MuDR family transposase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 169 |
| AccB1I | GGYRCC | 1 cut(s) | 1208 |
| AccI | GTMKAC | 2 cut(s) | 1157, 1356 |
| AciI | CCGC | 2 cut(s) | 1186, 1400 |
| AclI | AACGTT | 2 cut(s) | 806, 1426 |
| AcuI | CTGAAG | 1 cut(s) | 171 |
| AdeI | CACNNNGTG | 1 cut(s) | 1066 |
| AfaI | GTAC | 1 cut(s) | 1676 |
| AfeI | AGCGCT | 1 cut(s) | 1012 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1802 |
| AflIII | ACRYGT | 1 cut(s) | 633 |
| AjnI | CCWGG | 2 cut(s) | 17, 1516 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 70, 102 |
| Alw21I | GWGCWC | 1 cut(s) | 234 |
| Alw26I | GTCTC | 1 cut(s) | 899 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 838 |
| Ama87I | CYCGRG | 1 cut(s) | 1798 |
| Aor51HI | AGCGCT | 1 cut(s) | 1012 |
| AoxI | GGCC | 4 cut(s) | 600, 1621, 1793, 1804 |
| ApeKI | GCWGC | 4 cut(s) | 1199, 1278, 1628, 1640 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 85, 1136 |
| AspA2I | CCTAGG | 1 cut(s) | 1807 |
| AspLEI | GCGC | 2 cut(s) | 224, 1013 |
| AspS9I | GGNCC | 5 cut(s) | 301, 462, 600, 1431, 1794 |
| AsuC2I | CCSGG | 3 cut(s) | 684, 1799, 1800 |
| AsuHPI | GGTGA | 1 cut(s) | 146 |
| AvaI | CYCGRG | 1 cut(s) | 1798 |
| AvaII | GGWCC | 3 cut(s) | 301, 462, 1431 |
| AvrII | CCTAGG | 1 cut(s) | 1807 |
| BanI | GGYRCC | 1 cut(s) | 1208 |
| BanII | GRGCYC | 1 cut(s) | 234 |
| BbsI | GAAGAC | 1 cut(s) | 1737 |
| Bbv12I | GWGCWC | 1 cut(s) | 234 |
| BbvI | GCAGC | 4 cut(s) | 1211, 1265, 1615, 1627 |
| BccI | CCATC | 5 cut(s) | 721, 794, 1390, 1471, 1728 |
| BceAI | ACGGC | 1 cut(s) | 1636 |
| BciT130I | CCWGG | 2 cut(s) | 19, 1518 |
| BciVI | GTATCC | 1 cut(s) | 15 |
| BcnI | CCSGG | 3 cut(s) | 684, 1799, 1800 |
| BcoDI | GTCTC | 1 cut(s) | 899 |
| BfaI | CTAG | 4 cut(s) | 32, 881, 1275, 1808 |
| BfmI | CTRYAG | 1 cut(s) | 1705 |
| BfoI | RGCGCY | 2 cut(s) | 225, 1014 |
| BfuI | GTATCC | 1 cut(s) | 15 |
| BisI | GCNGC | 4 cut(s) | 1200, 1279, 1629, 1641 |
| BlnI | CCTAGG | 1 cut(s) | 1807 |
| BlsI | GCNGC | 4 cut(s) | 1201, 1280, 1630, 1642 |
| BmcAI | AGTACT | 1 cut(s) | 1676 |
| Bme1390I | CCNGG | 5 cut(s) | 19, 684, 1518, 1799, 1800 |
| Bme18I | GGWCC | 3 cut(s) | 301, 462, 1431 |
| BmeT110I | CYCGRG | 1 cut(s) | 1798 |
| BmgT120I | GGNCC | 5 cut(s) | 301, 462, 600, 1431, 1794 |
| BmiI | GGNNCC | 4 cut(s) | 463, 957, 1210, 1796 |
| BmrFI | CCNGG | 5 cut(s) | 19, 684, 1518, 1799, 1800 |
| BmsI | GCATC | 7 cut(s) | 127, 361, 377, 604, 616, 901, 1338 |
| BpiI | GAAGAC | 1 cut(s) | 1737 |
| BpmI | CTGGAG | 2 cut(s) | 1224, 1539 |
| Bpu10I | CCTNAGC | 1 cut(s) | 245 |
| BpuEI | CTTGAG | 1 cut(s) | 945 |
| BpuMI | CCSGG | 3 cut(s) | 684, 1799, 1800 |
| BsaBI | GATNNNNATC | 1 cut(s) | 351 |
