MuDR family transposase
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 93, 906 |
| AccI | GTMKAC | 1 cut(s) | 527 |
| AciI | CCGC | 1 cut(s) | 368 |
| AclWI | GGATC | 2 cut(s) | 759, 851 |
| AcoI | YGGCCR | 1 cut(s) | 901 |
| AcsI | RAATTY | 1 cut(s) | 209 |
| AdeI | CACNNNGTG | 3 cut(s) | 145, 888, 890 |
| AfaI | GTAC | 1 cut(s) | 553 |
| AgsI | TTSAA | 8 cut(s) | 97, 193, 337, 386, 434, 545, 647, 689 |
| AluBI | AGCT | 5 cut(s) | 17, 164, 333, 469, 791 |
| AluI | AGCT | 5 cut(s) | 17, 164, 333, 469, 791 |
| Alw21I | GWGCWC | 1 cut(s) | 724 |
| AlwI | GGATC | 2 cut(s) | 759, 851 |
| AoxI | GGCC | 1 cut(s) | 901 |
| ApeKI | GCWGC | 3 cut(s) | 164, 370, 796 |
| ApoI | RAATTY | 1 cut(s) | 209 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 390 |
| AspLEI | GCGC | 1 cut(s) | 58 |
| AsuC2I | CCSGG | 2 cut(s) | 749, 822 |
| AsuHPI | GGTGA | 3 cut(s) | 317, 586, 794 |
| BalI | TGGCCA | 1 cut(s) | 903 |
| BbsI | GAAGAC | 2 cut(s) | 707, 832 |
| Bbv12I | GWGCWC | 1 cut(s) | 724 |
| BbvI | GCAGC | 3 cut(s) | 151, 357, 808 |
| BccI | CCATC | 1 cut(s) | 23 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 254, 288 |
| BciVI | GTATCC | 2 cut(s) | 117, 680 |
| BclI | TGATCA | 1 cut(s) | 892 |
| BcnI | CCSGG | 2 cut(s) | 749, 822 |
| BfaI | CTAG | 2 cut(s) | 174, 771 |
| BfmI | CTRYAG | 1 cut(s) | 712 |
| BfuAI | ACCTGC | 2 cut(s) | 93, 906 |
| BfuI | GTATCC | 2 cut(s) | 117, 680 |
| BisI | GCNGC | 4 cut(s) | 165, 368, 371, 797 |
| BlsI | GCNGC | 4 cut(s) | 166, 369, 372, 798 |
| Bme1390I | CCNGG | 2 cut(s) | 749, 822 |
| BmrFI | CCNGG | 2 cut(s) | 749, 822 |
| BmsI | GCATC | 2 cut(s) | 88, 350 |
| BpiI | GAAGAC | 2 cut(s) | 707, 832 |
| Bpu10I | CCTNAGC | 1 cut(s) | 465 |
| BpuEI | CTTGAG | 1 cut(s) | 491 |
| BpuMI | CCSGG | 2 cut(s) | 749, 822 |
| BsaJI | CCNNGG | 2 cut(s) | 10, 830 |
| Bse1I | ACTGG | 4 cut(s) | 523, 701, 743, 872 |
| Bse3DI | GCAATG | 2 cut(s) | 571, 931 |
| BseDI | CCNNGG | 2 cut(s) | 10, 830 |
| BseGI | GGATG | 2 cut(s) | 34, 358 |
| BseMI | GCAATG | 2 cut(s) | 571, 931 |
| BseMII | CTCAG | 1 cut(s) | 389 |
| BseNI | ACTGG | 4 cut(s) | 523, 701, 743, 872 |
| BseRI | GAGGAG | 1 cut(s) | 463 |
| BseXI | GCAGC | 3 cut(s) | 151, 357, 808 |
| BshFI | GGCC | 1 cut(s) | 903 |
| BsiHKAI | GWGCWC | 1 cut(s) | 724 |
| BsiSI | CCGG | 2 cut(s) | 749, 822 |
| BslFI | GGGAC | 1 cut(s) | 731 |
| BsmFI | GGGAC | 1 cut(s) | 731 |
| BsmI | GAATGC | 1 cut(s) | 390 |
