MuDR family transposase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 93, 903 |
| AccI | GTMKAC | 1 cut(s) | 524 |
| AclWI | GGATC | 2 cut(s) | 756, 848 |
| AcoI | YGGCCR | 1 cut(s) | 898 |
| AcsI | RAATTY | 1 cut(s) | 209 |
| AdeI | CACNNNGTG | 3 cut(s) | 145, 885, 887 |
| AfaI | GTAC | 1 cut(s) | 550 |
| AgsI | TTSAA | 8 cut(s) | 97, 193, 337, 383, 431, 542, 644, 686 |
| AluBI | AGCT | 5 cut(s) | 17, 164, 333, 466, 788 |
| AluI | AGCT | 5 cut(s) | 17, 164, 333, 466, 788 |
| Alw21I | GWGCWC | 1 cut(s) | 721 |
| Alw26I | GTCTC | 1 cut(s) | 142 |
| AlwI | GGATC | 2 cut(s) | 756, 848 |
| AoxI | GGCC | 1 cut(s) | 898 |
| ApeKI | GCWGC | 2 cut(s) | 164, 793 |
| ApoI | RAATTY | 1 cut(s) | 209 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 387 |
| AspLEI | GCGC | 1 cut(s) | 58 |
| AsuC2I | CCSGG | 2 cut(s) | 746, 819 |
| AsuHPI | GGTGA | 2 cut(s) | 583, 791 |
| BalI | TGGCCA | 1 cut(s) | 900 |
| BbsI | GAAGAC | 3 cut(s) | 390, 704, 829 |
| Bbv12I | GWGCWC | 1 cut(s) | 721 |
| BbvI | GCAGC | 2 cut(s) | 151, 805 |
| BccI | CCATC | 1 cut(s) | 23 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 254, 288 |
| BciVI | GTATCC | 2 cut(s) | 117, 677 |
| BclI | TGATCA | 1 cut(s) | 889 |
| BcnI | CCSGG | 2 cut(s) | 746, 819 |
| BcoDI | GTCTC | 1 cut(s) | 142 |
| BfaI | CTAG | 2 cut(s) | 174, 768 |
| BfmI | CTRYAG | 1 cut(s) | 709 |
| BfuAI | ACCTGC | 2 cut(s) | 93, 903 |
| BfuI | GTATCC | 2 cut(s) | 117, 677 |
| BisI | GCNGC | 2 cut(s) | 165, 794 |
| BlsI | GCNGC | 2 cut(s) | 166, 795 |
| Bme1390I | CCNGG | 2 cut(s) | 746, 819 |
| BmrFI | CCNGG | 2 cut(s) | 746, 819 |
| BmsI | GCATC | 2 cut(s) | 88, 350 |
| BpiI | GAAGAC | 3 cut(s) | 390, 704, 829 |
| Bpu10I | CCTNAGC | 1 cut(s) | 462 |
| BpuEI | CTTGAG | 1 cut(s) | 488 |
| BpuMI | CCSGG | 2 cut(s) | 746, 819 |
| BsaJI | CCNNGG | 2 cut(s) | 10, 827 |
| Bse1I | ACTGG | 4 cut(s) | 520, 698, 740, 869 |
| Bse3DI | GCAATG | 1 cut(s) | 928 |
| BseDI | CCNNGG | 2 cut(s) | 10, 827 |
| BseGI | GGATG | 2 cut(s) | 34, 358 |
| BseMI | GCAATG | 1 cut(s) | 928 |
| BseMII | CTCAG | 1 cut(s) | 386 |
| BseNI | ACTGG | 4 cut(s) | 520, 698, 740, 869 |
| BseRI | GAGGAG | 1 cut(s) | 460 |
| BseXI | GCAGC | 2 cut(s) | 151, 805 |
| BshFI | GGCC | 1 cut(s) | 900 |
| BsiHKAI | GWGCWC | 1 cut(s) | 721 |
| BsiSI | CCGG | 2 cut(s) | 746, 819 |
| BslFI | GGGAC | 1 cut(s) | 728 |
| BsmAI | GTCTC | 1 cut(s) | 142 |
| BsmFI | GGGAC | 1 cut(s) | 728 |