| BsaJI | CCNNGG | 7 cut(s) | 465, 521, 603, 682, 1797, 1798, 1807 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 70, 100, 744, 774, 1448, 1478 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1802 |
| Bse1I | ACTGG | 5 cut(s) | 785, 853, 923, 1080, 1352 |
| Bse3DI | GCAATG | 1 cut(s) | 483 |
| Bse8I | GATNNNNATC | 1 cut(s) | 351 |
| BseBI | CCWGG | 2 cut(s) | 19, 1518 |
| BseDI | CCNNGG | 7 cut(s) | 465, 521, 603, 682, 1797, 1798, 1807 |
| BseGI | GGATG | 1 cut(s) | 1720 |
| BseJI | GATNNNNATC | 1 cut(s) | 351 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1802 |
| BseMI | GCAATG | 1 cut(s) | 483 |
| BseMII | CTCAG | 2 cut(s) | 259, 278 |
| BseNI | ACTGG | 5 cut(s) | 785, 853, 923, 1080, 1352 |
| BseXI | GCAGC | 4 cut(s) | 1211, 1265, 1615, 1627 |
| BsgI | GTGCAG | 1 cut(s) | 1493 |
| BshFI | GGCC | 4 cut(s) | 602, 1623, 1795, 1806 |
| BshNI | GGYRCC | 1 cut(s) | 1208 |
| BsiHKAI | GWGCWC | 1 cut(s) | 234 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1798 |
| BsiSI | CCGG | 2 cut(s) | 684, 1799 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1802 |
| BsmAI | GTCTC | 1 cut(s) | 899 |
| BsmI | GAATGC | 1 cut(s) | 140 |
| BsnI | GGCC | 4 cut(s) | 602, 1623, 1795, 1806 |
| BsoBI | CYCGRG | 1 cut(s) | 1798 |
| Bsp1286I | GDGCHC | 1 cut(s) | 234 |
| Bsp143I | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| BspACI | CCGC | 2 cut(s) | 1186, 1400 |
| BspANI | GGCC | 4 cut(s) | 602, 1623, 1795, 1806 |
| BspCNI | CTCAG | 2 cut(s) | 258, 279 |
| BspLI | GGNNCC | 4 cut(s) | 463, 957, 1210, 1796 |
| BspQI | GCTCTTC | 2 cut(s) | 694, 1694 |
| BspT107I | GGYRCC | 1 cut(s) | 1208 |
| BsrDI | GCAATG | 1 cut(s) | 483 |
| BsrI | ACTGG | 5 cut(s) | 785, 853, 923, 1080, 1352 |
| BssECI | CCNNGG | 7 cut(s) | 465, 521, 603, 682, 1797, 1798, 1807 |
| BssMI | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| BssT1I | CCWWGG | 4 cut(s) | 465, 521, 603, 1807 |
| Bst2UI | CCWGG | 2 cut(s) | 19, 1518 |
| Bst4CI | ACNGT | 5 cut(s) | 86, 305, 1175, 1361, 1709 |
| Bst6I | CTCTTC | 4 cut(s) | 694, 938, 1694, 1784 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 320, 374, 1076 |
| BstDEI | CTNAG | 4 cut(s) | 245, 287, 742, 1193 |
| BstF5I | GGATG | 1 cut(s) | 1720 |
| BstH2I | RGCGCY | 2 cut(s) | 225, 1014 |
| BstHHI | GCGC | 2 cut(s) | 224, 1013 |
| BstKTI | GATC | 5 cut(s) | 298, 991, 1538, 1570, 1599 |
| BstMAI | GTCTC | 1 cut(s) | 899 |
| BstMBI | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 320, 374, 587, 608, 1076, 1208, 1348, 1637 |
| BstNI | CCWGG | 2 cut(s) | 19, 1518 |
| BstSCI | CCNGG | 5 cut(s) | 17, 682, 1516, 1797, 1798 |
| BstSFI | CTRYAG | 1 cut(s) | 1705 |
| BstV1I | GCAGC | 4 cut(s) | 1211, 1265, 1615, 1627 |
