Rw5G022540

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000099
Physical Location & Seq
Reverse (-)
31290390 .. 31294658
4269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022540.1

Sequence Viewer

Length: 2529 bp
ATGAGTTCCAGTATCAAAGCGTTTATCAAGATATTACTTCTCTTCCTTCTTCTCCCCTCTAGCATTTGCCAGCTTTCCCTTGACGCCCTTAAGCCAAACCGTCCCATCAGAGATGGTGACGTTTTAGTCTCTAGCACGCAAATCTTTGCACTAGGGTTCTTCAGCCCTGGAAATCCTCAGCACCGCTATCAAACCATTGTCTGGGTTGCGAACAGAGACAACCCAGTCAATGATTCCTATGGAGTGCTATCGATCAATGGTGATGGAGGCCTTGTCATACATGGAAAGGACCCAAGTACCCCTCTTTGGTCCGCAAACGTTACTCTCTCTTCACCATACAATTTTATAGCCAAACTTTTGGATACAGGAAATCTTGTTTTACTTGAGAATGGTAGCAAAAAAGTTGTGTGGGAAGGCTTCGATTACCCCTCAGATACATTGCTTTCCTTTATGAAGATAGGGCTGAACCGGCGGTCCGGGTTGGAGTGGCACCTAACATCTTGGAAGTCAAAAGATGACCCTGGAACCGGGAGTTGTACATATGGGATAGACCCTATGGGGTTTCCGCAGTTTTTTCTGCGCAAAGGTAGAGAAGCATTGTGGCGGACCGGCCCTTGGATCGGGGACAAATTGAGCGCTCTACCTATAATGACGGCTACCTTCTACAATGCAAGTTTTGTCAACAATGAAGACGAGATATCCTTAGTGTTTGCTATTGCTAAGGACTCATTGATCACAAGGGTGGTGATAGATGAATCAGGAATAGTTCAAGTGTTCGTGTGGAATGATCAATGGATCAAATTCTACTCCCACCCGACTGAGTGGTGTGATTACTATGGACAGTGCGGTCCAAATACTAACTGTGACTCAGAGAAGGACTATAAGTTGGCTTGCACGTGCCTACCTGGGTTCGAATCCAAATCACCTAGTTTGAGTGTTGGTGAGAGTGGATGCATTAGGAAAGCAGGAGCATCCACATGTCAAAATGGAGAAGGGTTCGTGAAGGTGGCGCGTGTAAAAATACCGGACTCATCTACGGCATATGTGAACATGAGTATGAGTTTGGAAGAGTGCAAACAAAATTGCTTGATGGATTGTTCTTGCACAGCTTACACGAGTGCGGATGATCGGGGAGGTGGGATTGGGTGTGTGACATGGCACGGGGACTTGATGGACATGAGGACTTTCTCCGATGTTGGTCAAGATTTATATGTTCGAGTCGATGTAACTTCTTTAGGTACGTCTTTGGATTTTTTTTTTCCGGTACTTTGGATTTTGGGTATTTACTTATTTCATACCTTCGTAGCTCAATATGCAAAGTCAGGTGGTTCTCTTAGCAAGAAGGCAAGGCTGGCAATTTCACTTGGATCTCGTAGGCAAAATAAATATTCATTTGAACTTACTTTTTTGTGTTTGATAATAGGTAAGCGTAGGCAAAATAAATATTCATTTGAACTTACTGCTCGGTCAACCTACTTCGAAGAAGCTACTGGTGGATTAGTTCTTGATGATAGTAGAATAAACTCGGAGTTACTATTATTTCATCTAAACACCATAGCCAACGCCACAAACAATTTCTCCATTGAAAACAAGCTTGGAGAAGGAGGGTTTGGCCCCGTTTATAAGGGGATACTTTACGATGGAAAAGAAATAGCCGTAAAAAGACTATCCAAGTTTTCTGGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTGATTGCAAAACTCCAACATAGAAACCTTGTTAAGATTTTAGGTTGTTGTTTTGAAGGTGAAGAGAAGATTCTAATCTACGAATACTTGCCAAACAAAAGTTTGGACTCTTTCATCTTTAATGAAACAAGGAGAGCACTTTTAAGTTGGACAAGACGCTTCGAGATTATCTTGGGGATTGCTAGAGGCTTATTATATCTTCATGAAGATTCAAGACTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTTCTATTGGATAATTCTTTGAACCCAAAGATTGCAGATTTTGGTATGGCTAGAATATTTAGAGGGGAGCAAACTGAAGCAAATACAAAACGTGTGGTTGGAACATATGGTTATATGTCACCGGAATATGCAATGGAAGGACTGTTTTCAATAAAGTCTGACGTGTATAGTTTTGGTGTTTTATTGCTAGAAATCATTACTGGCAAAAAGAATGCTGGTTCCTATGAGAAGTATCCATATTCAAATTTGATTGGACATGTTTGGAAGTTGTGGAAAGAAGGTAGAGCTGTGGAAATCATTGATTCATCTATAGGTGAATCTTACCTTGTTAGTGAAATAATAAGGTGCATTCAAATCGCACTCCTGTGTGTGCAAGAATTTGCGACTGACCGGCCAACCATGTCGGTAGTTGTTTCAATGTTAAGTAATGATGCAGCTCTTCCTTCACCGAGGCGACCCGCATTTTTATTGAAGACAATGAGTCCTAGTGGAGACTCATACAACAACAAAGGAGGTTGTTCAGTAAATGATGTCACATGTACAATTGTAGAAGCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

