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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 470 |
| AatII | GACGTC | 1 cut(s) | 474 |
| Acc16I | TGCGCA | 1 cut(s) | 599 |
| Acc36I | ACCTGC | 1 cut(s) | 360 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 174 |
| AccII | CGCG | 1 cut(s) | 283 |
| AciI | CCGC | 5 cut(s) | 71, 223, 253, 281, 487 |
| AclI | AACGTT | 1 cut(s) | 630 |
| AclWI | GGATC | 1 cut(s) | 269 |
| AcoI | YGGCCR | 1 cut(s) | 330 |
| AcyI | GRCGYC | 1 cut(s) | 471 |
| AfaI | GTAC | 1 cut(s) | 520 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 174, 358, 384, 493 |
| AflIII | ACRYGT | 1 cut(s) | 451 |
| AgsI | TTSAA | 2 cut(s) | 542, 563 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 404 |
| AjiI | CACGTC | 2 cut(s) | 454, 483 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 161, 193 |
| AluBI | AGCT | 2 cut(s) | 208, 440 |
| AluI | AGCT | 2 cut(s) | 208, 440 |
| Alw21I | GWGCWC | 2 cut(s) | 49, 624 |
| Alw26I | GTCTC | 1 cut(s) | 169 |
| Alw44I | GTGCAC | 1 cut(s) | 45 |
| AlwI | GGATC | 1 cut(s) | 269 |
| AoxI | GGCC | 2 cut(s) | 156, 330 |
| ApaLI | GTGCAC | 1 cut(s) | 45 |
| ApeKI | GCWGC | 3 cut(s) | 285, 424, 427 |
| AspLEI | GCGC | 2 cut(s) | 285, 600 |
| AspS9I | GGNCC | 1 cut(s) | 157 |
| AsuC2I | CCSGG | 2 cut(s) | 220, 659 |
| AxyI | CCTNAGG | 1 cut(s) | 383 |
| BaeGI | GKGCMC | 1 cut(s) | 49 |
| BalI | TGGCCA | 1 cut(s) | 332 |
| BanII | GRGCYC | 1 cut(s) | 303 |
| BbsI | GAAGAC | 1 cut(s) | 36 |
| Bbv12I | GWGCWC | 2 cut(s) | 49, 624 |
| BbvI | GCAGC | 3 cut(s) | 272, 436, 439 |
| BccI | CCATC | 4 cut(s) | 255, 269, 344, 386 |
| BcnI | CCSGG | 2 cut(s) | 220, 659 |
| BcoDI | GTCTC | 1 cut(s) | 169 |
| BfaI | CTAG | 4 cut(s) | 86, 209, 477, 507 |
| BfuAI | ACCTGC | 1 cut(s) | 360 |
| BisI | GCNGC | 6 cut(s) | 224, 253, 286, 425, 428, 487 |
| BlsI | GCNGC | 6 cut(s) | 225, 254, 287, 426, 429, 488 |
| Bme1390I | CCNGG | 2 cut(s) | 220, 659 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 404 |
| BmgBI | CACGTC | 2 cut(s) | 454, 483 |
| BmgT120I | GGNCC | 1 cut(s) | 157 |
| BmiI | GGNNCC | 2 cut(s) | 217, 300 |
| BmrFI | CCNGG | 2 cut(s) | 220, 659 |
| BpiI | GAAGAC | 1 cut(s) | 36 |
| BpuMI | CCSGG | 2 cut(s) | 220, 659 |
| BsaHI | GRCGYC | 1 cut(s) | 471 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 101, 131, 357, 387 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 174, 358, 384, 493 |
| Bse1I | ACTGG | 1 cut(s) | 159 |