| BstV2I | GAAGAC | 1 cut(s) | 1737 |
| BsuI | GTATCC | 1 cut(s) | 15 |
| BsuRI | GGCC | 4 cut(s) | 602, 1623, 1795, 1806 |
| BtsCI | GGATG | 1 cut(s) | 1720 |
| BtsIMutI | CAGTG | 2 cut(s) | 792, 1504 |
| CaiI | CAGNNNCTG | 1 cut(s) | 838 |
| CfoI | GCGC | 2 cut(s) | 224, 1013 |
| Cfr13I | GGNCC | 5 cut(s) | 301, 462, 600, 1431, 1794 |
| Cfr9I | CCCGGG | 1 cut(s) | 1798 |
| CseI | GACGC | 1 cut(s) | 172 |
| Csp6I | GTAC | 1 cut(s) | 1675 |
| CviAII | CATG | 6 cut(s) | 218, 311, 359, 1571, 1581, 1682 |
| CviQI | GTAC | 1 cut(s) | 1675 |
| DdeI | CTNAG | 4 cut(s) | 245, 287, 742, 1193 |
| DpnI | GATC | 5 cut(s) | 297, 990, 1537, 1569, 1598 |
| DpnII | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| DraI | TTTAAA | 2 cut(s) | 57, 544 |
| DraIII | CACNNNGTG | 1 cut(s) | 1066 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 169 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 169 |
| Eam1104I | CTCTTC | 4 cut(s) | 694, 938, 1694, 1784 |
| EarI | CTCTTC | 4 cut(s) | 694, 938, 1694, 1784 |
| Ecl136II | GAGCTC | 1 cut(s) | 232 |
| Eco130I | CCWWGG | 4 cut(s) | 465, 521, 603, 1807 |
| Eco147I | AGGCCT | 1 cut(s) | 1806 |
| Eco24I | GRGCYC | 1 cut(s) | 234 |
| Eco47I | GGWCC | 3 cut(s) | 301, 462, 1431 |
| Eco47III | AGCGCT | 1 cut(s) | 1012 |
| Eco53kI | GAGCTC | 1 cut(s) | 232 |
| Eco57I | CTGAAG | 1 cut(s) | 171 |
| Eco88I | CYCGRG | 1 cut(s) | 1798 |
| EcoICRI | GAGCTC | 1 cut(s) | 232 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1794 |
| EcoRII | CCWGG | 2 cut(s) | 17, 1516 |
| EcoT14I | CCWWGG | 4 cut(s) | 465, 521, 603, 1807 |
| EcoT22I | ATGCAT | 1 cut(s) | 142 |
| EcoT38I | GRGCYC | 1 cut(s) | 234 |
| ErhI | CCWWGG | 4 cut(s) | 465, 521, 603, 1807 |
| FaeI | CATG | 6 cut(s) | 221, 314, 362, 1574, 1584, 1685 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 566, 598 |
| FatI | CATG | 6 cut(s) | 217, 310, 358, 1570, 1580, 1681 |
| FauI | CCCGC | 1 cut(s) | 1393 |
| FauNDI | CATATG | 2 cut(s) | 553, 1549 |
| FblI | GTMKAC | 2 cut(s) | 1157, 1356 |
| Fnu4HI | GCNGC | 4 cut(s) | 1200, 1279, 1629, 1641 |
| FokI | GGATG | 1 cut(s) | 1707 |
| FriOI | GRGCYC | 1 cut(s) | 234 |
| Fsp4HI | GCNGC | 4 cut(s) | 1200, 1279, 1629, 1641 |
| FspBI | CTAG | 4 cut(s) | 32, 881, 1275, 1808 |
| GlaI | GCGC | 2 cut(s) | 223, 1012 |
| GluI | GCNGC | 4 cut(s) | 1200, 1279, 1629, 1641 |
| GsuI | CTGGAG | 2 cut(s) | 1224, 1539 |
| HaeII | RGCGCY | 2 cut(s) | 225, 1014 |
| HaeIII | GGCC | 4 cut(s) | 602, 1623, 1795, 1806 |
| HapII | CCGG | 2 cut(s) | 684, 1799 |
| HgaI | GACGC | 1 cut(s) | 172 |
| HhaI | GCGC | 2 cut(s) | 224, 1013 |
| Hin1II | CATG | 6 cut(s) | 221, 314, 362, 1574, 1584, 1685 |
| Hin6I | GCGC | 2 cut(s) | 222, 1011 |
