MD00G1203200.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000099
Physical Location & Seq
Forward (+)
48693060 .. 48696041
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1203200.v1.1.491

Sequence Viewer

Length: 2160 bp
ATGAATTTTCTGAAAGGTCTGCTAAATGTTTTAGTTCTTCAGCACCTCCTTCTGCAGCTCTGCTCTTCCTTGGACACCATAAAATTTGATCAACCCGTCGGAGACGGTGATTTCTTGGTGTCCAAGAACGAGACCTTTGTCCTGGGATTCTTTAGTCCCGGGATATCTACCAACCGCTATGTCGGAATTTGGTACAAATTTTCGGAAGACTTGGTTGTGTGGGTGGCCAACAGAGATAAGCCCATCAATGGTAGGTCAGGAGTTCTCTCAATAGGTTCTGATGGAAACCTAGTGCTGCAAGCCAATACTAGTCAAGGCTTTATTCCTCTATGGTCAACAAATGTTTCGATATCATCAGACAGCAACGATAATATTTTTGCGCAAATTCTTGATTCAGGAAACCTTGTTTTGGCTGGCAGCCAGGATGTGTTTTGGCAGAGCACTGATCACCCTACAAATATTCTTCTTCCGAATATGAAAGTCGGGTTGGACAGAAGAAGTGGCATAAACCGGTTCCTCACGTGTTGGAACTCGGACAATGATCCTGGGACGGGGAATGTTTCGTTGAGAATGGACTCGAATGGATCGCCTCAGTTGATCTTGTACAAGGATGACGCTAAGTGGTGGAGGTCAGGACAGTGGAACGGGATTCAATGGGGCGGCATACCTGCCATGCATCGGAGCGTTTGGACTGTTCTCGACCTTTTAATTTACTCGGTGATCACAATAGACGGGTCGGGAACACTCAACCAGCTATCATGGCAGGACCAGCAGCACCAGTGGGTTTCGCTTTGGTCGGCACCATTGGATGCTTGTGACAGCTATGGCAAGTGTGGTCAATTTGGCAATTGCAATCCTTACACCAACAGTGGGTTCAACTGCACATGCTACCCCGGATATGAACCAACCTCACCGCAGGATTGGGATTTAAGAGATGGGACAGATGGGTGCAAGAGGCCGCAGGGCTCGCCGTCCATGTGCAGGAACGGCGAGGGCTTTGTGAAGATGGAAAATGTAAAGGTTCCAGACACGTCCTCTATAAAATTGGAGAATAATTTGAGCTTAGAAGCGTGCGAGGAGGAGTGCTTGAAGAACTGCTCGTGCTTGGCATATGCGAGTGCAAATGTGAGGAATGGAGGGACTGGATGCATGACATGGTACAGAGATTTGGTGGATACTAAGCAGTTCACAGAAGGCCGGCAAGATTTGTACATTCGCGTTGATGCAGTAGTTTTAGTTCTATGCTGGTTCGGGAAAAGGAGCAGGAAAGGGAGAGGAGGAGAACCCAGATTTCTGAACGATCCCGCTGCTTCTGGTGTTCGAAGCTATGAAGATTTGCCAATAAAAAATGAGGTTGATGAACACAGAGGAGACACAGATTTACCTTTTTTCGATTTAACCACTGTGGTTGCAGCCACAGAAAACTTCTCTTCTGCTAACAAGCTTGGACACGGCGGCTTCGGCACGGTATATAAGGGATGTCTAGCTGATGGACAGGACATAGCTGTCAAAAGATTATCGAGAAATTCAGGACAAGGCGTAGATGAATTTAAGAACGAAGTAATGCTTATAGCAAAGCTTCAGCATAGAAATCTTGTGAGGCTTTTGGGTTGGTGCATACATAAAGAAGAGAGGATGCTAATCTATGAATACATGCCGAATCGCAGCTTGGACTTATGCATTTTCGATAAAAACAGAAAGTCGTTGTTAGATTGGAGAAAAAGATTTCAAATTATCATTGGGATTGCTCGAGGCGTCCTATATCTTCATCAAGATTCGAGACTAAAAATAATCCACAGGGATTTGAAAGCAAGCAATGTTTTACTGGATGGTTCAATGAACCCAAAAATATCTGATTTTGGCATGGCAAGAATGTTCGGGGATGACCAAATTGAAGCAAACACGAATAGAATTGTTGGCACCTATGGTTACATGTCATCGGAGTATGCTATGGATGGGCTATATTCCACAAAATCCGATGTGTTTAGCTTCGGAGTCTTGGCATTAGAGATCATTAGCGGCAGAAAGAACAGCTTCCAATTCGAAAACACCTCTCTGAATTTGGTTGGACTTATATGGGACTTGTGGACAGAAGGAAAAGTCTTGGACATAGTTGGTTCATCACTGAACCAGTCGTATTCGACTCATGAAGTTATTTAG

Protein Analysis

720

Amino Acids

80.42

Weight (kDa)

5.6

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 176 1e-29 D-mannose binding lectin
