Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000099

Physical Location & Seq

Genomic Coordinates Forward (+)

45624528 .. 45626404

Gene Structure 1877 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1332700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGAGAAAGCTAACAATGAATTCTACTAAATTCTTTTTTGTCAGTACTGTATTGTTCCTCTTCCTTGTGATTCTTCCCTCTTCCATTTCGGTTACCCTAGACGCCATTACACCAAACCAACCCCTAAGAGAGGGCGACGTTCTTCTCTCCACCACTGAATTCTTTGCACTAGGGTTTTTTAAGCCAGGCAATTCTCATAACCGTTACATCGGAGTTTGGTACAACAAAATTCCAATCAAAACCATTGTCTGGATTGCAAACAGAGAAAACCCCATAGTTCCTACTGCTGGAACTGGACTTCTAGCCATTCATGCAGATCATGGTGGCCTTGTTATGTATGGGGAGGACCAAAATACCCCTTTCTGGTCCACTAATCTCACCGTCTCTTCTCCAAACAATTCCGTGATAGCCAAGCTTTGGGATACGGGAAATCTTGTTGTAGTTGAAATTAATGGCGGTAGTAGTGAAAAGGTGTTGTGGCAAGGTTTTGATTATCCCACAAATACGATGCTTCCTTTTATGAAACTTGGGCTGGACAGGCGATCCGGGTTGAACTGGTTCCTCACATCTTGGAAGTCCCAAGATGACCCGGGAATGGGCAACTGCTCGTACCGGATAGAGACAAGTGAGAGTCCACAGGGGTTCTTATACATGGGTCAGACTCCATTGTGGCGGACTGGACCTTGGATCGGTGAAAGATGGTCCGGGGTGCCTCAAATTAAAAAAACTTTCTACTCCAACGTTACTTTTGTGAACACTCAAGATGAGGTATCCATCATGGATGGTACTGTTTATGAATCAATCTTCTTTAAAATGGTGATCAATGAATCAGGAAACATTGAACGGTCCGCATGGCATGATAATAAATGGGTCCCGTATTGGTCGCCGGATCGGCAGTGTGATTTTTACGGGAAGTGTGGTCCGAATAGCATTTGTTACCCAAACCCAAACTTTGCGGATAAATTTGAGTGCAAATGCCTACCTGGATTCGAACCCAAGTTGCAACATGAGTGGTATTTGGGAGATTGGTCAGGCGGGTGCGTGAGGCAAAAAGGATCATCCGTTTGCCAAAACGGGGAAGGGTTCTTGAAGTTGGAAAGTGTGAAGGTGCCGGACACTTCTACGGCACGTGTGAACATGAGTAGGAGTGGGGAAGCATGTAAAGAAGAATGCCTGAGAAATTGTTCTTGCACGGCATACGCAAATGCAGATGAAAGGCAGGGAGGGAGTGGCTGTATAACATGGCATGGCGACTTGATTGACACGAGGACCTTTTTGTATACGGGTCAAGATTTGTATGTGCGAGTTGATGCAAATGTTTCAGCTCAATACGCAAAGAAGTCAAACAGTTCTTTTGGCAAGAAGAGGAAGCTGGAAGTTTCAGTAGCATGTGGTCTATTGTTCTTCCTTTTGTTTTCCCTTGCATGTTGGTTGGTAAAGAGGAAGAGAAAAGGTAAACAAATTTCCTTTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

492

Amino Acids

55.36

Weight (kDa)

8.5

Isoelectric Point (pI)