842

Amino Acids

93.82

Weight (kDa)

7.1

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 65 - 169 1.4e-32 D-mannose binding lectin
S_locus_glycop PF00954 201 - 307 1e-22 S-locus glycoprotein domain
PAN_2 PF08276 327 - 393 1.1e-22 PAN-like domain
PK_Tyr_Ser-Thr PF07714 527 - 795 4.1e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 527 - 729 5.1e-42 Protein kinase domain
DUF3403 PF11883 798 - 842 3.5e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1623
AasI GACNNNNNNGTC 3 cut(s) 125, 224, 846
Acc16I TGCGCA 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 489
AccB7I CCANNNNNTGG 1 cut(s) 201
AccII CGCG 1 cut(s) 1012
AciI CCGC 8 cut(s) 184, 312, 472, 566, 604, 846, 1121, 2427
AclI AACGTT 1 cut(s) 318
AclWI GGATC 3 cut(s) 626, 803, 1375
AcoI YGGCCR 2 cut(s) 1681, 2360
AcsI RAATTY 3 cut(s) 800, 2212, 2345
AcuI CTGAAG 2 cut(s) 145, 2064
AcvI CACGTG 1 cut(s) 897
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 5 cut(s) 298, 538, 1240, 1266, 2509
AfeI AGCGCT 1 cut(s) 637
AfiI CCNNNNNNNGG 5 cut(s) 201, 306, 527, 615, 620
AflII CTTAAG 1 cut(s) 89
AflIII ACRYGT 5 cut(s) 977, 2059, 2130, 2224, 2504
AhdI GACNNNNNGTC 1 cut(s) 2448
AjiI CACGTC 1 cut(s) 2131
AjnI CCWGG 3 cut(s) 166, 520, 904
AjuI GAANNNNNNNTTGG 4 cut(s) 1578, 1593, 1610, 1625
AleI CACNNNNGTG 2 cut(s) 740, 2332
AloI GAACNNNNNNTCC 2 cut(s) 458, 490
AluBI AGCT 8 cut(s) 73, 1109, 1307, 1487, 1594, 2255, 2405, 2522
AluI AGCT 8 cut(s) 73, 1109, 1307, 1487, 1594, 2255, 2405, 2522
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 3 cut(s) 133, 210, 2454
AlwI GGATC 3 cut(s) 626, 803, 1375
Aor51HI AGCGCT 1 cut(s) 637
AoxI GGCC 6 cut(s) 268, 610, 1612, 1681, 1959, 2360
ApeKI GCWGC 1 cut(s) 2402
ApoI RAATTY 3 cut(s) 800, 2212, 2345
Asp700I GAANNNNTTC 1 cut(s) 1713
AspLEI GCGC 3 cut(s) 582, 638, 1012
AspS9I GGNCC 7 cut(s) 289, 309, 474, 606, 611, 848, 1613
AsuC2I CCSGG 2 cut(s) 478, 529
AsuII TTCGAA 2 cut(s) 912, 1479
AvaII GGWCC 5 cut(s) 289, 309, 474, 606, 848
BaeI ACNNNNGTAYC 2 cut(s) 280, 313
BalI TGGCCA 1 cut(s) 1683
BanI GGYRCC 1 cut(s) 489
BauI CACGAG 1 cut(s) 1114
BbrPI CACGTG 1 cut(s) 897
BbsI GAAGAC 2 cut(s) 696, 2447
Bbv12I GWGCWC 1 cut(s) 1855