S_locus_glycop PF00954 209 - 303 2.1e-15 S-locus glycoprotein domain
PAN_2 PF08276 327 - 393 6.7e-21 PAN-like domain
Pkinase PF00069 477 - 677 7e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 479 - 680 8.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11340 AT1G11340 AT1G11340 AT1G11410 AT1G11410 AT1G11410 AT1G11410
fragaria_vesca FvH4_3g03230 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03231 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03240 FvH4_3g03241 FvH4_3g03242 FvH4_3g03243 FvH4_3g03300 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03301 FvH4_3g03310 FvH4_3g03310 FvH4_6g07960
malus_domestica MD00G1203200.v1.1 MD02G1260900.v1.1 MD05G1263100.v1.1 MD05G1332300.v1.1 MD05G1332400.v1.1 MD05G1332600.v1.1 MD05G1332700.v1.1 MD05G1333400.v1.1 MD05G1333500.v1.1 MD05G1333700.v1.1 MD05G1334000.v1.1 MD10G1291100.v1.1 MD10G1291200.v1.1 MD10G1307900.v1.1 MD10G1308000.v1.1 MD10G1308200.v1.1 MD10G1308700.v1.1 MD17G1273200.v1.1
prunus_persica Prupe.4G031200_v2.0.a1 Prupe.4G031400_v2.0.a1 Prupe.4G031500_v2.0.a1 Prupe.4G031600_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.4G031800_v2.0.a1 Prupe.8G238600_v2.0.a1
pyrus_communis pycom02g22290 pycom02g22300 pycom05g30390 pycom05g30430 pycom05g30450 pycom05g30470 pycom05g30510 pycom05g30530 pycom05g30560 pycom05g30570 pycom10g24330 pycom10g25940 pycom10g25950 pycom10g25960 pycom10g25970 pycom10g25980 pycom10g25990 pycom10g26000 pycom10g26010 pycom10g26020 pycom10g26030 pycom17g27160
rosa_chinensis RchiOBHm_Chr2g0119951 RchiOBHm_Chr2g0119961 RchiOBHm_Chr2g0119981 RchiOBHm_Chr2g0120011 RchiOBHm_Chr2g0120061 RchiOBHm_Chr2g0120071 RchiOBHm_Chr3g0477341 RchiOBHm_Chr4g0411711 RchiOBHm_Chr4g0411831 RchiOBHm_Chr4g0411951 RchiOBHm_Chr5g0004871 RchiOBHm_Chr5g0004881 RchiOBHm_Chr5g0004891 RchiOBHm_Chr5g0004921 RchiOBHm_Chr5g0004931 RchiOBHm_Chr5g0004941 RchiOBHm_Chr5g0004961 RchiOBHm_Chr5g0004971 RchiOBHm_Chr5g0004991 RchiOBHm_Chr5g0005011 RchiOBHm_Chr5g0005031 RchiOBHm_Chr5g0005041 RchiOBHm_Chr5g0005051 RchiOBHm_Chr5g0034351 RchiOBHm_Chr5g0034371 RchiOBHm_Chr5g0035421 RchiOBHm_Chr5g0035471 RchiOBHm_Chr5g0035541 RchiOBHm_Chr5g0035551 RchiOBHm_Chr5g0035581 RchiOBHm_Chr5g0035651 RchiOBHm_Chr5g0035661 RchiOBHm_Chr5g0035791 RchiOBHm_Chr5g0035821 RchiOBHm_Chr5g0035881 RchiOBHm_Chr5g0035941 RchiOBHm_Chr5g0066181
rosa_laevigata RLG00000008339 RLG00000018505 RLG00000018510 RLG00000018738 RLG00000018741 RLG00000023265 RLG00000031268 RLG00000031270 RLG00000031272 RLG00000031274 RLG00000031275 RLG00000031278 RLG00000031280 RLG00000031281 RLG00000031282 RLG00000032920 RLG00000033622 RLG00000033628 RLG00000033641
