Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000137

Physical Location & Seq

Genomic Coordinates Reverse (-)

28891497 .. 28893876

Gene Structure 2380 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g40550.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1659 bp

ATGTTGAGTTTCAGTTCTTTCCAAATTCCTACCCATCTCATCATGGAGTTCACTCTTCAATATCTCAGTTCTTCTCCATCTACCATACTTGGCTTTTTATTGTTTATTTTCTCTCTATTGCTATGGATAACAAAGGCTTCCAACACAAACGTAGCCACAAAGAAGGGAGCACCGGAAGCAGCCGGTGCCTGGCCATTAGTTGGTCACCTTCCTCTCTTAGGCGGCTCTCTTCCGCCGCATATAACTTTGGGCAACATGGCTGACAAGTATGGACCGGTCTTCACAATCAAACTCGGGGTGCACCGATCTCTTATTGTGAGCAGTTGGGACATGGCCAAAGAGTGTCTCACCACCAACGACAGAGTCTTTGCTAATCGCCCGAAGCTATTAGTCTCAGAGGTTATGGGCTACAACTACGCCATGTTTGGGTTCAGTCCCTATGGTCCTTACTGGCGCCAGATGCGCAAGGTAGCCACCCTGGAGCTTCTCTCGAACCACCGCTTGGAGTCCCTCAATCACGTTCGTGAATCGGAAGTGAAGACCTGTATTAAGAACATTTACAATTTGTGGAAGCAAAACAAGAACTCAGATCATGTGTTGGTGGACATGAAGAGGTGGTTCGGAGACGTAACGGTGAGCACCATGTTTAGGATGGTTGTAGGGAAGCGGCGCCTACTTGAGACTAGCTCGGAGCGTGAGAAGGAAGAGAACGAAAGGTATCGAAAGGCGACGAGGGAGTTTTTCAGATTGACAGGTGAATTTGTGACGGCGGATGCAGTGCCTTTTCTTAGGTGGTTGGATTTGGGCGGGCATGAGAAGGCAATGAAGAAGACGGCAAAGGAACTAGATGATGTTCTGGAACGATGGTTGGAAGAGCATAAGCTAAAACGAAGTAGTATCAATTCTACTTCTACTTCTCACGAGCCTGACGATTTCATGGATATGATGCTGTCTGTTCTCGATGATAGTATACTGGAGTTTCGTGGGTACACTACTGCTGATACCATCAACAAAGCTACTTGCCTGGCTCTTATATTAGGGGGAACAGACACAACAACAGTAACATTAACATGGGCTCTTTCATTACTAGTGAACAACCCTGCAGTCTTGAAGAAGGCTCAAAATGAATTAGACGCAAAAGTTGGCCGGAATAGACAAGTGAATGAATCAGACGTGAAGAACCTCATGTATCTCCAAGCCGTCATCAAAGAAACCTTGCGCTTATACCCTGCTGTACCACTCCTCGCACCCCATGAATCCACAGAAGATTGCACCGTAGGTAATTACCGTGTACCTGCCAAGACACGTTTAATCGTCAATGTTTGGAAGCTTCACCGGGATCCGAATGTCTGGCAAGATCCCTGCATTTTCCGACCTGAAAGGTTCCTTTCATGCCTTGGAGATTTTGATGTTAGGGGTCAGAATTTTGAGCTCATGCCGTTTGGAAGTGGTAGAAGAATATGTCCAGGAATTTCTTTGGCTCTTCAAGTTATTGAACTCACTCTAGCTCATCTATTGCATGGGTATGAAATTACAGCCCCGTTAGATGAACCGGTCGATATGAGTGAGACTGTTGGGTTAACAAACATGAAGGCCACACCATTACAAGTCCTTCTCAAACCACGCCTTCATGCTGACATTTATGAATATATGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010035 GO:0010038 GO:0010039 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0042221 GO:0044550 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071241 GO:0071248 GO:0071281

KEGG Pathways

Metabolic & Signaling

ko00140 ko00380 ko00830 ko00980 ko00981 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map00981 map01100 map04913 map04917 map04927 map04934 map05204

Pfam Domains

Protein Families

p450

Protein Analysis

553

Amino Acids

62.72

Weight (kDa)