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 1 cut(s) 2414
BccI CCATC 6 cut(s) 107, 113, 257, 1084, 1165, 1634
BceAI ACGGC 3 cut(s) 669, 1053, 1640
BcgI CGANNNNNNTGC 2 cut(s) 2305, 2339
BciT130I CCWGG 3 cut(s) 168, 522, 906
BciVI GTATCC 3 cut(s) 355, 1623, 2211
BclI TGATCA 2 cut(s) 732, 787
BcnI CCSGG 2 cut(s) 478, 529
BcoDI GTCTC 3 cut(s) 133, 210, 2454
BfaI CTAG 8 cut(s) 60, 132, 152, 927, 1899, 2019, 2156, 2454
BfmI CTRYAG 1 cut(s) 2277
BfoI RGCGCY 1 cut(s) 639
BfrI CTTAAG 1 cut(s) 89
BfuI GTATCC 3 cut(s) 355, 1623, 2211
BglII AGATCT 1 cut(s) 1951
BisI GCNGC 1 cut(s) 2403
BlsI GCNGC 1 cut(s) 2404
Bme1390I CCNGG 5 cut(s) 168, 478, 522, 529, 906
Bme18I GGWCC 5 cut(s) 289, 309, 474, 606, 848
BmeRI GACNNNNNGTC 1 cut(s) 2448
BmgBI CACGTC 1 cut(s) 2131
BmgT120I GGNCC 7 cut(s) 289, 309, 474, 606, 611, 848, 1613
BmiI GGNNCC 5 cut(s) 291, 491, 526, 1615, 2188
BmrFI CCNGG 5 cut(s) 168, 478, 522, 529, 906
BmrI ACTGGG 1 cut(s) 218
BmsI GCATC 3 cut(s) 941, 980, 2389
BmuI ACTGGG 1 cut(s) 218
BpiI GAAGAC 2 cut(s) 696, 2447
Bpu10I CCTNAGC 2 cut(s) 177, 720
Bpu14I TTCGAA 2 cut(s) 912, 1479
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 2 cut(s) 478, 529
Bsa29I ATCGAT 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 897
BsaBI GATNNNNATC 1 cut(s) 1790
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 6 cut(s) 166, 520, 614, 905, 1684, 2417
BsaWI WCCGGW 3 cut(s) 1024, 1261, 2089
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 1562, 1592
Bsc4I CCNNNNNNNGG 5 cut(s) 201, 306, 527, 615, 620
Bse118I RCCGGY 3 cut(s) 468, 608, 2358
Bse1I ACTGG 4 cut(s) 9, 224, 1495, 2173
Bse3DI GCAATG 3 cut(s) 437, 1972, 2106
Bse8I GATNNNNATC 1 cut(s) 1790
BseBI CCWGG 3 cut(s) 168, 522, 906
BseCI ATCGAT 1 cut(s) 251
BseDI CCNNGG 6 cut(s) 166, 520, 614, 905, 1684, 2417
BseGI GGATG 3 cut(s) 956, 971, 1129
BseJI GATNNNNATC 1 cut(s) 1790
BseLI CCNNNNNNNGG 5 cut(s) 201, 306, 527, 615, 620
BseMI GCAATG 3 cut(s) 437, 1972, 2106
BseMII CTCAG 4 cut(s) 191, 444, 810, 882
BseNI ACTGG 4 cut(s) 9, 224, 1495, 2173
BseXI GCAGC 1 cut(s) 2414
Bsh1236I CGCG 1 cut(s) 1012
BshFI GGCC 6 cut(s) 270, 612, 1614, 1683, 1961, 2362
BshNI GGYRCC 1 cut(s) 489
BshVI ATCGAT 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 1855
BsiSI CCGG 8 cut(s) 469, 477, 528, 609, 1025, 1262, 2090, 2359
BslFI GGGAC 3 cut(s) 87, 638, 1178
BslI CCNNNNNNNGG 5 cut(s) 201, 306, 527, 615, 620
BsmAI GTCTC 3 cut(s) 133, 210, 2454
BsmFI GGGAC 3 cut(s) 87, 638, 1178
BsmI GAATGC 2 cut(s) 2185, 2316
BsnI GGCC 6 cut(s) 270, 612, 1614, 1683, 1961, 2362
