RchiOBHm_Chr2g0120011

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000099
Physical Location & Seq
Forward (+)
32714883 .. 32718252
3370 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49266

Sequence Viewer

Length: 2436 bp
ATGAATTCTATTCAATGGTCTATGATCATTGCATTGCTCATCTTCCTTCTTTCTCTCCCCTCTTGCATTTCCAATGATTCCATTACTCCAAACCAAACCATCAAAGATGGCGACGTTTTAGTCTCAAGTAGGAAACACTTTGCATTAGGGTTCTTCAGCCCTGGAAATTATGGCAAACGTTACGTTGGAGTATGGTATAACAAATTAAGTTCTAGAGCATACCATTGTTTGGATGGAGGCCTTGTCATATATGGAAAGGACAGAAAACTCCCCCTTTGGTCAGCTAATGTTACTCTCTCTTCTTCAAAAAACTCCATGGCCAAGCTGTTGGATACAGGTAACTTCGTTTTGTTTGAGAATGGCCGGAGCCTGTGGGAACAGTTTGATTATCCCTCAAATACAATGCTTCCCTTTATGAAAATTGGGCTGAACCGGCGATCCGGGCTGAACCGGTTCCTAACTTCTTGGAAGTCCCAAGATGATCCGGGAACCGGGAATTTCTCATATAGGATTGATCCGGATGGGTTTCCGCAGCTGTTTTTGTACAGCGGTCAAGTTCCTCGATGGCGGGCCGGATCTTGGATCGGACACAGATGGAGTGGTATCCCCGAAATGACTCGTAATTTCATCTTCAACACTAGTTTTGTGAACAATCAAGATGAGCTATCCATCATGACTGGCATTAAGGACGACTCAATCTTCTCAAGGATGGTGCTTGATGAATCGGGAATCATCGAACGGTCCAAGTGGCAAGATCAGGATCACCAATGGAACAAGTACTGGCATGCCCCGGCAGAACTATGTGACGACTATGGAAAGTGTGGTCCAAATAGCAACTGTGACCCTTACAATGCGGATGAGTTCGAGTGTACATGCCTACCTGGGTTTGAACCTAAGTTGCTCCGTGCATGGTACTTGAGAGATGGCTCGGATGGATGTGTGAGGAAAAAAGGAGCATCTGTGTGTAAAAACGGAGAAGGGTTCGTGAAGGTGTCATCAGTAAAGGCACCAGACTCATCTACTGCGCAGGTAAACTTGACCATGGGTCTGAAAGAATGTGAAAAGGAATGCATAAGGAATTGTTCTTGCATGGCATACTCGAGTGCAGGTGACCCGCAAGAAAGGAATGGATGCGTGACATGGTACGGGGACTTGATGGATACAAGGACATACACGAATGTCAATCAAGATTTATATGTTCGAGTTGATTCAATTGAATTAGCTAAACATGCAAAGAATGGTTCTCTTAGCAAGAAGGGAAAGCTGGCAATTATGATAACATCTATTTTAGTGTTCTTTGTTGTGATCTCTATCGCATATTGGTCCACAAAGATGAAGAGAAAAGGTACGGAAAGACAAAATCTATTTTCATTTGGTGTGATCACACCTTCAGTCTACTTGGAAGATTTTTCTAGTAAAACAGATCCCGATGATAGCAGATTAAAGTCGGATATACCTTTCTTTGATCGAAGCACCATATCTGCAACCACAAACAATTTCTCTTATTCAAACAGGCTTGGAGAAGGCGGATTCGGCCCCGTTTATAAGGGAGTGCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTAAGCAAAAATTCTGGCCAGGGAAATGAAGAGTTTAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTGAGAATCTTAGGCTATTGCATTCAAGATAAAGAGAAAATGCTAATCTATGAATACATGCCTAACAAAAGCCTGGACTCATTCATTTTCAATGAAATGAAAAGAACATCTCTGGATTGGACAAGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGGATCTTGTATCTTCATCAAGATTCGAGATTAAGAATCATCCACAGAGACCTAAAGGCCAGCAATATTCTACTGGATTATGATATGAACCCCAAAATTGCAGATTTTGGTGTGGCTAGAATATTCGAAGCAAACCAAATTGAAGCAAATACAAATCGCGTGGTTGGAACATATGGTTACATGTCACCAGAGTATGCAATGAAAGGACTATTTTCAGTGAAGTCGGATGTATACAGTTTCGGTGTTTTGCTGCTAGAAATCGTTATTGGTAGAAAGAACACTGATTACTACTGTGATAATCCTGACTCAAATTTGGTTGGACATGTTTGGGAATTGTGGAATGAAGGCCGAGCCTTGGAAATCATCGATACATCTTTGGGTGAATCCTACCCCATAAGTGAAGTTCAACGGTGCATTCAAATCGCGCTCCTGTGCGTGCAAGAGTACGCAAATGACCGGCCAACCATGTCAAAAAATTTGTCTGTGTTAGGCAACGATTCAGCTCTTCCTTCACCAAGAATACCTGGATTTCTGTTGAATAGAAGTAACTATACCAGTGGAGACCCCAGTGCTAGTACTGGAGATGCTAATTCCATAAATTATGTAACATGA

