MD05G1263100.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000099
Physical Location & Seq
Forward (+)
39870223 .. 39874415
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1263100.v1.1.491

Sequence Viewer

Length: 3147 bp
ATGCATTTTGTGAAAGGTCTGCTAAATGTTTTAATTCTTCAGCTCCTCCTTCTCCAGCTCTGCTCTTCCTTGGACACCATAAAATTTGATCAACCCGTCGGAGACGGTGATTTCTTGGTGTCCAAGAATGGCACCTTTGTCCTGGGATTCTTTAGTCCCAAAATATCGACCAACCGCTATGTCGGAATTTGGTACAAGTTTTCGGAAGACATGGTTGTGTGGGTAGCCAACAGGGATAATCCCATCAATGGCAGCTCAGGAGTTCTCTCAATAGGTTTTGATGGAAACCTAGTGCTGCAAGCCAATACCAGCCAAGGCCTTGTTCCTCTGTGGTCAACAAATGTTTCGATATCATTAGCGAGCAACGATAATATTTTTGCGCAAATTCTTGATTCGGGAAGCCTTGTTTTGGCTAAACAAGGCAGCCAGGATGTTTTTTGGCAGAGCACTGATCACCCTACAAATATTCTTCTTTCGAAAATGAAAATCGGGTTGGACAGAAGAAGTGGCATAAACCGGTTCCTCACGTGTTGGAACTCGGACAATGATCCTGGGATGGGGAACGTTTCATTGAGAATGGACCTGAATGGATCGCCACAGTTGATCTTGTACAAGAATGAGGCTAAGTGGTGGAGGTCAGGACAGTGGGACGGGATTCAATGGGGGGGCATACCTGCTGTGCAGCGGAGCGATGTGTTTAAGATCAATTTTGTGAACAACACAGATGAAGTTGCTGTGCAGTGGACTGTTCTTGACCCTTCGATTTACTCATTGAACAAAATAGACGGGTCGGGAACACTCAACCACCTAGTATGGCAAGAGCAGCAAAACCAGTGGGTTTTACTTTGGTCGGCGCCATTGGATGCCTGTGACAGCTATGGCAAGTGTGGTAAATTCGGCAATTGCAATCCTACTAACATTGGATTCAACTGCACATGTTACCCCGGATATGAACCAATATCACCAAAGGATTGGGATTTGAAAGACGGGAGGGGCGGGTGCAAGAGGCAGCAGGGCTTGCGGTCCATGTGTAGGAACGGCGAGGGGTTTGTGAAGATGGAAAATGTAAAGGTTCCAGACACGTCCTCTATAAAACTGGAGAATAATTTGAGCTTAGAAGCGTGCAAGGAGGAGTGCTTGAAGAACTGCTCATGCTTGGCATATGCAAGTGCAGATGTGAGGAATGGAGGGACTGGATGCATGACATGGTATAGAGATTTGATGGATACTAAGCAGTTCACAGAAGGCGGGCAAAATTTGTATATTCGCGCAGATGCAGTAGTTTTAGCTCAGTATAAAAACAAGTCAGGAGGAGGGTATTTTTCCGGGAGAAGGCGACTGGCTATTATATTGGTAGTGTCAATTTCTGTCGCCTCATTGCTCATCCTCGCAGTTCTATGTTGGTTCAGGAAAAGGAGCAGGAAAGGGAGAGGAGGACAACCCAAATTTCGGAATGATCCCACTGCTTCTGGTTCACGAAGCTATGAAGATTTGCCAATAAAAAATGAGGTTGATGAACACAAAGGAGAGACAGATTTACCTTTTTTCGATTTAACCACTGTGGTTGCAGCCACAGAAAACTTCTCTTTTGCTAACATGCTTGGACATGGCGGCTTCGGCACGGTATATAAGGGATGTCTAGCTGATGGACAGGACATAGCTGTCAAAAGATTATCTAGAAATTCAGGCCAAGGCGTAGATGAATTCAAGAATGAAGTAATGCTTATAGCAAAGCTTCAGCATAGAAATCTTGTGAGACTTTTGGGTTGGTGCATTCATAAAGAAGAGAGGATGCTAATCTATGAATACATGCCGAATCGCAGCTTGGACTTATGCATTTTCGATAAAAACAGAAAGTCATCGTTAGATTGGAGAAAGCGGTTTCAAATTATCATTGGGATTGCTCGAGGCGTCCTATATCTTCATCAAGATTCAAGACTAAAAATAATCCACAGGGATTTGAAGGCAAGCAATGTTTTACTGGATGGTTCGATGAACCCAAAAATATCAGATTTTGGCATGGCAAGAATGTTCGGGGATGATCAAATTGAAGCAAACACGAACAGAGTTGTTGGCACCTACGGTTACATGTCACCAGAGTATGCTATGGATGGGCTATATTCCACAAAATCCGATGTGTTTAGCTTCGGAGTCTTGGCACTAGAGATCATTAGCGGCAGGAAGAACAGTTTCCAATTCGAAAACTCCTCTCTGAATTTGGTTGGAATTATATGGGACTTGTGGACAGAGGGAAAAGTCTTGGACATAGTTGATTCATCACTGAACCAGTCGTATTCGACTCATGAAGTTATGAGATGCATTCAAATTGGGCTCTTGTGCGTGCAAGAATATGCAACAGACCGGCCAACCATGCTAGATGTTGTGTTCATGCTGGGGAATGAAACGAATCTTCCACATCCGAAAAAGGCAGCGTTTAGCTTCAAAAACAGAGGTCCGGACTCTTCAAAGTCTAGAGGAGCTTCCTCTGTGAATGATATAACAGTAACAGTTATTGAAGCTCCGATCAGGGATGGCGAAGTTCTAGTCTCTAGTGGAGAAGTGTTTGAGCTTGGTTTCTTCAGCCCCGGAAAATCCACAAAGCGCTATGTTGGAATCTGGTACATGAAAGATGTAGAAAAAAGAGTTGTATGGGTTGCAAACAGAGACAATCCTGTCAATGACACTTCAGGAGTCCTCTCAATTGGTGCAAATGGAAACCTCTTCCTCTATGCCGAAAACCAAAGCGATATTCCCCTCTGGTCTGCCATATCCAATGTTTCAATCGCATCATTATCATCCAGTTCCGCACATGAGCCTAATTACAAAGCTCAGCTCTTAGATACTGGAAACCTTGTGGTGAAATTTCAACAACAAGATGGCGGCGAAAAGCTAACGGTATGGCAGAGTTTTTATTATCCCAAGCATACAATGCTTCCAAGCATGAAACTTGGACTGGACAGGCTCACGAGATTGAACCGGTTTCTAACTTCTTGGAAGTCCGAAGACGACCCGGGAACTGGAAGTTGCAGCTTCAGAGTAGACCCTAATGGAGCCCCTCAGATGGTCGTGTACAAAGATGATGTTCGATGGTTGCGCTTTGGTAATTTGAACGGTAATAGAGGGTGGCAGAATAGCGCAACCACATAG

Protein Analysis

1049

Amino Acids

117.04

Weight (kDa)

8.19

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 2.9e-28 D-mannose binding lectin
S_locus_glycop PF00954 211 - 319 1.7e-25 S-locus glycoprotein domain
PAN_2 PF08276 344 - 410 9.5e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 530 - 795 5.6e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 531 - 737 3.4e-42 Protein kinase domain
DUF3403 PF11883 800 - 840 9.1e-06 Domain of unknown function (DUF3403)
B_lectin PF01453 882 - 999 2e-29 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1661, 2672
Acc16I TGCGCA 1 cut(s) 381
Acc36I ACCTGC 1 cut(s) 682
AccB1I GGYRCC 3 cut(s) 131, 853, 2075
AccI GTMKAC 1 cut(s) 3039
AccII CGCG 1 cut(s) 1269
AccIII TCCGGA 1 cut(s) 2455
AclI AACGTT 1 cut(s) 564
AclWI GGATC 3 cut(s) 542, 598, 1451
AcoI YGGCCR 1 cut(s) 2363
AcuI CTGAAG 5 cut(s) 23, 1721, 2563, 2670, 3016
AcvI CACGTG 1 cut(s) 528
AcyI GRCGYC 2 cut(s) 854, 1911
AfaI GTAC 4 cut(s) 194, 611, 2621, 3071
AfeI AGCGCT 1 cut(s) 2603
AfiI CCNNNNNNNGG 8 cut(s) 248, 409, 557, 1032, 1332, 1449, 3017, 3061
AflIII ACRYGT 4 cut(s) 527, 935, 1080, 2088
AgeI ACCGGT 2 cut(s) 516, 2976
AjiI CACGTC 1 cut(s) 1083
AjnI CCWGG 3 cut(s) 141, 426, 550
AjuI GAANNNNNNNTTGG 8 cut(s) 306, 338, 476, 508, 1808, 1840, 2733, 2765
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 5 cut(s) 96, 1523, 1750, 2551, 2658
