Rroxscaffold_2G00123770

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000099
Physical Location & Seq
Reverse (-)
57754140 .. 57756924
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00123770.1

Sequence Viewer

Length: 1962 bp
ATGTTAATACAAGACAAGAAGATAATGGAAGATTGGGTTCCTTCAACTAGACTGGTCTATGATCAATGCATTCCTCATCTTCCTTCCTCGTCTCCCCCTTGCATTTCCACTGATTCCATTACTCCAAACCAAACCATCAAAGACGGCGACGTTATAGTCTCGAACAGGGAGCTCTTTGTTCTTGGGTTCTTCAGCCCGGGAAACTCTGGCAAACGTTACGTTGGAGTTTGGTATAACAAAGTTCCTGAGCAGACCATTGTCTGGGTAGCAAATAGAGACAACCCTGTCAATGATTCCTCTGGTCTTTTAGTGATCGAGGGAGATGGAGGCCTTGTCATATATGGAAAGGACAGAAAAGTCCCTCTTTGGTCTGCTAATGTTACTCTCTCTTCTCCAAAAAATTCCATGGCCAAGCTGTTGGATACAGGTAATTTTGTTTTGCTTGAGAATGGCCGGAGCCTGTGGGAAGGGTTTGATTATCCCTCAAATACAATGCTTCCCTTTATGAAAATTGGGCTGAACCGGCGATCCGGGTTCAACCGTTTCCTAACTTCTTGGAAGTCCCAAGATGACCCGGGAACCGGGAATTGTTCATTTAGGATTGATCCAAATGGGTTTCCGCAGCTGTTTTTGTACAAAGGTCAAGCTCCCCGGTGGCGGGGTGGAGCTTGGATCGGGCACAGATGGAGTGGTGTCCCGAAAATGACTCCTAAATTCATCTTCAACGTTAGTTTTGTGAACAATCAAGATGAGCTGTCCATCATGTATGGTATTACGGACGACTCAATCTTCTCAAGGATGGTGCTTGATGAATCGGGAATCGTCGAACGGTCCACATGGCAAGATCAGGAACACCAATGGATCAAGTTTTGGTCTGCCCCGGTAGAGCTATGTGACAACTATGGCAAGTGTGGTCCAAATAGCAACTGTGACACTTACAATGCTGGTGAGTTCGAGTGTACATGCCTACCTGGGTTTGAACCTAAGTTGCTCCGTCCATGGTACTTGAGAGATGGCTCGGATGGGTGTGTGAGGAAAAAGGAGCATCCATGTGTAAAAATGGAGAGGGGTGCCGCAAGGAGGAATGGATGCGTGACATGGTATGGGGACTTGATGGATACAAGGACTTACACGAATGTCAATCAAGATTTATATGTTCGAGTTGATTCAATTGAATTAGCTAAACACGCAAAGAAGTCTAATGGTTCTCTTAGCAAGAAGGGAAAGCTGGCAATTATTATCACATCTATTTTAGTGTTCTTTCTTGTGATCTCTATCGCATATTGGTCCGTAAAGATGAAGAGAAAAGGTCAGGAAAGACAAAATCTATTTCCATTTGGTGTCATCACACCTTCAATCTACTTGGAAGATTCTCCTAGTAGAACAGATCCTGATGATAGCAGATTAAAGTCGGATCTGCCTTTCTTTGATCTAAGCACCATATCTGCAGCCACAAGAAACTTCTCTGATTCGAACAGGCTTGGAGAAGGCGGATTTGACCATGTTTATAAGGGTGTGCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTAAGCAAAAATTCTGGCCAGGGAAATGAAGAGTTCAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTGAGAATCTTAGGCTATTGCGTTCAAGATGAAGAGAAGATGCTAATCTATGAATACCTGCCTAACAAAAGCCTGGACTCTTTCATTTTCAATGAACAGAAAAGGACATTTCTGGATTGGACAAGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGGATCATGTATCTTCATCAAGATTCGAGATTAAGAATCATCCATAGAGACCTAAAGGCCAGCAATGTTCTACTGGATTATGATATGAACCCCAAACTTGCAGATTTTGGTATGGCTAGAATATTCGGAGCAAACCAAATTGAAGCAAATACAAATCGCGTGGTTGGAACATAG

Protein Analysis

653

Amino Acids

74.17

Weight (kDa)