| BsnI | GGCC | 1 cut(s) | 903 |
| Bsp1286I | GDGCHC | 1 cut(s) | 724 |
| Bsp1407I | TGTACA | 1 cut(s) | 551 |
| Bsp143I | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| BspACI | CCGC | 1 cut(s) | 368 |
| BspANI | GGCC | 1 cut(s) | 903 |
| BspCNI | CTCAG | 1 cut(s) | 388 |
| BspHI | TCATGA | 1 cut(s) | 724 |
| BspMI | ACCTGC | 2 cut(s) | 93, 906 |
| BspPI | GGATC | 2 cut(s) | 759, 851 |
| BsrDI | GCAATG | 2 cut(s) | 571, 931 |
| BsrGI | TGTACA | 1 cut(s) | 551 |
| BsrI | ACTGG | 4 cut(s) | 523, 701, 743, 872 |
| BssECI | CCNNGG | 2 cut(s) | 10, 830 |
| BssMI | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| Bst4CI | ACNGT | 5 cut(s) | 11, 71, 668, 680, 888 |
| Bst6I | CTCTTC | 1 cut(s) | 855 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 921 |
| BstAUI | TGTACA | 1 cut(s) | 551 |
| BstC8I | GCNNGC | 2 cut(s) | 283, 365 |
| BstDEI | CTNAG | 2 cut(s) | 375, 465 |
| BstDSI | CCRYGG | 2 cut(s) | 10, 830 |
| BstF5I | GGATG | 2 cut(s) | 34, 358 |
| BstHHI | GCGC | 1 cut(s) | 58 |
| BstKTI | GATC | 5 cut(s) | 730, 754, 846, 895, 936 |
| BstMBI | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 170, 379, 921 |
| BstNSI | RCATGY | 1 cut(s) | 367 |
| BstSCI | CCNGG | 2 cut(s) | 747, 820 |
| BstSFI | CTRYAG | 1 cut(s) | 712 |
| BstV1I | GCAGC | 3 cut(s) | 151, 357, 808 |
| BstV2I | GAAGAC | 2 cut(s) | 707, 832 |
| BstX2I | RGATCY | 1 cut(s) | 751 |
| BstYI | RGATCY | 1 cut(s) | 751 |
| BsuI | GTATCC | 2 cut(s) | 117, 680 |
| BsuRI | GGCC | 1 cut(s) | 903 |
| BtgI | CCRYGG | 2 cut(s) | 10, 830 |
| BtsCI | GGATG | 2 cut(s) | 34, 358 |
| BtsIMutI | CAGTG | 1 cut(s) | 879 |
| BveI | ACCTGC | 2 cut(s) | 93, 906 |
| Cac8I | GCNNGC | 2 cut(s) | 283, 365 |
| CciI | TCATGA | 1 cut(s) | 724 |
| CfoI | GCGC | 1 cut(s) | 58 |
| Csp6I | GTAC | 1 cut(s) | 552 |
| CviAII | CATG | 7 cut(s) | 343, 364, 571, 630, 638, 725, 921 |
| CviJI | RGCY | 9 cut(s) | 17, 164, 333, 469, 522, 763, 791, 799, 903 |
| CviKI_1 | RGCY | 9 cut(s) | 17, 164, 333, 469, 522, 763, 791, 799, 903 |
| CviQI | GTAC | 1 cut(s) | 552 |
| DdeI | CTNAG | 2 cut(s) | 375, 465 |
| DpnI | GATC | 5 cut(s) | 729, 753, 845, 894, 935 |
| DpnII | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| DraIII | CACNNNGTG | 3 cut(s) | 145, 888, 890 |
| EaeI | YGGCCR | 1 cut(s) | 901 |
| Eam1104I | CTCTTC | 1 cut(s) | 855 |
| EarI | CTCTTC | 1 cut(s) | 855 |
| Eco32I | GATATC | 1 cut(s) | 187 |
| EcoRV | GATATC | 1 cut(s) | 187 |