8.05

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	62 - 528	1.2e-93	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000137)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31940 AT4G31950 AT4G31970
fragaria_vesca	FvH4_1g17460 FvH4_2g40550 FvH4_2g40551 FvH4_2g40560 FvH4_2g40570 FvH4_2g40580 FvH4_2g40590 FvH4_7g16520 FvH4_7g16601 FvH4_7g16602 FvH4_7g22620 FvH4_7g22810
malus_domestica	MD00G1037400.v1.1 MD00G1037500.v1.1 MD00G1175000.v1.1 MD00G1175100.v1.1 MD00G1175200.v1.1 MD03G1091600.v1.1 MD04G1043800.v1.1 MD04G1043900.v1.1 MD04G1044100.v1.1 MD04G1044200.v1.1 MD04G1044300.v1.1 MD04G1044400.v1.1 MD08G1234700.v1.1 MD14G1047100.v1.1 MD14G1163500.v1.1 MD15G1028200.v1.1 MD15G1028300.v1.1 MD15G1028400.v1.1 MD15G1028500.v1.1 MD15G1028700.v1.1 MD15G1032900.v1.1 MD15G1033100.v1.1 MD15G1033400.v1.1 MD15G1033800.v1.1 MD15G1033900.v1.1 MD15G1378500.v1.1 MD15G1378800.v1.1
prunus_persica	Prupe.1G386800_v2.0.a1 Prupe.1G386800_v2.0.a1 Prupe.1G386900_v2.0.a1 Prupe.1G387000_v2.0.a1 Prupe.1G387100_v2.0.a1 Prupe.1G387200_v2.0.a1 Prupe.1G387300_v2.0.a1 Prupe.1G387400_v2.0.a1 Prupe.1G387500_v2.0.a1 Prupe.1G387700_v2.0.a1 Prupe.1G537400_v2.0.a1 Prupe.1G537600_v2.0.a1 Prupe.1G537700_v2.0.a1 Prupe.1G538000_v2.0.a1 Prupe.1G538200_v2.0.a1 Prupe.3G064700_v2.0.a1 Prupe.3G066200_v2.0.a1 Prupe.6G212300_v2.0.a1
pyrus_communis	pycom04g03800 pycom04g03820 pycom09g19430 pycom11g16760 pycom14g13610 pycom15g02430 pycom15g02460 pycom15g02470 pycom15g02480 pycom15g02520 pycom15g03050 pycom15g03060 pycom15g03070 pycom15g03090 pycom15g03100 pycom15g03110 pycom15g03120 pycom15g33920
rosa_chinensis	RchiOBHm_Chr1g0359661 RchiOBHm_Chr1g0359671 RchiOBHm_Chr1g0359711 RchiOBHm_Chr1g0359751 RchiOBHm_Chr1g0359791 RchiOBHm_Chr2g0107671 RchiOBHm_Chr2g0107681 RchiOBHm_Chr2g0107691 RchiOBHm_Chr5g0058861 RchiOBHm_Chr5g0058871 RchiOBHm_Chr5g0058891 RchiOBHm_Chr5g0058901 RchiOBHm_Chr5g0058921 RchiOBHm_Chr5g0058931 RchiOBHm_Chr5g0058941 RchiOBHm_Chr5g0058971 RchiOBHm_Chr5g0058991 RchiOBHm_Chr5g0059001 RchiOBHm_Chr5g0060081 RchiOBHm_Chr6g0300111 RchiOBHm_Chr6g0300121 RchiOBHm_Chr6g0300131 RchiOBHm_Chr6g0300141 RchiOBHm_Chr6g0300151 RchiOBHm_Chr6g0300161 RchiOBHm_Chr7g0223401 RchiOBHm_Chr7g0223411 RchiOBHm_Chr7g0233631