8.63

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 85 - 187 1.9e-31 D-mannose binding lectin
S_locus_glycop PF00954 219 - 329 2e-29 S-locus glycoprotein domain
Pkinase PF00069 490 - 652 8.6e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 492 - 638 1.8e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1509
AasI GACNNNNNNGTC 3 cut(s) 155, 284, 356
Acc36I ACCTGC 1 cut(s) 1698
AccB1I GGYRCC 1 cut(s) 1070
AccB7I CCANNNNNTGG 1 cut(s) 261
AccII CGCG 1 cut(s) 1947
AciI CCGC 4 cut(s) 620, 658, 1074, 1491
AclI AACGTT 2 cut(s) 214, 726
AclWI GGATC 7 cut(s) 522, 599, 680, 869, 1382, 1422, 1799
AcoI YGGCCR 3 cut(s) 408, 451, 1567
AcsI RAATTY 3 cut(s) 400, 713, 1561
AcuI CTGAAG 1 cut(s) 175
AfaI GTAC 3 cut(s) 635, 961, 1004
AfiI CCNNNNNNNGG 5 cut(s) 261, 581, 657, 658, 1080
AjnI CCWGG 3 cut(s) 970, 1569, 1704
AloI GAACNNNNNNTCC 2 cut(s) 512, 544
AluBI AGCT 9 cut(s) 172, 415, 625, 647, 668, 754, 889, 1181, 1228
AluI AGCT 9 cut(s) 172, 415, 625, 647, 668, 754, 889, 1181, 1228
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 4 cut(s) 96, 163, 270, 1830
AlwI GGATC 7 cut(s) 522, 599, 680, 869, 1382, 1422, 1799
AlwNI CAGNNNCTG 1 cut(s) 1391
Ama87I CYCGRG 2 cut(s) 196, 574
AoxI GGCC 5 cut(s) 328, 408, 451, 1567, 1845
ApeKI GCWGC 2 cut(s) 622, 1448
ApoI RAATTY 3 cut(s) 400, 713, 1561
AspS9I GGNCC 3 cut(s) 831, 914, 1287
AsuC2I CCSGG 8 cut(s) 197, 198, 532, 575, 576, 583, 652, 881
AsuHPI GGTGA 1 cut(s) 959
AsuII TTCGAA 1 cut(s) 1472
AvaI CYCGRG 2 cut(s) 196, 574
AvaII GGWCC 3 cut(s) 831, 914, 1287
BaeGI GKGCMC 1 cut(s) 681
BalI TGGCCA 2 cut(s) 410, 1569
BanI GGYRCC 1 cut(s) 1070
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 634, 1460
BccI CCATC 8 cut(s) 143, 317, 678, 767, 793, 1007, 1016, 1108
BceAI ACGGC 2 cut(s) 160, 1526
BciT130I CCWGG 3 cut(s) 972, 1571, 1706
BciVI GTATCC 2 cut(s) 415, 1111
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 8 cut(s) 197, 198, 532, 575, 576, 583, 652, 881
BcoDI GTCTC 4 cut(s) 96, 163, 270, 1830
BfaI CTAG 5 cut(s) 48, 1377, 1524, 1785, 1905
BfmI CTRYAG 1 cut(s) 1446
BfuAI ACCTGC 1 cut(s) 1698
BfuI GTATCC 2 cut(s) 415, 1111
BisI GCNGC 3 cut(s) 623, 1074, 1449
BlsI GCNGC 3 cut(s) 624, 1075, 1450
Bme18I GGWCC 3 cut(s) 831, 914, 1287
BmeT110I CYCGRG 2 cut(s) 196, 574
BmgT120I GGNCC 3 cut(s) 831, 914, 1287
BmiI GGNNCC 5 cut(s) 39, 458, 580, 1072, 1628
