G-type lectin S-receptor-like serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 908, 2338 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1196 |
| AciI | CCGC | 4 cut(s) | 484, 616, 1530, 2306 |
| AclWI | GGATC | 2 cut(s) | 863, 2398 |
| AcoI | YGGCCR | 1 cut(s) | 2264 |
| AcsI | RAATTY | 5 cut(s) | 189, 724, 1007, 1947, 2140 |
| AcuI | CTGAAG | 1 cut(s) | 2298 |
| AcvI | CACGTG | 1 cut(s) | 774 |
| AfaI | GTAC | 7 cut(s) | 593, 655, 898, 941, 1215, 1557, 1727 |
| AfeI | AGCGCT | 1 cut(s) | 1314 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 539, 649, 1196, 1739 |
| AflIII | ACRYGT | 2 cut(s) | 989, 2414 |
| AgsI | TTSAA | 6 cut(s) | 354, 917, 1405, 1697, 1854, 2225 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 484, 1605 |
| AhlI | ACTAGT | 1 cut(s) | 1297 |
| AjnI | CCWGG | 1 cut(s) | 532 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 458, 490 |
| Alw21I | GWGCWC | 4 cut(s) | 607, 790, 1451, 1780 |
| Alw26I | GTCTC | 1 cut(s) | 112 |
| AlwI | GGATC | 2 cut(s) | 863, 2398 |
| Ama87I | CYCGRG | 3 cut(s) | 392, 943, 1603 |
| Aor51HI | AGCGCT | 1 cut(s) | 1314 |
| AoxI | GGCC | 4 cut(s) | 277, 1741, 1884, 2264 |
| ApeKI | GCWGC | 5 cut(s) | 878, 1131, 1186, 1484, 1487 |
| ApoI | RAATTY | 5 cut(s) | 189, 724, 1007, 1947, 2140 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 500, 2038 |
| AspLEI | GCGC | 2 cut(s) | 1186, 1315 |
| AspS9I | GGNCC | 1 cut(s) | 851 |
| AsuC2I | CCSGG | 3 cut(s) | 541, 909, 2095 |
| AsuHPI | GGTGA | 5 cut(s) | 107, 2004, 2056, 2198, 2310 |
| AvaI | CYCGRG | 3 cut(s) | 392, 943, 1603 |
| AvaII | GGWCC | 1 cut(s) | 851 |
| BaeGI | GKGCMC | 1 cut(s) | 1054 |
| BanII | GRGCYC | 2 cut(s) | 607, 1451 |
| BbrPI | CACGTG | 1 cut(s) | 774 |
| BbsI | GAAGAC | 2 cut(s) | 1381, 1604 |
| Bbv12I | GWGCWC | 4 cut(s) | 607, 790, 1451, 1780 |
| BbvI | GCAGC | 5 cut(s) | 865, 1143, 1173, 1496, 1499 |
| BccI | CCATC | 7 cut(s) | 92, 266, 593, 944, 953, 1165, 1559 |
| BciT130I | CCWGG | 1 cut(s) | 534 |
| BciVI | GTATCC | 3 cut(s) | 364, 700, 2262 |
| BcnI | CCSGG | 3 cut(s) | 541, 909, 2095 |
| BcoDI | GTCTC | 1 cut(s) | 112 |
| BcuI | ACTAGT | 1 cut(s) | 1297 |
| BfaI | CTAG | 8 cut(s) | 156, 281, 567, 1047, 1298, 1824, 1944, 2294 |
| BfmI | CTRYAG | 1 cut(s) | 888 |
| BfoI | RGCGCY | 2 cut(s) | 1187, 1316 |
| BfuAI | ACCTGC | 2 cut(s) | 908, 2338 |
| BfuI | GTATCC | 3 cut(s) | 364, 700, 2262 |
| BglII | AGATCT | 1 cut(s) | 1876 |
| BisI | GCNGC | 6 cut(s) | 879, 1132, 1187, 1485, 1488, 2307 |
| BlsI | GCNGC | 6 cut(s) | 880, 1133, 1188, 1486, 1489, 2308 |
| BmcAI | AGTACT | 1 cut(s) | 1727 |
| Bme1390I | CCNGG | 4 cut(s) | 534, 541, 909, 2095 |
