstMBI GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
BstMWI GCNNNNNNNGC 4 cut(s) 156, 490, 1244, 1536
BstNI CCWGG 1 cut(s) 534
BstNSI RCATGY 2 cut(s) 993, 2364
BstSCI CCNGG 3 cut(s) 532, 907, 2015
BstSFI CTRYAG 1 cut(s) 888
BstSLI GKGCMC 1 cut(s) 1054
BstV1I GCAGC 4 cut(s) 1143, 1173, 1496, 1499
BstV2I GAAGAC 2 cut(s) 1381, 1604
BstX2I RGATCY 1 cut(s) 2336
BstXI CCANNNNNNTGG 2 cut(s) 367, 936
BstYI RGATCY 1 cut(s) 2336
BsuI GTATCC 3 cut(s) 364, 700, 2208
BsuRI GGCC 6 cut(s) 279, 619, 1539, 1808, 2073, 2212
BtgI CCRYGG 2 cut(s) 608, 1735
BtsCI GGATG 4 cut(s) 604, 1151, 1359, 2050
BtsI GCAGTG 2 cut(s) 654, 1587
BtsIMutI CAGTG 4 cut(s) 148, 441, 654, 1587
BveI ACCTGC 2 cut(s) 908, 2284
Cac8I GCNNGC 6 cut(s) 148, 1084, 1088, 1287, 1810, 2189
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 3 cut(s) 790, 1186, 1315
Cfr10I RCCGGY 1 cut(s) 2208
Cfr13I GGNCC 2 cut(s) 851, 1538
CseI GACGC 1 cut(s) 1728
Csp6I GTAC 4 cut(s) 592, 940, 1214, 1556
CspCI CAANNNNNGTGG 2 cut(s) 1890, 1925
CviAII CATG 9 cut(s) 609, 648, 990, 1155, 2042, 2075, 2218, 2236, 2361
CviQI GTAC 4 cut(s) 592, 940, 1214, 1556
DdeI CTNAG 5 cut(s) 708, 806, 1268, 1349, 1450
DpnI GATC 7 cut(s) 487, 684, 783, 816, 870, 1884, 2338
DpnII GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
DraI TTTAAA 1 cut(s) 1627
DrdI GACNNNNNNGTC 2 cut(s) 233, 849
DriI GACNNNNNGTC 1 cut(s) 1605
DseDI GACNNNNNNGTC 2 cut(s) 233, 849
EaeI YGGCCR 2 cut(s) 2071, 2210
Eam1104I CTCTTC 4 cut(s) 343, 1352, 1614, 2262
Eam1105I GACNNNNNGTC 1 cut(s) 1605
EarI CTCTTC 4 cut(s) 343, 1352, 1614, 2262
Ecl136II GAGCTC 1 cut(s) 605
Eco130I CCWWGG 1 cut(s) 608
Eco147I AGGCCT 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 607
Eco31I GGTCTC 2 cut(s) 112, 1791
Eco47I GGWCC 1 cut(s) 851
Eco47III AGCGCT 1 cut(s) 1314
Eco53kI GAGCTC 1 cut(s) 605
Eco72I CACGTG 1 cut(s) 774
Eco88I CYCGRG 3 cut(s) 392, 943, 1603
EcoICRI GAGCTC 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 608
EcoT38I GRGCYC 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 608
FaeI CATG 9 cut(s) 612, 651, 993, 1158, 2045, 2078, 2221, 2239, 2364
FaqI GGGAC 2 cut(s) 221, 967
FatI CATG 9 cut(s) 608, 647, 989, 1154, 2041, 2074, 2217, 2235, 2360
FauNDI CATATG 1 cut(s) 1921
Fnu4HI GCNGC 5 cut(s) 1132, 1187, 1485, 1488, 2253
FokI GGATG 4 cut(s) 611, 1158, 1366, 2037
FriOI GRGCYC 1 cut(s) 607
Fsp4HI GCNGC 5 cut(s) 1132, 1187, 1485, 1488, 2253
FspBI CTAG 8 cut(s) 156, 281, 567, 1047, 1746, 1823, 1866, 2240
GlaI GCGC 3 cut(s) 789, 1185, 1314
GluI GCNGC 5 cut(s) 1132, 1187, 1485, 1488, 2253
HaeII RGCGCY 2 cut(s) 1187, 1316
