RchiOBHm_Chr2g0120061

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000099
Physical Location & Seq
Forward (+)
32742113 .. 32745489
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49271

Sequence Viewer

Length: 2511 bp
ATGAATGCTATTCAATGGTCTATGATCACTGCATTGCTCATCTTCCTGCTTTATCTCCCCTCTTGCATTTCCATTGAATCCATTACTCCAAACCAAACCATCAAAGACGGCGAGGTTTTAGTCTCGAGCAGGAAGCTCTTTGCGCTAGGGTTCTTCAGCCCTGGAAATTCTGGCAGACGTTACGTTGGAGTTTGGTATAACAAAGTTCCAGAGCAGACCATTGTTTGGGTTGCAAATAGAGACAACCCTGTCAATGATTCCTCTGGACTTCTAGTGATCGAGGGAGATGGAGGCCTTGTCATATATGGAAAGGACGGAAAACTCCCTCTTTGGTCAGCTAATGTTACTCTCTCTTCTCCAAAAAACTCCATGGCCAAGCTGTTGGATACAGGTAACTTTGTTTTGTTTGAGAATGGGCGGAGCCTGTGGGAAGGGTTTGATTATCCCTCAAACACAATGCTTCCCTTTATGAAAATTGGGCTGAACCGGCGATCCGGGCTGAACCGGTTCCTAACTTCTTGGAAGTCCCAAGATGATCCGGGAACCGGGAATTTCTCATTTAGGATTGATCCGGATGGGTTTCCGCAGCTGGTTTTGTACAGCGGTCAAGCTCCCCGATGGCGGGCCGGATCTTGGATCGGACACAGATGGAGTGGTATCCCCGAAATGACTCGTAATTTCATCTTCAACACTAGTTTTGTGAACAATCAAGATGAGCTATCCGTCATGACTGGCATTAAGGACGACTCAATCTTCTCAAGGATGGTGCTTGATGAATCGGGAATCATCGAACGGTCCATGTGGCAAGATCAGGATCACCGATGGAACAAGTACTGGCATGCCCCGGCAGAACTATGTGACGACTACGGAAAGTGTGGTCCAAATAGCAACTGTGACCCTTACAATGCGGATGAGTTCGAGTGTACATGCCTACCTGGGTTTGAACCTAAGTTGCTCCGTGCATGGTACTTGAGAGATGGCTCGGATGGATGTGTGAGGAAAAAAGGAGCATCCGTGTGTAAAAACGGAGAAGGGTTCGTGAAGGTGTCACCACTAAAGGCACCAGACTCATCTACTGCGCATGTAAACTTGACCATGGGTCCCAAAGAATGTGAAGAGGAATGCATAAGGAATTGTTCTTGCATGGCATACTCGAGTGCAGGTGACCCGCAAGAAAGGAATGGATGCGTGACATGGTACGGGGACTTGATGGATACAAGGACTTACACGAATGTCAATCAAGATTTATATGTTCGAGTTGATTCAATTGAATTAGCTAAACATGCAAAGAATGGTTCTCTTAGCAAGAAGGGAAAGCTGGCAATTACGATAACATCTATTTTAGTGTTCTTTCTTGTGATCTCTATCGCATATTGGTCCATAAAGATGAAGAGAAAAGGTAAGGAAAGACAAAATCCATTTCCATTTGGTGTCATCACAACTTCAATCTACTTGGAAGATTCTCCTAGTAGAACAGATCCTGACGGTAGCAGATTCAAGTCGGATATACCTTTCTTTGATCTAAGCACCATATCTACAGCCACAAAAAATTTCTCAGATTCAAACAAGCTTGGAGAAGGCGGATTTGGCCCCGTTTATAAGGGAGTGCTTTCTAGTGGAACAGAAATAGCCGTGAAAAGACTGAGCAAAAATTCTGGCCAGGGAAATGAAGAGTTCAAGAATGAAGTTGTGCTGATTGCAAAACTTCAACACAGGAACCTTGTCAGAATCTTAGGCTATTGCGTTCAAGATGAAGAGAAGATGCTAATCTATGAATACCTACCTAACAAAAGCCTGGACTCTTTCATTTTCAATGAACAGAAAAGGACATTTCTGGATTGGACAAGACGCTTGGAGATTATCTTTGGCATTGCTAGAGGGATCTTGTATCTTCATCAAGATTCGAGATTAAGAATCATCCATAGAGACCTAAAGGCCAGCAATGTTCTACTGGATTATGATATGAACCCCAAACTTGCAGATTTTGGTATGGCTAGAATATTCGGAGCAAACCAAATTGAAGCAAATACAAATCGCGTGGTTGGAACATATGGTTACATGTCACCAGAGTATGCAATGGAAGGACTATTTTCAGTGAAGTCTGATGTCTACAGTTTCGGTGTTTTGCTGCTAGAAATTGTTACTGGCAGAAAGAACACCGGTTACTACTCTGATAATCCTGACTCAAATTTGGTTGGACATGTTTGGGACTTGTGGAAAGAAGGCCGAGCCTTGGAAATCGTTGACCCATCTTTGGGAGAATCCTATCCCATATGTGAAGTTCTACGGTGCATTCAAATCGCACTCTTGTGTGTGGAAGAGTACTCAAATGACCGGCCAACCATGTCAAAAGTTGTGTCTCTGTTAGGCAACGATGCAGCTCTTCCTTCACCAAGAAAACCTGGATTTCTGTTGAAGAGAAGTAACTATACCAATGGAGACCCCAGTACTAGTACTAGAGATGCTAATTCCATAAATTATGTTACATGCAGTATAGTTGAAGCCCGCTAA