| Bme18I | GGWCC | 1 cut(s) | 851 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 484, 1605 |
| BmeT110I | CYCGRG | 3 cut(s) | 392, 943, 1603 |
| BmgT120I | GGNCC | 1 cut(s) | 851 |
| BmiI | GGNNCC | 3 cut(s) | 538, 1428, 1540 |
| BmrFI | CCNGG | 4 cut(s) | 534, 541, 909, 2095 |
| BmsI | GCATC | 4 cut(s) | 638, 1213, 1701, 1894 |
| BpiI | GAAGAC | 2 cut(s) | 1381, 1604 |
| BpmI | CTGGAG | 1 cut(s) | 270 |
| BpuEI | CTTGAG | 1 cut(s) | 715 |
| BpuMI | CCSGG | 3 cut(s) | 541, 909, 2095 |
| BsaAI | YACGTR | 1 cut(s) | 774 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1715 |
| BsaJI | CCNNGG | 5 cut(s) | 532, 608, 908, 1602, 1813 |
| BsaWI | WCCGGW | 1 cut(s) | 2014 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 267, 297 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 539, 649, 1196, 1739 |
| Bse118I | RCCGGY | 1 cut(s) | 2262 |
| Bse1I | ACTGG | 3 cut(s) | 1147, 1201, 1420 |
| Bse3DI | GCAATG | 2 cut(s) | 1897, 2031 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1715 |
| BseBI | CCWGG | 1 cut(s) | 534 |
| BseDI | CCNNGG | 5 cut(s) | 532, 608, 908, 1602, 1813 |
| BseGI | GGATG | 5 cut(s) | 604, 964, 1151, 1359, 2128 |
| BseJI | GATNNNNATC | 1 cut(s) | 1715 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 539, 649, 1196, 1739 |
| BseMI | GCAATG | 2 cut(s) | 1897, 2031 |
| BseMII | CTCAG | 2 cut(s) | 1340, 1464 |
| BseNI | ACTGG | 3 cut(s) | 1147, 1201, 1420 |
| BseSI | GKGCMC | 1 cut(s) | 1054 |
| BseXI | GCAGC | 5 cut(s) | 865, 1143, 1173, 1496, 1499 |
| BsgI | GTGCAG | 1 cut(s) | 165 |
| BshFI | GGCC | 4 cut(s) | 279, 1743, 1886, 2266 |
| BsiHKAI | GWGCWC | 4 cut(s) | 607, 790, 1451, 1780 |
| BsiHKCI | CYCGRG | 3 cut(s) | 392, 943, 1603 |
| BsiSI | CCGG | 6 cut(s) | 111, 540, 908, 2015, 2094, 2263 |
| BslFI | GGGAC | 2 cut(s) | 221, 967 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 539, 649, 1196, 1739 |
| BsmAI | GTCTC | 1 cut(s) | 112 |
| BsmFI | GGGAC | 2 cut(s) | 221, 967 |
| BsmI | GAATGC | 2 cut(s) | 1100, 2333 |
| BsnI | GGCC | 4 cut(s) | 279, 1743, 1886, 2266 |
| BsoBI | CYCGRG | 3 cut(s) | 392, 943, 1603 |
| Bsp1286I | GDGCHC | 5 cut(s) | 607, 790, 1054, 1451, 1780 |
| Bsp1407I | TGTACA | 1 cut(s) | 591 |
| Bsp143I | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| Bsp19I | CCATGG | 1 cut(s) | 608 |
| BspACI | CCGC | 4 cut(s) | 484, 616, 1530, 2306 |
| BspANI | GGCC | 4 cut(s) | 279, 1743, 1886, 2266 |
| BspCNI | CTCAG | 2 cut(s) | 1341, 1463 |
| BspLI | GGNNCC | 3 cut(s) | 538, 1428, 1540 |
| BspMI | ACCTGC | 2 cut(s) | 908, 2338 |
| BspPI | GGATC | 2 cut(s) | 863, 2398 |
| BspQI | GCTCTTC | 1 cut(s) | 2316 |
| BsrDI | GCAATG | 2 cut(s) | 1897, 2031 |
| BsrFI | RCCGGY | 1 cut(s) | 2262 |
| BsrGI | TGTACA | 1 cut(s) | 591 |
