Rw2G022580

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000099
Physical Location & Seq
Forward (+)
32245311 .. 32248727
3417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022580.1

Sequence Viewer

Length: 2535 bp
ATGTATTCTATTCAATGGTCTATGATCACTGCATTGCTCATCTTCCTCCTTCTTTATCTCCCCTCTTGCATTTCCATTGATTCCATAACTCCAAACCAAACCATCAAAGACGGCGACGTTTTAGTCTCGAGCAAGAAGCTCTTTGCGCTAGGGTTCTTCAGCCCTGGAAATTCTGGAAAACGTTACTTTGGAGTTTGGTTTAACAAAGTTCCAGAGCAGACCATTGTTTGGGTGGCAAATAGGGACAACCCAATCAATGATTCCTCTGGACTTCTAGTGATCGATGCAGATGGAGGCCTTGTCATATATGGAAAGGACAGAAAACTCCCTCTTTGGTCCACTAATGTTACTCTCTCTTATCCAAAAAACTCCATGGCCAAACTGTTGGATACAGGGAACTTTGTTTTGTTTGAGAATGGTCGGAGCCTGTGGGAAGGGTTTGATTATCCCACAAATACAATGCTTCCCTTTATGAAAATTGGGCTGAACCGGCGATCCGGCCTTAACCGGTTCCTAACTTCTTCGAAGTCCCGAGATGACCTGGGAACAGGGAATTTCTCAGCTAGGATTGATCCAGATGGGTTTCCGCAGATGTTTCTGTACAAAGGTCTAGCTCCCCGGTGGCGAATCGGATCTTGGATCGGGCACAGATGGAGTGGTGTCCCCATAATGGATGGCATTAAGTTCGACTCAATCTTCGACGTTAGTTTTGTGAACAATCAAGATGAGCTATACGTCATGGATAGAATTAAGGATGACTCAATCTTCTCAAGGATGGTGCTTGATGAATCGGGAATCCTCGAACGGTCCATGTGGCAAGATCAGCAACGCCAATGGAGCAAGTATTGGTCTGGCCCGGTAGAGCTATGTGACAACTATGGAGAGTGTGGTCCAAATAGCAACTGTGACCCTTACATATACAATGATGGTATGTTCGAGTGTGCATGCCTACCAGGGTTTGAACCAAAGTCGAACCATGCATGGTACTTGAAAGATGGCTCGAGTGGGTGTGTGAGGAAAAAAGGAGCATCTGTGTGTAAAAACGGAGAAGGGTTCGTGAAGGTGTCACCAGTAAAGGCACCAGACTCATCTACTGCACATGTAAACTTGACCTTGGGTCTGAAAGAATGTGAAAAGGAATGCTTAAGGAATTGTTCTTGCATGGCATACTCGAGTGCAAGTGGCCCGCAAGAAAGGAATGGATGCGTGACATGGTACGGGGACTTGATGGATACAAGGACTTACACGAGTGTCACTCAAGAATTATATGTTCGAGTTGATGCAATTGAATTAGCTAAACACGCAAGGAAATCGAATGGTTCTCTTAGAAAGAAGGGAAAGCTGGCAATTACTATAACATCTATTTTAGTGTTCTTTCTTGTGATCTCTATAGCATATTGGTCGGTAAAGAAGAAGAGAAAAGGTACGGAAAGACAAAATCTATTTTCATTTGGTGTGACCACACCTTCAGTCTACTTGGAAGATTTTCCTAGTAGAACAGATCCTGATGATAGCAGATTAAAGTCGGATATACCTTTCTTTGATCTAAGCACCATATCTGCAGCCACAAACAATTTCTCTTATTCGAACAGGCTTGGAGAAGGCGGATTCGGCCCCGTTTATAAGGGAGTGCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTAAGCAAAAATTCTGGCCAGGGAAATGAAGAGTTCAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTGAGAATCTTAGGCTATTGCATTCAAGATGAAGAGAAGATGCTAATCTATGAATACATGCCTAACAAAAGCCTGGACTCATTCATTTTCAATGAAATGAAAAGAACATCTCTGGATTGGACAAGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGGATCTTGTATCTTCATCAAGATTCGAGATTAAGAATCATCCACAGAGACCTAAAGGCCAGCAATATTCTACTAGATTATGATATGAACCCAAAAATTGCAGATTTTGGTGTGGCTAGAATATTCGGAGCAAACCAAATTGCAGCAAATACAAATCGCGTGGTTGGAACATATGGTTACATGTCACCGGAGTATGCGATGGAAGGAGTATTTTCAGTGAAGTCTGATGTATACAGTTTCGGTGTTCTACTGCTAGAAATCGTTACTGGCAGAAAGAACACCGGTTACTACTATGATAATCCTGACTCAAATTTGGTTGGACATGTTTGGAACTTGTGGAAAGAAGGCCGAGCCTTGGAAATCGTCGACACATCTTTGGGTGAATCCTACCCCATAAGTGAAGTTCTAAGGTGCATTCAAATTGCGCTCTTGTGCGTGCAAGAGTACGCAAATGACAGGCCAACCATGTCAAAAGTTGTGTCTGTGTTAGGCAACGATGCAGATCTTCCTTCACCAAGAAATCCTGGATTTCTAGTGAAGAGAAGTAACTATACCAGTGGAGACCCCAGTACTAGTACTGGAGATGCTAATTCCATAAATTATGTTACAAGCACTATAGTTGAAGCTCGATAA

Protein Analysis

844

Amino Acids

94.81

Weight (kDa)

8.14

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 176 2.6e-30 D-mannose binding lectin
S_locus_glycop PF00954 208 - 324 7.8e-25 S-locus glycoprotein domain
PAN_2 PF08276 346 - 412 1.7e-18 PAN-like domain
PK_Tyr_Ser-Thr PF07714 528 - 797 9.3e-49 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 528 - 794 1e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1623
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 1078
AccB7I CCANNNNNTGG 1 cut(s) 228
AccI GTMKAC 3 cut(s) 1473, 2133, 2268
AccII CGCG 1 cut(s) 2061
AciI CCGC 3 cut(s) 587, 1187, 1605
AclI AACGTT 1 cut(s) 181
AclWI GGATC 6 cut(s) 489, 566, 640, 647, 1496, 1913
AcoI YGGCCR 2 cut(s) 375, 1681
AcsI RAATTY 4 cut(s) 169, 553, 1675, 2212
AcuI CTGAAG 2 cut(s) 142, 1452
AfaI GTAC 7 cut(s) 602, 986, 1217, 1426, 2348, 2473, 2479
AfiI CCNNNNNNNGG 3 cut(s) 228, 670, 2257
AflII CTTAAG 1 cut(s) 1144
AflIII ACRYGT 3 cut(s) 1099, 2082, 2224
AgeI ACCGGT 2 cut(s) 507, 2183
AhdI GACNNNNNGTC 1 cut(s) 1116
AhlI ACTAGT 1 cut(s) 2474
AjnI CCWGG 6 cut(s) 163, 540, 952, 1683, 1818, 2425
AjuI GAANNNNNNNTTGG 4 cut(s) 244, 276, 619, 651
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 8 cut(s) 139, 563, 614, 730, 865, 1295, 1342, 2528
AluI AGCT 8 cut(s) 139, 563, 614, 730, 865, 1295, 1342, 2528
Alw26I GTCTC 3 cut(s) 130, 1944, 2457
AlwI GGATC 6 cut(s) 489, 566, 640, 647, 1496, 1913
AlwNI CAGNNNCTG 1 cut(s) 1505
Ama87I CYCGRG 4 cut(s) 127, 531, 1000, 1171
ApeKI GCWGC 2 cut(s) 1562, 2045
ApoI RAATTY 4 cut(s) 169, 553, 1675, 2212
ArsI GACNNNNNNTTYG 6 cut(s) 680, 692, 712, 724, 2366, 2398
AsiGI ACCGGT 2 cut(s) 507, 2183
Asp700I GAANNNNTTC 1 cut(s) 1485
AspLEI GCGC 2 cut(s) 148, 2329
AspS9I GGNCC 6 cut(s) 336, 807, 854, 890, 1184, 1613
AsuC2I CCSGG 2 cut(s) 619, 857
AsuHPI GGTGA 4 cut(s) 1059, 2079, 2294, 2406
AsuII TTCGAA 2 cut(s) 524, 1586
AvaI CYCGRG 4 cut(s) 127, 531, 1000, 1171
AvaII GGWCC 3 cut(s) 336, 807, 890