HaeIII GGCC 6 cut(s) 279, 619, 1539, 1808, 2073, 2212
HapII CCGG 5 cut(s) 111, 616, 908, 2016, 2209
HgaI GACGC 1 cut(s) 1728
HhaI GCGC 3 cut(s) 790, 1186, 1315
Hin1II CATG 9 cut(s) 612, 651, 993, 1158, 2045, 2078, 2221, 2239, 2364
Hin6I GCGC 3 cut(s) 788, 1184, 1313
HinP1I GCGC 3 cut(s) 788, 1184, 1313
HincII GTYRAC 2 cut(s) 640, 2325
HindII GTYRAC 2 cut(s) 640, 2325
HindIII AAGCTT 2 cut(s) 362, 1517
HinfI GANTC 6 cut(s) 537, 749, 1772, 2057, 2120, 2135
HpaII CCGG 5 cut(s) 111, 616, 908, 2016, 2209
HphI GGTGA 5 cut(s) 107, 1926, 1978, 2144, 2256
Hpy166II GTNNAC 5 cut(s) 640, 673, 771, 1556, 2325
Hpy188I TCNGA 7 cut(s) 516, 814, 1453, 1665, 1975, 2160, 2224
Hpy8I GTNNAC 5 cut(s) 640, 673, 771, 1556, 2325
HpyAV CCTTC 8 cut(s) 914, 1270, 1411, 1950, 2090, 2188, 2271, 2327
HpyCH4III ACNGT 6 cut(s) 95, 164, 205, 739, 768, 1127
HpyCH4IV ACGT 2 cut(s) 168, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 490, 1244, 1536
HpyF3I CTNAG 5 cut(s) 708, 806, 1268, 1349, 1450
HpySE526I ACGT 2 cut(s) 168, 773
Hsp92II CATG 9 cut(s) 612, 651, 993, 1158, 2045, 2078, 2221, 2239, 2364
HspAI GCGC 3 cut(s) 788, 1184, 1313
Kzo9I GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
LguI GCTCTTC 1 cut(s) 2262
LmnI GCTCC 4 cut(s) 543, 602, 610, 1403
Lsp1109I GCAGC 4 cut(s) 1143, 1173, 1496, 1499
LweI GCATC 3 cut(s) 638, 1213, 1816
MaeI CTAG 8 cut(s) 156, 281, 567, 1047, 1746, 1823, 1866, 2240
MaeII ACGT 2 cut(s) 168, 773
MaeIII GTNAC 6 cut(s) 164, 328, 1150, 1344, 1932, 2243
MalI GATC 7 cut(s) 487, 684, 783, 816, 870, 1884, 2338
MboI GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
MfeI CAATTG 2 cut(s) 354, 1227
MflI RGATCY 1 cut(s) 2336
MhlI GDGCHC 3 cut(s) 607, 1054, 1702
MlsI TGGCCA 1 cut(s) 2073
MluNI TGGCCA 1 cut(s) 2073
MlyI GAGTC 1 cut(s) 2051
MmeI TCCRAC 3 cut(s) 151, 1681, 1894
Mox20I TGGCCA 1 cut(s) 2073
MroXI GAANNNNTTC 2 cut(s) 500, 1960
MscI TGGCCA 1 cut(s) 2073
MseI TTAA 7 cut(s) 9, 506, 1059, 1563, 1626, 1781, 1875
Msp20I TGGCCA 1 cut(s) 2073
MspA1I CMGCKG 1 cut(s) 2204
MspI CCGG 5 cut(s) 111, 616, 908, 2016, 2209
MspR9I CCNGG 3 cut(s) 534, 909, 2017
MunI CAATTG 2 cut(s) 354, 1227
Mva1269I GAATGC 2 cut(s) 1100, 2279
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 4 cut(s) 156, 490, 1244, 1536
NciI CCSGG 2 cut(s) 909, 2017
NcoI CCATGG 1 cut(s) 608
NdeI CATATG 1 cut(s) 1921
NdeII GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
NlaIII CATG 9 cut(s) 612, 651, 993, 1158, 2045, 2078, 2221, 2239, 2364
NlaIV GGNNCC 4 cut(s) 614, 1428, 1540, 1667
NmuCI GTSAC 3 cut(s) 328, 1150, 1932
NspI RCATGY 2 cut(s) 993, 2364
PaeR7I CTCGAG 2 cut(s) 392, 943
