Rw1G025420

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000137
Physical Location & Seq
Forward (+)
52046062 .. 52049692
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025420.1

Sequence Viewer

Length: 1611 bp
ATGGAGTTTCTGCTTCCATTTTCCATTTGTGATCCAACTACCATCTTAGCATTTTTACTATTTCTTTTCTCTTCCGTATGGATATCAAAATACTTGCAGAAAAACACAGCAAAGAACAAAGGACCCCCAGAAGTGGCTGGTGCATGGCCAATACTTGGCCACCTTCCTCTCTTAAGAGGGCCTCAACCGCCCCACATAATCCTAGCGAACATGGCTGACAAGTACGGACCGCTCTTCACTATCAAGCTTGGTGTGCACCGAGCTCTTATTGTAAGCAGTTGGGATGTGGCTAAAGAGTGTTTCACCACTAATGATAAAGCTTTTGTCAACCGCCCGAAAGCTTTAAGCCCGGAGATTTTGGGGTACGACTATGCCATGTTCGGTTTTAGCCCTTACGGACCTTACTGGCGCCAGGTGCGAAAGATAGCCACGCTGCACATCCTCTCCAACCACAAGTTGGAGTTGCTCAAGCACGTCCGGGAAACGGAGGTGAAGGTGTCTACGAAACATATATTTGATATGTGGGAGCGAAACAAAGGTGTCTCAAATAAGGTGCTGGTGGACATGAAGAGGTGGTTTACAGACTTAACTTTAAATGTCATGTTCAGGATGGTTGTTGGAAAGCGACTTCTTGGGGCTACCACGGCGCAAGAGAAGGAAAAGAACGAAAGCTTTCGAAAGGTGTTGAGGGATTTTTTTATATTAGCTGGTCAATTTGTAATCTCAGATGCAGTTCCATATCTTAGGTGGTTGGATTTGGGAGGTTATGAGAAGGCCATGAAGAAGACGGCTAAAGAACTCGATGGTGCGGCCCAAAAATGGCTAGAAGAGCATAAGCACAGGAGAAGTACTAATTCTGTAGAAGAGCCTGACTTCATGGATATGATGCTTACTGTCCTTGATGATGATAGCACAGAAGAGTTTGCTGGTTCAATTACTGCTGATACAATTAACAAAGCTACATGCCTGGCGGTTCTTCTAGGAGGGACAGAAACCTCATCAGTGACATTAATCTGGGCTCTTTCTTTATTGCTCAACAATCCTAAAGTCCTGAAGAAGGCTCAACTTGAGTTAGACAACCATGTTGGTAGGGAAAGGCAAGTGAATGAATCAGATGTGAAGAATCTAGTCTACCTCAAAGCCATTATCAAGGAAACCTTGCGGCTATACCCTGCTGCACCTCTCTCAGTACCACATGAATCCAGCGAAGACTGCAAAATAGGTAACTATCATGTAAGTTCAGGCACGCGTCTTCTTATCAACCTTTCAAAGCTCCAGCGCGATCCAAATGTCTGGCTCGATCCTTGTCTATTCCAACCAGAAAGGTTTCTTACAACTCACAAGAATGTTGATGTTAGGGGCCAGAGTTTTGAATTTATACCATTTGGAAGTGGTAGACGAATATGTCCAGGAATCTCTCTCGCACTTCACGTTACTCAACTTGCACTGGCTCATTTGCTGCATGGGTTTGAAATTGCGACTCCATCTGATGAACCAGTTGACATGGGTGAGAGTTCAGGAATAACAAACATGAAGACCAGCCCTCTGGAGCTACGAATTGGGGAAAAACATCAGCATGAATCGGATCACTTCAACTCATGGCCACATTGA

Protein Analysis

536

Amino Acids

60.69

Weight (kDa)

8.34

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 45 - 509 6.5e-92 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31940 AT4G31950 AT4G31970
fragaria_vesca FvH4_1g17460 FvH4_2g40550 FvH4_2g40551 FvH4_2g40560 FvH4_2g40570 FvH4_2g40580 FvH4_2g40590 FvH4_7g16520 FvH4_7g16601 FvH4_7g16602 FvH4_7g22620 FvH4_7g22810
malus_domestica MD00G1037400.v1.1 MD00G1037500.v1.1 MD00G1175000.v1.1 MD00G1175100.v1.1 MD00G1175200.v1.1 MD03G1091600.v1.1 MD04G1043800.v1.1 MD04G1043900.v1.1 MD04G1044100.v1.1 MD04G1044200.v1.1 MD04G1044300.v1.1 MD04G1044400.v1.1 MD08G1234700.v1.1 MD14G1047100.v1.1 MD14G1163500.v1.1 MD15G1028200.v1.1 MD15G1028300.v1.1 MD15G1028400.v1.1 MD15G1028500.v1.1 MD15G1028700.v1.1 MD15G1032900.v1.1 MD15G1033100.v1.1 MD15G1033400.v1.1 MD15G1033800.v1.1 MD15G1033900.v1.1 MD15G1378500.v1.1 MD15G1378800.v1.1