rosa_multiflora Rmu_co8015890.1_g000001 Rmu_co8119476.1_g000001 Rmu_co8172744.1_g000001 Rmu_co8181302.1_g000001 Rmu_co8369629.1_g000001 Rmu_co8379407.1_g000001 Rmu_co8412887.1_g000001 Rmu_sc0000084.1_g000011 Rmu_sc0000084.1_g000012 Rmu_sc0000084.1_g000031 Rmu_sc0000593.1_g000007 Rmu_sc0000593.1_g000011 Rmu_sc0000593.1_g000014 Rmu_sc0000657.1_g000020 Rmu_sc0000711.1_g000010 Rmu_sc0000711.1_g000048 Rmu_sc0000813.1_g000017 Rmu_sc0001493.1_g000041 Rmu_sc0002935.1_g000014 Rmu_sc0002935.1_g000019 Rmu_sc0003096.1_g000002 Rmu_sc0003096.1_g000009 Rmu_sc0003096.1_g000010 Rmu_sc0003096.1_g000014 Rmu_sc0003541.1_g000052 Rmu_sc0004315.1_g000002 Rmu_sc0006173.1_g000007 Rmu_sc0006173.1_g000035 Rmu_sc0006369.1_g000003 Rmu_sc0007790.1_g000002 Rmu_sc0009702.1_g000001 Rmu_sc0009702.1_g000002 Rmu_sc0009702.1_g000004 Rmu_sc0009702.1_g000006 Rmu_sc0010714.1_g000001 Rmu_sc0010714.1_g000002 Rmu_sc0010714.1_g000007 Rmu_sc0010714.1_g000008 Rmu_sc0010714.1_g000009 Rmu_sc0011169.1_g000008 Rmu_sc0013877.1_g000001 Rmu_sc0013932.1_g000001 Rmu_sc0015231.1_g000005 Rmu_sc0016543.1_g000002 Rmu_sc0016543.1_g000003 Rmu_sc0017275.1_g000001 Rmu_sc0026861.1_g000001 Rmu_sc0030606.1_g000001
rosa_roxburghii Rroxscaffold_1G00044840 Rroxscaffold_1G00044850 Rroxscaffold_1G00044900 Rroxscaffold_1G00044980 Rroxscaffold_1G00044990 Rroxscaffold_1G00070600 Rroxscaffold_1G00070610 Rroxscaffold_1G00070630 Rroxscaffold_1G00070650 Rroxscaffold_1G00070680 Rroxscaffold_1G00070700 Rroxscaffold_1G00070710 Rroxscaffold_1G00070740 Rroxscaffold_1G00070750 Rroxscaffold_2G00123770 Rroxscaffold_2G00123820 Rroxscaffold_2G00123830 Rroxscaffold_2G00123900 Rroxscaffold_2G00123920 Rroxscaffold_4G00293110 Rroxscaffold_5G00353540 Rroxscaffold_5G00353620
rosa_rugosa Rorug01G0307800 Rorug02G0225800 Rorug02G0225900 Rorug04G0414800 Rorug04G0414800 Rorug04G0414800 Rorug04G0414900 Rorug04G0415000 Rorug05G0151600
rosa_samantha Rh3DG237000 Rh4BG166800 Rh5BG042300 Rh5CG045900 Rh5CG046000 Rh5CG046100 Rh5CG046200 Rh5CG046300 Rh5CG046400 Rh5CG046500 Rh5CG046600 Rh5CG046800 Rh5CG047000 Rh5CG047100 Rh5CG047300 Rh5CG047500 Rh5CG047600 Rh5CG267500 Rh5CG276200
rosa_wichuraiana Rw0G000290 Rw0G007740 Rw0G022180 Rw1G002050 Rw2G022580 Rw2G022600 Rw2G022620 Rw2G022650 Rw2G022660 Rw4G014100 Rw4G014220 Rw5G004100 Rw5G004110 Rw5G004120 Rw5G004130 Rw5G004140 Rw5G004160 Rw5G004170 Rw5G016790 Rw5G021680 Rw5G022380 Rw5G022450 Rw5G022470 Rw5G022490 Rw5G022540 Rw5G022570 Rw5G050160 Rw7G005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1505