Protein Analysis

811

Amino Acids

91.55

Weight (kDa)

7.95

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 157 3.4e-19 D-mannose binding lectin
S_locus_glycop PF00954 189 - 299 7.2e-29 S-locus glycoprotein domain
PAN_2 PF08276 322 - 388 3e-18 PAN-like domain
PK_Tyr_Ser-Thr PF07714 502 - 766 5.6e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 502 - 765 2.4e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1545
AarI CACCTGC 1 cut(s) 1097
AasI GACNNNNNNGTC 1 cut(s) 119
Acc16I TGCGCA 1 cut(s) 1026
Acc36I ACCTGC 2 cut(s) 1018, 1097
AccB1I GGYRCC 1 cut(s) 1006
AccB7I CCANNNNNTGG 1 cut(s) 229
AccI GTMKAC 2 cut(s) 1395, 2055
AccII CGCG 2 cut(s) 1983, 2249
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 6 cut(s) 530, 549, 568, 854, 1115, 1527
AclI AACGTT 1 cut(s) 178
AclWI GGATC 8 cut(s) 432, 476, 509, 583, 590, 768, 1418, 1835
AcoI YGGCCR 4 cut(s) 318, 361, 1603, 2282
AcsI RAATTY 5 cut(s) 4, 496, 1597, 2134, 2299
AcuI CTGAAG 2 cut(s) 139, 1374
AfaI GTAC 8 cut(s) 545, 779, 871, 914, 1145, 1348, 2270, 2401
AfiI CCNNNNNNNGG 4 cut(s) 229, 491, 579, 2179
AflIII ACRYGT 2 cut(s) 2004, 2146
AgeI ACCGGT 1 cut(s) 450
AhdI GACNNNNNGTC 1 cut(s) 1044
AhlI ACTAGT 1 cut(s) 638
AjnI CCWGG 5 cut(s) 160, 880, 1605, 1740, 2347
AjuI GAANNNNNNNTTGG 2 cut(s) 2073, 2105
AloI GAACNNNNNNTCC 2 cut(s) 422, 454
AluBI AGCT 7 cut(s) 284, 325, 535, 664, 1223, 1264, 2327
AluI AGCT 7 cut(s) 284, 325, 535, 664, 1223, 1264, 2327
Alw26I GTCTC 3 cut(s) 127, 1866, 2379
AlwI GGATC 8 cut(s) 432, 476, 509, 583, 590, 768, 1418, 1835
Ama87I CYCGRG 1 cut(s) 1099
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 9 cut(s) 238, 318, 361, 570, 1534, 1603, 1881, 2170, 2282
ApeKI GCWGC 2 cut(s) 532, 2074
ApoI RAATTY 5 cut(s) 4, 496, 1597, 2134, 2299
ArsI GACNNNNNNTTYG 2 cut(s) 2288, 2320
AsiGI ACCGGT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 1027, 2251
AspS9I GGNCC 5 cut(s) 570, 741, 824, 1323, 1535
AsuC2I CCSGG 4 cut(s) 442, 486, 493, 791
AsuHPI GGTGA 5 cut(s) 755, 1121, 2001, 2216, 2328
AsuII TTCGAA 1 cut(s) 1950
AvaI CYCGRG 1 cut(s) 1099
AvaII GGWCC 3 cut(s) 741, 824, 1323
BalI TGGCCA 2 cut(s) 320, 1605