| BsnI | GGCC | 1 cut(s) | 900 |
| Bsp1286I | GDGCHC | 1 cut(s) | 721 |
| Bsp1407I | TGTACA | 1 cut(s) | 548 |
| Bsp143I | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| BspANI | GGCC | 1 cut(s) | 900 |
| BspCNI | CTCAG | 1 cut(s) | 385 |
| BspHI | TCATGA | 1 cut(s) | 721 |
| BspMI | ACCTGC | 2 cut(s) | 93, 903 |
| BspPI | GGATC | 2 cut(s) | 756, 848 |
| BsrDI | GCAATG | 1 cut(s) | 928 |
| BsrGI | TGTACA | 1 cut(s) | 548 |
| BsrI | ACTGG | 4 cut(s) | 520, 698, 740, 869 |
| BssECI | CCNNGG | 2 cut(s) | 10, 827 |
| BssMI | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| Bst4CI | ACNGT | 7 cut(s) | 11, 71, 153, 565, 665, 677, 885 |
| Bst6I | CTCTTC | 1 cut(s) | 852 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 918 |
| BstAUI | TGTACA | 1 cut(s) | 548 |
| BstC8I | GCNNGC | 1 cut(s) | 283 |
| BstDEI | CTNAG | 2 cut(s) | 372, 462 |
| BstDSI | CCRYGG | 2 cut(s) | 10, 827 |
| BstF5I | GGATG | 2 cut(s) | 34, 358 |
| BstHHI | GCGC | 1 cut(s) | 58 |
| BstKTI | GATC | 5 cut(s) | 727, 751, 843, 892, 933 |
| BstMAI | GTCTC | 1 cut(s) | 142 |
| BstMBI | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 170, 376, 918 |
| BstNSI | RCATGY | 1 cut(s) | 367 |
| BstSCI | CCNGG | 2 cut(s) | 744, 817 |
| BstSFI | CTRYAG | 1 cut(s) | 709 |
| BstV1I | GCAGC | 2 cut(s) | 151, 805 |
| BstV2I | GAAGAC | 3 cut(s) | 390, 704, 829 |
| BstX2I | RGATCY | 1 cut(s) | 748 |
| BstYI | RGATCY | 1 cut(s) | 748 |
| BsuI | GTATCC | 2 cut(s) | 117, 677 |
| BsuRI | GGCC | 1 cut(s) | 900 |
| BtgI | CCRYGG | 2 cut(s) | 10, 827 |
| BtsCI | GGATG | 2 cut(s) | 34, 358 |
| BtsIMutI | CAGTG | 2 cut(s) | 561, 876 |
| BveI | ACCTGC | 2 cut(s) | 93, 903 |
| Cac8I | GCNNGC | 1 cut(s) | 283 |
| CciI | TCATGA | 1 cut(s) | 721 |
| CfoI | GCGC | 1 cut(s) | 58 |
| Csp6I | GTAC | 1 cut(s) | 549 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 890, 925 |
| CviAII | CATG | 7 cut(s) | 343, 364, 568, 627, 635, 722, 918 |
| CviJI | RGCY | 9 cut(s) | 17, 164, 333, 466, 519, 760, 788, 796, 900 |
| CviKI_1 | RGCY | 9 cut(s) | 17, 164, 333, 466, 519, 760, 788, 796, 900 |
| CviQI | GTAC | 1 cut(s) | 549 |
| DdeI | CTNAG | 2 cut(s) | 372, 462 |
| DpnI | GATC | 5 cut(s) | 726, 750, 842, 891, 932 |
| DpnII | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| DraIII | CACNNNGTG | 3 cut(s) | 145, 885, 887 |
| EaeI | YGGCCR | 1 cut(s) | 898 |
| Eam1104I | CTCTTC | 1 cut(s) | 852 |