rosa_laevigata	RLG00000001247 RLG00000011362 RLG00000011363 RLG00000011364 RLG00000011365 RLG00000011367 RLG00000017599 RLG00000017600 RLG00000027854 RLG00000027856 RLG00000027857 RLG00000035257 RLG00000035258 RLG00000035263 RLG00000035267 RLG00000035268 RLG00000035270 RLG00000035272 RLG00000035273 RLG00000035275 RLG00000035276 RLG00000035343
rosa_multiflora	Rmu_co8382255.1_g000001 Rmu_sc0000441.1_g000120 Rmu_sc0000441.1_g000122 Rmu_sc0000493.1_g000037 Rmu_sc0000493.1_g000038 Rmu_sc0001670.1_g000042 Rmu_sc0001670.1_g000043 Rmu_sc0001670.1_g000044 Rmu_sc0002983.1_g000067 Rmu_sc0002983.1_g000074 Rmu_sc0003366.1_g000019 Rmu_sc0003494.1_g000002 Rmu_sc0003712.1_g000014 Rmu_sc0005703.1_g000003 Rmu_sc0005703.1_g000004 Rmu_sc0006429.1_g000014 Rmu_sc0006429.1_g000018 Rmu_sc0006429.1_g000019 Rmu_sc0008701.1_g000007 Rmu_sc0009050.1_g000002 Rmu_sc0009050.1_g000008 Rmu_sc0014587.1_g000001 Rmu_sc0034954.1_g000001 Rmu_ssc0000371.1_g000008 Rmu_ssc0000371.1_g000009 Rmu_ssc0000371.1_g000012 Rmu_ssc0000371.1_g000025 Rmu_ssc0000371.1_g000028
rosa_roxburghii	Rroxscaffold_1G00021390 Rroxscaffold_1G00021450 Rroxscaffold_1G00021460 Rroxscaffold_1G00021470 Rroxscaffold_1G00021480 Rroxscaffold_1G00021500 Rroxscaffold_2G00136030 Rroxscaffold_2G00136040 Rroxscaffold_2G00136070 Rroxscaffold_3G00227100 Rroxscaffold_4G00296830 Rroxscaffold_4G00296860 Rroxscaffold_4G00296870 Rroxscaffold_7G00168050 Rroxscaffold_7G00168060 Rroxscaffold_7G00168080 Rroxscaffold_7G00168090
rosa_rugosa	Rorug01G0272400 Rorug01G0272500 Rorug01G0272600 Rorug01G0272700 Rorug02G0146800.1 Rorug03G0204200 Rorug03G0204300 Rorug05G0319800 Rorug05G0319900 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug07G0281800
rosa_samantha	Rh5DG412300 Rh5DG412400 Rh6CG421000 Rh7DG427300
rosa_wichuraiana	Rw1G025420 Rw1G025430 Rw1G025440 Rw2G015520 Rw5G036240 Rw5G036250 Rw5G036260 Rw5G036270 Rw5G036950 Rw6G035500 Rw6G035510 Rw6G035520 Rw7G036140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	464
Acc36I	ACCTGC	1 cut(s)	1303
AccB1I	GGYRCC	3 cut(s)	185, 453, 669
AccB7I	CCANNNNNTGG	2 cut(s)	200, 502
AccI	GTMKAC	1 cut(s)	970
AciI	CCGC	7 cut(s)	222, 233, 236, 499, 667, 770, 807
AclWI	GGATC	3 cut(s)	1334, 1347, 1352
AcoI	YGGCCR	3 cut(s)	191, 333, 1144
AcsI	RAATTY	4 cut(s)	24, 758, 1423, 1470
AcyI	GRCGYC	2 cut(s)	454, 670
AfaI	GTAC	3 cut(s)	989, 1236, 1293
AfiI	CCNNNNNNNGG	6 cut(s)	189, 200, 218, 426, 502, 648
AflIII	ACRYGT	1 cut(s)	1304
AgeI	ACCGGT	2 cut(s)	274, 1552
AgsI	TTSAA	4 cut(s)	59, 1111, 1487, 1496