| BsrI | ACTGG | 3 cut(s) | 1147, 1201, 1420 |
| BssAI | RCCGGY | 1 cut(s) | 2262 |
| BssECI | CCNNGG | 5 cut(s) | 532, 608, 908, 1602, 1813 |
| BssMI | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| BssT1I | CCWWGG | 1 cut(s) | 608 |
| Bst2UI | CCWGG | 1 cut(s) | 534 |
| Bst4CI | ACNGT | 7 cut(s) | 95, 164, 205, 350, 739, 768, 1127 |
| Bst6I | CTCTTC | 4 cut(s) | 343, 1352, 1698, 2316 |
| BstAUI | TGTACA | 1 cut(s) | 591 |
| BstBAI | YACGTR | 1 cut(s) | 774 |
| BstC8I | GCNNGC | 7 cut(s) | 148, 618, 1084, 1088, 1287, 1888, 2243 |
| BstDEI | CTNAG | 4 cut(s) | 708, 1268, 1349, 1450 |
| BstDSI | CCRYGG | 2 cut(s) | 608, 1813 |
| BstF5I | GGATG | 5 cut(s) | 604, 964, 1151, 1359, 2128 |
| BstH2I | RGCGCY | 2 cut(s) | 1187, 1316 |
| BstHHI | GCGC | 2 cut(s) | 1186, 1315 |
| BstKTI | GATC | 5 cut(s) | 784, 871, 1879, 1963, 2393 |
| BstMAI | GTCTC | 1 cut(s) | 112 |
| BstMBI | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 490, 626, 1244, 1286, 1536 |
| BstNI | CCWGG | 1 cut(s) | 534 |
| BstNSI | RCATGY | 2 cut(s) | 993, 2418 |
| BstSCI | CCNGG | 4 cut(s) | 532, 539, 907, 2093 |
| BstSFI | CTRYAG | 1 cut(s) | 888 |
| BstSLI | GKGCMC | 1 cut(s) | 1054 |
| BstV1I | GCAGC | 5 cut(s) | 865, 1143, 1173, 1496, 1499 |
| BstV2I | GAAGAC | 2 cut(s) | 1381, 1604 |
| BstX2I | RGATCY | 2 cut(s) | 1876, 2390 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 367, 936 |
| BstYI | RGATCY | 2 cut(s) | 1876, 2390 |
| BsuI | GTATCC | 3 cut(s) | 364, 700, 2262 |
| BsuRI | GGCC | 4 cut(s) | 279, 1743, 1886, 2266 |
| BtgI | CCRYGG | 2 cut(s) | 608, 1813 |
| BtgZI | GCGATG | 1 cut(s) | 1646 |
| BtsCI | GGATG | 5 cut(s) | 604, 964, 1151, 1359, 2128 |
| BtsI | GCAGTG | 1 cut(s) | 1587 |
| BtsIMutI | CAGTG | 3 cut(s) | 148, 441, 1587 |
| BveI | ACCTGC | 2 cut(s) | 908, 2338 |
| Cac8I | GCNNGC | 7 cut(s) | 148, 618, 1084, 1088, 1287, 1888, 2243 |
| CfoI | GCGC | 2 cut(s) | 1186, 1315 |
| Cfr10I | RCCGGY | 1 cut(s) | 2262 |
| Cfr13I | GGNCC | 1 cut(s) | 851 |
| CseI | GACGC | 1 cut(s) | 1806 |
| Csp6I | GTAC | 7 cut(s) | 592, 654, 897, 940, 1214, 1556, 1726 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1968, 2003 |
| CviAII | CATG | 9 cut(s) | 609, 648, 990, 1155, 1444, 2120, 2272, 2290, 2415 |
| CviQI | GTAC | 7 cut(s) | 592, 654, 897, 940, 1214, 1556, 1726 |
| DdeI | CTNAG | 4 cut(s) | 708, 1268, 1349, 1450 |
| DpnI | GATC | 5 cut(s) | 783, 870, 1878, 1962, 2392 |
| DpnII | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| DraI | TTTAAA | 1 cut(s) | 1627 |
| DriI | GACNNNNNGTC | 2 cut(s) | 484, 1605 |
| EaeI | YGGCCR | 1 cut(s) | 2264 |
| Eam1104I | CTCTTC | 4 cut(s) | 343, 1352, 1698, 2316 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 484, 1605 |