Bsp119I TTCGAA 2 cut(s) 912, 1479
Bsp1286I GDGCHC 1 cut(s) 1855
Bsp1407I TGTACA 2 cut(s) 536, 2507
Bsp143I GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
BspACI CCGC 8 cut(s) 184, 312, 472, 566, 604, 846, 1121, 2427
BspANI GGCC 6 cut(s) 270, 612, 1614, 1683, 1961, 2362
BspCNI CTCAG 4 cut(s) 190, 443, 811, 881
BspDI ATCGAT 1 cut(s) 251
BspFNI CGCG 1 cut(s) 1012
BspHI TCATGA 1 cut(s) 1918
BspLI GGNNCC 5 cut(s) 291, 491, 526, 1615, 2188
BspPI GGATC 3 cut(s) 626, 803, 1375
BspQI GCTCTTC 1 cut(s) 2412
BspT104I TTCGAA 2 cut(s) 912, 1479
BspT107I GGYRCC 1 cut(s) 489
BspTI CTTAAG 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 437, 1972, 2106
BsrFI RCCGGY 3 cut(s) 468, 608, 2358
BsrGI TGTACA 2 cut(s) 536, 2507
BsrI ACTGG 4 cut(s) 9, 224, 1495, 2173
BssAI RCCGGY 3 cut(s) 468, 608, 2358
BssECI CCNNGG 6 cut(s) 166, 520, 614, 905, 1684, 2417
BssMI GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 1 cut(s) 1114
BssT1I CCWWGG 2 cut(s) 614, 1684
Bst2BI CACGAG 1 cut(s) 1114
Bst2UI CCWGG 3 cut(s) 168, 522, 906
Bst4CI ACNGT 4 cut(s) 101, 843, 863, 2112
Bst6I CTCTTC 6 cut(s) 47, 334, 1062, 1689, 1773, 2412
BstACI GRCGYC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 89
BstAUI TGTACA 2 cut(s) 536, 2507
BstBAI YACGTR 1 cut(s) 897
BstBI TTCGAA 2 cut(s) 912, 1479
BstC8I GCNNGC 6 cut(s) 71, 137, 892, 1353, 1963, 2524
BstDEI CTNAG 8 cut(s) 177, 430, 703, 720, 819, 868, 1334, 1934
BstF5I GGATG 3 cut(s) 956, 971, 1129
BstFNI CGCG 1 cut(s) 1012
BstH2I RGCGCY 1 cut(s) 639
BstHHI GCGC 3 cut(s) 582, 638, 1012
BstKTI GATC 8 cut(s) 255, 621, 735, 790, 798, 1129, 1370, 1954
BstMAI GTCTC 3 cut(s) 133, 210, 2454
BstMBI GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
BstMWI GCNNNNNNNGC 3 cut(s) 469, 1313, 1352
BstNI CCWGG 3 cut(s) 168, 522, 906
BstNSI RCATGY 3 cut(s) 981, 2228, 2508
BstSCI CCNGG 5 cut(s) 166, 476, 520, 527, 904
BstSFI CTRYAG 1 cut(s) 2277
BstUI CGCG 1 cut(s) 1012
BstV1I GCAGC 1 cut(s) 2414
BstV2I GAAGAC 2 cut(s) 696, 2447
BstX2I RGATCY 2 cut(s) 1367, 1951
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 2 cut(s) 1367, 1951
Bsu15I ATCGAT 1 cut(s) 251
BsuI GTATCC 3 cut(s) 355, 1623, 2211
BsuRI GGCC 6 cut(s) 270, 612, 1614, 1683, 1961, 2362
BsuTUI ATCGAT 1 cut(s) 251
BtrI CACGTC 1 cut(s) 2131
BtsCI GGATG 3 cut(s) 956, 971, 1129
BtsIMutI CAGTG 1 cut(s) 848
Cac8I GCNNGC 6 cut(s) 71, 137, 892, 1353, 1963, 2524
CciI TCATGA 1 cut(s) 1918
CfoI GCGC 3 cut(s) 582, 638, 1012
Cfr10I RCCGGY 3 cut(s) 468, 608, 2358
Cfr13I GGNCC 7 cut(s) 289, 309, 474, 606, 611, 848, 1613
ClaI ATCGAT 1 cut(s) 251