BanI GGYRCC 1 cut(s) 1006
BarI GAAGNNNNNNTAC 2 cut(s) 2359, 2391
BbvI GCAGC 2 cut(s) 544, 2061
BceAI ACGGC 1 cut(s) 1562
BcgI CGANNNNNNTGC 2 cut(s) 2227, 2261
BciT130I CCWGG 5 cut(s) 162, 882, 1607, 1742, 2349
BciVI GTATCC 3 cut(s) 325, 614, 1153
BclI TGATCA 2 cut(s) 24, 1380
BcnI CCSGG 4 cut(s) 442, 486, 493, 791
BcoDI GTCTC 3 cut(s) 127, 1866, 2379
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 8 cut(s) 213, 639, 1413, 1560, 1821, 1941, 2078, 2397
BfuAI ACCTGC 2 cut(s) 1018, 1097
BfuI GTATCC 3 cut(s) 325, 614, 1153
BisI GCNGC 2 cut(s) 533, 2075
BlsI GCNGC 2 cut(s) 534, 2076
BmcAI AGTACT 2 cut(s) 779, 2401
Bme1390I CCNGG 9 cut(s) 162, 442, 486, 493, 791, 882, 1607, 1742, 2349
Bme18I GGWCC 3 cut(s) 741, 824, 1323
BmeRI GACNNNNNGTC 1 cut(s) 1044
BmeT110I CYCGRG 1 cut(s) 1099
BmgT120I GGNCC 5 cut(s) 570, 741, 824, 1323, 1535
BmiI GGNNCC 6 cut(s) 368, 455, 490, 1008, 1537, 1664
BmrFI CCNGG 9 cut(s) 162, 442, 486, 493, 791, 882, 1607, 1742, 2349
BmrI ACTGGG 1 cut(s) 2385
BmsI GCATC 3 cut(s) 965, 1121, 2398
BmuI ACTGGG 1 cut(s) 2385
BpmI CTGGAG 1 cut(s) 2424
Bpu14I TTCGAA 1 cut(s) 1950
BpuEI CTTGAG 3 cut(s) 109, 688, 937
BpuMI CCSGG 4 cut(s) 442, 486, 493, 791
Bsa29I ATCGAT 1 cut(s) 2190
BsaBI GATNNNNATC 2 cut(s) 759, 1310
BsaI GGTCTC 2 cut(s) 1866, 2379
BsaJI CCNNGG 7 cut(s) 160, 315, 789, 881, 1041, 1606, 2178
BsaWI WCCGGW 2 cut(s) 450, 517
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 945, 975
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 491, 579, 2179
Bse118I RCCGGY 3 cut(s) 432, 450, 2280
Bse1I ACTGG 6 cut(s) 682, 785, 1902, 2379, 2391, 2407
Bse3DI GCAATG 4 cut(s) 27, 32, 1815, 2028
Bse8I GATNNNNATC 2 cut(s) 759, 1310
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 5 cut(s) 162, 882, 1607, 1742, 2349
BseCI ATCGAT 1 cut(s) 2190
BseDI CCNNGG 7 cut(s) 160, 315, 789, 881, 1041, 1606, 2178
BseGI GGATG 9 cut(s) 238, 526, 714, 862, 937, 941, 1136, 1863, 2056
BseJI GATNNNNATC 2 cut(s) 759, 1310
BseLI CCNNNNNNNGG 4 cut(s) 229, 491, 579, 2179
BseMI GCAATG 4 cut(s) 27, 32, 1815, 2028
BseNI ACTGG 6 cut(s) 682, 785, 1902, 2379, 2391, 2407
BseXI GCAGC 2 cut(s) 544, 2061
BsgI GTGCAG 1 cut(s) 1125
Bsh1236I CGCG 2 cut(s) 1983, 2249
BshFI GGCC 9 cut(s) 240, 320, 363, 572, 1536, 1605, 1883, 2172, 2284
BshNI GGYRCC 1 cut(s) 1006
BshTI ACCGGT 1 cut(s) 450
BshVI ATCGAT 1 cut(s) 2190
BsiHKCI CYCGRG 1 cut(s) 1099
BslFI GGGAC 2 cut(s) 457, 1163