BaeGI GKGCMC 1 cut(s) 648
BalI TGGCCA 2 cut(s) 377, 1683
BanI GGYRCC 1 cut(s) 1078
BarI GAAGNNNNNNTAC 2 cut(s) 2437, 2469
BauI CACGAG 1 cut(s) 1246
BbvI GCAGC 2 cut(s) 1574, 2057
BceAI ACGGC 2 cut(s) 127, 1640
BcgI CGANNNNNNTGC 6 cut(s) 667, 701, 1293, 1327, 1383, 1417
BciT130I CCWGG 6 cut(s) 165, 542, 954, 1685, 1820, 2427
BciVI GTATCC 2 cut(s) 382, 1225
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 2 cut(s) 619, 857
BcoDI GTCTC 3 cut(s) 130, 1944, 2457
BcuI ACTAGT 1 cut(s) 2474
BfmI CTRYAG 3 cut(s) 1389, 1560, 2517
BfrI CTTAAG 1 cut(s) 1144
BfuI GTATCC 2 cut(s) 382, 1225
BglII AGATCT 1 cut(s) 2404
BisI GCNGC 2 cut(s) 1563, 2046
BlsI GCNGC 2 cut(s) 1564, 2047
BmcAI AGTACT 2 cut(s) 2473, 2479
Bme1390I CCNGG 8 cut(s) 165, 542, 619, 857, 954, 1685, 1820, 2427
Bme18I GGWCC 3 cut(s) 336, 807, 890
BmeRI GACNNNNNGTC 1 cut(s) 1116
BmeT110I CYCGRG 4 cut(s) 127, 531, 1000, 1171
BmgT120I GGNCC 6 cut(s) 336, 807, 854, 890, 1184, 1613
BmiI GGNNCC 5 cut(s) 425, 512, 1080, 1615, 1742
BmrFI CCNGG 8 cut(s) 165, 542, 619, 857, 954, 1685, 1820, 2427
BmrI ACTGGG 1 cut(s) 2463
BmsI GCATC 7 cut(s) 274, 1037, 1193, 1270, 1776, 2389, 2476
BmuI ACTGGG 1 cut(s) 2463
BplI GAGNNNNNCTC 2 cut(s) 1240, 1272
BpmI CTGGAG 1 cut(s) 2502
Bpu14I TTCGAA 2 cut(s) 524, 1586
BpuEI CTTGAG 2 cut(s) 754, 1242
BpuMI CCSGG 2 cut(s) 619, 857
Bsa29I ATCGAT 1 cut(s) 282
BsaBI GATNNNNATC 2 cut(s) 1790, 2403
BsaI GGTCTC 2 cut(s) 1944, 2457
BsaJI CCNNGG 8 cut(s) 163, 372, 541, 617, 953, 1113, 1684, 2256
BsaWI WCCGGW 3 cut(s) 507, 2089, 2183
BsaXI ACNNNNNCTCC 4 cut(s) 285, 315, 1017, 1047
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 670, 2257
Bse118I RCCGGY 3 cut(s) 489, 507, 2183
Bse1I ACTGG 5 cut(s) 1070, 2173, 2457, 2469, 2485
Bse3DI GCAATG 2 cut(s) 32, 1893
Bse8I GATNNNNATC 2 cut(s) 1790, 2403
BseBI CCWGG 6 cut(s) 165, 542, 954, 1685, 1820, 2427
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 8 cut(s) 163, 372, 541, 617, 953, 1113, 1684, 2256
BseGI GGATG 5 cut(s) 679, 760, 780, 1208, 1941
BseJI GATNNNNATC 2 cut(s) 1790, 2403
BseLI CCNNNNNNNGG 3 cut(s) 228, 670, 2257
BseMI GCAATG 2 cut(s) 32, 1893
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 5 cut(s) 1070, 2173, 2457, 2469, 2485
BseSI GKGCMC 1 cut(s) 648
BseXI GCAGC 2 cut(s) 1574, 2057
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 2061
BshNI GGYRCC 1 cut(s) 1078
BshTI ACCGGT 2 cut(s) 507, 2183