prunus_persica Prupe.1G386800_v2.0.a1 Prupe.1G386800_v2.0.a1 Prupe.1G386900_v2.0.a1 Prupe.1G387000_v2.0.a1 Prupe.1G387100_v2.0.a1 Prupe.1G387200_v2.0.a1 Prupe.1G387300_v2.0.a1 Prupe.1G387400_v2.0.a1 Prupe.1G387500_v2.0.a1 Prupe.1G387700_v2.0.a1 Prupe.1G537400_v2.0.a1 Prupe.1G537600_v2.0.a1 Prupe.1G537700_v2.0.a1 Prupe.1G538000_v2.0.a1 Prupe.1G538200_v2.0.a1 Prupe.3G064700_v2.0.a1 Prupe.3G066200_v2.0.a1 Prupe.6G212300_v2.0.a1
pyrus_communis pycom04g03800 pycom04g03820 pycom09g19430 pycom11g16760 pycom14g13610 pycom15g02430 pycom15g02460 pycom15g02470 pycom15g02480 pycom15g02520 pycom15g03050 pycom15g03060 pycom15g03070 pycom15g03090 pycom15g03100 pycom15g03110 pycom15g03120 pycom15g33920
rosa_chinensis RchiOBHm_Chr1g0359661 RchiOBHm_Chr1g0359671 RchiOBHm_Chr1g0359711 RchiOBHm_Chr1g0359751 RchiOBHm_Chr1g0359791 RchiOBHm_Chr2g0107671 RchiOBHm_Chr2g0107681 RchiOBHm_Chr2g0107691 RchiOBHm_Chr5g0058861 RchiOBHm_Chr5g0058871 RchiOBHm_Chr5g0058891 RchiOBHm_Chr5g0058901 RchiOBHm_Chr5g0058921 RchiOBHm_Chr5g0058931 RchiOBHm_Chr5g0058941 RchiOBHm_Chr5g0058971 RchiOBHm_Chr5g0058991 RchiOBHm_Chr5g0059001 RchiOBHm_Chr5g0060081 RchiOBHm_Chr6g0300111 RchiOBHm_Chr6g0300121 RchiOBHm_Chr6g0300131 RchiOBHm_Chr6g0300141 RchiOBHm_Chr6g0300151 RchiOBHm_Chr6g0300161 RchiOBHm_Chr7g0223401 RchiOBHm_Chr7g0223411 RchiOBHm_Chr7g0233631
rosa_laevigata RLG00000001247 RLG00000011362 RLG00000011363 RLG00000011364 RLG00000011365 RLG00000011367 RLG00000017599 RLG00000017600 RLG00000027854 RLG00000027856 RLG00000027857 RLG00000035257 RLG00000035258 RLG00000035263 RLG00000035267 RLG00000035268 RLG00000035270 RLG00000035272 RLG00000035273 RLG00000035275 RLG00000035276 RLG00000035343
rosa_multiflora Rmu_co8382255.1_g000001 Rmu_sc0000441.1_g000120 Rmu_sc0000441.1_g000122 Rmu_sc0000493.1_g000037 Rmu_sc0000493.1_g000038 Rmu_sc0001670.1_g000042 Rmu_sc0001670.1_g000043 Rmu_sc0001670.1_g000044 Rmu_sc0002983.1_g000067 Rmu_sc0002983.1_g000074 Rmu_sc0003366.1_g000019 Rmu_sc0003494.1_g000002 Rmu_sc0003712.1_g000014 Rmu_sc0005703.1_g000003 Rmu_sc0005703.1_g000004 Rmu_sc0006429.1_g000014 Rmu_sc0006429.1_g000018 Rmu_sc0006429.1_g000019 Rmu_sc0008701.1_g000007 Rmu_sc0009050.1_g000002 Rmu_sc0009050.1_g000008 Rmu_sc0014587.1_g000001 Rmu_sc0034954.1_g000001 Rmu_ssc0000371.1_g000008 Rmu_ssc0000371.1_g000009 Rmu_ssc0000371.1_g000012 Rmu_ssc0000371.1_g000025 Rmu_ssc0000371.1_g000028