BslI CCNNNNNNNGG 4 cut(s) 229, 491, 579, 2179
BsmAI GTCTC 3 cut(s) 127, 1866, 2379
BsmFI GGGAC 2 cut(s) 457, 1163
BsmI GAATGC 3 cut(s) 1073, 1689, 2238
BsnI GGCC 9 cut(s) 240, 320, 363, 572, 1536, 1605, 1883, 2172, 2284
Bso31I GGTCTC 2 cut(s) 1866, 2379
BsoBI CYCGRG 1 cut(s) 1099
Bsp119I TTCGAA 1 cut(s) 1950
Bsp13I TCCGGA 1 cut(s) 517
Bsp1407I TGTACA 2 cut(s) 543, 869
Bsp19I CCATGG 2 cut(s) 315, 1041
BspACI CCGC 6 cut(s) 530, 549, 568, 854, 1115, 1527
BspANI GGCC 9 cut(s) 240, 320, 363, 572, 1536, 1605, 1883, 2172, 2284
BspDI ATCGAT 1 cut(s) 2190
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 2 cut(s) 1983, 2249
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 6 cut(s) 368, 455, 490, 1008, 1537, 1664
BspMI ACCTGC 2 cut(s) 1018, 1097
BspPI GGATC 8 cut(s) 432, 476, 509, 583, 590, 768, 1418, 1835
BspQI GCTCTTC 1 cut(s) 2334
BspT104I TTCGAA 1 cut(s) 1950
BspT107I GGYRCC 1 cut(s) 1006
BspTNI GGTCTC 2 cut(s) 1866, 2379
BsrDI GCAATG 4 cut(s) 27, 32, 1815, 2028
BsrFI RCCGGY 3 cut(s) 432, 450, 2280
BsrGI TGTACA 2 cut(s) 543, 869
BsrI ACTGG 6 cut(s) 682, 785, 1902, 2379, 2391, 2407
BssAI RCCGGY 3 cut(s) 432, 450, 2280
BssECI CCNNGG 7 cut(s) 160, 315, 789, 881, 1041, 1606, 2178
BssNAI GTATAC 1 cut(s) 2056
BssT1I CCWWGG 3 cut(s) 315, 1041, 2178
Bst1107I GTATAC 1 cut(s) 2056
Bst2UI CCWGG 5 cut(s) 162, 882, 1607, 1742, 2349
Bst4CI ACNGT 6 cut(s) 381, 741, 839, 2060, 2117, 2235
Bst6I CTCTTC 4 cut(s) 304, 1331, 1611, 2334
BstAUI TGTACA 2 cut(s) 543, 869
BstBI TTCGAA 1 cut(s) 1950
BstC8I GCNNGC 5 cut(s) 570, 786, 1266, 1885, 2261
BstDEI CTNAG 4 cut(s) 894, 1247, 1589, 1678
BstDSI CCRYGG 2 cut(s) 315, 1041
BstEII GGTNACC 1 cut(s) 1109
BstF5I GGATG 9 cut(s) 238, 526, 714, 862, 937, 941, 1136, 1863, 2056
BstFNI CGCG 2 cut(s) 1983, 2249
BstHHI GCGC 2 cut(s) 1027, 2251
BstMAI GTCTC 3 cut(s) 127, 1866, 2379
BstMWI GCNNNNNNNGC 4 cut(s) 433, 442, 1229, 1533
BstNI CCWGG 5 cut(s) 162, 882, 1607, 1742, 2349
BstNSI RCATGY 6 cut(s) 788, 876, 1232, 1729, 2008, 2150
BstPI GGTNACC 1 cut(s) 1109
BstSCI CCNGG 9 cut(s) 160, 440, 484, 491, 789, 880, 1605, 1740, 2347
BstUI CGCG 2 cut(s) 1983, 2249
BstV1I GCAGC 2 cut(s) 544, 2061
BstX2I RGATCY 3 cut(s) 575, 1423, 1827
BstXI CCANNNNNNTGG 1 cut(s) 328
BstYI RGATCY 3 cut(s) 575, 1423, 1827
BstZ17I GTATAC 1 cut(s) 2056
Bsu15I ATCGAT 1 cut(s) 2190
BsuI GTATCC 3 cut(s) 325, 614, 1153
BsuRI GGCC 9 cut(s) 240, 320, 363, 572, 1536, 1605, 1883, 2172, 2284
BsuTUI ATCGAT 1 cut(s) 2190
