FvH4_3g03243

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000099
Physical Location & Seq
Reverse (-)
1754164 .. 1757586
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03243.t1

Sequence Viewer

Length: 2523 bp
ATGCACTCTGAAAAAGGTTTACTGAATGCATTACTTCAGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCAGTTGTGCAATTGTTCTACCATGAACATCACTTTCAACCAATCAATCAGAGATGGTGACTTGATGCTCTCCAACGACGAGACCTTTGTACTTGGATTCTTCAGCCCTGACACATCCAGAAAACACTATGTTGGAGTCTGGTACAAGTTCTCAGAAGACACGGTTGTGTGGGTAGCTAACAGAGACGATCCTGTCAATGCTAACTCGGGAATTCTCACAATTAGTACTGATGGAAATCTCGTTTTAACGCATGGTGATAGCCAAGGCCTTCCTCTGTGGTCATCAAATGTTTCTGTCTCACCAAGTAACACTAATACCATGGCACAACTCCTGGATTCAGGAAACCTGGTTTTGGTTAATCAAGACACCAACCACAGTAATGTCTTATGGCAGAGCTTCAATCATCCTACACATGTTCTTCTTCCAAATATGAAAATTGGAGTGGACCATGGTAAGAACATGTACCTCACATCATGGAATTCAAACAATGATCCTGGAACGGGGAATTGCTCATTAAAGACAGAACTGTATGGATCCCCTCAGATAATATTGTACAACAATCATGCTAAATGGTGGCGGTTAAGTGAACGTGATGCAGTTTTTGTGAGTGATAAAATAAGCAGGCAAAGCAGCAATGGTACATTTGACATAATTCTTGTGAACAATGAAGATGAGGTTTCTACGACATGGGCTGTTCTTGACCATTCGGTTCACTCAATAATAGTGATATCTGGGTCTGGTACATCCGGATCAGTTCAACAGCTAGCATGGCAGGGAGAGCAGCAGGCGTGGGTAGTCGTTTGGTCAGCACCAACAGATGTCTGTGACAAGTATGGTAAGTGTGGCCCTTTTGGAATTTGCAATATGCACACGGCTAACTGGGTGAAATGTGCATGCTTTCCTGGATATGAACCCAACTTCCCACAGGATTGGAAATTAAGAGAAGAGTCAGGTGGGTGCAACAGGCAACACGGGGCTCCATCCATGTGTAGGAACGGTGAGGGGTTCGTAAGGATGGAAAATGTAAATATTCCAGATACTTCCAATGTCGAATTGGACAAGAATATGAGTCTGGAAGCATGTGAGCAGAAGTGTTTGAATAACTGCTCTTGCCTGGCATATGCCAGTGCAGATATCAGGAATGGAAGTGGCTGTATGACCTGGTATGGAGATTTAATGGATACTAAGCAGTATATTGAACGACAAGGACAAGACTTATACATACGTGCCAATGCAATAGTAGCAGCTAAATATGCAAAGAAGTCAAAGAGGTACCTTGCCAAAGAGGGGGTGTTCTTGATTTTGGTATTACCAACTATTGCAATGATATTTATTATATTTTCTACACGTTGGTGTTCAAGGAGGAAGACAAAAAGATCTCTGAGGCAATTCAGGCAAATGAAGCATGATGACAGTAAAATTCCACTGACCTGGCATGATGGGGAGATGAACCAAGACCTGGCATTTTTTGACCTAAACAGCATAGTTGTTGCCACAGACAACTTTTCCATTAGTAACAAGCTTGGCGAAGGTGGTTTTGGCTCAGTCTATAAGGGGCTCCTAGCTAATGGACAAGAGGTTGCTGTGAAAAGATTATCAATGGATTCTAGGCAAGGTTTAGAACAATTTAAAAATGAGGTTATGGTGATAGCAAACCTTCAGCACAGGAACCTGGTGAGGCTTTATGGTTGCTGCATCGACTCAGAAGAGAAGATGCTGATCTATGAGTACCTACCAAACAAAAGCTTGGACTTCTTCATATTTGGAAAAAGTAGAAGCTTATCAATGGACTGGAAACAGCGTTTCAATATTATTAATGGAATTTCAAGAGGGGTCTTATATCTTCATCAAGACTCGAGACTAAAAATTATCCATAGGGACATAAAAGCTAGCAATGTCCTATTGGATTCTGCAATGAACCCCAATATCTCAGATTTTGGACTCGCTAAAATGTTTGGGGAAGACCAAATCCAAGCTAATACAAACAGAGTGGTCGGAACATATGGCTACATGTCACCAGAATATGCAATGCAGGGGAGATATTCTATCAAATCTGATGTCTTTAGCTTTGGTGTCTTGCTGCTAGAGATCATAAGTGGCAAGAGAAACACTGATTATGATCCCGAAAGTCCCGCCCCAAATTTGATTGGAAGAATTTGGGATTTGTGGAGAGAGGGGCAAGCACTAGGGATAGTCAATCCATCACTGCTTCAGTCATATCCTAGTGATGAAGTCTCAAGATGCATCCAGATTGCGCTACTCTGCATCCAAGAATGTGTAACAGATCGGCCTACCATGTCGGAAGTTGTTTTCATGTTGAGTAATGAAACATCTCTTCCTTTTCCTAAGAAACCTGCATTCATATTACAATCCAGCAATCCATCAACACCAGGACGAGGGCCTTATTCCGTAAATAATGTAACTATAATTATGCTCCAAGCACGATAA