CpoI CGGWCCG 2 cut(s) 474, 606
CseI GACGC 2 cut(s) 92, 1881
Csp6I GTAC 5 cut(s) 297, 537, 1239, 1265, 2508
CspCI CAANNNNNGTGG 2 cut(s) 2043, 2078
CspI CGGWCCG 2 cut(s) 474, 606
CviAII CATG 9 cut(s) 281, 978, 1051, 1155, 1177, 1919, 2225, 2368, 2505
CviQI GTAC 5 cut(s) 297, 537, 1239, 1265, 2508
DdeI CTNAG 8 cut(s) 177, 430, 703, 720, 819, 868, 1334, 1934
DpnI GATC 8 cut(s) 254, 620, 734, 789, 797, 1128, 1369, 1953
DpnII GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
DrdI GACNNNNNNGTC 3 cut(s) 125, 224, 846
DriI GACNNNNNGTC 1 cut(s) 2448
DseDI GACNNNNNNGTC 3 cut(s) 125, 224, 846
EaeI YGGCCR 2 cut(s) 1681, 2360
Eam1104I CTCTTC 6 cut(s) 47, 334, 1062, 1689, 1773, 2412
Eam1105I GACNNNNNGTC 1 cut(s) 2448
EarI CTCTTC 6 cut(s) 47, 334, 1062, 1689, 1773, 2412
EciI GGCGGA 1 cut(s) 619
Eco130I CCWWGG 2 cut(s) 614, 1684
Eco147I AGGCCT 1 cut(s) 270
Eco32I GATATC 1 cut(s) 699
Eco47I GGWCC 5 cut(s) 289, 309, 474, 606, 848
Eco47III AGCGCT 1 cut(s) 637
Eco57I CTGAAG 2 cut(s) 145, 2064
Eco72I CACGTG 1 cut(s) 897
EcoO109I RGGNCCY 1 cut(s) 289
EcoRII CCWGG 3 cut(s) 166, 520, 904
EcoRV GATATC 1 cut(s) 699
EcoT14I CCWWGG 2 cut(s) 614, 1684
EcoT22I ATGCAT 1 cut(s) 956
ErhI CCWWGG 2 cut(s) 614, 1684
FaeI CATG 9 cut(s) 284, 981, 1054, 1158, 1180, 1922, 2228, 2371, 2508
FaqI GGGAC 3 cut(s) 87, 638, 1178
FatI CATG 9 cut(s) 280, 977, 1050, 1154, 1176, 1918, 2224, 2367, 2504
FauI CCCGC 1 cut(s) 2434
FauNDI CATATG 3 cut(s) 541, 1042, 2074
FbaI TGATCA 2 cut(s) 732, 787
Fnu4HI GCNGC 1 cut(s) 2403
FokI GGATG 3 cut(s) 958, 963, 1136
Fsp4HI GCNGC 1 cut(s) 2403
FspBI CTAG 8 cut(s) 60, 132, 152, 927, 1899, 2019, 2156, 2454
FspI TGCGCA 1 cut(s) 581
GlaI GCGC 3 cut(s) 581, 637, 1011
GluI GCNGC 1 cut(s) 2403
HaeII RGCGCY 1 cut(s) 639
HaeIII GGCC 6 cut(s) 270, 612, 1614, 1683, 1961, 2362
HapII CCGG 8 cut(s) 469, 477, 528, 609, 1025, 1262, 2090, 2359
HgaI GACGC 2 cut(s) 92, 1881
HhaI GCGC 3 cut(s) 582, 638, 1012
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 9 cut(s) 284, 981, 1054, 1158, 1180, 1922, 2228, 2371, 2508
Hin6I GCGC 3 cut(s) 580, 636, 1010
HinP1I GCGC 3 cut(s) 580, 636, 1010
HincII GTYRAC 2 cut(s) 682, 1470
HindII GTYRAC 2 cut(s) 682, 1470
HindIII AAGCTT 1 cut(s) 1592
HpaII CCGG 8 cut(s) 469, 477, 528, 609, 1025, 1262, 2090, 2359
Hpy166II GTNNAC 3 cut(s) 682, 1048, 1470
Hpy188I TCNGA 7 cut(s) 110, 433, 871, 1192, 1528, 1719, 2128
Hpy188III TCNNGA 8 cut(s) 28, 759, 1000, 1202, 1505, 1879, 1919, 1929
Hpy8I GTNNAC 3 cut(s) 682, 1048, 1470
HpyCH4III ACNGT 4 cut(s) 101, 843, 863, 2112