AhlI	ACTAGT	1 cut(s)	1087
AjiI	CACGTC	1 cut(s)	1174
AjnI	CCWGG	4 cut(s)	188, 477, 1023, 1465
AjuI	GAANNNNNNNTTGG	2 cut(s)	485, 517
AleI	CACNNNNGTG	1 cut(s)	522
AluBI	AGCT	8 cut(s)	385, 484, 687, 883, 1016, 1330, 1432, 1508
AluI	AGCT	8 cut(s)	385, 484, 687, 883, 1016, 1330, 1432, 1508
Alw21I	GWGCWC	4 cut(s)	172, 303, 641, 1434
Alw26I	GTCTC	5 cut(s)	350, 397, 618, 674, 1562
Alw44I	GTGCAC	1 cut(s)	299
AlwI	GGATC	3 cut(s)	1334, 1347, 1352
Ama87I	CYCGRG	1 cut(s)	293
AoxI	GGCC	4 cut(s)	191, 333, 1144, 1593
ApaLI	GTGCAC	1 cut(s)	299
ApeKI	GCWGC	1 cut(s)	179
ApoI	RAATTY	4 cut(s)	24, 758, 1423, 1470
ArsI	GACNNNNNNTTYG	2 cut(s)	350, 382
AsiGI	ACCGGT	2 cut(s)	274, 1552
AspLEI	GCGC	4 cut(s)	456, 465, 672, 1221
AspS9I	GGNCC	2 cut(s)	272, 443
AsuC2I	CCSGG	1 cut(s)	1337
AsuHPI	GGTGA	5 cut(s)	197, 340, 646, 767, 1325
AvaI	CYCGRG	1 cut(s)	293
AvaII	GGWCC	2 cut(s)	272, 443
BaeGI	GKGCMC	1 cut(s)	303
BalI	TGGCCA	2 cut(s)	193, 335
BamHI	GGATCC	1 cut(s)	1339
BanI	GGYRCC	3 cut(s)	185, 453, 669
BanII	GRGCYC	2 cut(s)	1078, 1434
BauI	CACGAG	1 cut(s)	920
BbsI	GAAGAC	3 cut(s)	271, 545, 836
Bbv12I	GWGCWC	4 cut(s)	172, 303, 641, 1434
BbvI	GCAGC	1 cut(s)	191
BccI	CCATC	5 cut(s)	42, 85, 646, 858, 1013
BceAI	ACGGC	4 cut(s)	783, 849, 1184, 1423
BciT130I	CCWGG	4 cut(s)	190, 479, 1025, 1467
BcnI	CCSGG	1 cut(s)	1337
BcoDI	GTCTC	5 cut(s)	350, 397, 618, 674, 1562
BcuI	ACTAGT	1 cut(s)	1087
BfaI	CTAG	4 cut(s)	684, 845, 1088, 1505
BfmI	CTRYAG	1 cut(s)	1101
BfoI	RGCGCY	2 cut(s)	457, 673
BfuAI	ACCTGC	1 cut(s)	1303
BisI	GCNGC	4 cut(s)	180, 223, 236, 668
BlsI	GCNGC	4 cut(s)	181, 224, 237, 669
Bme1390I	CCNGG	5 cut(s)	190, 479, 1025, 1337, 1467
Bme18I	GGWCC	2 cut(s)	272, 443
BmeT110I	CYCGRG	1 cut(s)	293
BmgBI	CACGTC	1 cut(s)	1174
BmgT120I	GGNCC	2 cut(s)	272, 443
BmiI	GGNNCC	5 cut(s)	187, 455, 671, 1341, 1385
BmrFI	CCNGG	5 cut(s)	190, 479, 1025, 1337, 1467
BmsI	GCATC	3 cut(s)	450, 763, 936
BpiI	GAAGAC	3 cut(s)	271, 545, 836
BplI	GAGNNNNNCTC	4 cut(s)	473, 505, 671, 703
BpmI	CTGGAG	2 cut(s)	500, 995
BpuEI	CTTGAG	1 cut(s)	698
BpuMI	CCSGG	1 cut(s)	1337
BsaHI	GRCGYC	2 cut(s)	454, 670
BsaJI	CCNNGG	2 cut(s)	477, 1396
BsaWI	WCCGGW	3 cut(s)	172, 274, 1552
Bsc4I	CCNNNNNNNGG	6 cut(s)	189, 200, 218, 426, 502, 648
Bse118I	RCCGGY	3 cut(s)	182, 274, 1552
Bse1I	ACTGG	2 cut(s)	455, 978
Bse3DI	GCAATG	1 cut(s)	828
BseBI	CCWGG	4 cut(s)	190, 479, 1025, 1467