43.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	81 - 192	7.1e-30	D-mannose binding lectin
S_locus_glycop	PF00954	224 - 333	1.4e-21	S-locus glycoprotein domain
PAN_2	PF08276	356 - 422	8.4e-23	PAN-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000099)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca	FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica	MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica	Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis	pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis	RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata	RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora	Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii	Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa	Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha	Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana	Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	709, 1108
AccB7I	CCANNNNNTGG	2 cut(s)	249, 417
AccI	GTMKAC	1 cut(s)	1280
AciI	CCGC	5 cut(s)	456, 673, 849, 956, 1035
AclI	AACGTT	1 cut(s)	741
AclWI	GGATC	4 cut(s)	537, 695, 897, 1063
AcsI	RAATTY	6 cut(s)	19, 29, 158, 228, 962, 1461
AcvI	CACGTG	1 cut(s)	1130
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	4 cut(s)	46, 221, 611, 787
AfiI	CCNNNNNNNGG	9 cut(s)	130, 249, 287, 363, 417, 595, 596, 689, 1075
AflIII	ACRYGT	1 cut(s)	1129
AgsI	TTSAA	4 cut(s)	446, 553, 842, 1090
AjnI	CCWGG	2 cut(s)	184, 982
AluBI	AGCT	4 cut(s)	10, 415, 1325, 1372
AluI	AGCT	4 cut(s)	10, 415, 1325, 1372
Alw26I	GTCTC	2 cut(s)	388, 614
AlwI	GGATC	4 cut(s)	537, 695, 897, 1063
Ama87I	CYCGRG	1 cut(s)	589
AoxI	GGCC	1 cut(s)	325
ApoI	RAATTY	6 cut(s)	19, 29, 158, 228, 962, 1461
ArsI	GACNNNNNNTTYG	2 cut(s)	231, 263
AseI	ATTAAT	1 cut(s)	450
Asp700I	GAANNNNTTC	2 cut(s)	557, 1183
AspS9I	GGNCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
AsuC2I	CCSGG	4 cut(s)	547, 590, 591, 706
AsuHPI	GGTGA	3 cut(s)	370, 704, 829
AsuII	TTCGAA	1 cut(s)	990
AvaI	CYCGRG	1 cut(s)	589
AvaII	GGWCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
BanI	GGYRCC	2 cut(s)	709, 1108
BauI	CACGAG	1 cut(s)	1264
BbrPI	CACGTG	1 cut(s)	1130
BccI	CCATC	3 cut(s)	693, 776, 782
BceAI	ACGGC	2 cut(s)	1140, 1209
BciT130I	CCWGG	2 cut(s)	186, 984
BciVI	GTATCC	2 cut(s)	415, 781
BclI	TGATCA	1 cut(s)	819
BcnI	CCSGG	4 cut(s)	547, 590, 591, 706
BcoDI	GTCTC	2 cut(s)	388, 614
BfaI	CTAG	4 cut(s)	98, 170, 302, 1474
BfuI	GTATCC	2 cut(s)	415, 781
BglI	GCCNNNNNGGC	1 cut(s)	892
BmcAI	AGTACT	1 cut(s)	46
Bme1390I	CCNGG	6 cut(s)	186, 547, 590, 591, 706, 984
Bme18I	GGWCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
