MuDR family transposase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 98, 147 |
| AccI | GTMKAC | 3 cut(s) | 47, 246, 808 |
| AciI | CCGC | 3 cut(s) | 76, 290, 709 |
| AclI | AACGTT | 1 cut(s) | 316 |
| AcsI | RAATTY | 2 cut(s) | 761, 799 |
| AfaI | GTAC | 2 cut(s) | 566, 741 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 693 |
| AgsI | TTSAA | 5 cut(s) | 70, 358, 402, 586, 715 |
| AjnI | CCWGG | 2 cut(s) | 406, 688 |
| AluBI | AGCT | 3 cut(s) | 92, 168, 594 |
| AluI | AGCT | 3 cut(s) | 92, 168, 594 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 536, 653 |
| AoxI | GGCC | 4 cut(s) | 511, 683, 695, 732 |
| ApeKI | GCWGC | 3 cut(s) | 168, 518, 530 |
| ApoI | RAATTY | 2 cut(s) | 761, 799 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 710 |
| AspA2I | CCTAGG | 1 cut(s) | 698 |
| AspS9I | GGNCC | 2 cut(s) | 321, 684 |
| AsuHPI | GGTGA | 1 cut(s) | 715 |
| AvaII | GGWCC | 1 cut(s) | 321 |
| AvrII | CCTAGG | 1 cut(s) | 698 |
| BanI | GGYRCC | 2 cut(s) | 98, 147 |
| BbsI | GAAGAC | 1 cut(s) | 627 |
| BbvI | GCAGC | 3 cut(s) | 155, 505, 542 |
| BccI | CCATC | 3 cut(s) | 280, 361, 618 |
| BceAI | ACGGC | 2 cut(s) | 135, 526 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 27, 61 |
| BciT130I | CCWGG | 2 cut(s) | 408, 690 |
| BfaI | CTAG | 2 cut(s) | 165, 699 |
| BfmI | CTRYAG | 1 cut(s) | 247 |
| BisI | GCNGC | 3 cut(s) | 169, 519, 531 |
| BlnI | CCTAGG | 1 cut(s) | 698 |
| BlsI | GCNGC | 3 cut(s) | 170, 520, 532 |
| BmcAI | AGTACT | 1 cut(s) | 566 |
| Bme1390I | CCNGG | 2 cut(s) | 408, 690 |
| Bme18I | GGWCC | 1 cut(s) | 321 |
| BmgT120I | GGNCC | 2 cut(s) | 321, 684 |
| BmiI | GGNNCC | 3 cut(s) | 100, 149, 686 |
| BmrFI | CCNGG | 2 cut(s) | 408, 690 |
| BmsI | GCATC | 1 cut(s) | 228 |
| BpiI | GAAGAC | 1 cut(s) | 627 |
| BpmI | CTGGAG | 2 cut(s) | 114, 429 |
| BsaJI | CCNNGG | 3 cut(s) | 688, 689, 698 |
| BsaWI | WCCGGW | 1 cut(s) | 765 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 338, 368 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 693 |
| BseBI | CCWGG | 2 cut(s) | 408, 690 |
| BseDI | CCNNGG | 3 cut(s) | 688, 689, 698 |
| BseGI | GGATG | 1 cut(s) | 610 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 693 |
| BseMII | CTCAG | 3 cut(s) | 65, 750, 771 |
| BseXI | GCAGC | 3 cut(s) | 155, 505, 542 |