BshVI ATCGAT 1 cut(s) 282
BsiHKCI CYCGRG 4 cut(s) 127, 531, 1000, 1171
BsiSI CCGG 7 cut(s) 490, 498, 508, 619, 857, 2090, 2184
BslFI GGGAC 4 cut(s) 257, 514, 647, 1235
BslI CCNNNNNNNGG 3 cut(s) 228, 670, 2257
BsmAI GTCTC 3 cut(s) 130, 1944, 2457
BsmFI GGGAC 4 cut(s) 257, 514, 647, 1235
BsmI GAATGC 3 cut(s) 1145, 1767, 2316
Bso31I GGTCTC 2 cut(s) 1944, 2457
BsoBI CYCGRG 4 cut(s) 127, 531, 1000, 1171
Bsp119I TTCGAA 2 cut(s) 524, 1586
Bsp1286I GDGCHC 1 cut(s) 648
Bsp1407I TGTACA 1 cut(s) 600
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 3 cut(s) 587, 1187, 1605
BspCNI CTCAG 1 cut(s) 572
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 1 cut(s) 2061
BspLI GGNNCC 5 cut(s) 425, 512, 1080, 1615, 1742
BspMAI CTGCAG 1 cut(s) 1564
BspPI GGATC 6 cut(s) 489, 566, 640, 647, 1496, 1913
BspT104I TTCGAA 2 cut(s) 524, 1586
BspT107I GGYRCC 1 cut(s) 1078
BspTI CTTAAG 1 cut(s) 1144
BspTNI GGTCTC 2 cut(s) 1944, 2457
BsrDI GCAATG 2 cut(s) 32, 1893
BsrFI RCCGGY 3 cut(s) 489, 507, 2183
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 5 cut(s) 1070, 2173, 2457, 2469, 2485
BssAI RCCGGY 3 cut(s) 489, 507, 2183
BssECI CCNNGG 8 cut(s) 163, 372, 541, 617, 953, 1113, 1684, 2256
BssNAI GTATAC 1 cut(s) 2134
BssSI CACGAG 1 cut(s) 1246
BssT1I CCWWGG 3 cut(s) 372, 1113, 2256
Bst1107I GTATAC 1 cut(s) 2134
Bst2BI CACGAG 1 cut(s) 1246
Bst2UI CCWGG 6 cut(s) 165, 542, 954, 1685, 1820, 2427
Bst4CI ACNGT 4 cut(s) 384, 807, 905, 2138
Bst6I CTCTTC 4 cut(s) 1409, 1689, 1773, 2435
BstAFI CTTAAG 1 cut(s) 1144
BstAUI TGTACA 1 cut(s) 600
BstBI TTCGAA 2 cut(s) 524, 1586
BstC8I GCNNGC 5 cut(s) 946, 1187, 1344, 1963, 2339
BstDEI CTNAG 6 cut(s) 559, 1325, 1547, 1667, 1756, 2309
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 5 cut(s) 679, 760, 780, 1208, 1941
BstFNI CGCG 1 cut(s) 2061
BstHHI GCGC 2 cut(s) 148, 2329
BstMAI GTCTC 3 cut(s) 130, 1944, 2457
BstMWI GCNNNNNNNGC 6 cut(s) 145, 490, 823, 837, 1301, 1611
BstNI CCWGG 6 cut(s) 165, 542, 954, 1685, 1820, 2427
BstNSI RCATGY 5 cut(s) 948, 1103, 1807, 2086, 2228
BstSCI CCNGG 8 cut(s) 163, 540, 617, 855, 952, 1683, 1818, 2425
BstSFI CTRYAG 3 cut(s) 1389, 1560, 2517
BstSLI GKGCMC 1 cut(s) 648
BstUI CGCG 1 cut(s) 2061
BstV1I GCAGC 2 cut(s) 1574, 2057
BstX2I RGATCY 4 cut(s) 632, 1501, 1905, 2404
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 4 cut(s) 632, 1501, 1905, 2404
BstZ17I GTATAC 1 cut(s) 2134