BmeT110I	CYCGRG	1 cut(s)	589
BmgT120I	GGNCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
BmiI	GGNNCC	5 cut(s)	560, 711, 872, 873, 1110
BmrFI	CCNGG	6 cut(s)	186, 547, 590, 591, 706, 984
BmsI	GCATC	2 cut(s)	498, 1300
Bpu14I	TTCGAA	1 cut(s)	990
BpuEI	CTTGAG	1 cut(s)	744
BpuMI	CCSGG	4 cut(s)	547, 590, 591, 706
BsaAI	YACGTR	1 cut(s)	1130
BsaHI	GRCGYC	1 cut(s)	102
BsaJI	CCNNGG	3 cut(s)	589, 683, 705
BsaWI	WCCGGW	1 cut(s)	612
BsaXI	ACNNNNNCTCC	2 cut(s)	1219, 1249
Bsc4I	CCNNNNNNNGG	9 cut(s)	130, 249, 287, 363, 417, 595, 596, 689, 1075
Bse1I	ACTGG	3 cut(s)	298, 560, 682
BseBI	CCWGG	2 cut(s)	186, 984
BseDI	CCNNGG	3 cut(s)	589, 683, 705
BseGI	GGATG	2 cut(s)	787, 1058
BseLI	CCNNNNNNNGG	9 cut(s)	130, 249, 287, 363, 417, 595, 596, 689, 1075
BseMII	CTCAG	1 cut(s)	1166
BseNI	ACTGG	3 cut(s)	298, 560, 682
BshFI	GGCC	1 cut(s)	327
BshNI	GGYRCC	2 cut(s)	709, 1108
BsiHKCI	CYCGRG	1 cut(s)	589
BsiSI	CCGG	6 cut(s)	546, 590, 613, 705, 887, 1112
BslFI	GGGAC	2 cut(s)	562, 857
BslI	CCNNNNNNNGG	9 cut(s)	130, 249, 287, 363, 417, 595, 596, 689, 1075
BsmAI	GTCTC	2 cut(s)	388, 614
BsmBI	CGTCTC	1 cut(s)	388
BsmFI	GGGAC	2 cut(s)	562, 857
BsmI	GAATGC	1 cut(s)	1175
BsnI	GGCC	1 cut(s)	327
BsoBI	CYCGRG	1 cut(s)	589
Bsp119I	TTCGAA	1 cut(s)	990
Bsp143I	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
BspACI	CCGC	5 cut(s)	456, 673, 849, 956, 1035
BspANI	GGCC	1 cut(s)	327
BspCNI	CTCAG	1 cut(s)	1167
BspLI	GGNNCC	5 cut(s)	560, 711, 872, 873, 1110
BspPI	GGATC	4 cut(s)	537, 695, 897, 1063
BspT104I	TTCGAA	1 cut(s)	990
BspT107I	GGYRCC	2 cut(s)	709, 1108
BsrI	ACTGG	3 cut(s)	298, 560, 682
BssECI	CCNNGG	3 cut(s)	589, 683, 705
BssMI	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
BssNAI	GTATAC	1 cut(s)	1281
BssNI	GRCGYC	1 cut(s)	102
BssSI	CACGAG	1 cut(s)	1264
BssT1I	CCWWGG	1 cut(s)	683
Bst1107I	GTATAC	1 cut(s)	1281
Bst2BI	CACGAG	1 cut(s)	1264
Bst2UI	CCWGG	2 cut(s)	186, 984
Bst4CI	ACNGT	6 cut(s)	49, 203, 382, 790, 846, 1349
Bst6I	CTCTTC	5 cut(s)	65, 85, 391, 1358, 1439
BstACI	GRCGYC	1 cut(s)	102
BstBAI	YACGTR	1 cut(s)	1130
BstBI	TTCGAA	1 cut(s)	990
BstDEI	CTNAG	2 cut(s)	125, 1175
BstEII	GGTNACC	1 cut(s)	91
BstENI	CCTNNNNNAGG	1 cut(s)	128
BstF5I	GGATG	2 cut(s)	787, 1058
BstKTI	GATC	6 cut(s)	319, 545, 690, 822, 892, 1058
BstMAI	GTCTC	2 cut(s)	388, 614
BstMBI	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
BstMWI	GCNNNNNNNGC	4 cut(s)	311, 538, 892, 1331
BstNI	CCWGG	2 cut(s)	186, 984
BstNSI	RCATGY	3 cut(s)	1161, 1392, 1428
BstPI	GGTNACC	1 cut(s)	91
BstSCI	CCNGG	6 cut(s)	184, 545, 588, 589, 704, 982
BstZ17I	GTATAC	1 cut(s)	1281
BsuI	GTATCC	2 cut(s)	415, 781
BsuRI	GGCC	1 cut(s)	327
BtsCI	GGATG	2 cut(s)	787, 1058