Acc16I TGCGCA 1 cut(s) 381
Acc36I ACCTGC 1 cut(s) 676
AccB1I GGYRCC 2 cut(s) 799, 1919
AccII CGCG 1 cut(s) 1218
AciI CCGC 7 cut(s) 175, 660, 914, 959, 1305, 1455, 2019
AclWI GGATC 3 cut(s) 536, 592, 1295
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 8 cut(s) 4, 83, 186, 197, 384, 1525, 1547, 2059
AcuI CTGAAG 2 cut(s) 23, 1565
AcvI CACGTG 1 cut(s) 522
AcyI GRCGYC 1 cut(s) 1755
AfaI GTAC 4 cut(s) 194, 605, 1160, 1211
AfiI CCNNNNNNNGG 6 cut(s) 248, 409, 551, 678, 870, 981
AflIII ACRYGT 3 cut(s) 521, 1029, 1932
AgeI ACCGGT 1 cut(s) 510
AgsI TTSAA 7 cut(s) 653, 877, 1090, 1730, 1807, 1836, 1895
AhlI ACTAGT 1 cut(s) 308
AjiI CACGTC 1 cut(s) 1032
AjnI CCWGG 3 cut(s) 141, 420, 544
AjuI GAANNNNNNNTTGG 6 cut(s) 470, 502, 857, 889, 1652, 1684
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 4 cut(s) 96, 125, 1365, 1774
AlwI GGATC 3 cut(s) 536, 592, 1295
Ama87I CYCGRG 2 cut(s) 158, 1749
AoxI GGCC 3 cut(s) 225, 956, 1195
ApeKI GCWGC 7 cut(s) 55, 295, 417, 772, 1307, 1412, 1665
ApoI RAATTY 8 cut(s) 4, 83, 186, 197, 384, 1525, 1547, 2059
AsiGI ACCGGT 1 cut(s) 510
Asp700I GAANNNNTTC 1 cut(s) 2033
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 766
AsuC2I CCSGG 3 cut(s) 159, 160, 894
AsuHPI GGTGA 4 cut(s) 119, 440, 730, 903
AsuII TTCGAA 2 cut(s) 1321, 2043
AvaI CYCGRG 2 cut(s) 158, 1749
AvaII GGWCC 1 cut(s) 766
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 2 cut(s) 799, 1919
BanII GRGCYC 1 cut(s) 968
BauI CACGAG 1 cut(s) 1099
BbrPI CACGTG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 443
BbvI GCAGC 7 cut(s) 67, 282, 429, 784, 1294, 1424, 1677
BccI CCATC 8 cut(s) 251, 275, 929, 938, 1000, 1484, 1823, 1949
BceAI ACGGC 3 cut(s) 955, 1003, 1468
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BciT130I CCWGG 3 cut(s) 143, 422, 546
BciVI GTATCC 1 cut(s) 1168
BclI TGATCA 3 cut(s) 88, 445, 720
BcnI CCSGG 3 cut(s) 159, 160, 894
BcoDI GTCTC 4 cut(s) 96, 125, 1365, 1774
BcuI ACTAGT 1 cut(s) 308
BfaI CTAG 3 cut(s) 290, 309, 1484
BfmI CTRYAG 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 1168
Bme1390I CCNGG 6 cut(s) 143, 159, 160, 422, 546, 894
Bme18I GGWCC 1 cut(s) 766
BmeT110I CYCGRG 2 cut(s) 158, 1749
BmgBI CACGTC 1 cut(s) 1032
BmgT120I GGNCC 1 cut(s) 766
BmiI GGNNCC 4 cut(s) 515, 801, 1023, 1921
BmrFI CCNGG 6 cut(s) 143, 159, 160, 422, 546, 894
BmsI GCATC 5 cut(s) 685, 799, 1136, 1213, 1626
BoxI GACNNNNGTC 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 213