Protein Analysis

841

Amino Acids

94.31

Weight (kDa)

6.3

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 79 - 184 5.6e-33 D-mannose binding lectin
S_locus_glycop PF00954 240 - 331 1.3e-19 S-locus glycoprotein domain
PAN_2 PF08276 355 - 420 2.3e-22 PAN-like domain
Pkinase PF00069 528 - 795 1.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 529 - 795 4.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 2437
Acc65I GGTACC 1 cut(s) 1347
AccB1I GGYRCC 1 cut(s) 1347
AccB7I CCANNNNNTGG 1 cut(s) 1534
AccIII TCCGGA 1 cut(s) 821
AciI CCGC 2 cut(s) 652, 2208
AclWI GGATC 6 cut(s) 257, 560, 603, 616, 832, 2189
AcsI RAATTY 7 cut(s) 285, 553, 930, 1494, 1896, 2215, 2229
AcuI CTGAAG 4 cut(s) 20, 160, 1718, 2270
AfaI GTAC 9 cut(s) 165, 218, 301, 539, 629, 715, 817, 1349, 1805
AfiI CCNNNNNNNGG 6 cut(s) 427, 575, 1065, 1362, 1534, 2471
AflIII ACRYGT 4 cut(s) 487, 534, 1421, 2085
AgsI TTSAA 9 cut(s) 112, 475, 558, 833, 1174, 1274, 1434, 1882, 1902
AjuI GAANNNNNNNTTGG 2 cut(s) 1596, 1628
AleI CACNNNNGTG 2 cut(s) 239, 1426
AloI GAACNNNNNNTCC 2 cut(s) 978, 1010
Alw26I GTCTC 5 cut(s) 149, 252, 376, 1927, 2314
AlwI GGATC 6 cut(s) 257, 560, 603, 616, 832, 2189
Ama87I CYCGRG 2 cut(s) 280, 1930
Aor13HI TCCGGA 1 cut(s) 821
AoxI GGCC 4 cut(s) 340, 919, 2363, 2474
ApeKI GCWGC 5 cut(s) 705, 856, 1319, 1767, 2155
ApoI RAATTY 7 cut(s) 285, 553, 930, 1494, 1896, 2215, 2229
ArsI GACNNNNNNTTYG 4 cut(s) 143, 175, 2118, 2150
AseI ATTAAT 1 cut(s) 1890
Asp718I GGTACC 1 cut(s) 1347
AspLEI GCGC 1 cut(s) 2332
AspS9I GGNCC 3 cut(s) 520, 920, 2474
AsuHPI GGTGA 8 cut(s) 143, 341, 366, 970, 1085, 1732, 1762, 2082
AsuNHI GCTAGC 2 cut(s) 838, 1964
AvaI CYCGRG 2 cut(s) 280, 1930
AvaII GGWCC 1 cut(s) 520
BamHI GGATCC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 1347
BanII GRGCYC 2 cut(s) 1054, 1635
BbsI GAAGAC 3 cut(s) 237, 1448, 2043
BbvI GCAGC 5 cut(s) 717, 868, 1331, 1754, 2142
BccI CCATC 7 cut(s) 122, 299, 1063, 1084, 1508, 2284, 2464
BceAI ACGGC 1 cut(s) 963
BciVI GTATCC 1 cut(s) 1249
BcoDI GTCTC 5 cut(s) 149, 252, 376, 1927, 2314
BfaI CTAG 7 cut(s) 839, 1637, 1683, 1965, 2159, 2261, 2298
BfuAI ACCTGC 1 cut(s) 2437
BfuI GTATCC 1 cut(s) 1249
BglII AGATCT 1 cut(s) 1451
BisI GCNGC 5 cut(s) 706, 857, 1320, 1768, 2156
BlsI GCNGC 5 cut(s) 707, 858, 1321, 1769, 2157