rosa_roxburghii Rroxscaffold_1G00021390 Rroxscaffold_1G00021450 Rroxscaffold_1G00021460 Rroxscaffold_1G00021470 Rroxscaffold_1G00021480 Rroxscaffold_1G00021500 Rroxscaffold_2G00136030 Rroxscaffold_2G00136040 Rroxscaffold_2G00136070 Rroxscaffold_3G00227100 Rroxscaffold_4G00296830 Rroxscaffold_4G00296860 Rroxscaffold_4G00296870 Rroxscaffold_7G00168050 Rroxscaffold_7G00168060 Rroxscaffold_7G00168080 Rroxscaffold_7G00168090
rosa_rugosa Rorug01G0272400 Rorug01G0272500 Rorug01G0272600 Rorug01G0272700 Rorug02G0146800.1 Rorug03G0204200 Rorug03G0204300 Rorug05G0319800 Rorug05G0319900 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug06G0293800 Rorug07G0281800
rosa_samantha Rh5DG412300 Rh5DG412400 Rh6CG421000 Rh7DG427300
rosa_wichuraiana Rw1G025420 Rw1G025430 Rw1G025440 Rw2G015520 Rw5G036240 Rw5G036250 Rw5G036260 Rw5G036270 Rw5G036950 Rw6G035500 Rw6G035510 Rw6G035520 Rw7G036140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1404
AccB1I GGYRCC 1 cut(s) 408
AccB7I CCANNNNNTGG 2 cut(s) 155, 457
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 3 cut(s) 500, 1131, 1396
AccII CGCG 2 cut(s) 1249, 1281
AciI CCGC 6 cut(s) 188, 230, 331, 809, 971, 1162
AclWI GGATC 4 cut(s) 26, 1277, 1295, 1593
AcoI YGGCCR 3 cut(s) 146, 157, 1601
AcsI RAATTY 1 cut(s) 1373
AcuI CTGAAG 1 cut(s) 1073
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 4 cut(s) 224, 365, 850, 1191
AfiI CCNNNNNNNGG 6 cut(s) 133, 155, 457, 484, 819, 1057
AflII CTTAAG 1 cut(s) 172
AflIII ACRYGT 1 cut(s) 1247
AgsI TTSAA 5 cut(s) 933, 1269, 1373, 1472, 1594
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 3 cut(s) 411, 966, 1408
AluBI AGCT 9 cut(s) 247, 263, 320, 341, 672, 707, 959, 1273, 1552
AluI AGCT 9 cut(s) 247, 263, 320, 341, 672, 707, 959, 1273, 1552
Alw21I GWGCWC 2 cut(s) 258, 265
Alw26I GTCTC 1 cut(s) 547
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 4 cut(s) 26, 1277, 1295, 1593
AoxI GGCC 7 cut(s) 146, 157, 179, 774, 810, 1360, 1601
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 3 cut(s) 433, 1175, 1459
ApoI RAATTY 1 cut(s) 1373
AseI ATTAAT 1 cut(s) 1010
Asp700I GAANNNNTTC 2 cut(s) 672, 1326
AspLEI GCGC 3 cut(s) 411, 649, 1281
AspS9I GGNCC 6 cut(s) 122, 179, 227, 398, 811, 1360
AsuC2I CCSGG 2 cut(s) 350, 479
AsuHPI GGTGA 3 cut(s) 295, 502, 1520
AsuII TTCGAA 1 cut(s) 676
AvaII GGWCC 3 cut(s) 122, 227, 398
BaeGI GKGCMC 1 cut(s) 258
BalI TGGCCA 3 cut(s) 148, 159, 1603
BanI GGYRCC 1 cut(s) 408
BanII GRGCYC 2 cut(s) 265, 1021
BbsI GAAGAC 4 cut(s) 791, 1215, 1244, 1541
Bbv12I GWGCWC 2 cut(s) 258, 265
BbvI GCAGC 3 cut(s) 420, 1162, 1446
BccI CCATC 4 cut(s) 50, 604, 797, 1492
BceAI ACGGC 2 cut(s) 660, 804
BciT130I CCWGG 3 cut(s) 413, 968, 1410