Protein Analysis

836

Amino Acids

93.89

Weight (kDa)

6.56

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 175 5.1e-31 D-mannose binding lectin
S_locus_glycop PF00954 207 - 317 1.6e-28 S-locus glycoprotein domain
PAN_2 PF08276 340 - 406 2e-17 PAN-like domain
Pkinase PF00069 519 - 721 1.4e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 521 - 788 9.6e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1599
AarI CACCTGC 1 cut(s) 1151
AasI GACNNNNNNGTC 1 cut(s) 248
Acc16I TGCGCA 1 cut(s) 1080
Acc36I ACCTGC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 1060
AccB7I CCANNNNNTGG 1 cut(s) 225
AccI GTMKAC 1 cut(s) 2109
AccII CGCG 1 cut(s) 2037
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 8 cut(s) 418, 584, 603, 622, 908, 1169, 1581, 2506
AclWI GGATC 8 cut(s) 486, 530, 563, 637, 644, 822, 1472, 1889
AcoI YGGCCR 3 cut(s) 372, 1657, 2336
AcsI RAATTY 5 cut(s) 166, 550, 1549, 1651, 2188
AcuI CTGAAG 1 cut(s) 139
AfaI GTAC 8 cut(s) 599, 833, 925, 968, 1199, 2324, 2449, 2455
AfiI CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2233, 2254, 2255
AflIII ACRYGT 2 cut(s) 2058, 2200
AgeI ACCGGT 2 cut(s) 504, 2159
AhdI GACNNNNNGTC 1 cut(s) 1098
AhlI ACTAGT 2 cut(s) 692, 2450
AjnI CCWGG 5 cut(s) 160, 934, 1659, 1794, 2401
AjuI GAANNNNNNNTTGG 2 cut(s) 1569, 1601
AleI CACNNNNGTG 1 cut(s) 2308
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
Alw26I GTCTC 5 cut(s) 127, 234, 1920, 2364, 2433
AlwI GGATC 8 cut(s) 486, 530, 563, 637, 644, 822, 1472, 1889
AlwNI CAGNNNCTG 1 cut(s) 1481
Ama87I CYCGRG 2 cut(s) 124, 1153
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 8 cut(s) 292, 372, 624, 1588, 1657, 1935, 2224, 2336
ApeKI GCWGC 3 cut(s) 586, 2128, 2378
ApoI RAATTY 5 cut(s) 166, 550, 1549, 1651, 2188
ArsI GACNNNNNNTTYG 2 cut(s) 2342, 2374
AsiGI ACCGGT 2 cut(s) 504, 2159
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 2 cut(s) 145, 1081
AspS9I GGNCC 6 cut(s) 624, 795, 878, 1100, 1377, 1589
AsuC2I CCSGG 4 cut(s) 496, 540, 547, 845
AsuHPI GGTGA 5 cut(s) 809, 1041, 1175, 2055, 2382
AvaI CYCGRG 2 cut(s) 124, 1153