Bpu14I TTCGAA 2 cut(s) 1321, 2043
BpuMI CCSGG 3 cut(s) 159, 160, 894
BsaAI YACGTR 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 1640
BsaHI GRCGYC 1 cut(s) 1755
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 5 cut(s) 69, 142, 158, 545, 892
BsaWI WCCGGW 1 cut(s) 510
Bsc4I CCNNNNNNNGG 6 cut(s) 248, 409, 551, 678, 870, 981
Bse118I RCCGGY 2 cut(s) 510, 1197
Bse1I ACTGG 4 cut(s) 778, 1147, 1830, 2131
Bse3DI GCAATG 1 cut(s) 1822
Bse8I GATNNNNATC 1 cut(s) 1640
BseBI CCWGG 3 cut(s) 143, 422, 546
BseDI CCNNGG 5 cut(s) 69, 142, 158, 545, 892
BseGI GGATG 9 cut(s) 430, 616, 814, 1151, 1484, 1641, 1834, 1888, 1960
BseJI GATNNNNATC 1 cut(s) 1640
BseLI CCNNNNNNNGG 6 cut(s) 248, 409, 551, 678, 870, 981
BseMI GCAATG 1 cut(s) 1822
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 4 cut(s) 778, 1147, 1830, 2131
BseRI GAGGAG 5 cut(s) 1091, 1094, 1290, 1293, 1383
BseXI GCAGC 7 cut(s) 67, 282, 429, 784, 1294, 1424, 1677
BsgI GTGCAG 2 cut(s) 865, 1000
Bsh1236I CGCG 1 cut(s) 1218
BshFI GGCC 3 cut(s) 227, 958, 1197
BshNI GGYRCC 2 cut(s) 799, 1919
BshTI ACCGGT 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 2 cut(s) 158, 1749
BsiSI CCGG 4 cut(s) 159, 511, 894, 1198
BslFI GGGAC 5 cut(s) 141, 562, 952, 1153, 2093
BslI CCNNNNNNNGG 6 cut(s) 248, 409, 551, 678, 870, 981
BsmAI GTCTC 4 cut(s) 96, 125, 1365, 1774
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 5 cut(s) 141, 562, 952, 1153, 2093
BsnI GGCC 3 cut(s) 227, 958, 1197
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 2 cut(s) 158, 1749
Bsp119I TTCGAA 2 cut(s) 1321, 2043
Bsp1286I GDGCHC 2 cut(s) 443, 968
Bsp1407I TGTACA 2 cut(s) 603, 1209
Bsp143I GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
BspACI CCGC 7 cut(s) 175, 660, 914, 959, 1305, 1455, 2019
BspANI GGCC 3 cut(s) 227, 958, 1197
BspCNI CTCAG 1 cut(s) 604
BspFNI CGCG 1 cut(s) 1218
BspHI TCATGA 1 cut(s) 2146
BspLI GGNNCC 4 cut(s) 515, 801, 1023, 1921
BspMAI CTGCAG 1 cut(s) 57
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 536, 592, 1295
BspQI GCTCTTC 1 cut(s) 70
BspT104I TTCGAA 2 cut(s) 1321, 2043
BspT107I GGYRCC 2 cut(s) 799, 1919
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 1822
BsrFI RCCGGY 2 cut(s) 510, 1197
BsrGI TGTACA 2 cut(s) 603, 1209
BsrI ACTGG 4 cut(s) 778, 1147, 1830, 2131
BssAI RCCGGY 2 cut(s) 510, 1197
BssECI CCNNGG 5 cut(s) 69, 142, 158, 545, 892
BssMI GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
BssNI GRCGYC 1 cut(s) 1755
BssSI CACGAG 1 cut(s) 1099
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 1099
Bst2UI CCWGG 3 cut(s) 143, 422, 546