BseDI	CCNNGG	2 cut(s)	477, 1396
BseGI	GGATG	2 cut(s)	657, 778
BseLI	CCNNNNNNNGG	6 cut(s)	189, 200, 218, 426, 502, 648
BseMI	GCAATG	1 cut(s)	828
BseMII	CTCAG	3 cut(s)	79, 408, 600
BseNI	ACTGG	2 cut(s)	455, 978
BseRI	GAGGAG	1 cut(s)	1232
BseSI	GKGCMC	1 cut(s)	303
BseXI	GCAGC	1 cut(s)	191
Bsh1285I	CGRYCG	1 cut(s)	1557
BshFI	GGCC	4 cut(s)	193, 335, 1146, 1595
BshNI	GGYRCC	3 cut(s)	185, 453, 669
BshTI	ACCGGT	2 cut(s)	274, 1552
BsiEI	CGRYCG	1 cut(s)	1557
BsiHKAI	GWGCWC	4 cut(s)	172, 303, 641, 1434
BsiHKCI	CYCGRG	1 cut(s)	293
BsiSI	CCGG	6 cut(s)	173, 183, 275, 1147, 1336, 1553
BslFI	GGGAC	3 cut(s)	341, 420, 493
BslI	CCNNNNNNNGG	6 cut(s)	189, 200, 218, 426, 502, 648
BsmAI	GTCTC	5 cut(s)	350, 397, 618, 674, 1562
BsmBI	CGTCTC	1 cut(s)	618
BsmFI	GGGAC	3 cut(s)	341, 420, 493
BsnI	GGCC	4 cut(s)	193, 335, 1146, 1595
BsoBI	CYCGRG	1 cut(s)	293
Bsp1286I	GDGCHC	5 cut(s)	172, 303, 641, 1078, 1434
Bsp143I	GATC	4 cut(s)	305, 589, 1339, 1357
BspACI	CCGC	7 cut(s)	222, 233, 236, 499, 667, 770, 807
BspANI	GGCC	4 cut(s)	193, 335, 1146, 1595
BspCNI	CTCAG	3 cut(s)	78, 407, 599
BspLI	GGNNCC	5 cut(s)	187, 455, 671, 1341, 1385
BspMAI	CTGCAG	1 cut(s)	1105
BspMI	ACCTGC	1 cut(s)	1303
BspPI	GGATC	3 cut(s)	1334, 1347, 1352
BspQI	GCTCTTC	2 cut(s)	867, 1488
BspT107I	GGYRCC	3 cut(s)	185, 453, 669
BsrDI	GCAATG	1 cut(s)	828
BsrFI	RCCGGY	3 cut(s)	182, 274, 1552
BsrI	ACTGG	2 cut(s)	455, 978
BssAI	RCCGGY	3 cut(s)	182, 274, 1552
BssECI	CCNNGG	2 cut(s)	477, 1396
BssMI	GATC	4 cut(s)	305, 589, 1339, 1357
BssNAI	GTATAC	1 cut(s)	971
BssNI	GRCGYC	2 cut(s)	454, 670
BssSI	CACGAG	1 cut(s)	920
BssT1I	CCWWGG	1 cut(s)	1396
Bst1107I	GTATAC	1 cut(s)	971
Bst2BI	CACGAG	1 cut(s)	920
Bst2UI	CCWGG	4 cut(s)	190, 479, 1025, 1467
Bst4CI	ACNGT	5 cut(s)	634, 1060, 1276, 1289, 1573
Bst6I	CTCTTC	6 cut(s)	60, 234, 605, 699, 867, 1488
BstACI	GRCGYC	2 cut(s)	454, 670
BstAPI	GCANNNNNTGC	1 cut(s)	185
BstC8I	GCNNGC	1 cut(s)	809
BstDEI	CTNAG	5 cut(s)	65, 217, 394, 586, 788
BstEII	GGTNACC	1 cut(s)	203
BstENI	CCTNNNNNAGG	1 cut(s)	216
BstF5I	GGATG	2 cut(s)	657, 778
BstH2I	RGCGCY	2 cut(s)	457, 673
BstHHI	GCGC	4 cut(s)	456, 465, 672, 1221
BstKTI	GATC	4 cut(s)	308, 592, 1342, 1360
BstMAI	GTCTC	5 cut(s)	350, 397, 618, 674, 1562
BstMBI	GATC	4 cut(s)	305, 589, 1339, 1357
BstMCI	CGRYCG	1 cut(s)	1557
BstMWI	GCNNNNNNNGC	4 cut(s)	176, 185, 460, 462
BstNI	CCWGG	4 cut(s)	190, 479, 1025, 1467
BstPI	GGTNACC	1 cut(s)	203