AlwI GGATC 3 cut(s) 542, 598, 1451
Ama87I CYCGRG 2 cut(s) 1905, 3010
Aor13HI TCCGGA 1 cut(s) 2455
Aor51HI AGCGCT 1 cut(s) 2603
AoxI GGCC 3 cut(s) 316, 1687, 2363
ArsI GACNNNNNNTTYG 2 cut(s) 2529, 2561
AsiGI ACCGGT 2 cut(s) 516, 2976
Asp700I GAANNNNTTC 1 cut(s) 2189
AspLEI GCGC 6 cut(s) 382, 856, 1271, 2604, 3096, 3137
AspS9I GGNCC 3 cut(s) 580, 1023, 2453
AsuC2I CCSGG 5 cut(s) 945, 1327, 2586, 3011, 3012
AsuHPI GGTGA 5 cut(s) 119, 446, 954, 2085, 2869
AsuII TTCGAA 2 cut(s) 476, 2199
AvaI CYCGRG 2 cut(s) 1905, 3010
AvaII GGWCC 3 cut(s) 580, 1023, 2453
BanI GGYRCC 3 cut(s) 131, 853, 2075
BanII GRGCYC 2 cut(s) 2334, 3055
BauI CACGAG 1 cut(s) 2965
BbrPI CACGTG 1 cut(s) 528
BbsI GAAGAC 2 cut(s) 213, 3009
Bbv12I GWGCWC 1 cut(s) 449
BceAI ACGGC 1 cut(s) 1054
BciT130I CCWGG 3 cut(s) 143, 428, 552
BciVI GTATCC 1 cut(s) 1219
BclI TGATCA 3 cut(s) 88, 451, 2041
BcnI CCSGG 5 cut(s) 945, 1327, 2586, 3011, 3012
BcoDI GTCTC 5 cut(s) 96, 1523, 1750, 2551, 2658
BfaI CTAG 9 cut(s) 290, 809, 1640, 1677, 2162, 2375, 2472, 2543, 2550
BfoI RGCGCY 2 cut(s) 857, 2605
BfuAI ACCTGC 1 cut(s) 682
BfuI GTATCC 1 cut(s) 1219
BlpI GCTNAGC 1 cut(s) 2829
Bme1390I CCNGG 8 cut(s) 143, 428, 552, 945, 1327, 2586, 3011, 3012
Bme18I GGWCC 3 cut(s) 580, 1023, 2453
BmeT110I CYCGRG 2 cut(s) 1905, 3010
BmgBI CACGTC 1 cut(s) 1083
BmgT120I GGNCC 3 cut(s) 580, 1023, 2453
BmiI GGNNCC 6 cut(s) 133, 521, 855, 1074, 2077, 3052
BmrFI CCNGG 8 cut(s) 143, 428, 552, 945, 1327, 2586, 3011, 3012
BmsI GCATC 6 cut(s) 853, 1187, 1264, 1782, 2306, 2795
BpiI GAAGAC 2 cut(s) 213, 3009
BpmI CTGGAG 2 cut(s) 38, 1118
Bpu10I CCTNAGC 1 cut(s) 256
Bpu1102I GCTNAGC 1 cut(s) 2829
Bpu14I TTCGAA 2 cut(s) 476, 2199
BpuMI CCSGG 5 cut(s) 945, 1327, 2586, 3011, 3012
BsaAI YACGTR 1 cut(s) 528
BsaBI GATNNNNATC 1 cut(s) 1796
BsaHI GRCGYC 2 cut(s) 854, 1911
BsaJI CCNNGG 8 cut(s) 69, 142, 313, 551, 943, 1690, 2584, 3010
BsaWI WCCGGW 3 cut(s) 516, 2455, 2976
Bsc4I CCNNNNNNNGG 8 cut(s) 248, 409, 557, 1032, 1332, 1449, 3017, 3061
Bse118I RCCGGY 3 cut(s) 516, 2361, 2976
Bse3DI GCAATG 2 cut(s) 1376, 1978
Bse8I GATNNNNATC 1 cut(s) 1796
BseAI TCCGGA 1 cut(s) 2455
BseBI CCWGG 3 cut(s) 143, 428, 552
BseDI CCNNGG 8 cut(s) 69, 142, 313, 551, 943, 1690, 2584, 3010
BseJI GATNNNNATC 1 cut(s) 1796
BseLI CCNNNNNNNGG 8 cut(s) 248, 409, 557, 1032, 1332, 1449, 3017, 3061
BseMI GCAATG 2 cut(s) 1376, 1978
BseMII CTCAG 4 cut(s) 270, 1304, 2843, 3071