Bsu15I ATCGAT 1 cut(s) 282
BsuI GTATCC 2 cut(s) 382, 1225
BsuTUI ATCGAT 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 2114
BtsCI GGATG 5 cut(s) 679, 760, 780, 1208, 1941
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 3 cut(s) 27, 2124, 2464
Cac8I GCNNGC 5 cut(s) 946, 1187, 1344, 1963, 2339
CaiI CAGNNNCTG 1 cut(s) 1505
CfoI GCGC 2 cut(s) 148, 2329
Cfr10I RCCGGY 3 cut(s) 489, 507, 2183
Cfr13I GGNCC 6 cut(s) 336, 807, 854, 890, 1184, 1613
ClaI ATCGAT 1 cut(s) 282
CseI GACGC 1 cut(s) 1881
Csp6I GTAC 7 cut(s) 601, 985, 1216, 1425, 2347, 2472, 2478
CspAI ACCGGT 2 cut(s) 507, 2183
CspCI CAANNNNNGTGG 2 cut(s) 2043, 2078
CviQI GTAC 7 cut(s) 601, 985, 1216, 1425, 2347, 2472, 2478
DdeI CTNAG 6 cut(s) 559, 1325, 1547, 1667, 1756, 2309
DrdI GACNNNNNNGTC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 1116
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 2 cut(s) 375, 1681
Eam1104I CTCTTC 4 cut(s) 1409, 1689, 1773, 2435
Eam1105I GACNNNNNGTC 1 cut(s) 1116
EarI CTCTTC 4 cut(s) 1409, 1689, 1773, 2435
EciI GGCGGA 1 cut(s) 1620
Eco130I CCWWGG 3 cut(s) 372, 1113, 2256
Eco147I AGGCCT 1 cut(s) 297
Eco31I GGTCTC 2 cut(s) 1944, 2457
Eco47I GGWCC 3 cut(s) 336, 807, 890
Eco57I CTGAAG 2 cut(s) 142, 1452
Eco88I CYCGRG 4 cut(s) 127, 531, 1000, 1171
EcoRII CCWGG 6 cut(s) 163, 540, 952, 1683, 1818, 2425
EcoT14I CCWWGG 3 cut(s) 372, 1113, 2256
EcoT22I ATGCAT 1 cut(s) 982
ErhI CCWWGG 3 cut(s) 372, 1113, 2256
FalI AAGNNNNNCTT 4 cut(s) 125, 157, 1127, 1159
FaqI GGGAC 4 cut(s) 257, 514, 647, 1235
FauI CCCGC 1 cut(s) 1194
FauNDI CATATG 1 cut(s) 2074
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 3 cut(s) 1473, 2133, 2268
Fnu4HI GCNGC 2 cut(s) 1563, 2046
FokI GGATG 5 cut(s) 686, 767, 787, 1215, 1928
Fsp4HI GCNGC 2 cut(s) 1563, 2046
GlaI GCGC 2 cut(s) 147, 2328
GluI GCNGC 2 cut(s) 1563, 2046
GsuI CTGGAG 1 cut(s) 2502
HapII CCGG 7 cut(s) 490, 498, 508, 619, 857, 2090, 2184
HgaI GACGC 1 cut(s) 1881
HhaI GCGC 2 cut(s) 148, 2329
Hin6I GCGC 2 cut(s) 146, 2327
HinP1I GCGC 2 cut(s) 146, 2327
HincII GTYRAC 1 cut(s) 2269
HindII GTYRAC 1 cut(s) 2269
HpaII CCGG 7 cut(s) 490, 498, 508, 619, 857, 2090, 2184
HphI GGTGA 4 cut(s) 1059, 2079, 2294, 2406
Hpy166II GTNNAC 6 cut(s) 339, 715, 1105, 1474, 2134, 2269
Hpy188I TCNGA 6 cut(s) 423, 632, 1122, 1528, 2030, 2128
Hpy8I GTNNAC 6 cut(s) 339, 715, 1105, 1474, 2134, 2269
Hpy99I CGWCG 3 cut(s) 119, 704, 2270