Bst4CI ACNGT 6 cut(s) 107, 639, 694, 869, 1405, 1468
Bst6I CTCTTC 3 cut(s) 70, 1435, 1623
BstACI GRCGYC 1 cut(s) 1755
BstAUI TGTACA 2 cut(s) 603, 1209
BstBAI YACGTR 1 cut(s) 522
BstBI TTCGAA 2 cut(s) 1321, 2043
BstC8I GCNNGC 6 cut(s) 300, 415, 968, 1072, 1199, 1813
BstDEI CTNAG 4 cut(s) 591, 618, 1063, 1179
BstF5I GGATG 9 cut(s) 430, 616, 814, 1151, 1484, 1641, 1834, 1888, 1960
BstFNI CGCG 1 cut(s) 1218
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 8 cut(s) 91, 448, 544, 587, 600, 723, 1303, 2013
BstMAI GTCTC 4 cut(s) 96, 125, 1365, 1774
BstMBI GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
BstMWI GCNNNNNNNGC 5 cut(s) 760, 769, 967, 987, 1461
BstNI CCWGG 3 cut(s) 143, 422, 546
BstNSI RCATGY 3 cut(s) 888, 1657, 1936
BstPAI GACNNNNGTC 1 cut(s) 137
BstSCI CCNGG 6 cut(s) 141, 157, 158, 420, 544, 892
BstSFI CTRYAG 1 cut(s) 53
BstUI CGCG 1 cut(s) 1218
BstV1I GCAGC 7 cut(s) 67, 282, 429, 784, 1294, 1424, 1677
BstV2I GAAGAC 1 cut(s) 213
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 3 cut(s) 227, 958, 1197
BtrI CACGTC 1 cut(s) 1032
BtsCI GGATG 9 cut(s) 430, 616, 814, 1151, 1484, 1641, 1834, 1888, 1960
BtsIMutI CAGTG 6 cut(s) 441, 644, 785, 874, 1401, 2123
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 6 cut(s) 300, 415, 968, 1072, 1199, 1813
CciI TCATGA 1 cut(s) 2146
CfoI GCGC 1 cut(s) 382
Cfr10I RCCGGY 2 cut(s) 510, 1197
Cfr13I GGNCC 1 cut(s) 766
Cfr9I CCCGGG 1 cut(s) 158
CseI GACGC 2 cut(s) 623, 1744
Csp6I GTAC 4 cut(s) 193, 604, 1159, 1210
CspAI ACCGGT 1 cut(s) 510
CspCI CAANNNNNGTGG 2 cut(s) 1390, 1425
CviQI GTAC 4 cut(s) 193, 604, 1159, 1210
DdeI CTNAG 4 cut(s) 591, 618, 1063, 1179
DpnI GATC 8 cut(s) 90, 447, 543, 586, 599, 722, 1302, 2012
DpnII GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
DrdI GACNNNNNNGTC 1 cut(s) 1505
DseDI GACNNNNNNGTC 1 cut(s) 1505
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 3 cut(s) 70, 1435, 1623
EarI CTCTTC 3 cut(s) 70, 1435, 1623
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 1 cut(s) 968
Eco31I GGTCTC 1 cut(s) 125
Eco32I GATATC 2 cut(s) 165, 351
Eco47I GGWCC 1 cut(s) 766
Eco57I CTGAAG 2 cut(s) 23, 1565
Eco72I CACGTG 1 cut(s) 522
Eco88I CYCGRG 2 cut(s) 158, 1749
EcoRII CCWGG 3 cut(s) 141, 420, 544
EcoRV GATATC 2 cut(s) 165, 351
EcoT14I CCWWGG 1 cut(s) 69
EcoT22I ATGCAT 3 cut(s) 678, 1151, 1682
EcoT38I GRGCYC 1 cut(s) 968
ErhI CCWWGG 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 96
FalI AAGNNNNNCTT 4 cut(s) 1551, 1583, 2018, 2050
FaqI GGGAC 5 cut(s) 141, 562, 952, 1153, 2093