AvaII GGWCC 4 cut(s) 795, 878, 1100, 1377
BalI TGGCCA 2 cut(s) 374, 1659
BanI GGYRCC 1 cut(s) 1060
BarI GAAGNNNNNNTAC 2 cut(s) 2413, 2445
BbvI GCAGC 3 cut(s) 598, 2115, 2390
BceAI ACGGC 2 cut(s) 124, 1616
BcgI CGANNNNNNTGC 2 cut(s) 2281, 2315
BciT130I CCWGG 5 cut(s) 162, 936, 1661, 1796, 2403
BciVI GTATCC 3 cut(s) 379, 668, 1207
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 4 cut(s) 496, 540, 547, 845
BcoDI GTCTC 5 cut(s) 127, 234, 1920, 2364, 2433
BcuI ACTAGT 2 cut(s) 692, 2450
BfmI CTRYAG 2 cut(s) 1536, 2110
BfuAI ACCTGC 1 cut(s) 1151
BfuI GTATCC 3 cut(s) 379, 668, 1207
BisI GCNGC 3 cut(s) 587, 2129, 2379
BlsI GCNGC 3 cut(s) 588, 2130, 2380
BmcAI AGTACT 4 cut(s) 833, 2324, 2449, 2455
Bme1390I CCNGG 9 cut(s) 162, 496, 540, 547, 845, 936, 1661, 1796, 2403
Bme18I GGWCC 4 cut(s) 795, 878, 1100, 1377
BmeRI GACNNNNNGTC 1 cut(s) 1098
BmeT110I CYCGRG 2 cut(s) 124, 1153
BmgT120I GGNCC 6 cut(s) 624, 795, 878, 1100, 1377, 1589
BmiI GGNNCC 8 cut(s) 422, 509, 544, 1062, 1101, 1102, 1591, 1718
BmrFI CCNGG 9 cut(s) 162, 496, 540, 547, 845, 936, 1661, 1796, 2403
BmrI ACTGGG 1 cut(s) 2439
BmsI GCATC 5 cut(s) 1019, 1175, 1752, 2365, 2452
BmuI ACTGGG 1 cut(s) 2439
BpuEI CTTGAG 2 cut(s) 742, 991
BpuMI CCSGG 4 cut(s) 496, 540, 547, 845
BsaBI GATNNNNATC 3 cut(s) 813, 1364, 1766
BsaI GGTCTC 2 cut(s) 1920, 2433
BsaJI CCNNGG 7 cut(s) 160, 369, 843, 935, 1095, 1660, 2232
BsaWI WCCGGW 3 cut(s) 504, 571, 2159
BsaXI ACNNNNNCTCC 4 cut(s) 282, 312, 999, 1029
Bsc4I CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2233, 2254, 2255
Bse118I RCCGGY 4 cut(s) 486, 504, 2159, 2334
Bse1I ACTGG 5 cut(s) 736, 839, 1956, 2149, 2445
Bse3DI GCAATG 4 cut(s) 32, 1869, 1948, 2082
Bse8I GATNNNNATC 3 cut(s) 813, 1364, 1766
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 5 cut(s) 162, 936, 1661, 1796, 2403
BseDI CCNNGG 7 cut(s) 160, 369, 843, 935, 1095, 1660, 2232
BseGI GGATG 8 cut(s) 580, 768, 916, 991, 995, 1010, 1190, 1917
BseJI GATNNNNATC 3 cut(s) 813, 1364, 1766
BseLI CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2233, 2254, 2255
BseMI GCAATG 4 cut(s) 32, 1869, 1948, 2082
BseMII CTCAG 2 cut(s) 1569, 1634
BseNI ACTGG 5 cut(s) 736, 839, 1956, 2149, 2445
BseXI GCAGC 3 cut(s) 598, 2115, 2390
BsgI GTGCAG 1 cut(s) 1179
Bsh1236I CGCG 1 cut(s) 2037
BshFI GGCC 8 cut(s) 294, 374, 626, 1590, 1659, 1937, 2226, 2338
BshNI GGYRCC 1 cut(s) 1060
BshTI ACCGGT 2 cut(s) 504, 2159
BsiHKCI CYCGRG 2 cut(s) 124, 1153