| EarI | CTCTTC | 4 cut(s) | 343, 1352, 1698, 2316 |
| Ecl136II | GAGCTC | 2 cut(s) | 605, 1449 |
| Eco130I | CCWWGG | 1 cut(s) | 608 |
| Eco147I | AGGCCT | 2 cut(s) | 279, 1743 |
| Eco24I | GRGCYC | 2 cut(s) | 607, 1451 |
| Eco47I | GGWCC | 1 cut(s) | 851 |
| Eco47III | AGCGCT | 1 cut(s) | 1314 |
| Eco53kI | GAGCTC | 2 cut(s) | 605, 1449 |
| Eco57I | CTGAAG | 1 cut(s) | 2298 |
| Eco72I | CACGTG | 1 cut(s) | 774 |
| Eco88I | CYCGRG | 3 cut(s) | 392, 943, 1603 |
| EcoICRI | GAGCTC | 2 cut(s) | 605, 1449 |
| EcoRII | CCWGG | 1 cut(s) | 532 |
| EcoT14I | CCWWGG | 1 cut(s) | 608 |
| EcoT38I | GRGCYC | 2 cut(s) | 607, 1451 |
| ErhI | CCWWGG | 1 cut(s) | 608 |
| FaeI | CATG | 9 cut(s) | 612, 651, 993, 1158, 1447, 2123, 2275, 2293, 2418 |
| FaqI | GGGAC | 2 cut(s) | 221, 967 |
| FatI | CATG | 9 cut(s) | 608, 647, 989, 1154, 1443, 2119, 2271, 2289, 2414 |
| FauI | CCCGC | 1 cut(s) | 609 |
| FauNDI | CATATG | 1 cut(s) | 1999 |
| Fnu4HI | GCNGC | 6 cut(s) | 879, 1132, 1187, 1485, 1488, 2307 |
| FokI | GGATG | 5 cut(s) | 611, 971, 1158, 1366, 2115 |
| FriOI | GRGCYC | 2 cut(s) | 607, 1451 |
| Fsp4HI | GCNGC | 6 cut(s) | 879, 1132, 1187, 1485, 1488, 2307 |
| FspBI | CTAG | 8 cut(s) | 156, 281, 567, 1047, 1298, 1824, 1944, 2294 |
| GlaI | GCGC | 2 cut(s) | 1185, 1314 |
| GluI | GCNGC | 6 cut(s) | 879, 1132, 1187, 1485, 1488, 2307 |
| GsuI | CTGGAG | 1 cut(s) | 270 |
| HaeII | RGCGCY | 2 cut(s) | 1187, 1316 |
| HaeIII | GGCC | 4 cut(s) | 279, 1743, 1886, 2266 |
| HapII | CCGG | 6 cut(s) | 111, 540, 908, 2015, 2094, 2263 |
| HgaI | GACGC | 1 cut(s) | 1806 |
| HhaI | GCGC | 2 cut(s) | 1186, 1315 |
| Hin1II | CATG | 9 cut(s) | 612, 651, 993, 1158, 1447, 2123, 2275, 2293, 2418 |
| Hin6I | GCGC | 2 cut(s) | 1184, 1313 |
| HinP1I | GCGC | 2 cut(s) | 1184, 1313 |
| HincII | GTYRAC | 2 cut(s) | 640, 2379 |
| HindII | GTYRAC | 2 cut(s) | 640, 2379 |
| HindIII | AAGCTT | 2 cut(s) | 362, 1517 |
| HinfI | GANTC | 7 cut(s) | 749, 1262, 1409, 1850, 2135, 2174, 2189 |
| HpaII | CCGG | 6 cut(s) | 111, 540, 908, 2015, 2094, 2263 |
| HphI | GGTGA | 5 cut(s) | 107, 2004, 2056, 2198, 2310 |
| Hpy166II | GTNNAC | 5 cut(s) | 640, 673, 771, 1556, 2379 |
| Hpy188I | TCNGA | 3 cut(s) | 1453, 2053, 2278 |
| Hpy8I | GTNNAC | 5 cut(s) | 640, 673, 771, 1556, 2379 |
| HpyAV | CCTTC | 8 cut(s) | 914, 1270, 1411, 2028, 2144, 2242, 2325, 2381 |
| HpyCH4III | ACNGT | 7 cut(s) | 95, 164, 205, 350, 739, 768, 1127 |
| HpyCH4IV | ACGT | 2 cut(s) | 168, 773 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 490, 626, 1244, 1286, 1536 |
| HpyF3I | CTNAG | 4 cut(s) | 708, 1268, 1349, 1450 |
| HpySE526I | ACGT | 2 cut(s) | 168, 773 |