BmsI GCATC 3 cut(s) 1054, 1079, 1662
BoxI GACNNNNGTC 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 1472
BpuEI CTTGAG 3 cut(s) 464, 778, 1027
BpuMI CCSGG 8 cut(s) 197, 198, 532, 575, 576, 583, 652, 881
BsaBI GATNNNNATC 2 cut(s) 1274, 1676
BsaI GGTCTC 1 cut(s) 1830
BsaJI CCNNGG 8 cut(s) 196, 405, 574, 650, 879, 971, 998, 1570
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 5 cut(s) 261, 581, 657, 658, 1080
Bse118I RCCGGY 1 cut(s) 522
Bse1I ACTGG 2 cut(s) 57, 1866
Bse3DI GCAATG 2 cut(s) 1779, 1858
Bse8I GATNNNNATC 2 cut(s) 1274, 1676
BseBI CCWGG 3 cut(s) 972, 1571, 1706
BseDI CCNNGG 8 cut(s) 196, 405, 574, 650, 879, 971, 998, 1570
BseGI GGATG 5 cut(s) 804, 1027, 1045, 1094, 1827
BseJI GATNNNNATC 2 cut(s) 1274, 1676
BseLI CCNNNNNNNGG 5 cut(s) 261, 581, 657, 658, 1080
BseMI GCAATG 2 cut(s) 1779, 1858
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 2 cut(s) 57, 1866
BseSI GKGCMC 1 cut(s) 681
BseXI GCAGC 2 cut(s) 634, 1460
Bsh1236I CGCG 1 cut(s) 1947
BshFI GGCC 5 cut(s) 330, 410, 453, 1569, 1847
BshNI GGYRCC 1 cut(s) 1070
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 2 cut(s) 196, 574
BsiSI CCGG 8 cut(s) 197, 454, 523, 531, 575, 582, 652, 881
BslFI GGGAC 4 cut(s) 344, 547, 680, 1121
BslI CCNNNNNNNGG 5 cut(s) 261, 581, 657, 658, 1080
BsmAI GTCTC 4 cut(s) 96, 163, 270, 1830
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 4 cut(s) 344, 547, 680, 1121
BsmI GAATGC 1 cut(s) 69
BsnI GGCC 5 cut(s) 330, 410, 453, 1569, 1847
Bso31I GGTCTC 1 cut(s) 1830
BsoBI CYCGRG 2 cut(s) 196, 574
Bsp119I TTCGAA 1 cut(s) 1472
Bsp1286I GDGCHC 2 cut(s) 174, 681
Bsp1407I TGTACA 2 cut(s) 633, 959
Bsp19I CCATGG 2 cut(s) 405, 998
BspACI CCGC 4 cut(s) 620, 658, 1074, 1491
BspANI GGCC 5 cut(s) 330, 410, 453, 1569, 1847
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 1947
BspLI GGNNCC 5 cut(s) 39, 458, 580, 1072, 1628
BspMAI CTGCAG 1 cut(s) 1450
BspMI ACCTGC 1 cut(s) 1698
BspPI GGATC 7 cut(s) 522, 599, 680, 869, 1382, 1422, 1799
BspT104I TTCGAA 1 cut(s) 1472
BspT107I GGYRCC 1 cut(s) 1070
BspTNI GGTCTC 1 cut(s) 1830
BsrDI GCAATG 2 cut(s) 1779, 1858
BsrFI RCCGGY 1 cut(s) 522
BsrGI TGTACA 2 cut(s) 633, 959
BsrI ACTGG 2 cut(s) 57, 1866
BssAI RCCGGY 1 cut(s) 522
BssECI CCNNGG 8 cut(s) 196, 405, 574, 650, 879, 971, 998, 1570
BssT1I CCWWGG 2 cut(s) 405, 998