BslFI GGGAC 4 cut(s) 511, 1086, 1217, 2222
BslI CCNNNNNNNGG 8 cut(s) 225, 545, 621, 622, 633, 2233, 2254, 2255
BsmAI GTCTC 5 cut(s) 127, 234, 1920, 2364, 2433
BsmFI GGGAC 4 cut(s) 511, 1086, 1217, 2222
BsmI GAATGC 3 cut(s) 10, 1127, 2292
BsnI GGCC 8 cut(s) 294, 374, 626, 1590, 1659, 1937, 2226, 2338
Bso31I GGTCTC 2 cut(s) 1920, 2433
BsoBI CYCGRG 2 cut(s) 124, 1153
Bsp13I TCCGGA 1 cut(s) 571
Bsp1407I TGTACA 2 cut(s) 597, 923
Bsp19I CCATGG 2 cut(s) 369, 1095
BspACI CCGC 8 cut(s) 418, 584, 603, 622, 908, 1169, 1581, 2506
BspANI GGCC 8 cut(s) 294, 374, 626, 1590, 1659, 1937, 2226, 2338
BspCNI CTCAG 2 cut(s) 1568, 1635
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 1 cut(s) 2037
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 8 cut(s) 422, 509, 544, 1062, 1101, 1102, 1591, 1718
BspMI ACCTGC 1 cut(s) 1151
BspPI GGATC 8 cut(s) 486, 530, 563, 637, 644, 822, 1472, 1889
BspQI GCTCTTC 1 cut(s) 2388
BspT107I GGYRCC 1 cut(s) 1060
BspTNI GGTCTC 2 cut(s) 1920, 2433
BsrDI GCAATG 4 cut(s) 32, 1869, 1948, 2082
BsrFI RCCGGY 4 cut(s) 486, 504, 2159, 2334
BsrGI TGTACA 2 cut(s) 597, 923
BsrI ACTGG 5 cut(s) 736, 839, 1956, 2149, 2445
BssAI RCCGGY 4 cut(s) 486, 504, 2159, 2334
BssECI CCNNGG 7 cut(s) 160, 369, 843, 935, 1095, 1660, 2232
BssT1I CCWWGG 3 cut(s) 369, 1095, 2232
Bst2UI CCWGG 5 cut(s) 162, 936, 1661, 1796, 2403
Bst4CI ACNGT 5 cut(s) 795, 893, 1487, 2114, 2289
Bst6I CTCTTC 8 cut(s) 358, 1110, 1385, 1665, 1749, 2313, 2388, 2411
BstAUI TGTACA 2 cut(s) 597, 923
BstC8I GCNNGC 5 cut(s) 624, 840, 1320, 1939, 2506
BstDEI CTNAG 6 cut(s) 948, 1301, 1523, 1555, 1643, 1732
BstDSI CCRYGG 2 cut(s) 369, 1095
BstEII GGTNACC 1 cut(s) 1163
BstF5I GGATG 8 cut(s) 580, 768, 916, 991, 995, 1010, 1190, 1917
BstFNI CGCG 1 cut(s) 2037
BstHHI GCGC 2 cut(s) 145, 1081
BstMAI GTCTC 5 cut(s) 127, 234, 1920, 2364, 2433
BstMWI GCNNNNNNNGC 5 cut(s) 142, 487, 496, 1283, 1587
BstNI CCWGG 5 cut(s) 162, 936, 1661, 1796, 2403
BstNSI RCATGY 7 cut(s) 842, 930, 1085, 1286, 2062, 2204, 2490
BstPI GGTNACC 1 cut(s) 1163
BstSCI CCNGG 9 cut(s) 160, 494, 538, 545, 843, 934, 1659, 1794, 2401
BstSFI CTRYAG 2 cut(s) 1536, 2110
BstUI CGCG 1 cut(s) 2037
BstV1I GCAGC 3 cut(s) 598, 2115, 2390
BstX2I RGATCY 3 cut(s) 629, 1477, 1881
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 3 cut(s) 629, 1477, 1881
BsuI GTATCC 3 cut(s) 379, 668, 1207
BsuRI GGCC 8 cut(s) 294, 374, 626, 1590, 1659, 1937, 2226, 2338
BtgI CCRYGG 2 cut(s) 369, 1095