HpyCH4III ACNGT 4 cut(s) 384, 807, 905, 2138
HpyCH4IV ACGT 4 cut(s) 117, 181, 702, 735
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 490, 823, 837, 1301, 1611
HpyF3I CTNAG 6 cut(s) 559, 1325, 1547, 1667, 1756, 2309
HpySE526I ACGT 4 cut(s) 117, 181, 702, 735
HspAI GCGC 2 cut(s) 146, 2327
Ksp22I TGATCA 1 cut(s) 24
LmnI GCTCC 5 cut(s) 423, 619, 837, 1025, 2030
Lsp1109I GCAGC 2 cut(s) 1574, 2057
LweI GCATC 7 cut(s) 274, 1037, 1193, 1270, 1776, 2389, 2476
MaeII ACGT 4 cut(s) 117, 181, 702, 735
MfeI CAATTG 1 cut(s) 1284
MflI RGATCY 4 cut(s) 632, 1501, 1905, 2404
MhlI GDGCHC 1 cut(s) 648
MlsI TGGCCA 2 cut(s) 377, 1683
MluNI TGGCCA 2 cut(s) 377, 1683
MlyI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
MmeI TCCRAC 5 cut(s) 366, 401, 1506, 2047, 2200
MnlI CCTC 8 cut(s) 56, 73, 274, 287, 339, 809, 1008, 1895
Mox20I TGGCCA 2 cut(s) 377, 1683
Mph1103I ATGCAT 1 cut(s) 982
MroXI GAANNNNTTC 1 cut(s) 1485
MscI TGGCCA 2 cut(s) 377, 1683
MseI TTAA 7 cut(s) 201, 504, 681, 750, 1145, 1520, 1934
MslI CAYNNNNRTG 1 cut(s) 1033
Msp20I TGGCCA 2 cut(s) 377, 1683
MspCI CTTAAG 1 cut(s) 1144
MspI CCGG 7 cut(s) 490, 498, 508, 619, 857, 2090, 2184
MspR9I CCNGG 8 cut(s) 165, 542, 619, 857, 954, 1685, 1820, 2427
MunI CAATTG 1 cut(s) 1284
Mva1269I GAATGC 3 cut(s) 1145, 1767, 2316
MvaI CCWGG 6 cut(s) 165, 542, 954, 1685, 1820, 2427
MvnI CGCG 1 cut(s) 2061
MwoI GCNNNNNNNGC 6 cut(s) 145, 490, 823, 837, 1301, 1611
NciI CCSGG 2 cut(s) 619, 857
NcoI CCATGG 1 cut(s) 372
NdeI CATATG 1 cut(s) 2074
NlaIV GGNNCC 5 cut(s) 425, 512, 1080, 1615, 1742
NmeAIII GCCGAG 1 cut(s) 2276
NmuCI GTSAC 7 cut(s) 869, 905, 1065, 1207, 1252, 1456, 2085
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 5 cut(s) 948, 1103, 1807, 2086, 2228
NspV TTCGAA 2 cut(s) 524, 1586
PaeI GCATGC 1 cut(s) 948
PaeR7I CTCGAG 3 cut(s) 127, 1000, 1171
PceI AGGCCT 1 cut(s) 297
PciI ACATGT 3 cut(s) 1099, 2082, 2224
PctI GAATGC 3 cut(s) 1145, 1767, 2316
PdmI GAANNNNTTC 1 cut(s) 1485
PflMI CCANNNNNTGG 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 2425
PinAI ACCGGT 2 cut(s) 507, 2183
PkrI GCNGC 2 cut(s) 1564, 2047
PleI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
PpsI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
PscI ACATGT 3 cut(s) 1099, 2082, 2224
PsiI TTATAA 1 cut(s) 1623
Psp1406I AACGTT 1 cut(s) 181
Psp6I CCWGG 6 cut(s) 163, 540, 952, 1683, 1818, 2425
PspGI CCWGG 6 cut(s) 163, 540, 952, 1683, 1818, 2425