Bst2UI CCWGG 3 cut(s) 972, 1571, 1706
Bst4CI ACNGT 3 cut(s) 542, 831, 929
Bst6I CTCTTC 4 cut(s) 394, 1295, 1575, 1659
BstAUI TGTACA 2 cut(s) 633, 959
BstBI TTCGAA 1 cut(s) 1472
BstC8I GCNNGC 2 cut(s) 1230, 1849
BstDEI CTNAG 6 cut(s) 246, 984, 1211, 1433, 1553, 1642
BstDSI CCRYGG 2 cut(s) 405, 998
BstF5I GGATG 5 cut(s) 804, 1027, 1045, 1094, 1827
BstFNI CGCG 1 cut(s) 1947
BstMAI GTCTC 4 cut(s) 96, 163, 270, 1830
BstMWI GCNNNNNNNGC 2 cut(s) 523, 1187
BstNI CCWGG 3 cut(s) 972, 1571, 1706
BstNSI RCATGY 1 cut(s) 966
BstPAI GACNNNNGTC 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 1446
BstSLI GKGCMC 1 cut(s) 681
BstUI CGCG 1 cut(s) 1947
BstV1I GCAGC 2 cut(s) 634, 1460
BstX2I RGATCY 2 cut(s) 1387, 1414
BstXI CCANNNNNNTGG 1 cut(s) 418
BstYI RGATCY 2 cut(s) 1387, 1414
BsuI GTATCC 2 cut(s) 415, 1111
BsuRI GGCC 5 cut(s) 330, 410, 453, 1569, 1847
BtgI CCRYGG 2 cut(s) 405, 998
BtsCI GGATG 5 cut(s) 804, 1027, 1045, 1094, 1827
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 1698
Cac8I GCNNGC 2 cut(s) 1230, 1849
CaiI CAGNNNCTG 1 cut(s) 1391
Cfr10I RCCGGY 1 cut(s) 522
Cfr13I GGNCC 3 cut(s) 831, 914, 1287
Cfr9I CCCGGG 2 cut(s) 196, 574
CseI GACGC 1 cut(s) 1767
Csp6I GTAC 3 cut(s) 634, 960, 1003
CspCI CAANNNNNGTGG 1 cut(s) 1929
CviAII CATG 9 cut(s) 406, 763, 837, 963, 999, 1050, 1098, 1502, 1795
CviQI GTAC 3 cut(s) 634, 960, 1003
DdeI CTNAG 6 cut(s) 246, 984, 1211, 1433, 1553, 1642
DrdI GACNNNNNNGTC 3 cut(s) 155, 284, 356
DseDI GACNNNNNNGTC 3 cut(s) 155, 284, 356
EaeI YGGCCR 3 cut(s) 408, 451, 1567
Eam1104I CTCTTC 4 cut(s) 394, 1295, 1575, 1659
EarI CTCTTC 4 cut(s) 394, 1295, 1575, 1659
EciI GGCGGA 1 cut(s) 1506
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 405, 998
Eco147I AGGCCT 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 1830
Eco47I GGWCC 3 cut(s) 831, 914, 1287
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 175
Eco88I CYCGRG 2 cut(s) 196, 574
EcoICRI GAGCTC 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 970, 1569, 1704
EcoT14I CCWWGG 2 cut(s) 405, 998
EcoT22I ATGCAT 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 405, 998
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 9 cut(s) 409, 766, 840, 966, 1002, 1053, 1101, 1505, 1798
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 4 cut(s) 344, 547, 680, 1121