BseRI GAGGAG 6 cut(s) 35, 1145, 1326, 1446, 2197, 2490
BseYI CCCAGC 1 cut(s) 2392
BsgI GTGCAG 4 cut(s) 701, 758, 916, 1191
Bsh1236I CGCG 1 cut(s) 1269
BshFI GGCC 3 cut(s) 318, 1689, 2365
BshNI GGYRCC 3 cut(s) 131, 853, 2075
BshTI ACCGGT 2 cut(s) 516, 2976
BsiHKAI GWGCWC 1 cut(s) 449
BsiHKCI CYCGRG 2 cut(s) 1905, 3010
BsiSI CCGG 8 cut(s) 517, 945, 1326, 2362, 2456, 2586, 2977, 3011
BslFI GGGAC 4 cut(s) 141, 662, 1204, 2249
BslI CCNNNNNNNGG 8 cut(s) 248, 409, 557, 1032, 1332, 1449, 3017, 3061
BsmAI GTCTC 5 cut(s) 96, 1523, 1750, 2551, 2658
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 4 cut(s) 141, 662, 1204, 2249
BsmI GAATGC 2 cut(s) 1773, 2319
BsnI GGCC 3 cut(s) 318, 1689, 2365
BsoBI CYCGRG 2 cut(s) 1905, 3010
Bsp119I TTCGAA 2 cut(s) 476, 2199
Bsp1286I GDGCHC 3 cut(s) 449, 2334, 3055
Bsp13I TCCGGA 1 cut(s) 2455
Bsp1407I TGTACA 2 cut(s) 609, 3069
Bsp1720I GCTNAGC 1 cut(s) 2829
BspANI GGCC 3 cut(s) 318, 1689, 2365
BspCNI CTCAG 4 cut(s) 269, 1303, 2842, 3070
BspEI TCCGGA 1 cut(s) 2455
BspFNI CGCG 1 cut(s) 1269
BspHI TCATGA 1 cut(s) 2302
BspLI GGNNCC 6 cut(s) 133, 521, 855, 1074, 2077, 3052
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 3 cut(s) 542, 598, 1451
BspQI GCTCTTC 1 cut(s) 70
BspT104I TTCGAA 2 cut(s) 476, 2199
BspT107I GGYRCC 3 cut(s) 131, 853, 2075
BsrDI GCAATG 2 cut(s) 1376, 1978
BsrFI RCCGGY 3 cut(s) 516, 2361, 2976
BsrGI TGTACA 2 cut(s) 609, 3069
BssAI RCCGGY 3 cut(s) 516, 2361, 2976
BssECI CCNNGG 8 cut(s) 69, 142, 313, 551, 943, 1690, 2584, 3010
BssNI GRCGYC 2 cut(s) 854, 1911
BssSI CACGAG 1 cut(s) 2965
BssT1I CCWWGG 3 cut(s) 69, 313, 1690
Bst2BI CACGAG 1 cut(s) 2965
Bst2UI CCWGG 3 cut(s) 143, 428, 552
Bst6I CTCTTC 4 cut(s) 70, 1779, 2467, 2726
BstACI GRCGYC 2 cut(s) 854, 1911
BstAPI GCANNNNNTGC 2 cut(s) 1018, 2929
BstAUI TGTACA 2 cut(s) 609, 3069
BstBAI YACGTR 1 cut(s) 528
BstBI TTCGAA 2 cut(s) 476, 2199
BstC8I GCNNGC 7 cut(s) 300, 361, 1019, 1123, 1250, 1969, 2342
BstDEI CTNAG 8 cut(s) 256, 624, 1114, 1230, 1290, 2829, 2836, 3057
BstFNI CGCG 1 cut(s) 1269
BstH2I RGCGCY 2 cut(s) 857, 2605
BstHHI GCGC 6 cut(s) 382, 856, 1271, 2604, 3096, 3137
BstMAI GTCTC 5 cut(s) 96, 1523, 1750, 2551, 2658
BstMWI GCNNNNNNNGC 5 cut(s) 823, 1018, 1617, 2371, 2929
BstNI CCWGG 3 cut(s) 143, 428, 552
BstNSI RCATGY 4 cut(s) 939, 1600, 1813, 2092
BstSCI CCNGG 8 cut(s) 141, 426, 550, 943, 1325, 2584, 3009, 3010
BstUI CGCG 1 cut(s) 1269