BtgI CCRYGG 2 cut(s) 315, 1041
BtsCI GGATG 9 cut(s) 238, 526, 714, 862, 937, 941, 1136, 1863, 2056
BtsIMutI CAGTG 4 cut(s) 2046, 2103, 2386, 2398
BveI ACCTGC 2 cut(s) 1018, 1097
Cac8I GCNNGC 5 cut(s) 570, 786, 1266, 1885, 2261
CciI TCATGA 1 cut(s) 672
CfoI GCGC 2 cut(s) 1027, 2251
Cfr10I RCCGGY 3 cut(s) 432, 450, 2280
Cfr13I GGNCC 5 cut(s) 570, 741, 824, 1323, 1535
ClaI ATCGAT 1 cut(s) 2190
CseI GACGC 1 cut(s) 1803
Csp6I GTAC 8 cut(s) 544, 778, 870, 913, 1144, 1347, 2269, 2400
CspAI ACCGGT 1 cut(s) 450
CspCI CAANNNNNGTGG 2 cut(s) 1965, 2000
CviQI GTAC 8 cut(s) 544, 778, 870, 913, 1144, 1347, 2269, 2400
DdeI CTNAG 4 cut(s) 894, 1247, 1589, 1678
DrdI GACNNNNNNGTC 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 1044
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 4 cut(s) 318, 361, 1603, 2282
Eam1104I CTCTTC 4 cut(s) 304, 1331, 1611, 2334
Eam1105I GACNNNNNGTC 1 cut(s) 1044
EarI CTCTTC 4 cut(s) 304, 1331, 1611, 2334
EciI GGCGGA 1 cut(s) 1542
Eco130I CCWWGG 3 cut(s) 315, 1041, 2178
Eco147I AGGCCT 1 cut(s) 240
Eco31I GGTCTC 2 cut(s) 1866, 2379
Eco47I GGWCC 3 cut(s) 741, 824, 1323
Eco57I CTGAAG 2 cut(s) 139, 1374
Eco88I CYCGRG 1 cut(s) 1099
Eco91I GGTNACC 1 cut(s) 1109
EcoO65I GGTNACC 1 cut(s) 1109
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 5 cut(s) 160, 880, 1605, 1740, 2347
EcoT14I CCWWGG 3 cut(s) 315, 1041, 2178
EcoT22I ATGCAT 1 cut(s) 1073
ErhI CCWWGG 3 cut(s) 315, 1041, 2178
FaqI GGGAC 2 cut(s) 457, 1163
FauI CCCGC 2 cut(s) 561, 1122
FauNDI CATATG 1 cut(s) 1996
FbaI TGATCA 2 cut(s) 24, 1380
FblI GTMKAC 2 cut(s) 1395, 2055
Fnu4HI GCNGC 2 cut(s) 533, 2075
FokI GGATG 9 cut(s) 245, 533, 721, 869, 944, 948, 1143, 1850, 2063
Fsp4HI GCNGC 2 cut(s) 533, 2075
FspBI CTAG 8 cut(s) 213, 639, 1413, 1560, 1821, 1941, 2078, 2397
FspI TGCGCA 1 cut(s) 1026
GlaI GCGC 2 cut(s) 1026, 2250
GluI GCNGC 2 cut(s) 533, 2075
GsuI CTGGAG 1 cut(s) 2424
HaeIII GGCC 9 cut(s) 240, 320, 363, 572, 1536, 1605, 1883, 2172, 2284
HgaI GACGC 1 cut(s) 1803
HhaI GCGC 2 cut(s) 1027, 2251
Hin6I GCGC 2 cut(s) 1025, 2249
HinP1I GCGC 2 cut(s) 1025, 2249
HphI GGTGA 5 cut(s) 755, 1121, 2001, 2216, 2328
Hpy166II GTNNAC 6 cut(s) 649, 870, 1033, 1326, 1396, 2056
Hpy188I TCNGA 5 cut(s) 587, 931, 1050, 1450, 2050
Hpy8I GTNNAC 6 cut(s) 649, 870, 1033, 1326, 1396, 2056
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 8 cut(s) 56, 971, 982, 1249, 1398, 1517, 2162, 2343
HpyCH4III ACNGT 6 cut(s) 381, 741, 839, 2060, 2117, 2235