BmcAI AGTACT 1 cut(s) 301
Bme18I GGWCC 1 cut(s) 520
BmeT110I CYCGRG 2 cut(s) 280, 1930
BmgT120I GGNCC 3 cut(s) 520, 920, 2474
BmiI GGNNCC 5 cut(s) 610, 1053, 1349, 1634, 1745
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 7 cut(s) 129, 658, 1779, 1779, 2306, 2328, 2349
BmtI GCTAGC 2 cut(s) 842, 1968
BmuI ACTGGG 1 cut(s) 964
BpiI GAAGAC 3 cut(s) 237, 1448, 2043
BpmI CTGGAG 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 2296
BsaAI YACGTR 1 cut(s) 1301
BsaBI GATNNNNATC 3 cut(s) 309, 1793, 2193
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 337, 393, 523
BsaWI WCCGGW 1 cut(s) 821
Bsc4I CCNNNNNNNGG 6 cut(s) 427, 575, 1065, 1362, 1534, 2471
Bse1I ACTGG 4 cut(s) 76, 959, 1200, 1871
Bse3DI GCAATG 5 cut(s) 715, 1404, 1975, 1995, 2109
Bse8I GATNNNNATC 3 cut(s) 309, 1793, 2193
BseAI TCCGGA 1 cut(s) 821
BseDI CCNNGG 3 cut(s) 337, 393, 523
BseGI GGATG 7 cut(s) 188, 478, 818, 1055, 1095, 2319, 2340
BseJI GATNNNNATC 3 cut(s) 309, 1793, 2193
BseLI CCNNNNNNNGG 6 cut(s) 427, 575, 1065, 1362, 1534, 2471
BseMI GCAATG 5 cut(s) 715, 1404, 1975, 1995, 2109
BseMII CTCAG 6 cut(s) 240, 629, 1448, 1632, 1791, 2019
BseNI ACTGG 4 cut(s) 76, 959, 1200, 1871
BseXI GCAGC 5 cut(s) 717, 868, 1331, 1754, 2142
BsgI GTGCAG 1 cut(s) 1224
BshFI GGCC 4 cut(s) 342, 921, 2365, 2476
BshNI GGYRCC 1 cut(s) 1347
BsiHKCI CYCGRG 2 cut(s) 280, 1930
BsiSI CCGG 1 cut(s) 822
BslFI GGGAC 2 cut(s) 1967, 2190
BslI CCNNNNNNNGG 6 cut(s) 427, 575, 1065, 1362, 1534, 2471
BsmAI GTCTC 5 cut(s) 149, 252, 376, 1927, 2314
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 1967, 2190
BsmI GAATGC 2 cut(s) 31, 2432
BsnI GGCC 4 cut(s) 342, 921, 2365, 2476
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 2 cut(s) 280, 1930
Bsp1286I GDGCHC 2 cut(s) 1054, 1635
Bsp13I TCCGGA 1 cut(s) 821
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
Bsp19I CCATGG 2 cut(s) 393, 523
BspACI CCGC 2 cut(s) 652, 2208
BspANI GGCC 4 cut(s) 342, 921, 2365, 2476
BspCNI CTCAG 6 cut(s) 239, 628, 1449, 1631, 1790, 2018
BspEI TCCGGA 1 cut(s) 821
BspLI GGNNCC 5 cut(s) 610, 1053, 1349, 1634, 1745
BspMI ACCTGC 1 cut(s) 2437
BspOI GCTAGC 2 cut(s) 842, 1968
BspPI GGATC 6 cut(s) 257, 560, 603, 616, 832, 2189
BspT107I GGYRCC 1 cut(s) 1347
BspTNI GGTCTC 1 cut(s) 149
BsrDI GCAATG 5 cut(s) 715, 1404, 1975, 1995, 2109
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 4 cut(s) 76, 959, 1200, 1871
BssECI CCNNGG 3 cut(s) 337, 393, 523
BssMI GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
BssT1I CCWWGG 3 cut(s) 337, 393, 523