BtsI	GCAGTG	1 cut(s)	902
BtsIMutI	CAGTG	2 cut(s)	153, 902
Cfr13I	GGNCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
Cfr9I	CCCGGG	1 cut(s)	589
CpoI	CGGWCCG	1 cut(s)	846
CseI	GACGC	1 cut(s)	110
Csp6I	GTAC	4 cut(s)	45, 220, 610, 786
CspCI	CAANNNNNGTGG	2 cut(s)	992, 1027
CspI	CGGWCCG	1 cut(s)	846
CviQI	GTAC	4 cut(s)	45, 220, 610, 786
DdeI	CTNAG	2 cut(s)	125, 1175
DpnI	GATC	6 cut(s)	318, 544, 689, 821, 891, 1057
DpnII	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
DraI	TTTAAA	1 cut(s)	811
Eam1104I	CTCTTC	5 cut(s)	65, 85, 391, 1358, 1439
EarI	CTCTTC	5 cut(s)	65, 85, 391, 1358, 1439
EciI	GGCGGA	1 cut(s)	688
Eco130I	CCWWGG	1 cut(s)	683
Eco47I	GGWCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
Eco72I	CACGTG	1 cut(s)	1130
Eco88I	CYCGRG	1 cut(s)	589
Eco91I	GGTNACC	1 cut(s)	91
EcoNI	CCTNNNNNAGG	1 cut(s)	128
EcoO109I	RGGNCCY	2 cut(s)	871, 1269
EcoO65I	GGTNACC	1 cut(s)	91
EcoRI	GAATTC	2 cut(s)	19, 158
EcoRII	CCWGG	2 cut(s)	184, 982
EcoT14I	CCWWGG	1 cut(s)	683
ErhI	CCWWGG	1 cut(s)	683
Esp3I	CGTCTC	1 cut(s)	388
FaqI	GGGAC	2 cut(s)	562, 857
FauI	CCCGC	1 cut(s)	1028
FbaI	TGATCA	1 cut(s)	819
FblI	GTMKAC	1 cut(s)	1280
FokI	GGATG	2 cut(s)	794, 1045
FspBI	CTAG	4 cut(s)	98, 170, 302, 1474
HaeIII	GGCC	1 cut(s)	327
HapII	CCGG	6 cut(s)	546, 590, 613, 705, 887, 1112
HgaI	GACGC	1 cut(s)	110
Hin1I	GRCGYC	1 cut(s)	102
HindIII	AAGCTT	1 cut(s)	413
HinfI	GANTC	6 cut(s)	70, 631, 661, 797, 827, 987
HpaII	CCGG	6 cut(s)	546, 590, 613, 705, 887, 1112
HphI	GGTGA	3 cut(s)	370, 704, 829
Hpy166II	GTNNAC	6 cut(s)	369, 635, 754, 1135, 1281, 1457
Hpy188I	TCNGA	3 cut(s)	212, 660, 924
Hpy188III	TCNNGA	5 cut(s)	250, 761, 831, 1087, 1289
Hpy8I	GTNNAC	6 cut(s)	369, 635, 754, 1135, 1281, 1457
Hpy99I	CGWCG	1 cut(s)	140
HpyAV	CCTTC	2 cut(s)	1073, 1099
HpyCH4III	ACNGT	6 cut(s)	49, 203, 382, 790, 846, 1349
HpyCH4IV	ACGT	3 cut(s)	138, 741, 1129
HpyCH4V	TGCA	9 cut(s)	167, 257, 314, 972, 1003, 1191, 1208, 1313, 1424
HpyF10VI	GCNNNNNNNGC	4 cut(s)	311, 538, 892, 1331
HpyF3I	CTNAG	2 cut(s)	125, 1175
HpySE526I	ACGT	3 cut(s)	138, 741, 1129
Hsp92I	GRCGYC	1 cut(s)	102
KflI	GGGWCCC	1 cut(s)	871
Ksp22I	TGATCA	1 cut(s)	819
Kzo9I	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
LweI	GCATC	2 cut(s)	498, 1300
MaeI	CTAG	4 cut(s)	98, 170, 302, 1474
MaeII	ACGT	3 cut(s)	138, 741, 1129
MaeIII	GTNAC	4 cut(s)	91, 203, 742, 935
MalI	GATC	6 cut(s)	318, 544, 689, 821, 891, 1057
MboI	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
MlyI	GAGTC	2 cut(s)	640, 655
MmeI	TCCRAC	2 cut(s)	762, 1074
MroXI	GAANNNNTTC	2 cut(s)	557, 1183
MseI	TTAA	4 cut(s)	180, 450, 720, 810
MspI	CCGG	6 cut(s)	546, 590, 613, 705, 887, 1112