| BsgI | GTGCAG | 2 cut(s) | 383, 803 |
| BshFI | GGCC | 4 cut(s) | 513, 685, 697, 734 |
| BshNI | GGYRCC | 2 cut(s) | 98, 147 |
| BsiSI | CCGG | 1 cut(s) | 766 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 693 |
| BsnI | GGCC | 4 cut(s) | 513, 685, 697, 734 |
| Bsp143I | GATC | 3 cut(s) | 425, 457, 486 |
| BspACI | CCGC | 3 cut(s) | 76, 290, 709 |
| BspANI | GGCC | 4 cut(s) | 513, 685, 697, 734 |
| BspCNI | CTCAG | 3 cut(s) | 64, 749, 772 |
| BspLI | GGNNCC | 3 cut(s) | 100, 149, 686 |
| BspQI | GCTCTTC | 1 cut(s) | 584 |
| BspT107I | GGYRCC | 2 cut(s) | 98, 147 |
| BssECI | CCNNGG | 3 cut(s) | 688, 689, 698 |
| BssMI | GATC | 3 cut(s) | 425, 457, 486 |
| BssT1I | CCWWGG | 1 cut(s) | 698 |
| Bst2UI | CCWGG | 2 cut(s) | 408, 690 |
| Bst4CI | ACNGT | 3 cut(s) | 65, 251, 744 |
| Bst6I | CTCTTC | 2 cut(s) | 584, 674 |
| BstDEI | CTNAG | 4 cut(s) | 51, 83, 736, 780 |
| BstF5I | GGATG | 1 cut(s) | 610 |
| BstKTI | GATC | 3 cut(s) | 428, 460, 489 |
| BstMBI | GATC | 3 cut(s) | 425, 457, 486 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 98, 238, 527 |
| BstNI | CCWGG | 2 cut(s) | 408, 690 |
| BstSCI | CCNGG | 2 cut(s) | 406, 688 |
| BstSFI | CTRYAG | 1 cut(s) | 247 |
| BstV1I | GCAGC | 3 cut(s) | 155, 505, 542 |
| BstV2I | GAAGAC | 1 cut(s) | 627 |
| BsuRI | GGCC | 4 cut(s) | 513, 685, 697, 734 |
| BtsCI | GGATG | 1 cut(s) | 610 |
| BtsIMutI | CAGTG | 1 cut(s) | 394 |
| CaiI | CAGNNNCTG | 2 cut(s) | 536, 653 |
| Cfr13I | GGNCC | 2 cut(s) | 321, 684 |
| Csp6I | GTAC | 2 cut(s) | 565, 740 |
| CviAII | CATG | 3 cut(s) | 461, 471, 572 |
| CviQI | GTAC | 2 cut(s) | 565, 740 |
| DdeI | CTNAG | 4 cut(s) | 51, 83, 736, 780 |
| DpnI | GATC | 3 cut(s) | 427, 459, 488 |
| DpnII | GATC | 3 cut(s) | 425, 457, 486 |
| Eam1104I | CTCTTC | 2 cut(s) | 584, 674 |
| EarI | CTCTTC | 2 cut(s) | 584, 674 |
| Eco130I | CCWWGG | 1 cut(s) | 698 |
| Eco147I | AGGCCT | 1 cut(s) | 697 |
| Eco47I | GGWCC | 1 cut(s) | 321 |
| EcoO109I | RGGNCCY | 1 cut(s) | 684 |
| EcoRI | GAATTC | 1 cut(s) | 761 |
| EcoRII | CCWGG | 2 cut(s) | 406, 688 |
| EcoT14I | CCWWGG | 1 cut(s) | 698 |
| ErhI | CCWWGG | 1 cut(s) | 698 |
| FaeI | CATG | 3 cut(s) | 464, 474, 575 |
| FatI | CATG | 3 cut(s) | 460, 470, 571 |
| FauI | CCCGC | 1 cut(s) | 283 |
| FauNDI | CATATG | 1 cut(s) | 439 |