Bst4CI ACNGT 5 cut(s) 238, 452, 603, 1073, 1490
Bst6I CTCTTC 3 cut(s) 1014, 1776, 2415
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 1301
BstC8I GCNNGC 6 cut(s) 698, 840, 861, 970, 1966, 2256
BstDEI CTNAG 8 cut(s) 226, 615, 1260, 1457, 1618, 1777, 2005, 2421
BstDSI CCRYGG 2 cut(s) 393, 523
BstF5I GGATG 7 cut(s) 188, 478, 818, 1055, 1095, 2319, 2340
BstHHI GCGC 1 cut(s) 2332
BstKTI GATC 9 cut(s) 265, 568, 611, 827, 1454, 1797, 2166, 2197, 2362
BstMAI GTCTC 5 cut(s) 149, 252, 376, 1927, 2314
BstMBI GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
BstMWI GCNNNNNNNGC 7 cut(s) 702, 844, 853, 1316, 1328, 1468, 1477
BstNSI RCATGY 5 cut(s) 491, 538, 972, 1158, 2089
BstV1I GCAGC 5 cut(s) 717, 868, 1331, 1754, 2142
BstV2I GAAGAC 3 cut(s) 237, 1448, 2043
BstX2I RGATCY 2 cut(s) 608, 1451
BstXI CCANNNNNNTGG 2 cut(s) 1005, 1506
BstYI RGATCY 2 cut(s) 608, 1451
BsuI GTATCC 1 cut(s) 1249
BsuRI GGCC 4 cut(s) 342, 921, 2365, 2476
BtgI CCRYGG 2 cut(s) 393, 523
BtsCI GGATG 7 cut(s) 188, 478, 818, 1055, 1095, 2319, 2340
BtsI GCAGTG 1 cut(s) 2279
BtsIMutI CAGTG 4 cut(s) 1207, 1499, 2184, 2279
BveI ACCTGC 1 cut(s) 2437
Cac8I GCNNGC 6 cut(s) 698, 840, 861, 970, 1966, 2256
CfoI GCGC 1 cut(s) 2332
Cfr13I GGNCC 3 cut(s) 520, 920, 2474
CsiI ACCWGGT 3 cut(s) 420, 1235, 1746
Csp6I GTAC 9 cut(s) 164, 217, 300, 538, 628, 714, 816, 1348, 1804
CspCI CAANNNNNGTGG 2 cut(s) 2046, 2081
CviQI GTAC 9 cut(s) 164, 217, 300, 538, 628, 714, 816, 1348, 1804
DdeI CTNAG 8 cut(s) 226, 615, 1260, 1457, 1618, 1777, 2005, 2421
DpnI GATC 9 cut(s) 264, 567, 610, 826, 1453, 1796, 2165, 2196, 2361
DpnII GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
DraI TTTAAA 1 cut(s) 1705
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
Eam1104I CTCTTC 3 cut(s) 1014, 1776, 2415
EarI CTCTTC 3 cut(s) 1014, 1776, 2415
Eco130I CCWWGG 3 cut(s) 337, 393, 523
Eco147I AGGCCT 1 cut(s) 342
Eco24I GRGCYC 2 cut(s) 1054, 1635
Eco31I GGTCTC 1 cut(s) 149
Eco32I GATATC 2 cut(s) 804, 1210
Eco47I GGWCC 1 cut(s) 520
Eco57I CTGAAG 4 cut(s) 20, 160, 1718, 2270
Eco88I CYCGRG 2 cut(s) 280, 1930
EcoO109I RGGNCCY 1 cut(s) 2474
EcoRI GAATTC 2 cut(s) 285, 553
EcoRV GATATC 2 cut(s) 804, 1210
EcoT14I CCWWGG 3 cut(s) 337, 393, 523
EcoT22I ATGCAT 2 cut(s) 31, 2321
EcoT38I GRGCYC 2 cut(s) 1054, 1635
ErhI CCWWGG 3 cut(s) 337, 393, 523
Esp3I CGTCTC 1 cut(s) 252
FaqI GGGAC 2 cut(s) 1967, 2190
FauI CCCGC 1 cut(s) 2215
FauNDI CATATG 2 cut(s) 1195, 2077
Fnu4HI GCNGC 5 cut(s) 706, 857, 1320, 1768, 2156
FokI GGATG 7 cut(s) 175, 465, 805, 1042, 1102, 2306, 2327