| Hsp92II | CATG | 9 cut(s) | 612, 651, 993, 1158, 1447, 2123, 2275, 2293, 2418 |
| HspAI | GCGC | 2 cut(s) | 1184, 1313 |
| Kzo9I | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| LguI | GCTCTTC | 1 cut(s) | 2316 |
| LmnI | GCTCC | 6 cut(s) | 543, 602, 610, 820, 1403, 1544 |
| Lsp1109I | GCAGC | 5 cut(s) | 865, 1143, 1173, 1496, 1499 |
| LweI | GCATC | 4 cut(s) | 638, 1213, 1701, 1894 |
| MaeI | CTAG | 8 cut(s) | 156, 281, 567, 1047, 1298, 1824, 1944, 2294 |
| MaeII | ACGT | 2 cut(s) | 168, 773 |
| MaeIII | GTNAC | 8 cut(s) | 158, 164, 328, 860, 1150, 1344, 2010, 2297 |
| MalI | GATC | 5 cut(s) | 783, 870, 1878, 1962, 2392 |
| MboI | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| MfeI | CAATTG | 1 cut(s) | 354 |
| MflI | RGATCY | 2 cut(s) | 1876, 2390 |
| MhlI | GDGCHC | 5 cut(s) | 607, 790, 1054, 1451, 1780 |
| MlyI | GAGTC | 1 cut(s) | 2129 |
| MmeI | TCCRAC | 3 cut(s) | 151, 1681, 1972 |
| MroXI | GAANNNNTTC | 2 cut(s) | 500, 2038 |
| MseI | TTAA | 8 cut(s) | 506, 1059, 1617, 1626, 1761, 1859, 1953, 2144 |
| MslI | CAYNNNNRTG | 1 cut(s) | 793 |
| MspA1I | CMGCKG | 1 cut(s) | 2258 |
| MspI | CCGG | 6 cut(s) | 111, 540, 908, 2015, 2094, 2263 |
| MspR9I | CCNGG | 4 cut(s) | 534, 541, 909, 2095 |
| MunI | CAATTG | 1 cut(s) | 354 |
| Mva1269I | GAATGC | 2 cut(s) | 1100, 2333 |
| MvaI | CCWGG | 1 cut(s) | 534 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 490, 626, 1244, 1286, 1536 |
| NciI | CCSGG | 3 cut(s) | 541, 909, 2095 |
| NcoI | CCATGG | 1 cut(s) | 608 |
| NdeI | CATATG | 1 cut(s) | 1999 |
| NdeII | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| NlaIII | CATG | 9 cut(s) | 612, 651, 993, 1158, 1447, 2123, 2275, 2293, 2418 |
| NlaIV | GGNNCC | 3 cut(s) | 538, 1428, 1540 |
| NmuCI | GTSAC | 3 cut(s) | 328, 1150, 2010 |
| NspI | RCATGY | 2 cut(s) | 993, 2418 |
| PaeR7I | CTCGAG | 2 cut(s) | 392, 943 |
| PceI | AGGCCT | 2 cut(s) | 279, 1743 |
| PciI | ACATGT | 2 cut(s) | 989, 2414 |
| PciSI | GCTCTTC | 1 cut(s) | 2316 |
| PctI | GAATGC | 2 cut(s) | 1100, 2333 |
| PdmI | GAANNNNTTC | 2 cut(s) | 500, 2038 |
| PfeI | GAWTC | 6 cut(s) | 749, 1262, 1409, 1850, 2174, 2189 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1196 |
| PkrI | GCNGC | 6 cut(s) | 880, 1133, 1188, 1486, 1489, 2308 |
| PleI | GAGTC | 1 cut(s) | 2129 |
| PmaCI | CACGTG | 1 cut(s) | 774 |
| PmlI | CACGTG | 1 cut(s) | 774 |
| PpsI | GAGTC | 1 cut(s) | 2129 |
| Ppu21I | YACGTR | 1 cut(s) | 774 |
| PscI | ACATGT | 2 cut(s) | 989, 2414 |
| Psp124BI | GAGCTC | 2 cut(s) | 607, 1451 |
| Psp6I | CCWGG | 1 cut(s) | 532 |
| PspCI | CACGTG | 1 cut(s) | 774 |
| PspGI | CCWGG | 1 cut(s) | 532 |
| PspN4I | GGNNCC | 3 cut(s) | 538, 1428, 1540 |
| PspPI | GGNCC | 1 cut(s) | 851 |