| FblI | GTMKAC | 3 cut(s) | 47, 246, 808 |
| Fnu4HI | GCNGC | 3 cut(s) | 169, 519, 531 |
| FokI | GGATG | 1 cut(s) | 597 |
| Fsp4HI | GCNGC | 3 cut(s) | 169, 519, 531 |
| FspBI | CTAG | 2 cut(s) | 165, 699 |
| GluI | GCNGC | 3 cut(s) | 169, 519, 531 |
| GsuI | CTGGAG | 2 cut(s) | 114, 429 |
| HaeIII | GGCC | 4 cut(s) | 513, 685, 697, 734 |
| HapII | CCGG | 1 cut(s) | 766 |
| Hin1II | CATG | 3 cut(s) | 464, 474, 575 |
| HincII | GTYRAC | 2 cut(s) | 48, 448 |
| HindII | GTYRAC | 2 cut(s) | 48, 448 |
| HinfI | GANTC | 4 cut(s) | 49, 203, 644, 782 |
| HpaII | CCGG | 1 cut(s) | 766 |
| HphI | GGTGA | 1 cut(s) | 715 |
| Hpy166II | GTNNAC | 4 cut(s) | 48, 247, 448, 809 |
| Hpy188III | TCNNGA | 1 cut(s) | 586 |
| Hpy8I | GTNNAC | 4 cut(s) | 48, 247, 448, 809 |
| Hpy99I | CGWCG | 2 cut(s) | 49, 384 |
| HpyAV | CCTTC | 2 cut(s) | 49, 352 |
| HpyCH4III | ACNGT | 3 cut(s) | 65, 251, 744 |
| HpyCH4IV | ACGT | 1 cut(s) | 316 |
| HpyCH4V | TGCA | 5 cut(s) | 155, 224, 364, 521, 820 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 98, 238, 527 |
| HpyF3I | CTNAG | 4 cut(s) | 51, 83, 736, 780 |
| HpySE526I | ACGT | 1 cut(s) | 316 |
| Hsp92II | CATG | 3 cut(s) | 464, 474, 575 |
| Kzo9I | GATC | 3 cut(s) | 425, 457, 486 |
| LguI | GCTCTTC | 1 cut(s) | 584 |
| LpnPI | CCDG | 8 cut(s) | 78, 147, 275, 393, 420, 675, 702, 779 |
| Lsp1109I | GCAGC | 3 cut(s) | 155, 505, 542 |
| LweI | GCATC | 1 cut(s) | 228 |
| MaeI | CTAG | 2 cut(s) | 165, 699 |
| MaeII | ACGT | 1 cut(s) | 316 |
| MaeIII | GTNAC | 1 cut(s) | 783 |
| MalI | GATC | 3 cut(s) | 427, 459, 488 |
| MboI | GATC | 3 cut(s) | 425, 457, 486 |
| MboII | GAAGA | 7 cut(s) | 20, 317, 601, 627, 630, 691, 765 |
| MfeI | CAATTG | 2 cut(s) | 225, 490 |
| MlyI | GAGTC | 2 cut(s) | 43, 791 |
| MmeI | TCCRAC | 1 cut(s) | 299 |
| MroXI | GAANNNNTTC | 1 cut(s) | 710 |
| MseI | TTAA | 5 cut(s) | 30, 210, 273, 477, 725 |
| MslI | CAYNNNNRTG | 1 cut(s) | 794 |
| MspI | CCGG | 1 cut(s) | 766 |
| MspR9I | CCNGG | 2 cut(s) | 408, 690 |
| MunI | CAATTG | 2 cut(s) | 225, 490 |
| MvaI | CCWGG | 2 cut(s) | 408, 690 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 98, 238, 527 |
| NdeI | CATATG | 1 cut(s) | 439 |
| NdeII | GATC | 3 cut(s) | 425, 457, 486 |
| NlaIII | CATG | 3 cut(s) | 464, 474, 575 |
| NlaIV | GGNNCC | 3 cut(s) | 100, 149, 686 |