BstSCI	CCNGG	5 cut(s)	188, 477, 1023, 1335, 1465
BstSFI	CTRYAG	1 cut(s)	1101
BstSLI	GKGCMC	1 cut(s)	303
BstV1I	GCAGC	1 cut(s)	191
BstV2I	GAAGAC	3 cut(s)	271, 545, 836
BstX2I	RGATCY	2 cut(s)	1339, 1357
BstYI	RGATCY	2 cut(s)	1339, 1357
BstZ17I	GTATAC	1 cut(s)	971
BsuRI	GGCC	4 cut(s)	193, 335, 1146, 1595
BtrI	CACGTC	1 cut(s)	1174
BtsCI	GGATG	2 cut(s)	657, 778
BtsI	GCAGTG	1 cut(s)	783
BtsIMutI	CAGTG	1 cut(s)	783
BveI	ACCTGC	1 cut(s)	1303
Cac8I	GCNNGC	1 cut(s)	809
CfoI	GCGC	4 cut(s)	456, 465, 672, 1221
Cfr10I	RCCGGY	3 cut(s)	182, 274, 1552
Cfr13I	GGNCC	2 cut(s)	272, 443
CseI	GACGC	1 cut(s)	1142
Csp6I	GTAC	3 cut(s)	988, 1235, 1292
CspAI	ACCGGT	2 cut(s)	274, 1552
CviQI	GTAC	3 cut(s)	988, 1235, 1292
DdeI	CTNAG	5 cut(s)	65, 217, 394, 586, 788
DinI	GGCGCC	2 cut(s)	455, 671
DpnI	GATC	4 cut(s)	307, 591, 1341, 1359
DpnII	GATC	4 cut(s)	305, 589, 1339, 1357
EaeI	YGGCCR	3 cut(s)	191, 333, 1144
Eam1104I	CTCTTC	6 cut(s)	60, 234, 605, 699, 867, 1488
EarI	CTCTTC	6 cut(s)	60, 234, 605, 699, 867, 1488
EciI	GGCGGA	2 cut(s)	222, 785
Ecl136II	GAGCTC	1 cut(s)	1432
Eco130I	CCWWGG	1 cut(s)	1396
Eco24I	GRGCYC	2 cut(s)	1078, 1434
Eco47I	GGWCC	2 cut(s)	272, 443
Eco53kI	GAGCTC	1 cut(s)	1432
Eco88I	CYCGRG	1 cut(s)	293
Eco91I	GGTNACC	1 cut(s)	203
EcoICRI	GAGCTC	1 cut(s)	1432
EcoNI	CCTNNNNNAGG	1 cut(s)	216
EcoO65I	GGTNACC	1 cut(s)	203
EcoRII	CCWGG	4 cut(s)	188, 477, 1023, 1465
EcoT14I	CCWWGG	1 cut(s)	1396
EcoT38I	GRGCYC	2 cut(s)	1078, 1434
EgeI	GGCGCC	2 cut(s)	455, 671
EheI	GGCGCC	2 cut(s)	455, 671
ErhI	CCWWGG	1 cut(s)	1396
Esp3I	CGTCTC	1 cut(s)	618
FaqI	GGGAC	3 cut(s)	341, 420, 493
FauI	CCCGC	1 cut(s)	800
FblI	GTMKAC	1 cut(s)	970
Fnu4HI	GCNGC	4 cut(s)	180, 223, 236, 668
FokI	GGATG	2 cut(s)	664, 785
FriOI	GRGCYC	2 cut(s)	1078, 1434
Fsp4HI	GCNGC	4 cut(s)	180, 223, 236, 668
FspBI	CTAG	4 cut(s)	684, 845, 1088, 1505
FspI	TGCGCA	1 cut(s)	464
GlaI	GCGC	4 cut(s)	455, 464, 671, 1220
GluI	GCNGC	4 cut(s)	180, 223, 236, 668
GsuI	CTGGAG	2 cut(s)	500, 995
HaeII	RGCGCY	2 cut(s)	457, 673
HaeIII	GGCC	4 cut(s)	193, 335, 1146, 1595
HapII	CCGG	6 cut(s)	173, 183, 275, 1147, 1336, 1553
HgaI	GACGC	1 cut(s)	1142
HhaI	GCGC	4 cut(s)	456, 465, 672, 1221
Hin1I	GRCGYC	2 cut(s)	454, 670
Hin6I	GCGC	4 cut(s)	454, 463, 670, 1219
HinP1I	GCGC	4 cut(s)	454, 463, 670, 1219
HincII	GTYRAC	1 cut(s)	1581
HindII	GTYRAC	1 cut(s)	1581
HindIII	AAGCTT	1 cut(s)	1328