FatI CATG 9 cut(s) 405, 762, 836, 962, 998, 1049, 1097, 1501, 1794
FauI CCCGC 1 cut(s) 651
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 3 cut(s) 623, 1074, 1449
FokI GGATG 5 cut(s) 811, 1032, 1034, 1101, 1814
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 3 cut(s) 623, 1074, 1449
FspBI CTAG 5 cut(s) 48, 1377, 1524, 1785, 1905
GluI GCNGC 3 cut(s) 623, 1074, 1449
HaeIII GGCC 5 cut(s) 330, 410, 453, 1569, 1847
HapII CCGG 8 cut(s) 197, 454, 523, 531, 575, 582, 652, 881
HgaI GACGC 1 cut(s) 1767
Hin1II CATG 9 cut(s) 409, 766, 840, 966, 1002, 1053, 1101, 1505, 1798
HpaII CCGG 8 cut(s) 197, 454, 523, 531, 575, 582, 652, 881
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 3 cut(s) 739, 834, 960
Hpy188I TCNGA 4 cut(s) 1021, 1414, 1468, 1916
Hpy8I GTNNAC 3 cut(s) 739, 834, 960
Hpy99I CGWCG 2 cut(s) 152, 827
HpyAV CCTTC 6 cut(s) 51, 93, 461, 1213, 1362, 1481
HpyCH4III ACNGT 3 cut(s) 542, 831, 929
HpyCH4IV ACGT 4 cut(s) 150, 214, 219, 726
HpyCH4V TGCA 5 cut(s) 69, 102, 1448, 1610, 1889
HpyF10VI GCNNNNNNNGC 2 cut(s) 523, 1187
HpyF3I CTNAG 6 cut(s) 246, 984, 1211, 1433, 1553, 1642
HpySE526I ACGT 4 cut(s) 150, 214, 219, 726
Hsp92II CATG 9 cut(s) 409, 766, 840, 966, 1002, 1053, 1101, 1505, 1798
Ksp22I TGATCA 1 cut(s) 61
LmnI GCTCC 7 cut(s) 169, 456, 652, 665, 996, 1042, 1916
Lsp1109I GCAGC 2 cut(s) 634, 1460
LweI GCATC 3 cut(s) 1054, 1079, 1662
MaeI CTAG 5 cut(s) 48, 1377, 1524, 1785, 1905
MaeII ACGT 4 cut(s) 150, 214, 219, 726
MaeIII GTNAC 5 cut(s) 215, 379, 893, 929, 1093
MfeI CAATTG 1 cut(s) 1170
MflI RGATCY 2 cut(s) 1387, 1414
MhlI GDGCHC 2 cut(s) 174, 681
MlsI TGGCCA 2 cut(s) 410, 1569
MluNI TGGCCA 2 cut(s) 410, 1569
MlyI GAGTC 3 cut(s) 700, 776, 1703
MmeI TCCRAC 4 cut(s) 202, 399, 1392, 1933
Mox20I TGGCCA 2 cut(s) 410, 1569
Mph1103I ATGCAT 1 cut(s) 71
MscI TGGCCA 2 cut(s) 410, 1569
MseI TTAA 3 cut(s) 5, 1406, 1820
MslI CAYNNNNRTG 1 cut(s) 1050
Msp20I TGGCCA 2 cut(s) 410, 1569
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 8 cut(s) 197, 454, 523, 531, 575, 582, 652, 881
MunI CAATTG 1 cut(s) 1170
Mva1269I GAATGC 1 cut(s) 69
MvaI CCWGG 3 cut(s) 972, 1571, 1706
MvnI CGCG 1 cut(s) 1947
MwoI GCNNNNNNNGC 2 cut(s) 523, 1187
NciI CCSGG 8 cut(s) 197, 198, 532, 575, 576, 583, 652, 881
NcoI CCATGG 2 cut(s) 405, 998
NlaIII CATG 9 cut(s) 409, 766, 840, 966, 1002, 1053, 1101, 1505, 1798