| Bse21I | CCTNAGG | 1 cut(s) | 383 |
| BseGI | GGATG | 1 cut(s) | 378 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 174, 358, 384, 493 |
| BseNI | ACTGG | 1 cut(s) | 159 |
| BseSI | GKGCMC | 1 cut(s) | 49 |
| BseXI | GCAGC | 3 cut(s) | 272, 436, 439 |
| Bsh1236I | CGCG | 1 cut(s) | 283 |
| BshFI | GGCC | 2 cut(s) | 158, 332 |
| BsiHKAI | GWGCWC | 2 cut(s) | 49, 624 |
| BsiSI | CCGG | 3 cut(s) | 220, 490, 658 |
| BslFI | GGGAC | 2 cut(s) | 392, 440 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 174, 358, 384, 493 |
| BsmAI | GTCTC | 1 cut(s) | 169 |
| BsmFI | GGGAC | 2 cut(s) | 392, 440 |
| BsnI | GGCC | 2 cut(s) | 158, 332 |
| Bsp1286I | GDGCHC | 3 cut(s) | 49, 303, 624 |
| Bsp143I | GATC | 1 cut(s) | 274 |
| BspACI | CCGC | 5 cut(s) | 71, 223, 253, 281, 487 |
| BspANI | GGCC | 2 cut(s) | 158, 332 |
| BspFNI | CGCG | 1 cut(s) | 283 |
| BspLI | GGNNCC | 2 cut(s) | 217, 300 |
| BspMI | ACCTGC | 1 cut(s) | 360 |
| BspPI | GGATC | 1 cut(s) | 269 |
| BsrI | ACTGG | 1 cut(s) | 159 |
| BssMI | GATC | 1 cut(s) | 274 |
| BssNI | GRCGYC | 1 cut(s) | 471 |
| Bst4CI | ACNGT | 2 cut(s) | 51, 327 |
| BstACI | GRCGYC | 1 cut(s) | 471 |
| BstDEI | CTNAG | 2 cut(s) | 6, 383 |
| BstF5I | GGATG | 1 cut(s) | 378 |
| BstFNI | CGCG | 1 cut(s) | 283 |
| BstHHI | GCGC | 2 cut(s) | 285, 600 |
| BstKTI | GATC | 1 cut(s) | 277 |
| BstMAI | GTCTC | 1 cut(s) | 169 |
| BstMBI | GATC | 1 cut(s) | 274 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 21 |
| BstSCI | CCNGG | 2 cut(s) | 218, 657 |
| BstSLI | GKGCMC | 1 cut(s) | 49 |
| BstUI | CGCG | 1 cut(s) | 283 |
| BstV1I | GCAGC | 3 cut(s) | 272, 436, 439 |
| BstV2I | GAAGAC | 1 cut(s) | 36 |
| Bsu36I | CCTNAGG | 1 cut(s) | 383 |
| BsuRI | GGCC | 2 cut(s) | 158, 332 |
| BtgZI | GCGATG | 1 cut(s) | 233 |
| BtrI | CACGTC | 2 cut(s) | 454, 483 |
| BtsCI | GGATG | 1 cut(s) | 378 |
| BtsIMutI | CAGTG | 1 cut(s) | 552 |
| BveI | ACCTGC | 1 cut(s) | 360 |
| CfoI | GCGC | 2 cut(s) | 285, 600 |
| Cfr13I | GGNCC | 1 cut(s) | 157 |
| Csp6I | GTAC | 1 cut(s) | 519 |
| CviAII | CATG | 1 cut(s) | 534 |
| CviJI | RGCY | 9 cut(s) | 85, 158, 208, 226, 301, 332, 430, 440, 586 |
| CviKI_1 | RGCY | 9 cut(s) | 85, 158, 208, 226, 301, 332, 430, 440, 586 |
| CviQI | GTAC | 1 cut(s) | 519 |
| DdeI | CTNAG | 2 cut(s) | 6, 383 |
| DpnI | GATC | 1 cut(s) | 276 |
| DpnII | GATC | 1 cut(s) | 274 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 470 |