FauI CCCGC 1 cut(s) 1312
FauNDI CATATG 1 cut(s) 1111
FbaI TGATCA 3 cut(s) 88, 445, 720
FokI GGATG 9 cut(s) 437, 623, 821, 1158, 1491, 1648, 1841, 1895, 1967
FriOI GRGCYC 1 cut(s) 968
FspBI CTAG 3 cut(s) 290, 309, 1484
FspI TGCGCA 1 cut(s) 381
GlaI GCGC 1 cut(s) 381
HaeIII GGCC 3 cut(s) 227, 958, 1197
HapII CCGG 4 cut(s) 159, 511, 894, 1198
HgaI GACGC 2 cut(s) 623, 1744
HhaI GCGC 1 cut(s) 382
Hin1I GRCGYC 1 cut(s) 1755
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 1442, 1577
HinfI GANTC 8 cut(s) 147, 392, 575, 649, 1660, 1775, 1995, 2143
HpaII CCGG 4 cut(s) 159, 511, 894, 1198
HphI GGTGA 4 cut(s) 119, 440, 730, 903
Hpy166II GTNNAC 3 cut(s) 336, 1188, 2088
Hpy8I GTNNAC 3 cut(s) 336, 1188, 2088
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 3 cut(s) 59, 1187, 2087
HpyCH4III ACNGT 6 cut(s) 107, 639, 694, 869, 1405, 1468
HpyCH4IV ACGT 2 cut(s) 521, 1031
HpyF10VI GCNNNNNNNGC 5 cut(s) 760, 769, 967, 987, 1461
HpyF3I CTNAG 4 cut(s) 591, 618, 1063, 1179
HpySE526I ACGT 2 cut(s) 521, 1031
Hsp92I GRCGYC 1 cut(s) 1755
HspAI GCGC 1 cut(s) 380
KroI GCCGGC 1 cut(s) 1197
KroNI GCCGGC 1 cut(s) 1199
Ksp22I TGATCA 3 cut(s) 88, 445, 720
Kzo9I GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 681, 1260
Lsp1109I GCAGC 7 cut(s) 67, 282, 429, 784, 1294, 1424, 1677
LweI GCATC 5 cut(s) 685, 799, 1136, 1213, 1626
MaeI CTAG 3 cut(s) 290, 309, 1484
MaeII ACGT 2 cut(s) 521, 1031
MaeIII GTNAC 2 cut(s) 815, 1928
MalI GATC 8 cut(s) 90, 447, 543, 586, 599, 722, 1302, 2012
MboI GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
MfeI CAATTG 1 cut(s) 847
MhlI GDGCHC 2 cut(s) 443, 968
MlsI TGGCCA 1 cut(s) 227
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 3 cut(s) 569, 2004, 2137
MmeI TCCRAC 5 cut(s) 79, 163, 468, 506, 2047
Mox20I TGGCCA 1 cut(s) 227
Mph1103I ATGCAT 3 cut(s) 678, 1151, 1682
MroNI GCCGGC 1 cut(s) 1197
MroXI GAANNNNTTC 1 cut(s) 2033
MscI TGGCCA 1 cut(s) 227
MseI TTAA 4 cut(s) 707, 929, 1397, 1551
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 1307
MspI CCGG 4 cut(s) 159, 511, 894, 1198
MspR9I CCNGG 6 cut(s) 143, 159, 160, 422, 546, 894
MunI CAATTG 1 cut(s) 847
MvaI CCWGG 3 cut(s) 143, 422, 546
MvnI CGCG 1 cut(s) 1218
MwoI GCNNNNNNNGC 5 cut(s) 760, 769, 967, 987, 1461
NaeI GCCGGC 1 cut(s) 1199
NciI CCSGG 3 cut(s) 159, 160, 894
NdeI CATATG 1 cut(s) 1111
NdeII GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
NgoMIV GCCGGC 1 cut(s) 1197
NlaIV GGNNCC 4 cut(s) 515, 801, 1023, 1921
NmuCI GTSAC 1 cut(s) 815
NsbI TGCGCA 1 cut(s) 381
NsiI ATGCAT 3 cut(s) 678, 1151, 1682