| PsuI | RGATCY | 2 cut(s) | 1876, 2390 |
| PvuII | CAGCTG | 1 cut(s) | 2258 |
| RsaI | GTAC | 7 cut(s) | 593, 655, 898, 941, 1215, 1557, 1727 |
| RsaNI | GTAC | 7 cut(s) | 592, 654, 897, 940, 1214, 1556, 1726 |
| RseI | CAYNNNNRTG | 1 cut(s) | 793 |
| SacI | GAGCTC | 2 cut(s) | 607, 1451 |
| SapI | GCTCTTC | 1 cut(s) | 2316 |
| SaqAI | TTAA | 8 cut(s) | 506, 1059, 1617, 1626, 1761, 1859, 1953, 2144 |
| SatI | GCNGC | 6 cut(s) | 879, 1132, 1187, 1485, 1488, 2307 |
| Sau3AI | GATC | 5 cut(s) | 781, 868, 1876, 1960, 2390 |
| Sau96I | GGNCC | 1 cut(s) | 851 |
| ScaI | AGTACT | 1 cut(s) | 1727 |
| SchI | GAGTC | 1 cut(s) | 2129 |
| ScrFI | CCNGG | 4 cut(s) | 534, 541, 909, 2095 |
| SduI | GDGCHC | 5 cut(s) | 607, 790, 1054, 1451, 1780 |
| SfaNI | GCATC | 4 cut(s) | 638, 1213, 1701, 1894 |
| SfcI | CTRYAG | 1 cut(s) | 888 |
| Sfr274I | CTCGAG | 2 cut(s) | 392, 943 |
| SinI | GGWCC | 1 cut(s) | 851 |
| SlaI | CTCGAG | 2 cut(s) | 392, 943 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 793 |
| SmlI | CTYRAG | 3 cut(s) | 392, 730, 943 |
| SmoI | CTYRAG | 3 cut(s) | 392, 730, 943 |
| SpeI | ACTAGT | 1 cut(s) | 1297 |
| SseBI | AGGCCT | 2 cut(s) | 279, 1743 |
| SsiI | CCGC | 4 cut(s) | 484, 616, 1530, 2306 |
| SspI | AATATT | 3 cut(s) | 18, 806, 1388 |
| SspMI | CTAG | 8 cut(s) | 156, 281, 567, 1047, 1298, 1824, 1944, 2294 |
| SstI | GAGCTC | 2 cut(s) | 607, 1451 |
| StuI | AGGCCT | 2 cut(s) | 279, 1743 |
| StyD4I | CCNGG | 4 cut(s) | 532, 539, 907, 2093 |
| StyI | CCWWGG | 1 cut(s) | 608 |
| TaaI | ACNGT | 7 cut(s) | 95, 164, 205, 350, 739, 768, 1127 |
| TaiI | ACGT | 2 cut(s) | 171, 776 |
| TaqI | TCGA | 8 cut(s) | 393, 521, 556, 784, 944, 994, 1595, 1746 |
| TatI | WGTACW | 3 cut(s) | 591, 1555, 1725 |
| TauI | GCSGC | 1 cut(s) | 2309 |
| TfiI | GAWTC | 6 cut(s) | 749, 1262, 1409, 1850, 2174, 2189 |
| Tru1I | TTAA | 8 cut(s) | 506, 1059, 1617, 1626, 1761, 1859, 1953, 2144 |
| Tru9I | TTAA | 8 cut(s) | 506, 1059, 1617, 1626, 1761, 1859, 1953, 2144 |
| TscAI | CASTG | 3 cut(s) | 148, 448, 1587 |
| TseFI | GTSAC | 3 cut(s) | 328, 1150, 2010 |
| TseI | GCWGC | 5 cut(s) | 878, 1131, 1186, 1484, 1487 |
| Tsp45I | GTSAC | 3 cut(s) | 328, 1150, 2010 |
| TspGWI | ACGGA | 3 cut(s) | 971, 1531, 1830 |
| TspRI | CASTG | 3 cut(s) | 148, 448, 1587 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1196 |
| VpaK11BI | GGWCC | 1 cut(s) | 851 |
| XapI | RAATTY | 5 cut(s) | 189, 724, 1007, 1947, 2140 |
| XceI | RCATGY | 2 cut(s) | 993, 2418 |
| XhoI | CTCGAG | 2 cut(s) | 392, 943 |
| XmnI | GAANNNNTTC | 2 cut(s) | 500, 2038 |
| XspI | CTAG | 8 cut(s) | 156, 281, 567, 1047, 1298, 1824, 1944, 2294 |
| ZrmI | AGTACT | 1 cut(s) | 1727 |