| NmuCI | GTSAC | 1 cut(s) | 783 |
| PasI | CCCWGGG | 1 cut(s) | 689 |
| PceI | AGGCCT | 1 cut(s) | 697 |
| PciSI | GCTCTTC | 1 cut(s) | 584 |
| PdmI | GAANNNNTTC | 1 cut(s) | 710 |
| PfeI | GAWTC | 2 cut(s) | 203, 644 |
| PfoI | TCCNGGA | 1 cut(s) | 406 |
| PkrI | GCNGC | 3 cut(s) | 170, 520, 532 |
| PleI | GAGTC | 2 cut(s) | 43, 790 |
| PpsI | GAGTC | 2 cut(s) | 43, 790 |
| Psp1406I | AACGTT | 1 cut(s) | 316 |
| Psp6I | CCWGG | 2 cut(s) | 406, 688 |
| PspGI | CCWGG | 2 cut(s) | 406, 688 |
| PspN4I | GGNNCC | 3 cut(s) | 100, 149, 686 |
| PspPI | GGNCC | 2 cut(s) | 321, 684 |
| PstNI | CAGNNNCTG | 2 cut(s) | 536, 653 |
| RsaI | GTAC | 2 cut(s) | 566, 741 |
| RsaNI | GTAC | 2 cut(s) | 565, 740 |
| RseI | CAYNNNNRTG | 1 cut(s) | 794 |
| SalI | GTCGAC | 1 cut(s) | 46 |
| SapI | GCTCTTC | 1 cut(s) | 584 |
| SaqAI | TTAA | 5 cut(s) | 30, 210, 273, 477, 725 |
| SatI | GCNGC | 3 cut(s) | 169, 519, 531 |
| Sau3AI | GATC | 3 cut(s) | 425, 457, 486 |
| Sau96I | GGNCC | 2 cut(s) | 321, 684 |
| ScaI | AGTACT | 1 cut(s) | 566 |
| SchI | GAGTC | 2 cut(s) | 43, 791 |
| ScrFI | CCNGG | 2 cut(s) | 408, 690 |
| SfaNI | GCATC | 1 cut(s) | 228 |
| SfcI | CTRYAG | 1 cut(s) | 247 |
| SinI | GGWCC | 1 cut(s) | 321 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 794 |
| SseBI | AGGCCT | 1 cut(s) | 697 |
| SsiI | CCGC | 3 cut(s) | 76, 290, 709 |
| SspMI | CTAG | 2 cut(s) | 165, 699 |
| StuI | AGGCCT | 1 cut(s) | 697 |
| StyD4I | CCNGG | 2 cut(s) | 406, 688 |
| StyI | CCWWGG | 1 cut(s) | 698 |
| TaaI | ACNGT | 3 cut(s) | 65, 251, 744 |
| TaiI | ACGT | 1 cut(s) | 319 |
| TaqI | TCGA | 2 cut(s) | 47, 424 |
| TatI | WGTACW | 2 cut(s) | 564, 739 |
| TfiI | GAWTC | 2 cut(s) | 203, 644 |
| Tru1I | TTAA | 5 cut(s) | 30, 210, 273, 477, 725 |
| Tru9I | TTAA | 5 cut(s) | 30, 210, 273, 477, 725 |
| TscAI | CASTG | 1 cut(s) | 394 |
| TseFI | GTSAC | 1 cut(s) | 783 |
| TseI | GCWGC | 3 cut(s) | 168, 518, 530 |
| Tsp45I | GTSAC | 1 cut(s) | 783 |
| TspDTI | ATGAA | 3 cut(s) | 426, 459, 560 |
| TspRI | CASTG | 1 cut(s) | 394 |
| VpaK11BI | GGWCC | 1 cut(s) | 321 |
| XapI | RAATTY | 2 cut(s) | 761, 799 |
| XmaJI | CCTAGG | 1 cut(s) | 698 |
| XmiI | GTMKAC | 3 cut(s) | 47, 246, 808 |
| XmnI | GAANNNNTTC | 1 cut(s) | 710 |
| XspI | CTAG | 2 cut(s) | 165, 699 |
| ZrmI | AGTACT | 1 cut(s) | 566 |