HpyCH4IV ACGT 6 cut(s) 120, 318, 896, 1241, 2059, 2130
HpyF10VI GCNNNNNNNGC 3 cut(s) 469, 1313, 1352
HpyF3I CTNAG 8 cut(s) 177, 430, 703, 720, 819, 868, 1334, 1934
HpySE526I ACGT 6 cut(s) 120, 318, 896, 1241, 2059, 2130
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 9 cut(s) 284, 981, 1054, 1158, 1180, 1922, 2228, 2371, 2508
HspAI GCGC 3 cut(s) 580, 636, 1010
Ksp22I TGATCA 2 cut(s) 732, 787
Kzo9I GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
LguI GCTCTTC 1 cut(s) 2412
LmnI GCTCC 2 cut(s) 968, 2035
Lsp1109I GCAGC 1 cut(s) 2414
LweI GCATC 3 cut(s) 941, 980, 2389
MaeI CTAG 8 cut(s) 60, 132, 152, 927, 1899, 2019, 2156, 2454
MaeII ACGT 6 cut(s) 120, 318, 896, 1241, 2059, 2130
MaeIII GTNAC 8 cut(s) 116, 319, 863, 1150, 1225, 1530, 2085, 2500
MalI GATC 8 cut(s) 254, 620, 734, 789, 797, 1128, 1369, 1953
MboI GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
MfeI CAATTG 1 cut(s) 2511
MflI RGATCY 2 cut(s) 1367, 1951
MhlI GDGCHC 1 cut(s) 1855
MlsI TGGCCA 1 cut(s) 1683
MluNI TGGCCA 1 cut(s) 1683
MlyI GAGTC 7 cut(s) 719, 860, 1022, 1227, 1817, 2456, 2458
MmeI TCCRAC 4 cut(s) 462, 1756, 1844, 2047
Mox20I TGGCCA 1 cut(s) 1683
Mph1103I ATGCAT 1 cut(s) 956
MroXI GAANNNNTTC 1 cut(s) 1713
MscI TGGCCA 1 cut(s) 1683
MseI TTAA 6 cut(s) 90, 1701, 1749, 1836, 1859, 2390
MslI CAYNNNNRTG 4 cut(s) 740, 976, 1055, 2332
Msp20I TGGCCA 1 cut(s) 1683
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 8 cut(s) 469, 477, 528, 609, 1025, 1262, 2090, 2359
MspR9I CCNGG 5 cut(s) 168, 478, 522, 529, 906
MunI CAATTG 1 cut(s) 2511
Mva1269I GAATGC 2 cut(s) 2185, 2316
MvaI CCWGG 3 cut(s) 168, 522, 906
MvnI CGCG 1 cut(s) 1012
MwoI GCNNNNNNNGC 3 cut(s) 469, 1313, 1352
NciI CCSGG 2 cut(s) 478, 529
NdeI CATATG 3 cut(s) 541, 1042, 2074
NdeII GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
NlaIII CATG 9 cut(s) 284, 981, 1054, 1158, 1180, 1922, 2228, 2371, 2508
NlaIV GGNNCC 5 cut(s) 291, 491, 526, 1615, 2188
NmuCI GTSAC 5 cut(s) 116, 863, 1150, 2085, 2500
NsbI TGCGCA 1 cut(s) 581
NsiI ATGCAT 1 cut(s) 956
NspI RCATGY 3 cut(s) 981, 2228, 2508
NspV TTCGAA 2 cut(s) 912, 1479
OliI CACNNNNGTG 2 cut(s) 740, 2332
PagI TCATGA 1 cut(s) 1918
PceI AGGCCT 1 cut(s) 270
PciI ACATGT 3 cut(s) 977, 2224, 2504
PciSI GCTCTTC 1 cut(s) 2412
PctI GAATGC 2 cut(s) 2185, 2316
PdmI GAANNNNTTC 1 cut(s) 1713
PfeI GAWTC 7 cut(s) 233, 755, 914, 1786, 1925, 2270, 2285
PflMI CCANNNNNTGG 1 cut(s) 201
PkrI GCNGC 1 cut(s) 2404
PleI GAGTC 7 cut(s) 719, 860, 1022, 1226, 1817, 2456, 2457
PmaCI CACGTG 1 cut(s) 897
PmlI CACGTG 1 cut(s) 897