HpyCH4IV ACGT 3 cut(s) 114, 178, 183
HpyF10VI GCNNNNNNNGC 4 cut(s) 433, 442, 1229, 1533
HpyF3I CTNAG 4 cut(s) 894, 1247, 1589, 1678
HpySE526I ACGT 3 cut(s) 114, 178, 183
HspAI GCGC 2 cut(s) 1025, 2249
Kpn2I TCCGGA 1 cut(s) 517
Ksp22I TGATCA 2 cut(s) 24, 1380
LguI GCTCTTC 1 cut(s) 2334
LmnI GCTCC 4 cut(s) 366, 906, 953, 2256
Lsp1109I GCAGC 2 cut(s) 544, 2061
LweI GCATC 3 cut(s) 965, 1121, 2398
MaeI CTAG 8 cut(s) 213, 639, 1413, 1560, 1821, 1941, 2078, 2397
MaeII ACGT 3 cut(s) 114, 178, 183
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 3 cut(s) 575, 1423, 1827
MlsI TGGCCA 2 cut(s) 320, 1605
MluNI TGGCCA 2 cut(s) 320, 1605
MlyI GAGTC 5 cut(s) 610, 686, 1007, 1739, 2123
MmeI TCCRAC 6 cut(s) 166, 309, 1428, 1969, 2028, 2122
MnlI CCTC 6 cut(s) 70, 230, 403, 570, 936, 1817
Mox20I TGGCCA 2 cut(s) 320, 1605
Mph1103I ATGCAT 1 cut(s) 1073
MroI TCCGGA 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 2 cut(s) 320, 1605
MseI TTAA 5 cut(s) 206, 684, 1442, 1623, 1856
MslI CAYNNNNRTG 2 cut(s) 961, 1331
Msp20I TGGCCA 2 cut(s) 320, 1605
MspA1I CMGCKG 2 cut(s) 535, 549
MspR9I CCNGG 9 cut(s) 162, 442, 486, 493, 791, 882, 1607, 1742, 2349
MunI CAATTG 1 cut(s) 1212
Mva1269I GAATGC 3 cut(s) 1073, 1689, 2238
MvaI CCWGG 5 cut(s) 162, 882, 1607, 1742, 2349
MvnI CGCG 2 cut(s) 1983, 2249
MwoI GCNNNNNNNGC 4 cut(s) 433, 442, 1229, 1533
NciI CCSGG 4 cut(s) 442, 486, 493, 791
NcoI CCATGG 2 cut(s) 315, 1041
NdeI CATATG 1 cut(s) 1996
NlaIV GGNNCC 6 cut(s) 368, 455, 490, 1008, 1537, 1664
NmeAIII GCCGAG 1 cut(s) 2198
NmuCI GTSAC 5 cut(s) 803, 839, 1109, 1135, 2007
NsbI TGCGCA 1 cut(s) 1026
NsiI ATGCAT 1 cut(s) 1073
NspI RCATGY 6 cut(s) 788, 876, 1232, 1729, 2008, 2150
NspV TTCGAA 1 cut(s) 1950
PaeI GCATGC 1 cut(s) 788
PaeR7I CTCGAG 1 cut(s) 1099
PagI TCATGA 1 cut(s) 672
PaqCI CACCTGC 1 cut(s) 1097
PceI AGGCCT 1 cut(s) 240
PciI ACATGT 2 cut(s) 2004, 2146
PciSI GCTCTTC 1 cut(s) 2334
PctI GAATGC 3 cut(s) 1073, 1689, 2238
PdmI GAANNNNTTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 229
PfoI TCCNGGA 1 cut(s) 484
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 2 cut(s) 534, 2076
PleI GAGTC 5 cut(s) 610, 686, 1007, 1739, 2123
PpsI GAGTC 5 cut(s) 610, 686, 1007, 1739, 2123
PscI ACATGT 2 cut(s) 2004, 2146
PsiI TTATAA 1 cut(s) 1545
Psp1406I AACGTT 1 cut(s) 178
Psp6I CCWGG 5 cut(s) 160, 880, 1605, 1740, 2347
PspEI GGTNACC 1 cut(s) 1109
PspGI CCWGG 5 cut(s) 160, 880, 1605, 1740, 2347