MspR9I	CCNGG	6 cut(s)	186, 547, 590, 591, 706, 984
Mva1269I	GAATGC	1 cut(s)	1175
MvaI	CCWGG	2 cut(s)	186, 984
MwoI	GCNNNNNNNGC	4 cut(s)	311, 538, 892, 1331
NciI	CCSGG	4 cut(s)	547, 590, 591, 706
NdeII	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
NlaIV	GGNNCC	5 cut(s)	560, 711, 872, 873, 1110
NspI	RCATGY	3 cut(s)	1161, 1392, 1428
NspV	TTCGAA	1 cut(s)	990
PctI	GAATGC	1 cut(s)	1175
PdmI	GAANNNNTTC	2 cut(s)	557, 1183
PfeI	GAWTC	4 cut(s)	70, 797, 827, 987
PflMI	CCANNNNNTGG	2 cut(s)	249, 417
PleI	GAGTC	2 cut(s)	639, 655
PmaCI	CACGTG	1 cut(s)	1130
PmlI	CACGTG	1 cut(s)	1130
PpsI	GAGTC	2 cut(s)	639, 655
Ppu21I	YACGTR	1 cut(s)	1130
PpuMI	RGGWCCY	2 cut(s)	871, 1269
PshBI	ATTAAT	1 cut(s)	450
Psp1406I	AACGTT	1 cut(s)	741
Psp5II	RGGWCCY	2 cut(s)	871, 1269
Psp6I	CCWGG	2 cut(s)	184, 982
PspCI	CACGTG	1 cut(s)	1130
PspEI	GGTNACC	1 cut(s)	91
PspGI	CCWGG	2 cut(s)	184, 982
PspN4I	GGNNCC	5 cut(s)	560, 711, 872, 873, 1110
PspPI	GGNCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
PspPPI	RGGWCCY	2 cut(s)	871, 1269
RsaI	GTAC	4 cut(s)	46, 221, 611, 787
RsaNI	GTAC	4 cut(s)	45, 220, 610, 786
Rsr2I	CGGWCCG	1 cut(s)	846
RsrII	CGGWCCG	1 cut(s)	846
SaqAI	TTAA	4 cut(s)	180, 450, 720, 810
Sau3AI	GATC	6 cut(s)	316, 542, 687, 819, 889, 1055
Sau96I	GGNCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
ScaI	AGTACT	1 cut(s)	46
SchI	GAGTC	2 cut(s)	640, 655
ScrFI	CCNGG	6 cut(s)	186, 547, 590, 591, 706, 984
SfaNI	GCATC	2 cut(s)	498, 1300
SfuI	TTCGAA	1 cut(s)	990
SinI	GGWCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
SmaI	CCCGGG	1 cut(s)	591
SmlI	CTYRAG	1 cut(s)	759
SmoI	CTYRAG	1 cut(s)	759
SsiI	CCGC	5 cut(s)	456, 673, 849, 956, 1035
SspMI	CTAG	4 cut(s)	98, 170, 302, 1474
StyD4I	CCNGG	6 cut(s)	184, 545, 588, 589, 704, 982
StyI	CCWWGG	1 cut(s)	683
TaaI	ACNGT	6 cut(s)	49, 203, 382, 790, 846, 1349
TaiI	ACGT	3 cut(s)	141, 744, 1132
TaqI	TCGA	1 cut(s)	990
TatI	WGTACW	1 cut(s)	44
TfiI	GAWTC	4 cut(s)	70, 797, 827, 987
Tru1I	TTAA	4 cut(s)	180, 450, 720, 810
Tru9I	TTAA	4 cut(s)	180, 450, 720, 810
TscAI	CASTG	2 cut(s)	160, 902
TspDTI	ATGAA	6 cut(s)	32, 299, 536, 810, 840, 1227
TspGWI	ACGGA	2 cut(s)	391, 1051
TspMI	CCCGGG	1 cut(s)	589
TspRI	CASTG	2 cut(s)	160, 902
Van91I	CCANNNNNTGG	2 cut(s)	249, 417
VpaK11BI	GGWCC	8 cut(s)	346, 366, 680, 702, 846, 871, 920, 1269
VspI	ATTAAT	1 cut(s)	450
XagI	CCTNNNNNAGG	1 cut(s)	128
XapI	RAATTY	6 cut(s)	19, 29, 158, 228, 962, 1461
XceI	RCATGY	3 cut(s)	1161, 1392, 1428
XmaI	CCCGGG	1 cut(s)	589
XmiI	GTMKAC	1 cut(s)	1280
XmnI	GAANNNNTTC	2 cut(s)	557, 1183
XspI	CTAG	4 cut(s)	98, 170, 302, 1474
ZrmI	AGTACT	1 cut(s)	46

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.