| DriI | GACNNNNNGTC | 1 cut(s) | 404 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 470 |
| EaeI | YGGCCR | 1 cut(s) | 330 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 404 |
| Eco24I | GRGCYC | 1 cut(s) | 303 |
| Eco81I | CCTNAGG | 1 cut(s) | 383 |
| EcoT38I | GRGCYC | 1 cut(s) | 303 |
| FaeI | CATG | 1 cut(s) | 537 |
| FaiI | YATR | 4 cut(s) | 17, 35, 290, 535 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 575, 607 |
| FaqI | GGGAC | 2 cut(s) | 392, 440 |
| FatI | CATG | 1 cut(s) | 533 |
| FauI | CCCGC | 1 cut(s) | 288 |
| Fnu4HI | GCNGC | 6 cut(s) | 224, 253, 286, 425, 428, 487 |
| FokI | GGATG | 1 cut(s) | 365 |
| FriOI | GRGCYC | 1 cut(s) | 303 |
| Fsp4HI | GCNGC | 6 cut(s) | 224, 253, 286, 425, 428, 487 |
| FspBI | CTAG | 4 cut(s) | 86, 209, 477, 507 |
| FspI | TGCGCA | 1 cut(s) | 599 |
| GlaI | GCGC | 2 cut(s) | 284, 599 |
| GluI | GCNGC | 6 cut(s) | 224, 253, 286, 425, 428, 487 |
| HaeIII | GGCC | 2 cut(s) | 158, 332 |
| HapII | CCGG | 3 cut(s) | 220, 490, 658 |
| HhaI | GCGC | 2 cut(s) | 285, 600 |
| Hin1I | GRCGYC | 1 cut(s) | 471 |
| Hin1II | CATG | 1 cut(s) | 537 |
| Hin6I | GCGC | 2 cut(s) | 283, 598 |
| HinP1I | GCGC | 2 cut(s) | 283, 598 |
| HincII | GTYRAC | 2 cut(s) | 193, 628 |
| HindII | GTYRAC | 2 cut(s) | 193, 628 |
| HpaII | CCGG | 3 cut(s) | 220, 490, 658 |
| Hpy166II | GTNNAC | 3 cut(s) | 47, 193, 628 |
| Hpy188I | TCNGA | 2 cut(s) | 151, 463 |
| Hpy8I | GTNNAC | 3 cut(s) | 47, 193, 628 |
| Hpy99I | CGWCG | 4 cut(s) | 463, 470, 473, 476 |
| HpyCH4III | ACNGT | 2 cut(s) | 51, 327 |
| HpyCH4IV | ACGT | 5 cut(s) | 453, 471, 482, 517, 630 |
| HpyCH4V | TGCA | 3 cut(s) | 47, 288, 396 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 21 |
| HpyF3I | CTNAG | 2 cut(s) | 6, 383 |
| HpySE526I | ACGT | 5 cut(s) | 453, 471, 482, 517, 630 |
| Hsp92I | GRCGYC | 1 cut(s) | 471 |
| Hsp92II | CATG | 1 cut(s) | 537 |
| HspAI | GCGC | 2 cut(s) | 283, 598 |
| Kzo9I | GATC | 1 cut(s) | 274 |
| LmnI | GCTCC | 1 cut(s) | 298 |
| LpnPI | CCDG | 8 cut(s) | 140, 201, 233, 341, 365, 503, 608, 671 |
| Lsp1109I | GCAGC | 3 cut(s) | 272, 436, 439 |
| MaeI | CTAG | 4 cut(s) | 86, 209, 477, 507 |
| MaeII | ACGT | 5 cut(s) | 453, 471, 482, 517, 630 |
| MalI | GATC | 1 cut(s) | 276 |
| MboI | GATC | 1 cut(s) | 274 |
| MboII | GAAGA | 2 cut(s) | 41, 511 |
| MhlI | GDGCHC | 3 cut(s) | 49, 303, 624 |
| MlsI | TGGCCA | 1 cut(s) | 332 |
| MluCI | AATT | 6 cut(s) | 132, 172, 391, 558, 570, 639 |