BcnI CCSGG 2 cut(s) 350, 479
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 4 cut(s) 203, 824, 980, 1127
BfmI CTRYAG 1 cut(s) 858
BfoI RGCGCY 1 cut(s) 412
BfrI CTTAAG 1 cut(s) 172
BisI GCNGC 5 cut(s) 434, 810, 1163, 1176, 1460
BlsI GCNGC 5 cut(s) 435, 811, 1164, 1177, 1461
BmcAI AGTACT 1 cut(s) 850
Bme1390I CCNGG 5 cut(s) 350, 413, 479, 968, 1410
Bme18I GGWCC 3 cut(s) 122, 227, 398
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 6 cut(s) 122, 179, 227, 398, 811, 1360
BmiI GGNNCC 3 cut(s) 124, 410, 1361
BmrFI CCNGG 5 cut(s) 350, 413, 479, 968, 1410
BmsI GCATC 2 cut(s) 718, 876
BpiI GAAGAC 4 cut(s) 791, 1215, 1244, 1541
BpmI CTGGAG 2 cut(s) 1259, 1568
Bpu14I TTCGAA 1 cut(s) 676
BpuEI CTTGAG 2 cut(s) 452, 1088
BpuMI CCSGG 2 cut(s) 350, 479
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 1 cut(s) 642
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 6 cut(s) 133, 155, 457, 484, 819, 1057
Bse1I ACTGG 3 cut(s) 410, 1452, 1496
BseBI CCWGG 3 cut(s) 413, 968, 1410
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 3 cut(s) 289, 438, 615
BseLI CCNNNNNNNGG 6 cut(s) 133, 155, 457, 484, 819, 1057
BseMII CTCAG 2 cut(s) 738, 1200
BseNI ACTGG 3 cut(s) 410, 1452, 1496
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 3 cut(s) 420, 1162, 1446
BsgI GTGCAG 2 cut(s) 419, 1161
Bsh1236I CGCG 2 cut(s) 1249, 1281
BshFI GGCC 7 cut(s) 148, 159, 181, 776, 812, 1362, 1603
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 258, 265
BsiSI CCGG 2 cut(s) 350, 478
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 6 cut(s) 133, 155, 457, 484, 819, 1057
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 1 cut(s) 1000
BsnI GGCC 7 cut(s) 148, 159, 181, 776, 812, 1362, 1603
Bsp119I TTCGAA 1 cut(s) 676
Bsp1286I GDGCHC 3 cut(s) 258, 265, 1021
Bsp143I GATC 4 cut(s) 31, 1282, 1300, 1585
BspACI CCGC 6 cut(s) 188, 230, 331, 809, 971, 1162
BspANI GGCC 7 cut(s) 148, 159, 181, 776, 812, 1362, 1603
BspCNI CTCAG 2 cut(s) 737, 1199
BspFNI CGCG 2 cut(s) 1249, 1281
BspLI GGNNCC 3 cut(s) 124, 410, 1361
BspPI GGATC 4 cut(s) 26, 1277, 1295, 1593
BspQI GCTCTTC 3 cut(s) 239, 822, 858
BspT104I TTCGAA 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 408
BspTI CTTAAG 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 3 cut(s) 410, 1452, 1496
BssECI CCNNGG 1 cut(s) 642
BssMI GATC 4 cut(s) 31, 1282, 1300, 1585
BssNI GRCGYC 1 cut(s) 409
Bst2UI CCWGG 3 cut(s) 413, 968, 1410
Bst4CI ACNGT 1 cut(s) 895
Bst6I CTCTTC 6 cut(s) 76, 239, 563, 822, 858, 912
BstACI GRCGYC 1 cut(s) 409
BstAFI CTTAAG 1 cut(s) 172
BstBI TTCGAA 1 cut(s) 676
BstC8I GCNNGC 1 cut(s) 1247