BtsCI GGATG 8 cut(s) 580, 768, 916, 991, 995, 1010, 1190, 1917
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 2100
BveI ACCTGC 1 cut(s) 1151
Cac8I GCNNGC 5 cut(s) 624, 840, 1320, 1939, 2506
CaiI CAGNNNCTG 1 cut(s) 1481
CciI TCATGA 1 cut(s) 726
CfoI GCGC 2 cut(s) 145, 1081
Cfr10I RCCGGY 4 cut(s) 486, 504, 2159, 2334
Cfr13I GGNCC 6 cut(s) 624, 795, 878, 1100, 1377, 1589
CseI GACGC 1 cut(s) 1857
Csp6I GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2323, 2448, 2454
CspAI ACCGGT 2 cut(s) 504, 2159
CspCI CAANNNNNGTGG 2 cut(s) 2019, 2054
CviQI GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2323, 2448, 2454
DdeI CTNAG 6 cut(s) 948, 1301, 1523, 1555, 1643, 1732
DrdI GACNNNNNNGTC 1 cut(s) 248
DriI GACNNNNNGTC 1 cut(s) 1098
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 3 cut(s) 372, 1657, 2336
Eam1104I CTCTTC 8 cut(s) 358, 1110, 1385, 1665, 1749, 2313, 2388, 2411
Eam1105I GACNNNNNGTC 1 cut(s) 1098
EarI CTCTTC 8 cut(s) 358, 1110, 1385, 1665, 1749, 2313, 2388, 2411
EciI GGCGGA 2 cut(s) 433, 1596
Eco130I CCWWGG 3 cut(s) 369, 1095, 2232
Eco147I AGGCCT 1 cut(s) 294
Eco31I GGTCTC 2 cut(s) 1920, 2433
Eco47I GGWCC 4 cut(s) 795, 878, 1100, 1377
Eco57I CTGAAG 1 cut(s) 139
Eco88I CYCGRG 2 cut(s) 124, 1153
Eco91I GGTNACC 1 cut(s) 1163
EcoO109I RGGNCCY 1 cut(s) 1100
EcoO65I GGTNACC 1 cut(s) 1163
EcoRII CCWGG 5 cut(s) 160, 934, 1659, 1794, 2401
EcoT14I CCWWGG 3 cut(s) 369, 1095, 2232
EcoT22I ATGCAT 1 cut(s) 1127
ErhI CCWWGG 3 cut(s) 369, 1095, 2232
FaqI GGGAC 4 cut(s) 511, 1086, 1217, 2222
FauI CCCGC 2 cut(s) 615, 1176
FauNDI CATATG 2 cut(s) 2050, 2273
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 1 cut(s) 2109
Fnu4HI GCNGC 3 cut(s) 587, 2129, 2379
FokI GGATG 8 cut(s) 587, 775, 923, 997, 998, 1002, 1197, 1904
Fsp4HI GCNGC 3 cut(s) 587, 2129, 2379
FspI TGCGCA 1 cut(s) 1080
GlaI GCGC 2 cut(s) 144, 1080
GluI GCNGC 3 cut(s) 587, 2129, 2379
HaeIII GGCC 8 cut(s) 294, 374, 626, 1590, 1659, 1937, 2226, 2338
HgaI GACGC 1 cut(s) 1857
HhaI GCGC 2 cut(s) 145, 1081
Hin6I GCGC 2 cut(s) 143, 1079
HinP1I GCGC 2 cut(s) 143, 1079
HincII GTYRAC 1 cut(s) 2245
HindII GTYRAC 1 cut(s) 2245
HindIII AAGCTT 1 cut(s) 1568
HphI GGTGA 5 cut(s) 809, 1041, 1175, 2055, 2382
Hpy166II GTNNAC 5 cut(s) 703, 924, 1087, 2110, 2245
Hpy188I TCNGA 8 cut(s) 641, 985, 1504, 1558, 1727, 2006, 2104, 2173
Hpy8I GTNNAC 5 cut(s) 703, 924, 1087, 2110, 2245
HpyAV CCTTC 8 cut(s) 425, 1025, 1036, 1303, 1571, 2075, 2216, 2397