PaqCI CACCTGC 1 cut(s) 908
PceI AGGCCT 1 cut(s) 279
PciI ACATGT 2 cut(s) 989, 2360
PciSI GCTCTTC 1 cut(s) 2262
PctI GAATGC 2 cut(s) 1100, 2279
PdmI GAANNNNTTC 2 cut(s) 500, 1960
PfeI GAWTC 5 cut(s) 537, 749, 1772, 2120, 2135
PflMI CCANNNNNTGG 1 cut(s) 1196
PkrI GCNGC 5 cut(s) 1133, 1188, 1486, 1489, 2254
PleI GAGTC 1 cut(s) 2051
PmaCI CACGTG 1 cut(s) 774
PmlI CACGTG 1 cut(s) 774
PpsI GAGTC 1 cut(s) 2051
Ppu21I YACGTR 1 cut(s) 774
PscI ACATGT 2 cut(s) 989, 2360
Psp124BI GAGCTC 1 cut(s) 607
Psp6I CCWGG 1 cut(s) 532
PspCI CACGTG 1 cut(s) 774
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 4 cut(s) 614, 1428, 1540, 1667
PspPI GGNCC 2 cut(s) 851, 1538
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 2336
PvuII CAGCTG 1 cut(s) 2204
RsaI GTAC 4 cut(s) 593, 941, 1215, 1557
RsaNI GTAC 4 cut(s) 592, 940, 1214, 1556
SacI GAGCTC 1 cut(s) 607
SapI GCTCTTC 1 cut(s) 2262
SaqAI TTAA 7 cut(s) 9, 506, 1059, 1563, 1626, 1781, 1875
SatI GCNGC 5 cut(s) 1132, 1187, 1485, 1488, 2253
Sau3AI GATC 7 cut(s) 485, 682, 781, 814, 868, 1882, 2336
Sau96I GGNCC 2 cut(s) 851, 1538
SchI GAGTC 1 cut(s) 2051
ScrFI CCNGG 3 cut(s) 534, 909, 2017
SduI GDGCHC 3 cut(s) 607, 1054, 1702
SfaNI GCATC 3 cut(s) 638, 1213, 1816
SfcI CTRYAG 1 cut(s) 888
Sfr274I CTCGAG 2 cut(s) 392, 943
SinI GGWCC 1 cut(s) 851
SlaI CTCGAG 2 cut(s) 392, 943
SmlI CTYRAG 3 cut(s) 392, 730, 943
SmoI CTYRAG 3 cut(s) 392, 730, 943
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 2 cut(s) 1530, 2252
SspI AATATT 2 cut(s) 18, 1388
SspMI CTAG 8 cut(s) 156, 281, 567, 1047, 1746, 1823, 1866, 2240
SstI GAGCTC 1 cut(s) 607
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 532, 907, 2015
StyI CCWWGG 1 cut(s) 608
TaaI ACNGT 6 cut(s) 95, 164, 205, 739, 768, 1127
TaiI ACGT 2 cut(s) 171, 776
TaqI TCGA 7 cut(s) 393, 521, 784, 944, 994, 1467, 1595
TatI WGTACW 2 cut(s) 591, 1555
TauI GCSGC 1 cut(s) 2255
TfiI GAWTC 5 cut(s) 537, 749, 1772, 2120, 2135
Tru1I TTAA 7 cut(s) 9, 506, 1059, 1563, 1626, 1781, 1875
Tru9I TTAA 7 cut(s) 9, 506, 1059, 1563, 1626, 1781, 1875
TscAI CASTG 4 cut(s) 148, 448, 661, 1587
TseFI GTSAC 3 cut(s) 328, 1150, 1932
TseI GCWGC 4 cut(s) 1131, 1186, 1484, 1487
Tsp45I GTSAC 3 cut(s) 328, 1150, 1932
TspGWI ACGGA 3 cut(s) 947, 1439, 1752
TspRI CASTG 4 cut(s) 148, 448, 661, 1587
Van91I CCANNNNNTGG 1 cut(s) 1196
VpaK11BI GGWCC 1 cut(s) 851
XapI RAATTY 5 cut(s) 189, 724, 1007, 1869, 2062
XceI RCATGY 2 cut(s) 993, 2364
XhoI CTCGAG 2 cut(s) 392, 943
XmnI GAANNNNTTC 2 cut(s) 500, 1960
XspI CTAG 8 cut(s) 156, 281, 567, 1047, 1746, 1823, 1866, 2240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.