PpsI GAGTC 7 cut(s) 719, 860, 1022, 1226, 1817, 2456, 2457
Ppu21I YACGTR 1 cut(s) 897
PpuMI RGGWCCY 1 cut(s) 289
PscI ACATGT 3 cut(s) 977, 2224, 2504
PsiI TTATAA 1 cut(s) 1623
Psp1406I AACGTT 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 3 cut(s) 166, 520, 904
PspCI CACGTG 1 cut(s) 897
PspGI CCWGG 3 cut(s) 166, 520, 904
PspN4I GGNNCC 5 cut(s) 291, 491, 526, 1615, 2188
PspPI GGNCC 7 cut(s) 289, 309, 474, 606, 611, 848, 1613
PspPPI RGGWCCY 1 cut(s) 289
PsuI RGATCY 2 cut(s) 1367, 1951
RsaI GTAC 5 cut(s) 298, 538, 1240, 1266, 2509
RsaNI GTAC 5 cut(s) 297, 537, 1239, 1265, 2508
RseI CAYNNNNRTG 4 cut(s) 740, 976, 1055, 2332
Rsr2I CGGWCCG 2 cut(s) 474, 606
RsrII CGGWCCG 2 cut(s) 474, 606
SapI GCTCTTC 1 cut(s) 2412
SaqAI TTAA 6 cut(s) 90, 1701, 1749, 1836, 1859, 2390
SatI GCNGC 1 cut(s) 2403
Sau3AI GATC 8 cut(s) 252, 618, 732, 787, 795, 1126, 1367, 1951
Sau96I GGNCC 7 cut(s) 289, 309, 474, 606, 611, 848, 1613
SchI GAGTC 7 cut(s) 719, 860, 1022, 1227, 1817, 2456, 2458
ScrFI CCNGG 5 cut(s) 168, 478, 522, 529, 906
SduI GDGCHC 1 cut(s) 1855
SfaNI GCATC 3 cut(s) 941, 980, 2389
SfcI CTRYAG 1 cut(s) 2277
SfuI TTCGAA 2 cut(s) 912, 1479
SinI GGWCC 5 cut(s) 289, 309, 474, 606, 848
SmiMI CAYNNNNRTG 4 cut(s) 740, 976, 1055, 2332
SmlI CTYRAG 2 cut(s) 89, 383
SmoI CTYRAG 2 cut(s) 89, 383
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 8 cut(s) 184, 312, 472, 566, 604, 846, 1121, 2427
SspI AATATT 3 cut(s) 1388, 1445, 2025
SspMI CTAG 8 cut(s) 60, 132, 152, 927, 1899, 2019, 2156, 2454
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 5 cut(s) 166, 476, 520, 527, 904
StyI CCWWGG 2 cut(s) 614, 1684
TaaI ACNGT 4 cut(s) 101, 843, 863, 2112
TaiI ACGT 6 cut(s) 123, 321, 899, 1244, 2062, 2133
TaqI TCGA 7 cut(s) 251, 420, 912, 1216, 1221, 1479, 1878
TaqII GACCGA 1 cut(s) 1455
TatI WGTACW 2 cut(s) 536, 2507
TfiI GAWTC 7 cut(s) 233, 755, 914, 1786, 1925, 2270, 2285
Tru1I TTAA 6 cut(s) 90, 1701, 1749, 1836, 1859, 2390
Tru9I TTAA 6 cut(s) 90, 1701, 1749, 1836, 1859, 2390
TscAI CASTG 1 cut(s) 848
TseFI GTSAC 5 cut(s) 116, 863, 1150, 2085, 2500
TseI GCWGC 1 cut(s) 2402
Tsp45I GTSAC 5 cut(s) 116, 863, 1150, 2085, 2500
TspRI CASTG 1 cut(s) 848
Van91I CCANNNNNTGG 1 cut(s) 201
Vha464I CTTAAG 1 cut(s) 89
VpaK11BI GGWCC 5 cut(s) 289, 309, 474, 606, 848
XapI RAATTY 3 cut(s) 800, 2212, 2345
XceI RCATGY 3 cut(s) 981, 2228, 2508
XmnI GAANNNNTTC 1 cut(s) 1713
XspI CTAG 8 cut(s) 60, 132, 152, 927, 1899, 2019, 2156, 2454
Zsp2I ATGCAT 1 cut(s) 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.