| EarI | CTCTTC | 1 cut(s) | 852 |
| Eco32I | GATATC | 1 cut(s) | 187 |
| EcoRV | GATATC | 1 cut(s) | 187 |
| EcoT22I | ATGCAT | 1 cut(s) | 365 |
| FaeI | CATG | 7 cut(s) | 346, 367, 571, 630, 638, 725, 921 |
| FaqI | GGGAC | 1 cut(s) | 728 |
| FatI | CATG | 7 cut(s) | 342, 363, 567, 626, 634, 721, 917 |
| FauNDI | CATATG | 1 cut(s) | 169 |
| FbaI | TGATCA | 1 cut(s) | 889 |
| FblI | GTMKAC | 1 cut(s) | 524 |
| Fnu4HI | GCNGC | 2 cut(s) | 165, 794 |
| FokI | GGATG | 2 cut(s) | 41, 365 |
| Fsp4HI | GCNGC | 2 cut(s) | 165, 794 |
| FspBI | CTAG | 2 cut(s) | 174, 768 |
| GlaI | GCGC | 1 cut(s) | 57 |
| GluI | GCNGC | 2 cut(s) | 165, 794 |
| HaeIII | GGCC | 1 cut(s) | 900 |
| HapII | CCGG | 2 cut(s) | 746, 819 |
| HhaI | GCGC | 1 cut(s) | 58 |
| Hin1II | CATG | 7 cut(s) | 346, 367, 571, 630, 638, 725, 921 |
| Hin6I | GCGC | 1 cut(s) | 56 |
| HinP1I | GCGC | 1 cut(s) | 56 |
| HindIII | AAGCTT | 1 cut(s) | 464 |
| HinfI | GANTC | 3 cut(s) | 782, 805, 860 |
| HpaII | CCGG | 2 cut(s) | 746, 819 |
| HphI | GGTGA | 2 cut(s) | 583, 791 |
| Hpy166II | GTNNAC | 3 cut(s) | 156, 525, 574 |
| Hpy188I | TCNGA | 1 cut(s) | 935 |
| Hpy188III | TCNNGA | 3 cut(s) | 722, 752, 864 |
| Hpy8I | GTNNAC | 3 cut(s) | 156, 525, 574 |
| HpyAV | CCTTC | 2 cut(s) | 418, 649 |
| HpyCH4III | ACNGT | 7 cut(s) | 11, 71, 153, 565, 665, 677, 885 |
| HpyCH4V | TGCA | 8 cut(s) | 167, 363, 638, 652, 793, 879, 912, 921 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 170, 376, 918 |
| HpyF3I | CTNAG | 2 cut(s) | 372, 462 |
| Hsp92II | CATG | 7 cut(s) | 346, 367, 571, 630, 638, 725, 921 |
| HspAI | GCGC | 1 cut(s) | 56 |
| Ksp22I | TGATCA | 1 cut(s) | 889 |
| Kzo9I | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| LmnI | GCTCC | 1 cut(s) | 14 |
| Lsp1109I | GCAGC | 2 cut(s) | 151, 805 |
| LweI | GCATC | 2 cut(s) | 88, 350 |
| MaeI | CTAG | 2 cut(s) | 174, 768 |
| MaeIII | GTNAC | 1 cut(s) | 677 |
| MalI | GATC | 5 cut(s) | 726, 750, 842, 891, 932 |
| MboI | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| MboII | GAAGA | 9 cut(s) | 181, 218, 395, 426, 698, 709, 719, 834, 869 |
| MfeI | CAATTG | 1 cut(s) | 907 |
| MflI | RGATCY | 1 cut(s) | 748 |
| MhlI | GDGCHC | 1 cut(s) | 721 |
| MlsI | TGGCCA | 1 cut(s) | 900 |
| MluCI | AATT | 5 cut(s) | 91, 209, 316, 505, 907 |
| MluNI | TGGCCA | 1 cut(s) | 900 |
| MlyI | GAGTC | 2 cut(s) | 799, 869 |
| MmeI | TCCRAC | 1 cut(s) | 183 |