BstV2I GAAGAC 2 cut(s) 213, 3009
BstXI CCANNNNNNTGG 1 cut(s) 972
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 3 cut(s) 318, 1689, 2365
BtgZI GCGATG 1 cut(s) 705
BtrI CACGTC 1 cut(s) 1083
BtsI GCAGTG 2 cut(s) 746, 1461
BtsIMutI CAGTG 7 cut(s) 447, 650, 746, 839, 1461, 1557, 2279
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 7 cut(s) 300, 361, 1019, 1123, 1250, 1969, 2342
CciI TCATGA 1 cut(s) 2302
CfoI GCGC 6 cut(s) 382, 856, 1271, 2604, 3096, 3137
Cfr10I RCCGGY 3 cut(s) 516, 2361, 2976
Cfr13I GGNCC 3 cut(s) 580, 1023, 2453
Cfr9I CCCGGG 1 cut(s) 3010
CseI GACGC 1 cut(s) 1900
Csp6I GTAC 4 cut(s) 193, 610, 2620, 3070
CspAI ACCGGT 2 cut(s) 516, 2976
CspCI CAANNNNNGTGG 4 cut(s) 815, 850, 1546, 1581
CviQI GTAC 4 cut(s) 193, 610, 2620, 3070
DdeI CTNAG 8 cut(s) 256, 624, 1114, 1230, 1290, 2829, 2836, 3057
DinI GGCGCC 1 cut(s) 855
DrdI GACNNNNNNGTC 2 cut(s) 1661, 2672
DseDI GACNNNNNNGTC 2 cut(s) 1661, 2672
EaeI YGGCCR 1 cut(s) 2363
Eam1104I CTCTTC 4 cut(s) 70, 1779, 2467, 2726
EarI CTCTTC 4 cut(s) 70, 1779, 2467, 2726
Eco130I CCWWGG 3 cut(s) 69, 313, 1690
Eco147I AGGCCT 1 cut(s) 318
Eco24I GRGCYC 2 cut(s) 2334, 3055
Eco32I GATATC 1 cut(s) 351
Eco47I GGWCC 3 cut(s) 580, 1023, 2453
Eco47III AGCGCT 1 cut(s) 2603
Eco57I CTGAAG 5 cut(s) 23, 1721, 2563, 2670, 3016
Eco72I CACGTG 1 cut(s) 528
Eco88I CYCGRG 2 cut(s) 1905, 3010
EcoRI GAATTC 1 cut(s) 1703
EcoRII CCWGG 3 cut(s) 141, 426, 550
EcoRV GATATC 1 cut(s) 351
EcoT14I CCWWGG 3 cut(s) 69, 313, 1690
EcoT22I ATGCAT 4 cut(s) 6, 1202, 1838, 2321
EcoT38I GRGCYC 2 cut(s) 2334, 3055
EgeI GGCGCC 1 cut(s) 855
EheI GGCGCC 1 cut(s) 855
ErhI CCWWGG 3 cut(s) 69, 313, 1690
Esp3I CGTCTC 1 cut(s) 96
FalI AAGNNNNNCTT 2 cut(s) 1707, 1739
FaqI GGGAC 4 cut(s) 141, 662, 1204, 2249
FauI CCCGC 2 cut(s) 989, 1241
FauNDI CATATG 1 cut(s) 1162
FbaI TGATCA 3 cut(s) 88, 451, 2041
FblI GTMKAC 1 cut(s) 3039
FriOI GRGCYC 2 cut(s) 2334, 3055
FspBI CTAG 9 cut(s) 290, 809, 1640, 1677, 2162, 2375, 2472, 2543, 2550
FspI TGCGCA 1 cut(s) 381
GlaI GCGC 6 cut(s) 381, 855, 1270, 2603, 3095, 3136
GsaI CCCAGC 1 cut(s) 2396
GsuI CTGGAG 2 cut(s) 38, 1118
HaeII RGCGCY 2 cut(s) 857, 2605
HaeIII GGCC 3 cut(s) 318, 1689, 2365
HapII CCGG 8 cut(s) 517, 945, 1326, 2362, 2456, 2586, 2977, 3011
HgaI GACGC 1 cut(s) 1900
HhaI GCGC 6 cut(s) 382, 856, 1271, 2604, 3096, 3137
Hin1I GRCGYC 2 cut(s) 854, 1911
Hin6I GCGC 6 cut(s) 380, 854, 1269, 2602, 3094, 3135