| HinP1I | GCGC | 2 cut(s) | 222, 1011 |
| HincII | GTYRAC | 1 cut(s) | 1558 |
| HindII | GTYRAC | 1 cut(s) | 1558 |
| HindIII | AAGCTT | 1 cut(s) | 452 |
| HinfI | GANTC | 7 cut(s) | 439, 835, 862, 899, 946, 1313, 1754 |
| HpaII | CCGG | 2 cut(s) | 684, 1799 |
| HphI | GGTGA | 1 cut(s) | 146 |
| Hpy166II | GTNNAC | 4 cut(s) | 157, 1158, 1357, 1558 |
| Hpy188III | TCNNGA | 6 cut(s) | 145, 247, 286, 299, 475, 1696 |
| Hpy8I | GTNNAC | 4 cut(s) | 157, 1158, 1357, 1558 |
| Hpy99I | CGWCG | 1 cut(s) | 1494 |
| HpyAV | CCTTC | 5 cut(s) | 332, 758, 846, 1159, 1462 |
| HpyCH4III | ACNGT | 5 cut(s) | 86, 305, 1175, 1361, 1709 |
| HpyCH4IV | ACGT | 3 cut(s) | 635, 806, 1426 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 320, 374, 587, 608, 1076, 1208, 1348, 1637 |
| HpyF3I | CTNAG | 4 cut(s) | 245, 287, 742, 1193 |
| HpySE526I | ACGT | 3 cut(s) | 635, 806, 1426 |
| Hsp92II | CATG | 6 cut(s) | 221, 314, 362, 1574, 1584, 1685 |
| HspAI | GCGC | 2 cut(s) | 222, 1011 |
| Kzo9I | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| LguI | GCTCTTC | 2 cut(s) | 694, 1694 |
| LmnI | GCTCC | 1 cut(s) | 237 |
| Lsp1109I | GCAGC | 4 cut(s) | 1211, 1265, 1615, 1627 |
| LweI | GCATC | 7 cut(s) | 127, 361, 377, 604, 616, 901, 1338 |
| MaeI | CTAG | 4 cut(s) | 32, 881, 1275, 1808 |
| MaeII | ACGT | 3 cut(s) | 635, 806, 1426 |
| MaeIII | GTNAC | 2 cut(s) | 677, 1052 |
| MalI | GATC | 5 cut(s) | 297, 990, 1537, 1569, 1598 |
| MboI | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| MfeI | CAATTG | 3 cut(s) | 258, 1335, 1600 |
| MhlI | GDGCHC | 1 cut(s) | 234 |
| MlyI | GAGTC | 2 cut(s) | 433, 955 |
| MmeI | TCCRAC | 4 cut(s) | 310, 681, 979, 1409 |
| Mph1103I | ATGCAT | 1 cut(s) | 142 |
| MroXI | GAANNNNTTC | 2 cut(s) | 85, 1136 |
| MseI | TTAA | 6 cut(s) | 56, 504, 543, 1320, 1383, 1587 |
| MslI | CAYNNNNRTG | 1 cut(s) | 357 |
| MspA1I | CMGCKG | 1 cut(s) | 1202 |
| MspI | CCGG | 2 cut(s) | 684, 1799 |
| MspR9I | CCNGG | 5 cut(s) | 19, 684, 1518, 1799, 1800 |
| MunI | CAATTG | 3 cut(s) | 258, 1335, 1600 |
| Mva1269I | GAATGC | 1 cut(s) | 140 |
| MvaI | CCWGG | 2 cut(s) | 19, 1518 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 320, 374, 587, 608, 1076, 1208, 1348, 1637 |
| NciI | CCSGG | 3 cut(s) | 684, 1799, 1800 |
| NdeI | CATATG | 2 cut(s) | 553, 1549 |
| NdeII | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| NlaIII | CATG | 6 cut(s) | 221, 314, 362, 1574, 1584, 1685 |
| NlaIV | GGNNCC | 4 cut(s) | 463, 957, 1210, 1796 |
| NsiI | ATGCAT | 1 cut(s) | 142 |
| PceI | AGGCCT | 1 cut(s) | 1806 |
| PciSI | GCTCTTC | 2 cut(s) | 694, 1694 |
| PctI | GAATGC | 1 cut(s) | 140 |
| PdmI | GAANNNNTTC | 2 cut(s) | 85, 1136 |
| PfeI | GAWTC | 5 cut(s) | 835, 862, 899, 1313, 1754 |