PspN4I GGNNCC 5 cut(s) 425, 512, 1080, 1615, 1742
PspPI GGNCC 6 cut(s) 336, 807, 854, 890, 1184, 1613
PspXI VCTCGAGB 2 cut(s) 1000, 1171
PsrI GAACNNNNNNTAC 2 cut(s) 2171, 2203
PstI CTGCAG 1 cut(s) 1564
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 4 cut(s) 632, 1501, 1905, 2404
RsaI GTAC 7 cut(s) 602, 986, 1217, 1426, 2348, 2473, 2479
RsaNI GTAC 7 cut(s) 601, 985, 1216, 1425, 2347, 2472, 2478
RseI CAYNNNNRTG 1 cut(s) 1033
SalI GTCGAC 1 cut(s) 2267
SaqAI TTAA 7 cut(s) 201, 504, 681, 750, 1145, 1520, 1934
SatI GCNGC 2 cut(s) 1563, 2046
Sau96I GGNCC 6 cut(s) 336, 807, 854, 890, 1184, 1613
ScaI AGTACT 2 cut(s) 2473, 2479
SchI GAGTC 5 cut(s) 683, 752, 1079, 1817, 2201
ScrFI CCNGG 8 cut(s) 165, 542, 619, 857, 954, 1685, 1820, 2427
SduI GDGCHC 1 cut(s) 648
SfaNI GCATC 7 cut(s) 274, 1037, 1193, 1270, 1776, 2389, 2476
SfcI CTRYAG 3 cut(s) 1389, 1560, 2517
Sfr274I CTCGAG 3 cut(s) 127, 1000, 1171
SfuI TTCGAA 2 cut(s) 524, 1586
SinI GGWCC 3 cut(s) 336, 807, 890
SlaI CTCGAG 3 cut(s) 127, 1000, 1171
SmiMI CAYNNNNRTG 1 cut(s) 1033
SmlI CTYRAG 6 cut(s) 127, 769, 1000, 1144, 1171, 1257
SmoI CTYRAG 6 cut(s) 127, 769, 1000, 1144, 1171, 1257
SpeI ACTAGT 1 cut(s) 2474
SphI GCATGC 1 cut(s) 948
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 3 cut(s) 587, 1187, 1605
SspI AATATT 2 cut(s) 1969, 2025
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 8 cut(s) 163, 540, 617, 855, 952, 1683, 1818, 2425
StyI CCWWGG 3 cut(s) 372, 1113, 2256
TaaI ACNGT 4 cut(s) 384, 807, 905, 2138
TaiI ACGT 4 cut(s) 120, 184, 705, 738
TatI WGTACW 3 cut(s) 600, 2471, 2477
Tru1I TTAA 7 cut(s) 201, 504, 681, 750, 1145, 1520, 1934
Tru9I TTAA 7 cut(s) 201, 504, 681, 750, 1145, 1520, 1934
TscAI CASTG 3 cut(s) 34, 2124, 2464
TseFI GTSAC 7 cut(s) 869, 905, 1065, 1207, 1252, 1456, 2085
TseI GCWGC 2 cut(s) 1562, 2045
Tsp45I GTSAC 7 cut(s) 869, 905, 1065, 1207, 1252, 1456, 2085
TspGWI ACGGA 2 cut(s) 1059, 1442
TspRI CASTG 3 cut(s) 34, 2124, 2464
Van91I CCANNNNNTGG 1 cut(s) 228
Vha464I CTTAAG 1 cut(s) 1144
VpaK11BI GGWCC 3 cut(s) 336, 807, 890
XapI RAATTY 4 cut(s) 169, 553, 1675, 2212
XceI RCATGY 5 cut(s) 948, 1103, 1807, 2086, 2228
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 3 cut(s) 127, 1000, 1171
XmiI GTMKAC 3 cut(s) 1473, 2133, 2268
XmnI GAANNNNTTC 1 cut(s) 1485
ZrmI AGTACT 2 cut(s) 2473, 2479
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.