HinP1I GCGC 6 cut(s) 380, 854, 1269, 2602, 3094, 3135
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 1733
HpaII CCGG 8 cut(s) 517, 945, 1326, 2362, 2456, 2586, 2977, 3011
HphI GGTGA 5 cut(s) 119, 446, 954, 2085, 2869
Hpy166II GTNNAC 8 cut(s) 336, 715, 744, 1239, 1475, 2244, 3040, 3070
Hpy8I GTNNAC 8 cut(s) 336, 715, 744, 1239, 1475, 2244, 3040, 3070
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 5 cut(s) 59, 768, 1238, 1326, 1957
HpyCH4IV ACGT 3 cut(s) 527, 564, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 823, 1018, 1617, 2371, 2929
HpyF3I CTNAG 8 cut(s) 256, 624, 1114, 1230, 1290, 2829, 2836, 3057
HpySE526I ACGT 3 cut(s) 527, 564, 1082
Hsp92I GRCGYC 2 cut(s) 854, 1911
HspAI GCGC 6 cut(s) 380, 854, 1269, 2602, 3094, 3135
KasI GGCGCC 1 cut(s) 853
Kpn2I TCCGGA 1 cut(s) 2455
Ksp22I TGATCA 3 cut(s) 88, 451, 2041
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 6 cut(s) 48, 687, 1416, 2477, 2524, 3050
LweI GCATC 6 cut(s) 853, 1187, 1264, 1782, 2306, 2795
MaeI CTAG 9 cut(s) 290, 809, 1640, 1677, 2162, 2375, 2472, 2543, 2550
MaeII ACGT 3 cut(s) 527, 564, 1082
MaeIII GTNAC 5 cut(s) 869, 938, 2084, 2091, 2503
MfeI CAATTG 2 cut(s) 901, 2700
MhlI GDGCHC 3 cut(s) 449, 2334, 3055
Mly113I GGCGCC 1 cut(s) 854
MlyI GAGTC 4 cut(s) 2160, 2293, 2453, 2700
MmeI TCCRAC 6 cut(s) 79, 163, 474, 512, 2203, 2590
Mph1103I ATGCAT 4 cut(s) 6, 1202, 1838, 2321
MroI TCCGGA 1 cut(s) 2455
MroXI GAANNNNTTC 1 cut(s) 2189
MseI TTAA 3 cut(s) 32, 699, 1553
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 8 cut(s) 517, 945, 1326, 2362, 2456, 2586, 2977, 3011
MspR9I CCNGG 8 cut(s) 143, 428, 552, 945, 1327, 2586, 3011, 3012
MunI CAATTG 2 cut(s) 901, 2700
Mva1269I GAATGC 2 cut(s) 1773, 2319
MvaI CCWGG 3 cut(s) 143, 428, 552
MvnI CGCG 1 cut(s) 1269
MwoI GCNNNNNNNGC 5 cut(s) 823, 1018, 1617, 2371, 2929
NarI GGCGCC 1 cut(s) 854
NciI CCSGG 5 cut(s) 945, 1327, 2586, 3011, 3012
NdeI CATATG 1 cut(s) 1162
NlaIV GGNNCC 6 cut(s) 133, 521, 855, 1074, 2077, 3052
NmuCI GTSAC 2 cut(s) 869, 2091
NsbI TGCGCA 1 cut(s) 381
NsiI ATGCAT 4 cut(s) 6, 1202, 1838, 2321
NspI RCATGY 4 cut(s) 939, 1600, 1813, 2092
NspV TTCGAA 2 cut(s) 476, 2199
PaeR7I CTCGAG 1 cut(s) 1905
PagI TCATGA 1 cut(s) 2302
PceI AGGCCT 1 cut(s) 318
PciI ACATGT 2 cut(s) 935, 2088
PciSI GCTCTTC 1 cut(s) 70
PctI GAATGC 2 cut(s) 1773, 2319
PdmI GAANNNNTTC 1 cut(s) 2189
PfeI GAWTC 9 cut(s) 147, 392, 655, 924, 1816, 1931, 2273, 2407, 2613