NspI RCATGY 3 cut(s) 888, 1657, 1936
NspV TTCGAA 2 cut(s) 1321, 2043
PaeR7I CTCGAG 1 cut(s) 1749
PagI TCATGA 1 cut(s) 2146
PciI ACATGT 1 cut(s) 1932
PciSI GCTCTTC 1 cut(s) 70
PdiI GCCGGC 1 cut(s) 1199
PdmI GAANNNNTTC 1 cut(s) 2033
PfeI GAWTC 5 cut(s) 147, 392, 649, 1660, 1775
PinAI ACCGGT 1 cut(s) 510
PleI GAGTC 3 cut(s) 569, 2003, 2137
PmaCI CACGTG 1 cut(s) 522
PmlI CACGTG 1 cut(s) 522
PpsI GAGTC 3 cut(s) 569, 2003, 2137
Ppu21I YACGTR 1 cut(s) 522
PscI ACATGT 1 cut(s) 1932
PshAI GACNNNNGTC 1 cut(s) 137
Psp6I CCWGG 3 cut(s) 141, 420, 544
PspCI CACGTG 1 cut(s) 522
PspGI CCWGG 3 cut(s) 141, 420, 544
PspN4I GGNNCC 4 cut(s) 515, 801, 1023, 1921
PspPI GGNCC 1 cut(s) 766
PspXI VCTCGAGB 1 cut(s) 1749
PsrI GAACNNNNNNTAC 2 cut(s) 1221, 1253
PstI CTGCAG 1 cut(s) 57
RsaI GTAC 4 cut(s) 194, 605, 1160, 1211
RsaNI GTAC 4 cut(s) 193, 604, 1159, 1210
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 4 cut(s) 707, 929, 1397, 1551
Sau3AI GATC 8 cut(s) 88, 445, 541, 584, 597, 720, 1300, 2010
Sau96I GGNCC 1 cut(s) 766
SchI GAGTC 3 cut(s) 569, 2004, 2137
ScrFI CCNGG 6 cut(s) 143, 159, 160, 422, 546, 894
SduI GDGCHC 2 cut(s) 443, 968
SfaNI GCATC 5 cut(s) 685, 799, 1136, 1213, 1626
SfcI CTRYAG 1 cut(s) 53
Sfr274I CTCGAG 1 cut(s) 1749
SfuI TTCGAA 2 cut(s) 1321, 2043
SinI GGWCC 1 cut(s) 766
SlaI CTCGAG 1 cut(s) 1749
SmaI CCCGGG 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 1749
SmoI CTYRAG 1 cut(s) 1749
SpeI ACTAGT 1 cut(s) 308
SsiI CCGC 7 cut(s) 175, 660, 914, 959, 1305, 1455, 2019
SspI AATATT 2 cut(s) 373, 460
SspMI CTAG 3 cut(s) 290, 309, 1484
StyD4I CCNGG 6 cut(s) 141, 157, 158, 420, 544, 892
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 6 cut(s) 107, 639, 694, 869, 1405, 1468
TaiI ACGT 2 cut(s) 524, 1034
TatI WGTACW 2 cut(s) 603, 1209
TauI GCSGC 4 cut(s) 663, 961, 1458, 2022
TfiI GAWTC 5 cut(s) 147, 392, 649, 1660, 1775
Tru1I TTAA 4 cut(s) 707, 929, 1397, 1551
Tru9I TTAA 4 cut(s) 707, 929, 1397, 1551
TscAI CASTG 6 cut(s) 448, 644, 785, 874, 1408, 2130
TseFI GTSAC 1 cut(s) 815
TseI GCWGC 7 cut(s) 55, 295, 417, 772, 1307, 1412, 1665
Tsp45I GTSAC 1 cut(s) 815
TspMI CCCGGG 1 cut(s) 158
TspRI CASTG 6 cut(s) 448, 644, 785, 874, 1408, 2130
VpaK11BI GGWCC 1 cut(s) 766
XapI RAATTY 8 cut(s) 4, 83, 186, 197, 384, 1525, 1547, 2059
XceI RCATGY 3 cut(s) 888, 1657, 1936
XhoI CTCGAG 1 cut(s) 1749
XmaI CCCGGG 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 2033
XspI CTAG 3 cut(s) 290, 309, 1484
Zsp2I ATGCAT 3 cut(s) 678, 1151, 1682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.