PspN4I GGNNCC 6 cut(s) 368, 455, 490, 1008, 1537, 1664
PspPI GGNCC 5 cut(s) 570, 741, 824, 1323, 1535
PspXI VCTCGAGB 1 cut(s) 1099
PsrI GAACNNNNNNTAC 2 cut(s) 2093, 2125
PsuI RGATCY 3 cut(s) 575, 1423, 1827
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 8 cut(s) 545, 779, 871, 914, 1145, 1348, 2270, 2401
RsaNI GTAC 8 cut(s) 544, 778, 870, 913, 1144, 1347, 2269, 2400
RseI CAYNNNNRTG 2 cut(s) 961, 1331
SapI GCTCTTC 1 cut(s) 2334
SaqAI TTAA 5 cut(s) 206, 684, 1442, 1623, 1856
SatI GCNGC 2 cut(s) 533, 2075
Sau96I GGNCC 5 cut(s) 570, 741, 824, 1323, 1535
ScaI AGTACT 2 cut(s) 779, 2401
SchI GAGTC 5 cut(s) 610, 686, 1007, 1739, 2123
ScrFI CCNGG 9 cut(s) 162, 442, 486, 493, 791, 882, 1607, 1742, 2349
SfaNI GCATC 3 cut(s) 965, 1121, 2398
Sfr274I CTCGAG 1 cut(s) 1099
SfuI TTCGAA 1 cut(s) 1950
SinI GGWCC 3 cut(s) 741, 824, 1323
SlaI CTCGAG 1 cut(s) 1099
SmiMI CAYNNNNRTG 2 cut(s) 961, 1331
SmlI CTYRAG 4 cut(s) 124, 703, 916, 1099
SmoI CTYRAG 4 cut(s) 124, 703, 916, 1099
SpeI ACTAGT 1 cut(s) 638
SphI GCATGC 1 cut(s) 788
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 6 cut(s) 530, 549, 568, 854, 1115, 1527
SspI AATATT 2 cut(s) 1891, 1947
SspMI CTAG 8 cut(s) 213, 639, 1413, 1560, 1821, 1941, 2078, 2397
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 9 cut(s) 160, 440, 484, 491, 789, 880, 1605, 1740, 2347
StyI CCWWGG 3 cut(s) 315, 1041, 2178
TaaI ACNGT 6 cut(s) 381, 741, 839, 2060, 2117, 2235
TaiI ACGT 3 cut(s) 117, 181, 186
TaqI TCGA 9 cut(s) 562, 735, 864, 1100, 1201, 1468, 1850, 1950, 2190
TatI WGTACW 4 cut(s) 543, 777, 869, 2399
Tru1I TTAA 5 cut(s) 206, 684, 1442, 1623, 1856
Tru9I TTAA 5 cut(s) 206, 684, 1442, 1623, 1856
TscAI CASTG 4 cut(s) 2046, 2110, 2386, 2398
TseFI GTSAC 5 cut(s) 803, 839, 1109, 1135, 2007
TseI GCWGC 2 cut(s) 532, 2074
Tsp45I GTSAC 5 cut(s) 803, 839, 1109, 1135, 2007
TspGWI ACGGA 3 cut(s) 893, 987, 1364
TspRI CASTG 4 cut(s) 2046, 2110, 2386, 2398
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 3 cut(s) 741, 824, 1323
XapI RAATTY 5 cut(s) 4, 496, 1597, 2134, 2299
XbaI TCTAGA 1 cut(s) 212
XceI RCATGY 6 cut(s) 788, 876, 1232, 1729, 2008, 2150
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XhoI CTCGAG 1 cut(s) 1099
XmiI GTMKAC 2 cut(s) 1395, 2055
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 8 cut(s) 213, 639, 1413, 1560, 1821, 1941, 2078, 2397
ZrmI AGTACT 2 cut(s) 779, 2401
Zsp2I ATGCAT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.