| EcoT22I | ATGCAT | 1 cut(s) | 365 |
| FaeI | CATG | 7 cut(s) | 346, 367, 574, 633, 641, 728, 924 |
| FaqI | GGGAC | 1 cut(s) | 731 |
| FatI | CATG | 7 cut(s) | 342, 363, 570, 629, 637, 724, 920 |
| FauNDI | CATATG | 1 cut(s) | 169 |
| FbaI | TGATCA | 1 cut(s) | 892 |
| FblI | GTMKAC | 1 cut(s) | 527 |
| Fnu4HI | GCNGC | 4 cut(s) | 165, 368, 371, 797 |
| FokI | GGATG | 2 cut(s) | 41, 365 |
| Fsp4HI | GCNGC | 4 cut(s) | 165, 368, 371, 797 |
| FspBI | CTAG | 2 cut(s) | 174, 771 |
| GlaI | GCGC | 1 cut(s) | 57 |
| GluI | GCNGC | 4 cut(s) | 165, 368, 371, 797 |
| HaeIII | GGCC | 1 cut(s) | 903 |
| HapII | CCGG | 2 cut(s) | 749, 822 |
| HhaI | GCGC | 1 cut(s) | 58 |
| Hin1II | CATG | 7 cut(s) | 346, 367, 574, 633, 641, 728, 924 |
| Hin6I | GCGC | 1 cut(s) | 56 |
| HinP1I | GCGC | 1 cut(s) | 56 |
| HindIII | AAGCTT | 1 cut(s) | 467 |
| HinfI | GANTC | 3 cut(s) | 785, 808, 863 |
| HpaII | CCGG | 2 cut(s) | 749, 822 |
| HphI | GGTGA | 3 cut(s) | 317, 586, 794 |
| Hpy166II | GTNNAC | 3 cut(s) | 156, 528, 577 |
| Hpy188I | TCNGA | 1 cut(s) | 938 |
| Hpy188III | TCNNGA | 3 cut(s) | 725, 755, 867 |
| Hpy8I | GTNNAC | 3 cut(s) | 156, 528, 577 |
| HpyAV | CCTTC | 3 cut(s) | 380, 421, 652 |
| HpyCH4III | ACNGT | 5 cut(s) | 11, 71, 668, 680, 888 |
| HpyCH4V | TGCA | 7 cut(s) | 167, 363, 641, 655, 796, 882, 924 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 170, 379, 921 |
| HpyF3I | CTNAG | 2 cut(s) | 375, 465 |
| Hsp92II | CATG | 7 cut(s) | 346, 367, 574, 633, 641, 728, 924 |
| HspAI | GCGC | 1 cut(s) | 56 |
| Ksp22I | TGATCA | 1 cut(s) | 892 |
| Kzo9I | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| LmnI | GCTCC | 1 cut(s) | 14 |
| Lsp1109I | GCAGC | 3 cut(s) | 151, 357, 808 |
| LweI | GCATC | 2 cut(s) | 88, 350 |
| MaeI | CTAG | 2 cut(s) | 174, 771 |
| MaeIII | GTNAC | 1 cut(s) | 680 |
| MalI | GATC | 5 cut(s) | 729, 753, 845, 894, 935 |
| MboI | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| MboII | GAAGA | 8 cut(s) | 181, 218, 429, 701, 712, 722, 837, 872 |
| MflI | RGATCY | 1 cut(s) | 751 |
| MhlI | GDGCHC | 1 cut(s) | 724 |
| MlsI | TGGCCA | 1 cut(s) | 903 |
| MluCI | AATT | 4 cut(s) | 91, 209, 316, 508 |
| MluNI | TGGCCA | 1 cut(s) | 903 |
| MlyI | GAGTC | 2 cut(s) | 802, 872 |
| MmeI | TCCRAC | 1 cut(s) | 183 |
| Mox20I | TGGCCA | 1 cut(s) | 903 |
| Mph1103I | ATGCAT | 1 cut(s) | 365 |
| MroXI | GAANNNNTTC | 1 cut(s) | 390 |
| MscI | TGGCCA | 1 cut(s) | 903 |
| MseI | TTAA | 1 cut(s) | 132 |