HinfI	GANTC	5 cut(s)	363, 506, 527, 1166, 1256
HpaI	GTTAAC	1 cut(s)	1581
HpaII	CCGG	6 cut(s)	173, 183, 275, 1147, 1336, 1553
HphI	GGTGA	5 cut(s)	197, 340, 646, 767, 1325
Hpy166II	GTNNAC	8 cut(s)	51, 301, 604, 971, 990, 1093, 1292, 1581
Hpy188III	TCNNGA	6 cut(s)	490, 524, 857, 920, 959, 1108
Hpy8I	GTNNAC	8 cut(s)	51, 301, 604, 971, 990, 1093, 1292, 1581
Hpy99I	CGWCG	1 cut(s)	733
HpyAV	CCTTC	8 cut(s)	157, 218, 694, 811, 1108, 1585, 1622, 1637
HpyCH4III	ACNGT	5 cut(s)	634, 1060, 1276, 1289, 1573
HpyCH4IV	ACGT	5 cut(s)	150, 519, 627, 1173, 1306
HpyCH4V	TGCA	6 cut(s)	301, 776, 1103, 1272, 1365, 1519
HpyF10VI	GCNNNNNNNGC	4 cut(s)	176, 185, 460, 462
HpyF3I	CTNAG	5 cut(s)	65, 217, 394, 586, 788
HpySE526I	ACGT	5 cut(s)	150, 519, 627, 1173, 1306
Hsp92I	GRCGYC	2 cut(s)	454, 670
HspAI	GCGC	4 cut(s)	454, 463, 670, 1219
KasI	GGCGCC	2 cut(s)	453, 669
KspAI	GTTAAC	1 cut(s)	1581
Kzo9I	GATC	4 cut(s)	305, 589, 1339, 1357
LguI	GCTCTTC	2 cut(s)	867, 1488
LmnI	GCTCC	3 cut(s)	167, 481, 691
Lsp1109I	GCAGC	1 cut(s)	191
LweI	GCATC	3 cut(s)	450, 763, 936
MaeI	CTAG	4 cut(s)	684, 845, 1088, 1505
MaeII	ACGT	5 cut(s)	150, 519, 627, 1173, 1306
MaeIII	GTNAC	4 cut(s)	203, 628, 763, 1060
MalI	GATC	4 cut(s)	307, 591, 1341, 1359
MboI	GATC	4 cut(s)	305, 589, 1339, 1357
MflI	RGATCY	2 cut(s)	1339, 1357
MhlI	GDGCHC	5 cut(s)	172, 303, 641, 1078, 1434
MlsI	TGGCCA	2 cut(s)	193, 335
MluCI	AATT	9 cut(s)	24, 562, 758, 901, 1127, 1282, 1423, 1470, 1530
MluNI	TGGCCA	2 cut(s)	193, 335
Mly113I	GGCGCC	2 cut(s)	454, 670
MlyI	GAGTC	2 cut(s)	372, 515
MmeI	TCCRAC	4 cut(s)	165, 777, 849, 1396
MnlI	CCTC	7 cut(s)	222, 391, 521, 606, 726, 1193, 1253
Mox20I	TGGCCA	2 cut(s)	193, 335
MscI	TGGCCA	2 cut(s)	193, 335
MseI	TTAA	4 cut(s)	549, 1067, 1310, 1580
MslI	CAYNNNNRTG	4 cut(s)	522, 941, 1069, 1524
Msp20I	TGGCCA	2 cut(s)	193, 335
MspI	CCGG	6 cut(s)	173, 183, 275, 1147, 1336, 1553
MspR9I	CCNGG	5 cut(s)	190, 479, 1025, 1337, 1467
MvaI	CCWGG	4 cut(s)	190, 479, 1025, 1467
MwoI	GCNNNNNNNGC	4 cut(s)	176, 185, 460, 462
NarI	GGCGCC	2 cut(s)	454, 670
NciI	CCSGG	1 cut(s)	1337
NdeII	GATC	4 cut(s)	305, 589, 1339, 1357
NlaIV	GGNNCC	5 cut(s)	187, 455, 671, 1341, 1385
NmuCI	GTSAC	2 cut(s)	203, 763
NsbI	TGCGCA	1 cut(s)	464
OliI	CACNNNNGTG	1 cut(s)	522
PciSI	GCTCTTC	2 cut(s)	867, 1488
PcsI	WCGNNNNNNNCGW	1 cut(s)	927
PfeI	GAWTC	3 cut(s)	527, 1166, 1256
PflFI	GACNNNGTC	1 cut(s)	362