| PfoI | TCCNGGA | 1 cut(s) | 1516 |
| PkrI | GCNGC | 4 cut(s) | 1201, 1280, 1630, 1642 |
| PleI | GAGTC | 2 cut(s) | 433, 954 |
| PpsI | GAGTC | 2 cut(s) | 433, 954 |
| Psp124BI | GAGCTC | 1 cut(s) | 234 |
| Psp1406I | AACGTT | 2 cut(s) | 806, 1426 |
| Psp6I | CCWGG | 2 cut(s) | 17, 1516 |
| PspGI | CCWGG | 2 cut(s) | 17, 1516 |
| PspN4I | GGNNCC | 4 cut(s) | 463, 957, 1210, 1796 |
| PspPI | GGNCC | 5 cut(s) | 301, 462, 600, 1431, 1794 |
| PstNI | CAGNNNCTG | 1 cut(s) | 838 |
| PvuII | CAGCTG | 1 cut(s) | 1202 |
| RsaI | GTAC | 1 cut(s) | 1676 |
| RsaNI | GTAC | 1 cut(s) | 1675 |
| RseI | CAYNNNNRTG | 1 cut(s) | 357 |
| SacI | GAGCTC | 1 cut(s) | 234 |
| SapI | GCTCTTC | 2 cut(s) | 694, 1694 |
| SaqAI | TTAA | 6 cut(s) | 56, 504, 543, 1320, 1383, 1587 |
| SatI | GCNGC | 4 cut(s) | 1200, 1279, 1629, 1641 |
| Sau3AI | GATC | 5 cut(s) | 295, 988, 1535, 1567, 1596 |
| Sau96I | GGNCC | 5 cut(s) | 301, 462, 600, 1431, 1794 |
| ScaI | AGTACT | 1 cut(s) | 1676 |
| SchI | GAGTC | 2 cut(s) | 433, 955 |
| ScrFI | CCNGG | 5 cut(s) | 19, 684, 1518, 1799, 1800 |
| SduI | GDGCHC | 1 cut(s) | 234 |
| SfaNI | GCATC | 7 cut(s) | 127, 361, 377, 604, 616, 901, 1338 |
| SfcI | CTRYAG | 1 cut(s) | 1705 |
| SinI | GGWCC | 3 cut(s) | 301, 462, 1431 |
| SmaI | CCCGGG | 1 cut(s) | 1800 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 357 |
| SmlI | CTYRAG | 1 cut(s) | 960 |
| SmoI | CTYRAG | 1 cut(s) | 960 |
| SseBI | AGGCCT | 1 cut(s) | 1806 |
| SsiI | CCGC | 2 cut(s) | 1186, 1400 |
| SspMI | CTAG | 4 cut(s) | 32, 881, 1275, 1808 |
| SstI | GAGCTC | 1 cut(s) | 234 |
| StuI | AGGCCT | 1 cut(s) | 1806 |
| StyD4I | CCNGG | 5 cut(s) | 17, 682, 1516, 1797, 1798 |
| StyI | CCWWGG | 4 cut(s) | 465, 521, 603, 1807 |
| TaaI | ACNGT | 5 cut(s) | 86, 305, 1175, 1361, 1709 |
| TaiI | ACGT | 3 cut(s) | 638, 809, 1429 |
| TaqI | TCGA | 4 cut(s) | 42, 273, 1017, 1534 |
| TatI | WGTACW | 1 cut(s) | 1674 |
| TfiI | GAWTC | 5 cut(s) | 835, 862, 899, 1313, 1754 |
| Tru1I | TTAA | 6 cut(s) | 56, 504, 543, 1320, 1383, 1587 |
| Tru9I | TTAA | 6 cut(s) | 56, 504, 543, 1320, 1383, 1587 |
| TscAI | CASTG | 2 cut(s) | 792, 1504 |
| TseI | GCWGC | 4 cut(s) | 1199, 1278, 1628, 1640 |
| TspDTI | ATGAA | 9 cut(s) | 132, 206, 299, 347, 501, 558, 1536, 1569, 1670 |
| TspGWI | ACGGA | 3 cut(s) | 224, 350, 765 |
| TspMI | CCCGGG | 1 cut(s) | 1798 |
| TspRI | CASTG | 2 cut(s) | 792, 1504 |
| VpaK11BI | GGWCC | 3 cut(s) | 301, 462, 1431 |
| XmaI | CCCGGG | 1 cut(s) | 1798 |
| XmaJI | CCTAGG | 1 cut(s) | 1807 |
| XmiI | GTMKAC | 2 cut(s) | 1157, 1356 |
| XmnI | GAANNNNTTC | 2 cut(s) | 85, 1136 |
| XspI | CTAG | 4 cut(s) | 32, 881, 1275, 1808 |
| ZrmI | AGTACT | 1 cut(s) | 1676 |
| Zsp2I | ATGCAT | 1 cut(s) | 142 |