| Mox20I | TGGCCA | 1 cut(s) | 900 |
| Mph1103I | ATGCAT | 1 cut(s) | 365 |
| MroXI | GAANNNNTTC | 1 cut(s) | 387 |
| MscI | TGGCCA | 1 cut(s) | 900 |
| MseI | TTAA | 1 cut(s) | 132 |
| MslI | CAYNNNNRTG | 2 cut(s) | 392, 566 |
| Msp20I | TGGCCA | 1 cut(s) | 900 |
| MspI | CCGG | 2 cut(s) | 746, 819 |
| MspR9I | CCNGG | 2 cut(s) | 746, 819 |
| MunI | CAATTG | 1 cut(s) | 907 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 170, 376, 918 |
| NciI | CCSGG | 2 cut(s) | 746, 819 |
| NdeI | CATATG | 1 cut(s) | 169 |
| NdeII | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| NlaIII | CATG | 7 cut(s) | 346, 367, 571, 630, 638, 725, 921 |
| NsiI | ATGCAT | 1 cut(s) | 365 |
| NspI | RCATGY | 1 cut(s) | 367 |
| PagI | TCATGA | 1 cut(s) | 721 |
| PdmI | GAANNNNTTC | 1 cut(s) | 387 |
| PfeI | GAWTC | 1 cut(s) | 782 |
| PfoI | TCCNGGA | 2 cut(s) | 744, 817 |
| PkrI | GCNGC | 2 cut(s) | 166, 795 |
| PleI | GAGTC | 2 cut(s) | 799, 868 |
| PpsI | GAGTC | 2 cut(s) | 799, 868 |
| PsuI | RGATCY | 1 cut(s) | 748 |
| RsaI | GTAC | 1 cut(s) | 550 |
| RsaNI | GTAC | 1 cut(s) | 549 |
| RseI | CAYNNNNRTG | 2 cut(s) | 392, 566 |
| SaqAI | TTAA | 1 cut(s) | 132 |
| SatI | GCNGC | 2 cut(s) | 165, 794 |
| Sau3AI | GATC | 5 cut(s) | 724, 748, 840, 889, 930 |
| SchI | GAGTC | 2 cut(s) | 799, 869 |
| ScrFI | CCNGG | 2 cut(s) | 746, 819 |
| SduI | GDGCHC | 1 cut(s) | 721 |
| SfaNI | GCATC | 2 cut(s) | 88, 350 |
| SfcI | CTRYAG | 1 cut(s) | 709 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 392, 566 |
| SmlI | CTYRAG | 1 cut(s) | 467 |
| SmoI | CTYRAG | 1 cut(s) | 467 |
| Sse9I | AATT | 5 cut(s) | 91, 209, 316, 505, 907 |
| SspMI | CTAG | 2 cut(s) | 174, 768 |
| StyD4I | CCNGG | 2 cut(s) | 744, 817 |
| TaaI | ACNGT | 7 cut(s) | 11, 71, 153, 565, 665, 677, 885 |
| TaqI | TCGA | 2 cut(s) | 482, 808 |
| TasI | AATT | 5 cut(s) | 91, 209, 316, 505, 907 |
| TatI | WGTACW | 1 cut(s) | 548 |
| TfiI | GAWTC | 1 cut(s) | 782 |
| Tru1I | TTAA | 1 cut(s) | 132 |
| Tru9I | TTAA | 1 cut(s) | 132 |
| TscAI | CASTG | 2 cut(s) | 568, 876 |
| TseI | GCWGC | 2 cut(s) | 164, 793 |
| TspDTI | ATGAA | 1 cut(s) | 210 |
| TspGWI | ACGGA | 1 cut(s) | 844 |
| TspRI | CASTG | 2 cut(s) | 568, 876 |
| XapI | RAATTY | 1 cut(s) | 209 |
| XceI | RCATGY | 1 cut(s) | 367 |
| XmiI | GTMKAC | 1 cut(s) | 524 |
| XmnI | GAANNNNTTC | 1 cut(s) | 387 |
| XspI | CTAG | 2 cut(s) | 174, 768 |
| Zsp2I | ATGCAT | 1 cut(s) | 365 |