BstDEI CTNAG 4 cut(s) 46, 724, 743, 1186
BstDSI CCRYGG 1 cut(s) 642
BstENI CCTNNNNNAGG 1 cut(s) 1055
BstF5I GGATG 3 cut(s) 289, 438, 615
BstFNI CGCG 2 cut(s) 1249, 1281
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 3 cut(s) 411, 649, 1281
BstKTI GATC 4 cut(s) 34, 1285, 1303, 1588
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 4 cut(s) 31, 1282, 1300, 1585
BstMWI GCNNNNNNNGC 7 cut(s) 187, 212, 253, 415, 644, 829, 1212
BstNI CCWGG 3 cut(s) 413, 968, 1410
BstNSI RCATGY 1 cut(s) 966
BstSCI CCNGG 5 cut(s) 348, 411, 477, 966, 1408
BstSFI CTRYAG 1 cut(s) 858
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 2 cut(s) 1249, 1281
BstV1I GCAGC 3 cut(s) 420, 1162, 1446
BstV2I GAAGAC 4 cut(s) 791, 1215, 1244, 1541
BstXI CCANNNNNNTGG 2 cut(s) 1293, 1546
BsuRI GGCC 7 cut(s) 148, 159, 181, 776, 812, 1362, 1603
BtgI CCRYGG 1 cut(s) 642
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 3 cut(s) 289, 438, 615
BtsIMutI CAGTG 2 cut(s) 1008, 1445
Cac8I GCNNGC 1 cut(s) 1247
CfoI GCGC 3 cut(s) 411, 649, 1281
Cfr13I GGNCC 6 cut(s) 122, 179, 227, 398, 811, 1360
CpoI CGGWCCG 1 cut(s) 227
CseI GACGC 1 cut(s) 1238
Csp6I GTAC 4 cut(s) 223, 364, 849, 1190
CspI CGGWCCG 1 cut(s) 227
CviQI GTAC 4 cut(s) 223, 364, 849, 1190
DdeI CTNAG 4 cut(s) 46, 724, 743, 1186
DinI GGCGCC 1 cut(s) 410
DpnI GATC 4 cut(s) 33, 1284, 1302, 1587
DpnII GATC 4 cut(s) 31, 1282, 1300, 1585
DraI TTTAAA 1 cut(s) 594
DrdI GACNNNNNNGTC 1 cut(s) 1404
DseDI GACNNNNNNGTC 1 cut(s) 1404
EaeI YGGCCR 3 cut(s) 146, 157, 1601
Eam1104I CTCTTC 6 cut(s) 76, 239, 563, 822, 858, 912
EarI CTCTTC 6 cut(s) 76, 239, 563, 822, 858, 912
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 2 cut(s) 265, 1021
Eco32I GATATC 1 cut(s) 84
Eco47I GGWCC 3 cut(s) 122, 227, 398
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 263
EcoNI CCTNNNNNAGG 1 cut(s) 1055
EcoO109I RGGNCCY 2 cut(s) 122, 179
EcoRII CCWGG 3 cut(s) 411, 966, 1408
EcoRV GATATC 1 cut(s) 84
EcoT38I GRGCYC 2 cut(s) 265, 1021
EgeI GGCGCC 1 cut(s) 410
EheI GGCGCC 1 cut(s) 410
FalI AAGNNNNNCTT 2 cut(s) 1142, 1174
FaqI GGGAC 1 cut(s) 1000
FblI GTMKAC 3 cut(s) 500, 1131, 1396
Fnu4HI GCNGC 5 cut(s) 434, 810, 1163, 1176, 1460
FokI GGATG 3 cut(s) 296, 425, 622
FriOI GRGCYC 2 cut(s) 265, 1021
Fsp4HI GCNGC 5 cut(s) 434, 810, 1163, 1176, 1460
FspBI CTAG 4 cut(s) 203, 824, 980, 1127
GlaI GCGC 3 cut(s) 410, 648, 1280
GluI GCNGC 5 cut(s) 434, 810, 1163, 1176, 1460
GsuI CTGGAG 2 cut(s) 1259, 1568
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 7 cut(s) 148, 159, 181, 776, 812, 1362, 1603
HapII CCGG 2 cut(s) 350, 478
HgaI GACGC 1 cut(s) 1238