| MslI | CAYNNNNRTG | 1 cut(s) | 395 |
| Msp20I | TGGCCA | 1 cut(s) | 903 |
| MspA1I | CMGCKG | 1 cut(s) | 370 |
| MspI | CCGG | 2 cut(s) | 749, 822 |
| MspR9I | CCNGG | 2 cut(s) | 749, 822 |
| Mva1269I | GAATGC | 1 cut(s) | 390 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 170, 379, 921 |
| NciI | CCSGG | 2 cut(s) | 749, 822 |
| NdeI | CATATG | 1 cut(s) | 169 |
| NdeII | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| NlaIII | CATG | 7 cut(s) | 346, 367, 574, 633, 641, 728, 924 |
| NsiI | ATGCAT | 1 cut(s) | 365 |
| NspI | RCATGY | 1 cut(s) | 367 |
| PaeI | GCATGC | 1 cut(s) | 367 |
| PagI | TCATGA | 1 cut(s) | 724 |
| PctI | GAATGC | 1 cut(s) | 390 |
| PdmI | GAANNNNTTC | 1 cut(s) | 390 |
| PfeI | GAWTC | 1 cut(s) | 785 |
| PfoI | TCCNGGA | 2 cut(s) | 747, 820 |
| PkrI | GCNGC | 4 cut(s) | 166, 369, 372, 798 |
| PleI | GAGTC | 2 cut(s) | 802, 871 |
| PpsI | GAGTC | 2 cut(s) | 802, 871 |
| PsuI | RGATCY | 1 cut(s) | 751 |
| RsaI | GTAC | 1 cut(s) | 553 |
| RsaNI | GTAC | 1 cut(s) | 552 |
| RseI | CAYNNNNRTG | 1 cut(s) | 395 |
| SaqAI | TTAA | 1 cut(s) | 132 |
| SatI | GCNGC | 4 cut(s) | 165, 368, 371, 797 |
| Sau3AI | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| SchI | GAGTC | 2 cut(s) | 802, 872 |
| ScrFI | CCNGG | 2 cut(s) | 749, 822 |
| SduI | GDGCHC | 1 cut(s) | 724 |
| SfaNI | GCATC | 2 cut(s) | 88, 350 |
| SfcI | CTRYAG | 1 cut(s) | 712 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 395 |
| SmlI | CTYRAG | 1 cut(s) | 470 |
| SmoI | CTYRAG | 1 cut(s) | 470 |
| SphI | GCATGC | 1 cut(s) | 367 |
| Sse9I | AATT | 4 cut(s) | 91, 209, 316, 508 |
| SsiI | CCGC | 1 cut(s) | 368 |
| SspMI | CTAG | 2 cut(s) | 174, 771 |
| StyD4I | CCNGG | 2 cut(s) | 747, 820 |
| TaaI | ACNGT | 5 cut(s) | 11, 71, 668, 680, 888 |
| TaqI | TCGA | 2 cut(s) | 485, 811 |
| TasI | AATT | 4 cut(s) | 91, 209, 316, 508 |
| TatI | WGTACW | 1 cut(s) | 551 |
| TauI | GCSGC | 1 cut(s) | 370 |
| TfiI | GAWTC | 1 cut(s) | 785 |
| Tru1I | TTAA | 1 cut(s) | 132 |
| Tru9I | TTAA | 1 cut(s) | 132 |
| TscAI | CASTG | 1 cut(s) | 879 |
| TseI | GCWGC | 3 cut(s) | 164, 370, 796 |
| TspDTI | ATGAA | 1 cut(s) | 210 |
| TspGWI | ACGGA | 1 cut(s) | 847 |
| TspRI | CASTG | 1 cut(s) | 879 |
| XapI | RAATTY | 1 cut(s) | 209 |
| XceI | RCATGY | 1 cut(s) | 367 |
| XmiI | GTMKAC | 1 cut(s) | 527 |
| XmnI | GAANNNNTTC | 1 cut(s) | 390 |
| XspI | CTAG | 2 cut(s) | 174, 771 |
| Zsp2I | ATGCAT | 1 cut(s) | 365 |