NlaIV GGNNCC 5 cut(s) 39, 458, 580, 1072, 1628
NmuCI GTSAC 3 cut(s) 893, 929, 1093
NsiI ATGCAT 1 cut(s) 71
NspI RCATGY 1 cut(s) 966
NspV TTCGAA 1 cut(s) 1472
PceI AGGCCT 1 cut(s) 330
PctI GAATGC 1 cut(s) 69
PflMI CCANNNNNTGG 1 cut(s) 261
PkrI GCNGC 3 cut(s) 624, 1075, 1450
PleI GAGTC 3 cut(s) 700, 776, 1703
PpsI GAGTC 3 cut(s) 700, 776, 1703
PshAI GACNNNNGTC 1 cut(s) 257
PsiI TTATAA 1 cut(s) 1509
Psp124BI GAGCTC 1 cut(s) 174
Psp1406I AACGTT 2 cut(s) 214, 726
Psp6I CCWGG 3 cut(s) 970, 1569, 1704
PspGI CCWGG 3 cut(s) 970, 1569, 1704
PspN4I GGNNCC 5 cut(s) 39, 458, 580, 1072, 1628
PspPI GGNCC 3 cut(s) 831, 914, 1287
PstI CTGCAG 1 cut(s) 1450
PstNI CAGNNNCTG 1 cut(s) 1391
PsuI RGATCY 2 cut(s) 1387, 1414
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 3 cut(s) 635, 961, 1004
RsaNI GTAC 3 cut(s) 634, 960, 1003
RseI CAYNNNNRTG 1 cut(s) 1050
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 5, 1406, 1820
SatI GCNGC 3 cut(s) 623, 1074, 1449
Sau96I GGNCC 3 cut(s) 831, 914, 1287
SchI GAGTC 3 cut(s) 700, 776, 1703
SduI GDGCHC 2 cut(s) 174, 681
SfaNI GCATC 3 cut(s) 1054, 1079, 1662
SfcI CTRYAG 1 cut(s) 1446
SfuI TTCGAA 1 cut(s) 1472
SinI GGWCC 3 cut(s) 831, 914, 1287
SmaI CCCGGG 2 cut(s) 198, 576
SmiMI CAYNNNNRTG 1 cut(s) 1050
SmlI CTYRAG 3 cut(s) 443, 793, 1006
SmoI CTYRAG 3 cut(s) 443, 793, 1006
SseBI AGGCCT 1 cut(s) 330
SsiI CCGC 4 cut(s) 620, 658, 1074, 1491
SspI AATATT 1 cut(s) 1911
SspMI CTAG 5 cut(s) 48, 1377, 1524, 1785, 1905
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 1 cut(s) 330
StyI CCWWGG 2 cut(s) 405, 998
TaaI ACNGT 3 cut(s) 542, 831, 929
TaiI ACGT 4 cut(s) 153, 217, 222, 729
TaqI TCGA 7 cut(s) 161, 315, 825, 954, 1159, 1472, 1814
TatI WGTACW 2 cut(s) 633, 959
TauI GCSGC 1 cut(s) 1076
Tru1I TTAA 3 cut(s) 5, 1406, 1820
Tru9I TTAA 3 cut(s) 5, 1406, 1820
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 3 cut(s) 893, 929, 1093
TseI GCWGC 2 cut(s) 622, 1448
Tsp45I GTSAC 3 cut(s) 893, 929, 1093
TspGWI ACGGA 3 cut(s) 791, 983, 1279
TspMI CCCGGG 2 cut(s) 196, 574
TspRI CASTG 1 cut(s) 115
Van91I CCANNNNNTGG 1 cut(s) 261
VpaK11BI GGWCC 3 cut(s) 831, 914, 1287
XapI RAATTY 3 cut(s) 400, 713, 1561
XceI RCATGY 1 cut(s) 966
XmaI CCCGGG 2 cut(s) 196, 574
XspI CTAG 5 cut(s) 48, 1377, 1524, 1785, 1905
Zsp2I ATGCAT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.