HhaI GCGC 3 cut(s) 411, 649, 1281
Hin1I GRCGYC 1 cut(s) 409
Hin6I GCGC 3 cut(s) 409, 647, 1279
HinP1I GCGC 3 cut(s) 409, 647, 1279
HincII GTYRAC 2 cut(s) 328, 1501
HindII GTYRAC 2 cut(s) 328, 1501
HindIII AAGCTT 4 cut(s) 245, 318, 339, 670
HinfI GANTC 6 cut(s) 1109, 1123, 1199, 1413, 1480, 1580
HpaII CCGG 2 cut(s) 350, 478
HphI GGTGA 3 cut(s) 295, 502, 1520
Hpy166II GTNNAC 8 cut(s) 256, 328, 501, 562, 579, 1132, 1397, 1501
Hpy188I TCNGA 4 cut(s) 727, 1114, 1489, 1585
Hpy188III TCNNGA 4 cut(s) 607, 1051, 1518, 1547
Hpy8I GTNNAC 8 cut(s) 256, 328, 501, 562, 579, 1132, 1397, 1501
HpyAV CCTTC 5 cut(s) 173, 487, 649, 766, 1051
HpyCH4III ACNGT 1 cut(s) 895
HpyCH4IV ACGT 2 cut(s) 474, 1431
HpyCH4V TGCA 9 cut(s) 97, 143, 256, 436, 731, 1178, 1215, 1445, 1462
HpyF10VI GCNNNNNNNGC 7 cut(s) 187, 212, 253, 415, 644, 829, 1212
HpyF3I CTNAG 4 cut(s) 46, 724, 743, 1186
HpySE526I ACGT 2 cut(s) 474, 1431
Hsp92I GRCGYC 1 cut(s) 409
HspAI GCGC 3 cut(s) 409, 647, 1279
KasI GGCGCC 1 cut(s) 408
Kzo9I GATC 4 cut(s) 31, 1282, 1300, 1585
LguI GCTCTTC 3 cut(s) 239, 822, 858
LmnI GCTCC 3 cut(s) 526, 1278, 1549
Lsp1109I GCAGC 3 cut(s) 420, 1162, 1446
LweI GCATC 2 cut(s) 718, 876
MaeI CTAG 4 cut(s) 203, 824, 980, 1127
MaeII ACGT 2 cut(s) 474, 1431
MaeIII GTNAC 3 cut(s) 1003, 1223, 1432
MalI GATC 4 cut(s) 33, 1284, 1302, 1587
MbiI CCGCTC 1 cut(s) 232
MboI GATC 4 cut(s) 31, 1282, 1300, 1585
MhlI GDGCHC 3 cut(s) 258, 265, 1021
MlsI TGGCCA 3 cut(s) 148, 159, 1603
MluCI AATT 7 cut(s) 713, 853, 933, 948, 1373, 1473, 1557
MluI ACGCGT 1 cut(s) 1247
MluNI TGGCCA 3 cut(s) 148, 159, 1603
Mly113I GGCGCC 1 cut(s) 409
MlyI GAGTC 1 cut(s) 1474
MmeI TCCRAC 6 cut(s) 59, 438, 471, 598, 732, 1339
Mox20I TGGCCA 3 cut(s) 148, 159, 1603
MroXI GAANNNNTTC 2 cut(s) 672, 1326
MscI TGGCCA 3 cut(s) 148, 159, 1603
MseI TTAA 6 cut(s) 173, 344, 587, 593, 951, 1010
MslI CAYNNNNRTG 3 cut(s) 881, 1344, 1575
Msp20I TGGCCA 3 cut(s) 148, 159, 1603
MspCI CTTAAG 1 cut(s) 172
MspI CCGG 2 cut(s) 350, 478
MspR9I CCNGG 5 cut(s) 350, 413, 479, 968, 1410
MvaI CCWGG 3 cut(s) 413, 968, 1410
MvnI CGCG 2 cut(s) 1249, 1281
MwoI GCNNNNNNNGC 7 cut(s) 187, 212, 253, 415, 644, 829, 1212
NarI GGCGCC 1 cut(s) 409
NciI CCSGG 2 cut(s) 350, 479
NdeII GATC 4 cut(s) 31, 1282, 1300, 1585
NlaIV GGNNCC 3 cut(s) 124, 410, 1361
NmuCI GTSAC 1 cut(s) 1003
NspI RCATGY 1 cut(s) 966
NspV TTCGAA 1 cut(s) 676
PciSI GCTCTTC 3 cut(s) 239, 822, 858
PcsI WCGNNNNNNNCGW 1 cut(s) 1428
PdmI GAANNNNTTC 2 cut(s) 672, 1326
PfeI GAWTC 5 cut(s) 1109, 1123, 1199, 1413, 1580
PflMI CCANNNNNTGG 2 cut(s) 155, 457
PfoI TCCNGGA 2 cut(s) 477, 1408