| MluNI | TGGCCA | 1 cut(s) | 332 |
| MmeI | TCCRAC | 1 cut(s) | 441 |
| MnlI | CCTC | 6 cut(s) | 103, 244, 328, 378, 383, 667 |
| Mox20I | TGGCCA | 1 cut(s) | 332 |
| MscI | TGGCCA | 1 cut(s) | 332 |
| MseI | TTAA | 1 cut(s) | 573 |
| MslI | CAYNNNNRTG | 1 cut(s) | 293 |
| Msp20I | TGGCCA | 1 cut(s) | 332 |
| MspI | CCGG | 3 cut(s) | 220, 490, 658 |
| MspR9I | CCNGG | 2 cut(s) | 220, 659 |
| MvnI | CGCG | 1 cut(s) | 283 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 21 |
| NciI | CCSGG | 2 cut(s) | 220, 659 |
| NdeII | GATC | 1 cut(s) | 274 |
| NlaIII | CATG | 1 cut(s) | 537 |
| NlaIV | GGNNCC | 2 cut(s) | 217, 300 |
| NmeAIII | GCCGAG | 1 cut(s) | 624 |
| NsbI | TGCGCA | 1 cut(s) | 599 |
| PflFI | GACNNNGTC | 2 cut(s) | 188, 470 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 174 |
| PfoI | TCCNGGA | 1 cut(s) | 657 |
| PkrI | GCNGC | 6 cut(s) | 225, 254, 287, 426, 429, 488 |
| Psp1406I | AACGTT | 1 cut(s) | 630 |
| PspN4I | GGNNCC | 2 cut(s) | 217, 300 |
| PspPI | GGNCC | 1 cut(s) | 157 |
| PsyI | GACNNNGTC | 2 cut(s) | 188, 470 |
| RsaI | GTAC | 1 cut(s) | 520 |
| RsaNI | GTAC | 1 cut(s) | 519 |
| RseI | CAYNNNNRTG | 1 cut(s) | 293 |
| SaqAI | TTAA | 1 cut(s) | 573 |
| SatI | GCNGC | 6 cut(s) | 224, 253, 286, 425, 428, 487 |
| Sau3AI | GATC | 1 cut(s) | 274 |
| Sau96I | GGNCC | 1 cut(s) | 157 |
| ScrFI | CCNGG | 2 cut(s) | 220, 659 |
| SduI | GDGCHC | 3 cut(s) | 49, 303, 624 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 293 |
| Sse9I | AATT | 6 cut(s) | 132, 172, 391, 558, 570, 639 |
| SsiI | CCGC | 5 cut(s) | 71, 223, 253, 281, 487 |
| SspI | AATATT | 1 cut(s) | 652 |
| SspMI | CTAG | 4 cut(s) | 86, 209, 477, 507 |
| StyD4I | CCNGG | 2 cut(s) | 218, 657 |
| TaaI | ACNGT | 2 cut(s) | 51, 327 |
| TaiI | ACGT | 5 cut(s) | 456, 474, 485, 520, 633 |
| TaqI | TCGA | 2 cut(s) | 273, 339 |
| TasI | AATT | 6 cut(s) | 132, 172, 391, 558, 570, 639 |
| TauI | GCSGC | 3 cut(s) | 226, 255, 489 |
| Tru1I | TTAA | 1 cut(s) | 573 |
| Tru9I | TTAA | 1 cut(s) | 573 |
| TscAI | CASTG | 1 cut(s) | 559 |
| TseI | GCWGC | 3 cut(s) | 285, 424, 427 |
| TspDTI | ATGAA | 2 cut(s) | 169, 596 |
| TspGWI | ACGGA | 1 cut(s) | 509 |
| TspRI | CASTG | 1 cut(s) | 559 |
| Tth111I | GACNNNGTC | 2 cut(s) | 188, 470 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 174 |
| VneI | GTGCAC | 1 cut(s) | 45 |
| XspI | CTAG | 4 cut(s) | 86, 209, 477, 507 |
| ZraI | GACGTC | 1 cut(s) | 472 |