HpyCH4III ACNGT 5 cut(s) 795, 893, 1487, 2114, 2289
HpyCH4IV ACGT 2 cut(s) 178, 183
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 487, 496, 1283, 1587
HpyF3I CTNAG 6 cut(s) 948, 1301, 1523, 1555, 1643, 1732
HpySE526I ACGT 2 cut(s) 178, 183
HspAI GCGC 2 cut(s) 143, 1079
KflI GGGWCCC 1 cut(s) 1100
Kpn2I TCCGGA 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 24
LguI GCTCTTC 1 cut(s) 2388
LmnI GCTCC 5 cut(s) 420, 616, 960, 1007, 2006
Lsp1109I GCAGC 3 cut(s) 598, 2115, 2390
LweI GCATC 5 cut(s) 1019, 1175, 1752, 2365, 2452
MaeII ACGT 2 cut(s) 178, 183
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 3 cut(s) 629, 1477, 1881
MlsI TGGCCA 2 cut(s) 374, 1659
MluNI TGGCCA 2 cut(s) 374, 1659
MlyI GAGTC 5 cut(s) 664, 740, 1061, 1793, 2177
MmeI TCCRAC 5 cut(s) 166, 363, 1482, 2023, 2176
Mox20I TGGCCA 2 cut(s) 374, 1659
Mph1103I ATGCAT 1 cut(s) 1127
MroI TCCGGA 1 cut(s) 571
MroXI GAANNNNTTC 1 cut(s) 506
MscI TGGCCA 2 cut(s) 374, 1659
MseI TTAA 2 cut(s) 738, 1910
MslI CAYNNNNRTG 3 cut(s) 1015, 1385, 2308
Msp20I TGGCCA 2 cut(s) 374, 1659
MspA1I CMGCKG 2 cut(s) 589, 603
MspR9I CCNGG 9 cut(s) 162, 496, 540, 547, 845, 936, 1661, 1796, 2403
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 3 cut(s) 10, 1127, 2292
MvaI CCWGG 5 cut(s) 162, 936, 1661, 1796, 2403
MvnI CGCG 1 cut(s) 2037
MwoI GCNNNNNNNGC 5 cut(s) 142, 487, 496, 1283, 1587
NciI CCSGG 4 cut(s) 496, 540, 547, 845
NcoI CCATGG 2 cut(s) 369, 1095
NdeI CATATG 2 cut(s) 2050, 2273
NlaIV GGNNCC 8 cut(s) 422, 509, 544, 1062, 1101, 1102, 1591, 1718
NmeAIII GCCGAG 1 cut(s) 2252
NmuCI GTSAC 6 cut(s) 857, 893, 1047, 1163, 1189, 2061
NsbI TGCGCA 1 cut(s) 1080
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 7 cut(s) 842, 930, 1085, 1286, 2062, 2204, 2490
OliI CACNNNNGTG 1 cut(s) 2308
PaeI GCATGC 1 cut(s) 842
PaeR7I CTCGAG 2 cut(s) 124, 1153
PagI TCATGA 1 cut(s) 726
PaqCI CACCTGC 1 cut(s) 1151
PceI AGGCCT 1 cut(s) 294
PciI ACATGT 2 cut(s) 2058, 2200
PciSI GCTCTTC 1 cut(s) 2388
PctI GAATGC 3 cut(s) 10, 1127, 2292
PdmI GAANNNNTTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 225
PfoI TCCNGGA 1 cut(s) 538
PinAI ACCGGT 2 cut(s) 504, 2159
PkrI GCNGC 3 cut(s) 588, 2130, 2380
PleI GAGTC 5 cut(s) 664, 740, 1061, 1793, 2177
PpsI GAGTC 5 cut(s) 664, 740, 1061, 1793, 2177
PpuMI RGGWCCY 1 cut(s) 1100
PscI ACATGT 2 cut(s) 2058, 2200
PsiI TTATAA 1 cut(s) 1599
Psp5II RGGWCCY 1 cut(s) 1100
Psp6I CCWGG 5 cut(s) 160, 934, 1659, 1794, 2401