PkrI GCNGC 5 cut(s) 435, 811, 1164, 1177, 1461
PleI GAGTC 1 cut(s) 1474
PluTI GGCGCC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 1474
PpuMI RGGWCCY 1 cut(s) 122
PshBI ATTAAT 1 cut(s) 1010
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 3 cut(s) 411, 966, 1408
PspGI CCWGG 3 cut(s) 411, 966, 1408
PspN4I GGNNCC 3 cut(s) 124, 410, 1361
PspPI GGNCC 6 cut(s) 122, 179, 227, 398, 811, 1360
PspPPI RGGWCCY 1 cut(s) 122
RsaI GTAC 4 cut(s) 224, 365, 850, 1191
RsaNI GTAC 4 cut(s) 223, 364, 849, 1190
RseI CAYNNNNRTG 3 cut(s) 881, 1344, 1575
Rsr2I CGGWCCG 1 cut(s) 227
RsrII CGGWCCG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 265
SapI GCTCTTC 3 cut(s) 239, 822, 858
SaqAI TTAA 6 cut(s) 173, 344, 587, 593, 951, 1010
SatI GCNGC 5 cut(s) 434, 810, 1163, 1176, 1460
Sau3AI GATC 4 cut(s) 31, 1282, 1300, 1585
Sau96I GGNCC 6 cut(s) 122, 179, 227, 398, 811, 1360
ScaI AGTACT 1 cut(s) 850
SchI GAGTC 1 cut(s) 1474
ScrFI CCNGG 5 cut(s) 350, 413, 479, 968, 1410
SduI GDGCHC 3 cut(s) 258, 265, 1021
SfaNI GCATC 2 cut(s) 718, 876
SfcI CTRYAG 1 cut(s) 858
SfoI GGCGCC 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 676
SinI GGWCC 3 cut(s) 122, 227, 398
SmiMI CAYNNNNRTG 3 cut(s) 881, 1344, 1575
SmlI CTYRAG 3 cut(s) 172, 467, 1067
SmoI CTYRAG 3 cut(s) 172, 467, 1067
Sse9I AATT 7 cut(s) 713, 853, 933, 948, 1373, 1473, 1557
SsiI CCGC 6 cut(s) 188, 230, 331, 809, 971, 1162
SspDI GGCGCC 1 cut(s) 408
SspMI CTAG 4 cut(s) 203, 824, 980, 1127
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 5 cut(s) 348, 411, 477, 966, 1408
TaaI ACNGT 1 cut(s) 895
TaiI ACGT 2 cut(s) 477, 1434
TaqI TCGA 3 cut(s) 676, 801, 1299
TasI AATT 7 cut(s) 713, 853, 933, 948, 1373, 1473, 1557
TatI WGTACW 1 cut(s) 848
TauI GCSGC 2 cut(s) 812, 1165
TfiI GAWTC 5 cut(s) 1109, 1123, 1199, 1413, 1580
Tru1I TTAA 6 cut(s) 173, 344, 587, 593, 951, 1010
Tru9I TTAA 6 cut(s) 173, 344, 587, 593, 951, 1010
TscAI CASTG 2 cut(s) 1008, 1452
TseFI GTSAC 1 cut(s) 1003
TseI GCWGC 3 cut(s) 433, 1175, 1459
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 8 cut(s) 581, 794, 865, 1122, 1212, 1506, 1547, 1593
TspGWI ACGGA 4 cut(s) 64, 240, 411, 500
TspRI CASTG 2 cut(s) 1008, 1452
Van91I CCANNNNNTGG 2 cut(s) 155, 457
Vha464I CTTAAG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 3 cut(s) 122, 227, 398
VspI ATTAAT 1 cut(s) 1010
XagI CCTNNNNNAGG 1 cut(s) 1055
XapI RAATTY 1 cut(s) 1373
XceI RCATGY 1 cut(s) 966
XcmI CCANNNNNNNNNTGG 2 cut(s) 454, 744
XmiI GTMKAC 3 cut(s) 500, 1131, 1396
XmnI GAANNNNTTC 2 cut(s) 672, 1326
XspI CTAG 4 cut(s) 203, 824, 980, 1127
ZrmI AGTACT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.