PfoI TCCNGGA 1 cut(s) 1325
PinAI ACCGGT 2 cut(s) 516, 2976
PleI GAGTC 4 cut(s) 2159, 2293, 2453, 2699
PluTI GGCGCC 1 cut(s) 857
PmaCI CACGTG 1 cut(s) 528
PmlI CACGTG 1 cut(s) 528
PpsI GAGTC 4 cut(s) 2159, 2293, 2453, 2699
Ppu21I YACGTR 1 cut(s) 528
PscI ACATGT 2 cut(s) 935, 2088
Psp1406I AACGTT 1 cut(s) 564
Psp6I CCWGG 3 cut(s) 141, 426, 550
PspCI CACGTG 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 2392
PspGI CCWGG 3 cut(s) 141, 426, 550
PspN4I GGNNCC 6 cut(s) 133, 521, 855, 1074, 2077, 3052
PspPI GGNCC 3 cut(s) 580, 1023, 2453
PspXI VCTCGAGB 1 cut(s) 1905
RsaI GTAC 4 cut(s) 194, 611, 2621, 3071
RsaNI GTAC 4 cut(s) 193, 610, 2620, 3070
RseI CAYNNNNRTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 3 cut(s) 32, 699, 1553
Sau96I GGNCC 3 cut(s) 580, 1023, 2453
SchI GAGTC 4 cut(s) 2160, 2293, 2453, 2700
ScrFI CCNGG 8 cut(s) 143, 428, 552, 945, 1327, 2586, 3011, 3012
SduI GDGCHC 3 cut(s) 449, 2334, 3055
SfaNI GCATC 6 cut(s) 853, 1187, 1264, 1782, 2306, 2795
SfoI GGCGCC 1 cut(s) 855
Sfr274I CTCGAG 1 cut(s) 1905
SfuI TTCGAA 2 cut(s) 476, 2199
SinI GGWCC 3 cut(s) 580, 1023, 2453
SlaI CTCGAG 1 cut(s) 1905
SmaI CCCGGG 1 cut(s) 3012
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 1905
SmoI CTYRAG 1 cut(s) 1905
SseBI AGGCCT 1 cut(s) 318
SspDI GGCGCC 1 cut(s) 853
SspI AATATT 2 cut(s) 373, 466
SspMI CTAG 9 cut(s) 290, 809, 1640, 1677, 2162, 2375, 2472, 2543, 2550
StuI AGGCCT 1 cut(s) 318
StyD4I CCNGG 8 cut(s) 141, 426, 550, 943, 1325, 2584, 3009, 3010
StyI CCWWGG 3 cut(s) 69, 313, 1690
TaiI ACGT 3 cut(s) 530, 567, 1085
TatI WGTACW 2 cut(s) 609, 3069
TauI GCSGC 3 cut(s) 1614, 2178, 2883
TfiI GAWTC 9 cut(s) 147, 392, 655, 924, 1816, 1931, 2273, 2407, 2613
Tru1I TTAA 3 cut(s) 32, 699, 1553
Tru9I TTAA 3 cut(s) 32, 699, 1553
TscAI CASTG 7 cut(s) 454, 650, 746, 839, 1468, 1564, 2286
TseFI GTSAC 2 cut(s) 869, 2091
Tsp45I GTSAC 2 cut(s) 869, 2091
TspMI CCCGGG 1 cut(s) 3010
TspRI CASTG 7 cut(s) 454, 650, 746, 839, 1468, 1564, 2286
VpaK11BI GGWCC 3 cut(s) 580, 1023, 2453
XbaI TCTAGA 2 cut(s) 1676, 2471
XceI RCATGY 4 cut(s) 939, 1600, 1813, 2092
XhoI CTCGAG 1 cut(s) 1905
XmaI CCCGGG 1 cut(s) 3010
XmiI GTMKAC 1 cut(s) 3039
XmnI GAANNNNTTC 1 cut(s) 2189
XspI CTAG 9 cut(s) 290, 809, 1640, 1677, 2162, 2375, 2472, 2543, 2550
Zsp2I ATGCAT 4 cut(s) 6, 1202, 1838, 2321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.