PflMI	CCANNNNNTGG	2 cut(s)	200, 502
PfoI	TCCNGGA	1 cut(s)	1465
PinAI	ACCGGT	2 cut(s)	274, 1552
PkrI	GCNGC	4 cut(s)	181, 224, 237, 669
PleI	GAGTC	2 cut(s)	371, 514
PluTI	GGCGCC	2 cut(s)	457, 673
PpsI	GAGTC	2 cut(s)	371, 514
Psp124BI	GAGCTC	1 cut(s)	1434
Psp6I	CCWGG	4 cut(s)	188, 477, 1023, 1465
PspEI	GGTNACC	1 cut(s)	203
PspGI	CCWGG	4 cut(s)	188, 477, 1023, 1465
PspN4I	GGNNCC	5 cut(s)	187, 455, 671, 1341, 1385
PspPI	GGNCC	2 cut(s)	272, 443
PstI	CTGCAG	1 cut(s)	1105
PsuI	RGATCY	2 cut(s)	1339, 1357
PsyI	GACNNNGTC	1 cut(s)	362
RsaI	GTAC	3 cut(s)	989, 1236, 1293
RsaNI	GTAC	3 cut(s)	988, 1235, 1292
RseI	CAYNNNNRTG	4 cut(s)	522, 941, 1069, 1524
SacI	GAGCTC	1 cut(s)	1434
SapI	GCTCTTC	2 cut(s)	867, 1488
SaqAI	TTAA	4 cut(s)	549, 1067, 1310, 1580
SatI	GCNGC	4 cut(s)	180, 223, 236, 668
Sau3AI	GATC	4 cut(s)	305, 589, 1339, 1357
Sau96I	GGNCC	2 cut(s)	272, 443
SchI	GAGTC	2 cut(s)	372, 515
ScrFI	CCNGG	5 cut(s)	190, 479, 1025, 1337, 1467
SduI	GDGCHC	5 cut(s)	172, 303, 641, 1078, 1434
SfaNI	GCATC	3 cut(s)	450, 763, 936
SfcI	CTRYAG	1 cut(s)	1101
SfoI	GGCGCC	2 cut(s)	455, 671
SinI	GGWCC	2 cut(s)	272, 443
SmiMI	CAYNNNNRTG	4 cut(s)	522, 941, 1069, 1524
SmlI	CTYRAG	1 cut(s)	677
SmoI	CTYRAG	1 cut(s)	677
SpeI	ACTAGT	1 cut(s)	1087
Sse9I	AATT	9 cut(s)	24, 562, 758, 901, 1127, 1282, 1423, 1470, 1530
SsiI	CCGC	7 cut(s)	222, 233, 236, 499, 667, 770, 807
SspDI	GGCGCC	2 cut(s)	453, 669
SspMI	CTAG	4 cut(s)	684, 845, 1088, 1505
SstI	GAGCTC	1 cut(s)	1434
StyD4I	CCNGG	5 cut(s)	188, 477, 1023, 1335, 1465
StyI	CCWWGG	1 cut(s)	1396
TaaI	ACNGT	5 cut(s)	634, 1060, 1276, 1289, 1573
TaiI	ACGT	5 cut(s)	153, 522, 630, 1176, 1309
TaqI	TCGA	4 cut(s)	491, 721, 960, 1557
TasI	AATT	9 cut(s)	24, 562, 758, 901, 1127, 1282, 1423, 1470, 1530
TauI	GCSGC	3 cut(s)	225, 238, 670
TfiI	GAWTC	3 cut(s)	527, 1166, 1256
Tru1I	TTAA	4 cut(s)	549, 1067, 1310, 1580
Tru9I	TTAA	4 cut(s)	549, 1067, 1310, 1580
TscAI	CASTG	1 cut(s)	783
TseFI	GTSAC	2 cut(s)	203, 763
TseI	GCWGC	1 cut(s)	179
Tsp45I	GTSAC	2 cut(s)	203, 763
TspRI	CASTG	1 cut(s)	783
Tth111I	GACNNNGTC	1 cut(s)	362
Van91I	CCANNNNNTGG	2 cut(s)	200, 502
VneI	GTGCAC	1 cut(s)	299
VpaK11BI	GGWCC	2 cut(s)	272, 443
XagI	CCTNNNNNAGG	1 cut(s)	216
XapI	RAATTY	4 cut(s)	24, 758, 1423, 1470
XcmI	CCANNNNNNNNNTGG	1 cut(s)	649
XmiI	GTMKAC	1 cut(s)	970
XspI	CTAG	4 cut(s)	684, 845, 1088, 1505

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.