FriOI GRGCYC 2 cut(s) 1054, 1635
Fsp4HI GCNGC 5 cut(s) 706, 857, 1320, 1768, 2156
FspBI CTAG 7 cut(s) 839, 1637, 1683, 1965, 2159, 2261, 2298
GlaI GCGC 1 cut(s) 2331
GluI GCNGC 5 cut(s) 706, 857, 1320, 1768, 2156
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 4 cut(s) 342, 921, 2365, 2476
HapII CCGG 1 cut(s) 822
HhaI GCGC 1 cut(s) 2332
Hin6I GCGC 1 cut(s) 2330
HinP1I GCGC 1 cut(s) 2330
HindIII AAGCTT 3 cut(s) 1595, 1819, 1852
HpaII CCGG 1 cut(s) 822
HphI GGTGA 8 cut(s) 143, 341, 366, 970, 1085, 1732, 1762, 2082
Hpy166II GTNNAC 5 cut(s) 20, 520, 662, 736, 787
Hpy8I GTNNAC 5 cut(s) 20, 520, 662, 736, 787
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 353, 1598, 1742
HpyCH4III ACNGT 5 cut(s) 238, 452, 603, 1073, 1490
HpyCH4IV ACGT 3 cut(s) 664, 1300, 1423
HpyF10VI GCNNNNNNNGC 7 cut(s) 702, 844, 853, 1316, 1328, 1468, 1477
HpyF3I CTNAG 8 cut(s) 226, 615, 1260, 1457, 1618, 1777, 2005, 2421
HpySE526I ACGT 3 cut(s) 664, 1300, 1423
HspAI GCGC 1 cut(s) 2330
Kpn2I TCCGGA 1 cut(s) 821
KpnI GGTACC 1 cut(s) 1351
Kzo9I GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
LmnI GCTCC 3 cut(s) 1057, 1638, 2514
Lsp1109I GCAGC 5 cut(s) 717, 868, 1331, 1754, 2142
LweI GCATC 7 cut(s) 129, 658, 1779, 1779, 2306, 2328, 2349
MabI ACCWGGT 3 cut(s) 420, 1235, 1746
MaeI CTAG 7 cut(s) 839, 1637, 1683, 1965, 2159, 2261, 2298
MaeII ACGT 3 cut(s) 664, 1300, 1423
MaeIII GTNAC 7 cut(s) 131, 380, 899, 1589, 2088, 2353, 2494
MalI GATC 9 cut(s) 264, 567, 610, 826, 1453, 1796, 2165, 2196, 2361
MboI GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 2 cut(s) 608, 1451
MhlI GDGCHC 2 cut(s) 1054, 1635
MlyI GAGTC 6 cut(s) 219, 1031, 1153, 1769, 1922, 2010
MmeI TCCRAC 4 cut(s) 171, 187, 2050, 2355
Mph1103I ATGCAT 2 cut(s) 31, 2321
MroI TCCGGA 1 cut(s) 821
MseI TTAA 8 cut(s) 320, 432, 590, 656, 1013, 1250, 1704, 1890
MslI CAYNNNNRTG 4 cut(s) 239, 453, 1060, 1426
MspI CCGG 1 cut(s) 822
MunI CAATTG 1 cut(s) 85
Mva1269I GAATGC 2 cut(s) 31, 2432
MwoI GCNNNNNNNGC 7 cut(s) 702, 844, 853, 1316, 1328, 1468, 1477
NcoI CCATGG 2 cut(s) 393, 523
NdeI CATATG 2 cut(s) 1195, 2077
NdeII GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
NheI GCTAGC 2 cut(s) 838, 1964
NlaIV GGNNCC 5 cut(s) 610, 1053, 1349, 1634, 1745
NmuCI GTSAC 3 cut(s) 131, 899, 2088
NsiI ATGCAT 2 cut(s) 31, 2321
NspI RCATGY 5 cut(s) 491, 538, 972, 1158, 2089
OliI CACNNNNGTG 2 cut(s) 239, 1426
PaeI GCATGC 1 cut(s) 972
PaeR7I CTCGAG 1 cut(s) 1930
PceI AGGCCT 1 cut(s) 342
PciI ACATGT 3 cut(s) 487, 534, 2085