PspEI GGTNACC 1 cut(s) 1163
PspGI CCWGG 5 cut(s) 160, 934, 1659, 1794, 2401
PspN4I GGNNCC 8 cut(s) 422, 509, 544, 1062, 1101, 1102, 1591, 1718
PspPI GGNCC 6 cut(s) 624, 795, 878, 1100, 1377, 1589
PspPPI RGGWCCY 1 cut(s) 1100
PspXI VCTCGAGB 1 cut(s) 1153
PsrI GAACNNNNNNTAC 2 cut(s) 2147, 2179
PstNI CAGNNNCTG 1 cut(s) 1481
PsuI RGATCY 3 cut(s) 629, 1477, 1881
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 8 cut(s) 599, 833, 925, 968, 1199, 2324, 2449, 2455
RsaNI GTAC 8 cut(s) 598, 832, 924, 967, 1198, 2323, 2448, 2454
RseI CAYNNNNRTG 3 cut(s) 1015, 1385, 2308
SapI GCTCTTC 1 cut(s) 2388
SaqAI TTAA 2 cut(s) 738, 1910
SatI GCNGC 3 cut(s) 587, 2129, 2379
Sau96I GGNCC 6 cut(s) 624, 795, 878, 1100, 1377, 1589
ScaI AGTACT 4 cut(s) 833, 2324, 2449, 2455
SchI GAGTC 5 cut(s) 664, 740, 1061, 1793, 2177
ScrFI CCNGG 9 cut(s) 162, 496, 540, 547, 845, 936, 1661, 1796, 2403
SfaNI GCATC 5 cut(s) 1019, 1175, 1752, 2365, 2452
SfcI CTRYAG 2 cut(s) 1536, 2110
Sfr274I CTCGAG 2 cut(s) 124, 1153
SinI GGWCC 4 cut(s) 795, 878, 1100, 1377
SlaI CTCGAG 2 cut(s) 124, 1153
SmiMI CAYNNNNRTG 3 cut(s) 1015, 1385, 2308
SmlI CTYRAG 4 cut(s) 124, 757, 970, 1153
SmoI CTYRAG 4 cut(s) 124, 757, 970, 1153
SpeI ACTAGT 2 cut(s) 692, 2450
SphI GCATGC 1 cut(s) 842
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 8 cut(s) 418, 584, 603, 622, 908, 1169, 1581, 2506
SspI AATATT 1 cut(s) 2001
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 9 cut(s) 160, 494, 538, 545, 843, 934, 1659, 1794, 2401
StyI CCWWGG 3 cut(s) 369, 1095, 2232
TaaI ACNGT 5 cut(s) 795, 893, 1487, 2114, 2289
TaiI ACGT 2 cut(s) 181, 186
TaqI TCGA 7 cut(s) 125, 279, 789, 918, 1154, 1255, 1904
TatI WGTACW 6 cut(s) 597, 831, 923, 2322, 2447, 2453
Tru1I TTAA 2 cut(s) 738, 1910
Tru9I TTAA 2 cut(s) 738, 1910
TscAI CASTG 2 cut(s) 34, 2100
TseFI GTSAC 6 cut(s) 857, 893, 1047, 1163, 1189, 2061
TseI GCWGC 3 cut(s) 586, 2128, 2378
Tsp45I GTSAC 6 cut(s) 857, 893, 1047, 1163, 1189, 2061
TspGWI ACGGA 6 cut(s) 330, 712, 882, 947, 1003, 1041
TspRI CASTG 2 cut(s) 34, 2100
Van91I CCANNNNNTGG 1 cut(s) 225
VpaK11BI GGWCC 4 cut(s) 795, 878, 1100, 1377
XapI RAATTY 5 cut(s) 166, 550, 1549, 1651, 2188
XceI RCATGY 7 cut(s) 842, 930, 1085, 1286, 2062, 2204, 2490
XhoI CTCGAG 2 cut(s) 124, 1153
XmiI GTMKAC 1 cut(s) 2109
XmnI GAANNNNTTC 1 cut(s) 506
ZrmI AGTACT 4 cut(s) 833, 2324, 2449, 2455
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.