PctI GAATGC 2 cut(s) 31, 2432
PfeI GAWTC 4 cut(s) 171, 410, 1679, 1982
PflMI CCANNNNNTGG 1 cut(s) 1534
PfoI TCCNGGA 3 cut(s) 405, 568, 976
PkrI GCNGC 5 cut(s) 707, 858, 1321, 1769, 2157
PleI GAGTC 6 cut(s) 218, 1030, 1152, 1769, 1922, 2010
PpsI GAGTC 6 cut(s) 218, 1030, 1152, 1769, 1922, 2010
Ppu21I YACGTR 1 cut(s) 1301
PscI ACATGT 3 cut(s) 487, 534, 2085
PshBI ATTAAT 1 cut(s) 1890
PspN4I GGNNCC 5 cut(s) 610, 1053, 1349, 1634, 1745
PspPI GGNCC 3 cut(s) 520, 920, 2474
PsuI RGATCY 2 cut(s) 608, 1451
RsaI GTAC 9 cut(s) 165, 218, 301, 539, 629, 715, 817, 1349, 1805
RsaNI GTAC 9 cut(s) 164, 217, 300, 538, 628, 714, 816, 1348, 1804
RseI CAYNNNNRTG 4 cut(s) 239, 453, 1060, 1426
SaqAI TTAA 8 cut(s) 320, 432, 590, 656, 1013, 1250, 1704, 1890
SatI GCNGC 5 cut(s) 706, 857, 1320, 1768, 2156
Sau3AI GATC 9 cut(s) 262, 565, 608, 824, 1451, 1794, 2163, 2194, 2359
Sau96I GGNCC 3 cut(s) 520, 920, 2474
ScaI AGTACT 1 cut(s) 301
SchI GAGTC 6 cut(s) 219, 1031, 1153, 1769, 1922, 2010
SduI GDGCHC 2 cut(s) 1054, 1635
SexAI ACCWGGT 3 cut(s) 420, 1235, 1746
SfaNI GCATC 7 cut(s) 129, 658, 1779, 1779, 2306, 2328, 2349
Sfr274I CTCGAG 1 cut(s) 1930
SinI GGWCC 1 cut(s) 520
SlaI CTCGAG 1 cut(s) 1930
SmiMI CAYNNNNRTG 4 cut(s) 239, 453, 1060, 1426
SmlI CTYRAG 2 cut(s) 1930, 2311
SmoI CTYRAG 2 cut(s) 1930, 2311
SphI GCATGC 1 cut(s) 972
SseBI AGGCCT 1 cut(s) 342
SsiI CCGC 2 cut(s) 652, 2208
SspI AATATT 3 cut(s) 624, 1105, 1885
SspMI CTAG 7 cut(s) 839, 1637, 1683, 1965, 2159, 2261, 2298
StuI AGGCCT 1 cut(s) 342
StyI CCWWGG 3 cut(s) 337, 393, 523
TaaI ACNGT 5 cut(s) 238, 452, 603, 1073, 1490
TaiI ACGT 3 cut(s) 667, 1303, 1426
TaqI TCGA 3 cut(s) 1125, 1773, 1931
TatI WGTACW 3 cut(s) 163, 299, 627
TfiI GAWTC 4 cut(s) 171, 410, 1679, 1982
Tru1I TTAA 8 cut(s) 320, 432, 590, 656, 1013, 1250, 1704, 1890
Tru9I TTAA 8 cut(s) 320, 432, 590, 656, 1013, 1250, 1704, 1890
TscAI CASTG 4 cut(s) 1207, 1506, 2191, 2286
TseFI GTSAC 3 cut(s) 131, 899, 2088
TseI GCWGC 5 cut(s) 705, 856, 1319, 1767, 2155
Tsp45I GTSAC 3 cut(s) 131, 899, 2088
TspGWI ACGGA 1 cut(s) 2473
TspRI CASTG 4 cut(s) 1207, 1506, 2191, 2286
Van91I CCANNNNNTGG 1 cut(s) 1534
VpaK11BI GGWCC 1 cut(s) 520
VspI ATTAAT 1 cut(s) 1890
XapI RAATTY 7 cut(s) 285, 553, 930, 1494, 1896, 2215, 2229
XceI RCATGY 5 cut(s) 491, 538, 972, 1158, 2089
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XhoI CTCGAG 1 cut(s) 1930
XspI CTAG 7 cut(s) 839, 1637, 1683, 1965, 2159, 2261, 2298
ZrmI AGTACT 1 cut(s) 301
Zsp2I ATGCAT 2 cut(s) 31, 2321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.